CN107903910B - For the biological agent of organic polluted soil reparation and its preparation, application method - Google Patents
For the biological agent of organic polluted soil reparation and its preparation, application method Download PDFInfo
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- CN107903910B CN107903910B CN201711136699.4A CN201711136699A CN107903910B CN 107903910 B CN107903910 B CN 107903910B CN 201711136699 A CN201711136699 A CN 201711136699A CN 107903910 B CN107903910 B CN 107903910B
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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Abstract
The invention discloses a kind of biological agents and its preparation and application for contaminated soil remediation.The ingredient of biological agent includes biosurfactant and solubilizer；Biosurfactant is with any 2 kinds in glycolipid, glucosides, cellulose, soya-bean oil, saponin, dextrin, starch, lignin or two or more mix with water and is constituted, and content of the above-mentioned each ingredient in biological agent is 2 ~ 80g/L；Solubilizer is sodium chloride, hydrochloric acid, sodium hydroxide, phosphate, any one or a few in nitrate；The pH value of said preparation is 6 ~ 9, and the concentration of salt is 2 ~ 50mol/L；Surface tension value is 15 ~ 45mN/m.Biological agent of the present invention extracts hydrophobic contaminant in soil, and activate the growth and activity of indigenous microorganism, to promote the degradation of organic pollutant by dispersion, solubilising, emulsification；And can with natural degradation, prevent secondary pollution.
Description
Technical field
The present invention relates to environmental protection technical fields, and in particular to a kind of biological agent for contaminated soil remediation and its
Preparation, application method, the biological agent can extract hydrophobic organic compound in soil and promote its biodegrade.
Background technique
For a long time, the organic matters such as petroleum, pesticide overflow and leakage can cause soil pollution, first after the leakage of these pollutants
Aeration zone is first penetrated into, is migrated under the interaction of capillary force and gravity, and as pollution sources long-term existence, it is serious broken
Bad soil primal environment poisons edaphon, reduces soil quality, influences agricultural production, or even underground water can be threatened safe,
Influence human health.
Effect of the surfactant in soil oily substance pollution amelioration mainly passes through from the absorption row on soil
To form different absorption and dissolution phase, solubilising or enhancing suction-operated being generated to oil pollutant, to change oil pollutant
Absorption behavior and bioavailability on soil, to reach reparation purpose.
But the problem of chemically synthesized surfactant is due to its raw material sources, price, properties of product etc., especially
It is that may cause secondary pollution, and be difficult to natural degradation, is subject to certain restrictions its application.
Summary of the invention
The high and environment amenable life for contaminated soil remediation the purpose of the present invention is to provide a kind of remediation efficiency
Object preparation and its preparation, application method.The purpose of the present invention is what is be achieved through the following technical solutions:
A kind of biological agent for contaminated soil remediation, the ingredient of the biological agent include biosurfactant and
Solubilizer；The biosurfactant is in glycolipid, glucosides, cellulose, soya-bean oil, saponin, dextrin, starch, lignin
Any 2 kinds or two or more composition is mixed with water, any other a kind of ingredient in biosurfactant is in biology than water
Content in preparation is 2 ~ 80g/L；The solubilizer is sodium chloride, hydrochloric acid, sodium hydroxide, phosphate, any in nitrate
It is one or more of；The pH value of the biological agent is 6 ~ 9, and the concentration of salt is 2 ~ 50mol/L in the biological agent；The biology
Dosage surface tension value is 15 ~ 45mN/m.
Further, the critical micelle concentration of the biological agent are as follows: 20-800mg/L.
Further, content of any other a kind of ingredient in biological agent in biosurfactant than water
For 2 ~ 45g/L.
Further, content of any other a kind of ingredient in biological agent in biosurfactant than water
For 15 ~ 35g/L.
Further, the biosurfactant is glycolipid, glucosides is mixed with water in composition or glycolipid, glucosides
At least one of at least one and cellulose, soya-bean oil, saponin, dextrin, starch, lignin mix composition with water.
The preparation method of the biological agent, comprising the following steps: 1) configure the biosurfactant；2) Xiang Sheng
Solubilizer is added in object surfactant to adjust the pH value and salinity of solution.
The application method of the biological agent: 1) by the well water at the biological agent scene or river water dilution 100 ~
10000 times；2) biological agent after diluting is applied in soil according to the ratio with 1 ~ 10:1 of soil volume ratio, keeps soil profit
Wet condition, and it is interrupted aeration, interval was mixed, with soil effect 30 days or more.
The beneficial effects of the present invention are:
1) biological agent of the invention is formulated by a certain percentage by biosurfactant and solubilizer, biological agent
Hydrophobic contaminant in soil is extracted by dispersion, solubilising, emulsification, forms stable oil-in-water emulsion, and activate soil
The growth and activity for writing microorganism, to promote the degradation process of the organic pollutants such as aliphatic, aromatic hydrocarbon, organochlorine.
