CN108077263B - A kind of cotton low temperature regulator and preparation method thereof and application method - Google Patents

A kind of cotton low temperature regulator and preparation method thereof and application method Download PDF

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Publication number
CN108077263B
CN108077263B CN201711385588.7A CN201711385588A CN108077263B CN 108077263 B CN108077263 B CN 108077263B CN 201711385588 A CN201711385588 A CN 201711385588A CN 108077263 B CN108077263 B CN 108077263B
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low temperature
cotton
temperature regulator
seedling
tween
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CN108077263A (en
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李淑叶
赵新华
庞朝友
张思平
刘绍东
沈倩
葛常伟
陈静
马慧娟
张小萌
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Institute of Cotton Research of Chinese Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/22Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • A01G2013/004Liquid mulch

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
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  • Biodiversity & Conservation Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
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  • Toxicology (AREA)
  • Agronomy & Crop Science (AREA)
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Abstract

The present invention provides a kind of cotton low temperature regulator and preparation method thereof and application method, 24- epi-brassinolide helps to improve cotton seedling low temperature tolerance ability in cotton low temperature regulator provided by the invention;Under the dissolution of ethyl alcohol, it is ensured that 24- epi-brassinolide and azone are completely dissolved in water, and then are facilitated each component and absorbed as a solution by cotton seedling.Azone plays the role of bleeding agent, it can promote absorption of the cotton leaf to the low temperature regulator, so that the other components such as 24- epi-brassinolide are penetrated into cotton leaf, the drug effect of the low temperature regulator is improved jointly with tween and organosilicon, and then improve the low temperature resistant power of cotton seedling.Embodiment the result shows that, the low temperature tolerance ability of cotton in seedling stage can be improved in cotton low temperature regulator provided by the invention, slow down or avoid injury of the low temperature to cotton seedling cell membrane, alleviate destruction of the low temperature to chlorophyll, and then cotton seedling is made to keep relatively high photosynthetic capacity under low temperature stress, to promote plant growth.

Description

A kind of cotton low temperature regulator and preparation method thereof and application method
Technical field
The invention belongs to cotton Chilling stress control technique field more particularly to a kind of cotton low temperature regulator and its preparations Method and application method.
Background technique
Since mid-term the 1990s, Northwest inland cotton region becomes national cotton advantage producing region.In recent years 60% Output of cotton is come to Northwest inland cotton region, but Northwest inland frost-free period is short, is subject to early period to damage to plants caused by sudden drop in temperature and frost damage.Cotton in seedling stage Meeting low temperature will cause weak cotton seedling, growth retardation, wilting or yellow, easily formation " grown-ups and children's seedling ";And low temperature can make cotton plant susceptible Dye fungi and induce various diseases, cause crop field be short of seedling disconnected ridge even replay, cause serious economic loss.
Covering with ground sheeting plant cotton technology or raising seedling of cotton transplantation technique is mostly used to realize the regulation of cotton Chilling stress now.
It is a kind of by increasing ground temperature realization cotton planting early enough, the technology that early emergence, seedling stage heat that cotton is planted in covering with ground sheeting.Often Covering with ground sheeting plant cotton technology avoids cotton seedling by injury from low temperature, puts in cotton to increase cotton in seedling stage temperature as far as possible Still overlay film to cotton late growth stage just takes off film after seedling, causes to take off the increase of film difficulty, broken residual mulch is trapped in field.Covering with ground sheeting Inevitably will cause serious " white pollution ", contaminated soil environment causes farmland quality to decline, influence crop yield and Quality finally will lead to the unsustainable property of agricultural production.
