CN108048906A - A kind of DNA is oriented to gold nano-crystal and preparation method and application - Google Patents
A kind of DNA is oriented to gold nano-crystal and preparation method and application Download PDFInfo
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- 239000010931 gold Substances 0.000 title claims abstract description 106
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 97
- 239000002159 nanocrystal Substances 0.000 title claims abstract description 84
- 238000002360 preparation method Methods 0.000 title claims abstract description 37
- 239000003638 chemical reducing agent Substances 0.000 claims abstract description 25
- SJUCACGNNJFHLB-UHFFFAOYSA-N O=C1N[ClH](=O)NC2=C1NC(=O)N2 Chemical compound O=C1N[ClH](=O)NC2=C1NC(=O)N2 SJUCACGNNJFHLB-UHFFFAOYSA-N 0.000 claims abstract description 19
- 238000010521 absorption reaction Methods 0.000 claims abstract description 18
- 238000006243 chemical reaction Methods 0.000 claims abstract description 17
- 201000011510 cancer Diseases 0.000 claims abstract description 12
- 238000007626 photothermal therapy Methods 0.000 claims abstract description 7
- 238000011065 in-situ storage Methods 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 76
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000007864 aqueous solution Substances 0.000 claims description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 4
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- 239000001509 sodium citrate Substances 0.000 claims description 2
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 2
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- C—CHEMISTRY; METALLURGY
- C30—CRYSTAL GROWTH
- C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
- C30B29/00—Single crystals or homogeneous polycrystalline material with defined structure characterised by the material or by their shape
- C30B29/02—Elements
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B22—CASTING; POWDER METALLURGY
- B22F—WORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
- B22F9/00—Making metallic powder or suspensions thereof
- B22F9/16—Making metallic powder or suspensions thereof using chemical processes
- B22F9/18—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds
- B22F9/24—Making metallic powder or suspensions thereof using chemical processes with reduction of metal compounds starting from liquid metal compounds, e.g. solutions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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- C—CHEMISTRY; METALLURGY
- C30—CRYSTAL GROWTH
- C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
- C30B7/00—Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions
- C30B7/14—Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions the crystallising materials being formed by chemical reactions in the solution
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Abstract
The present invention relates to the preparation method that a kind of DNA is oriented to gold nano-crystal, which comprises the following steps：DNA molecular is modified in gold nanorods surface first, gold nanorods/DNA compounds are made, then under the guiding role of gold nanorods/DNA compounds, makes tetra chlorauric acid and reducing agent that in-situ reducing reaction generation gold nano-crystal occur in solution system.The invention further relates to DNA made from the preparation method to be oriented to gold nano-crystal, in 780nm visible light regions to the second infrared region of 1100nm, the invention further relates to the DNA to be oriented to application of the gold nano-crystal in malignant tumour photo-thermal therapy for the position of its surface plasma body resonant vibration maximum absorption band.Compared with the prior art, preparation method that DNA of the present invention is oriented to gold nano-crystal has the advantages that be easily achieved, manufacturing cost is low, the pattern and performance of gold nano-crystal can be regulated and controled.
Description
Technical field
The present invention relates to technical field of nanometer material preparation, and gold nano-crystal and its system are oriented to more particularly to a kind of DNA
Preparation Method and application.
Background technology
In recent years, it is special same so as to generate pattern using the assembling of DNA (DNA) chain regulation and control gold nanoparticle
When the nano material with excellent performance become technical field of nanometer material preparation an important directions.Gold nanoparticle is because of it
The performances such as itself excellent light, electricity, catalysis and biocompatibility, are widely used in the necks such as spectrum, biomedicine, environment and catalysis
Domain.Gold nanoparticle performance quality it is associated with pattern, surface roughness and the size of particle etc., so study shape with
The preparation of the controllable gold nanoparticle of size is always a big focus of work of researchers.
The study found that being acted on using the stent of DNA chain, the gold nanoparticle for being modified with DNA can be by in-situ reducing
Mode prepares the gold nano-material with specific morphology structure and performance.And because DNA be in itself it is a kind of have have structure more
The molecule of form, and various configuration DNA also has different rigidity, so can regulate and control generate using DNA as guide molecule
Nano particle pattern, and then influence its performance.In the environment of cancer discoloration is talked now, photo-thermal therapy is compared to other cancers
Therapeutic modality, such as surgery excision, Radiotherapy, chemotherapy etc., have it is easily operated, Small side effects and treatment cycle compared with
The advantages such as short, thus receive significant attention.Nano material with plasmon characteristic, such as gold nanorods, because that can incite somebody to action
The light of near infrared region changes into heat, can be applied to as light thermit powder in the research of photo-thermal therapy cancer, become researchers
The first choice that Application of micron is treated in cancer diagnosis.
