CN108056149B - Fresh jellyfish cryopreservation method - Google Patents

Fresh jellyfish cryopreservation method Download PDF

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CN108056149B
CN108056149B CN201711365860.5A CN201711365860A CN108056149B CN 108056149 B CN108056149 B CN 108056149B CN 201711365860 A CN201711365860 A CN 201711365860A CN 108056149 B CN108056149 B CN 108056149B
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jellyfish
strips
fresh
freezing
shreds
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CN108056149A (en
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刘淇
赵玲
曹荣
孙慧慧
温锦力
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Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B4/00General methods for preserving meat, sausages, fish or fish products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B4/00General methods for preserving meat, sausages, fish or fish products
    • A23B4/03Drying; Subsequent reconstitution
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B4/00General methods for preserving meat, sausages, fish or fish products
    • A23B4/06Freezing; Subsequent thawing; Cooling
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B4/00General methods for preserving meat, sausages, fish or fish products
    • A23B4/06Freezing; Subsequent thawing; Cooling
    • A23B4/08Freezing; Subsequent thawing; Cooling with addition of chemicals or treatment with chemicals before or during cooling, e.g. in the form of an ice coating or frozen block
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B4/00General methods for preserving meat, sausages, fish or fish products
    • A23B4/10Coating with a protective layer; Compositions or apparatus therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/3562Sugars; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/358Inorganic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Microbiology (AREA)
  • Inorganic Chemistry (AREA)
  • Meat, Egg Or Seafood Products (AREA)

Abstract

The invention aims to provide a method for freezing and preserving fresh jellyfish, which comprises the steps of soaking fresh jellyfish in a K-type carageenan solution and a potassium chloride solution, and then freezing and preserving, thereby effectively overcoming the problem of low water retention rate of the existing jellyfish freezing processing, and improving the water retention rate of the frozen jellyfish from below 50% to above 80%. And the fresh jellyfish preserved by the method of the invention has good taste.

