CN108060190A - A kind of method using edible fungus bran production monose - Google Patents
A kind of method using edible fungus bran production monose Download PDFInfo
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- CN108060190A CN108060190A CN201711330644.7A CN201711330644A CN108060190A CN 108060190 A CN108060190 A CN 108060190A CN 201711330644 A CN201711330644 A CN 201711330644A CN 108060190 A CN108060190 A CN 108060190A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
- C08H6/00—Macromolecular compounds derived from lignin, e.g. tannins, humic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/02—Glucose; Glucose-containing syrups obtained by saccharification of cellulosic materials
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- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K13/00—Sugars not otherwise provided for in this class
- C13K13/002—Xylose
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Abstract
The present invention relates to edible fungus bran exploitation technical field, further to a kind of method using edible fungus bran production monose.Overcome hemicellulose level existing in the prior art low, xylose and glucose is difficult to separate, it is difficult to the problem of carrying out industrialized production.Present invention the step of using for：Step 1: sour water solution：Edible fungus bran is air-dried and crushed, by solid-to-liquid ratio 1：10~20 add in the dilute acid soln that mass concentration is 1 5%, and 100 120 DEG C of 30 120min of processing obtain filtrate and filter residue after filtering, xylose prepares food-grade xylose in filtrate；Step 2: basic hydrolysis：Filter residue presses 1：10~20 solid-to-liquid ratios add in the aqueous slkali that mass concentration is 1 5%, after 40 60 DEG C handle 60 120min, are neutralized to neutrality with acid, measure content of cellulose；Step 3: enzyme hydrolysis：Cellulase is added in by content of cellulose, and cellulose dosage is 10 20FPU/g celluloses, 50 DEG C, obtain enzymolysis liquid when enzymolysis 48 72 is small under the conditions of 180rpm, filters to obtain glucose solution and lignin.
Description
Technical field：
The present invention relates to edible fungus bran exploitation technical fields, are given birth to further to a kind of using edible fungus bran
The method for producing monose.
Technical background：
Edible fungus bran (is given up in paper mill with corncob, sawdust, cotton seed hulls, straw, agricultural crop straw or industrial waste
Liquid, vinegar grain, vinasse etc.) as the solid medium discarded after primary raw material substituting stuff cultivation edible mushroom.Current edible mushroom is total in China
Yield is up to 400~5,000,000 tons, 30,000,000,000 yuan of output value or more.Resulting mushroom bran quantity is also very huge, and annual output reaches
700~9,000,000 tons.Low to the Application way overall utilization of mushroom bran at present, added value is not high, has both caused substantial amounts of resource
Waste, and pollution is caused to environment.With the fast development of mushroom industry, how more efficiently processing is edible discarded
Mushroom bran becomes one of edible mushroom industry important topic urgently to be resolved hurrily.
After mushroom bran is utilized by edible fungi growth, wherein substantial amounts of cellulose, hemicellulose and lignin are still rich in,
According to the difference of matrix, crude fiber content accounts for 10-30% in mushroom bran, and crude protein content 6-13% [edible and medical fungi, 2015,23
(2)：72‐75].To the research on utilization of this part of renewable resource, fertilizer and feed aspect are concentrated mainly at present.As directly
It connects using mushroom bran as absorption carrier microbe inoculation fertilizer, such as patent 201510748273.9, this method is just with bacterium
The sponginess of chaff structure is adsorbed as carrier, and the nutritional ingredient of itself is not utilized, utilization rate and added value
It is not high；Or certain compost fermentation is carried out to mushroom bran and is handled, the macromolecular substances in mushroom bran is made to change into small-molecule substance
Afterwards, inoculate microbial bacterial agent, prepare microbial manure, such as patent CN 105777231A, in this kind of method mushroom bran pass through from
Right fermentation maturity, macromolecular substances are degraded and utilized, and improve utilization rate, but still there are the spontaneous fermentation cycle is long,
The shortcomings that taking up a large area.The glucose and xylose generated in mushroom bran after substantial amounts of cellulose and hydrolysis of hemicellulose is all can
The monose utilized, wherein xylose can be further converted into the industrial products such as xylitol, furfural；Glucose can be used as fermentation
Carbon source produces various fermented products, and the cellulose in mushroom bran and hemicellulose are changed into monose, then further developed
It utilizes, is a kind of effective transform mode for improving mushroom bran added value.But due in natural wooden fiber's element structure
Cellulose and hemicellulose are wrapped in inside lignin, it is necessary to can be just applied by certain pretreatment.Patent
200910086798.5, using the lignin in basic hydrolysis removing mushroom bran, make cellulose and half with the method for enzyme hydrolysis again afterwards
Cellulose is converted into glucose and xylose, improves the whole utilization degree of mushroom bran, but alkali process is while lignin is removed,
Can hydrolyzed hemicellulose, lose hemicellulose, while remaining the hemicellulose xylose that generates and cellulose water in enzymolysis
The glucose that solution generates mixes, and the property of two kinds of monose approaches, and conventional isolation techniques are difficult to be separated.Therefore again
Using being limited, industrialized production is difficult to realize.
