CN107969182A - It is a kind of to have ecological method that is disease-resistant and reducing the place greenhouse gas emissions such as farm and application concurrently - Google Patents
It is a kind of to have ecological method that is disease-resistant and reducing the place greenhouse gas emissions such as farm and application concurrently Download PDFInfo
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- CN107969182A CN107969182A CN201710856145.5A CN201710856145A CN107969182A CN 107969182 A CN107969182 A CN 107969182A CN 201710856145 A CN201710856145 A CN 201710856145A CN 107969182 A CN107969182 A CN 107969182A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/80—Penicillium
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/885—Trichoderma
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Abstract
A kind of to have ecological method and its application for suppressing soil germ and reducing the place greenhouse gas emissions such as farm concurrently, which belongs to agricultural, animal husbandry, municipal refuse landfill, environmental protection and reduction of greenhouse gas discharge field.The technology is realized by following steps：One kind can be suppressed into the place greenhouse gas emission such as farm, Organic Fertilizer Plants, garbage stacking yard and refuse landfill (CH4, CO2, N2O microbial fermentation), zymotic fluid or solid fermentation product are used in farm and Organic Fertilizer Plants feces of livestock and poultry, or in garbage stacking yard or landfill yard rubbish, stir stirring feces of livestock and poultry or rubbish, mix.The active ingredient secreted by microbial strains suppresses soil and feces of livestock and poultry cellulase, amylase, nitrate reductase, protease, the microbial activity such as nitric oxidereductase and nitrification, denitrification, methanogen, cellulose-decomposing bacteria, reduce soil or feces of livestock and poultry or organic matter in garbage and ammonium nitrogen, nitrate nitrogen are converted into farmland greenhouse gas, achieve the purpose that reduction of greenhouse gas discharge, reduce feces of livestock and poultry nutrient loss, it can suppress the harmful microorganism in soil at the same time, promote the growth of crop, Ecological Sustainable Development.
Description
Technical field
The invention belongs to agricultural, animal husbandry, garbage loading embeading and field of environment protection, more particularly to one kind has concurrently disease-resistant and subtracts
The ecological method of the place greenhouse gas emissions such as few farm and application.
Background technology
Climate warming caused by Global climate change, from greenhouse effects, is also known as " greenhouse effect ", in glass room too
After positive direct projection enters, temperature raises rapidly, even if keeping the sun off, higher temperature is still maintained, at this moment due to by sunlight heats
Air launch long-wave radiation, so as to discharge heat, raises temperature in glass room, is being commonly called as air insulation effect.Air can make
Solar shortwave radiation reaches ground, but the long wave caloradiance outwards released of earth's surface is by Atmospheric Absorption, thus make earth's surface with
Lower atmosphere layer temperature increases, because its effect is similar to the greenhouse of crop culture, therefore named greenhouse effects.Since the industrial revolution,
The strong greenhouse gases of the heat absorptivities such as the carbon dioxide that the mankind are discharged into air increase year by year, and the greenhouse effects of air also increase therewith
By force, cause a series of serious problems such as global warming, cause the concern of whole world various countries.
Office's estimation, China annual about 6,000,000,000 tons of CO of agricultural emissions greenhouse gases at present2Equivalent, accounts for No. 1 in the world, about
The 30% of Global Agriculture discharge is accounted for, wherein, CH4Account for 25%, the N always discharged2O accounts for 52%, the CO always discharged2Account for what is always discharged
23%.By sources analyze, Rice Cropping discharges 14264.45 ten thousand tons, accounts for 9%；Husbandry sector discharges 42709.94 ten thousand tons, accounts for
26.94%；47457.81 ten thousand tons of soil discharge, accounts for 29.93%；Discharge caused by the input such as chemical fertilizer, the energy, pesticide, agricultural film
54125.11 ten thousand tons, account for 34.14%.
