CN107743958A - A kind of manufacture craft of the fresh-keeping reagent of the Cut Carnation Flower of garlic extract - Google Patents
A kind of manufacture craft of the fresh-keeping reagent of the Cut Carnation Flower of garlic extract Download PDFInfo
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- CN107743958A CN107743958A CN201711246062.0A CN201711246062A CN107743958A CN 107743958 A CN107743958 A CN 107743958A CN 201711246062 A CN201711246062 A CN 201711246062A CN 107743958 A CN107743958 A CN 107743958A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
- A01N3/02—Keeping cut flowers fresh chemically
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Abstract
The invention discloses a kind of manufacture craft of the fresh-keeping reagent of the Cut Carnation Flower of garlic extract,Including the preparation of garlic water extract,Take the crushing sample 20g of garlic,Add 1000ml water,It is put into after soaking 12h in 50 DEG C of tepidariums and boils 30min again,Filter and remove residue,1000ml volumetric flask constant volumes,Obtain plant leaching liquor immersion stoste,4 DEG C of preservations of cooling,Concentration is 0.02g/l,Garlic extraneous liquid difference gradient is taken to be respectively placed in plug bottle,Wherein water extract takes 120ml,Clear water is separately added into plug bottle and is made into the liquid for immersing in bottle that total measurement (volume) is 300ml,I.e. water extract account for that clear water is made into total measurement (volume) 40%,The garlic Aqueous extracts 40% of the fresh-keeping reagent of Cut Carnation Flower,The Antistaling agent fresh-keeping effect of carnation is strong,The garlic water extract of the fresh-keeping reagent of Cut Carnation Flower accounts for clear water is made into total measurement (volume) 40%,The growth of microorganism can be suppressed,Reduce blocking of the microorganism to scape vascular tissue,Be advantageous to the water suction of cut-flower,And then beneficial to the fresh-keeping of cut-flower.
Description
Technical field
The present invention relates to a kind of cutflower preservation reagent, and in particular to a kind of fresh-keeping reagent of the Cut Carnation Flower of garlic extract
Manufacture craft.
Background technology
Carnation is carnation, also known as chimera China pink, carnation, great Hua China pinks, carnation, is Caryophyllaceae, China pink
Platymiscium, the ground such as Fujian, the Hubei in European temperate zone and China's Mainland are typically distributed about, originate in Mediterranean Region, are the world
One of the upper most common flowers of application [2].Carnation includes many mutation and cenospecies, almost can be successive in greenhouse
Bloom.From 1907, start the symbol using pink carnation as the Mother's Day, therefore the present is often by as the flower for dedicating mother to.It is fragrant
China pink is perennial herb.Because of petal Ju Tassel edge and fragrant strongly fragrant smell, and extensively cultivated.It is generally divided into flower bed carnation and flower
Shop carnation；Stem is grown thickly, and quality is hard, and in celadon, section expands, and height is about 50 ㎝.Floral leaf is thicker, in linear, to life.
