CN107996285A - A kind of method of Huashan pine seed seedling-raising inoculation India truffle - Google Patents
A kind of method of Huashan pine seed seedling-raising inoculation India truffle Download PDFInfo
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- CN107996285A CN107996285A CN201711228107.1A CN201711228107A CN107996285A CN 107996285 A CN107996285 A CN 107996285A CN 201711228107 A CN201711228107 A CN 201711228107A CN 107996285 A CN107996285 A CN 107996285A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The present invention discloses a kind of method of Huashan pine seed seedling-raising inoculation India truffle, belongs to wild-type strain planting technology field.First carry out disinfection Huashan pine seed pretreatment, select fresh India truffle to be equipped with sterile water after crushing and obtain ferfas strain liquid, by virgin soil soil 20 40%, river sand 5 15%, perlite 5 15%, the weight ratio feeding for burning soil 15 25%, mycorhiza soil 30 40%, it is uniformly mixed after crushing and adjusts appropriate moisture content, obtains inoculation matrix fine powder；Then the seedbed for being filled with inoculation matrix is set in greenhouse, and pretreated Huashan pine seed is immersed in ferfas strain liquid after 24 36h and is sown into seedbed, seedling is taken out after growing to about 10 12cm, it is immersed in again in ferfas strain liquid after its disconnected main root and is inoculated with 3 5min, packed after taking-up in greenhouse into transplantation of seedlings, seedling management is until seedling grows up to mycorhiza sapling.There is method is reliable, rate of vaccination is high, cost is low etc..
Description
Technical field
The present invention relates to a kind of India truffle inoculation method, especially a kind of Huashan pine seed seedling-raising inoculation India truffle
Method, belongs to wild-type strain planting technology field.
Background technology
India truffle (Tuber indicum), also known as truffle, popular name pig encircle bacterium, without ma fruit etc., are referred to as " the world first
Name bacterium ".India truffle is rich in 17 kinds of amino acid, 8 kinds of vitamins, suitable protein and androsterone, sterol, sphingolipid, fat
Acid, amino acid and trace element etc. more than 50 plants physiologically active ingredient, and cannot be synthesized containing human body itself 8 kinds of amino acid, zinc,
The essential nutrients such as manganese, iron, calcium, phosphorus, selenium, with medicinal valencys such as strengthen immunity, anti-aging, beneficial stomach, clear god, hemostasis, treatment hemorrhoid
Value, has active anticancer, there is certain inhibitory action to cancer cell, can excite brain cell activity.Due to wild India truffle
Low output by artificial cultivation, it is necessary to meet ever-increasing market demand, and effective inoculation method is not only India's block
The premise of bacterium Mycorrhizal production, and the key of ferfas cultivation success or failure.
Huashan pine（Pinus armandii Franch.）It is one of famous aiphyllium kind in Pinaceae, happiness temperature is cool wet
Moisten weather, can not resist cold and damp and hot, slightly resistance to drying is barren.Go out former for building, furniture and wood-fibred raw material of industry material and chemical industry
Material is outer, also higher medicinal health value.Pine branch can control " yellow water " disease, oedema, parasitosis, and rosin can control " dragon " disease, rheumatism
Property arthritis etc., pine-cones can treat cough phlegm asthma, tracheitis, have sore throat, and pine needle can treat rheumatic arthritis, the bruise stasis of blood
Bitterly, influenza, hypertension etc., pine bark can be used for fracture, traumatic hemorrhage, pollen pini external curing carbuncle furuncle carbuncle, long burst
Do not hold back.
At present, though having the cultivation inoculation technique on India truffle on a small quantity both at home and abroad, and apply to a certain extent manually
In cultivation practices, but rest on the exploratory stage, technology is also remote immature more.Huashan pine as India truffle host plant it
One, attract attention in the artificial cultivation of India truffle, but existing Huashan pine inoculation technique is still immature, and effect of inoculation is paid no attention to
Think, success ratio of inoculation is relatively low, causes the cost increase of India truffle cultivation, yield to be limited, it is difficult to promote and apply, it is necessary to study
The Huashan pine India truffle Inoculation Method of effect.