Description

Fresh jellyfish cryopreservation method
Technical Field
The invention belongs to the technical field of aquatic product storage and processing, and particularly relates to a fresh jellyfish cryopreservation method.
Background
Jellyfish, also known as jellyfish, belongs to the phylum coelenterate, the class of potted jellyfish, the order of the Megalobatrachales, the family of Megalobatrachales, the genus jellyfish. Jellyfishes are widely distributed on coastal areas of China, and are main large-scale economic jellyfishes in coastal areas of China. The product has rich protein, inorganic salts, vitamins, etc., and high nutritive value, and is a nutritious food with high protein, low fat, and low calorie. The jellyfish also has the medicinal effects of clearing heat, reducing phlegm, preventing and treating tumors and the like, and has high economic value.
The water content of the jellyfish is more than 95 percent, and the jellyfish is harvested in summer and autumn at higher temperature, so that the jellyfish is easy to rot and deteriorate after being treated in time, and the quality is poor and the yield is low due to the improper processing method. The traditional method for processing fresh jellyfish is that after the fresh jellyfish is captured, the head and the body are separated, the internal organs and blood coats are removed, then the head and the body are respectively salted by salt and alum according to a specific proportion, the juice is filtered out to obtain salted jellyfish, and the jellyfish processed by three times of salt and alum (or mixture of primary alum and secondary salt and alum) is commonly called three alum extraction drying jellyfish. However, the jellyfish processed by the method has the problem of high aluminum ion content, and the aluminum ions have obvious toxicity to human bodies, so that the health of the human bodies can be harmed by eating the jellyfish product with high aluminum content frequently.
In previous studies, applicants have attempted to directly freeze fresh jellyfish; however, if the jellyfish is frozen and stored according to the conventional method, the result shows that the frozen jellyfish is seriously dehydrated after being thawed, the weight loss rate after being thawed exceeds 50 percent, the mouthfeel is not good, and the crispness is lost; the applicant has also tried to freeze after treatment with a water-retaining agent such as a complex phosphate, but no significant effect is produced. The reason is that the water content of the fresh jellyfish is more than 95 percent, and ice crystals are more easily generated in the freezing process.
Disclosure of Invention
The invention aims to provide a method for freezing and preserving fresh jellyfishes, thereby making up for the defects of the prior art.
The invention relates to a method for freezing and preserving fresh jellyfish, which comprises the following steps:
1) pretreatment of fresh jellyfish
The pretreatment comprises the steps of separating the head part and the umbrella part of the caught fresh jellyfish along the belly of the umbrella body by a knife, removing the jellyfish in the body cavity, and separately processing the head part and the umbrella part of the jellyfish; removing white cream film and blood coat from the umbrella part of the jellyfish, and washing with seawater;
2) shredding
Cutting the umbrella part of the jellyfish treated in the step 1) into thick threads with the thickness of 5 mm-10 mm and the width of 5 mm-10 mm by a shredder;
3) dehydration treatment
Mechanically stirring and dehydrating the cut jellyfish shreds, wherein the stirring speed is 10-60 r/min, and the stirring time is 3-5 h;
4) soaking treatment
Firstly, immersing jellyfish shreds in carrageenin solution (the material-liquid ratio is 0.5-1: 1) with the mass-volume ratio g/ml of 0.1-0.3% for 2-3 h, then fishing out the jellyfish shreds, immersing the jellyfish shreds in potassium chloride solution with the mass-volume ratio g/ml of 0.1-0.2% for 1-2 h (the material-liquid ratio is 1:1), fishing out the jellyfish shreds, washing the jellyfish shreds with water, and draining;
wherein the carrageenan is K-type carrageenan sodium salt, and the molecular weight is 20000-50000D;
wherein the temperature of the carragheenan solution is 5-20 ℃, and the temperature of the potassium chloride solution is 0-10 ℃;
5) quick freezing
Placing the soaked jellyfish strips at-30 ℃ for quick freezing for 30-60 min;
6) storage of
Packaging the quick-frozen jellyfish strips, and then transferring the packaged jellyfish strips into a refrigeration house at the temperature of-18 ℃ or below for long-term storage; preferably, the temperature fluctuation during storage should not exceed 2 ℃.
The invention uses K-type carageenan solution and potassium chloride solution to soak fresh jellyfish, and then carries out freezing preservation, thereby effectively overcoming the problem of low water retention rate of the existing jellyfish freezing processing, and improving the water retention rate of the frozen jellyfish from less than 50 percent to more than 80 percent. And the fresh jellyfish preserved by the method of the invention has good taste.
Detailed Description
The method aims to solve the problem that the salt content and the aluminum ion content of the prepared jellyfish exceed the standard due to the fact that a large amount of salt and alum are used in the existing three-alum processing method or the rapid pickling method of the jellyfish; the applicant engaged in research on jellyfish processing for many years; however, the existing seafood freezing method is adopted for freezing and storing, and obvious dehydration phenomenon can occur after thawing. The treatment with a water-retaining agent such as a composite phosphate and the like followed by freezing was attempted, but no significant effect was produced. The reason is that the water content of the fresh jellyfish is more than 95 percent, and ice crystals are more easily generated in the freezing process. Therefore, the applicant finally obtains the method of the invention by screening the oligosaccharide species and the concentration of the soaking solution by using marine polysaccharides and oligosaccharides.
The present invention will be described in detail with reference to examples.
Example 1: basic steps and screening procedures of the method of the invention
The basic steps of the method of the invention are as follows:
1) shredding the pretreated jellyfish:
the method for pretreating fresh jellyfish comprises the following steps: separating the head part and the umbrella part of the caught fresh jellyfish along the belly of the umbrella body by a knife, removing the jellyfish in the body cavity, and separately processing the head part and the umbrella part of the jellyfish; removing white cream film and blood coat from the umbrella part of the jellyfish, and washing with seawater;
shredding the pretreated jellyfish, and cutting the jellyfish into thick shreds with the thickness of 5-10 mm and the width of 5-10 mm by a shredder;
2) dehydration treatment
Mechanically stirring and dehydrating the cut jellyfish shreds, wherein the stirring speed is 10-60 r/min, and the stirring time is 3-5 h;
3) soaking treatment
Firstly, immersing jellyfish shreds in 0.1-0.3% carragheenan solution (the material-liquid ratio is 0.5-1: 1) for 2-3 h; then fishing out and soaking the mixture for 1 to 2 hours (the material-liquid ratio is 1:1) by using 0.1 to 0.2 percent potassium chloride solution, fishing out and cleaning the mixture by using water, and draining;
wherein the carrageenan is K-type carrageenan sodium salt, and the molecular weight is 20000-50000D. The preparation method comprises the following steps of decomposing K-type carrageenan serving as a raw material by using carrageenase, carrying out molecular weight classification by using an ultrafiltration membrane to obtain the K-type carrageenan with the molecular weight of 20000-50000D, adding sodium hydroxide to convert the K-type carrageenan into sodium salt, and carrying out nanofiltration desalination, concentration and drying to obtain the carrageenan.
Wherein the temperature of the carrageenan solution is 5-20 ℃, and the temperature of the potassium chloride solution is 0-10 DEG C
5) Quick freezing
Placing the soaked jellyfish at-30 deg.C, and quick freezing for 30 min;
6) storage of
Packaging the quick-frozen jellyfish strips, and then transferring the packaged jellyfish strips into a refrigeration house at the temperature of-18 ℃ or below for storage; preferably, the temperature fluctuation during storage should not exceed 2 ℃.
In the process of establishing the invention, different types and different molecular weights of oligosaccharides are screened, so that the water retention rate of the frozen jellyfish has the best effect, and the types and the molecular weights of the oligosaccharides used by the invention are finally determined.
Table 1: influence of K-type carrageenan sodium salts with different molecular weights on water retention rate of jellyfish
Figure BDA0001512881220000041
The detection method of the water retention rate comprises the following steps:
A. the unfreezing method comprises the following steps:
removing package of frozen shredded jellyfish, and weighing (m)0To an accuracy of 0.1g) in a thawing container, carefully agitated under normal flow water at a temperature below 20 ℃ so that the sample is not damaged, thawed until the surface ice layer is melted and all visible or accessible ice coating is removed and no hard heart is felt by hand. Placing completely thawed jellyfish in a basket with meshes, draining for 5min at an inclined angle, and weighing (m)1To the nearest 0.1 g).
B. Formula for calculating water retention
Water retention (%) ═ m1/m0x 100。
Example 2
Freezing and storing fresh jellyfish harvested from yellow sea in 2016, 8 months and 11 days, and specifically comprises the following steps:
1) shredding the pretreated jellyfish:
the method for pretreating fresh jellyfish comprises the following steps: separating the head part and the umbrella part of the caught fresh jellyfish along the belly of the umbrella body by a knife, removing the jellyfish in the body cavity, and processing the jellyfish head and the jellyfish skin separately. Wherein the white cream film and blood coat of the umbrella part of the jellyfish are removed, and the jellyfish is washed clean by seawater;
shredding the pretreated jellyfish, and cutting into 5mm thick and 8mm wide thick shreds by a shredder;
2) dehydration treatment
Mechanically stirring and dehydrating 20Kg of jellyfish shreds, wherein the stirring speed is 20 r/min, and the stirring time is 3 h;
3) soaking treatment
Firstly, immersing jellyfish shreds in 10L carrageenin solution (the material-liquid ratio is 0.5:1) with the temperature of 18 ℃ and the concentration of 0.2% for 3 hours; then fishing out and soaking in 0.2% potassium chloride solution at 4 ℃ for 2h, fishing out and washing with water, and draining;
the preparation method of the carrageenan solution comprises the following steps:
dissolving K-type carrageenan sodium salt with the molecular weight of 20000-50000D in an aqueous solution, and stirring to prepare a carrageenan solution;
5) quick freezing
Placing the soaked jellyfish at-30 deg.C, and quickly freezing for 35 min;
6) storage of
Packaging the quick-frozen jellyfish strips, and storing in a refrigeration house at-18 ℃.
The frozen jellyfish strips stored for 3 months and 6 months were taken out for quality inspection at 2016, 11 months and 2017, 2, 11 months, respectively.
The result shows that the water retention rates of the thawed jellyfish shreds preserved by the technology are 91 percent and 87 percent respectively; the result proves that the jellyfish can be effectively preserved; and after the preserved jellyfish is processed, the taste is not obviously different from that of fresh jellyfish.
Table 2: water retention after thawing of jellyfish results of example 2
Freezing storage time Frozen fresh jellyfish The invention relates to frozen fresh shredded jellyfish
3 months old 46% 91%
6 months old 39% 87%
Meanwhile, the aluminum content of the thawed jellyfish is measured by an inductively coupled plasma mass spectrometry method (GB/T23374-2009) for measuring aluminum in food according to the national standard, and the result shows that the aluminum ion content of the jellyfish stored by the method is obviously lower than that of other existing jellyfish storage methods (Table 3).
Table 3: data of aluminum content in jellyfish wire of example 2
Figure BDA0001512881220000061