Cauline leaf is similar to China pink, but more sturdy, is there is white powder.Flower is larger, has an armaticity smell, Dan Sheng, 2-3 fasciations or into
Cyme；There is four pieces of the avette squamella of water chestnut shape under calyx, tip is short and sharp, is about a quarter of calyx tube；Calyx tube is green
Color, five split；Petal is irregular, and edge is with teeth, single-lobe or polyphyll, have the patterns such as red, pink colour, yellow.April at florescence to September,
Protectorate cultivation perpetual bloom, Fresh Cutting flower belong to extremely tender and lovely, perishable product, and Fresh Cutting flower circulates to pass through after adopting and harvests, be whole
The links such as reason, classification, packaging and transport.The proportion of goods damageds in the rear process of circulation are adopted in China, and generally all more than 30%, remote way transport is damaged
Consumption rate is generally more than 40%.The reason for causing loss is a lot, and such as picking time is improper, and Postharvest handling is extensive, in transportation
Excessive dehydration, temperature is too high, and pernicious gas is accumulative etc..Loss Symptoms be flower can not open, petal too early wither,
Come off；Flower neck bending；Cauline leaf is withered and yellow；Blade dries up, come off；Flower fractures；Rot.Fully reinforced flowers enterprise is above-mentioned
It is synchronous with developed country in equipment, independent pre- freezer, cold room and freezer are such as set, be exclusively used in Fresh Cutting flower adopt rear precooling,
After classification cool down and transport before in, Long-term Cold Storage.And the medium-sized and small enterprises and peasant household based on domestic are generally not equipped with completely
Postharvest handling equipment, pretreatment and the fresh-keeping ability of transportation and sales after shortage is adopted, it is more serious that Fresh Cutting flower adopts rear loss ratio, domestic at present
Outside, the antistaling agent typically used in family is fresh-keeping is all liquid for immersing in bottle.Liquid for immersing in bottle is and of the same race because the difference of flower material has many kinds
Colored liquid for immersing in bottle compound method is also not quite similar.But included based on water and sugar.In addition to water and sugar, other guarantors
Fresh dose is generally placed in chemical bactericide, for suppressing the bacterial reproduction inside liquid for immersing in bottle, reduces it and cut-flower otch cell is broken
It is bad, it is normally carried out plant water absorbing capacity.Silver ion is added in the fresh-keeping reagent having, its fresh-keeping effect to Fresh Cutting flower is very
It is good, it is that fresh keeping time is most long in current antistaling agent.But the chemical reagent and heavy metal silver added is all a little harmful reagents, they
The life and health of people may be influenceed, while is also unfavorable for the protection of environment, while the configuration of existing antistaling agent is numerous and diverse, it is unfavorable
Operated in family, and plant easily makes cut-flower dehydration wither as the excessive concentration of cutflower preservation extract solution；And concentration is too low
Due sterilization fresh-keeping effect can not be played, so, the plant extraction liquid of debita spissitudo should be selected just to be advantageous to cutflower preservation.
The content of the invention
It is an object of the invention to provide a kind of manufacture craft side of the fresh-keeping reagent of the Cut Carnation Flower of garlic extract, with
Solve the protection for being unfavorable for environment, while the configuration of existing antistaling agent is numerous and diverse, is unfavorable for family's operation, and plant protects as cut-flower
The excessive concentration of fresh extract solution easily makes cut-flower dehydration wither；And concentration is too low can not play due sterilization fresh-keeping effect
Problem.
In order to solve the above-mentioned technical problem, the invention provides following technical scheme：
The invention provides a kind of manufacture craft side of the fresh-keeping reagent of the Cut Carnation Flower of garlic extract, preservation method is such as
Under：
Step 1：Carnation pre-processes, and carnation is put into water, then taken out successively, by wanting for the long 50cm of every plant of spray
Ask, cut the part for being longer than stem 50cm rapidly with oblique 45 degree of direction with blade, it is standby after having handled；
Step 2：Garlic pre-processes, and garlic is cleaned, and peels off crust, is positioned at shady and cool ventilation and air-dries 24 hours or so, then
It is put into baking oven and is dried 12 hours with 40 DEG C.Then smashed with mortar, obtaining garlic after 20 mesh sieves sieve crushes sample
Product, it is standby after having handled；
Step 3：Water extract extracts, and takes the crushing sample 20g of garlic, adds 1000ml water, is put into 50 DEG C of tepidariums and soaks
30min is boiled after bubble 12h again, filter and remove residue, 1000ml volumetric flask constant volumes, obtains plant leaching liquor immersion stoste, cools down 4 DEG C of preservations,
Concentration is 0.02g/l；
Step 4：Water extract enters bottle, takes garlic extraneous liquid difference gradient to be respectively placed in plug bottle, wherein water extract takes
120ml, clear water is separately added into plug bottle and is made into the liquid for immersing in bottle that total measurement (volume) is 300ml, be i.e. water extract accounts for clear water and is made into total measurement (volume)
40%；
Step 5：Cut Carnation Flower enters bottle, the carnation that 3 processes are handled well is inserted in plug bottle, during flower arrangement
Notice that lower clip there can not be bubble, and suitably to ensure the distance between each plug bottle, it is impossible to allow it mutually to extrude, between every flower
Scatter, be positioned in the room that room temperature is 21 DEG C~30 DEG C into hexagonal angle, and will not be by direct sunlight.