2) biological agent can avoid introduce novel bacteria species and caused by biotic environment risk.
3) biological agent overcome synthetic surfactant only so that pollutant is scattered, cannot biodegrade, pollutant
The defect that do not eliminate finally.
4) biological agent can natural degradation, not will cause secondary pollution；It is that natural biology generates, is nontoxic, cheap
Environmentally friendly biological agent.
Specific embodiment
Embodiment 1
A kind of biological agent for contaminated soil remediation, ingredient include biosurfactant and solubilizer；The life
Object surfactant be with any 2 kinds or 2 kinds in glycolipid, glucosides, cellulose, soya-bean oil, saponin, dextrin, starch, lignin with
Upper that composition is mixed with water, content of any other a kind of ingredient in biological agent in biosurfactant is than water
2 ~ 80g/L, preferably 2 ~ 45g/L, more preferably 15 ~ 35g/L；The solubilizer is sodium chloride, hydrochloric acid, sodium hydroxide, phosphoric acid
Any one or a few in salt, nitrate；The pH value of the biological agent is 6 ~ 9, and the concentration of salt is 2 in the biological agent
~50mol/L；The biological agent surface tension value is 15 ~ 45mN/m, the critical micelle concentration of the biological agent are as follows: 20-
800mg/L。
Biosurfactant be preferably glycolipid, glucosides mixed with water at least one of composition or glycolipid, glucosides and
At least one of cellulose, soya-bean oil, saponin, dextrin, starch, lignin mix composition with water.
The preparation method of the biological agent, it is characterised in that: the following steps are included: 1) configuring the Bio-surface active
Agent；2) solubilizer is added into biosurfactant to adjust the pH value and salinity of solution.
The application method of the biological agent, 1) by the well water at the biological agent scene or river water dilution 100 ~
10000 times；2) biological agent after diluting is applied in soil according to the ratio with 1 ~ 10:1 of soil volume ratio, keeps soil profit
Wet condition, and it is interrupted aeration, interval was mixed, with soil effect 30 days or more.
The biosurfactant that the present invention has selected several predetermined substances to constitute, it is raw compared with synthetic surfactant
Object surfactant has outstanding advantages: selectivity is wide, environmentally friendly；Huge and complicated chemical structure makes surface-active
It is stronger with emulsifying capacity；Molecular structure type multiplicity, has many special functional groups, specificity is strong；Raw material is wide in nature
General presence, it is cheap；It is made by typical " green " production technology such as fermentation.
Biological agent in the present invention is biological agent Suo Lai (SOLE), and the molecule in SOLE is by hydrophilic and hydrophobic group group
At hydrophilic group protrudes into formation monolayer oriented film in water in water, reduces the surface tension of water.When surface reaches saturation, table
Just in aqueous solution accumulated inside, hydrophobic group flocks together to form kernel face active agent molecule, and hydrophilic group is contacted with water phase outwardly,
Form micella.
Embodiment 2
The present embodiment is the improvement carried out on the basis of embodiment 1, part same as Example 1 in the present embodiment,
Content disclosed in embodiment 1 is please referred to be understood, 1 disclosure of embodiment should also be as the content as the present embodiment,
It is not repeated description herein.
The biosurfactant that glycolipid, soya-bean oil, water mix is prepared, addition solubilizer adjusts pH=8.5, and guarantees that salt is dense
Degree is 2.5mol/L, obtains biological agent Sole-A, and wherein glycolipid and soya-bean oil concentration are respectively 15g/L and 35g/L, and scene is with working as
Ground well water and river water dilute 200 times.
The biosurfactant that saponin, lignin, water mix is prepared, addition solubilizer adjusts pH=9.0, and guarantees salt
Concentration is 2.5mol/L, obtains biological agent Sole-B, and wherein saponin and lignin concentration are respectively 15g/L and 15g/L, scene
100 times are diluted with local well water and river water.
The biosurfactant that glycolipid, glucosides, water mix is prepared, addition solubilizer adjusts pH=7.5, and guarantees that salt is dense
Degree is 21mol/L, obtains biological agent Sole-C, and wherein glycolipid and glycoside concentration are respectively 20g/L and 40g/L, and scene is with working as
Ground well water and river water dilute 100 times.
The biosurfactant that glucosides, starch, water mix is prepared, addition solubilizer adjusts pH=6.0, and guarantees that salt is dense
Degree is 1.5mol/L, obtains biological agent Sole-D, and wherein glucosides and starch concentration are respectively 25g/L and 15g/L, and scene is with working as
Ground well water and river water dilute 100 times.