The content of the invention：
The present invention will provide a kind of method using edible fungus bran production monose, to overcome existing in the prior art half
Content of cellulose is low, and xylose and glucose is difficult to separate, it is difficult to the problem of carrying out industrialized production.
In order to reach the purpose of the present invention, technical solution provided by the invention is：It is a kind of to be given birth to using edible fungus bran
The method for producing monose, comprises the following steps：
Step 1: sour water solution：Edible fungus bran is air-dried and crushed, by solid-to-liquid ratio 1：10~20 add in mass concentration as 1-
5% dilute acid soln, 100-120 DEG C of processing 30-120min, obtains filtrate and filter residue after filtering, xylose prepares food-grade in filtrate
Xylose；
Step 2: basic hydrolysis：Filter residue presses 1：10~20 solid-to-liquid ratios add in the aqueous slkali that mass concentration is 1-5%, 40-60
After DEG C processing 60-120min, it is neutralized to neutrality with acid, measures content of cellulose；
Step 3: enzyme hydrolysis：Cellulase is added in by content of cellulose, cellulose dosage is 10-20FPU/g celluloses,
50 DEG C, digest under the conditions of 180rpm 48-72 it is small when obtain enzymolysis liquid, filter to obtain glucose solution and lignin.
Dilute acid soln is sulfuric acid or hydrochloric acid in above-mentioned steps one, in above-mentioned filtrate xylose by neutralizing, concentration, decoloration, from
Son exchanges and crystallization prepares food-grade xylose.
Two neutral and alkali solution of above-mentioned steps is sodium hydroxide or calcium hydroxide solution.
Compared with prior art, it is an advantage of the invention that：
1st, it can realize that the complete alternation of mushroom bran uses, realize zero-emission：This patent using soda acid coupling technique to mushroom bran into
Row hydrolysis process, the xylose and glucose point for being generated the hemicellulose in mushroom bran and cellulose hydrolysis using two-step process
Step is collected, and is utilized step by step.The first step selects suitable dilute acid hydrolysis condition that the hemicellulose in mushroom bran is made to obtain to the greatest extent
Hydrolysis, avoids loss of the hemicellulose in subsequent alkaline processing procedure, so that the yield of xylose maximizes；Second step is adopted
Acidolysis raw material is handled with diluted alkaline, is destroyed between ester bond and ether between lignin, removes partial lignin, improves the enzyme of cellulose
Solution rate, the cellulosic material after alkali process generate glucose after cellulase degradation.Solid residue after enzymolysis is main
It is modified to be used for producing lignin derivative for lignin.
2nd, for the present invention by carrying out soda acid coupling pretreatment to mushroom bran, used process conditions are mild, cellulose and half
Cellulose loss is small, can produce xylose, glucose and lignin simultaneously, realizes point of cellulose, hemicellulose and lignin
Grade utilizes, and improves its added value；Its recycling is made to become simple possible, it can be achieved that large-scale industrial production so that edible
The efficient process of waste mushroom leftover becomes a reality.
3rd, environmental pollution is small：In the preparation process of the present invention, directly neutralized using diluted alkaline after dilute acid hydrolysis, dropped significantly
The discharge of low acid, reduces the pollution to environment.
Description of the drawings：
Fig. 1 is the process flow chart of the present invention.
Specific embodiment：
The present invention provides a kind of method using edible fungus bran production monose, specially utilizes the side of two-step method
Method fractional hydrolysis hemicellulose and cellulose obtain the method for xylose and glucose.
The present invention will be described in detail by specific embodiment below.
Embodiment 1：
Step 1, sour water solution：It flat will eat, the various edible fungus brans such as coprinus comatus, acupuncture needle are eaten, 10 mesh be crushed to after air-drying
Left and right, by solid-to-liquid ratio 1：10 add in mass concentrations be 5% sulfuric acid solution in, 100 DEG C processing 30min, after filtering filtrate and
Melt cinder；
The content of xylose in filtrate is measured as 13g/L or so, the conversion ratio of hemicellulose can reach more than 92%.Filtrate
By neutralizing, concentrating, decolourizing, ion exchange and crystallization prepare food-grade xylose, the yield of xylose can reach 100g/kg mushroom brans,
It can also further produce the products such as xylitol and furfural.
Step 2, basic hydrolysis：Filter residue presses 1：10 solid-to-liquid ratios are added in the sodium hydroxide solution that mass concentration is 5%, 60 DEG C
After handling 60min, neutrality is neutralized to acid, measures the content of cellulose；
Step 3, enzyme hydrolysis：By content of cellulose add in commercial fibre element enzyme (CTec2, Novi of Denmark letter enzyme
Preparation company produces), dosage is 20FPU/g celluloses, 50 DEG C, obtain enzymolysis liquid, enzymolysis liquid when enzymolysis 48 is small under the conditions of 180rpm
Filtrate and filter residue are obtained after filtering, and filtrate is glucose solution, and yield is after measured up to 40g/L enzymolysis liquids, after dilution
It can be used as cultivating the carbon source of various microorganisms；Filter residue main component is lignin, and various wood can be produced by modification
Quality derivative.
