CN107898678A - A kind of preparation method of plant hair conditioner - Google Patents

A kind of preparation method of plant hair conditioner Download PDF

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Publication number
CN107898678A
CN107898678A CN201711478442.7A CN201711478442A CN107898678A CN 107898678 A CN107898678 A CN 107898678A CN 201711478442 A CN201711478442 A CN 201711478442A CN 107898678 A CN107898678 A CN 107898678A
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seaweed
mass ratio
preparation
resin
obtains
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梁立明
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A61Q5/12—Preparations containing hair conditioners
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A61Q5/002—Preparations for repairing the hair, e.g. hair cure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

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  • Health & Medical Sciences (AREA)
  • Dermatology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Cosmetics (AREA)

Abstract

The present invention relates to a kind of preparation method of plant hair conditioner, it is by seaweed extracted liquor, surfactant, moisturizer, nourishing agent, thickener, pearling agent, essence, fungicide and water according to mass ratio 5~89:2~89:0~30:0~50:0.5~15:0~30:0~5:0~5:0~70 group of cost vegetable hair conditioner is made of seaweed extracted liquor, surfactant, moisturizer, nourishing agent, thickener, pearling agent, essence and water.The present invention has effects that to nourish healthy hair.

Description

A kind of preparation method of plant hair conditioner
Technical field
The present invention relates to a kind of preparation method of plant hair conditioner.
Background technology
The long-term scalp for lacking nourishing can allow the environment of natural on-off cycles of hair growth to become very poor, easily cause alopecia head to be itched, hair it is withered Yellow jag even grows the hair problems such as white hair.
The many shampoos of in the market are blent using chemokine class and formed, it is impossible to play profit hair hair care effect well.
The content of the invention
The technical problem to be solved by the invention is to provide a kind of preparation method of plant hair conditioner, has hair care hair care The effect of.
In order to solve the above-mentioned technical problem, the present invention uses following technical scheme：
A kind of preparation method of plant hair conditioner, it is characterised in that：
It is by seaweed extracted liquor, surfactant, moisturizer, nourishing agent, thickener, pearling agent, essence, fungicide and water According to mass ratio 5~89:2~89:0~30:0~50:0.5~15:0~30:0~5:0~5:0~70 composition；
The surfactant includes polyglyceryl fatty acid ester, lauric monoglyceride, methyl glycol fatty acid ester, sorbierite Acid anhydride monopalmitate, stearoyl lactate, diacetyl tartarate monoglyceride, Cocoamidopropyl betaine, polyoxyethylene nonyl phenyl Vinethene, polyoxyethylene oleic acid ester, polyoxyethylene stearic acid ester, sorbitan fatty acid ester, two polyglycereol, cetyl three Methyl quaternary ammonium ammonium bromide, octadecyl dimethyl benzyl quaternary ammonium ammonium chloride, benzyltriethylammoinium chloride, diisooctyl succinate One or any ratio in sodium sulfonate and aliphatic acid polyethenoxy fat it is two or more；
The moisturizer is glycerine, fructose, propane diols, sorbierite, polyglutamic acid, niacinamide, polyethylene glycol and xylose One or any ratio in alcohol it is two or more；
The nourishing agent include honey, sodium salicylate, superoxide dismutase, Alphalin, beta glucan, vitamin e, rely One or any ratio in propylhomoserin sodium and vitamin E it is two or more；
The thickener include sodium chloride, sodium carboxymethylcellulose, gelatin, sodium alginate and casein in one kind or Arbitrary proportion it is two or more；
The pearling agent includes one kind in pearl powder, stearic acid diethylene glycol dilaurate, ethylene glycol double stearic acid and mica powder Or arbitrary proportion is two or more；
The fungicide is included in methylisothiazolinone, methylchloroisothiazandnone, sodium benzoate and potassium sorbate One or any ratio it is two or more；
The seaweed extracted liquor, follows the steps below：
1) seaweed fragment, is prepared：Seaweed after cleaning is crushed, obtains seaweed fragment；
2) enzymolysis seaweed, is prepared：Seaweed fragment and water are fitted into agitator tank, under the conditions of 60~120 DEG C sterilize 5~ 40min, is cooled to 27~45 DEG C of access enzymes, digests 30~60min, the mass ratio 5~30 of seaweed fragment, water and enzyme:70~ 95:0.02~1, seaweed must be digested；
3) seaweed filtrate and seaweed slag, are prepared：Enzymolysis seaweed is separated, gained filtrate is seaweed filtrate, residue obtained to be Seaweed slag；
4) fermentation of seaweed thing, is prepared：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to Mass ratio 95.5:1:0.5:1:1:1 is added in fermentation tank and mixes, and water content is adjusted to water as 50~70%, at 70~150 DEG C Under the conditions of sterilize 5~40min, be cooled to 27~36 DEG C, obtain mixed culture medium, access rhodotorula mucilaginosa, rhodotorula mucilaginosa and mixing are trained The mass ratio for supporting base is 0.1~2:98~99.9, it is 27~36 DEG C in temperature, oxygen concentration is 8~21% in fermentation tank, fermentation 6~144h, sterilize 20~40min under the conditions of being 70~150 DEG C in temperature, is cooled to 20~40 DEG C, obtains fermentation of seaweed thing；
5) seaweed extracted liquor and seaweed residue, are prepared：The seaweed filtrate that step 3) obtains is added to what step 4) obtained Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method The mass ratio of corynebacterium ammoniagenes is 95~99.8:0.2~5, it is 25~35 DEG C in temperature, under the conditions of oxygen content is 1~8mg/L, Fermentation 1~6 day, gained filtrate is seaweed extracted liquor after press filtration；Filter residue is seaweed residue；
The seaweed is that one kind in Enteromorpha, kelp, sargassum, opotism and kelp or arbitrary proportion are two or more.
