CN107904141A - The reactor of ripple mixing control algae in a kind of simulation - Google Patents
The reactor of ripple mixing control algae in a kind of simulation Download PDFInfo
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- CN107904141A CN107904141A CN201711423742.5A CN201711423742A CN107904141A CN 107904141 A CN107904141 A CN 107904141A CN 201711423742 A CN201711423742 A CN 201711423742A CN 107904141 A CN107904141 A CN 107904141A
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- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C12M31/00—Means for providing, directing, scattering or concentrating light
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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Abstract
The invention discloses a kind of reactor of ripple mixing control algae in simulation, including constant water bath box and it is arranged in constant water bath box and open-topped interior case；Wherein, spiral in interior case circumferential direction and carbon fiber diving heater wire is set, and carbon fiber diving heater wire is connected with the temperature detect switch (TDS) being arranged on outside constant water bath box；The opening of interior case is provided with cover board, and cover board is equipped with light-emitting device and aerator, and the illumination of light-emitting device, towards in interior case, aerator is connected with the uniform aeration head being arranged in interior case；The side wall of interior case has been sequentially communicated inlet tube and outlet tube from top to bottom, and inlet tube and outlet tube are extend out to outside constant water bath box；Light-emitting device on cover board includes the SCM system for being used to adjust some LED light strip intensities of illumination for being located at some LED light strip of cover board bottom and being located at the top of cover board, receive the cyclically-varying of intensity of illumination using algae under wavy state in light-emitting device simulation layering lake storehouse, study suppression growth and the stress adaptation mechanism of algae.
Description
Technical field
The invention belongs to lake and reservoir ocean water body contamination control field, and in particular to ripple mixing control algae in a kind of simulation
Reactor, the periodicity for simulating intensity of illumination suffered by algae under the conditions of interior ripple by periodically-varied intensity of illumination become
Change, and then the optimal conditions that the effect of ripple control algae, mechanism and technology are applied in quantitative study.
Background technology
In recent years, each lake in the whole nation, Reservoir Eutrophication state are serious, and the increase of alga eruption frequency and degree aggravate.
In summer, the rise of lake storehouse water surface temperature, temperature gradient, the wherein larger jump temperature layer of temperature gradient are formed in the vertical section of Hu Ku
Prevent natural vertical circulation in water body, and interior ripple has powerful mixed function as a kind of " deep water blender ", can be with
Cut down temperature gradient, destroy water temperature delamination, dissolved oxygen concentration inside increase water body, suppresses internal contamination in lake bottom storehouse mud and release
Put and strengthen self-purification of waters, hence it is evident that improve water quality.Meanwhile work place study is, it was also found that the interior ripple (such as wind-force) of self-assembling formation
The vertical reciprocal migration of algae inside water body can be caused, so that temperature, intensity of illumination, dissolved oxygen and nutrition at where causing algae
The cyclic fluctuation of the factors such as salinity, wherein intensity of illumination increase with the depth of water and have exponent relation decay, cause intensity of illumination
Influence to algal grown is maximum；But in natural stratified water, since the external environment condition and the factor that influence algal grown are non-
It is often complicated, can not in Quantitative evaluation ripple for the control effect of algae, mechanism in water.In natural stratified water, it can also use
Ripple in artificial induction's generation such as bottom aeration or mechanical cycles, causes the periodicity of algal grown condition (especially intensity of illumination)
Fluctuation, so as to suppress the growth of algae, but also can not quantitatively predict the control algae effect of ripple in artificial induction.If it is desired to interior ripple
Control algae to be used in actual lake storehouse body eutrophication control engineering, be also required to control it algae effect, mechanism and optimal conditions first
Carry out system research；But shortage can simulate the reactor of intensity of illumination cyclic fluctuation under the conditions of interior ripple at present, lead to not
Under the controlled condition of laboratory qualitative, quantitative research in ripple mixing algae control technology principle and optimal conditions.
The content of the invention
The object of the present invention is to provide a kind of reactor of ripple mixing control algae in simulation, for simulating under interior ripple existence condition
Intensity of illumination cyclic fluctuation, and then qualitative, quantitative research is in different illumination intensity period of waves and intensity of illumination amplitude of variation bar
The growth characteristics of algae under part, effect, mechanism and the optimal parameter of ripple control algae in preliminary analysis.