Raising seedling of cotton transplantation technique is one kind in control environment nursery, is transplanted to big Tanaka's again after cotton seedling physically well develops Mode can effectively avoid cotton in seedling stage from meeting with injury from low temperature while extending cotton development stage.Traditional seedling transplanting technique Initial stage nursery is carried out using nutritive cube, but that there is recruitments is more, manages cumbersome, labor intensity for traditional nutrition bowl seedling transplanting Greatly, be not suitable for the problem of Rural Areas at Present labor shortage.Novel seedling raising transplantation technique is to integrate nursery base substitution conventional nutraceutical Alms bowl, although solving the problems, such as that feeding block seedlings raising exists to a certain extent, Novel seedling raising transplants the with high content of technology of requirement, And comprehensive nursery base is needed, cotton planting cost can be increased by a relatively large margin, the receipts of cotton grower will necessarily be reduced relative to live streaming Enter.
Summary of the invention
In view of this, the purpose of the present invention is to provide cotton low temperature regulator and preparation method thereof and application method, this Cotton in seedling stage can be effectively alleviated or avoided by injury from low temperature in the regulator that invention provides, and operates letter in use process It is single, it not will cause the pollution to environment, the increase of production cost will not be caused.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of cotton low temperature regulators, comprising: 24- epi-brassinolide, tween, azone, has ethyl alcohol Machine silicon and water;The quality of the 24- epi-brassinolide, the volume of ethyl alcohol, the volume of tween, the volume of azone, organosilicon Quality and the volume ratio of water are (0.05~0.5) mg:(0.5~2) mL:(0.8~1.5) mL:(0.5~2.5) mL:(0.28~ 0.32) g:1000mL.
Preferably, the quality, the volume of ethyl alcohol of the 24- epi-brassinolide, the volume of tween, the volume of azone, have The quality of machine silicon and the volume ratio of water are (0.1~0.45) mg:(1.0~1.5) mL:(1.0~1.2) mL:(1.0~2.0) mL: 0.30g:1000mL.
Preferably, the organosilicon is poly- trisiloxanes;
The tween is one of polysorbas20, polysorbate40 and Tween 80 or a variety of.
The present invention provides the preparation methods of cotton low temperature regulator described in above-mentioned technical proposal, comprising the following steps:
(1) azone is added after the 24- epi-brassinolide being dissolved in ethyl alcohol, obtains mixed liquor;
(2) mixed liquor and the water mixing obtained the step (1), obtains dilution;
(3) dilution that the step (2) obtains is mixed with tween and organosilicon, obtains cotton low temperature regulator.
The present invention also provides preparation sides described in cotton low temperature regulator described in above-mentioned technical proposal or above-mentioned technical proposal The application method for the cotton low temperature regulator that method is prepared includes: in the pre- preceding 12~36h of cooling, by the cotton low temperature tune Section agent is applied on cotton seedling leaf.
Preferably, the amount of application of the cotton low temperature regulator is with soil where the cotton seedling of cotton low temperature regulator to be administered Area on the basis of count, the amount of application of the cotton low temperature regulator is 450~600kg/hm2
Preferably, when the pre- cooling extent is at 2 DEG C or less, the amount of application of the cotton low temperature regulator is [450kg/hm2,475kg/hm2];
When the pre- cooling extent is (2 DEG C, 5 DEG C] when, the usage amount of the cotton low temperature regulator is (475kg/hm2, 520kg/hm2];
When the pre- cooling extent is (5 DEG C, 10 DEG C), the usage amount of the cotton low temperature regulator is (520kg/ hm2,550kg/hm2];
When the pre- cooling extent is 10 DEG C or more, the usage amount of the cotton low temperature regulator is (550kg/hm2, 600kg/hm2]。
Preferably, the time of the application is 8 a.m. before or after at 5 points in afternoon.
Preferably, the method for application is flat spray.
