CN107794573A - 一种构建dna大片段文库的方法及其应用 - Google Patents
一种构建dna大片段文库的方法及其应用 Download PDFInfo
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- CN107794573A CN107794573A CN201611046464.1A CN201611046464A CN107794573A CN 107794573 A CN107794573 A CN 107794573A CN 201611046464 A CN201611046464 A CN 201611046464A CN 107794573 A CN107794573 A CN 107794573A
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Classifications
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
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- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Information Retrieval, Db Structures And Fs Structures Therefor (AREA)
Abstract
Description
目标片段大小 | ON/OFF时间(秒) | 循环数 |
300-400bp | 15/30 | 10 |
Claims (10)
Applications Claiming Priority (2)
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CN201610791466 | 2016-08-31 | ||
CN2016107914667 | 2016-08-31 |
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CN107794573A true CN107794573A (zh) | 2018-03-13 |
CN107794573B CN107794573B (zh) | 2022-09-13 |
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CN201611046464.1A Active CN107794573B (zh) | 2016-08-31 | 2016-11-23 | 一种构建dna大片段文库的方法及其应用 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111073952A (zh) * | 2018-12-29 | 2020-04-28 | 浙江安诺优达生物科技有限公司 | 构建dna文库的方法及其应用 |
CN114774411A (zh) * | 2022-06-16 | 2022-07-22 | 序康医疗科技(苏州)有限公司 | 大片段dna环化连接方法 |
Citations (7)
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CN102181943A (zh) * | 2011-03-02 | 2011-09-14 | 中山大学 | 一种配对双末端文库构建方法及用该文库进行基因组测序的方法 |
CN102534811A (zh) * | 2010-12-16 | 2012-07-04 | 深圳华大基因科技有限公司 | 一种dna文库及其制备方法、一种dna测序方法和装置 |
CN104153003A (zh) * | 2014-08-08 | 2014-11-19 | 上海美吉生物医药科技有限公司 | 一种基于illumina测序平台的大片段DNA文库的构建方法 |
CN104531874A (zh) * | 2014-12-29 | 2015-04-22 | 深圳华因康基因科技有限公司 | Egfr基因突变检测方法及试剂盒 |
CN104630206A (zh) * | 2015-02-05 | 2015-05-20 | 北京诺禾致源生物信息科技有限公司 | 转录组文库的构建方法 |
CN104711250A (zh) * | 2015-01-26 | 2015-06-17 | 北京百迈客生物科技有限公司 | 一种长片段核酸文库的构建方法 |
WO2016082130A1 (zh) * | 2014-11-26 | 2016-06-02 | 深圳华大基因研究院 | 一种核酸的双接头单链环状文库的构建方法和试剂 |
-
2016
- 2016-11-23 CN CN201611046464.1A patent/CN107794573B/zh active Active
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102534811A (zh) * | 2010-12-16 | 2012-07-04 | 深圳华大基因科技有限公司 | 一种dna文库及其制备方法、一种dna测序方法和装置 |
CN102534811B (zh) * | 2010-12-16 | 2013-11-20 | 深圳华大基因科技服务有限公司 | 一种dna文库及其制备方法、一种dna测序方法和装置 |
CN102181943A (zh) * | 2011-03-02 | 2011-09-14 | 中山大学 | 一种配对双末端文库构建方法及用该文库进行基因组测序的方法 |
CN104153003A (zh) * | 2014-08-08 | 2014-11-19 | 上海美吉生物医药科技有限公司 | 一种基于illumina测序平台的大片段DNA文库的构建方法 |
WO2016082130A1 (zh) * | 2014-11-26 | 2016-06-02 | 深圳华大基因研究院 | 一种核酸的双接头单链环状文库的构建方法和试剂 |
CN104531874A (zh) * | 2014-12-29 | 2015-04-22 | 深圳华因康基因科技有限公司 | Egfr基因突变检测方法及试剂盒 |
CN104711250A (zh) * | 2015-01-26 | 2015-06-17 | 北京百迈客生物科技有限公司 | 一种长片段核酸文库的构建方法 |
CN104630206A (zh) * | 2015-02-05 | 2015-05-20 | 北京诺禾致源生物信息科技有限公司 | 转录组文库的构建方法 |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111073952A (zh) * | 2018-12-29 | 2020-04-28 | 浙江安诺优达生物科技有限公司 | 构建dna文库的方法及其应用 |
CN111073952B (zh) * | 2018-12-29 | 2023-09-22 | 浙江安诺优达生物科技有限公司 | 构建dna文库的方法及其应用 |
CN114774411A (zh) * | 2022-06-16 | 2022-07-22 | 序康医疗科技(苏州)有限公司 | 大片段dna环化连接方法 |
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