The preparation of thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for Strain；
B) thawing method improves the permeability of cell：Thawing method is at least repeated once, its step is：The expansion that step a) is obtained It is further cultured for strain greatly to be put into -20 DEG C of refrigerator, freezes 4h, takes out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone 10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
The enzyme is lysozyme and pectase according to mass ratio 5:1 composition, wherein lysozyme according to following steps into OK：
(A) add the water of 5 times of weight to egg white or whole egg liquid, after stirring evenly add homogenizer in, at least it is homogeneous once, it is even Matter temperature is 30~60 DEG C, and homogeneous pressure is 2~40MPa, and the homogeneous time is 10~40min, is filtered after homogeneous, gained filtrate is Dilution；
(B) the dilution molecular cut off for obtaining step (A) is the ultrafiltration membrane ultrafiltration of 15000~30000Da, is obtained Thin liquid and dope；
(C) add resin in the thin liquid that step (B) obtains to be adsorbed, the part by weight of the thin liquid and resin is 1: 3~8, the resin after being adsorbed；
(D) resin after the absorption for obtaining step (C) is eluted with the NaCl aqueous solutions of 0.001~0.5mol/L, Obtain eluent；
(E) by the eluent that step (D) obtains ultrafiltration membrane ultrafiltration desalination, ultrafiltrate, the retention of the ultrafiltration membrane are obtained Molecular weight is 2000~10000Da；
(F) after the ultrafiltrate drying obtained step (E), that is, lysozyme is obtained；
The resin is that the resin is cation exchange resin or macroporous absorbent resin；The model of cation exchange resin For 001*7,732, AmberliteIR-120, Dowex-50, Lewatit-100, Diaion SK-1, AllassionCS, One or more in Duolite C-20, SDB-3, macroporous absorbent resin AB-8, D101, D3520, X-5, NKA-II, One or more in NKA-9, S-8, XDA-1, H-20, H-30, H-40.
Step 3) the separation is included more than one or both of press filtration, filtering and centrifugation.
Step 4) the mixed culture medium to water content is 60%, and the mass ratio of rhodotorula mucilaginosa and mixed culture medium is 0.5: 99.5, it is 30 DEG C in temperature, oxygen concentration 12%, ferment 120h, and sterilize 20min under the conditions of being 120 DEG C in temperature.
The mass ratio of corynebacterium ammoniagenes prepared by step 5) mixture and thawing method is 98:2, it is 28 DEG C in temperature, it is oxygen-containing Measure under the conditions of 3mg/L, to ferment 5 days.
The rhodotorula mucilaginosa and corynebacterium ammoniagenes are purchased from Chinese industrial Microbiological Culture Collection administrative center, numbering difference For CICC 31192 and CICC 10168.
Invention has following advantageous effects：
1st, the present invention use new fresh seaweed, seaweed growth in high salt, cold seawater, itself have well self Defencive function, and contain a large amount of amino acid in seaweed, there is tonic effect to healthy hair.
2nd, the present invention pre-processes seaweed using lysozyme and pectase, and lysozyme makes the insoluble glutinous polysaccharide of cell membrane Soluble glycopeptide is resolved into, causes cell wall rupture content to escape, facilitates subsequent extracted.
3rd, the present invention is fermented under anoxic conditions using aerobic and anaerobism corynebacterium ammoniagenes, produces ATP, and ATP is a kind of High energy phosphate compound, in cell, it can realize energy storage and exoergic with mutually converting for ADP, so as to ensure that cell items The energy supply of vital movement, forms healthy hair and nourishes and repair damaged scalp.
4th, the permeability of corynebacterium ammoniagenes cell is improved by thawing method, the ATP that corynebacterium ammoniagenes produce can be made more to hold Extracellular, product of the product effects significantly better than undressed corynebacterium ammoniagenes is easily penetrated into from cell membrane.
Embodiment
The present invention is further illustrated with reference to instantiation.
Embodiment 1
Plant hair conditioner by seaweed extracted liquor, surfactant, moisturizer, nourishing agent, thickener, pearling agent, essence, kill Microbial inoculum and water are according to mass ratio 35:5:8:10:0.5:3:0.5:0.5:37.5 compositions.The surfactant is polyglycereol fat Acid esters, lauric monoglyceride and cetyl trimethyl quaternary ammonium ammonium bromide are according to mass ratio 3:2:1 composition；The moisturizing Agent is glycerine, fructose, propane diols, sorbierite and polyglutamic acid according to mass ratio 1:1:2:1:1 composition；The nourishing agent It is honey, superoxide dismutase, Alphalin, vitamin e and vitamin E according to mass ratio 1:3:2:1:1 composition；Institute It is sodium alginate and sodium chloride according to mass ratio 1 to state thickener:2 composition；The pearling agent be the double stearic acid of ethylene glycol and Stearic acid diethylene glycol dilaurate is according to mass ratio 1:2 composition；The fungicide be methylisothiazolinone and potassium sorbate by According to mass ratio 1:1 composition.
The preparation process of seaweed extracted liquor, follows the steps below：
1) seaweed fragment, is prepared：Fresh Laminaria Japonica after cleaning is crushed, obtains seaweed fragment；
2) enzymolysis seaweed, is prepared：Seaweed fragment and water are fitted into agitator tank, sterilize 15min under the conditions of 100 DEG C, 30 DEG C of access enzymes are cooled to, digest 40min, the mass ratio 20 of seaweed fragment, water and enzyme:79.94:0.06, seaweed must be digested, The enzyme is lysozyme and pectase according to mass ratio 5:1 composition；
3) seaweed filtrate and seaweed slag, are prepared：The press filtration of enzymolysis seaweed is separated, gained filtrate is seaweed filtrate, and gained is residual Slag is seaweed slag；
4) fermentation of seaweed thing, is prepared：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to Mass ratio 95.5:1:0.5:1:1:1 is added in fermentation tank and mixes, and is adjusted to water content as 60% with water, goes out under the conditions of 120 DEG C Bacterium 20min, is cooled to 30 DEG C, obtains mixed culture medium, accesses rhodotorula mucilaginosa, and the mass ratio of rhodotorula mucilaginosa and mixed culture medium is 0.5:99.5, it is 30 DEG C in temperature, oxygen concentration is 12% in fermentation tank, and ferment 120h, is sterilized under the conditions of being 120 DEG C in temperature 20min, is cooled to 35 DEG C, obtains fermentation of seaweed thing；
5) seaweed extracted liquor and seaweed residue, are prepared：The seaweed filtrate that step 3) obtains is added to what step 4) obtained Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method The mass ratio of corynebacterium ammoniagenes is 98:2, it is 28 DEG C in temperature, under the conditions of oxygen content is 3mg/L, ferments 5 days, press filtration, gained filter Liquid is seaweed extracted liquor；Filter residue is seaweed residue；
The preparation of wherein thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for Strain；
B) thawing method improves the permeability of cell：In triplicate, its step is thawing method：The expansion that step 1) is obtained is again Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved, be placed again into -20 DEG C of refrigerator at room temperature, is freezed 4h, takes out, dissolves, be placed again into -20 DEG C of refrigerator at room temperature, freezes 4h, takes out, dissolves at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone 10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Wherein lysozyme follows the steps below：
(A) add the water of 5 times of weight to egg white or whole egg liquid, added after stirring evenly in homogenizer, homogeneous 2 times, homogeneous temperature Degree is 40 DEG C, and the homogeneous time is 15min, and wherein first time homogeneous pressure is 25MPa, and second of homogeneous pressure is 4MPa, Filtering after homogeneous, gained filtrate is dilution；
(B) the ultrafiltration membrane ultrafiltration for being 28000Da with molecular cut off by the dilution that step (A) obtains, obtain thin liquid and Dope；
(C) add resin in the thin liquid that step (B) obtains to be adsorbed, the part by weight of the thin liquid and resin is 1: 5, the resin after being adsorbed；
(D) resin after the absorption for obtaining step (C) is eluted with the NaCl aqueous solutions of 0.003mol/L, is washed De- liquid；
(E) by the eluent that step (D) obtains ultrafiltration membrane ultrafiltration desalination, ultrafiltrate, the retention of the ultrafiltration membrane are obtained Molecular weight is 8000Da；
(F) after the ultrafiltrate drying obtained step (E), that is, lysozyme is obtained.