In order to have better cancer diagnosis and a therapeutic effect, researchers attempt by the compound mode of multiple modification come
The photothermal conversion efficiency of reinforcing material, this undoubtedly adds synthesis difficulty and improves manufacturing cost.
The preparation method to form the special nano particle of pattern is oriented on primary structure DNA, that is, single stranded DNA to have much
Document report, but other a variety of different structure DNA are oriented to the preparation side to form pattern and the regulatable gold nano-crystal of performance
The relevant report of method is not much at present sees.
The content of the invention
Based on this, it is a first object of the present invention to provide a kind of DNA is oriented to the preparation method of gold nano-crystal, tool
Have be easily achieved, manufacturing cost is low, the advantages of can regulating and controlling to the pattern and performance of gold nano-crystal.
The technical solution that the present invention takes is as follows：
A kind of DNA is oriented to the preparation method of gold nano-crystal, comprises the following steps：DNA molecular is modified in Jenner first
Rice stick surface, is made gold nanorods/DNA compounds, then under the guiding role of gold nanorods/DNA compounds, makes tetrachloro golden
In-situ reducing reaction generation gold nano-crystal occurs in solution system for acid and reducing agent.
Preparation method of the present invention modifies DNA molecular in gold nanorods surface, using the guiding role of DNA chain,
The gold nano-crystal with specific morphology structure and performance, energy are prepared by way of in-situ reducing on the basis of gold nanorods
The pattern of Effective Regulation gold nano-crystal, and then purposefully control its performance characteristics.Moreover, compared to the compound system of multiple modification
The method of standby gold nano-material, the preparation method have easy to operate and realize, simplify preparation flow, reduce manufacturing cost
Advantage may advantageously facilitate the technology development of photo-thermal therapy cancer.
Further, the DNA molecular is the single-stranded type DNA molecular of primary structure, secondary structure double chain DNA molecular, hair
Any one or more in clip-type DNA molecular and tertiary structure Y type DNA moleculars, and the DNA molecular is modified with mercapto for one end
The DNA molecular of base (- SH).
The DNA molecular of various configuration can be used as guide molecule, various configuration DNA in preparation method of the present invention
With different rigidity, the Effective Regulation to nano particle pattern is realized, obtain the gold nano-crystal for possessing different performance characteristics.
Further, the draw ratio of the gold nanorods is 1~10, and preferred value is 1~6.
Further, it is 1 according to the molar ratio of golden (Au) and DNA molecular:200~2000, preferred value 1:400~
1200, DNA molecular is modified, gold nanorods/DNA compounds are made in gold nanorods surface.
Further, the reducing agent is any one in sodium borohydride, ascorbic acid, hydroxylamine hydrochloride and sodium citrate
It is or a variety of.
Further, the molar ratio of the reducing agent and tetra chlorauric acid is 1~20.
Further, the pH value of reaction solution be 1~8, preferred value be 3~6, the molar concentration of wherein reducing agent be less than or
Equal to 1mol/L, preferred value is 0~0.1mol/L, not including 0.If the pH value of reaction solution is excessive or too small can influence Jenner
The pattern of meter Jing Ti or even guiding growth cannot occur.
Further, the preparation method specifically includes following steps：
(1) prepare and be oriented to solution：Molar ratio according to gold and DNA molecular is 1:200~1000, take the Jenner of phase application amount
Rice stick aqueous solution and DNA solution mixing, it is 0.01~0.5mol/L, preferably molar concentration to add sodium chloride to its molar concentration
For 0.1~0.5mol/L, then 18~48h of aging, is made gold nanorods/DNA complex solutions, then centrifugation point is carried out to it
From, then separating obtained gold nanorods/DNA complex precipitates are resuspended in 1.5mL pure water, acquired solution as be oriented to it is molten
Liquid is spare；
(2) growth-promoting media is prepared：The tetra chlorauric acid aqueous solution of 100~1000 μ L mass concentrations 2% is taken to be added to 10~80mL
In water, after mixing, the sodium hydroxide solution for adding in molar concentration 0.01~0.2mol/L adjusts pH value to 3~9, and acquired solution is
Growth-promoting media；
(3) reducing agent solution is prepared：0.01~0.2g reducing agents is taken to be added in 10~100mL water, after mixing, addition rubs
The sodium hydroxide solution that your concentration is 0.01~0.2mol/L adjusts pH value to 2~8, and acquired solution is reducing agent solution；
(4) gold nano-crystal is prepared：20~140 μ L growth-promoting medias are added to 100~200 μ L to be oriented in solution, concussion is mixed
It is 8~18 according to the molar ratio of reducing agent and tetra chlorauric acid after even, the reducing agent solution of phase application amount is added in, after shaking mixing
React 10~30min；
(5) separating-purifying：After the completion of reaction, reaction solution is centrifuged, then by separating obtained gold nanocrystals
Body product, which is resuspended in pure water, to be preserved.