As a preferred technical solution of the present invention, the instrument that the preparation technology uses includes：The preparation technology
The instrument used includes：Plug bottle, triangular flask, measuring cup, beaker, glass bar, funnel and volumetric flask etc..
As a preferred technical solution of the present invention, the experiment flower material carnation takes on a red color, and there is one, petal on top,
Petal and the equal no disease and pests harm situation of blade, and character is substantially neat.
As a preferred technical solution of the present invention, garlic used in the garlic extract is edible garlic.
The beneficial effect that is reached of the present invention is：The invention is a kind of fresh-keeping reagent of the Cut Carnation Flower of garlic extract
Manufacture craft side, garlic water extract account for clear water be made into total measurement (volume) 40% antistaling agent pH value after the reduction of a period of time
Gradually tend towards stability, the growth of microorganism can be suppressed, reduce blocking of the microorganism to scape vascular tissue, be advantageous to cut-flower
Water suction, it is and then fresh-keeping beneficial to cut-flower, account for clear water with the presence of chemical ion, garlic water extract in garlic extraneous liquid and be made into always
The conductivity variations trend comparison of 40% antistaling agent of volume is gentle, and ascensional range is smaller, to the destructiveness of cell membrane compared with
Small, garlic water extract, which accounts for clear water and is made into 40% antistaling agent of total measurement (volume), plays certain bactericide and nutrient solution effect, greatly
Garlic water extract account for clear water be made into total measurement (volume) 40% antistaling agent salinity it is smaller, there is good preservativity, garlic water
Leaching liquor account for clear water be made into total measurement (volume) 40% antistaling agent implants POD activity it is strong, be advantageous to extend the life-span of cut-flower,
Be advantageous to cutflower preservation, the fresh-keeping effect of 40% concentration of garlic water extract and good while configuration are simple, are easy to family to operate.
In addition to objects, features and advantages described above, the present invention also has other objects, features and advantages.
Below by embodiment, the present invention is further detailed explanation.
Embodiment
Embodiment 1
The present invention provides a kind of manufacture craft side of the fresh-keeping reagent of the Cut Carnation Flower of garlic extract, and test method is such as
Under：
Step 1：Carnation pre-processes, and carnation is put into water, then taken out successively, by wanting for the long 50cm of every plant of spray
Ask, cut the part for being longer than stem 50cm rapidly with oblique 45 degree of direction with blade, it is standby after having handled；
Step 2：Garlic pre-processes, and garlic is cleaned, and peels off crust, is positioned at shady and cool ventilation and air-dries 24 hours or so, then
It is put into baking oven and is dried 12 hours with 40 DEG C.Then smashed with mortar, obtaining garlic after 20 mesh sieves sieve crushes sample
Product, it is standby after having handled；
Step 3：Water extract extracts, and takes the crushing sample 20g of garlic, adds 1000ml water, is put into 50 DEG C of tepidariums and soaks
30min is boiled after bubble 12h again, filter and remove residue, 1000ml volumetric flask constant volumes, obtains plant leaching liquor immersion stoste, cools down 4 DEG C of preservations,
Concentration is 0.02g/l；
Step 4：Water extract enters bottle, takes garlic extraneous liquid difference gradient to be respectively placed in plug bottle, wherein water extract takes
4 gradients of 60ml, 120ml, 180ml, 240ml, it is 300ml's that clear water is then separately added into each plug bottle and is made into total measurement (volume)
Liquid for immersing in bottle；
Step 5：Cut Carnation Flower enters bottle, using clear water and the fresh solution of Ke Li as control, then inserts 3 in each plug bottle
For branch by the carnation handled well, clip can not have bubble under paying attention to during flower arrangement, and suitably ensure each plug bottle
Between distance, it is impossible to allow it mutually to extrude.Scattered between every flower into hexagonal angle, be positioned over the room that room temperature is 21 DEG C~30 DEG C
In, and will not be by direct sunlight.Each gradient and water and Ke Li it is fresh be 3 repetitions, be respectively labeled as a, b, c.Each plug bottle
Numbering with garlic Chinese Pin Yin initial capitalize and concentration numeral plus its repeating label form, such as 60ml/300ml floodings
First repeating label of liquid liquid for immersing in bottle is DS 20a.