Embodiment 2
Plant hair conditioner by seaweed extracted liquor, surfactant, moisturizer, nourishing agent, thickener, pearling agent, essence, kill Microbial inoculum and water are according to mass ratio 35:5:8:10:0.5:3:0.5:0.5:37.5 compositions.The surfactant is polyglycereol fat Acid esters, lauric monoglyceride and cetyl trimethyl quaternary ammonium ammonium bromide are according to mass ratio 3:2:1 composition；The moisturizing Agent is glycerine, fructose, propane diols, sorbierite and polyglutamic acid according to mass ratio 1:1:2:1:1 composition；The nourishing agent It is honey, superoxide dismutase, Alphalin, vitamin e and vitamin E according to mass ratio 1:3:2:1:1 composition；Institute It is sodium alginate and sodium chloride according to mass ratio 1 to state thickener:2 composition；The pearling agent be the double stearic acid of ethylene glycol and Stearic acid diethylene glycol dilaurate is according to mass ratio 1:2 composition；The fungicide be methylisothiazolinone and potassium sorbate by According to mass ratio 1:1 composition.
The preparation process of seaweed extracted liquor, follows the steps below：
1) seaweed fragment, is prepared：Fresh Laminaria Japonica after cleaning is crushed, obtains seaweed fragment；
2) enzymolysis seaweed, is prepared：Seaweed fragment and water are fitted into agitator tank, sterilize 15min under the conditions of 100 DEG C, 30 DEG C of access enzymes are cooled to, digest 40min, the mass ratio 20 of seaweed fragment, water and enzyme:79.94:0.06, seaweed must be digested, The enzyme is lysozyme and pectase according to mass ratio 5:1 composition；
3) seaweed filtrate and seaweed slag, are prepared：The press filtration of enzymolysis seaweed is separated, gained filtrate is seaweed filtrate, and gained is residual Slag is seaweed slag；
4) fermentation of seaweed thing, is prepared：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to Mass ratio 95.5:1:0.5:1:1:1 is added in fermentation tank and mixes, and is adjusted to water content as 60% with water, goes out under the conditions of 120 DEG C Bacterium 20min, is cooled to 30 DEG C, obtains mixed culture medium, accesses rhodotorula mucilaginosa, and the mass ratio of rhodotorula mucilaginosa and mixed culture medium is 0.5:99.5, it is 30 DEG C in temperature, oxygen concentration is 12% in fermentation tank, and ferment 120h, is sterilized under the conditions of being 120 DEG C in temperature 20min, is cooled to 35 DEG C, obtains fermentation of seaweed thing；
5) seaweed extracted liquor and seaweed residue, are prepared：The seaweed filtrate that step 3) obtains is added to what step 4) obtained Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method The mass ratio of corynebacterium ammoniagenes is 98:2, it is 28 DEG C in temperature, under the conditions of oxygen content is 3mg/L, ferments 5 days, press filtration, gained filter Liquid is seaweed extracted liquor；Filter residue is seaweed residue；
The preparation of wherein thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for Strain；
B) thawing method improves the permeability of cell：In triplicate, its step is thawing method：The expansion that step 1) is obtained is again Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved, be placed again into -20 DEG C of refrigerator at room temperature, is freezed 4h, takes out, dissolves, be placed again into -20 DEG C of refrigerator at room temperature, freezes 4h, takes out, dissolves at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone 10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Wherein lysozyme follows the steps below：
(A) add the water of 5 times of weight to egg white or whole egg liquid, added after stirring evenly in homogenizer, homogeneous 2 times, homogeneous temperature Degree is 35 DEG C, and the homogeneous time is 15min, and wherein first time homogeneous pressure is 25MPa, and second of homogeneous pressure is 4MPa, Filtering after homogeneous, gained filtrate is dilution；
(B) the ultrafiltration membrane ultrafiltration for being 28000Da with molecular cut off by the dilution that step (A) obtains, obtain thin liquid and Dope；
(C) add resin in the thin liquid that step (B) obtains to be adsorbed, the part by weight of the thin liquid and resin is 1: 5, the resin after being adsorbed；
(D) resin after the absorption for obtaining step (C) is eluted with the NaCl aqueous solutions of 0.003mol/L, is washed De- liquid；
(E) by the eluent that step (D) obtains ultrafiltration membrane ultrafiltration desalination, ultrafiltrate, the retention of the ultrafiltration membrane are obtained Molecular weight is 8000Da；
(F) after the ultrafiltrate drying obtained step (E), that is, lysozyme is obtained.