The present invention adopts the following technical scheme that to realize：
The reactor of ripple mixing control algae in a kind of simulation, including constant water bath box and be arranged in constant water bath box and push up
The interior case of portion's opening；Wherein,
The carbon fiber diving heater wire to spiral in interior case circumferential direction, and carbon fiber diving heating are provided with constant water bath box
Line is connected with the temperature detect switch (TDS) being arranged on outside constant water bath box；
The opening of interior case is provided with cover board, and light-emitting device and aerator, and the light of light-emitting device are provided with cover board
According to towards in interior case, aerator is connected with the uniform aeration head being arranged in interior case；The side wall of interior case connects successively from top to bottom
Inlet tube and outlet tube are connected with, inlet tube and outlet tube are extend out to outside constant water bath box.
Further improve of the invention is that cover board is aluminum plate.
Further improve of the invention is that aerator includes being arranged on the aeration pump and control aeration at the top of cover board
The time switch of pump work, the gas outlet of aeration pump are connected with uniform aeration head.
Further improve of the invention is, metal temperature probe is additionally provided with interior case, it is with being arranged on water bath with thermostatic control
Electron temperature control flow connection outside case, electron temperature control flow are connected with temperature detect switch (TDS), and temperature detect switch (TDS) adds with carbon fiber diving
Hot line connects.
Of the invention further improve be, the first protrusion and second convex is diagonally respectively arranged with the inner wall of interior case
Go out, for limiting the installation site of cover board.
Further improve of the invention is that the bottom four corners of interior case are additionally provided with four support columns.
Of the invention further improve be, is provided with the first screw knob and the first valve on inlet tube, on outlet tube
It is provided with the second screw knob and the second valve.
Further improve of the invention is that light-emitting device includes being arranged on some LED light strip of cover board bottom, Yi Jishe
Put the SCM system for being used to adjust some LED light strip intensities of illumination at the top of cover board.
Of the invention further improve be, according to existing for ripple in stratified lake reservoir typical case 5~30m of depth of water scope with
And interior 0.5~24h of wave period, according to intensity of illumination and the exponential decay dependence of the depth of water, calculate illumination under the conditions of the Ku Neibo of lake
Typical strengths and periodic regime, the intensity of illumination of some LED light strip is controlled using SCM system so that intensity of illumination is in meter
Within the Typical strengths and periodic regime of calculation.
The present invention has following beneficial technique effect：
The reactor of ripple mixing control algae, water temperature is precisely controlled using constant water bath box in a kind of simulation provided by the invention,
Control dissolved oxygen concentration suitable by aeration pump and uniform aeration head, control light-emitting device using SCM system, make illumination strong
Degree is in cyclically-varying under different amplitude ranges and periodic condition, and to simulate, algae is hung down under the conditions of interior ripple in actual stratified water
The different fluctuation illumination conditions received during to periodic reverse motion, can study different fluctuation illumination bars in laboratory quantitative simulation
The growth course and adaptation mechanism of algae under part, so study in difference ripple wave amplitude and interior ripple control algae effect under periodic condition and
Mechanism；It is a kind of reactor of ripple mixing control algae in simulation simple in structure, function is polynary, easily controllable.
Further, using aluminum plate cover board, the entrance of external contamination material can be blocked, ensures to exempt from during algal grown
Influenced by other external contaminations；Also can bear the high temperature of LED light strip for a long time, and using aluminium good heat transfer capacity in time with
Heat exchange is carried out outside reactor, maintains the metastable temperature conditionss of inside reactor.
Further, using LED light strip, the intensity of illumination that LED light strip is controlled by programmable SCM system is in rule
Property change, can accurately simulate wave field in difference and close the intensity of illumination cyclic fluctuation bar received during the vertical reciprocating motion of lower algae
Part；Using STC8952RC/LE52RC microcontrollers as kernel control chip, aid in driving with motor drive module, DC power supply, light modulation
Dynamic device, the rule exponentially successively decreased with the depth of water with intensity of illumination under simulation water using the cyclically-varying of LED light strip intensity of illumination
Rule, the similar intensity of illumination that algae receives when creating ripple in occurring with actual layering Hu Ku fluctuate environment.