Embodiment 2
The present invention provides a kind of manufacture craft side of the fresh-keeping reagent of the Cut Carnation Flower of garlic extract, and collocation method is such as
Under：
Step 1：Carnation pre-processes, and carnation is put into water, then taken out successively, by wanting for the long 50cm of every plant of spray
Ask, cut the part for being longer than stem 50cm rapidly with oblique 45 degree of direction with blade, it is standby after having handled；
Step 2：Garlic pre-processes, and garlic is cleaned, and peels off crust, is positioned at shady and cool ventilation and air-dries 24 hours or so, then
It is put into baking oven and is dried 12 hours with 40 DEG C.Then smashed with mortar, obtaining garlic after 20 mesh sieves sieve crushes sample
Product, it is standby after having handled；
Step 3：Alcohol extract extraction, using Soakage extraction method three times, the garlic samples of crushing are weighed for the first time
50g, 95% quantitative ethanol is added, standard is according to dipped more than the sample 4cm of sample volume, immersion, is put into shaking table and shakes
48h.Decompression filters, and obtains filtrate and residue.Appropriate 95% ethanol is added in the residue carried for the first time for the second time, is just submerged
Sample 1cm.Immersion, it is put between shaking table and shakes 24h.Decompression filters, and obtains filtrate and residue.Third time adds in above-mentioned residue
Appropriate 95% ethanol, just submerges sample, immersion, is put between shaking table and shakes 12h.Decompression filters, and obtains filtrate, residue discards.Close
And filtrate three times, it is concentrated under reduced pressure, bath temperature is 25 DEG C or so.Concentrate constant volume to 20ml scales is tried with 95% ethanol
Guan Zhong, mass concentration are 1g/ml (i.e. the material containing 1g dry quality samples in 1ml extracts), be put into after sealing 0 in refrigerator~
4 DEG C of preservations；
Step 4：Alcohol extract enters bottle, takes garlic alcohol extract difference gradient to be respectively placed in plug bottle, alcohol steep
Liquid takes 3 gradients of 2.5ml/l, 5.0ml/l and 7.5ml/l, is then separately added into clear water in each plug bottle and is made into total measurement (volume) and is
300ml liquid for immersing in bottle；
Step 5：Cut Carnation Flower enters bottle, using clear water and the fresh solution of Ke Li as control, then inserts 3 in each plug bottle
For branch by the carnation handled well, clip can not have bubble under paying attention to during flower arrangement, and suitably ensure each plug bottle
Between distance, it is impossible to allow it mutually to extrude.Scattered between every flower into hexagonal angle, be positioned over the room that room temperature is 21 DEG C~30 DEG C
In, and will not be by direct sunlight.Each gradient and water and Ke Li it is fresh be 3 repetitions, be respectively labeled as a, b, c.Each plug bottle
Numbering with garlic Chinese Pin Yin initial capitalize and concentration numeral plus its repeating label form, such as 2.5ml/l alcohol steeps
First repeating label of liquid liquid for immersing in bottle is DS 2.5a.
Integrated embodiment 1 and embodiment 2 carry out Cut Carnation Flower observation, observe once within every 2 days, main detection scape and flower
Piece growth, developmental state, according to every time observed by result carry out corresponding record.Bottled sustaining time with flower withered death or
Scape bending lodging, untill losing ornamental value；Wherein observation of steps includes：
The measure of dianthus caryophyllus vase liquid pH value：Every 2 days measure is once.