Embodiment 3
Plant hair conditioner by seaweed extracted liquor, surfactant, moisturizer, nourishing agent, thickener, pearling agent, essence, kill Microbial inoculum and water are according to mass ratio 60:6:5:5:1:5:0.5:0.5:17 compositions.The surfactant be polyglyceryl fatty acid ester, Lauric monoglyceride and cetyl trimethyl quaternary ammonium ammonium bromide are according to mass ratio 3:2:1 composition；The moisturizer is It is glycerine, fructose, propane diols, sorbierite and polyglutamic acid according to mass ratio 1:1:2:1:1 composition；The nourishing agent is bee Honey, superoxide dismutase, Alphalin, vitamin e and vitamin E are according to mass ratio 1:3:2:1:1 composition；The increasing Thick dose is sodium alginate and sodium chloride according to mass ratio 1:2 composition；The pearling agent is the double stearic acid of ethylene glycol and tristearin Sour diethylene glycol dilaurate is according to mass ratio 1:2 composition；The fungicide is methylisothiazolinone and potassium sorbate according to matter Measure ratio 1:1 composition.
The preparation process of seaweed extracted liquor, follows the steps below：
1) seaweed fragment, is prepared：Fresh Laminaria Japonica after cleaning is crushed, obtains seaweed fragment；
2) enzymolysis seaweed, is prepared：Seaweed fragment and water are fitted into agitator tank, sterilize 15min under the conditions of 100 DEG C, 30 DEG C of access enzymes are cooled to, digest 40min, the mass ratio 20 of seaweed fragment, water and enzyme:79.94:0.06, seaweed must be digested, The enzyme is lysozyme and pectase according to mass ratio 5:1 composition；
3) seaweed filtrate and seaweed slag, are prepared：The press filtration of enzymolysis seaweed is separated, gained filtrate is seaweed filtrate, and gained is residual Slag is seaweed slag；
4) fermentation of seaweed thing, is prepared：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to Mass ratio 95.5:1:0.5:1:1:1 is added in fermentation tank and mixes, and is adjusted to water content as 60% with water, goes out under the conditions of 120 DEG C Bacterium 20min, is cooled to 30 DEG C, obtains mixed culture medium, accesses rhodotorula mucilaginosa, and the mass ratio of rhodotorula mucilaginosa and mixed culture medium is 0.5:99.5, it is 30 DEG C in temperature, oxygen concentration is 12% in fermentation tank, and ferment 120h, is sterilized under the conditions of being 120 DEG C in temperature 20min, is cooled to 35 DEG C, obtains fermentation of seaweed thing；
5) seaweed extracted liquor and seaweed residue, are prepared：The seaweed filtrate that step 3) obtains is added to what step 4) obtained Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method The mass ratio of corynebacterium ammoniagenes is 98:2, it is 28 DEG C in temperature, under the conditions of oxygen content is 3mg/L, ferments 5 days, press filtration, gained filter Liquid is seaweed extracted liquor；Filter residue is seaweed residue；
The preparation of wherein thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for Strain；
B) thawing method improves the permeability of cell：In triplicate, its step is thawing method：The expansion that step 1) is obtained is again Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved, be placed again into -20 DEG C of refrigerator at room temperature, is freezed 4h, takes out, dissolves, be placed again into -20 DEG C of refrigerator at room temperature, freezes 4h, takes out, dissolves at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone 10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Wherein lysozyme follows the steps below：
(A) add the water of 5 times of weight to egg white or whole egg liquid, added after stirring evenly in homogenizer, homogeneous 2 times, homogeneous temperature Degree is 45 DEG C, and the homogeneous time is 15min, and wherein first time homogeneous pressure is 25MPa, and second of homogeneous pressure is 4MPa, Filtering after homogeneous, gained filtrate is dilution；
(B) the ultrafiltration membrane ultrafiltration for being 28000Da with molecular cut off by the dilution that step (A) obtains, obtain thin liquid and Dope；
(C) add resin in the thin liquid that step (B) obtains to be adsorbed, the part by weight of the thin liquid and resin is 1: 5, the resin after being adsorbed；
(D) resin after the absorption for obtaining step (C) is eluted with the NaCl aqueous solutions of 0.003mol/L, is washed De- liquid；
(E) by the eluent that step (D) obtains ultrafiltration membrane ultrafiltration desalination, ultrafiltrate, the retention of the ultrafiltration membrane are obtained Molecular weight is 8000Da；
(F) after the ultrafiltrate drying obtained step (E), that is, lysozyme is obtained.
Embodiment 4
Plant hair conditioner by seaweed extracted liquor, surfactant, moisturizer, nourishing agent, thickener, pearling agent, essence, kill Microbial inoculum and water are according to mass ratio 40:20:15:8:1:10:0.5:0.5:5 compositions.The surfactant is polyglycerol fatty acid Ester, lauric monoglyceride and cetyl trimethyl quaternary ammonium ammonium bromide are according to mass ratio 3:2:1 composition；The moisturizer It is glycerine, fructose, propane diols, sorbierite and polyglutamic acid according to mass ratio 1:1:2:1:1 composition；The nourishing agent is Honey, superoxide dismutase, Alphalin, vitamin e and vitamin E are according to mass ratio 1:3:2:1:1 composition；It is described Thickener is sodium alginate and sodium chloride according to mass ratio 1:2 composition；The pearling agent is the double stearic acid of ethylene glycol and hard Resin acid diethylene glycol dilaurate is according to mass ratio 1:2 composition；The fungicide be methylisothiazolinone and potassium sorbate according to Mass ratio 1:1 composition.