The content of the invention
The technical problem to be solved in the present invention is low, of high cost for success ratio of inoculation in existing India truffle artificial cultivation
The deficiencies of, propose a kind of method of Huashan pine seed seedling-raising inoculation India truffle, by host plant seedling culture and build bacterium
Kind growing environment, improves India truffle success ratio of inoculation, reduces production cost.
In order to solve the above technical problems, the present invention provides a kind of method of Huashan pine seed seedling-raising inoculation India truffle, its
Comprise the following steps that：
（1）Seeds preprocess：High-quality Huashan pine seed is taken, it is clean with sterile water wash, it is then placed in the more of concentration 0.05-0.1%
15-30min is soaked in bacterium spirit to carry out disinfection sterilization treatment, with aseptic water washing 2-3 times after taking-up, is placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, is first cleaned up with tap water, is then rushed with sterile water
Wash 2-3 times and be placed on the smalls shape that granularity 50-80 mesh is crushed in pulverizer, then by 1：The volume ratio of 5-10 adds sterile water,
It is spare to obtain India truffle strain liquid；
（3）Prepare inoculation matrix：By virgin soil soil 20-40%, river sand 5-15%, perlite 5-15%, baked wheaten cake soil 15-25%, pine tree bacterium
The weight ratio of root soil 30-40%, takes dry virgin soil soil, river sand, perlite, baked wheaten cake soil, is crushed to granularity less than or equal to after 4mm
It is uniformly mixed with loose pine tree mycorhiza soil, then it is 40-50% to adjust moisture content（I.e. plus water adjusts）, it is spare to obtain inoculation matrix；
（4）Make seedbed：The good greenhouse of moisturizing, insulation, irrigation condition is selected, seedbed is set in greenhouse, is stayed between seedbed
Pavement, then by step（3）The inoculation matrix prepared is tamped in seedbed；
（5）Seeding and seedling raising：Take step（1）In pretreated Huashan pine seed, be immersed in step（2）The India prepared
24-36h in ferfas strain liquid, taking-up are sown into step after drying（4）It is inoculated with matrix in the seedbed of making, is after planting spread above
The fine woods soil of one layer of lid, depth of planting 1-2cm；Temperature, humidity and illumination appropriate in greenhouse are kept, irregularly to seedbed
Watering, weeding, keep the moistening of inoculation matrix, its table soil is not split, until Huashan pine emergence is long to height of seedling 10-15cm, obtain
To Huashan loose seedling；
（6）Mycorrhiza fungi seeding cultivating：Step is taken out from seedbed（5）The Huashan loose seedling of cultivation, after every its main root that breaks, soaks again
Bubble is in step（2）3-5min is inoculated with the India truffle strain liquid prepared, is transplanted after taking-up in greenhouse, uses step
（3）The inoculation matrix single plant prepared is packed into seedling, keeps temperature, humidity and illumination appropriate in greenhouse, watered,
Loosen the soil, weeding and fertilising seedling management, inoculation soil humidity 40-60% is kept, until Huashan loose seedling length is to height of seedling 100-
120cm, that is, obtain India truffle mycorrhiza fungi seeding.
The step（5）In, after seed germination, monthly step of applying（2）The ferfas strain liquid of preparation 2-3 times, until China
Illawarra mountain pine emergence grows up to seedling, to strengthen the effect of inoculation of India truffle.
The step（3）The pH value of the inoculation matrix of preparation is 6.7-7.5（Adjusted using conventional method）, be preferably it is slightly sour extremely
Neutrality, in favor of the growth of the emergence of Huashan pine seed, growth and strain.
