CN108066805B - Epsilon-polylysine bionic antibacterial film and preparation and application thereof - Google Patents
Epsilon-polylysine bionic antibacterial film and preparation and application thereof Download PDFInfo
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Abstract
The invention relates to an epsilon-polylysine bionic antibacterial film and a preparation method thereof, which are characterized in that a gelatinizing aqueous solution containing epsilon-polylysine, chitosan, hyaluronic acid, gelatin, polyvinyl alcohol and glycerol is prepared, then a cross-linking agent is added to carry out cross-linking reaction in a patterned template to obtain gel, and the epsilon-polylysine bionic antibacterial film is obtained after neutralization, cleaning and drying. The epsilon-polylysine bionic antibacterial membrane has good antibacterial and moisturizing properties, and in addition, the composition of the epsilon-polylysine bionic antibacterial membrane is similar to that of an extracellular matrix and has a patterned surface, so that the epsilon-polylysine bionic antibacterial membrane can promote the adhesion, growth and migration of epithelial cells. Therefore, the epsilon-polylysine bionic antibacterial film can be used as a skin wound dressing, and the wound repair effect is improved.
Description
Technical Field
The invention relates to the field of regenerative medicine, in particular to an epsilon-polylysine bionic antibacterial film and preparation and application thereof.
Background
The skin, which is a tissue of the body surface surrounding the outside of muscles, is the largest organ of the human body, and covers the whole body, protecting various tissues and organs in the body from physical, mechanical, chemical and pathogenic microorganisms. Burn, scald and trauma are common skin injuries, the partial barrier function of the skin is lost due to the injuries, external pathogenic bacteria easily enter the body directly to cause skin infection and even necrosis, and the consequence of large-area skin injury is more serious.
The skin wound dressing is a common treatment means for skin injury, has the characteristics of water absorption, moisture retention and air permeability, can form a temporary barrier at the wound, separates the skin wound from the external environment, and provides a moist and air-permeable environment for skin repair. Chitosan has good biocompatibility and degradability, has certain antibacterial, anti-inflammatory and hemostatic effects, and also has good film forming property, so the chitosan is one of the main raw materials of skin wound dressing, and various wound repair films based on chitosan are already on the market, such as chitosan medical biological wound repair dressing of the Fufu medical group medical supplies Limited company, "Sufu medical chitosan wound repair film" of the Wuhan Dazhenggao biological medicine Limited company, and "chitosan functional dressing" of the Guangdong Taibao medical science and technology division Limited company. However, current chitosan-based wound dressings suffer from two serious drawbacks: 1) chitosan is widely existed in the nature, and flora has tolerance effect on the chitosan, so the bacteriostatic action of the existing dressing is limited; 2) the difference between the composition and the interface morphology of the dressing and the extracellular matrix is large, and the dressing can not effectively guide the adhesion, growth, migration and fusion of skin epithelial cells around the wound surface.
In order to overcome the bottleneck of the prior art, the invention discloses an epsilon-polylysine bionic antibacterial film. Epsilon-polylysine is a lysine homopolymer produced by microorganisms, is a polycation polypeptide which is safe and nontoxic to human bodies, can inhibit gram-negative bacteria, gram-positive bacteria, fungi and the like, even certain viruses, has a wide antibacterial spectrum, and has been widely applied as a food preservative in China, Japan, Korea, United states and other countries. However, although epsilon-polylysine has good bacteriostatic properties, it has not been applied to wound dressings on the surface of the skin. The epsilon-polylysine and the chitosan are compounded for use, the epsilon-polylysine bionic antibacterial film is prepared, and the antibacterial activity of the wound dressing is further improved. In addition, the bionic antibacterial film also contains hyaluronic acid and gelatin besides epsilon-polylysine and chitosan. Hyaluronic acid is one of the important components of the extracellular matrix, and gelatin is a collagen hydrolysis product and has a function similar to collagen, so that the addition of hyaluronic acid and gelatin can enable the epsilon-polylysine bionic antibacterial membrane to be closer to the extracellular matrix in terms of components, has better affinity with skin, and is beneficial to adhesion, growth and migration of skin epithelial cells. In addition, the epsilon-polylysine bionic antibacterial film also has a patterned surface, so that the interfacial property of the dressing is further improved, skin epithelial cells around the wound surface can be better guided to crawl along the film, and the wound surface healing is further promoted. Therefore, the invention overcomes the defects of the prior art and plays an important role in the field of skin wound repair.