The preparation process of seaweed extracted liquor, follows the steps below：
1) seaweed fragment, is prepared：Fresh Laminaria Japonica after cleaning is crushed, obtains seaweed fragment；
2) enzymolysis seaweed, is prepared：Seaweed fragment and water are fitted into agitator tank, sterilize 15min under the conditions of 100 DEG C, 30 DEG C of access enzymes are cooled to, digest 40min, the mass ratio 20 of seaweed fragment, water and enzyme:79.94:0.06, seaweed must be digested, The enzyme is lysozyme and pectase according to mass ratio 5:1 composition；
3) seaweed filtrate and seaweed slag, are prepared：The press filtration of enzymolysis seaweed is separated, gained filtrate is seaweed filtrate, and gained is residual Slag is seaweed slag；
4) fermentation of seaweed thing, is prepared：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to Mass ratio 95.5:1:0.5:1:1:1 is added in fermentation tank and mixes, and is adjusted to water content as 60% with water, goes out under the conditions of 120 DEG C Bacterium 20min, is cooled to 30 DEG C, obtains mixed culture medium, accesses rhodotorula mucilaginosa, and the mass ratio of rhodotorula mucilaginosa and mixed culture medium is 0.5:99.5, it is 30 DEG C in temperature, oxygen concentration is 12% in fermentation tank, and ferment 120h, is sterilized under the conditions of being 120 DEG C in temperature 20min, is cooled to 35 DEG C, obtains fermentation of seaweed thing；
5) seaweed extracted liquor and seaweed residue, are prepared：The seaweed filtrate that step 3) obtains is added to what step 4) obtained Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method The mass ratio of corynebacterium ammoniagenes is 98:2, it is 28 DEG C in temperature, under the conditions of oxygen content is 3mg/L, ferments 5 days, press filtration, gained filter Liquid is seaweed extracted liquor；Filter residue is seaweed residue；
The preparation of wherein thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for Strain；
B) thawing method improves the permeability of cell：In triplicate, its step is thawing method：The expansion that step 1) is obtained is again Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved, be placed again into -20 DEG C of refrigerator at room temperature, is freezed 4h, takes out, dissolves, be placed again into -20 DEG C of refrigerator at room temperature, freezes 4h, takes out, dissolves at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone 10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Wherein lysozyme follows the steps below：
(A) add the water of 5 times of weight to egg white or whole egg liquid, added after stirring evenly in homogenizer, homogeneous 1 time, homogeneous temperature Degree is 40 DEG C, and the homogeneous time is 30min, and homogeneous pressure 25MPa, is filtered, gained filtrate is dilution after homogeneous；
(B) the ultrafiltration membrane ultrafiltration for being 28000Da with molecular cut off by the dilution that step (A) obtains, obtain thin liquid and Dope；
(C) add resin in the thin liquid that step (B) obtains to be adsorbed, the part by weight of the thin liquid and resin is 1: 5, the resin after being adsorbed；
(D) resin after the absorption for obtaining step (C) is eluted with the NaCl aqueous solutions of 0.003mol/L, is washed De- liquid；
(E) by the eluent that step (D) obtains ultrafiltration membrane ultrafiltration desalination, ultrafiltrate, the retention of the ultrafiltration membrane are obtained Molecular weight is 8000Da；
(F) after the ultrafiltrate drying obtained step (E), that is, lysozyme is obtained.
Embodiment 5
Plant hair conditioner is by seaweed extracted liquor, surfactant and thickener according to mass ratio 80:19.5:0.5 composition.Institute It is polyglyceryl fatty acid ester and cetyl trimethyl quaternary ammonium ammonium bromide according to mass ratio 2 to state surfactant:1 composition； The moisturizer is glycerine；The nourishing agent is honey and superoxide dismutase according to mass ratio 1:1 composition；It is described Thickener is sodium chloride；The pearling agent is the double stearic acid of ethylene glycol and stearic acid diethylene glycol dilaurate according to mass ratio 1:2 group Compound；The fungicide is methylisothiazolinone and potassium sorbate according to mass ratio 1:1 composition.
The preparation process of seaweed extracted liquor, follows the steps below：
1) seaweed fragment, is prepared：Fresh Laminaria Japonica after cleaning is crushed, obtains seaweed fragment；
2) enzymolysis seaweed, is prepared：Seaweed fragment and water are fitted into agitator tank, sterilize 15min under the conditions of 100 DEG C, 30 DEG C of access enzymes are cooled to, digest 40min, the mass ratio 20 of seaweed fragment, water and enzyme:79.94:0.06, seaweed must be digested, The enzyme is lysozyme and pectase according to mass ratio 5:1 composition；
3) seaweed filtrate and seaweed slag, are prepared：The press filtration of enzymolysis seaweed is separated, gained filtrate is seaweed filtrate, and gained is residual Slag is seaweed slag；
4) fermentation of seaweed thing, is prepared：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to Mass ratio 95.5:1:0.5:1:1:1 is added in fermentation tank and mixes, and is adjusted to water content as 60% with water, goes out under the conditions of 120 DEG C Bacterium 20min, is cooled to 30 DEG C, obtains mixed culture medium, accesses rhodotorula mucilaginosa, and the mass ratio of rhodotorula mucilaginosa and mixed culture medium is 0.5:99.5, it is 30 DEG C in temperature, oxygen concentration is 12% in fermentation tank, and ferment 120h, is sterilized under the conditions of being 120 DEG C in temperature 20min, is cooled to 35 DEG C, obtains fermentation of seaweed thing；
5) seaweed extracted liquor and seaweed residue, are prepared：The seaweed filtrate that step 3) obtains is added to what step 4) obtained Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method The mass ratio of corynebacterium ammoniagenes is 98:2, it is 28 DEG C in temperature, under the conditions of oxygen content is 3mg/L, ferments 5 days, press filtration, gained filter Liquid is seaweed extracted liquor；Filter residue is seaweed residue；
The preparation of wherein thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for Strain；
B) thawing method improves the permeability of cell：In triplicate, its step is thawing method：The expansion that step 1) is obtained is again Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved, be placed again into -20 DEG C of refrigerator at room temperature, is freezed 4h, takes out, dissolves, be placed again into -20 DEG C of refrigerator at room temperature, freezes 4h, takes out, dissolves at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone 10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Wherein lysozyme follows the steps below：
(A) add the water of 5 times of weight to egg white or whole egg liquid, added after stirring evenly in homogenizer, homogeneous 3 times, homogeneous temperature Degree is 40 DEG C, and the homogeneous time is 15min, and wherein first time homogeneous pressure is 25MPa, and second of homogeneous pressure is 4MPa, Third time homogeneous pressure is 4MPa, is filtered after homogeneous, gained filtrate is dilution；
(B) the ultrafiltration membrane ultrafiltration for being 28000Da with molecular cut off by the dilution that step (A) obtains, obtain thin liquid and Dope；
(C) add resin in the thin liquid that step (B) obtains to be adsorbed, the part by weight of the thin liquid and resin is 1: 5, the resin after being adsorbed；
(D) resin after the absorption for obtaining step (C) is eluted with the NaCl aqueous solutions of 0.003mol/L, is washed De- liquid；
(E) by the eluent that step (D) obtains ultrafiltration membrane ultrafiltration desalination, ultrafiltrate, the retention of the ultrafiltration membrane are obtained Molecular weight is 8000Da；
(F) after the ultrafiltrate drying obtained step (E), that is, lysozyme is obtained.