Greenhouse gases acknowledged at present have six major classes, carbon dioxide, methane, nitrous oxide, carbon fluorohydrocarbon, water, ammonia
Deng, wherein carbon dioxide, methane, nitrous oxide mainly with agricultural and farming activities it is related.Carbon dioxide (CO2) it is to remain in agriculture
Crop material, spoil and the organic matter such as organic fertilizer, the feces of livestock and poultry of farm in the soil of field pass through all kinds of oxygen in soil
Change the product after the aerobic or anaerobic oxidation of microorganism；Nitrous oxide (N2O it is) that content is only second to CO in air2And CH4It is important
Greenhouse gases, are mainly derived from the nitrification of nitrogen, nitrosification and motor vehicle exhaust emission, lightning in soil and feces of livestock and poultry
Deng；N2O can also absorb infrared ray strongly, reduce earth's surface by heat radiation of the air to outer space, so that cause greenhouse effects, it is right
Global environment and climate change have important influence.N2O properties in troposphere are stablized, and it is huge to heat potentiality, its greenhouse effect
It should be 290-310 times of same concentration carbon dioxide；Methane (CH4) it is one of main greenhouse gas, its contribution to global warming
Account for 20%~39% (IPCC, 2007).CH in air4Content before the industrial revolution (1750) 0.72 μm of ol
mol-1Rise to 1.77 μm of olmol of 2005-1, increase about 2.5 times, and CH4Molecule has very strong infrared absorption
Ability, it is CO that its unimolecule, which heats potentiality,219-25 times (IPCC, 2007), therefore CH4It is considered as after CO2It is most important afterwards
One of greenhouse gases, it is produced, consumption and emission mechanism are still the hot issue studied at present.In soil anaerobic environment
In, organic substance and the soil organism based on water plant root exudates and cast pass through under methanogen effect
Anaerobic fermentation produces CH4, so wetland becomes one of Major Natural approach of greenhouse gas methane discharge.
Methane is occurred mainly in the rice field of waterflooding, and main farmland greenhouse gas is carbon dioxide and oxygen in upland soil
Change sub- nitrogen, wherein nitrous oxide is the main source of greenhouse gases nitrous oxide, is passed through mainly due to agricultural application excess nitrogenous fertilizer
Caused by the oxidation or reduction of nitrifying microorganisms and denitrifying microorganism.
A large amount of greenhouse gases can be all produced during livestock and poultry farm excrement, refuse landfill, organic fertilizer production are long, study agriculture
Woods animal husbandry and city refuse landfill Technology of Reducing Greenhouse Gas Emissions particularly farm's feces of livestock and poultry, organic fertilizer production, rubbish
Isothermal chamber gas abatement technology is filled, new technology and method will be provided for agriculture level city reduction of greenhouse gas discharge, be that the mankind can
Sustainable development provides safeguard.
The content of the invention
The technical problem of solution：The present invention is asked for aquaculture, organic fertilizer production, the discharge of garbage loading embeading isothermal chamber gas
Topic, develops a kind of simple and practicable method, there is provided and one kind can reduce these place greenhouse gas emission biological methods and its application,
This method is that first trichoderma asperellum and mould ferment, and then mixing leaching, is made product, in agricultural land soil or feces of livestock and poultry or rubbish
The mix bacterium agent is sprayed in rubbish landfill yard or organic fertilizer production, can greatly reduce farm's feces of livestock and poultry or organic fertilizer production or
Greenhouse gas emission in refuse landfill, can also reduce farmland and/or feces of livestock and poultry greenhouse during whole plant growth
The discharge of gas, while soil-borne disease is reduced, soil productivity is improved, improves crop quality, increases peasant economy benefit.