The second object of the present invention is to provide DNA made from preparation method described in any of the above-described and is oriented to gold nano-crystal,
The position of the surface plasma body resonant vibration maximum absorption band of the gold nano-crystal is in 780nm visible light regions to 1100nm second
In infrared region.
Gold nano-crystal prepared by the method for the present invention can obtain different-shape by changing synthesis condition, and to its table
The position of surface plasma resonance maximum absorption band from 780nm visible light regions to the second infrared region of 1100nm in adjusted
Control.
The third object of the present invention is to provide above-mentioned DNA and is oriented to gold nano-crystal answering in malignant tumour photo-thermal therapy
With.
Surface plasma body resonant vibration maximum absorption band can be inhaled in the gold nano-crystal of near infrared region (780nm~1100nm)
Medically common 808nm near-infrared lasers are received, then convert light into thermal energy rapidly, so as to cause on gold nano-crystal surface
It is rapidly heated.Above-mentioned gold nano-crystal have in room temperature and do not receive under illumination condition or it is harmless to cell and in illumination condition
The lower function of killing people's source cervical cancer cell (Hela cells), therefore it can be used for the photo-thermal therapy of malignant tumour as light thermit powder
In.
The function that the gold nano-crystal kills cancer cell has concentration dependent, and solution concentration is in 200mg/L
Preferable kill cell function can be played when following, preferred concentration range is 50~200mg/L.
In order to better understand and implement, the invention will now be described in detail with reference to the accompanying drawings.
Description of the drawings
Fig. 1 is that the hair clip type DNA of embodiment 1 is oriented to the transmission electron microscope picture for the gold nano-crystal to be formed；
Fig. 2 is ultraviolet-visible-near infrared absorption graph that DNA made from embodiment 1-4 is oriented to gold nano-crystal；
Fig. 3 is oriented to the gold nano-crystal to be formed for hair clip type DNA under the conditions of tetra chlorauric acid dosages different in embodiment 5
Ultraviolet-visible-near infrared absorption graph；
Fig. 4 is oriented to the gold nano-crystal to be formed for hair clip type DNA under the conditions of tetra chlorauric acid dosages different in embodiment 5
Transmission electron microscope picture；
Fig. 5 is Au different in embodiment 6：Hair clip type DNA is oriented to the gold nano-crystal to be formed under DNA molar ratios
Ultraviolet-visible-near infrared absorption graph；
Fig. 6 is cell survival rate and the relational graph of gold nano-crystal solution concentration in embodiment 7；
Fig. 7 is the external heating curve figure of the gold nanocrystals liquid solution of various concentration in embodiment 8.
Specific embodiment
Reagent and biomaterial in following embodiments unless otherwise instructed, can be obtained from open commercial sources.
Embodiment 1
The present embodiment prepares DNA guiding gold nano-crystals and comprises the following steps：
(1) prepare and be oriented to solution：According to Au：The molar ratio of DNA is 1:600, take the gold nanorods aqueous solution of phase application amount
It is uniformly mixed with DNA solution, the draw ratio of the gold nanorods in the gold nanorods aqueous solution is 3.6, which is hair clip type
The solution of DNA molecular.It is 0.5mol/L to add in sodium chloride to its molar concentration into mixed liquor again, then aging 48h, and gold is made
Nanometer rods/DNA complex solutions, then it is centrifuged, then separating obtained gold nanorods/DNA compounds are sunk
Shallow lake is resuspended in 1.5mL pure water, and acquired solution is as guiding solution for standby.
(2) growth-promoting media is prepared：The tetra chlorauric acid aqueous solution of 100~1000 μ L mass concentrations 2% is taken to be added to 10~80mL
In water, after mixing, the sodium hydroxide solution for adding in 0.01~0.2mol/L of molar concentration adjusts pH value to 3~9, and gained is molten
Liquid is growth-promoting media.
