CN104232494A - Method for producing saccharomycete and organic fertilizer mother solution by utilizing papermaking black liquor - Google Patents
Method for producing saccharomycete and organic fertilizer mother solution by utilizing papermaking black liquor Download PDFInfo
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- CN104232494A CN104232494A CN201410444780.9A CN201410444780A CN104232494A CN 104232494 A CN104232494 A CN 104232494A CN 201410444780 A CN201410444780 A CN 201410444780A CN 104232494 A CN104232494 A CN 104232494A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
The invention discloses a method for producing a saccharomycete and an organic fertilizer mother solution by utilizing papermaking black liquor. The method is characterized by comprising the following steps of carrying out aeration pretreatment on papermaking digesting black liquor, and then regulating a pH value to 4-7; culturing candida utilis to obtain fermenting strain liquid; carrying out second-stage strain culture on the saccharomycete by utilizing diluted digesting black liquor and nutrient substances; adding a second-stage seed to the pretreated papermaking black liquor for fermentation; and after the fermentation is completed, filtering to obtain the saccharomycete; filtering a supernatant, and carrying out spraying granulation or concentration compounding to obtain an organic fertilizer. The method disclosed by the invention extends the treatment modes of the papermaking black liquor, effectively reduces the BOD (Biochemical Oxygen Demand) of black liquor, effectively realizes the recycling of the papermaking black liquor, reduces the sewage treatment cost and is simple in process and high in practicability. The saccharomycete can be used for producing ribonucleic acid and single-cell protein, and the organic fertilizer can increase the crop yield and enhance the stress resistance of crops because of rich fulvic acid and is an environmental-friendly fertilizer suitable for modern agriculture.
Description
Technical field
The present invention relates to technical field of sewage, particularly relate to a kind of method utilizing black liquid to produce yeast and fertilizer.
Background technology
The above papermaking in county of China and county and paper products industry wastewater discharge account for 18.6% of national industrial total release, wherein process emission compliance amount and only account for 49.3% of paper-making industrial waste water total release, find that pollutent contained in pulping cooking waste liquor (common name black liquid) has accounted for more than 90% of disposal of pollutants total amount through test.Therefore, the main contributor that black liquid discharge is polluted as paper mill, is badly in need of the method working out process black liquid, avoids it to environment.Find in the analysis to black liquor of pulp making composition, black liquor contains the organism such as a large amount of xanthohumic acids, hemicellulose and cellulosic resolvent, make the COD of waste water up to 100g/L, BOD also has 50g/L, as chemically processed, complex process, needs the input of a large amount of chemical reagent and equipment, and cost is too high.Xanthohumic acid, micromolecular polysaccharide are effective nutritive ingredients that directly can be absorbed by plant, therefore, are utilized by black liquor and development as resource and receive increasing concern.
Hemicellulose in stalk is the heteromultimer that five-carbon sugar and hexose are formed, in acid conditions of cooking, be easily degraded to monose or polysaccharide.Candida utilis, its protein, vitamins B and rna content enrich, and can make full use of five-carbon sugar and hexose growth and breeding using urea and nitric acid in the substratum of nitrogenous source.
Summary of the invention
The invention discloses a kind of method utilizing black liquid to produce yeast and fertilizer mother liquor, the method is that a kind of chemistry and biotechnology combine the technological method of Processing Paper Wastewater, not only operation is simple, processing cost is low, non-secondary pollution, and the recycling effectively achieving black liquid.
Utilize black liquid to produce a method for yeast and fertilizer mother liquor, mainly comprise following processing step:
(1) black liquid pre-treatment: black liquor is crossed and filtered insoluble substance, gas explosion process 30min ~ 120min, adds diluted acid or diluted alkaline regulates black liquor pH to 4 ~ 7, redilution 0.1 ~ 5 times;
(2) cultivation of Ruan candidiasis bacterial classification is produced: containing glucose 19g/L, yeast extract paste 9g/L, K2HPO4 1.5 substratum of g/L and MgSO4 1.2g/L, pH value 4 ~ 7, leavening temperature 20 DEG C ~ 35 DEG C, cultivates 8h ~ 36h under rotating speed 180rpm condition;
(3) bacterial classification secondary enlarged culturing: get step (1) gained black liquor and again dilute 0.1 ~ 10 times, the urea adding 0.01% ~ 5% is nitrogenous source, ratio in 0.1% ~ 10% adds gained bacterial classification in step (2), in pH value 4 ~ 7, leavening temperature 20 DEG C ~ 35 DEG C, cultivates 8h ~ 36h under rotating speed 180rpm condition;
(4) black liquor fermentation: add gained strain liquid in step (3) in the ratio of 0.1% ~ 10% in step (1) gained process black liquor, in pH value 4 ~ 7, leavening temperature 20 DEG C ~ 35 DEG C, cultivates 8h ~ 36h under rotating speed 180rpm condition;
(5) thalline obtains: carried out filtering or centrifugal treating by step (4) gained fermented liquid, obtains yeast thalline and ferment filtrate;
(6) fertilizer mother liquor preparation: step (5) gained ferment filtrate is concentrated into dry-matter final concentration 30% ~ 50%, obtains fertilizer mother liquor.