Claims (4)

1. A method for freezing and preserving fresh jellyfish is characterized by comprising the following steps:
1) pretreatment of fresh jellyfish
Separating the head part and the umbrella part of the caught fresh jellyfish along the belly of the umbrella body by a knife, removing the jellyfish in the body cavity, and separately processing the head part and the umbrella part of the jellyfish; removing white cream film and blood coat from the umbrella part of the jellyfish, and washing with seawater;
2) shredding
Cutting the jellyfish umbrella part processed in the step 1) into jellyfish shreds by a shredder;
3) dehydration treatment
Mechanically stirring and dehydrating the cut jellyfish shreds, wherein the stirring speed is 10-60 r/min, and the stirring time is 3-5 h;
4) soaking treatment
Firstly soaking jellyfish strips in carragheen solution for 2-3 h, then fishing out the jellyfish strips, soaking the jellyfish strips in 0.1-0.2% potassium chloride solution for 1-2 h, fishing out the jellyfish strips, cleaning the jellyfish strips with water, and draining;
the carrageenan is K-type carrageenan sodium salt with the molecular weight of 20000-50000D, and the concentration is 0.1% -0.3%;
5) quick freezing
Placing the soaked jellyfish strips at-30 ℃ for quick freezing for 30-60 min;
6) storage of
Packaging the quick-frozen jellyfish strips, and transferring the packaged jellyfish strips into a refrigeration house at the temperature of-18 ℃ or below for long-term storage.
2. The method of claim 1, wherein the jellyfish shreds in step 2) are 5mm to 10mm thick and 5mm to 10mm wide.
3. The method according to claim 1, wherein the temperature of the carrageenan solution in step 4) is 5 to 20 ℃.
4. The method as claimed in claim 1, wherein the temperature of the potassium chloride solution in the step 4) is 0-10 ℃.
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CN112535199A (en) * 2020-12-07 2021-03-23 中国水产科学研究院黄海水产研究所 Rapid dehydration method for fresh jellyfishes

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JP5276860B2 (en) * 2008-03-13 2013-08-28 株式会社日立ハイテクノロジーズ Scanning electron microscope
CN101822284A (en) * 2010-04-26 2010-09-08 江苏大学 Method for improving frozen fillet structure by utilizing phosphorus-free quality improver
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