Technical solution：It is a kind of to have ecological method that is disease-resistant and reducing the place greenhouse gas emissions such as farm and application concurrently,
Step is：
A. 1-20 kilograms of trichoderma asperellum is inoculated into 1000 liters of sterile mediums or 1000 kilograms of sterilizing solid mediums (is used
Ordinary tap water or clean river water when exposure 24 is small in advance are prepared) in, every 1000 liters of nutrient solutions or the training of every 1000 kilograms of solids
Foster base contains：Soluble starch 2-50.0kg, 1.5-15 kilograms of glucose, NaCl 0.01-0.30kg, K2HPO4·3H2O
0.03-0.3kg, FeSO4·7H2O 0.01-0.05kg, MgSO4·7H2O 0.05-0.2kg, pH value 4.2-7.5.Start hair
Fermentation tank agitating shaft, and ventilate, throughput 0.2-0.6V/V.M (every cubic metre of zymotic fluid per minute is ventilated 0.2-0.6 cubic metres),
When 28 degree of continuous culture 96-120 are small, terminate, warping is spare；Or 1000 kilograms of sterilizing solid mediums are spread prior
1-10 meters long in the shallow pond of indoor cement of sterilization, 1-5 meters wide, 5-20 centimetres of culture medium thickness, keeps interior 20-30 degree,
Solid medium water content 40-60%, with the moisturizing of plastic covering film thermal insulating and can prevent from polluting, be punched on film above,
Every square metre 5-15, aperture 2-8 millimeters, stirred and mix once every 4 days halfway, continuously ferment 8-14 days, terminate, collect standby
With；B. 1-20 kilograms of mould is inoculated into 1000 liters of sterile mediums or 1000 kilograms of sterilizing solid mediums (with exposure in advance
Ordinary tap water or clean river water when air 24 is small are prepared) in, every 1000 liters of nutrient solutions or every 1000 kilograms of solid mediums
Contain：Soluble starch 2-80.0kg, 1.5-20 kilograms of glucose, NaCl 0.01-0.40kg, K2HPO4·3H2O 0.03-
0.4kg, FeSO4·7H2O 0.01-0.06kg, MgSO4·7H2O 0.05-0.3kg, pH value 4.0-7.0.Start fermentation tank
Agitating shaft, and ventilate, throughput 0.2-0.6V/V.M (every cubic metre of zymotic fluid per minute is ventilated 0.2-0.6 cubic metres), 28 degree
When continuously culture 96-120 is small, terminate, warping is spare；Or 1000 kilograms of sterilizing solid mediums are spread and are sterilized in advance
1-15 meters long in the shallow pond of indoor cement of sterilizing, 1-8 meters wide, 5-25 centimetres of culture medium thickness, keeps interior 20-30 degree, solid
Moisture content in medium 40-60%, with the moisturizing of plastic covering film thermal insulating and can prevent from polluting, punch on film, often put down above
Square rice 5-15, aperture 2-8 millimeters, stirred and mix once every 4 days halfway, continuously ferment 8-14 days, terminate, collect spare；
C. as needed, by concentrating or diluting above-mentioned a, in b zymotic fluids or in solid fermentation matrix, spore quantity living, which controls, exists
Every milliliter/or 0.5 hundred million -10 hundred million every gram；Respectively by the zymotic fluid in a and b after fermentation or solid fermentation matrix by quality/
Mass ratio a: b=0.5-1: 0.5-1 is mixed, thoroughly mix, product；D. it is formal in use, feces of livestock and poultry according to
The above-mentioned 1-8 kilograms of mix bacterium agent of c products of 1000 kilograms of administrations of fresh excreta, is uniformly mixed；Household refuse landfill sites press life rubbish
1000 kilograms of rubbish uses 0.5-5 kilograms of mix bacterium agent, is uniformly mixed；For farmland especially continuous cropping obstacle to be serious and soil-borne disease
Serious soil, per acre using above-mentioned 2-4 kilograms of c products, uniformly sprays in agricultural land soil surface, ploughs soil immediately, or
It is appropriate at the same time to irrigate, long-term cropping；It can also be used for the ground such as riverine wetland, grassland, forest, it is possible to reduce soil and/or animal dung
Just isothermal chamber gas CH4、CO2And N2The formation and discharge of O.
Described can have concurrently suppresses soil germ and reduces the place greenhouse gas emission biological methods such as farm in farm
Application on feces of livestock and poultry, organic fertilizer production, refuse landfill, farmland and forest.
Trichoderma asperellum and mould can be at Institute of Micro-biology of the Chinese Academy of Sciences, national Culture Collection Center and applicants in this technology
Obtain.
Beneficial effect：The present invention can reduce farm's feces of livestock and poultry, organic fertilizer production, garbage loading embeading and farmland, sylvogenic soil
The discharge of earth greenhouse gases, promotes plant growth, reduces soil-borne disease of crop；Its maximum function be reduce agricultural animal husbandry and
The discharge of refuse landfill greenhouse gases.