Further, being supplied using aeration pump, the gas outlet of aeration pump is connected with the aeration head that reactor bottom is evenly arranged,
Uniform aeration is realized by increasing aeration area；And it can ensure algae by opening the frequency and single aeration time of aeration pump
Liquid oxygen gas is sufficient, allows dissolved oxygen to become the nonrestrictive factors of algal grown, main to investigate intensity of illumination fluctuation to algal grown
Influence cross increase aeration area to realize uniform aeration.
Further, water temperature in reactor is monitored using the metal temperature probe being arranged in interior case in real time, by water temperature information
Electron temperature control flow is shown in time, makes temperature intuitively visible；And real-time water temperature information is fed back to and is arranged on electron temperature
Control unit below control flow, according to the constant water temperature being previously set in reactor, is turned on and off temperature detect switch (TDS) to control
The working status of carbon fiber diving heater wire, in real time and accurately controls water temperature in reactor, allows water temperature to become algal grown
Nonrestrictive factors, main intensity of illumination of investigating fluctuates influence to algal grown.
Further, be diagonally respectively arranged with the inner wall of interior case the first protrusion and second protrusion, can be used for limitation and
The installation site of fixed aluminum plate cover board, ensures there are enough heat exchange cushion spaces above algae solution, remains relatively steady in reactor
Fixed temperature environment.
Further, the bottom four corners of interior case are additionally provided with four support columns, and interior case can be made to be fixed on certain position, ensure anti-
Answer device and algal grown conditional stability.
Further, the first screw knob and the first valve are provided with inlet tube, the rotation of the second screw thread is provided with outlet tube
Button and the second valve, rapid liquid inlet and can go out liquid, facilitate the switching that different illumination intensity surging condition is tested to carry out.
Further, the power of the LED light strip of light-emitting device causes algae to connect according to actual be layered in the storehouse of lake in generation during ripple
The typical intensity of illumination received is rationally designed, 5~30m of typical depth of water scope first according to existing for ripple in stratified lake reservoir
And interior 0.5~24h of wave period, according to intensity of illumination and the exponential decay dependence of the depth of water, calculate illumination under the conditions of the Ku Neibo of lake
Typical strengths and periodic regime, the power of some LED light strip is controlled using SCM system, makes the light that LED light strip sends
Intensity of illumination is within the Typical strengths and periodic regime calculated, and the achievement in research for making to obtain using the present invention has very strong
Specific aim and science.
Brief description of the drawings
Fig. 1 is the structure diagram of the reactor of ripple mixing control algae in a kind of simulation of the present invention.
Fig. 2 is SCM system connected mode schematic diagram.
Fig. 3 is the intensity of illumination of cyclic fluctuation under the conditions of the interior ripple simulated using the present invention.
Fig. 4 is influence of the intensity of illumination cyclic fluctuation to algal grown.
In figure：1- temperature detect switch (TDS)s, case in 2-, the first screw knobs of 3-, 4- constant water bath box, 5- electron temperature control flows,
6- metal temperatures are popped one's head in, 7- cover boards, 8-LED light bar, 9- SCM systems, 10- time switches, 11- aeration pumps, the uniform cloth of 12-
Gas head, 13- first protrude, and 14- second protrudes, 16- carbon fibers diving heater wire, 17- support columns, the second screw knobs of 18-,
The second valves of 19-, the first valves of 20-.
Embodiment
The present invention is made further instructions below in conjunction with drawings and examples.
As depicted in figs. 1 and 2, ripple mixes the reactor for controlling algae in a kind of simulation provided by the invention, by interior case and constant temperature
Water bath two parts form.Interior case by the algae culture case of black, aerator, temperature measurement control system, top LED light
Band and the SCM system of its intensity of illumination is controlled to form, when LED light strip can simulate the vertical reciprocating motion of algae under interior wavy state
The change of the intensity of illumination of receiving.Constant water bath box is opened by transparent gallery shape water tank, carbon fiber diving heater wire and its control
The composition such as pass.Interior case is connected with constant water bath box by screwed pipe, and temperature controllable, dissolved oxygen concentration are unrestricted for algal grown
The growth characteristics and adaptation mechanism of algae under the conditions of sexual factor, the only cyclic fluctuation of quantitative study intensity of illumination, with quantitative simulation
Control effect and mechanism of the actual intensity of illumination cyclically-varying being layered in the storehouse of lake under interior wavy state of research to algal grown.