The biosurfactant that glucosides, soya-bean oil, water mix is prepared, addition solubilizer adjusts pH=7.0, and guarantees that salt is dense
Degree is 2.0mol/L, obtains biological agent Sole-E, and wherein glucosides and soya-bean oil concentration are respectively 15g/L and 30g/L, and scene is with working as
Ground well water and river water dilute 150 times.
3 pedotheques S1, S2, S3 are acquired in the Dagang Oilfield oil recovery factory of Tianjin, oil content is respectively 5.2%, 6.5%,
8.3%.Respectively by the preparation after five kinds of dilutions for causing Sole-E with soil volume ratio 1:1 sprinkling Sole-A, soil wetting shape is kept
State, and it is interrupted aeration.It is listed in the table below respectively at 30 days and 90 days sample detection oil content:
After five kinds of Sole preparation effects, contained oil is all reduced in soil, the oil of preparation Sole-A, Sole-C, Sole-E
Degradation effect is significant.
Embodiment 3
Respectively 10 grams of soil of 61mg/kg and 85mg/kg pesticide producing place containing chlorobenzene and toluene, in 500ml taper
In bottle.Surface water dilutes 150 times near 5 kinds of Sole preparation places in embodiment 1, respectively takes 50ml, is separately added into 5 and fills 10
In the 500ml conical flask of gram Polluted Soil, oscillation takes Soil K+adsorption, chlorobenzene and toluene removal rate respectively reach 35%-75% and 41%-
81%.Ground moistening is kept, interval stirs, and chlorobenzene and toluene removal rate improve in soil after 30 days, and the best preparation of effect makes chlorine
The reduction of the concentration of benzene and toluene respectively reaches 85% and 93%.
Embodiment 4
The biosurfactant that glycolipid, soya-bean oil, dextrin, glucosides are mixed with water is prepared, addition solubilizer adjusts pH=8.0,
And guarantee that salinity is 25mol/L, biological agent Sole-F is obtained, wherein glycolipid, soya-bean oil, dextrin, glycoside concentration are respectively
15g/L, 25g/L, 2g/L, 4g/L, scene dilute 200 times with local well water and river water.
3 pedotheques S1, S2, S3 are acquired, oil content is respectively 5.2%, 6.5%, 8.3%.Point containing chlorobenzene and toluene
It Wei not 61mg/kg and 85mg/kg.By the preparation after diluting with soil volume ratio 1:1 sprinkling Sole-F, soil is kept to soak shape
State, and it is interrupted aeration.Respectively at 30 days and 90 days sample detection oil content.30 days chlorobenzenes and toluene removal rate are respectively as follows: 25-
31% and 23-32%, 90 days chlorobenzenes and toluene removal rate are respectively as follows: 67-75%, 61-78%.
Claims (6)
1. a kind of biological agent for organic polluted soil reparation, it is characterised in that: the ingredient of the biological agent is by biology
Surfactant and solubilizer composition；The biosurfactant be with glycolipid, glucosides, cellulose, soya-bean oil, saponin, dextrin,
Any 2 kinds in starch, lignin or two or more composition is mixed with water, it is any one in biosurfactant than water
Content of the kind ingredient in biological agent is 2 ~ 80g/L；The solubilizer is sodium chloride, hydrochloric acid, sodium hydroxide, phosphate, nitre
Any one or a few in hydrochlorate；The pH value of the biological agent is 6 ~ 9, in the biological agent concentration of salt be 2 ~
25mol/L；The biological agent surface tension value is 15 ~ 45mN/m；The biological agent the preparation method comprises the following steps: 1) configuration described in
Biosurfactant；2) solubilizer is added into biosurfactant to adjust the pH value and salinity of solution.
2. biological agent according to claim 1, it is characterised in that: the critical micelle concentration of the biological agent are as follows: 20-
800mg/L。
3. biological agent according to claim 1, it is characterised in that: any one in biosurfactant than water
Content of the kind ingredient in biological agent is 2 ~ 45g/L.
4. biological agent according to claim 3, it is characterised in that: any one in biosurfactant than water
Content of the kind ingredient in biological agent is 15 ~ 35g/L.
5. biological agent according to claim 1, it is characterised in that: the biosurfactant be glycolipid, glucosides with
Water mixing constitutes or at least one of glycolipid, glucosides and cellulose, soya-bean oil, saponin, dextrin, starch, in lignin at least
One kind mixing composition with water.
6. the application method of biological agent described in any one of claim 1 ~ 5, it is characterised in that: 1) make the biology
The well water or river water at agent scene dilute 100 ~ 10000 times；2) dilute after biological agent according to 1 ~ 10:1 of soil volume ratio
Ratio be applied in soil, keep soil wetting state, and be interrupted aeration, interval is mixed, with soil effect 30 days with
On.
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