The present invention provides a kind of cotton low temperature regulators, comprising: 24- epi-brassinolide, tween, azone, has ethyl alcohol Machine silicon and water;The quality of the 24- epi-brassinolide, the volume of ethyl alcohol, the volume of tween, the volume of azone, organosilicon Quality and the volume ratio of water are (0.05~0.5) mg:(0.5~2) mL:(0.8~1.5) mL:(0.5~2.5) mL:(0.28~ 0.32) g:1000mL.In the present invention, 24- epi-brassinolide helps to improve cotton seedling low temperature tolerance ability;In the dissolution of ethyl alcohol Under effect, it is ensured that 24- epi-brassinolide and azone are completely dissolved in water, and then facilitate each component as a solution by Cotton seedling absorbs.Also, azone plays the role of bleeding agent, can promote absorption of the cotton leaf to the low temperature regulator, so that 24- The other components such as epi-brassinolide are penetrated into cotton leaf, improve the medicine of the low temperature regulator jointly with organosilicon and tween Effect, and then improve the low temperature resistant power of cotton seedling.Embodiment the result shows that, cotton low temperature regulator provided by the invention can mention The low temperature tolerance ability of high cotton in seedling stage slows down or avoids injury of the low temperature to cotton seedling cell membrane, alleviates low temperature and breaks to chlorophyll It is bad, and then cotton seedling is made to keep relatively high photosynthetic capacity under low temperature stress, to promote plant growth.
Detailed description of the invention
Fig. 1 is that experiment station, the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute base is per day from April 5th, 2017 to April 15 Temperature variation curve.
Specific embodiment
The present invention provides a kind of cotton low temperature regulators, comprising: 24- epi-brassinolide, tween, azone, has ethyl alcohol Machine silicon and water;The quality of the 24- epi-brassinolide, the volume of ethyl alcohol, the volume of tween, the volume of azone, organosilicon Quality and the volume ratio of water are (0.05~0.5) mg:(0.5~2) mL:(0.8~1.5) mL:(0.5~2.5) mL:(0.28~ 0.32) g:1000mL.
Cotton low temperature regulator provided by the invention includes 24- epi-brassinolide.In the present invention, the 24- table oil Rape lactone helps to improve cotton seedling low temperature tolerance ability.The present invention is to the specific source of the 24- epi-brassinolide without spy It is different to require, using commercial goods well-known to those skilled in the art.In terms of the volume of water, contain in every 1000mL water The quality of 24- epi-brassinolide be 0.05~0.5mg, further preferably 0.1~0.45mg, more preferably 0.2~ 0.3mg。
Cotton low temperature regulator provided by the invention includes ethyl alcohol;In the present invention, the ethyl alcohol is preferably dehydrated alcohol Or mass concentration is 98% ethyl alcohol, most preferably dehydrated alcohol.In the present invention, the ethyl alcohol is played to 24- table rape element The dissolution of lactone and azone convenient for improving the dissolubility in cotton low temperature regulator, and then ensures that 24- epi-brassinolide is made It gives full play to, improves the low temperature tolerance ability of cotton in seedling stage, can slow down or avoid low temperature injury caused by cotton seedling.The present invention There is no particular/special requirement to the source of the ethyl alcohol, using commercial goods well-known to those skilled in the art.With the body of water Product is counted, and the volume containing ethyl alcohol is 0.5~2mL, further preferably 1.0~1.5mL, more preferably 0.2 in every 1000mL water ~0.3mL.
Cotton low temperature regulator provided by the invention includes organosilicon;In the present invention, the organosilicon is preferably poly- three Siloxanes.The present invention does not have particular/special requirement to the source of the organosilicon, using commercially available quotient well-known to those skilled in the art Product.In the present invention, the organosilicon plays the role of spreader-sticker, improves the cotton low temperature regulator in cotton leaf Developing power helps the cotton low temperature regulator and is fully absorbed by cotton leaf.In terms of the volume of water, contain in every 1000mL water The quality for having organosilicon is 0.28~0.32g, further preferably 0.29~0.30g.
Cotton low temperature regulator provided by the invention includes tween;In the present invention, the tween is preferably polysorbas20, spits Warm one of 40 and Tween 80 or a variety of.The present invention does not have particular/special requirement to the specific source of the tween, using this field Commercial goods known to technical staff.In the present invention, the tween is as emulsifier, so that other components can be It is even to be scattered in solution.In terms of the volume of water, the volume containing tween is 0.8~1.5mL in every 1000mL water, further preferably For 1.0~1.2mL, more preferably 1.05~1.15mL.