For technique scheme, the diluted acid described in step (1) is hydrochloric acid or sulfuric acid or phosphoric acid, and described diluted alkaline is sodium hydroxide or potassium hydroxide or sodium carbonate or salt of wormwood;
In described yeast thalline extractive technique, by fermented liquid conventional filtration or centrifugal method, obtain yeast thalline;
Utilize gained fertilizer mother liquor in described step (6) to prepare the method for fertilizer, its method is as follows:
(1) with the fertilizer mother liquor of gained be base fluid and urea, at least one in potassium sulfate, potassium primary phosphate, trace element is composite, make fertilizer, each composition weight proportioning is: fertilizer mother liquor 10% ~ 80%, urea 5% ~ 30%, potassium sulfate 5% ~ 20%, trace element 2% ~ 20%, potassium primary phosphate 5% ~ 20%, trace element is selected from copper, iron, manganese, zinc, boron, molybdenum;
(2) by fertilizer mother liquor and urea, potassium sulfate, potassium primary phosphate, trace element built, namely abundant reaction obtains high-efficiency foliage fertilizer, and weight proportion is as follows: fertilizer mother liquor 10% ~ 70%, urea 5% ~ 25%, potassium sulfate 5% ~ 18%, trace element 2% ~ 10%, potassium primary phosphate 5% ~ 15%;
(3) by fertilizer mother liquor and urea, potassium sulfate, potassium primary phosphate trace element built, abundant mixing, granulation, drying, namely sieve obtains black liquid fertilizer, weight proportion is as follows: fertilizer mother liquor 10% ~ 70%, urea 5% ~ 25%, potassium sulfate 5% ~ 18%, trace element 2% ~ 10%, potassium primary phosphate 5% ~ 15%.
This technology utilizes black liquid to cultivate and obtains producing Ruan's candiyeast, while effectively reducing black liquor BOD, the thalline obtained can in order to extract Yeast Nucleic Acid, produce seasonings, carcinostatic agent or the antiviral agent such as inosine monophosphate and guanosine monophosphate ester, also can directly protein be used in fodder production.What the fertilizer product that the method obtains contained enriches xanthohumic acid, can adjust by Promoting plant growth, improves crop anti-adversity ability, increase production and improve quality of agricultural product.
The method that the present invention utilizes chemistry and biotechnology to combine is by the recycling of black liquid, and its operation is simple, and processing cost is low, non-secondary pollution, and effectively achieves the recycling of black liquid.
Embodiment
Below in conjunction with following examples, the invention will be further described.
embodiment 1
Utilize black liquid to produce a method for yeast and fertilizer, it mainly comprises following processing step:
(1) black liquid pre-treatment: alkaline process papermaking is produced black liquor and filters, removing insoluble substance, gas explosion process 30min, adds diluted acid and is adjusted to black liquor pH to 5, redilution 0.1 times;
(2) cultivation of Ruan candidiasis bacterial classification is produced: containing glucose 19 g/L, yeast extract paste 9g/L, K
2hPO
41.5 g/L, MgSO
4in the substratum of 1.2g/L, pH value 5, leavening temperature 25 DEG C, cultivates 24h under rotating speed 180rpm condition;
(3) bacterial classification secondary enlarged culturing: get step (1) gained black liquor dilution 0.1 ~ 10 times, the urea adding 1% is nitrogenous source, ratio in 0.1% ~ 10% adds gained bacterial classification in step (2), in pH value 5, leavening temperature 25 DEG C, cultivates 8h ~ 36h under rotating speed 180rpm condition;
(4) black liquor fermentation: add the middle gained strain liquid of step (3) in pH value 6 in the ratio of 2% in step (1) gained process black liquor, leavening temperature 25 DEG C, cultivates 24h under rotating speed 180rpm condition;
(5) thalline obtains: filtration step (4) gained fermented liquid, obtains yeast thalline and ferment filtrate;
(6) preparation of fertilizer mother liquor: step (5) gained filtrate is concentrated into dry-matter final concentration 35%, obtains fertilizer mother liquor;
(7) preparation of fertilizer foliage fertilizer: will filter or the fermented supernatant fluid liquid of centrifugal rear gained and urea, potassium sulfate, potassium primary phosphate, trace element built, namely abundant reaction obtains high-efficiency foliage fertilizer, weight proportion is as follows: fertilizer mother liquor 70%, urea 5%, potassium sulfate 15%, trace element 5%, potassium primary phosphate 5%.