The present invention can greatly reduce agricultural animal husbandry and refuse landfill greenhouse gases, be conducive to human kind sustainable development.
The present invention is compared with prior art：
1) place such as existing farm, organic fertilizer production, refuse landfill, considers the emission reduction of greenhouse gases, this hair very well
The place Technology of Reducing Greenhouse Gas Emissions such as the cultivation place of bright middle use, method is easy, it is technically simple it is easy, workable, into
This is cheap, highly practical；
2) Technology of Reducing Greenhouse Gas Emissions that the present invention uses, can pass through trichoderma asperellum and mould fermented liquid or solid fermentation
Middle antibacterial substance suppresses soil, feces of livestock and poultry cellulase, nitrification, nitrosation, denitrifying microorganism, methanogen in product
Activity, so as to suppress soil or feces of livestock and poultry or organic matter in garbage is converted into the speed of methane, carbon dioxide, nitrous oxide
And quantity, reduce feces of livestock and poultry nutrient loss；Both bacterium can also suppress some soil-borne disease bacterium, promote the work of plant growth at the same time
With raising soil productivity, promotes plant growth.This technology and product have the function that emission reduction and suppression soil-borne disease at the same time,
This is having not yet to see open report.
Brief description of the drawings
Fig. 1 is that pig manure uses greenhouse gas emission change schematic diagram during the technical products.Fig. 2 be cucumber growth it is long in
Use the droop incidence schematic diagram in the technical products
Embodiment
Below in conjunction with example, the invention will be further described：
Embodiment 1：
1.. 1 kilogram of trichoderma asperellum is inoculated into 1000 liters of sterile mediums or 1000 kilograms of sterilizing solid mediums, often
1000 liters of nutrient solutions contain：Soluble starch 2kg, 1.5 kilograms of glucose, NaCl 0.01kg, K2HPO4·3H2O 0.03kg,
FeSO4·7H2O 0.01kg, MgSO4·7H2O 0.05kg, pH value 4.2.Fermentation tank agitating shaft is started, and is ventilated, throughput
0.2V/V.M (per minute every cubic metre of zymotic fluid ventilate 0.2 cubic metre), when 28 degree continuous cultures 100 are small, end, warping is standby
With；
2. Penicillium notatum is inoculated into 1000 liters of sterile mediums for 1 kilogram, every 1000 liters of nutrient solutions contain：Soluble starch
2-80.0kg, 1.5 kilograms of glucose, NaCl 0.01kg, K2HPO4·3H2O 0.03kg, FeSO4·7H2O 0.01-0.06kg,
MgSO4·7H2O 0.05kg, pH value 4.5.Fermentation tank agitating shaft is started, and is ventilated, throughput 0.2V/V.M is (per minute often to stand
Square rice zymotic fluid is ventilated 0.2 cubic metre), when 28 degree continuous cultures 96 are small, end, warping is spare；
3. as needed, by concentrating or diluting spore living in above-mentioned Trichoderma asperellum and/or mould fermented liquid
Quantity is controlled at every milliliter/or 0.5 hundred million every gram；Respectively by the fermentation in Trichoderma asperellum and/or Penicillium notatum after fermentation
Liquid is mixed by mass/mass than Trichoderma asperellum/Penicillium notatum=0.5: 0.5, thoroughly mix, final products；
4. formal in use, feces of livestock and poultry is according to the 1 kilogram of mixing of the above-mentioned final products of 1000 kilograms of administrations of fresh excreta
Microbial inoculum, is uniformly mixed；Household refuse landfill sites press 1000 kilograms of house refuse, 0.5 kilogram of use mix bacterium agent, are uniformly mixed；With
In farmland especially continuous cropping obstacle is serious and the serious soil of soil-borne disease, per acre using 2 kilograms of the said goods, uniformly spray in
Agricultural land soil surface, ploughs soil, or appropriate irrigation at the same time, long-term cropping immediately；It can also be used for riverine wetland, grassland, gloomy
The ground such as woods, it is possible to reduce soil and/or feces of livestock and poultry isothermal chamber gas CH4、CO2And N2The formation and discharge of O.