The pine tree mycorhiza soil that inoculation matrix is prepared in the step 1.3 is taken from being associated with the Huashan of India truffle in pine forest
Topsoil under pine tree, to ensure well-grown after strain inoculation.Conifer nursery cannot lack VA Mycorrhizal Fungi, such as lack pine tree bacterium
, may undergrowth after inoculation when root is native.
The step（4）The length and width in middle seedbed, it is deep be respectively 2-3m, 1-1.5m, 1-1.5m, the wide 40- of pavement between seedbed
50cm。
The step（5）The sowing time of middle Huashan pine seed preferably in the annual 3-4 months, with improve the emergence rate of seed and
The effect of inoculation；Thickness of sowing is every square metre 250-300.
The step（5）The fine woods soil of middle bedding is the topsoil in common Pinus armandii mixed forest, after sterilized sterilizing, powder
It is broken to that granularity 2mm is used below, the general thickness 5-10mm of fine woods soil of institute's bedding.
The step（5）With（6）In, in greenhouse temperature be 25-30 DEG C, humidity 75-85%, illuminance be
25000-30000lux。
The step（5）In, weeding follow except it is early, except it is small, except principle, accomplish bed surface and pavement without weeds, weeding
When prevent from loosening seedling root, watering is early every time, evening carries out and irrigates.
The step（5）In, Huashan loose seedling grows to 5 centimetres or so and starts thinning, fills the gaps with seedlings, and is carried out in rainy days, thinning,
Water immediately after filling the gaps with seedlings.
Each parameters such as composition proportion, the inoculation time of matrix are inoculated with the present invention can be specific definite according to being actually needed,
Ensure good effect of inoculation.
The present invention utilizes special inoculation nursery side using the inoculation matrix with India truffle strain and appropriate component
Method, by the multiple strain charity of too many levels, for India truffle strain, in the place of pine tree sapling root, good ring has been built in life
Border, substantially increases the success rate of inoculation, reduces the cost of artificial growth India truffle, can reach the rate of vaccination of India truffle
To more than 87%.There is method is reliable, rate of vaccination is high, cost is low etc., be suitably widely popularized use.
Embodiment
The embodiment of the present invention is further described in detail below.The technology being not specified in embodiment or production
Product are the prior art or can be by buying the conventional products obtained.
Embodiment 1：The method that this Huashan pine seed seedling-raising is inoculated with India truffle, it is comprised the following steps that：
（1）Seeds preprocess：High-quality Huashan pine seed is taken, it is clean with sterile water wash, it is then placed in the carbendazim of concentration 0.05%
Middle immersion 30min carries out disinfection sterilization treatment, with aseptic water washing 2 times after taking-up, is placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, is first cleaned up with tap water, is then rushed with sterile water
Wash 3 times and be placed on the smalls shape that 80 mesh of granularity is crushed in pulverizer, then by 1：10 volume ratio adds sterile water, obtains India
Ferfas strain liquid is spare；
（3）Prepare inoculation matrix：By virgin soil soil 20%, river sand 15%, perlite 15%, the weight for burning soil 20%, pine tree mycorhiza soil 30%
Ratio is measured, dry virgin soil soil, river sand, perlite, baked wheaten cake soil is taken, is associated with after being crushed to below granularity 2mm with being derived from pine forest
The Huashan pine tree layer soil of India truffle loose pine tree mycorhiza soil be uniformly mixed, then adjust moisture content be 40%, pH value be
6.7, it is spare to obtain inoculation matrix；
（4）Make seedbed：The good greenhouse of moisturizing, insulation, irrigation condition is selected, by the pavement of 40cm wide, is spaced in greenhouse