Cotton low temperature regulator provided by the invention includes azone.In the present invention, the azone plays the work of bleeding agent With, can promote absorption of the cotton leaf to the low temperature regulator so that the other components such as 24- epi-brassinolide penetrate into cotton leaf In piece, the drug effect of the low temperature regulator is improved jointly with tween and organosilicon, and then improve the low temperature resistant power of cotton seedling.The present invention There is no particular/special requirement to the specific source of the azone, using commercial goods well-known to those skilled in the art.With water Stereometer, the volume containing azone is 0.5~2.5mL, further preferably 1.0~2.0mL in every 1000mL water, more preferably For 1.5~1.8mL.
The present invention also provides the preparation methods of cotton low temperature regulator described in above-mentioned technical proposal, comprising the following steps:
(1) azone is added after the 24- epi-brassinolide being dissolved in ethyl alcohol, obtains mixed liquor;
(2) mixed liquor and the water mixing obtained the step (1), obtains dilution;
(3) dilution that the step (2) obtains is mixed with tween and organosilicon, obtains cotton low temperature regulator.
Azone is added after the 24- epi-brassinolide is dissolved in ethyl alcohol in the present invention, obtains mixed liquor.The present invention is by institute It states 24- epi-brassinolide and azone is first dissolved in ethyl alcohol, it is ensured that it is subsequent to be completely dissolved in water, as a solution It is absorbed by cotton seedling.The 24- epi-brassinolide is preferably dissolved in ethyl alcohol by the present invention under agitation;The present invention is to institute Stating stirring condition does not have particular/special requirement, and the dissolution of the 24- epi-brassinolide in ethanol can be promoted by being subject to.In the present invention In, the azone is preferably added under agitation, and the present invention does not have particular/special requirement to the stirring condition, described in it can promote Subject to the dissolution of azone in ethanol.
After obtaining mixed liquor, the present invention mixes the mixed liquor and water, obtains dilution.In the present invention, described mixed The mixing for closing liquid and water preferably carries out under agitation;The time of the stirring is preferably 30s~60s.In the present invention, institute Water is preferably added in mixed liquor by the mixing for stating mixed liquor and water;The present invention is using first by 24- epi-brassinolide and nitrogen Ketone is dissolved in ethyl alcohol, then the mode mixed with water obtains dilution, helps to be dissolved in the 24- epi-brassinolide in ethyl alcohol It is completely dissolved in water with azone, solubility is low in water for solution 24- epi-brassinolide and azone, and caused low temperature is adjusted Component is difficult to the problem of being absorbed by cotton seedling in agent.
After obtaining dilution, the present invention mixes the dilution with tween and organosilicon, obtains cotton low temperature regulator. In the present invention, the mixing of the dilution, tween and organosilicon preferably first mixes the tween and dilution, then by institute Organosilicon is stated to be added in the dilution dissolved with tween.In the present invention, the mixing of the tween and dilution is preferably being stirred It is carried out under the conditions of mixing;The time of the stirring is preferably 30s~60s.The present invention realizes tween by controlling the stirring condition Dissolution in dilution, and can be avoided and generate a large amount of foams.In the present invention, the addition of the organosilicon is preferably being stirred It is carried out under the conditions of mixing, the present invention does not have particular/special requirement to the stirring condition, can promote the dissolution of organosilicon.
In the cotton low temperature regulator preparation process, the dosage of each component of the present invention is can obtain aforementioned techniques Constituent mass described in scheme is than subject to desired cotton low temperature regulator.
The present invention also provides preparation sides described in cotton low temperature regulator described in above-mentioned technical proposal or above-mentioned technical proposal The application method for the cotton low temperature regulator that method is prepared includes: in the pre- preceding 12~36h of cooling, by the cotton low temperature tune Section agent is applied on cotton seedling leaf.
