CN107916229A - One plant of Inonotus obliquus and its application - Google Patents
One plant of Inonotus obliquus and its application Download PDFInfo
- Publication number
- CN107916229A CN107916229A CN201710990292.1A CN201710990292A CN107916229A CN 107916229 A CN107916229 A CN 107916229A CN 201710990292 A CN201710990292 A CN 201710990292A CN 107916229 A CN107916229 A CN 107916229A
- Authority
- CN
- China
- Prior art keywords
- inonotus obliquus
- tunning
- culture
- fermentation
- poultry
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H15/00—Fungi; Lichens
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Birds (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Fodder In General (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention provides one plant of Inonotus obliquus（Inonotus obliquus）, China General Microbiological culture presevation administrative center is preserved in, deposit number is CGMCC No. 14549.Present invention also offers a kind of method that above-mentioned bacterial strains submerged fermentation obtains tunning：Bacterial strain is cultivated by parent species, Shaking culture, and seed culture obtains seed liquor, and seed liquor is carried out submerged fermentation obtains zymotic fluid, and zymotic fluid is obtained tunning by precipitation, concentration step.Present invention also offers application of the above-mentioned Inonotus obliquus tunning as poultry feeding additive.The Inonotus obliquus bacterial strain that present invention screening obtains, heritability is stable, the speed of growth is fast, and yield is high, suitable for submerged fermentation.The tunning obtained by the strain makes an addition in poultry diet the production performance that can significantly increase body, reduces production cost；Effectively reduce the use for even substituting the additives such as hormone.
Description
Technical field
The invention belongs to bioengineering and biological technical field, and in particular to the fermentation technique and its hair of fungi Inonotus obliquus
Application of the ferment product in performance of poultry is strengthened.
Background technology
As the improvement of people's living standards, being increasingly enhanced for the quality requirement of livestock products, food security is asked in recent years
Constantly showing for topic, even more allows attention of the whole society to animal food safety to reach unprecedented height.How
Birds epidemic disease continues use, reduction birds drug resistance and the secondary work of other poison that reduction under background occurred frequently even prevents antibiotic
With realizing green and healthy cultivation and the food security of birds, find safe efficient, green feed additive and immune increase
Strong agent alternatives to medication becomes the most important thing of aquaculture development.Medicinal fungi becomes novel fodder additive with the effect of its uniqueness
With the potential goal in research of immunopotentiator, correlative study has shown that fungal extract or preparation can effectively facilitate the life of animal
Production and immune performance.
Inonotus obliquus（Inonotus obliquus）As one kind of medicinal fungi, rich in polysaccharide, triterpene, sterol, total phenol
Isoreactivity component, is applied to disease, the recent research such as stomach cancer, intestinal cancer, heart disease, diabetes by the mankind already and shows its fructification
Also have in human anti-tumor, anti-oxidant, immunological regulation, hypoglycemic, antiviral, antibacterium, anti parasitic etc. with extract
The effect of unique.But Inonotus obliquus kind is existing less, fructification is also seldom formed, therefore wild resource is extremely limited.Carry out true
The strain breeding thereof and artificial culture of bacterium are the important means for obtaining high-quality Inonotus obliquus, but through long felt, could not be obtained all the time
Breakthrough.The progress of fermentation engineering makes it possible the cracking of resource problem.The fermentation of fungi generally uses deep layer
Fermentation technique, it be aeration process technology it is improved on the basis of microbial cell be placed in Liquid Substrate cultivated,
A large amount of mycelium and other metabolites are obtained with this.It is adapted to the fungi of submerged fermentation should have the characteristics that：（1）Mycelium egg
Culture under the conditions of white matter rich content and adaptation liquid ventilation；（2）The speed of growth is fast and high yield；（3）With stable heredity
Characteristic and identifiable morphological feature.Meanwhile adaptability presence of the different strains of fungi of the same race to Submerged fermentation is poor
It is different, thus to carry out the deep layer research of fungi and product development first have to solve the problems, such as be exactly Suitable strains screening.