SCM system is independent design, is the electronic dimming controller using microcontroller as core control element, by monolithic
Mechanical, electrical machine drive module, DC power supply, dimming driver etc. are formed.Microcontroller is the core of whole system, LED light strip illumination
The adjusting of intensity is realized by mcu programming, and the program of the accounting by writing PWM ripples at different moments, can reach
Change the purpose of intensity of illumination at different moments.Motor drive module rise conversion dimming driver (0~10V) with microcontroller (0~
The effect of voltage between 5V).DC power supply is that alternating current is changed into direct current, and power supply is provided for whole circuit, is driven with light modulation
Dynamic device input terminal connection.Dimming driver is connected with DC power supply, its analog signals port being furnished with and motor drive module
Connection, it is by receiving the signal from microcontroller come the accounting of the PWM ripples of control output end mouthful, and so as to fulfill adjusting, illumination is strong
Degree, dimming scope 0%-100%, its output terminal and LED light band connection.
The constant water bath box is by transparent gallery shape water tank, the carbon fiber diving group such as heater wire and its controlling switch
Into, by be arranged in interior case 2 metal temperature probe 6 in real time monitoring reactors in water temperature, water temperature information is shown in electricity in time
Sub- temperature controlled shroud 5, makes temperature intuitively visible；And real-time water temperature information is fed back to and is arranged on below electron temperature control flow 5
Control unit, according to the constant water temperature being previously set in reactor, be turned on and off temperature detect switch (TDS) 1 to control carbon fiber to dive under water
The working status of heater wire, in real time and accurately controls constant water temperature in reactor.
Aerator is made of electric timing switch and aeration pump, in time in monitoring in case algae solution dissolved oxygen concentration, lead to
The separate run times and open frequency of control aeration pump are crossed, keep oxygen in water concentration 8mg/L or so, meet experiment algae
Growth requires.
Embodiment：
Whole device includes interior case and constant water bath box, is process with organic glass, and each major part is detachable,
In order to installation and maintenance.Interior case 2 is made of the black acrylic of 8 millimeters of thickness, and long 360mm, wide 320mm are high
Support column 17, high 80m, wide 40mm, 60 ° of inclination angle are arranged at 300mm, bottom.Light-emitting device by 3mm thickness cover board 7, SCM system
9th, the composition of LED light strip 8 (13), cover board 7 can cool downs to lamp, and ride over that the 30mm of interior case 2 highly locates first protrudes 13 and the
In two protrusions 14.There is the carbon fiber diving heater wire 16 to spiral between constant water bath box 4 and interior case 2, metal temperature probe 6 is real-time
Water temperature in case in monitoring, to first-class arrangement one on the parallel edges of another chamber；5 real-time display water of electron temperature control flow
Temperature, being turned on and off for temperature detect switch (TDS) 1 is determined according to the water temperature being previously set, and then controls the work of carbon fiber diving heater wire
State, in real time and accurately controls constant water temperature in reactor.Aeration pump 11 is controlled using time switch 1, according to timely prison
The dissolved oxygen concentration of survey determines its open frequency and separate run times, and the air that aeration pump 11 provides passes through uniform aeration first 12
Into interior case.Constant water bath box 4 is made of transparent organic glass, long 540mm, wide 520mm, high 350mm.Interior case 2 and constant temperature
Water bath 4 is connected with the concentric plastics ABS pipes of 20mm, and is equipped with screw knob and valve, and screw knob can facilitate two casees dismountings clear
Wash and dosing, the second valve 19 are drained for sluicing.