The cotton low temperature regulator is applied on cotton seedling leaf by the present invention in the pre- preceding 12~36h of cooling;Preferably drop 15 before temperature~for 24 hours, further preferably 18~21h.In the present invention, the time of the application be preferably before 8 a.m. or After at 5 points in afternoon, further preferably 5 points~8 points of 5 points~8 points of the morning or afternoon.The present invention selects suitable administration time, It can prevent from causing reagent to evaporate when sunlight intensity is big, reduce drug effect.In the present invention, the method for application is preferably flat Spray, further preferably in the flat spray of the tow sides of cotton seedling leaf.The present invention is not special to the specific embodiment of the application It is required that the method for application using liquid preparation well-known to those skilled in the art on cotton leaf;Such as aircraft spray Medicine or mechanical spray.The present invention does not have particular/special requirement to the seedling stage of cotton seedling to be administered, improves low temperature with any required before cooling down in advance The cotton Miao Jun of resistance can.
In the present invention, the amount of application of the cotton low temperature regulator is where the cotton seedling of cotton low temperature regulator to be administered It is counted on the basis of the area of soil, the amount of application of the cotton low temperature regulator is preferably 450~600kg/hm2
The present invention further preferably determines the amount of application of the cotton low temperature regulator according to cooling extent, both can guarantee that cotton seedling was anti- The raising of low temperature capability, and can ensure that the efficient utilization of the cotton low temperature regulator.In the present invention, when the pre- cooling width For degree at 2 DEG C or less, the amount of application of the cotton low temperature regulator is [450kg/hm2,475kg/hm2];When the pre- cooling width Degree for (2 DEG C, 5 DEG C] when, the usage amount of the cotton low temperature regulator is (475kg/hm2,520kg/hm2];When the pre- cooling When amplitude is (5 DEG C, 10 DEG C), the usage amount of the cotton low temperature regulator is (520kg/hm2,550kg/hm2];When described pre- When cooling extent is 10 DEG C or more, the usage amount of the cotton low temperature regulator is (550kg/hm2,600kg/hm2]。
24- epi-brassinolide helps to improve cotton seedling low temperature tolerance ability in cotton low temperature regulator provided by the invention; Under the dissolution of ethyl alcohol, it is ensured that 24- epi-brassinolide and azone are completely dissolved in water, and then facilitate each component with The form of solution is absorbed by cotton seedling.Also, azone plays the role of bleeding agent, can promote cotton leaf to the low temperature regulator It absorbs, so that the other components such as 24- epi-brassinolide are penetrated into cotton leaf, improves the low temperature jointly with organosilicon and tween The drug effect of regulator, and then improve the low temperature resistant power of cotton seedling.
The low temperature resistant ability of cotton seedling can be effectively improved by applying cotton low temperature regulator provided by the invention, be put in cotton Mulch can be taken off in time after seedling, directly cotton low temperature regulator of the present invention is applied according to preceding solution, without passing through ground The damage for being protected from low temperature of film not only improves the rate of recovery of mulch, moreover it is possible to reduce the residual amount of mulch in arable land;It can also Culturing and transplanting seedlings recruitment caused by avoiding by the way of culturing and transplanting seedlings is more, manages cumbersome, large labor intensity and input cost It improves.
Below with reference to embodiment to provided by the present invention for cotton low temperature regulator and preparation method thereof and application method It is described in detail, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
Prepare cotton low temperature regulator:
It weighs 2mL dehydrated alcohol and is put into spare in stirring container, then weigh 0.3mg24- epi-brassinolide, be slowly added to In spare dehydrated alcohol, stirring 30s is made it completely dissolved.
2.5mL azone is weighed, is slowly added in the dehydrated alcohol for having dissolved 24- epi-brassinolide, continues to stir 60s, It makes it completely dissolved, obtains mixed liquor.