It is middle that length and width, the seedbed of deep respectively 2m, 1m, 1m are set, then by step（3）The inoculation matrix prepared is tamped in seedbed；
（5）Seeding and seedling raising：In annual March, take step（1）In pretreated Huashan pine seed, be immersed in step（2）System
24h in the India truffle strain liquid got ready, after taking-up is dried, step is sown into by the thickness of sowing of 250 every square metre（4）Make
Seedbed in be inoculated with matrix, after planting one thickness 10mm of bedding, sterilized sterilizing and be crushed to the Huashan pine of 1.5mm above
The fine woods soil in top layer, depth of planting 1cm in woods；25 DEG C of temperature, humidity 75%, quantity of illumination 30000lux in greenhouse are controlled, no
Periodically to seedbed watering, weeding, the moistening of inoculation matrix is kept, its table soil is not split, after seed germination, monthly step of applying
（2）The ferfas strain liquid of preparation 2 times, until Huashan pine emergence is long to height of seedling 10cm, obtains Huashan loose seedling；Weeding, which follows, to remove
It is early, except it is small, except principle, accomplish bed surface and pavement without weeds, when weeding prevents from loosening seedling root, and watering is early every time, evening carries out
And irrigate；Seedling grows to 5 centimetres or so and starts thinning, fills the gaps with seedlings, and is carried out in rainy days, thinning, water immediately after filling the gaps with seedlings；
（6）Mycorrhiza fungi seeding cultivating：Step is taken out from seedbed（5）The Huashan loose seedling of cultivation, after every its main root that breaks, soaks again
Bubble is in step（2）3min is inoculated with the India truffle strain liquid prepared, is transplanted after taking-up in greenhouse, uses step（3）
The inoculation matrix single plant prepared is packed into seedling, controls 25 DEG C of temperature, humidity 75%, illuminance 30000lux in greenhouse, into
Row waters, loosens the soil, weeding and fertilising seedling management, holding are inoculated with soil humidity 40%, up to Huashan loose seedling length to height of seedling
100cm, that is, obtain India truffle mycorrhiza fungi seeding.
For the present embodiment by using the method for the present invention, the rate of vaccination of India truffle is 82%, enters harvest within the 8th year after plantation
Phase, the per unit area yield of India truffle brings up to 8kg/ mus by the 3.5kg/ mus of existing method, and ferfas promotes the growth hair of Huashan pine
Educate, while Huashan pine effectively promotes the diseases and insect pests resistance of the growth and development raising plant of ferfas again, can keep with stablizing
The ecosystem, promotes the recovery and development of forest cover.
Embodiment 2：The method that this Huashan pine seed seedling-raising is inoculated with India truffle, it is comprised the following steps that：
（1）Seeds preprocess：High-quality Huashan pine seed is taken, it is clean with sterile water wash, it is then placed in the carbendazim of concentration 0.1%
Middle immersion 15min carries out disinfection sterilization treatment, with aseptic water washing 3 times after taking-up, is placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, is first cleaned up with tap water, is then rushed with sterile water
Wash 2 times and be placed on the smalls shape that 50 mesh of granularity is crushed in pulverizer, then by 1：5 volume ratio adds sterile water, obtains India
Ferfas strain liquid is spare；
（3）Prepare inoculation matrix：By virgin soil soil 40%, river sand 5%, perlite 5%, the weight for burning soil 20%, pine tree mycorhiza soil 30%
Than taking dry virgin soil soil, river sand, perlite, baked wheaten cake soil, print being associated with being derived from pine forest after being crushed to below granularity 4mm
Spend ferfas Huashan pine tree layer soil loose pine tree mycorhiza soil be uniformly mixed, then adjust moisture content be 50%, pH value be