Concrete operation step is as follows：The probe pen of PH analyzers is stretched into liquid for immersing in bottle to just submerging, shown on screen to be shown
After showing data stabilization, reading is carried out according to it.Probe pen will be cleaned with clear water before reagent is dipped every time, and be put into maintenance
Submerged in liquid, to ensure the accuracy of data.
The measure of dianthus caryophyllus vase liquid electrical conductivity, salinity and TDS：
Electrical conductivity, salinity and TDS measure are carried out to fresh-keeping liquid with conductivity gauge, was surveyed once every 2 days.
Specific measuring process is as follows：First each bar circuit of instrument is connected, then switched on power, probe pen is inserted into tested numbering
In a liquid for immersing in bottle, then to open instrument switch, to prevent probe pen energization oxidation by air.Switch successively simultaneously by mode button
Read three desired values and recorded, it should be noted that probe pen not touch inwall and the bottom of bottle, in test in test
Keep probe pen to fix, often surveyed a gradient, probe pen has been put into after being cleaned up in clear water and could be measured next time.
The measure of the slotting Carnation Petal POD enzymatic activitys of bottle：
Every 2 days harvesting samples once, win each different gradients and repeat 3~5 relatively good petals on the slotting cut-flower of bottle
It is put into corresponding sack, it is labelled as mark, it is put into refrigerating box and is saved backup with 4 DEG C.Treat that conventional index determines
After, it is drawn off, weighs about 3g petals in mortar, adds liquid nitrogen, rapid grinding, 5mlpH=is added after grinding completely
7.8 PBS extract solutions mixing, is fitted into centrifuge tube after well mixed.Centrifugation in centrifuge is placed on to take out after 15 minutes, will
Centrifugal clear liquid is fitted into 4 DEG C of refrigeration in corresponding centrifuge tube, standby.
Specific measuring process is as follows：The measure of blade POD activity：POD reaction mixtures 3.0ml notes are first extracted with liquid-transfering gun
Enter in cuvette, then take out the centrifugate after refrigeration, take its supernatant liquor about 0.1ml to inject same cuvette with liquid-transfering gun
In, the measure that enzyme reaction speed is carried out in spectrophotometer is put into after being shaken up, each concentration is each repeated three times, and is remembered
It is averaged after record.Control is to be not added with enzyme liquid as with reference to (being replaced with Extraction buffer).
Form is compared in the carnation mode of appearance change of different disposal：
Form 1
The every 2 days record upright degree of scape make table as above with flowering degree.In carnation it can be seen from form 1
During cut-flowers in bottle, the effect of four water extract processing is better than better than control clear water, the effect of two processing in seven processing
Control can be sharp fresh, it was demonstrated that the water extract of middle garlic is fresh-keeping to Cut Carnation Flower to be acted on.When May 19, greatly
Part flower withers and falls, but water extract also has most of flower not wither and fall, and alcohol extract is when May 21 with regard to all withering
Fall.Total fresh-keeping effect water logging can be seen that by open hour for spending, the lodging situation of scape, petal time etc. of withering and falling
Extract ＞ controls can the fresh ＞ controls water ＞ alcohol extracts of profit.Further analyze various concentrations and water is found to the fresh-keeping effect of carnation
Leaching liquor (40%) effect is best, and water extract (60%) takes second place, and alcohol extract (5.0ml/l) effect is worst.Various extractions
The fresh-keeping effect of various concentrations is also different in liquid, and with the increase of concentration, fresh keeping time is shortening.
PH value reflects the acid-base value of solution, and peracidity and overbasic solution are all unfavorable for the water suction of cut-flower.Typically
Think, relatively low pH can suppress the growth of microorganism, reduce blocking of the microorganism to scape vascular tissue, be advantageous to cut-flower
Water suction, it is and then fresh-keeping beneficial to cut-flower.Drawn and found out by experiment, the pH value of various processing is gradual after the reduction of a period of time
Tend towards stability.The fresh-keeping liquid pH value of four gradients of water extract changes between 3 to 5, is minimum, guarantor among several processing
Fresh effect is best.The fresh-keeping liquid of alcohol extract is ranged up in relatively unstable of extension its pH value over time
7.0, minimum 4.0 or so, fresh-keeping effect is worst.