It weighs in the mixed liquor that 1000mL water is added, after stirring and evenly mixing, adds 1.5mL polysorbas20, stir 30s, Avoid spend from stirring a large amount of foams of generation more.
It weighs the poly- trisiloxanes of 0.32g to be added in previous solu, obtains cotton low temperature regulator after mixing evenly.
Embodiment 2
Cotton low temperature regulator is prepared in the way of embodiment 1, difference is that the dosage of each raw material component is respectively as follows: The 24- epi-brassinolide of 0.05mg, 0.5mL dehydrated alcohol, 1mL azone, 1L water, 0.8mL polysorbas20, poly- three silicon of 0.28g Oxygen alkane.
Embodiment 3
Cotton low temperature regulator is prepared in the way of embodiment 1, difference is that the dosage of each raw material component is respectively as follows: The 24- epi-brassinolide of 0.1mg, 1mL dehydrated alcohol, 1mL azone, 1L water, 0.8mL polysorbas20, the poly- three silicon oxygen of 0.28g Alkane.
Embodiment 4
Cotton low temperature regulator is prepared in the way of embodiment 1, difference is that the dosage of each raw material component is respectively as follows: The 24- epi-brassinolide of 0.2mg, 1.5mL dehydrated alcohol, 1mL azone, 1L water, 0.8mL polysorbas20, the poly- three silicon oxygen of 0.28g Alkane.
Embodiment 5
Cotton low temperature regulator is prepared in the way of embodiment 1, difference is that the dosage of each raw material component is respectively as follows: The 24- epi-brassinolide of 0.5mg, 2mL dehydrated alcohol, 1mL azone, 1L water, 0.8mL polysorbas20, the poly- three silicon oxygen of 0.28g Alkane.
Embodiment 6
The application effect for the cotton low temperature regulator that embodiment 2~5 is prepared is tested:
It is tested in March, 2017~April in experiment station, the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute base (Anyang in Henan province County, 36 ° of 07 ' N, 114 ° of 22 ' E) it carries out.Wherein, the temperature change in 5~15 April of this area is as shown in Figure 1.
Grind 28 with Shandong cotton as material to be tested, sow within Yu Sanyue 26th and simultaneously covered with double mulch film in advance, after emergence on April 5 and Shi Fangmiao.Using randomized complete-block design, 3 pieces of regions are arranged in every district's groups, are convenient for 3 repetitions.
Cooling the previous day is to be sprayed by machinery April 7, sprays distilled water (CK) and embodiment respectively on cotton miaoye face 2~5 cottons prepared adjust preparation, are uniformly sprayed on cotton leaf, Field information dosage are as follows: 500kg/ using flat spray mode hm2.After 3 days low temperature stress, Yu Siyue 11 days measures the relative conductivity of cotton seedling leaf, chlorophyll content, photosynthetic respectively Performance index, photosynthetic rate and plant weights index, test result are as shown in table 1.
The performance indicator of cotton seedling after the regulator that table 1 applies sprinkling CK respectively and embodiment 2~5 is prepared
As can be seen from the data in table 1, the regulator prepared of the present invention is sprayed before cooling on cotton miaoye face, with application go from The cotton seedling of sub- water is compareed, and the relative conductivity of the cotton seedling leaf of the prepared regulator of the application present invention decreases, table The bright regulator can mitigate injury of the low temperature to cotton seedling leaf cell membrane.The cotton seedling leaf of the prepared regulator of the application present invention Chlorophyll content, photochemical properties index and Net Photosynthetic Rate increase, show that the regulator can mitigate low temperature to cotton Spend photosynthetic influence.The plant weights of the cotton seedling leaf of the prepared regulator of the application present invention increased, and show the tune Section agent can alleviate inhibition of the low temperature to cotton seedling growth and development.As it can be seen that cotton low temperature regulator provided by the invention can be improved The low temperature tolerance ability of cotton in seedling stage slows down or avoids injury of the low temperature to cotton seedling cell membrane, alleviates destruction of the low temperature to chlorophyll, And then cotton seedling is made to keep relatively high photosynthetic capacity under low temperature stress, to promote plant growth.