7.5, it is spare to obtain inoculation matrix；
（4）Make seedbed：The good greenhouse of moisturizing, insulation, irrigation condition is selected, by the pavement of 50cm wide, is spaced in greenhouse
It is middle that length and width, the seedbed of deep respectively 3m, 1.5m, 1.5m are set, then by step（3）The inoculation matrix prepared is tamped in seedling
In bed；
（5）Seeding and seedling raising：In annual April, take step（1）In pretreated Huashan pine seed, be immersed in step（2）System
36h in the India truffle strain liquid got ready, after taking-up is dried, step is sown into by the thickness of sowing of 300 every square metre（4）Make
Seedbed in be inoculated with matrix, after planting one thickness 5mm of bedding, sterilized sterilizing and be crushed to the Pinus armandii mixed forest of 2mm above
The middle fine woods soil in top layer, depth of planting 2cm；30 DEG C of temperature, humidity 85%, quantity of illumination 25000lux in greenhouse are controlled, it is indefinite
Phase waters seedbed, weeding, keeps the moistening of inoculation matrix, its table soil is not split, after seed germination, monthly step of applying
（2）The ferfas strain liquid of preparation 3 times, until Huashan pine emergence is long to height of seedling 15cm, obtains Huashan loose seedling；Weeding, which follows, to remove
It is early, except it is small, except principle, accomplish bed surface and pavement without weeds, when weeding prevents from loosening seedling root, and watering is early every time, evening carries out
And irrigate；Seedling grows to 5 centimetres or so and starts thinning, fills the gaps with seedlings, and is carried out in rainy days, thinning, water immediately after filling the gaps with seedlings；
（6）Mycorrhiza fungi seeding cultivating：Step is taken out from seedbed（5）The Huashan loose seedling of cultivation, after every its main root that breaks, soaks again
Bubble is in step（2）5min is inoculated with the India truffle strain liquid prepared, is transplanted after taking-up in greenhouse, uses step（3）
The inoculation matrix single plant prepared is packed into seedling, controls 30 DEG C of temperature, humidity 85%, illuminance 28000lux in greenhouse, into
Row waters, loosens the soil, weeding and fertilising seedling management, holding are inoculated with soil humidity 60%, up to Huashan loose seedling length to height of seedling
120cm, that is, obtain India truffle mycorrhiza fungi seeding.
For the present embodiment by using the method for the present invention, the rate of vaccination of India truffle is 90%, enters harvest within the 5th year after plantation
Phase, the per unit area yield of India truffle brings up to 14kg/ mus by the 3.5kg/ mus of existing method, and ferfas promotes the growth hair of Huashan pine
Educate, while Huashan pine effectively promotes the diseases and insect pests resistance of the growth and development raising plant of ferfas again, can keep with stablizing
The ecosystem, promotes the recovery and development of forest cover.
Embodiment 3：The method that this Huashan pine seed seedling-raising is inoculated with India truffle, it is comprised the following steps that：
（1）Seeds preprocess：High-quality Huashan pine seed is taken, it is clean with sterile water wash, it is then placed in the carbendazim of concentration 0.08%
Middle immersion 20min carries out disinfection sterilization treatment, with aseptic water washing 3 times after taking-up, is placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, is first cleaned up with tap water, is then rushed with sterile water
Wash 2 times and be placed on the smalls shape that 60 mesh of granularity is crushed in pulverizer, then by 1：7 volume ratio adds sterile water, obtains India
Ferfas strain liquid is spare；
（3）Prepare inoculation matrix：By virgin soil soil 30%, river sand 10%, perlite 10%, the weight for burning soil 15%, pine tree mycorhiza soil 35%
Ratio is measured, dry virgin soil soil, river sand, perlite, baked wheaten cake soil is taken, is associated with after being crushed to below granularity 3mm with being derived from pine forest