embodiment 2
A kind of concrete steps utilizing black liquid to produce yeast and fertilizer are:
(1) black liquid pre-treatment: sulphite slurrying gained black liquor is filtered, removing does not allow material, gas explosion process 120min, and rare potassium hydroxide is adjusted to black liquor pH to 7, redilution 5 times;
(2) produce the cultivation of Ruan candidiasis bacterial classification: containing glucose 19 g/L, yeast extract paste 9g/L, K2HPO4 1.5 substratum of g/L, MgSO4 1.2g/L, in pH value 7, leavening temperature 20 DEG C, cultivates 16h under rotating speed 180rpm condition;
(3) bacterial classification secondary enlarged culturing: get step (1) gained black liquor and dilute 5 times, the urea adding 5% is nitrogenous source, and the ratio in 10% adds gained bacterial classification in step (2), in pH value 7, leavening temperature 20 DEG C, cultivates 36h under rotating speed 180rpm condition;
(4) black liquor fermentation: add gained strain liquid in step (3) in the ratio of 10% in step (1) gained process black liquor, be 7 in pH value, leavening temperature 20 DEG C, cultivates 36h under rotating speed 180rpm condition;
(5) thalline obtains: filtration step (4) gained fermented liquid, obtains yeast thalline and ferment filtrate;
(6) preparation of fertilizer mother liquor: step (5) gained filtrate is concentrated into dry-matter final concentration 30%, obtains fertilizer mother liquor;
(7) with the fertilizer mother liquor of gained for base fluid and urea, potassium primary phosphate, trace element built, make fertilizer, each composition weight proportioning is: fertilizer mother liquor 80%, urea 5%, trace element 5%, potassium primary phosphate 10%, trace element is selected from copper, iron, manganese, zinc, boron, molybdenum.Trace element in above-described embodiment is the inner complex adopting copper iron, manganese, zinc, boron, the Sino-German several element of molybdenum.
embodiment 2
A kind of concrete steps utilizing black liquid to produce yeast and fertilizer are:
(1) black liquid pre-treatment: sulphite slurrying gained black liquor is filtered, removing does not allow material, gas explosion process 120min, and rare potassium hydroxide is adjusted to black liquor pH to 5, redilution 1 times;
(2) produce the cultivation of Ruan candidiasis bacterial classification: containing glucose 19 g/L, yeast extract paste 9g/L, K2HPO4 1.5 substratum of g/L, MgSO4 1.2g/L, in pH value 6, leavening temperature 25 DEG C, cultivates 30h under rotating speed 180rpm condition;
(3) bacterial classification secondary enlarged culturing: get step (1) gained black liquor and dilute 10 times, the urea adding 1% is nitrogenous source, and the ratio in 3% adds gained bacterial classification in step (2), in pH value 5, leavening temperature 25 DEG C, cultivates 12h under rotating speed 180rpm condition;
(4) black liquor fermentation: add gained strain liquid in step (3) in the ratio of 3% in step (1) gained process black liquor, be 6 in pH value, leavening temperature 25 DEG C, cultivates 12h under rotating speed 180rpm condition;
(5) thalline obtains: filtration step (4) gained fermented liquid, obtains yeast thalline and ferment filtrate;
(6) preparation of fertilizer mother liquor: step (5) gained filtrate is concentrated into dry-matter final concentration 40%, obtains fertilizer mother liquor;
(7) by fertilizer mother liquor and urea, potassium sulfate, potassium primary phosphate, trace element built, abundant mixing, granulation, drying, namely sieve obtains black liquid fertilizer, weight proportion is as follows: fertilizer mother liquor 70%, urea 10%, potassium sulfate 15%, trace element 5%, potassium primary phosphate 10%.
