CN107779426A - A kind of plant root edge cell coated Si processing method - Google Patents
A kind of plant root edge cell coated Si processing method Download PDFInfo
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- CN107779426A CN107779426A CN201711128461.7A CN201711128461A CN107779426A CN 107779426 A CN107779426 A CN 107779426A CN 201711128461 A CN201711128461 A CN 201711128461A CN 107779426 A CN107779426 A CN 107779426A
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- solution
- cacl
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- root edge
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
Abstract
The invention discloses a kind of plant root edge cell coated Si processing method, it is comprised the technical steps that：1) plant seedlings root border cell is collected in CaCl2Cell collection liquid is obtained in solution；2) cell collection liquid is moved to the CaCl of the polyethyleneimine containing 0.005mg/mL2In solution, 10min, CaCl are incubated2Solution cleans 1~2 time；3) step 2) resulting solution is transferred in silicate solution, is incubated 20min, CaCl is used after cell surface silication2Solution cleans.The present invention effectively forms nano-silicon clad by adjusting cell handling process on plant root edge cell surface, so as to provide technical foundation to improve plant root edge cell aluminum-resistant poison ability.
Description
Technical field
The present invention relates to plant cell process field, more particularly to a kind of plant root edge cell processing method.
Background technology
By the difference at removing toxic substances position, the physiological mechanism of plant aluminum-resistant poison is divided into outside repel and two major classes of internal tolerance.
The former detoxifies position in apoplast, and the latter is symplast.Repel mechanism including fixation of the cell membrane to aluminium, plasma membrane to aluminium in outside
Selection permeability, induction caused by barrier, sequestration ligand and it is phosphatic secretion, " gone out by active transport extracellular etc..It is and internal
Mechanism is resistant to mainly including organic acid, protein and other organic ligands in kytoplasm to the chelant of aluminium, the compartmentation of vacuole, aluminum-resistant
The induction of enzyme and the raising of enzymatic activity etc..In the past, the measure of aluminium poison, which is mitigated or eliminated, mainly includes liming, using calcium and magnesium phosphorus
Fertilizer and administration organic matter etc. improve soil to reduce the activity of aluminium in the soil liquid, and good environment is provided for plant growth, but
It is the aluminium poison that these measures can only solve the problems, such as table soil, the soil to heart bottom is difficult to play a role.Aluminium poison is widely regarded as
In acid soil (pH ＜ 5) an important factor for limiting plant growth.Result of study shows that root border cell is in protection plant root tip
It is important from having served during aluminium toxicity.
Cell induction SiO2The biomineralization process of nanostructure is self-assembly of, for many years always thing scholar and material
Expect chemist's problem very interested, it is for template with a small amount of organic macromolecule (protein, glycoprotein or polysaccharide), is carried out
Molecule manipulation, so as to be combined into the process of inorganic material with controlling the nucleation of mineral facies and growth and high-sequential.Current nanometer
Technology is being carried out in application agriculturally, and one of them is exactly to utilize to simulate biosynthesis strategy, develops new chemical preparation,
To understand and improve the anti-adversity ability of plant, and layer-by-layer (layer-by-layer self-as-sembly
Technique, LbL) it has been widely used in the association areas such as the surface modification of biology, but using just in biological husbantry
Starting, thus by electrostatic self-assembled technology apply with simulation biomineralization process will be very significant.
Many researchs show that silicon is used as plant beneficial element, can alleviate plant aluminum poison, and are deposited on cell membrane, carefully
Silica in intercellular space and cellular vessel, due to can induce SiO with the presence of various hydrophilic mediums2It is self-assembly of SiO2Receive
Rice structure, the effect of this deposition raising cell membrane resistance external force, but the SiO of nanostructured2How to be effectively deposited on
Root border cell, the more preferable aluminum-resistant poison ability of plant root edge cell is assigned, still needs to be probed into.
The content of the invention
It is an object of the invention to for above-mentioned the deficiencies in the prior art, there is provided at a kind of plant root edge cell coated Si
Reason method.
The technical solution used in the present invention is：A kind of plant root edge cell coated Si processing method, it includes as follows
Processing step：
1) plant seedlings root border cell is collected in CaCl2Cell collection liquid is obtained in solution；
2) cell collection liquid is moved to the CaCl of the polyethyleneimine containing 0.005mg/mL2In solution, 10min is incubated,
CaCl2Solution cleans 1~2 time；
3) step 2) resulting solution is transferred in silicate solution, is incubated 20min, CaCl is used after cell surface silication2It is molten
Liquid cleans.
