CN107936113A - 一种鲻鱼鱼鳞铁螯合肽及其制备方法 - Google Patents
一种鲻鱼鱼鳞铁螯合肽及其制备方法 Download PDFInfo
- Publication number
- CN107936113A CN107936113A CN201711316614.0A CN201711316614A CN107936113A CN 107936113 A CN107936113 A CN 107936113A CN 201711316614 A CN201711316614 A CN 201711316614A CN 107936113 A CN107936113 A CN 107936113A
- Authority
- CN
- China
- Prior art keywords
- fish scale
- grey mullet
- mullet fish
- iron chelating
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 title claims abstract description 132
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 78
- 241001593519 Liza affinis Species 0.000 title claims abstract description 73
- 229910052742 iron Inorganic materials 0.000 title claims abstract description 69
- 238000002360 preparation method Methods 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 20
- 230000000694 effects Effects 0.000 claims abstract description 18
- 230000014759 maintenance of location Effects 0.000 claims abstract description 13
- 102000004190 Enzymes Human genes 0.000 claims abstract description 12
- 108090000790 Enzymes Proteins 0.000 claims abstract description 12
- 238000001514 detection method Methods 0.000 claims abstract description 5
- 102000008186 Collagen Human genes 0.000 claims description 49
- 108010035532 Collagen Proteins 0.000 claims description 49
- 229920001436 collagen Polymers 0.000 claims description 49
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 30
- 238000004007 reversed phase HPLC Methods 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 24
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 18
- 241000251468 Actinopterygii Species 0.000 claims description 18
- 239000012153 distilled water Substances 0.000 claims description 18
- 239000000047 product Substances 0.000 claims description 16
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 15
- 238000010828 elution Methods 0.000 claims description 15
- 238000000108 ultra-filtration Methods 0.000 claims description 14
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 12
- 229940088598 enzyme Drugs 0.000 claims description 10
- 238000001641 gel filtration chromatography Methods 0.000 claims description 10
- 238000005227 gel permeation chromatography Methods 0.000 claims description 10
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 8
- 108091005804 Peptidases Proteins 0.000 claims description 7
- 239000004365 Protease Substances 0.000 claims description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 7
- 239000003513 alkali Substances 0.000 claims description 7
- XELZGAJCZANUQH-UHFFFAOYSA-N methyl 1-acetylthieno[3,2-c]pyrazole-5-carboxylate Chemical compound CC(=O)N1N=CC2=C1C=C(C(=O)OC)S2 XELZGAJCZANUQH-UHFFFAOYSA-N 0.000 claims description 7
- 238000000926 separation method Methods 0.000 claims description 7
- 238000002835 absorbance Methods 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 6
- 239000012498 ultrapure water Substances 0.000 claims description 6
- 102000057297 Pepsin A Human genes 0.000 claims description 5
- 108090000284 Pepsin A Proteins 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 238000004440 column chromatography Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- 229940111202 pepsin Drugs 0.000 claims description 5
- 238000010926 purge Methods 0.000 claims description 5
- 239000011347 resin Substances 0.000 claims description 5
- 229920005989 resin Polymers 0.000 claims description 5
- 238000000825 ultraviolet detection Methods 0.000 claims description 5
- 238000010792 warming Methods 0.000 claims description 5
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims 3
- 239000012071 phase Substances 0.000 claims 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims 1
- 102000002322 Egg Proteins Human genes 0.000 claims 1
- 108010000912 Egg Proteins Proteins 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 claims 1
- 235000014103 egg white Nutrition 0.000 claims 1
- 210000000969 egg white Anatomy 0.000 claims 1
- 239000007791 liquid phase Substances 0.000 claims 1
- 238000002791 soaking Methods 0.000 claims 1
- 210000002784 stomach Anatomy 0.000 claims 1
- 238000010521 absorption reaction Methods 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000011084 recovery Methods 0.000 abstract description 4
- 235000019658 bitter taste Nutrition 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 53
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 10
- 238000005119 centrifugation Methods 0.000 description 6
- 108010009736 Protein Hydrolysates Proteins 0.000 description 5
- -1 iron ion Chemical class 0.000 description 5
- 239000003531 protein hydrolysate Substances 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 4
- WTDHULULXKLSOZ-UHFFFAOYSA-N Hydroxylamine hydrochloride Chemical compound Cl.ON WTDHULULXKLSOZ-UHFFFAOYSA-N 0.000 description 4
- 239000011575 calcium Substances 0.000 description 4
- 229910052791 calcium Inorganic materials 0.000 description 4
- 238000004108 freeze drying Methods 0.000 description 4
- 235000011167 hydrochloric acid Nutrition 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 210000004379 membrane Anatomy 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000001914 filtration Methods 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- YNPNZTXNASCQKK-UHFFFAOYSA-N Phenanthrene Natural products C1=CC=C2C3=CC=CC=C3C=CC2=C1 YNPNZTXNASCQKK-UHFFFAOYSA-N 0.000 description 2
- SMWDFEZZVXVKRB-UHFFFAOYSA-N Quinoline Chemical compound N1=CC=CC2=CC=CC=C21 SMWDFEZZVXVKRB-UHFFFAOYSA-N 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000013522 chelant Substances 0.000 description 2
- BEYOBVMPDRKTNR-UHFFFAOYSA-N chembl79759 Chemical class C1=CC(O)=CC=C1N=NC1=CC=CC=C1 BEYOBVMPDRKTNR-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 210000004885 white matter Anatomy 0.000 description 2
- 108010016626 Dipeptides Proteins 0.000 description 1
- 235000002918 Fraxinus excelsior Nutrition 0.000 description 1
- KLKYKPXITJBSNI-CIUDSAMLSA-N Gln-Met-Ala Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C)C(O)=O KLKYKPXITJBSNI-CIUDSAMLSA-N 0.000 description 1
- DBJYVKDPGIFXFO-BQBZGAKWSA-N Gly-Met-Ala Chemical compound [H]NCC(=O)N[C@@H](CCSC)C(=O)N[C@@H](C)C(O)=O DBJYVKDPGIFXFO-BQBZGAKWSA-N 0.000 description 1
- OOCFXNOVSLSHAB-IUCAKERBSA-N Gly-Pro-Pro Chemical compound NCC(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)CCC1 OOCFXNOVSLSHAB-IUCAKERBSA-N 0.000 description 1
- 102000008133 Iron-Binding Proteins Human genes 0.000 description 1
- 108010035210 Iron-Binding Proteins Proteins 0.000 description 1
- 241000212850 Mugil cephalus Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 108010044940 alanylglutamine Proteins 0.000 description 1
- 108010047495 alanylglycine Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000002956 ash Substances 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000004833 fish glue Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000007180 physiological regulation Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 108010087846 prolyl-prolyl-glycine Proteins 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711316614.0A CN107936113B (zh) | 2017-12-12 | 2017-12-12 | 一种鲻鱼鱼鳞铁螯合肽及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711316614.0A CN107936113B (zh) | 2017-12-12 | 2017-12-12 | 一种鲻鱼鱼鳞铁螯合肽及其制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107936113A true CN107936113A (zh) | 2018-04-20 |