Trace element in above-described embodiment is the inner complex adopting copper iron, manganese, zinc, boron, the Sino-German several element of molybdenum.
embodiment 3
A kind of concrete steps utilizing black liquid to produce yeast and fertilizer are:
(1) black liquid pre-treatment: sulphite slurrying gained black liquor is filtered, removing does not allow material, gas explosion process 120min, and rare potassium hydroxide is adjusted to black liquor pH to 4, redilution 3 times;
(2) produce the cultivation of Ruan candidiasis bacterial classification: containing glucose 19 g/L, yeast extract paste 9g/L, K2HPO4 1.5 substratum of g/L, MgSO4 1.2g/L, in pH value 4, leavening temperature 30 DEG C, cultivates 8h under rotating speed 180rpm condition;
(3) bacterial classification secondary enlarged culturing: get step (1) gained black liquor and dilute 3 times, the urea adding 3% is nitrogenous source, and the ratio in 1% adds gained bacterial classification in step (2), in pH value 5, leavening temperature 30 DEG C, cultivates 36h under rotating speed 180rpm condition;
(4) black liquor fermentation: add gained strain liquid in step (3) in the ratio of 3% in step (1) gained process black liquor, be 4 in pH value, leavening temperature 35 DEG C, cultivates 36h under rotating speed 180rpm condition;
(5) thalline obtains: filtration step (4) gained fermented liquid, obtains yeast thalline and ferment filtrate;
(6) preparation of fertilizer mother liquor: step (5) gained filtrate is concentrated into dry-matter final concentration 50%, obtains fertilizer mother liquor;
(7) by fertilizer mother liquor and urea, potassium sulfate, potassium primary phosphate, trace element built, namely abundant reaction obtains high-efficiency foliage fertilizer, and weight proportion is as follows: fertilizer mother liquor 65%, urea 5%, potassium sulfate 10%, trace element 5%, potassium primary phosphate 15%.
Trace element in above-described embodiment is the inner complex adopting copper iron, manganese, zinc, boron, the Sino-German several element of molybdenum.
In addition, the present invention is not limited to above-mentioned embodiment, based on the technique effect that same approach reaches, all belongs to protection scope of the present invention.
Claims (5)
1. utilize black liquid to produce a method for yeast and fertilizer mother liquor, it is characterized in that: mainly comprise following processing step:
(1) black liquid pre-treatment: black liquor is crossed and filtered insoluble substance, gas explosion process 30min ~ 120min, adds diluted acid or diluted alkaline regulates black liquor pH to 4 ~ 7, redilution 0.1 ~ 5 times;
(2) cultivation of Ruan candidiasis bacterial classification is produced: containing glucose 19g/L, yeast extract paste 9g/L, K2HPO4 1.5 substratum of g/L and MgSO4 1.2g/L, pH value 4 ~ 7, leavening temperature 20 DEG C ~ 35 DEG C, cultivates 8h ~ 36h under rotating speed 180rpm condition;
?(3) bacterial classification secondary enlarged culturing: get step (1) gained black liquor and again dilute 0.1 ~ 10 times, the urea adding 0.01% ~ 5% is nitrogenous source, ratio in 0.1% ~ 10% adds gained bacterial classification in step (2), in pH value 4 ~ 7, leavening temperature 20 DEG C ~ 35 DEG C, cultivates 8h ~ 36h under rotating speed 180rpm condition;
(4) black liquor fermentation: add gained strain liquid in step (3) in the ratio of 0.1% ~ 10% in step (1) gained process black liquor, in pH value 4 ~ 7, leavening temperature 20 DEG C ~ 35 DEG C, cultivates 8h ~ 36h under rotating speed 180rpm condition;
(5) thalline obtains: carried out filtering or centrifugal treating by step (4) gained fermented liquid, obtains yeast thalline and ferment filtrate;
(6) fertilizer mother liquor preparation: step (5) gained ferment filtrate is concentrated into dry-matter final concentration 30% ~ 50%, obtains fertilizer mother liquor.
2. a kind of method utilizing black liquid to produce yeast and fertilizer mother liquor according to claim 1, it is characterized in that: the diluted acid in described step (1) is hydrochloric acid or sulfuric acid or phosphoric acid, diluted alkaline is sodium hydroxide or potassium hydroxide or sodium carbonate or salt of wormwood.
3. a kind of method utilizing black liquid to produce yeast and fertilizer mother liquor according to claim 1, it is characterized in that: utilize described fertilizer mother liquor to prepare the method for fertilizer, with described fertilizer mother liquor be base fluid and urea, at least one in potassium sulfate, potassium primary phosphate, trace element is composite, make fertilizer, each composition weight proportioning is: fertilizer mother liquor 10% ~ 80%, urea 5% ~ 30%, potassium sulfate 5% ~ 20%, trace element 2% ~ 20%, potassium primary phosphate 5% ~ 20%, trace element is selected from copper, iron, manganese, zinc, boron, molybdenum.
4. a kind of method utilizing black liquid to produce yeast and fertilizer mother liquor according to claim 1, it is characterized in that: utilize described fertilizer mother liquor to prepare the method for fertilizer, by described fertilizer mother liquor and urea, potassium sulfate, potassium primary phosphate, trace element built, namely abundant reaction obtains high-efficiency foliage fertilizer, weight proportion is: fertilizer mother liquor 10% ~ 70%, urea 5% ~ 25%, potassium sulfate 5% ~ 18%, trace element 2% ~ 10%, potassium primary phosphate 5% ~ 15%.
5. a kind of method utilizing black liquid to produce yeast and fertilizer mother liquor according to claim 1, it is characterized in that: utilize described fertilizer mother liquor to prepare the method for fertilizer, by described fertilizer mother liquor and urea, potassium sulfate, potassium primary phosphate, trace element built, abundant mixing, granulation, drying, namely sieve obtains black liquid fertilizer, its weight proportion is: fertilizer mother liquor 10% ~ 70%, urea 5% ~ 25%, potassium sulfate 5% ~ 18%, trace element 2% ~ 10%, potassium primary phosphate 5% ~ 15%.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104892074A (en) * | 2015-06-06 | 2015-09-09 | 成都市新都化工股份有限公司 | Fertilizer specially used for ornamental flower and preparation method of fertilizer |
| CN105541436A (en) * | 2015-12-31 | 2016-05-04 | 南宁太美生物科技有限公司 | Multi-nutrient liquid compound fertilizer |
| CN106906261A (en) * | 2017-05-10 | 2017-06-30 | 山东农业大学 | A kind of method that utilization biofermentation extracts biochemical fulvic acid from black liquid |
| CN107056359A (en) * | 2016-04-08 | 2017-08-18 | 上海万力华生物科技有限公司 | A kind of Water soluble fertilizer and its preparation method and application |
| CN108179650A (en) * | 2017-12-05 | 2018-06-19 | 白博 | A kind of non-wood fiber wheat straw production technology using black liquor of pulp making production Yellow humic acid fertilizer |
| CN111771454A (en) * | 2020-08-24 | 2020-10-16 | 山东农业大学 | Rice salt-tolerant seed coating component prepared based on papermaking black liquor and application method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104892074A (en) * | 2015-06-06 | 2015-09-09 | 成都市新都化工股份有限公司 | Fertilizer specially used for ornamental flower and preparation method of fertilizer |
| CN105541436A (en) * | 2015-12-31 | 2016-05-04 | 南宁太美生物科技有限公司 | Multi-nutrient liquid compound fertilizer |
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| CN108179650A (en) * | 2017-12-05 | 2018-06-19 | 白博 | A kind of non-wood fiber wheat straw production technology using black liquor of pulp making production Yellow humic acid fertilizer |
| CN108179650B (en) * | 2017-12-05 | 2021-03-02 | 白博 | Non-wood fiber biochemical mechanical pulp production process for producing fulvic acid fertilizer by using pulping black liquor |
| CN111771454A (en) * | 2020-08-24 | 2020-10-16 | 山东农业大学 | Rice salt-tolerant seed coating component prepared based on papermaking black liquor and application method thereof |
| CN111771454B (en) * | 2020-08-24 | 2023-09-12 | 山东农业大学 | Rice salt-resistant seed coating component prepared based on papermaking black liquor and application method thereof |
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Application publication date: 20141224 |