The size of electrical conductivity reflects the size of liquid for immersing in bottle intermediate ion concentration, and the size of ion concentration more indirectly illustrates
The destructiveness of cell membrane.Due to consideration that contained ion concentration is different in itself for the fresh-keeping liquid of different disposal, therefore I
Compare the dynamic change of electrical conductivity.Figure is drawn with the extension of time, the electrical conductivity of various liquid for immersing in bottle is all rising by testing
Height, show that extension cell membrane disruption degree over time is gradually increasing, but ascensional range is relative and little.Further
Found after being compared to concentration, electrical conductivity is directly proportional with concentration.This explanation, with the presence of chemical ion in garlic extraneous liquid, because
And the practical significance for comparing electrical conductivity merely will be lowered.Therefore we analyze the size of electrical conductivity rising, approximately weigh
The degree that is damaged of cell membrane.Due to the difference on the collocation method of two kinds of leaching liquors, cause their basic conductivity and electricity
Conductance climbing speed will have a different changes, thus we the effect of two kinds of different leaching liquors will be carried out splitting ratio compared with.It is logical
More several different processing are crossed we have found that the variation tendency of 60% leaching liquor is than shallower in water extract, ascensional range
It is smaller, followed by the leaching liquor of 40% concentration；And in alcohol extract change curve than it is shallower be that concentration is 2.5ml/l
Alcohol extract, the amplitude of rising is very small.Illustrate it is best with 60% leaching liquor effect in the various concentrations of water extract,
40% takes second place；It is and best with 2.5ml/l effect in alcohol extract.
TDS (Total Dissolved Solids, total dissolved solid) is that the important synthesis of a measure reagent cleanliness refers to
Mark.TDS values are bigger, illustrate that solution concentration is bigger.It can be considered as approximately combine electrical conductivity, micro organism quantity and
The comprehensive numerical value of one of solution impurity.Show that the various concentrations of two kinds of liquid for immersing in bottle and the TDS of control have rising by experiment
Trend, this can illustrate the gradual extension with fresh keeping time, and electrical conductivity, micro organism quantity and the solution impurity of liquid for immersing in bottle all exist
Rise.By experiment, we can draw, the tendency of two kinds of leaching liquors has obvious difference.In the various gradients of water extract
In, the ascensional range of 60% concentration is minimum, followed by 40%, gap and unobvious.Illustrate that the water extract of garlic really can
Play certain bactericide and nutrient solution effect but effect and unobvious, concentration too Gao Zehui cause in the case that concentration is too low
Microorganism growth is too fast.Also the nutrient solution effect of side light garlic is more than bactericide and acted on for this.And in alcohol extract
It is minimum with the liquid for immersing in bottle ascensional range of 2.5ml/L concentration in various gradients, and its trend is similar to clear water control, illustrates ethanol
The effect of leaching liquor is not fine.
Many inorganic salts such as salt sylvite, calcium salt, aluminium salt, mantoquita, nickel salt and zinc salt can increase the osmotic potential and flower of solution
The turgescence of valve cell, suppress the activity of microorganism in the aqueous solution, be advantageous to the water tariff collection balance in spray, extend carnation bottle
Insert the life-span.But from experiment we have found that salinity is inversely proportional with fresh-keeping effect, salinity is bigger, and fresh-keeping effect is poorer.Tried from form
As can be seen that the salinity altercation degree of the processing of garlic Aqueous extracts 400% is minimum, so fresh-keeping effect is best in testing.
Important physical signs of the POD as plant tissue aging, its major function is to remove free radical, anti-oxidant, is delayed
Aging.For cutflower preservation, POD activity is stronger in its plant, is more advantageous to extend the life-span of cut-flower, is more advantageous to
Cutflower preservation.Show that in preservation process, the POD activity respectively handled is all in rising trend from us are tested.It is in particular to last
3 observation, ascendant trend are stronger.And the liquid for immersing in bottle that alcohol extract makes has to a certain degree in observation a few days ago
On fluctuation.From the point of view of experiment, enzymatic activity is fresh-keeping in there is the fluctuation status fallen to be obviously unfavorable for, and the water of four kinds of concentration
The variation tendency of leaching liquor illustrates POD activity kept in balance, is advantageous to cutflower preservation generally in plateau.As for
Since the 7th day, later change became to having rising by a relatively large margin.