Can Inonotus obliquus effect be notable, but screen suitable bacterial strain and its tunning and improve the production of birds
Performance, the exploitation for novel fodder additive are unclear.
The content of the invention
It is suitable for the bacterial strain rareness of submerged fermentation for Inonotus obliquus and poultry cultivation lacks safe efficient, the new feeding of green
The problems such as feed additives, it is an object of the present invention to provide the Inonotus obliquus of one plant of suitable submerged fermentation；The mesh of the present invention
Two be preparation method that above-mentioned Inonotus obliquus tunning is provided；The third object of the present invention is to provide a kind of birch brown
Application of the pore fungi tunning in performance of poultry is strengthened.
To achieve the above object, the present invention adopts the following technical scheme that.
One plant of Inonotus obliquus（Inonotus obliquus）YU, is preserved in China General Microbiological culture presevation management
The heart, deposit number are CGMCC No. 14549；Its ITS sequence is as shown in SEQ No. 1.
A kind of method for obtaining above-mentioned Inonotus obliquus tunning, using following steps：
（1）Parent species are cultivated：Inonotus obliquus is inoculated in culture on test tube slant and obtains parent species；
（2）Shaking culture：Parent species are inoculated in fluid nutrient medium culture, obtain inoculation liquid；
（3）Seed culture：Inoculation liquid is inoculated in the fluid nutrient medium in seeding tank, air agitation fermentation obtains seed liquor；
（4）Submerged fermentation：Seed liquor is inoculated in the fluid nutrient medium of fermentation tank, air agitation fermentation；
（5）Precipitation：Zymotic fluid is transferred to settling tank, flocculant is added, is filtered after precipitation, obtain supernatant；
（6）Concentration：Supernatant is transferred to vacuum concentration pot, certain density discharging is concentrated into, up to tunning.
The fluid nutrient medium includes following components（g/L）：Starch 10, peptone 10, glucose 15, potassium dihydrogen phosphate 1,
Magnesium sulfate 0.5, vitamin B10.1, soya-bean oil 0.2；Alternatively, the pH of fluid nutrient medium is 6.8-7.4.
Alternatively, step（1）In, inclined-plane is potato dextrose agar（PDA）, cultivation temperature is 25-28 DEG C,
Incubation time is 5-7 days.
Alternatively, step（2）In, it is inoculated with the mycelium or spore suspension for parent species；Preferably mycelium.
Alternatively, cultivation temperature is 25-28 DEG C；Incubation time is 3-10 days；Shaking speed is 150-200 revs/min.
Alternatively, step（2）In, parent species obtain inoculation liquid through multistage Shaking culture；Two level and above Shaking culture connect
Kind amount is 8-15%；Preferably, parent species obtain inoculation liquid through two level culture.
Alternatively, step（3）And step（4）In, inoculum concentration 8-15%；Total liquid volume is fermentation tankage size in tank
50-80%；Cultivation temperature is 25-28 DEG C；Incubation time is 7-15 days；Speed of agitator is 100-200 revs/min；Throughput is
0.1-0.5vvm。
Alternatively, step（3）In, inoculation liquid obtains seed liquor through multistage seed culture；Preferably, inoculation liquid is trained through two level
Support and obtain seed liquor.
Step（5）Described in flocculant be chitosan, preferable additive amount is the 0.05-0.2% of zymotic fluid weight.
Preferably, step（5）The biomass of middle zymotic fluid is more than 20g/L.
As an optimization, a period of time is heated and kept before precipitation；More optimally, heating-up temperature is 70-95 DEG C；During insulation
Between be 4-6h.
Alternatively, step（6）It is 1.035-1.065 to be concentrated into relative density.
Preferably, step（6）The step of concentrate is dried to solid is further included afterwards.
A kind of application of Inonotus obliquus tunning prepared by above method as poultry feeding additive.
Alternatively, additive amount of the Inonotus obliquus tunning in poultry feed is 0.2-2wt%；Most preferably 0.8wt%.