Embodiment six
Plant hair conditioner by seaweed extracted liquor, surfactant, moisturizer, nourishing agent, thickener, pearling agent, essence, kill Microbial inoculum and water are according to mass ratio 35:5:8:10:0.5:3:0.5:0.5:37.5 compositions.The surfactant is polyglycereol fat Acid esters, lauric monoglyceride and cetyl trimethyl quaternary ammonium ammonium bromide are according to mass ratio 3:2:1 composition；The moisturizing Agent is glycerine, fructose, propane diols, sorbierite and polyglutamic acid according to mass ratio 1:1:2:1:1 composition；The nourishing agent It is honey, superoxide dismutase, Alphalin, vitamin e and vitamin E according to mass ratio 1:3:2:1:1 composition；Institute It is sodium alginate and sodium chloride according to mass ratio 1 to state thickener:2 composition；The pearling agent be the double stearic acid of ethylene glycol and Stearic acid diethylene glycol dilaurate is according to mass ratio 1:2 composition；The fungicide be methylisothiazolinone and potassium sorbate by According to mass ratio 1:1 composition.
1) seaweed fragment, is prepared：Fresh Laminaria Japonica after cleaning is dried, dry kelp is crushed using pulverizer, is obtained Seaweed fragment；
2) enzymolysis seaweed, is prepared：Seaweed fragment and water are fitted into agitator tank, sterilize 15min under the conditions of 100 DEG C, 30 DEG C of access enzymes are cooled to, digest 40min, the mass ratio 20 of seaweed fragment, water and enzyme:79.94:0.06, seaweed must be digested, The enzyme is lysozyme and pectase according to mass ratio 5:1 composition；
3) seaweed filtrate and seaweed slag, are prepared：The press filtration of enzymolysis seaweed is separated, gained filtrate is seaweed filtrate, and gained is residual Slag is seaweed slag；
4) fermentation of seaweed thing, is prepared：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to Mass ratio 95.5:1:0.5:1:1:1 is added in fermentation tank and mixes, and is adjusted to water content as 60% with water, goes out under the conditions of 120 DEG C Bacterium 20min, is cooled to 30 DEG C, obtains mixed culture medium, accesses rhodotorula mucilaginosa, and the mass ratio of rhodotorula mucilaginosa and mixed culture medium is 0.5:99.5, it is 30 DEG C in temperature, oxygen concentration is 12% in fermentation tank, and ferment 120h, is sterilized under the conditions of being 120 DEG C in temperature 20min, is cooled to 35 DEG C, obtains fermentation of seaweed thing；
5) seaweed extracted liquor and seaweed residue, are prepared：The seaweed filtrate that step 3) obtains is added to what step 4) obtained Stirred and evenly mixed in fermentation of seaweed thing, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, prepared by mixture and thawing method The mass ratio of corynebacterium ammoniagenes is 98:2, it is 28 DEG C in temperature, under the conditions of oxygen content is 3mg/L, ferments 5 days, press filtration, gained filter Liquid is seaweed extracted liquor；Filter residue is seaweed residue；
The preparation of wherein thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for Strain；
B) thawing method improves the permeability of cell：In triplicate, its step is thawing method：The expansion that step 1) is obtained is again Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved, be placed again into -20 DEG C of refrigerator at room temperature, is freezed 4h, takes out, dissolves, be placed again into -20 DEG C of refrigerator at room temperature, freezes 4h, takes out, dissolves at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone 10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams of immersions In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Wherein lysozyme follows the steps below：
(A) add the water of 5 times of weight to egg white or whole egg liquid, added after stirring evenly in homogenizer, homogeneous 2 times, homogeneous temperature Degree is 40 DEG C, and the homogeneous time is 15min, and wherein first time homogeneous pressure is 25MPa, and second of homogeneous pressure is 4MPa, Filtering after homogeneous, gained filtrate is dilution；
(B) the ultrafiltration membrane ultrafiltration for being 28000Da with molecular cut off by the dilution that step (A) obtains, obtain thin liquid and Dope；
(C) add resin in the thin liquid that step (B) obtains to be adsorbed, the part by weight of the thin liquid and resin is 1: 5, the resin after being adsorbed；
(D) resin after the absorption for obtaining step (C) is eluted with the NaCl aqueous solutions of 0.003mol/L, is washed De- liquid；
(E) by the eluent that step (D) obtains ultrafiltration membrane ultrafiltration desalination, ultrafiltrate, the retention of the ultrafiltration membrane are obtained Molecular weight is 8000Da；
(F) after the ultrafiltrate drying obtained step (E), that is, lysozyme is obtained.
Beneficial effects of the present invention are further illustrated with reference to experimental data：
One detection：
1st, material to be tested is tested
1 materials and methods：
1.1 test sites and experimental subjects：Yantai university chemistry analysis inspection center.
1.2 experiment detections：Survey the activity and the rate of recovery of lysozyme.
1.3 material to be tested：It is (even without homogenizer after the preparation method of lysozyme is except stirring evenly for common process Matter, other production methods are consistent with embodiment 1), embodiment 1, embodiment 2, embodiment 3, prepare in embodiment 4 and embodiment 5 Lysozyme do effect and compare.
1.4 experimental design：Using high performance liquid chromatography to sample treatment.
This experiment is consistent with other operations in addition to processing difference except testing.
2 results and analysis
Common process (homogeneous without homogenizer after stirring evenly, other production methods are consistent with embodiment 1), implement Bacteriolyze enzymatic treatment prepared by example 1, embodiment 2, embodiment 3, embodiment 4 and embodiment 5 is compared, and data are shown in Table 1
Table 1
The rate of recovery (%) Enzymatic activity (U/mg)
Common process 72.7 14567
Embodiment 1 91.7 21101
Embodiment 2 84.7 19831
Embodiment 3 88.3 20790
Embodiment 4 91.7 20903
Embodiment 5 89.3 20737
Compared by 1 embodiment 1 of table with lysozyme prepared by common process, the present invention either in the lysozyme rate of recovery, is gone back It is that enzymatic activity aspect is all significantly better than common process；The lysozyme prepared by embodiment 1 to embodiment 3 compares it can be seen that homogeneous Temperature still has certain influence to the rate of recovery and activity of lysozyme, and wherein embodiment 1 is best using 40 DEG C of general effects；By reality Apply example 1, lysozyme prepared by embodiment 4 and embodiment 5 compares it can be seen that homogeneous pressure and number are to the rate of recovery of lysozyme Also there is certain influence with activity, and non-pressure is bigger, the time is more long better, best results prepared by Integrated comparative embodiment 1.