Found by embodiment 1 and embodiment 2：Garlic extraneous liquid has significantly delayed the aging of carnation, extends perfume (or spice)
The Vase time of China pink.Variety classes, the garlic extraneous liquid of various concentrations are different to the fresh-keeping effect of carnation.Wherein with garlic
The fresh-keeping effect of 40% concentration of water extract is optimal.Although among six indexs being measured, the electricity of ethanol extract
The physical signs such as conductance, TDS and salinity perform better, but influence the effect of be largely because ethanol itself.Ethanol
The bactericidal effect of itself and stronger dissolubility cause its leaching liquor effect to be better than water extract among above index.By
Form observe and pH value measure etc. in terms of numerical value we it can be found that the liquid for immersing in bottle of water extract in this several influence of ethanol abilities
Have obvious advantage in terms of less index, wherein using concentration as 40% best results.Especially to the outer of Carnation Petal
In sight morphological analysis it can be found that in mode of appearance in this important physical signs, the Contrast on effect and ethanol of water extract
There is very big advantage in terms of leaching liquor.Secondly in terms of POD measure, peroxidase major function is to remove free radical, antioxygen
Change, anti-aging, and the POD activity of water extract is more stable, can be more beneficial for extending in the life-span of cut-flower, being advantageous to cut-flower
It is fresh-keeping.In summary several indexs, the garlic water extract of 40% concentration have outstanding in mode of appearance, POD activity etc.
Performance, fresh-keeping effect is obvious.The water extract for thereby determining that 40% concentration is the optimal anti-staling agent of this experiment.
The beneficial effect that is reached of the present invention is：The invention is a kind of fresh-keeping reagent of the Cut Carnation Flower of garlic extract
Manufacture craft side, garlic water extract account for clear water be made into total measurement (volume) 40% antistaling agent pH value after the reduction of a period of time
Gradually tend towards stability, the growth of microorganism can be suppressed, reduce blocking of the microorganism to scape vascular tissue, be advantageous to cut-flower
Water suction, it is and then fresh-keeping beneficial to cut-flower, account for clear water with the presence of chemical ion, garlic water extract in garlic extraneous liquid and be made into always
The conductivity variations trend comparison of 40% antistaling agent of volume is gentle, and ascensional range is smaller, to the destructiveness of cell membrane compared with
Small, garlic water extract, which accounts for clear water and is made into 40% antistaling agent of total measurement (volume), plays certain bactericide and nutrient solution effect, greatly
Garlic water extract account for clear water be made into total measurement (volume) 40% antistaling agent salinity it is smaller, there is good preservativity, garlic water
Leaching liquor account for clear water be made into total measurement (volume) 40% antistaling agent implants POD activity it is strong, be advantageous to extend the life-span of cut-flower,
Be advantageous to cutflower preservation, the fresh-keeping effect of 40% concentration of garlic water extract and good while configuration are simple, are easy to family to operate.
Finally it should be noted that：The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used
To be modified to the technical scheme described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic.
Within the spirit and principles of the invention, any modification, equivalent substitution and improvements made etc., it should be included in the present invention's
Within protection domain.