As the further improvement of such scheme, the CaCl described in step 2)2Solution is the CaCl that concentration is 0.5mM/L2
Solution, its pH value are 7.0.
As the further improvement of such scheme, the silicate solution described in step 3) is by methyl silicate hydrating solution
With CaCl2Solution presses 50:The solution that 950 volume ratio mixes, its pH value are 7.0.
As the further improvement of such scheme, described methyl silicate hydrating solution is by 1.0M methyl silicate
The solution that the hydrochloric acid hydrolyzed in 1.0mM is formed.
As the further improvement of such scheme, the cell collection liquid described in step 1) is to take the tip of a root of 1cm plant seedlings
It is placed in the CaCl that concentration is 2mmol/L2Stirred in solution with glass bar obtained by 1~2min.
The beneficial effects of the invention are as follows：The present invention is by adjusting cell handling process, effectively in plant root edge cell
Surface forms nano-silicon clad, so as to provide technical foundation to improve plant root edge cell aluminum-resistant poison ability.
Brief description of the drawings
Fig. 1 is influence of the present invention to cytoactive；
Fig. 2 is the effect that rear cell processed by the invention alleviates aluminium poison under aluminium.
Embodiment
The present invention is specifically described with reference to embodiment, in order to art personnel to the present invention
Understand.It is necessary that herein the present invention will be further described it is emphasized that embodiment is only intended to, it is impossible to be interpreted as to this
The limitation of invention protection domain, art person skilled in the art, the non-intrinsically safe made according to foregoing invention content to the present invention
The modifications and adaptations of property, should still fall within protection scope of the present invention.Simultaneously following mentioned raw materials are unspecified, are
Commercially available prod；The processing step or preparation method not referred in detail be processing step known to a person skilled in the art or
Preparation method.
A kind of plant root edge cell coated Si processing method, it is comprised the technical steps that：
1) plant seedlings root border cell is collected in CaCl2Cell collection liquid is obtained in solution；
2) cell collection liquid is moved to the CaCl of the polyethyleneimine containing 0.005mg/mL2In solution, 10min is incubated,
CaCl2Solution cleans 1~2 time；
3) step 2) resulting solution is transferred in silicate solution, is incubated 20min, CaCl is used after cell surface silication2It is molten
Liquid cleans.
As the further improvement of such scheme, the CaCl described in step 2)2Solution is the CaCl that concentration is 0.5mM/L2
Solution, its pH value are 7.0.
Preferred embodiment is further used as, the silicate solution described in step 3) is by methyl silicate hydrating solution
With CaCl2Solution presses 50:The solution that 950 volume ratio mixes, its pH value are 7.0.
Preferred embodiment is further used as, described methyl silicate hydrating solution is by 1.0M methyl silicate
The solution that the hydrochloric acid hydrolyzed in 1.0mM is formed.
Preferred embodiment is further used as, the cell collection liquid described in step 1) is to take the tip of a root of 1cm plant seedlings
It is placed in the CaCl that concentration is 2mmol/L2Stirred in solution with glass bar obtained by 1~2min.
Embodiment
A kind of plant root edge cell coated Si processing method, it is comprised the technical steps that：
1) tip of a root of 1cm plant seedlings is taken to be placed in the CaCl that concentration is 2mmol/L2In solution with glass bar stirring 1~
2min, obtain cell collection liquid；
2) cell collection liquid is moved to the CaCl of the polyethyleneimine containing 0.005mg/mL2Solution (CaCl2Solution concentration
For 0.5mM/L, its pH value is in 7.0), is incubated 10min, CaCl2Solution cleans 2 times；
3) step 2) resulting solution is transferred to silicate solution (methyl silicate hydrating solution and CaCl2Solution presses 50:
The solution that 950 volume ratio mixes, its pH value is in 7.0), is incubated 20min, CaCl is used after cell surface silication2Solution
Cleaning.
Above-described embodiment processing gained cell collection liquid is subjected to cytoactive and alleviates the dependence test of aluminium poison ability, its
As shown in Figures 1 and 2, wherein CK is that nothing does any processing to test result；PE-coated represents to form self-assembled multilayer film
Cladding handles but without the cell of absorption silicon, and Si-coated, which represents to be formed after self-assembled multilayer film cladding is handled, carries out nano-silica
The cell of SiClx adsorption treatment, it can be obtained by the data of accompanying drawing 1 and accompanying drawing 2, it was demonstrated that processing method of the invention is not influenceing cell
The harmful effect of aluminium poison is effectively alleviated on the premise of activity.
