CN107903910A - It is a kind of for the biological agent of contaminated soil remediation and its preparation, application method - Google Patents
It is a kind of for the biological agent of contaminated soil remediation and its preparation, application method Download PDFInfo
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- CN107903910A CN107903910A CN201711136699.4A CN201711136699A CN107903910A CN 107903910 A CN107903910 A CN 107903910A CN 201711136699 A CN201711136699 A CN 201711136699A CN 107903910 A CN107903910 A CN 107903910A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- Engineering & Computer Science (AREA)
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Abstract
The invention discloses a kind of biological agent and its preparation and application for contaminated soil remediation.The component of biological agent includes biosurfactant and solubilizer；Biosurfactant be with any 2 kinds in glycolipid, glucosides, cellulose, soya-bean oil, saponin, dextrin, starch, lignin or two or more mix and formed with water, content of the above-mentioned each component in biological agent is 2 ~ 80g/L；Solubilizer is any one or a few in sodium chloride, hydrochloric acid, sodium hydroxide, phosphate, nitrate；The pH value of said preparation is 6 ~ 9, and the concentration of salt is 2 ~ 50mol/L；Surface tension value is 15 ~ 45mN/m.Biological agent of the present invention is by disperseing, solubilising, emulsification, extracts hydrophobic contaminant in soil, and activates growth and the activity of indigenous microorganism, so as to promote the degraded of organic pollution；And can with natural degradation, prevent secondary pollution.
Description
Technical field
The present invention relates to environmental protection technical field, and in particular to a kind of biological agent for contaminated soil remediation and its
Prepare, application method, which can extract hydrophobic organic compound in soil and promote it biodegradable.
Background technology
For a long time, the organic matter such as oil, pesticide overflows and leakage can cause soil pollution, first after the leakage of these pollutants
Aeration zone is first penetrated into, is migrated under the interaction of capillary force and gravity, and is used as pollution sources long-term existence, it is serious broken
Bad soil primal environment, poisons edaphon, reduces soil quality, influences agricultural production, or even can threateningly descend water security,
Influence health.
Effect of the surfactant in the reparation of soil oils Substances Pollution is mainly by from the absorption row on soil
To form different absorption and dissolving phase, solubilising or enhancing suction-operated being produced to oil pollutant, to change oil pollutant
Absorption behavior and bioavailability on soil, so as to reach reparation purpose.
But the problem of surfactant of chemical synthesis is due to its raw material sources, price, properties of product etc., especially
It is that possible cause secondary pollution, and is difficult to natural degradation, is subject to certain restrictions its application.
The content of the invention
The high and environment amenable life for contaminated soil remediation it is an object of the invention to provide a kind of remediation efficiency
Thing preparation and its preparation, application method.The purpose of the present invention is what is be achieved through the following technical solutions：
A kind of biological agent for contaminated soil remediation, the component of the biological agent include biosurfactant and
Solubilizer；The biosurfactant is with glycolipid, glucosides, cellulose, soya-bean oil, saponin, dextrin, starch, lignin
Any 2 kinds or two or more composition is mixed with water, any other a kind of component in biosurfactant is in biology than water
Content in preparation is 2~80g/L；The solubilizer is sodium chloride, any in hydrochloric acid, sodium hydroxide, phosphate, nitrate
It is one or more of；The pH value of the biological agent is 6~9, and the concentration of salt is 2~50mol/L in the biological agent；The life
Thing dosage surface tension value is 15~45mN/m.
Further, the critical micelle concentration of the biological agent is：20-800mg/L.
Further, content of any other a kind of component in biological agent in biosurfactant than water
For 2~45g/L.
Further, content of any other a kind of component in biological agent in biosurfactant than water
For 15~35g/L.
Further, the biosurfactant mixes in composition, or glycolipid, glucosides for glycolipid, glucosides with water
At least one of at least one and cellulose, soya-bean oil, saponin, dextrin, starch, lignin mix composition with water.
The preparation method of the biological agent, comprises the following steps：1) biosurfactant is configured；2) to life
Solubilizer is added in thing surfactant to adjust the pH value of solution and salinity.
The application method of the biological agent：1) by the well water at biological agent scene or river water dilution 100~
10000 times；2) dilute after biological agent according to soil volume ratio 1~10：1 ratio is applied in soil, keeps soil
Wetting state, and it is interrupted aeration, interval is mixed, and is acted on more than 30 days with soil.
The beneficial effects of the invention are as follows：
1) biological agent of the invention is formulated by a certain percentage by biosurfactant and solubilizer, biological agent
Hydrophobic contaminant in soil is extracted by scattered, solubilising, emulsification, forms stable oil-in-water emulsion, and activate soil
Growth and the activity of microorganism are write, so as to promote the degradation process of the organic pollutions such as aliphatic, aromatic hydrocarbon, organochlorine.