(3) reducing agent solution is prepared：0.01~0.2g reducing agents is taken to be added in 10~100mL water, after mixing, addition rubs
The sodium hydroxide solution that your concentration is 0.01~0.2mol/L adjusts pH value to 2~8, and acquired solution is reducing agent solution.
(4) gold nano-crystal is prepared：40 μ L growth-promoting medias are added to 150 μ L to be oriented in solution, after shaking mixing, according to also
The molar ratio of former agent and tetra chlorauric acid is 8~18, adds in the reducing agent solution of phase application amount, shakes mixing, gained reaction solution
PH value for 3~6, react 15min, observe that reaction solution is gradually transformed into lavender by light brown.
(5) separating-purifying：After the completion of reaction, reaction solution is placed in a centrifuge the rotating speed centrifugation with 6500r/min
15min after gold nano-crystal product to be generated precipitates completely, then is resuspended in pure water and is preserved.
Referring to Fig. 1, it is oriented to the transmission electron microscope picture for the gold nano-crystal to be formed for the hair clip type DNA of the present embodiment.
It may be seen that gold nano-crystal homoepitaxial is on gold nanorods surface.
Embodiment 2
The step of the present embodiment preparation DNA is oriented to gold nano-crystal, operating condition are substantially the same manner as Example 1, difference
Be in：DNA solution used is the solution of the single-stranded type DNA molecular of primary structure in step (1).
Embodiment 3
The step of the present embodiment preparation DNA is oriented to gold nano-crystal, operating condition are substantially the same manner as Example 1, difference
Be in：DNA solution used is the solution of secondary structure double chain DNA molecular in step (1).
Embodiment 4
The step of the present embodiment preparation DNA is oriented to gold nano-crystal, operating condition are substantially the same manner as Example 1, difference
Be in：DNA solution used is the solution of tertiary structure Y type DNA moleculars in step (1).
Referring to Fig. 2, it is oriented to ultraviolet-visible-near infrared absorption of gold nano-crystal for DNA made from embodiment 1-4
Graph.It may be seen that single-stranded type, double chain, hair clip type, Y types DNA are oriented to the surface plasma for the gold nano-crystal to be formed
The position of resonance body maximum absorption band is all in 780nm visible light regions to the second infrared region of 1100nm, but absorption value is respectively not
It is identical, illustrate under identical preparation condition, by selecting the DNA guide molecules of various configuration, it can be achieved that gold nano-crystal light
The regulation and control of absorption characteristic.
Embodiment 5
Under the conditions of different tetra chlorauric acid dosages, gold nano-crystal is prepared with hair clip type DNA guiding, step is as follows：
According to the preparation method of embodiment 1, first implementation steps (1) prepare and are oriented to solution, step (2) prepares growth-promoting media and
Step (3) prepares reducing agent solution, is then respectively used for the growth-promoting media that volume is 20,40,60,80,100,120,140 μ L real
It applies step (4) and prepares gold nano-crystal, other preparation conditions of step (4) are fixed, then obtain seven different reaction solutions, then
To its implementation steps (5) separating-purifying one by one, hair clip type DNA is oriented to be formed under the conditions of obtaining seven different tetra chlorauric acid dosages
Gold nano-crystal sample.
Referring to Fig. 3, it is oriented to the gold to be formed for hair clip type DNA under the conditions of tetra chlorauric acid dosage different in the present embodiment
The ultraviolet-visible of nanocrystal-near infrared absorption graph.It may be seen that it is formed under the conditions of different tetra chlorauric acid dosages
Gold nano-crystal optical absorption characteristics it is significantly different.Referring to Fig. 4, it is tetra chlorauric acid dosage item different in the present embodiment
Hair clip type DNA is oriented to the transmission electron microscope picture for the gold nano-crystal to be formed under part.It may be seen that different tetra chlorauric acid dosage item
The gold nano-crystal pattern formed under part is significantly different.The above results show the dosage by changing reactant tetra chlorauric acid, can
Realize the regulation and control to gold nano-crystal pattern and optical absorption characteristics.