Alternatively, Inonotus obliquus tunning is 0.1-5wt% in the additive amount that poultry drinks water, and is preferably 0.5-2.0
wt%。
Beneficial effects of the present invention：The Inonotus obliquus YU bacterial strains that present invention screening obtains, heritability is stable, the speed of growth
It hurry up, yield is high, can be as the strain of submerged fermentation suitable for submerged fermentation.The tunning obtained by the strain makes an addition to poultry
The production performance of body can be significantly increased in daily ration, effectively lifts the average daily gain of poultry, reduces food consumption rate and material
Meat ratio.Tunning provided by the invention can realize the efficient cultivation of poultry, reduce production cost；Effectively reduce and even substitute
The use of the additives such as hormone, antibiotic, ensures food safety, the strong sound development for promoting industry.
Brief description of the drawings
Fig. 1 feeds for Inonotus obliquus tunning influences chicken daily gain；
Fig. 2 feeds for Inonotus obliquus tunning influences chicken feedstuff-meat ratio；
Fig. 3 feeds for astragalus polyose with Inonotus obliquus tunning influences chicken daily gain；
Fig. 4 feeds for astragalus polyose with Inonotus obliquus tunning influences chicken feedstuff-meat ratio.
Fungi preservation information
Inonotus obliquus（Inonotus obliquus）China General Microbiological culture presevation management is stored within 5th in September in 2017
Center, preservation address are Institute of Microorganism, Academia Sinica of Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 BeiJing, China, are protected
It is CGMCC No. 14549 to deposit numbering.
Embodiment
With reference to embodiment and attached drawing, the present invention will be further described, but the present invention is from the limit of following embodiments
System.
The separation identification of 1 Inonotus obliquus of embodiment.
JX plants of Inonotus obliquus has been identified as Inonotus obliquus quoted from Biology Inst., Hebei Academy of Sciences.YU plants of Inonotus obliquus
For wild strain, gather from Dali City, yunnan Province.The fructification of no disease and pests harm is removed from surface of trees during collection, sampling depth
For 1-3 centimetres of trees top layer, it is fitted into Riker mount and returns to the isolation and purification that laboratory carries out next step.Separation strain uses umbrella
Bacterium partition method, first removes fructification surface sawdust and impurity with distilled water, then carries out surface sterilization with 75% ethanol, is transferred to ultra-clean
Break fructification after platform into two with one's hands, and it is sterile take deep tissue, be put into the red solid medium of tiger rapidly, be placed in constant incubator（28℃）
Middle culture 3 days, after the bacterium of purifying is transferred in PDA solid mediums, it is daily to observe colony growth situation.Then to improve CTAB
The Inonotus obliquus genome of method extraction is template, ITS-rDNA universal primers ITS1：5 ' TCCGTAGGTGAACCTGCGG3 ' and
ITS2：5 ' TCCTCCGCTTATTGATATGC3 ' carry out PCR amplification for specific primer, carry out the identification of bacterial strain.Amplification obtains
ITS sequence as shown in SEQ No. 1, itself and the Inonotus obliquus in GeneBank（DQ103883）ITS-rDNA sequences have
More than 99% homology, judges separated wild mushroom for Inonotus obliquus, and YU plants entitled, which protected on the 5th in September in 2017
China General Microbiological culture presevation administrative center is stored in, it is CGMCC No. 14549 to preserve numbering.
The acquisition of 2 Inonotus obliquus tunning of embodiment.
Prepare fluid nutrient medium：According to fluid nutrient medium dosage, following component is weighed respectively（g/L）：Peptone 10, grape
Sugar 15, potassium dihydrogen phosphate 1, magnesium sulfate 0.5, vitamin B10.1st, soya-bean oil 0.2, aqua sterilisa are mended to required volume, and adjustment pH is extremely
7.0,121 DEG C of 30min that sterilize, it is spare.