Above-described embodiment is the preferred embodiments of the present invention, all with similar technique of the invention and the equivalence changes made,
The protection category of the present invention all should be belonged to.
Claims (5)
1. a kind of plant root edge cell coated Si processing method, it is characterised in that comprise the technical steps that：
1) plant seedlings root border cell is collected in CaCl2Cell collection liquid is obtained in solution；
2) cell collection liquid is moved to the CaCl of the polyethyleneimine containing 0.005mg/mL2In solution, 10min, CaCl are incubated2
Solution cleans 1~2 time；
3) step 2) resulting solution is transferred in silicate solution, is incubated 20min, CaCl is used after cell surface silication2Solution is clear
Wash.
A kind of 2. plant root edge cell coated Si processing method according to claim 1, it is characterised in that：In step 2)
Described CaCl2Solution is the CaCl that concentration is 0.5mM/L2Solution, its pH value are 7.0.
A kind of 3. plant root edge cell coated Si processing method according to claim 1, it is characterised in that：In step 3)
Described silicate solution is by methyl silicate hydrating solution and CaCl2Solution presses 50:950 volume ratio mixes molten
Liquid, its pH value are 7.0.
A kind of 4. plant root edge cell coated Si processing method according to claim 3, it is characterised in that：It is described just
Methyl silicate hydrating solution is that 1.0M methyl silicate is hydrolyzed into the solution that the hydrochloric acid in 1.0mM formed.
A kind of 5. plant root edge cell coated Si processing method according to claim 1, it is characterised in that：Step 1) institute
The cell collection liquid stated is that the tip of a root for taking 1cm plant seedlings is placed in the CaCl that concentration is 2mmol/L2Stirred in solution with glass bar
Obtained by 1~2min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711128461.7A CN107779426A (en) | 2017-11-15 | 2017-11-15 | A kind of plant root edge cell coated Si processing method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711128461.7A CN107779426A (en) | 2017-11-15 | 2017-11-15 | A kind of plant root edge cell coated Si processing method |
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| CN107779426A true CN107779426A (en) | 2018-03-09 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109729963A (en) * | 2019-02-01 | 2019-05-10 | 佛山科学技术学院 | A kind of nanometer siliceous deposits barrier heavy metal method based on rice material |
| CN109828005A (en) * | 2019-01-08 | 2019-05-31 | 中国科学院南京土壤研究所 | A method of identifying the resistance to aluminium toxicity of rice varieties can |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1907029A (en) * | 2006-08-09 | 2007-02-07 | 广东省生态环境与土壤研究所 | Rare earth composite silicasol capable of repressing rice absorbing high-density metal |
-
2017
- 2017-11-15 CN CN201711128461.7A patent/CN107779426A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1907029A (en) * | 2006-08-09 | 2007-02-07 | 广东省生态环境与土壤研究所 | Rare earth composite silicasol capable of repressing rice absorbing high-density metal |
Non-Patent Citations (5)
| Title |
|---|
| JIE MA等: "Multifunctionality of Silicified Nanoshells at Cell Interfaces of Oryza sativa", 《ACS SUSTAINABLE CHEMISTRY & ENGINEERING》 * |
| JUNO LEE等: "Cytoprotective Silica Coating of Individual Mammalian Cells through Bioinspired Silicification", 《ANGEW.CHEM.INT.ED》 * |
| MIN YU等: "The role of root border cells in aluminum resistance of pea (Pisum sativum) grown in mist culture", 《JOURNAL OF PLANT NUTRITION AND SOIL SCIENCE》 * |
| YINGMING FENG等: "Extracellular Silica Nanocoat Formed by Layer-By-Layer (LBL) Self-Assembly Confers Aluminum Resistance in Root Border Cells of Pea (Pisum Sativum)", 《J NANOBIOTECHNOLOGY》 * |
| 罗虹等: "硅、钙对水土保持植物荞麦铝毒的缓解效应", 《水土保持学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109828005A (en) * | 2019-01-08 | 2019-05-31 | 中国科学院南京土壤研究所 | A method of identifying the resistance to aluminium toxicity of rice varieties can |
| CN109729963A (en) * | 2019-02-01 | 2019-05-10 | 佛山科学技术学院 | A kind of nanometer siliceous deposits barrier heavy metal method based on rice material |
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Application publication date: 20180309 |