Claims (4)
1. a kind of manufacture craft of the fresh-keeping reagent of the Cut Carnation Flower of garlic extract, it is characterised in that preservation method is as follows：
Step 1：Carnation pre-processes, and carnation is put into water, then taken out successively, by the long 50cm of every plant of spray requirement, uses
Blade cuts rapidly the part for being longer than stem 50cm with oblique 45 degree of direction, standby after having handled；
Step 2：Garlic pre-processes, and garlic is cleaned, and peels off crust, is positioned at shady and cool ventilation and air-dries 24 hours or so, places into
Dried 12 hours with 40 DEG C in baking oven.Then smashed with mortar, garlic is obtained after 20 mesh sieves sieve and crushes sample, place
It is standby after having managed；
Step 3：Water extract extracts, and takes the crushing sample 20g of garlic, adds 1000ml water, is put into 50 DEG C of tepidariums and soaks
30min is boiled after 12h again, filter and remove residue, 1000ml volumetric flask constant volumes, obtains plant leaching liquor immersion stoste, cools down 4 DEG C of preservations, it is dense
Spend for 0.02g/l；
Step 4：Water extract enters bottle, takes garlic extraneous liquid difference gradient to be respectively placed in plug bottle, wherein water extract takes
120ml, clear water is separately added into plug bottle and is made into the liquid for immersing in bottle that total measurement (volume) is 300ml, be i.e. water extract accounts for clear water and is made into total measurement (volume)
40%；
Step 5：Cut Carnation Flower enters bottle, and the carnation that 3 processes are handled well is inserted in plug bottle, is paid attention to during flower arrangement
Lower clip can not have bubble, and suitably ensure the distance between each plug bottle, it is impossible to allow it mutually to extrude, between every flower into
Hexagonal angle scatters, and is positioned in the room that room temperature is 21 DEG C~30 DEG C, and will not be by direct sunlight.
2. a kind of manufacture craft side of the fresh-keeping reagent of Cut Carnation Flower of garlic extract according to claim 1, it is special
Sign is that the instrument that the preparation technology uses includes：Plug bottle, triangular flask, measuring cup, beaker, glass bar, funnel and volumetric flask
Deng.
3. a kind of manufacture craft side of the fresh-keeping reagent of Cut Carnation Flower of garlic extract according to claim 1, it is special
Sign is that the experiment flower material carnation takes on a red color, and there are one, petal, petal and the equal no disease and pests harm situation of blade, and property in top
Shape is substantially neat.
4. a kind of manufacture craft side of the fresh-keeping reagent of Cut Carnation Flower of garlic extract according to claim 1, it is special
Sign is that garlic used in the garlic extract is edible garlic.
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| CN201711246062.0A CN107743958A (en) | 2017-12-01 | 2017-12-01 | A kind of manufacture craft of the fresh-keeping reagent of the Cut Carnation Flower of garlic extract |
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| CN107743958A true CN107743958A (en) | 2018-03-02 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108669072A (en) * | 2018-03-27 | 2018-10-19 | 靖西市秀美边城农业科技有限公司 | A kind of antistaling agent for cut-flower and the preparation method and application thereof |
| CN113100229A (en) * | 2021-04-29 | 2021-07-13 | 昆明理工大学 | Application of chlorogenic acid and honeysuckle extract in delaying blooming of fresh cut flower buds |
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| WO2012051670A1 (en) * | 2010-10-21 | 2012-04-26 | Byron Food Science Pty Limited | Method for preserving plant material |
| CN103548819A (en) * | 2013-10-31 | 2014-02-05 | 浙江农林大学天目学院 | Flower preservative containing Chinese herbal medicine extract and application thereof |
| CN106135198A (en) * | 2016-08-01 | 2016-11-23 | 西南林业大学 | A kind of antistaling agent improving Carnation Flower Vase time and preparation method thereof |
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| WO2012051670A1 (en) * | 2010-10-21 | 2012-04-26 | Byron Food Science Pty Limited | Method for preserving plant material |
| CN103548819A (en) * | 2013-10-31 | 2014-02-05 | 浙江农林大学天目学院 | Flower preservative containing Chinese herbal medicine extract and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108669072A (en) * | 2018-03-27 | 2018-10-19 | 靖西市秀美边城农业科技有限公司 | A kind of antistaling agent for cut-flower and the preparation method and application thereof |
| CN113100229A (en) * | 2021-04-29 | 2021-07-13 | 昆明理工大学 | Application of chlorogenic acid and honeysuckle extract in delaying blooming of fresh cut flower buds |
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Application publication date: 20180302 |