The interior ripple found according to certain stratified lake, it is respectively 3m, 7m there are the upper and lower depth of water, and intensity of illumination is respectively
9447lx, 3475lx, period of waves, 12h, using the reactor of ripple mixing control algae in a kind of simulation provided by the invention, was simulated
Obtained intensity of illumination is fluctuated as shown in figure 3, average light intensity is 6100lx in a cycle；It is respectively adopted as shown in Figure 3
Two kinds of intensity of illumination conditions of cyclic fluctuation intensity of illumination and constant illumination intensity 6100lx, have studied microcystic aeruginosa and grid
The growth course of algae, algae density (being represented in Fig. 4 with absorbance) is less than constant light under the conditions of finding cyclic fluctuation intensity of illumination
According to respective value under strength condition, wherein scenedesmus becomes apparent, and illustrates that cyclic fluctuation intensity of illumination can suppress to a certain extent
The growth of algae, also confirming that can use the present invention realize different cyclic fluctuation intensity of illumination conditions, ripple in analog study
Mix the suppression growth to algae and stress adaptation mechanism, and the Optimizing Technical of interior ripple mixing control algae.
Claims (9)
1. the reactor of ripple mixing control algae in a kind of simulation, it is characterised in that including constant water bath box (4) and be arranged on constant temperature
In water bath (4) and open-topped interior case (2)；Wherein,
The carbon fiber diving heater wire (16) to spiral in interior case (2) circumferential direction is provided with constant water bath box (4), and carbon fiber is dived
Water heater wire (16) is connected with being arranged on the temperature detect switch (TDS) (1) of constant water bath box (4) outside；
The opening of interior case (2) is provided with cover board (7), and cover board is provided with light-emitting device and aerator, and light-emitting device on (7)
Illumination towards in interior case (2), aerator is connected with the uniform aeration head (12) being arranged in interior case (2)；The side of interior case (2)
Wall has been sequentially communicated inlet tube and outlet tube from top to bottom, and inlet tube and outlet tube extend out to constant water bath box (4) outside.
2. the reactor of ripple mixing control algae in a kind of simulation according to claim 1, it is characterised in that cover board (7) is aluminium
Making sheet.
3. the reactor of ripple mixing control algae in a kind of simulation according to claim 1, it is characterised in that aerator includes
The aeration pump (11) and the time switch (10) of control aeration pump (11) work being arranged at the top of cover board (7), aeration pump (11)
Gas outlet connected with uniform aeration head (12).
4. the reactor of ripple mixing control algae in a kind of simulation according to claim 1, it is characterised in that in interior case (2) also
Metal temperature probe (6) is provided with, it is connected with being arranged on the electron temperature control flow (5) of constant water bath box (4) outside, electronics temperature
Degree control flow (5) is connected with temperature detect switch (TDS) (1), and temperature detect switch (TDS) (1) is connected with carbon fiber diving heater wire (16).
5. in a kind of simulation according to claim 1 ripple mixing control algae reactor, it is characterised in that interior case (2) it is interior
The first protrusion (13) and the second protrusion (14) are diagonally respectively arranged with wall, for limiting the installation site of cover board (7).
6. the reactor of ripple mixing control algae in a kind of simulation according to claim 1, it is characterised in that the bottom of interior case (2)
Portion corner is additionally provided with four support columns (17).
7. the reactor of ripple mixing control algae in a kind of simulation according to claim 1, it is characterised in that set on inlet tube
There are the first screw knob (3) and the first valve (20), the second screw knob (18) and the second valve (19) are provided with outlet tube.
8. the reactor of ripple mixing control algae in a kind of simulation according to claim 1, it is characterised in that light-emitting device includes
Be arranged on some LED light strip (8) of cover board (7) bottom, and set at the top of cover board (7) be used for adjust some LED light strip (8)
The SCM system (9) of intensity of illumination.
9. the reactor of ripple mixing control algae in a kind of simulation according to claim 8, it is characterised in that according to stratified lake
Typical case 5~30m of depth of water scope and interior 0.5~24h of wave period existing for ripple in reservoir, according to the index of intensity of illumination and the depth of water
Attenuation relation, calculates the Typical strengths and periodic regime of illumination under the conditions of the Ku Neibo of lake, is controlled using SCM system (9) some
The intensity of illumination of LED light strip (8) so that intensity of illumination is within the Typical strengths and periodic regime calculated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711423742.5A CN107904141B (en) | 2017-12-25 | 2017-12-25 | Reactor for simulating internal wave mixing algae control |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711423742.5A CN107904141B (en) | 2017-12-25 | 2017-12-25 | Reactor for simulating internal wave mixing algae control |
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| CN107904141B CN107904141B (en) | 2021-01-22 |
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