As seen from the above embodiment, the low temperature resistant energy of cotton in seedling stage can be improved in cotton low temperature regulator provided by the invention Power slows down or avoids injury of the low temperature to cotton seedling cell membrane, alleviates destruction of the low temperature to chlorophyll, and then coerce cotton seedling in low temperature Compel the relatively high photosynthetic capacity of lower holding, to promote cotton growth.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered It is considered as protection scope of the present invention.

Claims (9)

1. a kind of cotton low temperature regulator, comprising: 24- epi-brassinolide, ethyl alcohol, tween, azone, organosilicon and water;It is described Quality, the volume of ethyl alcohol, the volume of tween, the volume of azone, the volume of the quality of organosilicon and water of 24- epi-brassinolide Than for (0.05~0.5) mg:(0.5~2) mL:(0.8~1.5) mL:(0.5~2.5) mL:(0.28~0.32) g:1000mL;
The preparation method of the cotton low temperature regulator, comprising the following steps:
(1) azone is added after the 24- epi-brassinolide being dissolved in ethyl alcohol, obtains mixed liquor;
(2) mixed liquor and the water mixing obtained the step (1), obtains dilution;
(3) dilution that the step (2) obtains is mixed with tween and organosilicon, obtains cotton low temperature regulator;
The dilution first mixes the tween and dilution with tween and being mixed into for organosilicon, then the organosilicon is added Enter into the dilution dissolved with tween.
2. cotton low temperature regulator according to claim 1, which is characterized in that the quality of the 24- epi-brassinolide, The volume of ethyl alcohol, the volume of tween, the volume of azone, the volume ratio of the quality of organosilicon and water are (0.1~0.45) mg:(1.0 ~1.5) mL:(1.0~1.2) mL:(1.0~2.0) mL:0.30g:1000mL.
3. cotton low temperature regulator according to claim 1 or 2, which is characterized in that the organosilicon is poly- trisiloxanes;
The tween is one of polysorbas20, polysorbate40 and Tween 80 or a variety of.
4. the preparation method of any one of claims 1 to 3 cotton low temperature regulator, comprising the following steps:
(1) azone is added after the 24- epi-brassinolide being dissolved in ethyl alcohol, obtains mixed liquor;
(2) mixed liquor and the water mixing obtained the step (1), obtains dilution;
(3) dilution that the step (2) obtains is mixed with tween and organosilicon, obtains cotton low temperature regulator.
5. the cotton that preparation method described in any one of claims 1 to 3 cotton low temperature regulator or claim 4 is prepared The application method of flower low temperature regulator includes: in the pre- preceding 12~36h of cooling, the cotton low temperature regulator being applied to cotton seedling On blade.
6. application method according to claim 5, which is characterized in that the amount of application of the cotton low temperature regulator is wait apply It is counted on the basis of the area of soil where the cotton seedling of cotton low temperature regulator, the amount of application of the cotton low temperature regulator is 450 ~600kg/hm2
7. application method according to claim 6, which is characterized in that described when the pre- cooling extent is at 2 DEG C or less The amount of application of cotton low temperature regulator is [450kg/hm2,475kg/hm2];
When the pre- cooling extent is (2 DEG C, 5 DEG C] when, the usage amount of the cotton low temperature regulator is (475kg/hm2, 520kg/hm2];
When the pre- cooling extent is (5 DEG C, 10 DEG C), the usage amount of the cotton low temperature regulator is (520kg/hm2, 550kg/hm2];
When the pre- cooling extent is 10 DEG C or more, the usage amount of the cotton low temperature regulator is (550kg/hm2,600kg/ hm2]。
8. application method according to claim 5, which is characterized in that the time of the application be 8 a.m. before or under After 5 points of noon.
9. application method according to claim 5, which is characterized in that the mode of the application is flat spray.
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