Disclosure of Invention
The invention discloses an epsilon-polylysine bionic antibacterial film.
The epsilon-polylysine bionic antibacterial film is prepared by the following specific technical scheme: preparing a gelling aqueous solution containing epsilon-polylysine, chitosan, hyaluronic acid, gelatin, polyvinyl alcohol and glycerol, adding a cross-linking agent to perform a cross-linking reaction in a patterned template to obtain gel, and neutralizing, cleaning and drying to obtain the epsilon-polylysine bionic antibacterial film.
The molecular weight of the epsilon-polylysine is 3000-6000kDa, and the concentration of the epsilon-polylysine in the gelling aqueous solution is 1-10% (w/v, g/ml);
the molecular weight of the chitosan is 5000-300000kDa, and the concentration of the chitosan in the gel-forming aqueous solution is 1-10% (w/v, g/ml);
the molecular weight of the hyaluronic acid is 1000000-4000000kDa, and the concentration of the hyaluronic acid in the gelling aqueous solution is 0.1-1% (w/v, g/ml).
The gelatin comprises one or two of alkaline gelatin and acidic gelatin;
the gelatin has a jelly strength of greater than 100Bloom g and a concentration in aqueous gelling solution of 3-15% (w/v, g/ml).
The polymerization degree of the polyvinyl alcohol is 2-5 ten thousand, and the concentration of the polyvinyl alcohol in the gelling aqueous solution is 1-5% (w/v, g/ml);
the volume ratio of the glycerol in the gelling aqueous solution is 1-10% (v/v);
the cross-linking agent is one of formaldehyde, glutaraldehyde, genipin and carbodiimide;
the concentration of the cross-linking agent after the cross-linking agent is mixed with the gel-forming aqueous solution is 0.01-3% (w/v, g/ml).
The patterning template is made of polytetrafluoroethylene;
the patterning template is a container with an opening at the upper end and a closed lower end, and the bottom surface of the container is provided with a required pattern formed by upward bulges and/or downward sunken grooves;
the width of the protrusion and the groove are 20-100 micrometers respectively, and the height of the protrusion and the depth of the groove are 20-100 micrometers respectively.
The crosslinking conditions are 18-25 ℃ of temperature and 50-80% of humidity.
The neutralization process is to soak the crosslinked gel with 0.1M glycine solution.
The drying condition is 18-25 deg.C and 20% humidity.
The bionic antibacterial epsilon-polylysine film comprises 2.2-62.1% (w/w), 0.2-6.2% (w/w), 6.6-93% (w/w), 2.2-31% (w/w), 2.2-62.1% (w/w) and 0.02-18.6% (w/w).
An application of an epsilon-polylysine bionic antibacterial film as a skin surface wound dressing.
THE ADVANTAGES OF THE PRESENT INVENTION
1. The epsilon-polylysine and the chitosan are compounded for use, so that the epsilon-polylysine bionic antibacterial membrane is prepared, and the antibacterial capability of the membrane is further improved;
2. in addition to epsilon-polylysine and chitosan, the biomimetic antibacterial film also contains hyaluronic acid and gelatin. Hyaluronic acid is one of the important components of the extracellular matrix, and gelatin is a collagen hydrolysis product and has a function similar to collagen, so that the addition of hyaluronic acid and gelatin can enable the epsilon-polylysine bionic antibacterial membrane to be closer to the extracellular matrix in composition, has better affinity with skin, and is beneficial to adhesion, growth and migration of skin epithelial cells;
3. the epsilon-polylysine bionic antibacterial membrane also has a cross-shaped patterned surface, so that the interface characteristic of the membrane is further improved, skin epithelial cells around the wound surface can be better guided to crawl along the membrane, and the wound surface healing is further promoted.