Embodiment 2：
1.. by trichoderma asperellum 3 kilograms be inoculated into 1000 liters of sterile mediums, every 1000 liters of nutrient solutions contain：It is soluble
Starch 5kg, 2 kilograms of glucose, NaCl 0.01kg, K2HPO4·3H2O 0.3kg, FeSO4·7H2O 0.015kg, MgSO4·
7H2O0.1kg, pH value 4.5.Fermentation tank agitating shaft is started, and is ventilated, throughput 0.3V/V.M (every cubic metre of fermentation per minute
Liquid is ventilated 0.3 cubic metre), when 28 degree continuous cultures 110 are small, end, warping is spare；
2. Penicillium notatum is inoculated into 1000 kilograms of sterilizing solid mediums for 4 kilograms, every 1000 kilograms of solid mediums contain
Have：Soluble starch 8kg, 5 kilograms of glucose, NaCl 0.08kg, K2HPO4·3H2O 0.2kg, FeSO4·7H2O 0.02kg,
MgSO4·7H2O 0.05-0.3kg, pH value 5.0.1000 kilograms of sterilizing solid mediums are spread in prior sterilization
5 meters long in the indoor shallow pond of cement, 1 meter wide, 5 centimetres of culture medium thickness, keeps 25 degree indoor, solid medium water content 45%,
It with the moisturizing of plastic covering film thermal insulating and can prevent from polluting above, be punched on film, 8 every square metre, 5 millimeters of aperture, in
Way was stirred every 4 days mixes once, continuously ferments 10 days, terminates, and collects spare；
3. as needed, by concentrating or diluting in above-mentioned Trichoderma asperellum and/or Penicillium notatum solid fermentation matrix
Spore quantity control living is at every milliliter/or 200,000,000 every gram；Respectively by Trichoderma asperellum and/or Penicillium notatum after fermentation
Zymotic fluid or solid fermentation matrix are mixed by mass/mass than Trichoderma asperellum/Penicillium notatum=0.5: 0.8, are thoroughly mixed,
Final products；
4. formal in use, feces of livestock and poultry is according to the 2 kilograms of mixing of the above-mentioned final products of 1000 kilograms of administrations of fresh excreta
Microbial inoculum, is uniformly mixed；Household refuse landfill sites press 1000 kilograms of house refuse, 2 kilograms of use mix bacterium agent, are uniformly mixed；For
Farmland especially continuous cropping obstacle is serious and the serious soil of soil-borne disease, per acre using 3 kilograms of the said goods, uniformly sprays in agriculture
Field soil surface, ploughs soil, or appropriate irrigation at the same time, long-term cropping immediately；It can also be used for riverine wetland, grassland, forest
Etc. ground, it is possible to reduce soil and/or feces of livestock and poultry isothermal chamber gas CH4、CO2And N2The formation and discharge of O.
Embodiment 3
1.. 10 kilograms of trichoderma asperellum is inoculated into 1000 kilograms of sterilizing solid mediums, every 1000 kilograms of solid cultures
Base contains：Soluble starch 10.0kg, 6 kilograms of glucose, NaCl 0.2kg, K2HPO4·3H2O 0.1kg, FeSO4·7H2O
0.03kg, MgSO4·7H2O 0.09kg, pH value 5.1000 kilograms of sterilizing solid mediums are spread in prior sterilization
8 meters long in the indoor shallow pond of cement, 2 meters wide, 10 centimetres of culture medium thickness, keeps 22 degree indoor, solid medium water content
50%, it with the moisturizing of plastic covering film thermal insulating and can prevent from polluting above, be punched on film, 10 every square metre, the milli of aperture 6
Rice, stirred every 4 days and mixes once, continuously ferment 14 days halfway, terminated, and collected spare；
2. Penicillium notatum is inoculated into 1000 liters of sterile mediums 12 kilograms, every 1000 liters of nutrient solutions contain：Soluble starch
30kg, 12 kilograms of glucose, NaCl 0.40kg, K2HPO4·3H2O 0.2kg, FeSO4·7H2O 0.06kg, MgSO4·
7H2O0.3kg, pH value 6.Fermentation tank agitating shaft is started, and is ventilated, throughput 0.6V/V.M (every cubic metre of zymotic fluid per minute
0.6 cubic metre of ventilation), when 28 degree continuous cultures 120 are small, end, warping is spare；
3. as needed, by concentrating or diluting in above-mentioned Trichoderma asperellum and/or mould fermented liquid or solid
Spore quantity control living is at every milliliter/or 500,000,000 every gram in fermentation substrate；It will be sent out respectively in Trichoderma asperellum and/or Penicillium notatum
Zymotic fluid or solid fermentation matrix after ferment are mixed by mass/mass than Trichoderma asperellum/Penicillium notatum=1: 0.5,
Thoroughly mix, final products；
4. formal in use, feces of livestock and poultry is according to the 8 kilograms of mixing of the above-mentioned final products of 1000 kilograms of administrations of fresh excreta
Microbial inoculum, is uniformly mixed；Household refuse landfill sites press 1000 kilograms of house refuse, 4 kilograms of use mix bacterium agent, are uniformly mixed；For
Farmland especially continuous cropping obstacle is serious and the serious soil of soil-borne disease, per acre using 4 kilograms of the said goods, uniformly sprays in agriculture
Field soil surface, ploughs soil, or appropriate irrigation at the same time, long-term cropping immediately；It can also be used for riverine wetland, grassland, forest
Etc. ground, it is possible to reduce soil and/or feces of livestock and poultry isothermal chamber gas CH4、CO2And N2The formation and discharge of O.
The application example of the present invention：
The technology of the present invention is used for the application of corn, wheat and pig manure.
1st, with reference to embodiment 1, on 200 kilograms of fresh pig manures, using 1 kilogram of mix bacterium agent, adopted with plenum chamber within every two days
Collect gas sample and carry out GC analysis measure greenhouse gases contents, the control pig manure with not using this technology is compared, and discharge of methane is reduced
49%, CO2 emission reduces 62%, and nitrous oxide emission reduces by 66% (Fig. 1)；
2nd, with reference to embodiment 2, on potted plant cucumber soil, using 50 grams/basin mix bacterium agent, gathered with plenum chamber within every two days
Gas sample carries out GC analysis measure greenhouse gases contents, while the incidence of cucumber fusarium axysporum is observed after using 40 days, with not having
Compared using the control soil of this technology, discharge of methane reduces 36%, and CO2 emission reduces 53%, and nitrous oxide emission subtracts
Few 60%, cucumber fusarium axysporum incidence reduces by 57% (Fig. 2)；
3rd, with reference to embodiment 3, on 1000 kilograms of landfill wastes, using 3 kilograms of mix bacterium agents, adopted with plenum chamber within every two days
Collect gas sample and carry out GC analysis measure greenhouse gases contents, the control rubbish with not using this technology is compared, and discharge of methane is reduced
35%, CO2 emission reduces 53%, and nitrous oxide emission reduces 48%.
Claims (6)
1. a kind of have ecological method that is disease-resistant and reducing the place greenhouse gas emissions such as farm and application concurrently, it is characterised in that step
Suddenly it is：
A. by trichoderma asperellum (Trichoderma asperellum) 1-20 kilograms be inoculated into 1000 liters of sterile mediums or 1000
Kilogram sterilizing solid medium (with advance exposure 24 it is small when ordinary tap water or clean river water prepare) in, it is every 1000 liters culture
Liquid or every 1000 kilograms of solid mediums contain：Soluble starch 2-50.0kg, 1.5-15 kilograms of glucose, NaCl 0.01-
0.30kg, K2HPO4·3H2O 0.03-0.3kg, FeSO4·7H2O 0.01-0.05kg, MgSO4·7H2O 0.05-0.2kg,
PH value is 4.2-7.5.Fermentation tank agitating shaft is started, and is ventilated, throughput 0.2-0.6V/V.M (every cubic metre of zymotic fluid per minute
0.2-0.6 cubic metre of ventilation), 28-30 degree continuously cultivate 96-120 it is small when, end, warping is spare；Or 1000 kilograms are gone out
Bacterium solid medium is spread in the shallow pond of indoor cement of prior sterilization, 1-10 meters long, 1-5 meters wide, and culture medium material is thick
5-20 centimetres of degree, keeps interior 20-30 degree, solid medium water content 40-60%, can be protected with plastic covering film thermal insulating above
It is wet and prevent from polluting, punched on film, every square metre 5-15, aperture 2-8 millimeters, stirred and mix once every 4 days halfway,
Continuously ferment 8-14 days, terminate, collect spare；
B. 1-20 kilograms of mould (Penecilium sp.) is inoculated into 1000 liters of sterilized liquid culture mediums or 1000 kilograms of sterilizings
In solid medium (being prepared with ordinary tap water of the prior exposure air 24 when small or clean river water), every 1000 liters of nutrient solutions or
Every 1000 kilograms of solid mediums contain：Soluble starch 2-80.0kg, 1.5-20 kilograms of glucose, NaCl 0.01-
0.40kg, K2HPO4·3H2O 0.03-0.4kg, FeSO4·7H2O 0.01-0.06kg, MgSO4·7H2O 0.05-0.3kg,
PH value is 4.0-7.0.Fermentation tank agitating shaft is started, and is ventilated, throughput 0.2-0.6V/V.M (every cubic metre of zymotic fluid per minute
0.2-0.6 cubic metre of ventilation), 28-30 degree continuously cultivate 96-120 it is small when, end, warping is spare；Or 1000 kilograms are gone out
Bacterium solid medium is spread in the shallow pond of indoor cement of prior sterilization, 1-15 meters long, 1-8 meters wide, culture medium thickness 5-
25 centimetres, keep interior 20-30 degree, solid medium water content 40-60%, above can with the moisturizing of plastic covering film thermal insulating with
Prevent from polluting, punched on film, every square metre 5-15, aperture 2-8 millimeters, stirred and mix once every 4 days halfway, continuously
Fermentation 8-14 days, terminates, and collects spare；
C. as needed, by concentrating or diluting above-mentioned a, in b zymotic fluids or work spore quantity control in solid fermentation matrix
System is at every milliliter/or 0.5 hundred million -10 hundred million every gram；The zymotic fluid in a and b after fermentation or solid fermentation matrix are pressed respectively
Mass/mass is mixed than a: b=0.5-1: 0.5-1, thoroughly mix, product；
D. formal in use, feces of livestock and poultry is according to the above-mentioned 1-8 kilograms of mix bacterium agent of c products of 1000 kilograms of administrations of fresh excreta,
It is uniformly mixed；Household refuse landfill sites use 0.5-5 kilograms of mix bacterium agent for 1000 kilograms by house refuse, are uniformly mixed；For
Farmland especially continuous cropping obstacle is serious and the serious soil of soil-borne disease, per acre using above-mentioned 2-4 kilograms of c products, uniformly sprays
In agricultural land soil surface, soil, or appropriate irrigation at the same time, long-term cropping are ploughed immediately；Can also be used for riverine wetland, grassland,
The ground such as forest, it is possible to reduce soil and/or feces of livestock and poultry isothermal chamber gas CH4、CO2And N2The formation and discharge of O.