Embodiment 2：
Step 1, sour water solution：It flat will eat, the various edible fungus brans such as coprinus comatus, acupuncture needle are eaten, 10 mesh be crushed to after air-drying
Left and right, by solid-to-liquid ratio 1：15 add in mass concentrations be 2% sulfuric acid solution in, 120 DEG C processing 60min, after filtering filtrate and
Melt cinder；The content of xylose in filtrate is measured as 9g/L or so, the conversion ratio of hemicellulose can reach 90%.The neutralization of filtrate process,
Concentration, decoloration, ion exchange and crystallization prepare food-grade xylose, and the yield of xylose can reach 98g/kg mushroom brans.
Step 2, basic hydrolysis：Filter residue presses 1：15 solid-to-liquid ratios are added in the sodium hydroxide solution that mass concentration is 2%, 60 DEG C
100min is handled, neutrality is neutralized to acid, measures the content of cellulose；
Step 3, enzyme hydrolysis：By content of cellulose add in commercial fibre element enzyme (CTec2, Novi of Denmark letter enzyme
Preparation company produces), dosage is 10FPU/g celluloses, 50 DEG C, obtain enzymolysis liquid, enzymolysis liquid when enzymolysis 60 is small under the conditions of 180rpm
Filtrate and filter residue are obtained after filtering, and filtrate is glucose solution, and yield is after measured up to 25g/L enzymolysis liquids.
Embodiment 3：
Step 1, sour water solution：It flat will eat, the various edible fungus brans such as coprinus comatus, acupuncture needle are eaten, 10 mesh be crushed to after air-drying
Left and right, by solid-to-liquid ratio 1：20 add in the sulfuric acid solution that mass concentration is 5%, and 100 DEG C of processing 120min obtain filtrate after filtering
And melt cinder；
The content of xylose in filtrate is measured as 7g/L or so, the conversion ratio of hemicellulose can reach 89%.During filtrate is passed through
Food-grade xylose is prepared with concentration, decoloration, ion exchange and crystallization, the yield of xylose can reach 97g/kg mushroom brans.
Step 2, basic hydrolysis：Filter residue presses 1：20 solid-to-liquid ratios are added in the sodium hydroxide solution that mass concentration is 5%, 60 DEG C
After handling 60min, neutrality is neutralized to acid, measures the content of cellulose；
Step 3, enzyme hydrolysis：By content of cellulose add in commercial fibre element enzyme (CTec2, Novi of Denmark letter enzyme
Preparation company produces), dosage is 15FPU/g celluloses, 50 DEG C, obtain enzymolysis liquid, enzymolysis liquid when enzymolysis 72 is small under the conditions of 180rpm
Filtrate and filter residue are obtained after filtering, and filtrate is glucose solution, and yield is after measured up to 18g/L enzymolysis liquids.
Above-described embodiment 1 is most preferred embodiment.
Claims (3)
- A kind of 1. method using edible fungus bran production monose, it is characterised in that：Comprise the following steps：Step 1: sour water solution：Edible fungus bran is air-dried and crushed, by solid-to-liquid ratio 1：10~20 add in mass concentration as the dilute of 1-5% Acid solution, 100-120 DEG C of processing 30-120min, obtains filtrate and filter residue after filtering, xylose prepares food-grade xylose in filtrate；Step 2: basic hydrolysis：Filter residue presses 1：10~20 solid-to-liquid ratios add in the aqueous slkali that mass concentration is 1-5%, 40-60 DEG C of processing After 60-120min, neutrality is neutralized to acid, measures content of cellulose；Step 3: enzyme hydrolysis：By content of cellulose add in cellulase, cellulose dosage be 10-20FPU/g celluloses, 50 DEG C, Enzymolysis liquid is obtained when enzymolysis 48-72 is small under the conditions of 180rpm, filters to obtain glucose solution and lignin.
- 2. a kind of method using edible fungus bran production monose according to claim 1, it is characterised in that：Above-mentioned steps Dilute acid soln is sulfuric acid or hydrochloric acid in one, in above-mentioned filtrate xylose by neutralizing, it is prepared by concentration, decoloration, ion exchange and crystallization Food-grade xylose.
- 3. a kind of method using edible fungus bran production monose according to claim 1 or 2, it is characterised in that：It is above-mentioned Step 2 neutral and alkali solution is sodium hydroxide or calcium hydroxide solution.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110204381A (en) * | 2019-07-15 | 2019-09-06 | 绥化学院 | A kind of method that modified auricuralia auricular bran prepares biological organic complex fertilizer |
| CN110714041A (en) * | 2019-11-19 | 2020-01-21 | 云南省烟草农业科学研究院 | Method for efficiently and directly producing glucose by using waste tobacco stems |
-
2017
- 2017-12-13 CN CN201711330644.7A patent/CN108060190A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110204381A (en) * | 2019-07-15 | 2019-09-06 | 绥化学院 | A kind of method that modified auricuralia auricular bran prepares biological organic complex fertilizer |
| CN110714041A (en) * | 2019-11-19 | 2020-01-21 | 云南省烟草农业科学研究院 | Method for efficiently and directly producing glucose by using waste tobacco stems |
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