Drawings
Fig. 1 shows a cross pattern of trenches recessed downward on the bottom surface of a patterned template.
Detailed Description
Example 1:
an aqueous gelling solution containing 10% (w/v, g/ml) epsilon-polylysine (molecular weight 3000kDa), 1% (w/v, g/ml) chitosan (molecular weight 300000kDa), 1% (w/v, g/ml) hyaluronic acid (1000000kDa), 15% (w/v, g/ml) acidic gelatin (jelly strength 150Bloom g), 1% (w/v, g/ml) polyvinyl alcohol (degree of polymerization 2 ten thousand), 10% (v/v) glycerol was prepared. Then, a crosslinking agent glutaraldehyde was added to the gelling aqueous solution at a final concentration of 3% (w/v, g/ml), and a crosslinking reaction was performed in a patterned template (fig. 1 is a bottom surface pattern, a trench width is 40 micrometers, and a trench depth is 60 micrometers) to obtain a gel. The crosslinking conditions were a temperature of 23 ℃ and a humidity of 80%. Thereafter, the gel formed by crosslinking was soaked with a 0.1M glycine solution, neutralized, and washed with water. Then drying the mixture at the temperature of 20 ℃ and the humidity of 20 percent to obtain the epsilon-polylysine bionic antibacterial film. Two control groups were set: control 1 was a membrane (conventional chitosan membrane) without epsilon-polylysine, and the other conditions were consistent with the above conditions; control 2 was a film without hyaluronic acid and gelatin, and the other conditions were identical to those described above.
The prepared epsilon-polylysine bionic antibacterial film and the two control group films are cut into round sheets with the diameter of 1cm, the diameters of antibacterial rings of the bionic antibacterial film and the control group films to staphylococcus aureus are respectively counted by using an antibacterial ring method, and the antibacterial abilities of the bionic antibacterial film and the control group films are compared. In addition, rabbit skin abrasion model was prepared, and the same shape and size (3X 3 cm)2) The epsilon-polylysine bionic antibacterial membrane and the two control group membranes are attached to the same area (4 cm)2) And on the wound surface of the injury degree, periodically sampling and observingThe time to skin healing was observed and recorded.
Experiments show that the diameters of the inhibition zones of the epsilon-polylysine bionic antibacterial membrane, the control group 1 membrane and the control group 2 membrane on staphylococcus aureus are 8.2cm, 2.3cm and 7.8cm respectively, which indicates that the bionic antibacterial membrane added with 10% (w/v, g/ml) epsilon-polylysine has better antibacterial ability than the conventional chitosan membrane without epsilon-polylysine; the antibacterial ability of the epsilon-polylysine bionic antibacterial film is not obviously influenced by not adding hyaluronic acid and gelatin. In addition, animal model experiments show that the healing time of skin wound surfaces of the epsilon-polylysine bionic antibacterial membrane, the control group 1 membrane and the control group 2 membrane is 8 days, 10 days and 15 days respectively, which indicates that the bionic antibacterial membrane added with 10% (w/v, g/ml) epsilon-polylysine and the conventional chitosan membrane not added with epsilon-polylysine both contain hyaluronic acid and gelatin, so that the bionic antibacterial membrane has the same capacity of supporting epithelial cell fusion, and the healing of the wound surfaces of the membranes not added with hyaluronic acid and gelatin is obviously slowed down.
Example 2:
an aqueous gelling solution containing 1% (w/v, g/ml) epsilon-polylysine (molecular weight 6000kDa), 10% (w/v, g/ml) chitosan (molecular weight 5000kDa), 0.1% (w/v, g/ml) hyaluronic acid (4000000kDa), 3% (w/v, g/ml) basic gelatin (jelly strength 220Bloom g), 5% (w/v, g/ml) polyvinyl alcohol (degree of polymerization 5 ten thousand), 1% (v/v) glycerol was prepared. Thereafter, glutaraldehyde, a crosslinking agent, was added to the gelling aqueous solution to a final concentration of 0.01% (w/v, g/ml), and a crosslinking reaction was performed in the patterned template (fig. 1, same as example 1) to obtain a gel. The crosslinking conditions were a temperature of 25 ℃ and a humidity of 50%. Thereafter, the gel formed by crosslinking was soaked with a 0.1M glycine solution, neutralized, and washed with water. Then drying the mixture at the temperature of 22 ℃ and the humidity of 20 percent to obtain the epsilon-polylysine bionic antibacterial film. 4 control groups were set:
control 1 was a membrane (conventional chitosan membrane) without epsilon-polylysine, and the other conditions were consistent with the above conditions;
control 2 was a membrane supplemented with 0.8% (w/v, g/ml) epsilon-polylysine, and the other conditions were identical to the above conditions;
control 3 was a membrane supplemented with 10% (w/v, g/ml) ε -polylysine, and the other conditions were identical to those described above.
Control 4 was a membrane supplemented with 12% (w/v, g/ml) ε -polylysine, and the other conditions were identical to those described above.
The prepared epsilon-polylysine bionic antibacterial film and 4 control group films are cut into round pieces with the diameter of 1cm, the diameters of antibacterial rings of the bionic antibacterial film and the control group films on gram-negative bacteria are respectively counted by using an antibacterial ring method, and the antibacterial activities of the bionic antibacterial films and the control group films are compared.
Experiments show that the diameters of inhibition zones of the epsilon-polylysine bionic antibacterial film and the control groups 1-4 for gram-negative bacteria are 7.7cm, 4.1cm, 4.5cm, 10.5cm and 5.2cm respectively. The results show that: 1) the bionic antibacterial film added with 0.8% (w/v, g/ml) epsilon-polylysine and the conventional chitosan film have the same lower antibacterial ability, and are both lower than the bionic antibacterial film added with 1% (w/v, g/ml) epsilon-polylysine; 2) the bionic antibacterial film added with 10% (w/v, g/ml) of epsilon-polylysine has the highest antibacterial ability, which shows that the antibacterial ability of the film is enhanced along with the increase of the concentration of the epsilon-polylysine in a certain range; 3) the concentration of the epsilon-polylysine is further improved, but the bacteriostatic ability is obviously reduced, namely the bacteriostatic ability of the bionic bacteriostatic film added with 12 percent (w/v, g/ml) of the epsilon-polylysine is even lower than that of the bionic bacteriostatic film added with 1 percent (w/v, g/ml) of the epsilon-polylysine, because the concentration of the epsilon-polylysine is too high, the epsilon-polylysine has obvious combination effect with the acidic polysaccharide, and the bacteriostatic ability of the film is obviously reduced. Therefore, the preparation conditions of the membrane with the optimal bacteriostatic ability are as follows: the concentration of the epsilon-polylysine in the gelling water solution is 1-10% (w/v, g/ml), namely the mass percent of the epsilon-polylysine in the dried film is 2.2-62.1% (w/w).
Claims (7)
1. A preparation method of an epsilon-polylysine bionic antibacterial film is characterized by comprising the following steps: preparing a gelling aqueous solution containing epsilon-polylysine, chitosan, hyaluronic acid, gelatin, polyvinyl alcohol and glycerol, adding a cross-linking agent to perform a cross-linking reaction in a patterned template to obtain gel, and neutralizing, cleaning and drying to obtain the epsilon-polylysine bionic antibacterial film;
the weight volume concentration of the epsilon-polylysine in the gelling aqueous solution is 1-10 percent g/ml;
the weight volume concentration of the chitosan in the gelling aqueous solution is 1-10% g/ml;
the weight volume concentration of the hyaluronic acid in the gelling water solution is 0.1-1% g/ml;
the weight volume concentration of the gelatin in the gelling aqueous solution is 3-15% g/ml;
the weight volume concentration of the polyvinyl alcohol in the gelling aqueous solution is 1-5% g/ml;
the volume ratio of the glycerol in the gelling aqueous solution is 1-10%;
the molecular weight of the epsilon-polylysine is 3000-6000 Da;
the molecular weight of the chitosan is 5000-;
the molecular weight of the hyaluronic acid is 1000000-4000000 Da;
the gelatin has a jelly strength of greater than 100Bloom g;
the polymerization degree of the polyvinyl alcohol is 2-5 ten thousand;
the weight volume concentration of the cross-linking agent after the cross-linking agent is mixed with the gelling aqueous solution is 0.01-3% g/ml;
the crosslinking conditions are 18-25 ℃ of temperature and 50-80% of humidity.
2. The method of claim 1, wherein:
the gelatin comprises one or two of alkaline gelatin and acidic gelatin.
3. The method of claim 1, wherein:
the cross-linking agent is one of formaldehyde, glutaraldehyde, genipin and carbodiimide.
4. The method of claim 1, wherein:
the patterning template is made of polytetrafluoroethylene;
the patterning template is a container with an opening at the upper end and a closed lower end, and the bottom surface of the container is provided with a required pattern formed by upward bulges and/or downward sunken grooves;
the width of the protrusion and the groove are 20-100 micrometers respectively, and the height of the protrusion and the depth of the groove are 20-100 micrometers respectively.
5. The method of claim 1, wherein:
the neutralization process is to soak the crosslinked gel with 0.1M glycine solution.
6. The method of claim 1, wherein:
the drying condition is 18-25 deg.C and 20% humidity.
7. An epsilon-polylysine biomimetic antibacterial film prepared by the method of any of claims 1-6.
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| CN109021272B (en) * | 2018-06-07 | 2020-12-04 | 宁夏金博乐食品科技有限公司 | Edible gelatin-based film and preparation method thereof |
| CN109613071B (en) * | 2019-01-11 | 2021-07-27 | 电子科技大学 | Humidity sensor of humidity-sensitive composite membrane based on polylysine modified carbon-based material and preparation method thereof |
| CN110507845A (en) * | 2019-09-25 | 2019-11-29 | 广州沁瀚生物科技有限公司 | Biological composite ventilating dressing and preparation method thereof |
| CN112826983A (en) * | 2019-11-22 | 2021-05-25 | 中国科学院大连化学物理研究所 | Heart acellular matrix modified bionic membrane and preparation and application thereof |
| CN111068102B (en) * | 2019-12-18 | 2022-04-12 | 陕西科技大学 | Enzyme-catalyzed blood-coagulation antibacterial controllable gelatin-based hydrogel and preparation method thereof |
| CN111363171B (en) * | 2020-04-02 | 2022-10-25 | 南昌大学第二附属医院 | Antibacterial hydrogel and preparation method and application thereof |
| CN111358940A (en) * | 2020-05-14 | 2020-07-03 | 深圳爱思得美容科技有限公司 | Application of scar removing, preventing and controlling combined liquid |
| CN114984300B (en) * | 2022-05-30 | 2023-08-01 | 浙江大学 | Tough antibacterial hydrogel dressing and preparation method thereof |
| CN115501376B (en) * | 2022-09-16 | 2023-09-15 | 常州美杰医疗用品有限公司 | Gel type antibacterial medical band-aid and preparation method thereof |
| CN115887748B (en) * | 2022-12-13 | 2023-09-01 | 海南鸿翼医疗器械有限公司 | Medical functional dressing for inhibiting bacteria and promoting healing and preparation method thereof |
| CN117603577A (en) * | 2023-11-28 | 2024-02-27 | 上海市第四人民医院 | Photo-crosslinking hydrogel and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178974A (en) * | 2010-01-15 | 2011-09-14 | 东华大学 | Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application |
| CN105854090A (en) * | 2016-04-11 | 2016-08-17 | 大连诺伊生物技术有限责任公司 | Quick release-slow release drug membrane and preparation method thereof |
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| KR101777910B1 (en) * | 2015-04-30 | 2017-09-13 | 주식회사 제네웰 | A composition for wound healing, method of producing the same and dressing using the same |
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| CN102178974A (en) * | 2010-01-15 | 2011-09-14 | 东华大学 | Bacterial cellulose-based antibacterial dry film for acute trauma and preparation method and application |
| CN105854090A (en) * | 2016-04-11 | 2016-08-17 | 大连诺伊生物技术有限责任公司 | Quick release-slow release drug membrane and preparation method thereof |
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