CN107936113B CN107936113B (zh) | 2020-11-10 |
Family
ID=61943882
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201711316614.0A Active CN107936113B (zh) | 2017-12-12 | 2017-12-12 | 一种鲻鱼鱼鳞铁螯合肽及其制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107936113B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112471448A (zh) * | 2020-11-19 | 2021-03-12 | 郑州轻工业大学 | 鱼鳞胶原蛋白肽铁锌螯合物的制备方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103788193A (zh) * | 2014-03-06 | 2014-05-14 | 福州大学 | 一种利用双酶协同水解制备金属螯合肽的方法 |
CN104710511A (zh) * | 2015-03-18 | 2015-06-17 | 浙江海洋学院 | 一种源于带鱼鱼肉蛋白的铁螯合肽及其制备方法和用途 |
CN104774896A (zh) * | 2015-04-15 | 2015-07-15 | 浙江海洋学院 | 带鱼鱼骨铁螯合胶原肽制备方法 |
CN105648004A (zh) * | 2015-12-31 | 2016-06-08 | 浙江海洋学院 | 赤魟软骨抗氧化胶原肽的制备方法 |
-
2017
- 2017-12-12 CN CN201711316614.0A patent/CN107936113B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103788193A (zh) * | 2014-03-06 | 2014-05-14 | 福州大学 | 一种利用双酶协同水解制备金属螯合肽的方法 |
CN104710511A (zh) * | 2015-03-18 | 2015-06-17 | 浙江海洋学院 | 一种源于带鱼鱼肉蛋白的铁螯合肽及其制备方法和用途 |
CN104774896A (zh) * | 2015-04-15 | 2015-07-15 | 浙江海洋学院 | 带鱼鱼骨铁螯合胶原肽制备方法 |
CN105648004A (zh) * | 2015-12-31 | 2016-06-08 | 浙江海洋学院 | 赤魟软骨抗氧化胶原肽的制备方法 |
Non-Patent Citations (1)
Title |
---|
林慧敏 等: "超滤法制备高抗菌抗氧化活性带鱼蛋白亚铁螯合肽的工艺研究", 《中国食品学报》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112471448A (zh) * | 2020-11-19 | 2021-03-12 | 郑州轻工业大学 | 鱼鳞胶原蛋白肽铁锌螯合物的制备方法 |
Also Published As
Publication number | Publication date |
---|---|
CN107936113B (zh) | 2020-11-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104710511B (zh) | 一种源于带鱼鱼肉蛋白的铁螯合肽及其制备方法和用途 | |
CN104356200B (zh) | 一种抗氧化肽及其制备方法 | |
CN113151387B (zh) | 一种具有抗氧化和增强免疫功能的鳕鱼皮胶原蛋白肽及其制备方法 | |
KR100904631B1 (ko) | 트랜스 글루타민나제를 이용한 기능성 굴 효소 가수분해물및 그의 제조방법 | |
CN104356201B (zh) | 一种海参抗氧化多肽 | |
CN109265536B (zh) | 一种钙螯合肽及其制备方法和应用 | |
CN103052717A (zh) | 一种工业化生产玉米降压活性肽的方法 | |
CN104402972B (zh) | 一种海参抗氧化肽及其制备方法 | |
CN104774896A (zh) | 带鱼鱼骨铁螯合胶原肽制备方法 | |
CN101589761A (zh) | 一种工业用***籽抗氧化肽的制备方法及应用 | |
CN114230635B (zh) | 一种高活性富硒牡蛎抗氧化肽及其应用 | |
CN109320588A (zh) | 一种刺参来源的ace抑制活性肽 | |
CN107164440A (zh) | 高f值寡肽粗液分离纯化方法 | |
CN108178782A (zh) | 一种鲻鱼鱼鳞铁螯合肽的用途 | |
CN107602665A (zh) | 一种鮸鱼鱼肉抗氧化肽 | |
CN105648008A (zh) | 一种饲用蚕丝抗菌肽制剂及其制备方法 | |
CN104805164A (zh) | 一种具有低致敏性的高蛋白牡蛎活性肽制备方法 | |
CN116162677A (zh) | 一种高纯度鱼皮胶原三肽的制备方法 | |
CN106560518B (zh) | 一种绿侧花海葵抗***癌寡肽的制备方法 | |
CN107936113A (zh) | 一种鲻鱼鱼鳞铁螯合肽及其制备方法 | |
CN104774255B (zh) | 海蜇来源的生物活性肽及其制备方法 | |
CN107759685A (zh) | 一种鲟鱼鱼骨明胶铁螯合肽及其制备方法 | |
CN109680027A (zh) | 一种灰树花小肽铁螯合物及其制备方法和应用 | |
CN104725474A (zh) | 一种金枪鱼肝脏蛋白源钙螯合肽及其制备方法和用途 | |
CN107022593A (zh) | 一种富含脯氨酸多肽的初乳肽及其制备方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240218 Address after: 510000 No. 106, Fengze East Road, Nansha District, Guangzhou City, Guangdong Province (self compiled Building 1) x1301-d013282 (cluster registration) (JM) Patentee after: Guangzhou HuaSu Information Technology Co.,Ltd. Country or region after: China Address before: 316000 No. 1, Haida South Road, Changzhi Island, Lincheng street, Dinghai District, Zhoushan, Zhejiang. Patentee before: Zhejiang Ocean University Country or region before: China |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240226 Address after: No. 051, Fengdeng Street, Qiu County Economic Development Zone, Handan City, Hebei Province, 056000 Patentee after: Zhongke Kangsheng (Hebei) Biotechnology Co.,Ltd. Country or region after: China Address before: 510000 No. 106, Fengze East Road, Nansha District, Guangzhou City, Guangdong Province (self compiled Building 1) x1301-d013282 (cluster registration) (JM) Patentee before: Guangzhou HuaSu Information Technology Co.,Ltd. Country or region before: China |
|
TR01 | Transfer of patent right |