2. a kind of according to claim 1 have ecological method that is disease-resistant and reducing the place greenhouse gas emissions such as farm concurrently,
It is characterized in that the biological method is inoculated into 1000 liters of sterile mediums using 1-20 kilograms of trichoderma asperellum or 1000 kilograms go out
In bacterium solid medium (being prepared with ordinary tap water when exposure 24 is small in advance or clean river water), every 1000 liters of nutrient solutions or every
1000 kilograms of solid mediums contain：Soluble starch 2-50.0kg, 1.5-15 kilograms of glucose, NaCl 0.01-0.30kg,
K2HPO4·3H2O 0.03-0.3kg, FeSO4·7H2O 0.01-0.05kg, MgSO4·7H2O 0.05-0.2kg, pH value are
4.2-7.5.Fermentation tank agitating shaft is started, and is ventilated, throughput 0.2-0.6V/V.M (every cubic metre of zymotic fluid ventilations per minute
0.2-0.6 cubic metres), when 28 degree of continuous culture 96-120 are small, terminate, warping is spare；Or 1000 kilograms of sterilizing solids are trained
Foster base is spread in the shallow pond of indoor cement of prior sterilization, 1-10 meters long, 1-5 meters wide, 5-20 centimetres of culture medium thickness, is protected
Indoor 20-30 degree is held, solid medium water content 40-60%, with the moisturizing of plastic covering film thermal insulating and can prevent from polluting above,
In film perforating, every square metre 5-15, aperture 2-8 millimeters, stirred and mix once every 4 days halfway, continuously ferment 8-14 days,
Terminate, collect spare；1-20 kilograms of mould is inoculated into 1000 liters of sterile mediums or 1000 kilograms of sterilizing solid cultures at the same time
In base (being prepared with ordinary tap water of the prior exposure air 24 when small or clean river water), every 1000 liters of nutrient solutions or every 1,000,000
Gram solid medium contains：Soluble starch 2-80.0kg, 1.5-20 kilograms of glucose, NaCl 0.01-0.40kg, K2HPO4·
3H2O 0.03-0.4kg, FeSO4·7H2O 0.01-0.06kg, MgSO4·7H2O0.05-0.3kg, pH value 4.0-7.0.Open
Dynamic fermentation tank agitating shaft, and ventilate, throughput 0.2-0.6V/V.M (ventilate 0.2-0.6 cubes by every cubic metre of zymotic fluid per minute
Rice), when 28 degree continuous culture 96-120 are small, end, warping is spare；Or 1000 kilograms of sterilizing solid mediums are spread
In the shallow pond of indoor cement of prior sterilization, 1-15 meters long, 1-8 meters wide, 5-25 centimetres of culture medium thickness, keeps interior 20-
30 degree, solid medium water content 40-60%, it with the moisturizing of plastic covering film thermal insulating and can prevent from polluting above, be beaten in film
Hole, every square metre 5-15, aperture 2-8 millimeters, stirred every 4 days and mixes once, continuously ferment 8-14 days, terminate, receive halfway
Collect spare；
3. a kind of according to claim 1 have ecological method that is disease-resistant and reducing the place greenhouse gas emissions such as farm concurrently,
It is characterized in that in the biological method, as needed, by concentrating or diluting a in the claims 1, b zymotic fluids
In or solid fermentation matrix in spore quantity control living at every milliliter/or 0.5 hundred million -10 hundred million every gram.
4. a kind of according to claim 1 have ecological method that is disease-resistant and reducing the place greenhouse gas emissions such as farm concurrently,
It is characterized in that in the biological method, as needed with the concrete condition of fermentation, will ferment respectively in a and b of above-mentioned requirements 1
After zymotic fluid or solid fermentation matrix mixed by mass/mass than a: b=0.5-1: 0.5-1, thoroughly mix, i.e.,
Into product.
5. a kind of according to claim 1 have ecological method that is disease-resistant and reducing the place greenhouse gas emissions such as farm concurrently,
It is characterized in that the biological method, when being applied to feces of livestock and poultry and/or organic fertilizer production when place, feces of livestock and poultry is according to new
Fresh 1000 kilograms of excrement applies 1-8 kilograms of mix bacterium agent of the said goods, is uniformly mixed；Household refuse landfill sites press house refuse
1000 kilograms use 0.5-5 kilograms of mix bacterium agent, are uniformly mixed；It is serious and soil-borne disease is tight for farmland especially continuous cropping obstacle
The soil of weight, per acre using above-mentioned 2-4 kilograms of c products, uniformly sprays in agricultural land soil surface, ploughs soil, Huo Zhetong immediately
Shi Shiliang is irrigated, long-term cropping；It can also be used for the ground such as riverine wetland, grassland, forest, it is possible to reduce soil and/or feces of livestock and poultry
Isothermal chamber gas CH4、CO2And N2The formation and discharge of O.
A kind of 6. any ecology side for having the place greenhouse gas emissions such as disease-resistant and reduction farm concurrently of Claims 1 to 5
Application of the method on feces of livestock and poultry, refuse landfill, organic fertilizer production, farmland, forest etc..
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