Embodiment 6
In different Au：Under DNA molar ratios, gold nano-crystal is prepared with hair clip type DNA guiding, step is as follows：
According to the preparation method of embodiment 1, respectively according to 1:200,1:400,1:600,1:800,1:1000 Au：DNA
Prepared by molar ratio implementation steps (1) is oriented to solution, other preparation conditions of step (1) are fixed, then obtain five different guiding
Solution, then implementation steps (2) prepare growth-promoting media and step (3) prepares reducing agent solution, then respectively by above-mentioned five guiding
Solution prepares gold nano-crystal for implementation steps (4), other preparation conditions of step (4) are fixed, then obtain five it is different
Reaction solution, then to its implementation steps (5) separating-purifying one by one, obtain five different Au：Hair clip type under DNA molar ratios
DNA is oriented to the gold nano-crystal sample to be formed.
Referring to Fig. 5, it is Au different in the present embodiment：Hair clip type DNA is oriented to the gold to be formed under DNA molar ratios
The ultraviolet-visible of nanocrystal-near infrared absorption graph.It may be seen that different Au：It is formed under DNA molar ratios
The optical absorption characteristics of gold nano-crystal differ, illustrate the molar ratio by changing Au and DNA, it can be achieved that gold nano-crystal
Optical absorption characteristics regulation and control.
Embodiment 7
DNA made from testing example 1 is oriented to the biocompatibility of gold nano-crystal, and step is as follows：
1. the culture of cell
By human hela (Hela) cell culture in DMEM culture mediums, 10% new life is included in DMEM culture mediums
The penicillin of cow's serum NCS, 100units/mL and the streptomysin of 100units/mL.Cell and culture medium are placed in 37 DEG C always
Containing CO2Bio-incubator in carry out cell culture.Hela cells are the cell of adherent type growth, when it covers blake bottle bottom
Will be to cell dissociation when reaching 80~90%, digestive juice used is trypsase.At the Hela cells in blake bottle
It is digested when exponential phase, is then centrifuged for being configured to the cell suspension of 1mL, by haemocytometer to cell into
Row counts, and selects 96 orifice plates, each hole inoculation~104A cell.The orifice plate for being inoculated with Hela cells is placed in 37 DEG C containing 5%CO2
Bio-incubator in, 24 hour cells it is adherent completely after it is for use.
2. test the biocompatibility of gold nano-crystal
By gold nano-crystal made from embodiment 1 and DMEM culture medium solutions be made into respectively 0,10,25,50,75 and
Then the solution of five kinds of various concentrations of 100mg/L respectively takes 100 μ L to be added separately in the hole of the ready orifice plate of step 1, each
Concentration sets 3 multiple holes.Orifice plate is placed in 37 DEG C containing 5%CO again2Bio-incubator in, treat that gold nano-crystal and cell are hatched
After for 24 hours, culture solution in hole is removed, with PBS buffer solutions rinse three times, it is therefore an objective to remove and be not still in cell interaction
Excessive gold nano-crystal in liquid.Then 100 μ L fresh DMEM medium solution are added in per hole, then orifice plate continued to be placed in 37
DEG C contain 5%CO2Bio-incubator in cultivate for 24 hours, 10 μ L CCK-8 solution are added in after taking-up per hole, then place back in incubator
Continue to hatch 1.5h, then orifice plate is placed in microplate reader and is tested and calculated survival rate.Do not add when calculating cell survival rate
Enter cell, the hole for only adding in culture solution as blank well, only add in cell, do not add in the hole of gold nano-crystal as control wells.
Referring to Fig. 6, it is cell survival rate in the present embodiment and the relational graph of gold nano-crystal solution concentration.By the figure
As it can be seen that under different gold nano-crystal solution concentrations, cell survival rate all close to 100%, illustrates gold nanocrystals prepared by the present invention
Body possesses excellent biocompatibility, has broad application prospects in terms of cancer diagnosis with treatment.
Embodiment 8
Gold nano-crystal is oriented to DNA made from embodiment 1 and carries out external heating experiment, step is as follows：
Gold nano-crystal made from embodiment 1 is dispersed in water, is configured to 0,10,25,50,75 and 100mg/L respectively
The solution of five kinds of various concentrations.The gold nanocrystals liquid solution of each concentration takes 1mL to be placed in 1.5mL microcentrifugal tubes, Ran Houyong
Power density is 2W/cm2, wavelength is that the laser of 808nm is irradiated as light source, every to use infrared imaging at regular intervals
Instrument is monitored the temperature for measuring solution, and records data.