2) biological agent can avoid introduce novel bacteria species and caused by biotic environment risk.
3) biological agent overcome synthetic surfactant simply pollutant is scattered, cannot biodegradable, pollutant
The defects of not eliminating finally.
4) biological agent can natural degradation, secondary pollution will not be caused；It is that natural biology produces, is nontoxic, cheap
Environmentally friendly biological agent.
Embodiment
Embodiment 1
Biological agent of the kind for contaminated soil remediation, component include biosurfactant and solubilizer；The biology
Surfactant be with any 2 kinds in glycolipid, glucosides, cellulose, soya-bean oil, saponin, dextrin, starch, lignin or two or more
Composition is mixed with water, content of any other a kind of component in biological agent in biosurfactant is 2 than water
~80g/L, is preferably 2~45g/L, more preferably 15~35g/L；The solubilizer is sodium chloride, hydrochloric acid, sodium hydroxide, phosphorus
Any one or a few in hydrochlorate, nitrate；The pH value of the biological agent is 6~9, the concentration of salt in the biological agent
For 2~50mol/L；The biological agent surface tension value is 15~45mN/m, and the critical micelle concentration of the biological agent is：
20-800mg/L。
Biosurfactant is preferably that glycolipid, glucosides mix composition with water, or at least one of glycolipid, glucosides and
At least one of cellulose, soya-bean oil, saponin, dextrin, starch, lignin mix composition with water.
The preparation method of the biological agent, it is characterised in that：Comprise the following steps：1) Bio-surface active is configured
Agent；2) solubilizer is added into biosurfactant to adjust the pH value of solution and salinity.
The application method of the biological agent, 1) by the well water at biological agent scene or river water dilution 100~10000
Times；2) dilute after biological agent according to soil volume ratio 1~10：1 ratio is applied in soil, keeps soil wetting shape
State, and it is interrupted aeration, interval is mixed, and is acted on more than 30 days with soil.
The present invention has selected the biosurfactant that several predetermined substances are formed, raw compared with synthetic surfactant
Thing surfactant has outstanding advantages：Selectivity is wide, environmentally friendly；Huge and complicated chemical constitution causes surface-active
It is stronger with emulsifying capacity；Molecular structure type is various, has many special functional groups, and specificity is strong；Raw material is wide in nature
General presence, it is cheap；It is made by typical " green " production technology such as fermentation.
Biological agent in the present invention is biological agent Suo Lai (SOLE), and the molecule in SOLE is by hydrophilic and hydrophobic group group
Into hydrophilic group stretches into formation monolayer oriented film in water in water, reduces the surface tension of water.When surface reaches saturation, table
Just in aqueous solution accumulated inside, hydrophobic group flocks together to form kernel face active agent molecule, and hydrophilic group is in contact with water outwardly,
Form micella.
Embodiment 2
The present embodiment is the improvement carried out on the basis of embodiment 1, part same as Example 1 in the present embodiment,
The content disclosed in embodiment 1 is refer to be understood, 1 disclosure of embodiment should also be as the content as the present embodiment,
Description is not repeated herein.
Glycolipid, soya-bean oil, the biosurfactant of water mixing are prepared, addition solubilizer adjusts pH=8.5, and ensures that salt is dense
Spend for 2.5mol/L, obtain biological agent Sole-A, wherein glycolipid and soya-bean oil concentration is respectively 15g/L and 35g/L, and scene is with working as
Ground well water and river water dilute 200 times.
Saponin, lignin, the biosurfactant of water mixing are prepared, addition solubilizer adjusts pH=9.0, and ensures salt
Concentration is 2.5mol/L, obtains biological agent Sole-B, and wherein saponin and lignin concentration is respectively 15g/L and 15g/L, scene
100 times are diluted with local well water and river water.
Glycolipid, glucosides, the biosurfactant of water mixing are prepared, addition solubilizer adjusts pH=7.5, and ensures that salt is dense
Spend for 21mol/L, obtain biological agent Sole-C, wherein glycolipid and glycoside concentration is respectively 20g/L and 40g/L, and scene is with working as
Ground well water and river water dilute 100 times.
Glucosides, starch, the biosurfactant of water mixing are prepared, addition solubilizer adjusts pH=6.0, and ensures that salt is dense
Spend for 1.5mol/L, obtain biological agent Sole-D, wherein glucosides and starch concentration is respectively 25g/L and 15g/L, and scene is with working as
Ground well water and river water dilute 100 times.
Glucosides, soya-bean oil, the biosurfactant of water mixing are prepared, addition solubilizer adjusts pH=7.0, and ensures that salt is dense
Spend for 2.0mol/L, obtain biological agent Sole-E, wherein glucosides and soya-bean oil concentration is respectively 15g/L and 30g/L, and scene is with working as
Ground well water and river water dilute 150 times.
3 pedotheques S1, S2, S3 are gathered in the Dagang Oilfield oil recovery factory of Tianjin, oil content is respectively 5.2%, 6.5%,
8.3%.Press and soil volume ratio 1 respectively:1 sprinkling Sole-A causes the preparation after five kinds of dilutions of Sole-E, keeps soil wetting
State, and it is interrupted aeration.It is listed in the table below respectively at 30 days and 90 days sampling detection oil content：
After five kinds of Sole preparations effects, contained oil is all reduced in soil, the oil of preparation Sole-A, Sole-C, Sole-E
Degradation effect is notable.
Embodiment 3
Respectively 10 grams of 61mg/kg and 85mg/kg pesticide producings place soil containing chlorobenzene and toluene, in 500ml tapers
In bottle.Surface water dilutes 150 times near 5 kinds of Sole preparations places in embodiment 1, respectively takes 50ml, is separately added into 5 and fills 10
In the 500ml conical flasks of gram Polluted Soil, vibration takes Soil K+adsorption, chlorobenzene and toluene removal rate respectively reach 35%-75% and
41%-81%.Ground moistening is kept, interval stirs, and chlorobenzene and toluene removal rate improve in soil after 30 days, the best system of effect
Agent makes the reduction of the concentration of chlorobenzene and toluene respectively reach 85% and 93%.
Embodiment 4
The biosurfactant that glycolipid, soya-bean oil, dextrin, glucosides are mixed with water is prepared, addition solubilizer adjusts pH=
8.0, and ensure that salinity is 25mol/L, obtain biological agent Sole-F, wherein glycolipid, soya-bean oil, dextrin, glycoside concentration difference
For 15g/L, 25g/L, 2g/L, 4g/L, scene dilutes 200 times with local well water and river water.
3 pedotheques S1, S2, S3 are gathered, oil content is respectively 5.2%, 6.5%, 8.3%.Containing chlorobenzene and toluene
Respectively 61mg/kg and 85mg/kg.By with soil volume ratio 1:Preparation after 1 sprinkling Sole-F dilutions, keeps soil wetting shape
State, and it is interrupted aeration.Respectively at 30 days and 90 days sampling detection oil content.30 days chlorobenzenes and toluene removal rate are respectively：25-
31% and 23-32%, 90 days chlorobenzenes and toluene removal rate are respectively：67-75%, 61-78%.
Claims (7)
- A kind of 1. biological agent for contaminated soil remediation, it is characterised in that：The component of the biological agent includes biological table Face activating agent and solubilizer；The biosurfactant be with glycolipid, glucosides, cellulose, soya-bean oil, saponin, dextrin, starch, Any 2 kinds in lignin or two or more composition is mixed with water, any one component in biosurfactant than water Content in biological agent is 2 ~ 80g/L；The solubilizer is sodium chloride, in hydrochloric acid, sodium hydroxide, phosphate, nitrate Any one or a few；The pH value of the biological agent is 6 ~ 9, and the concentration of salt is 2 ~ 50mol/L in the biological agent；Institute It is 15 ~ 45mN/m to state biological agent surface tension value.
- 2. biological agent according to claim 1, it is characterised in that：The critical micelle concentration of the biological agent is：20- 800mg/L。
- 3. biological agent according to claim 1, it is characterised in that：It is any one in biosurfactant than water Content of the kind component in biological agent is 2 ~ 45g/L.
- 4. biological agent according to claim 3, it is characterised in that：It is any one in biosurfactant than water Content of the kind component in biological agent is 15 ~ 35g/L.
- 5. biological agent according to claim 1, it is characterised in that：The biosurfactant for glycolipid, glucosides with Water mixing is formed, or at least one of glycolipid, glucosides and cellulose, soya-bean oil, saponin, dextrin, starch, lignin at least One kind mixes composition with water.
- 6. the preparation method of the biological agent in claim 1 ~ 5 described in any one, it is characterised in that：Comprise the following steps： 1）Configure the biosurfactant；2）Solubilizer is added into biosurfactant to adjust the pH value of solution and salt Concentration.
- 7. the application method of the biological agent in claim 1 ~ 5 described in any one, it is characterised in that：1）The biology is made The well water or river water at agent scene dilute 100 ~ 10000 times；2）Biological agent after dilution according to soil volume ratio 1 ~ 10：1 Ratio be applied in soil, keep soil wetting state, and be interrupted aeration, interval is mixed, with soil effect 30 days with On.
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Cited By (1)
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| RU2814273C1 (en) * | 2023-06-27 | 2024-02-28 | Светлана Валерьевна Грошева | Method for decontamination of oil-contaminated soils |
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