（1）Parent species are cultivated：The strain that Inonotus obliquus in embodiment 1 preserves is inoculated in test tube slant potato glucose fine jade
Fat culture medium（PDA）In, transfer 2 times, activated 7 days under the conditions of 25 DEG C every time, be parent species, it is spare；
（2）Shaking culture：Female bacterium is turned in 500mL level-one nutrient solutions, cultivates 10 days, is then transferred to two level nutrient solution, is continued
Culture 5 days, 28 DEG C of shaking table temperature, 180 revs/min of rotating speed, culture end take a small amount of culture to carry out sediments microscope inspection, no miscellaneous bacteria into
Enter lower step；
（3）Seed culture：Liquid strain bacterium is inoculated in 7L fluid nutrient mediums by 10% amount and is fitted into 10L fermentation tanks, 25 DEG C logical
Air culture is supported 10 days, then is transferred in the 100L fermentation tanks equipped with 70L fluid nutrient mediums, and 25 DEG C of ventilations are mixed 10 days；
（4）Submerged fermentation：Fermentate is transferred in the 1000kg fermentation tanks equipped with 700kg fluid nutrient mediums, 25 DEG C of ventilation mixing
Culture 10 days；
（5）Precipitation：When biomass reaches 20g/L in detection fermentation tank, stop fermentation, fermentation tank is warming up to 90 degree, insulation 4 is small
When, then zymotic fluid is transferred to settling tank, the chitosan of 0.1% zymotic fluid weight is added, when precipitation 48 is small, with half frame filter mistake
Filter supernatant；
（6）Concentration：Supernatant is transferred to vacuum concentration pot to be concentrated, measures when relative density is 1.035 and completes tunning
Preparation, packing.
3 birch fuscoporia ferrugineo-fusca teng submerged fermentation performance of embodiment.
Inonotus obliquus YU and JX strain in embodiment 1 are pressed into step in embodiment 2（1）-（4）Submerged fermentation is carried out, respectively
Each 100mL of zymotic fluid is taken, is centrifuged, precipitation is collected, is centrifuged again after being washed with deionized water, precipitation is collected after being repeated 3 times, 105
Drying is weighed under the conditions of DEG C, measures biomass.The results are shown in Table 1.By 1 data of table, YU plants of fermentation indexs of bacterial strain are most
Excellent, hypha form is excellent, and growth potential is strong, and biomass increases obvious；And JX plants of nearly no growths, it is not suitable for submerged fermentation.
1 different strains Liquid Culture result of table
| Bacterial strain | Whether there is miscellaneous bacteria | Hypha form | Biomass（g/L） |
| YU | Nothing | Mycelia is thick, and inside is enriched | 28.43 |
| JX | Nothing | Mycelia is few, more carefully | 2.15 |
Enhancing of the 4 Inonotus obliquus tunning of embodiment to performance of poultry.
Influence of the 4.1 Different adding amount Inonotus obliquus tunnings to chicken daily gain and feedstuff-meat ratio
40 1 age in days SPF chickens are divided into I, II, III, IV, V group, wherein I-IV groups are added in embodiment 2 in feed respectively
Inonotus obliquus tunning, additive amount 0.2%, 0.4%, 0.8%, 2%, remaining one group is control group, and it is brown not add birch in daily ration
Pore fungi tunning.Raise respectively in isolator, feeding period 42 days, during which free choice feeding.Respectively at the 7th, 14,21,28,
35th, weigh to chicken within 42 days, go on a hunger strike 12h before weighing.The forage volume of weightening and the consumption of chicken body is calculated, and then obtains difference
Average daily gain, average daily gain and the feedstuff-meat ratio in period, its calculation are：
Average daily gain（ADG）=（End of term weight-phase initial weight）/ feeding number of days
Average daily gain（ADFI）=period total feed consumption amount/feeding number of days
Feedstuff-meat ratio（F/G）=average daily gain/average daily gain.
Using minute as abscissa, Fig. 1 is made for ordinate with weightening；Using minute as abscissa, using feedstuff-meat ratio as
Ordinate makees Fig. 2.The results show that in average weight gain value index, compared to control group, before 21 days, difference addition concentration
Weightening of the Inonotus obliquus fermentate to chicken has facilitation effect；After 21 days, in addition to II group, other group of gaining effect is still higher than
Control group.In terms of feedstuff-meat ratio index, the feedstuff-meat ratio of each group is below control group, shows that Inonotus obliquus additive can effectively improve
The rate of returns of feed.In conclusion the Inonotus obliquus tunning of the present invention can strengthen the production performance of poultry, 0.8% adds
Add concentration best results.