Two application tests
1st, general information
Inventor selects 600 volunteers to use product of the present invention between in June, 2015~2017 year August, wherein man Property, each 300 of women, the range of age be 35~55 years old.Universal symptom is that hair tarnish is not smooth, hair quality well damage.Will This 600 experimenters are divided into 6 groups immediately, every group of 100 people, test respectively common shampoo (in addition to seaweed extracted liquor is not added, its Its production method is consistent with embodiment 1), contrast 1 (when preparing seaweed extracted liquor, use the production ammonia rod handled without thawing method Bacterium), 2 (except not enzyme treated in addition to, other production methods are consistent with embodiment 1) of contrast, contrast and 3 (remove lysozyme manufacturing process In stir evenly after without homogeneous processing outside, other production methods are consistent with embodiment 1), embodiment 1 and embodiment 6.
2nd, test method
During hair washing using experiment provide common surfactant (in addition to seaweed extracted liquor is not added, other production methods and reality It is consistent to apply example 1), contrast 1 (except prepare seaweed extracted liquor when, using the corynebacterium ammoniagenes handled without thawing method outside, other making Method is consistent with embodiment 1), 2 (except not enzyme treated in addition to, other production methods are consistent with embodiment 1) of contrast, contrast and 3 (remove After being stirred evenly in lysozyme manufacturing process without homogeneous processing outside, other production methods are consistent with embodiment 1), embodiment 1 With
Embodiment 6, uses 6 months.
3rd, judgment criteria and evaluation result
3.1 judgment criteria
3.2 evaluation results statistics such as table 1.
13 groups of surfactant Comparison of therapeutic of table
Group I (example) II (example) III (example) IV (example) Adverse reaction
Common shampoo 4 14 24 58 Nothing
Embodiment 1 35 28 26 11 Nothing
Embodiment 6 27 29 29 15 Nothing
Contrast 1 24 27 26 23 Nothing
Contrast 2 15 17 22 46 Have
Contrast 3 25 28 28 19 Nothing
It can be seen that the present invention is significantly better than common shampoo in terms of healthy hair and hair care is nourished by 1 data of table；Seaweed carries Take liquid to be prepared using Fresh Laminaria Japonica, nourishing healthy hair and hair care effect are significantly better than the effect of thing is dried using it；By embodiment 1 Embodiment 1 is can be seen that with 1 data of contrast (to remove significantly better than contrast 1 to nourishing healthy hair and hair care effect and prepare seaweed extracted liquor When, using the corynebacterium ammoniagenes handled without thawing method outside, other production methods are consistent with embodiment 1)；Embodiment 1, contrast 2 Enzyme treated, lysozyme effect using homogenizer homogeneous after better than not enzyme treated effect is relatively drawn with 3 data of contrast Fruit is better than normal agitation.

Claims (7)

  1. A kind of 1. preparation method of plant hair conditioner, it is characterised in that：
    It by seaweed extracted liquor, surfactant, moisturizer, nourishing agent, thickener, pearling agent, essence, fungicide and water according to Mass ratio 5~89:2~89:0~30:0~50:0.5~15:0~30:0~5:0~5:0~70 composition；
    The surfactant includes polyglyceryl fatty acid ester, lauric monoglyceride, methyl glycol fatty acid ester, sorbitol anhydride list Palmitate, stearoyl lactate, diacetyl tartarate monoglyceride, Cocoamidopropyl betaine, Nonyl pheno Ether, polyoxyethylene oleic acid ester, polyoxyethylene stearic acid ester, sorbitan fatty acid ester, two polyglycereol, cetyl trimethyl Quaternary ammonium ammonium bromide, octadecyl dimethyl benzyl quaternary ammonium ammonium chloride, benzyltriethylammoinium chloride, diisooctyl succinate sulfonic acid One or any ratio in sodium and aliphatic acid polyethenoxy fat it is two or more；
    The moisturizer is in glycerine, fructose, propane diols, sorbierite, polyglutamic acid, niacinamide, polyethylene glycol and xylitol One or any ratio it is two or more；
    The nourishing agent includes honey, sodium salicylate, superoxide dismutase, Alphalin, beta glucan, vitamin e, lysine One or any ratio in sodium and vitamin E it is two or more；
    The thickener includes one or any in sodium chloride, sodium carboxymethylcellulose, gelatin, sodium alginate and casein Ratio it is two or more；
    The pearling agent include one kind in pearl powder, stearic acid diethylene glycol dilaurate, ethylene glycol double stearic acid and mica powder or Arbitrary proportion it is two or more；
    The fungicide includes one kind in methylisothiazolinone, methylchloroisothiazandnone, sodium benzoate and potassium sorbate Or arbitrary proportion is two or more；
    The seaweed extracted liquor, follows the steps below：
    1）, prepare seaweed fragment：Seaweed after cleaning is crushed, obtains seaweed fragment；
    2）, prepare enzymolysis seaweed：Seaweed fragment and water are fitted into agitator tank, under the conditions of 60~120 DEG C sterilize 5~ 40min, is cooled to 27~45 DEG C of access enzymes, digests 30~60min, the mass ratio 5~30 of seaweed fragment, water and enzyme:70~ 95:0.02~1, seaweed must be digested；
    3）, prepare seaweed filtrate and seaweed slag：Enzymolysis seaweed is separated, gained filtrate is seaweed filtrate, and residue obtained is seaweed Slag；
    4）, prepare fermentation of seaweed thing：By seaweed slag, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to quality Than 95.5:1:0.5:1:1:1 is added in fermentation tank and mixes, and water content is adjusted to water as 50~70%, in 70~150 DEG C of conditions 5~40min of lower sterilizing, is cooled to 27~36 DEG C, obtains mixed culture medium, accesses rhodotorula mucilaginosa, rhodotorula mucilaginosa and mixed culture medium Mass ratio be 0.1~2:98~99.9, it is 27~36 DEG C in temperature, oxygen concentration is 8~21% in fermentation tank, fermentation 6~ 144h, sterilize 20~40min under the conditions of being 70~150 DEG C in temperature, is cooled to 20~40 DEG C, obtains fermentation of seaweed thing；
    5）, prepare seaweed extracted liquor and seaweed residue：By step 3）Obtained seaweed filtrate is added to step 4）Obtained seaweed Stirred and evenly mixed in fermentate, obtain mixture, access corynebacterium ammoniagenes prepared by thawing method, production ammonia prepared by mixture and thawing method The mass ratio of bar bacterium is 95~99.8:0.2~5, it is 25~35 DEG C in temperature, under the conditions of oxygen content is 1~8mg/L, fermentation 1 ~6 days, gained filtrate was seaweed extracted liquor after press filtration；Filter residue is seaweed residue；
    The seaweed is that one kind in Enteromorpha, kelp, sargassum, opotism and kelp or arbitrary proportion are two or more.