The Huashan pine tree layer soil of India truffle loose pine tree mycorhiza soil be uniformly mixed, then adjust moisture content be 45%, pH value be
6.8, it is spare to obtain inoculation matrix；
（4）Make seedbed：The good greenhouse of moisturizing, insulation, irrigation condition is selected, by the pavement of 45cm wide, is spaced in greenhouse
It is middle that length and width, the seedbed of deep respectively 2.3m, 1.2m, 1.3m are set, then by step（3）The inoculation matrix prepared is tamped
In seedbed；
（5）Seeding and seedling raising：In annual April, take step（1）In pretreated Huashan pine seed, be immersed in step（2）System
30h in the India truffle strain liquid got ready, after taking-up is dried, step is sown into by the thickness of sowing of 280 every square metre（4）Make
Seedbed in be inoculated with matrix, after planting one thickness 8mm of bedding, sterilized sterilizing and be crushed to the Huashan pine of 1.8mm above
The fine woods soil in top layer, depth of planting 1.5cm in woods；28 DEG C of temperature, humidity 80%, quantity of illumination 25000lux in greenhouse are controlled,
Irregularly to seedbed watering, weeding, the moistening of inoculation matrix is kept, its table soil is not split, after seed germination, monthly apply step
Suddenly（2）The ferfas strain liquid of preparation 3 times, until Huashan pine emergence is long to height of seedling 12cm, obtains Huashan loose seedling；Weeding, which follows, to remove
It is early, except it is small, except principle, accomplish bed surface and pavement without weeds, when weeding prevents from loosening seedling root, and watering is early every time, evening carries out
And irrigate；Seedling grows to 5 centimetres or so and starts thinning, fills the gaps with seedlings, and is carried out in rainy days, thinning, water immediately after filling the gaps with seedlings；
（6）Mycorrhiza fungi seeding cultivating：Step is taken out from seedbed（5）The Huashan loose seedling of cultivation, after every its main root that breaks, soaks again
Bubble is in step（2）4min is inoculated with the India truffle strain liquid prepared, is transplanted after taking-up in greenhouse, uses step（3）
The inoculation matrix single plant prepared is packed into seedling, controls 28 DEG C of temperature, humidity 80%, illuminance 25000lux in greenhouse, into
Row waters, loosens the soil, weeding and fertilising seedling management, holding are inoculated with soil humidity 50%, up to Huashan loose seedling length to height of seedling
105cm, that is, obtain India truffle mycorrhiza fungi seeding.
For the present embodiment by using the method for the present invention, the rate of vaccination of India truffle is 87%, enters harvest within the 6th year after plantation
Phase, the per unit area yield of India truffle brings up to 12kg/ mus by the 3.5kg/ mus of existing method, and ferfas promotes the growth hair of Huashan pine
Educate, while Huashan pine effectively promotes the diseases and insect pests resistance of the growth and development raising plant of ferfas again, can keep with stablizing
The ecosystem, promotes the recovery and development of forest cover.
Embodiment 4：The method that this Huashan pine seed seedling-raising is inoculated with India truffle, it is comprised the following steps that：
（1）Seeds preprocess：High-quality Huashan pine seed is taken, it is clean with sterile water wash, it is then placed in the carbendazim of concentration 0.06%
Middle immersion 25min carries out disinfection sterilization treatment, with aseptic water washing 3 times after taking-up, is placed in spare in culture dish；
（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, is first cleaned up with tap water, is then rushed with sterile water
Wash 3 times and be placed on the smalls shape that 70 mesh of granularity is crushed in pulverizer, then by 1：8 volume ratio adds sterile water, obtains India
Ferfas strain liquid is spare；
（3）Prepare inoculation matrix：By virgin soil soil 24%, river sand 13%, perlite 12%, the weight for burning soil 18%, pine tree mycorhiza soil 33%
Ratio is measured, dry virgin soil soil, river sand, perlite, baked wheaten cake soil are taken, with being derived from association in pine forest after being crushed to below granularity 2.5mm
The loose pine tree mycorhiza soil for having the Huashan pine tree layer soil of India truffle is uniformly mixed, then adjusts moisture content as 43%, pH value
For 6.9, it is spare to obtain inoculation matrix；
（4）Make seedbed：The good greenhouse of moisturizing, insulation, irrigation condition is selected, by the pavement of 50cm wide, is spaced in greenhouse
It is middle that length and width, the seedbed of deep respectively 3m, 1.5m, 1.5m are set, then by step（3）The inoculation matrix prepared is tamped in seedling
In bed；
（5）Seeding and seedling raising：In annual April, take step（1）In pretreated Huashan pine seed, be immersed in step（2）System
32h in the India truffle strain liquid got ready, after taking-up is dried, step is sown into by the thickness of sowing of 260 every square metre（4）Make
Seedbed in be inoculated with matrix, after planting one thickness 7mm of bedding, sterilized sterilizing and be crushed to the Huashan pine of 1.6mm above
The fine woods soil in top layer, depth of planting 1.6cm in woods；26 DEG C of temperature, humidity 80%, quantity of illumination 26000lux in greenhouse are controlled,
Irregularly to seedbed watering, weeding, the moistening of inoculation matrix is kept, its table soil is not split, after seed germination, monthly apply step
Suddenly（2）The ferfas strain liquid of preparation 3 times, until Huashan pine emergence is long to height of seedling 11cm, obtains Huashan loose seedling；Weeding, which follows, to remove
It is early, except it is small, except principle, accomplish bed surface and pavement without weeds, when weeding prevents from loosening seedling root, and watering is early every time, evening carries out
And irrigate；Seedling grows to 5 centimetres or so and starts thinning, fills the gaps with seedlings, and is carried out in rainy days, thinning, water immediately after filling the gaps with seedlings；
（6）Mycorrhiza fungi seeding cultivating：Step is taken out from seedbed（5）The Huashan loose seedling of cultivation, after every its main root that breaks, soaks again
Bubble is in step（2）4min is inoculated with the India truffle strain liquid prepared, is transplanted after taking-up in greenhouse, uses step（3）
The inoculation matrix single plant prepared is packed into seedling, controls 27 DEG C of temperature, humidity 80%, illuminance 26000lux in greenhouse, into
Row waters, loosens the soil, weeding and fertilising seedling management, holding are inoculated with soil humidity 43%, up to Huashan loose seedling length to height of seedling
105cm, that is, obtain India truffle mycorrhiza fungi seeding.
For the present embodiment by using the method for the present invention, the rate of vaccination of India truffle is 82%, enters harvest within the 7th year after plantation
Phase, the per unit area yield of India truffle brings up to 10kg/ mus by the 3.5kg/ mus of existing method, and ferfas promotes the growth hair of Huashan pine
Educate, while Huashan pine effectively promotes the diseases and insect pests resistance of the growth and development raising plant of ferfas again, can keep with stablizing
The ecosystem, promotes the recovery and development of forest cover.
The technology contents of the present invention are described above, but protection scope of the present invention is not limited to the content,
, can also be on the premise of present inventive concept not be departed to this hair in the knowledge that those of ordinary skill in the art possess
Bright technology contents make a variety of changes, within the spirit and principles of the invention, any modification for being made, equivalent substitution,
Improve etc., it should all be included in the protection scope of the present invention.
Claims (10)
- A kind of 1. method of Huashan pine seed seedling-raising inoculation India truffle, it is characterised in that comprise the following steps that：（1）Seeds preprocess：High-quality Huashan pine seed is taken, it is clean with sterile water wash, it is then placed in the more of concentration 0.05-0.1% 15-30min is soaked in bacterium spirit to carry out disinfection sterilization treatment, with aseptic water washing 2-3 times after taking-up, is placed in spare in culture dish；（2）Prepare ferfas strain liquid：Ripe fresh India truffle is selected, is first cleaned up with tap water, is then rushed with sterile water Wash 2-3 times and be placed on the smalls shape that granularity 50-80 mesh is crushed in pulverizer, then by 1：The volume ratio of 5-10 adds sterile water, It is spare to obtain India truffle strain liquid；（3）Prepare inoculation matrix：By virgin soil soil 20-40%, river sand 5-15%, perlite 5-15%, baked wheaten cake soil 15-25%, mycorhiza soil The weight ratio of 30-40%, takes dry virgin soil soil, river sand, perlite, burns soil, be crushed to granularity less than or equal to after 4mm with pine Scattered pine tree mycorhiza soil is uniformly mixed, then it is 40-50% to adjust moisture content, and it is spare to obtain inoculation matrix；（4）Make seedbed：The good greenhouse of moisturizing, insulation, irrigation condition is selected, seedbed is set in greenhouse, is stayed between seedbed Pavement, then by step（3）The inoculation matrix prepared is tamped in seedbed；（5）Seeding and seedling raising：Take step（1）In pretreated Huashan pine seed, be immersed in step（2）The India prepared 24-36h in ferfas strain liquid, taking-up are sown into step after drying（4）It is inoculated with matrix in the seedbed of making, is after planting spread above The fine woods soil of one layer of lid, depth of planting 1-2cm；Temperature, humidity and illumination appropriate in greenhouse are kept, irregularly to seedbed Watering, weeding, keep the moistening of inoculation matrix, its table soil is not split, until Huashan pine emergence is long to height of seedling 10-15cm, obtain To Huashan loose seedling；（6）Mycorrhiza fungi seeding cultivating：Step is taken out from seedbed（5）The Huashan loose seedling of cultivation, after every its main root that breaks, soaks again Bubble is in step（2）3-5min is inoculated with the India truffle strain liquid prepared, is transplanted after taking-up in greenhouse, uses step （3）The inoculation matrix single plant prepared is packed into seedling, keeps temperature, humidity and illumination appropriate in greenhouse, watered, Loosen the soil, weeding and fertilising seedling management, inoculation soil humidity 40-60% is kept, until Huashan loose seedling length is to height of seedling 100- 120cm, that is, obtain India truffle mycorrhiza fungi seeding.
- 2. the method for Huashan pine seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step （5）In, after seed germination, monthly step of applying（2）The ferfas strain liquid of preparation 2-3 times, until Huashan pine emergence grows up to seedling.
- 3. the method for Huashan pine seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step （3）The pH value of the inoculation matrix of preparation is 6.7-7.5, is preferably slightly sour to neutral.
- 4. the method for Huashan pine seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step （3）In, the pine tree mycorhiza soil for preparing inoculation matrix is taken from the topsoil being associated with pine forest under the Huashan pine tree of India truffle Earth.
- 5. the method for Huashan pine seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step （4）In, the length and width in seedbed, it is deep be respectively 2-3m, 1-1.5m, 1-1.5m, the wide 40-50cm of pavement between seedbed.
- 6. the method for Huashan pine seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step （5）In, the sowing time of Huashan pine seed, thickness of sowing was every square metre 250-300 preferably in the annual 3-4 months.
- 7. the method for Huashan pine seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step （5）In, after planting the fine woods soil of bedding is the topsoil in common Pinus armandii mixed forest, and granularity is crushed to after sterilized sterilizing 2mm is used below, the fine woods soil layer generally thickness 5-10mm of institute's bedding.
- 8. the method for Huashan pine seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step （5）With（6）In, in greenhouse temperature be 25-30 DEG C, humidity 75-85%, illuminance 25000-30000lux.
- 9. the method for Huashan pine seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step （5）In, weeding follow except it is early, except it is small, except principle, accomplish bed surface and pavement without weeds, when weeding prevents from loosening seedling root, often Secondary watering is early, evening carries out and irrigates.
- 10. the method for Huashan pine seed seedling-raising inoculation India truffle according to claim 1, it is characterised in that：The step Suddenly（5）In, Huashan loose seedling grows to 5 centimetres or so and starts thinning, fills the gaps with seedlings, and is carried out in rainy days, thinning, pour immediately after filling the gaps with seedlings Water.
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