4.2 Inonotus obliquus tunnings and influence of the astragalus polyose to chicken daily gain and feedstuff-meat ratio
Astragalus polyose is that current research is more goed deep into, using relatively broad important polysaccharide immunopotentiator.Research shows,
The astragalus polyose of suitable concentration is added in feed can strengthen the production performance and immune performance of poultry.In order to more intuitively embody
Effect of the Inonotus obliquus in terms of performance of poultry is strengthened, using astragalus polyose as reference, carries out both comparative studies.
If 2 groups of test group（I and II）, 1 group of control group（III）, every group of 1 age in days SPF chicken 20.I group is added in daily ration
The Inonotus obliquus tunning of 0.8% concentration, II group is added astragalus polyose in daily ration（Have purchased from Beijing life Tetracoq skill share
Limit company, it is proposed that feeding additive amount 0.2%）.Test method is as described in 4.2, using minute as abscissa, is sat with weightening to be vertical
It is denoted as Fig. 3；Using minute as abscissa, make Fig. 4 by ordinate of feedstuff-meat ratio.The results show that two kinds of additives can promote
Into the increase of each experimental period average weight and the decline of feed consumption rate and feedstuff-meat ratio, but it is compared to each other, the effect of Inonotus obliquus is more
Significantly：Within 42 day experimental period, Inonotus obliquus fermentate group weight gain is averagely higher by astragalus polyose group 9.67mg, feedstuff-meat ratio
It is average then reduce by 0.156.
<110>Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricul
Qinhuangdao high pass bio tech ltd
<120>One plant of Inonotus obliquus and its application
<130> 20170915
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 798
<212> DNA
<213> Inonotus obliquus
<400> 1
tccgtaggtg aacctgcgga aggatcatta tcgagtttat tttgaaatcg aggggcctgt 60
gctggcacgg aaacgtttgc atgtgcacgg cctttcgtgc tcaaatccaa ctctcaaacc 120
cctgtgcacc tatacaagtt gaaggtctta gtagtttctg taatcgaacg gcaagtcaag 180
tacgtcgagt aatcaagtac gagggtttcg gcccttggaa agtgtgaaag atggaaaggc 240
aagcttcaga gacaaggaga cgaaaagctt ttggcttcat tacaaacacc aatataattg 300
ttatgtgaat gaaatgctcc ttgtgggcga taataaatac aactttcaac aacggatctc 360
taggctctcg catcgatgaa gaacgcagcg aaatgcgata agtaatgtga attgcagaat 420
tcagtgaatc atcgaatctt tgaacgcacc ttgcgcccct tggtattccg aggggcatgc 480
ctgtttgagt gtcatgttaa tctcaaatcg ctcgtctatt cttaattgaa gtggctttcg 540
atttggactt ggaggttttg ctggcccggg cgactttggt tgcccttggt ttgtcggctc 600
ctctcaaata cattagctgg actttggttc gcgtttacgg tgtaataatg taatgttcac 660
taagacgctt gcctaacaag tctgcttcta atagtcctta agttggacaa ggatcccttc 720
gttgggcctt cttgacacct ttgacctcaa atcaggtagg attacccgct gaacttaagc 780
atatcaataa gcggagga 798
Claims (10)
1. one plant of Inonotus obliquus（Inonotus obliquus）, China General Microbiological culture presevation administrative center is preserved in, is protected
It is CGMCC No. 14549 to hide numbering.
A kind of 2. method for the tunning for obtaining Inonotus obliquus as claimed in claim 1, it is characterised in that use following step
Suddenly：
（1）Parent species are cultivated：Inonotus obliquus is inoculated in culture on test tube slant and obtains parent species；
（2）Shaking culture：Parent species are inoculated in fluid nutrient medium culture, obtain inoculation liquid；
（3）Seed culture：Inoculation liquid is inoculated in the fluid nutrient medium in seeding tank, air agitation fermentation obtains seed liquor；
（4）Submerged fermentation：Seed liquor is inoculated in the fluid nutrient medium of fermentation tank, air agitation fermentation；
（5）Precipitation：Zymotic fluid is transferred to settling tank, flocculant is added, is filtered after precipitation, obtain supernatant；
（6）Concentration：Supernatant is transferred to vacuum concentration pot, certain density discharging is concentrated into, up to tunning.