  2. 2. the preparation method of plant hair conditioner as claimed in claim 1, it is characterised in that：The preparation of thawing method corynebacterium ammoniagenes Follow the steps below：
    a）The expansion of strain is further cultured for：Using the thalline in exponential phase as seed, liquid is inoculated in 10% inoculum concentration In culture medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for strain；
    b）Thawing method improves the permeability of cell：Thawing method is at least repeated once, its step is：By step a）Obtained expansion is again Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved at room temperature；
    PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone 10.0g, NaCl 5.0g, agar 25.0g;
    The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping, weighs 550 grams and be soaked in In 1375ml water, soak a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
  3. 3. the preparation method of plant hair conditioner as claimed in claim 1, it is characterised in that：The enzyme is lysozyme and pectase According to mass ratio 5:1 composition, wherein lysozyme follow the steps below：
    (A) add the water of 5 times of weight to egg white or whole egg liquid, after stirring evenly add homogenizer in, at least it is homogeneous once, homogeneous temperature Spend for 30~60 DEG C, homogeneous pressure is 2~40MPa, and the homogeneous time is 10~40min, is filtered after homogeneous, and gained filtrate is dilution Liquid；
    (B) the dilution molecular cut off for obtaining step (A) is the ultrafiltration membrane ultrafiltration of 15000~30000Da, obtains thin liquid And dope；
    (C) add resin in the thin liquid that step (B) obtains to be adsorbed, the part by weight of the thin liquid and resin is 1:3~ 8, the resin after being adsorbed；
    (D) resin after the absorption for obtaining step (C) is eluted with the NaCl aqueous solutions of 0.001~0.5mol/L, is obtained Wash
    De- liquid；
    (E) by the eluent that step (D) obtains ultrafiltration membrane ultrafiltration desalination, ultrafiltrate, the retention molecule of the ultrafiltration membrane are obtained Measure as 2000~10000Da；
    (F) after the ultrafiltrate drying obtained step (E), that is, lysozyme is obtained；
    The resin is that the resin is cation exchange resin or macroporous absorbent resin；The model of cation exchange resin 001*7、732、AmberliteIR-120、Dowex-50、Lewatit-100、Diaion SK-1、AllassionCS、 One or more in Duolite C-20, SDB-3, macroporous absorbent resin AB-8, D101, D3520, X-5, NKA-II, One or more in NKA-9, S-8, XDA-1, H-20, H-30, H-40.
  4. 4. the preparation method of plant hair conditioner as claimed in claim 1, it is characterised in that：Step 3）The separation includes pressure It is more than one or both of filter, filtering and centrifugation.
  5. 5. the preparation method of plant hair conditioner as claimed in claim 1, it is characterised in that：Step 4）The mixed culture medium is extremely Water content is 60%, and the mass ratio of rhodotorula mucilaginosa and mixed culture medium is 0.5:99.5, it is 30 DEG C in temperature, oxygen concentration is 12%, ferment 120h, and sterilize 20min under the conditions of being 120 DEG C in temperature.
  6. 6. the preparation method of plant hair conditioner as claimed in claim 1, it is characterised in that：Step 5）Mixture and thawing legal system The mass ratio of standby corynebacterium ammoniagenes is 98:2, it is 28 DEG C in temperature, under the conditions of oxygen content is 3mg/L, ferments 5 days.
  7. 7. the preparation method of plant hair conditioner as claimed in claim 1, it is characterised in that：The rhodotorula mucilaginosa and production ammonia rod Bacterium is purchased from Chinese industrial Microbiological Culture Collection administrative center, and numbering is respectively CICC 31192 and CICC 10168.
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104146931A (en) * 2014-08-29 2014-11-19 皖南医学院 Natural nourishment and maintenance type hair conditioner and preparation method thereof
CN104958194A (en) * 2015-03-04 2015-10-07 上海应用技术学院 Hair conditioner containing flaxseed extract and preparation method thereof
CN107049830A (en) * 2017-05-04 2017-08-18 上海章华保健化妆品有限公司 A kind of high stability hair conditioner and preparation method thereof
CN107280994A (en) * 2017-05-04 2017-10-24 上海章华保健化妆品有限公司 One kind hydrolysis fibroin hair conditioner and preparation method thereof
CN107362074A (en) * 2017-09-14 2017-11-21 王雷 A kind of preparation method of algae hair conditioner

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104146931A (en) * 2014-08-29 2014-11-19 皖南医学院 Natural nourishment and maintenance type hair conditioner and preparation method thereof
CN104958194A (en) * 2015-03-04 2015-10-07 上海应用技术学院 Hair conditioner containing flaxseed extract and preparation method thereof
CN107049830A (en) * 2017-05-04 2017-08-18 上海章华保健化妆品有限公司 A kind of high stability hair conditioner and preparation method thereof
CN107280994A (en) * 2017-05-04 2017-10-24 上海章华保健化妆品有限公司 One kind hydrolysis fibroin hair conditioner and preparation method thereof
CN107362074A (en) * 2017-09-14 2017-11-21 王雷 A kind of preparation method of algae hair conditioner

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
周东坡，赵凯，马玺编著: "《生物制品学》", 31 January 2007, 化学工业出版社 *
段久芳编著: "《天然高分子材料》", 30 September 2016, 华中科技大学出版社 *

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Application publication date: 20180413