Referring to Fig. 7, its external heating curve figure for the gold nanocrystals liquid solution of various concentration in the present embodiment.By this
Figure as it can be seen that in embodiment 1 with hair clip type DNA be oriented to the gold nano-crystal to be formed after laser irradiates within 3min temperature with regard to energy
Rapid rise, and it is notable to heat up, and when solution concentration reaches 100mg/L, temperature can be increased to 52 DEG C within 10 minutes, be higher than
The rise temperature of the raw material gold nanorods of isoconcentration has significant photo-thermal effect, light thermit powder can be used as to be applied to cancer light
Heat cure.
Embodiment described above only expresses the several embodiments of the present invention, and description is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that come for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.
Claims (10)
1. a kind of DNA is oriented to the preparation method of gold nano-crystal, it is characterised in that：Comprise the following steps：DNA molecular is repaiied first
It adorns in gold nanorods surface, gold nanorods/DNA compounds is made, then under the guiding role of gold nanorods/DNA compounds,
Make tetra chlorauric acid and reducing agent that in-situ reducing reaction generation gold nano-crystal occur in solution system.
2. DNA according to claim 1 is oriented to the preparation method of gold nano-crystal, it is characterised in that：The DNA molecular is
The single-stranded type DNA molecular of primary structure, secondary structure double chain DNA molecular, hair clip type DNA molecular and tertiary structure Y type DNA moleculars
In any one or more, and the DNA molecular is modified with the DNA molecular of sulfydryl for one end.
3. DNA according to claim 2 is oriented to the preparation method of gold nano-crystal, it is characterised in that：The gold nanorods
Draw ratio be 1~10.
4. the preparation method of gold nano-crystal is oriented to according to claim 1-3 any one of them DNA, it is characterised in that：According to
The molar ratio of gold and DNA molecular is 1:200~2000, DNA molecular is modified, gold nanorods/DNA is made in gold nanorods surface
Compound.
5. DNA according to claim 4 is oriented to the preparation method of gold nano-crystal, it is characterised in that：The reducing agent is
Any one or more in sodium borohydride, ascorbic acid, hydroxylamine hydrochloride and sodium citrate.
6. DNA according to claim 5 is oriented to the preparation method of gold nano-crystal, it is characterised in that：The reducing agent with
The molar ratio of tetra chlorauric acid is 1~20.
7. DNA according to claim 6 is oriented to the preparation method of gold nano-crystal, it is characterised in that：The pH of reaction solution
It is worth for 1~8, wherein the molar concentration of reducing agent is less than or equal to 1mol/L.
8. DNA according to claim 7 is oriented to the preparation method of gold nano-crystal, it is characterised in that：It specifically includes following
Step：
(1) prepare and be oriented to solution：Molar ratio according to gold and DNA molecular is 1:200~1000, take the gold nanorods of phase application amount
Aqueous solution and DNA solution mixing, it is 0.01~0.5mol/L to add sodium chloride to its molar concentration, then 18~48h of aging,
Gold nanorods/DNA complex solutions are made, then it is centrifuged, it is then that separating obtained gold nanorods/DNA is multiple
It closes object precipitation to be resuspended in 1.5mL pure water, acquired solution is as guiding solution for standby；
(2) growth-promoting media is prepared：The tetra chlorauric acid aqueous solution of 100~1000 μ L mass concentrations 2% is taken to be added in 10~80mL water,
After mixing, the sodium hydroxide solution for adding in 0.01~0.2mol/L of molar concentration adjusts pH value to 3~9, and acquired solution is growth
Liquid；
(3) reducing agent solution is prepared：0.01~0.2g reducing agents is taken to be added in 10~100mL water, after mixing, addition mole is dense
The sodium hydroxide solution spent for 0.01~0.2mol/L adjusts pH value to 2~8, and acquired solution is reducing agent solution；
(4) gold nano-crystal is prepared：20~140 μ L growth-promoting medias are added to 100~200 μ L to be oriented in solution, after shaking mixing,
It is 8~18 according to the molar ratio of reducing agent and tetra chlorauric acid, adds in the reducing agent solution of phase application amount, react 10 after shaking mixing
~30min；
(5) separating-purifying：After the completion of reaction, reaction solution is centrifuged, then separating obtained gold nano-crystal is produced
Object is resuspended in pure water and preserves.
9. DNA made from claim 1-8 any one of them preparation methods is oriented to gold nano-crystal, it is characterised in that：Surface
The position of plasma resonance maximum absorption band is in 780nm visible light regions to the second infrared region of 1100nm.
10. the DNA described in claim 9 is oriented to application of the gold nano-crystal in malignant tumour photo-thermal therapy.
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Application publication date: 20180518 |