3. preparation method according to claim 2, it is characterised in that step（3）And step（4）In, inoculum concentration 8-
15%；Throughput is 0.1-0.5vvm.
4. preparation method according to claim 2, it is characterised in that step（5）The biomass of middle zymotic fluid for 20g/L with
On.
5. according to the method described in claim 2, it is characterized in that, step（5）Zymotic fluid heating process is further included before middle precipitation,
Heating-up temperature is 70-95 DEG C；Soaking time is 4-6h.
6. according to the method described in claim 2, it is characterized in that, step（5）Middle flocculant is chitosan.
7. according to the method described in claim 2, it is characterized in that, step（6）Further include afterwards and concentrate is dried to solid
The step of.
A kind of 8. answering as poultry feeding additive such as Inonotus obliquus tunning prepared by claim 2-7 either method
With.
9. application according to claim 8, it is characterised in that additive amount of the Inonotus obliquus tunning in poultry feed
For 0.2-2wt%.
10. application according to claim 8, it is characterised in that the additive amount that Inonotus obliquus tunning is drunk water in poultry
For 0.1-5wt%.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710990292.1A CN107916229B (en) | 2017-10-23 | 2017-10-23 | One plant of Inonotus obliquus and its application |
| KR1020187029131A KR102195870B1 (en) | 2017-10-23 | 2018-04-03 | Chaga fungus and its application |
| PCT/CN2018/081658 WO2019080454A1 (en) | 2017-10-23 | 2018-04-03 | Inonotus obliquus and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710990292.1A CN107916229B (en) | 2017-10-23 | 2017-10-23 | One plant of Inonotus obliquus and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107916229A true CN107916229A (en) | 2018-04-17 |
| CN107916229B CN107916229B (en) | 2019-11-08 |
Family
ID=61894891
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710990292.1A Active CN107916229B (en) | 2017-10-23 | 2017-10-23 | One plant of Inonotus obliquus and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107916229B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110117548A (en) * | 2019-05-27 | 2019-08-13 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of thin skin fibre pore fungi new strains and its artificial cultivation method and purposes |
| CN114456943A (en) * | 2020-11-09 | 2022-05-10 | 浙江养生堂天然药物研究所有限公司 | Inonotus obliquus and extract and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1556194A (en) * | 2004-01-07 | 2004-12-22 | 敖宗华 | Technology for producing birch brown fungus extract and saccharide using large scale liquid submerged fermentation process |
| CN101933460A (en) * | 2009-12-22 | 2011-01-05 | 辽宁大学 | Inonotus obliquus and method for extracting triterpennoids from same |
| KR20120013521A (en) * | 2010-08-05 | 2012-02-15 | 봉 환 정 | Method for producing fermented solution of mushroom with enhanced antioxidative activity and fermented solution of mushroom produced by the same |
| CN103156052A (en) * | 2013-02-21 | 2013-06-19 | 徐向群 | Submerged fermentation technology of producing immunological enhancement active materials of inonotus obliquus |
| CN103283990A (en) * | 2013-05-07 | 2013-09-11 | 徐向群 | Application of inonotus obliquus polysaccharide used as feed additive for litopenaeus vannamei |
-
2017
- 2017-10-23 CN CN201710990292.1A patent/CN107916229B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1556194A (en) * | 2004-01-07 | 2004-12-22 | 敖宗华 | Technology for producing birch brown fungus extract and saccharide using large scale liquid submerged fermentation process |
| CN101933460A (en) * | 2009-12-22 | 2011-01-05 | 辽宁大学 | Inonotus obliquus and method for extracting triterpennoids from same |
| KR20120013521A (en) * | 2010-08-05 | 2012-02-15 | 봉 환 정 | Method for producing fermented solution of mushroom with enhanced antioxidative activity and fermented solution of mushroom produced by the same |
| CN103156052A (en) * | 2013-02-21 | 2013-06-19 | 徐向群 | Submerged fermentation technology of producing immunological enhancement active materials of inonotus obliquus |
| CN103283990A (en) * | 2013-05-07 | 2013-09-11 | 徐向群 | Application of inonotus obliquus polysaccharide used as feed additive for litopenaeus vannamei |
Non-Patent Citations (1)
| Title |
|---|
| 武永德等: "两种不同来源桦褐孔菌多糖免疫活性的比较", 《浙江理工大学学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110117548A (en) * | 2019-05-27 | 2019-08-13 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of thin skin fibre pore fungi new strains and its artificial cultivation method and purposes |
| CN114456943A (en) * | 2020-11-09 | 2022-05-10 | 浙江养生堂天然药物研究所有限公司 | Inonotus obliquus and extract and application thereof |
| CN114456943B (en) * | 2020-11-09 | 2023-12-22 | 浙江养生堂天然药物研究所有限公司 | Inonotus obliquus and extract and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107916229B (en) | 2019-11-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102195870B1 (en) | Chaga fungus and its application | |
| CN107245457A (en) | A kind of extracting method and application of dendrobium candidum endogenetic fungal bacterial strain and its exocellular polysaccharide of generation and the exocellular polysaccharide | |
| CN108865953A (en) | One plant of wide spectrum inhibits bacillus and its composite bacteria preparation of aquatic products vibrio pathogen | |
| CN101829159B (en) | Method for preparing selenium-enriched monkey head mushroom capsule | |
| CN103981102B (en) | DSE bacterial strain 24L-4 and the application on Herba Dendrobii is produced thereof | |
| CN103396956B (en) | A kind of saccharomyces cerevisiae and its screening and culturing method and the method for bean pulp fermentation | |
| CN106544242B (en) | Preparation method of beautiful cordyceps sobolifera wine | |
| CN117417840B (en) | Exotomycorrhizal fungi Yunnan sclerotium lappa ZSS01 and application thereof | |
| CN106801017A (en) | The cordyceps militaris link bacterial strain screening of a kind of high yield thermophilic protease and cordycepin and method of mutagenesis | |
| CN104585510B (en) | A kind of mixed fungus fermentation thing feed addictive and preparation method thereof | |
| CN102925527A (en) | Method for mixing and fermenting flammulina velutipes and lucid ganoderma | |
| CN107916229B (en) | One plant of Inonotus obliquus and its application | |
| CN110093283B (en) | Beauveria bassiana strain and culture method thereof | |
| CN103535525B (en) | Production method of biological feed additive rich in amino acids and proteins | |
| CN107550946A (en) | A kind of preparation and application of poultry immunity reinforcing agent | |
| CN105695346B (en) | The sclerotium mould and its preparation method and application of one plant of intoxicating plant parasitical eelworm | |
| CN102174414B (en) | Application of new morchella costata M8-13 liquid fermentation substance in development of health-care products and medicaments | |
| CN115992080B (en) | Compound microbial agent and feed for inducing generation of hermetia illucens larva antibacterial peptide as well as application methods and application of compound microbial agent and feed | |
| CN104894026B (en) | The cultural method of bafillus natto and its application | |
| CN107027516A (en) | A kind of selenium-rich Periostracum cicadae, its cultural method and application | |
| CN100412186C (en) | Tinder fungus and process for deep liquid fermentation preparation of tinder fungus | |
| CN106544280B (en) | A kind of resistance meat duck yeast for animal feeds bacterial strain and application | |
| CN104988183A (en) | Preparation method of pleurotus ostreatus fermentation broth and application of fermentation broth in degradation of aflatoxin B1 | |
| CN111363692B (en) | Complex microbial inoculant and application of fermentation product thereof | |
| CN103039739A (en) | Application of entomogenous fungi liquid fermentation mycelium as prawn feed additive |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |