CN106135198A - A kind of antistaling agent improving Carnation Flower Vase time and preparation method thereof - Google Patents
A kind of antistaling agent improving Carnation Flower Vase time and preparation method thereof Download PDFInfo
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- CN106135198A CN106135198A CN201610618735.XA CN201610618735A CN106135198A CN 106135198 A CN106135198 A CN 106135198A CN 201610618735 A CN201610618735 A CN 201610618735A CN 106135198 A CN106135198 A CN 106135198A
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- Prior art keywords
- radix
- caulis opuntiae
- opuntiae dillenii
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- residue
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
- A01N3/02—Keeping cut flowers fresh chemically
Abstract
The invention discloses a kind of antistaling agent improving Carnation Flower Vase time, be made up of following components according to mass percent: two kinds of lixiviating solution of Radix et Caulis Opuntiae Dillenii 12% 18%, Pericarpium Zanthoxyli 1% 5%, Radix Notoginseng 0.5% 0.8%, Fructus Capsici 2% 6%, Rhizoma Gastrodiae 7% 15%, chitin 3% 7%, the Radix Stemonae 0.5% 1.5%, Flos Caryophylli 0.5% 1.5%, Rhizoma Alpiniae Officinarum 0.5% 1.5%, kieselguhr 2% 6%, surplus are water.The present invention also provides for the preparation method of a kind of antistaling agent improving Carnation Flower Vase time, and the present invention i.e., for the purpose of extending the cut-flowers in bottle life-span, accomplishing ecological fresh-keeping research, has filtered out the Novel ring antistaling agent for cut-flower of cheaper, environmental protection.
Description
Technical field
The invention belongs to Garden Engineering field, specifically, relate to a kind of improving the fresh-keeping of Carnation Flower Vase time
Agent and preparation method thereof.
Background technology
Fresh Cutting flower is a kind of farm product with ornamental value, and its commodity value views and admires the close phase of quality with Fresh Cutting flower
Closing, it views and admires quality can pass through petal, the flower size of sheet, color and luster, and blade coloring, viewing and admiring the time etc. embodies.Fresh Cutting flower
After gathering, easily addle, loss rate about 20% after the dish of cut-flower.The fresh-keeping improper loss caused is up to 40%-50%, therefore profit
Extend the Fresh Cutting flower life-span with complex art, extend its turnover period and commodity sight is particularly significant.Restriction Fresh Cutting flower produce because of
Element is a lot, and wherein the fresh-keeping of cut-flower is key issue.
Dianthus carryophyllus is to apply one of most common flowers in the world at present, is one of most popular cut-flower, can be made for inserting
Flower, corsage etc..Dianthus carryophyllus is perennial root draft.Because of petal tool edge and fragrant strongly fragrant abnormal smells from the patient, and extensively cultivated.It is generally divided into flower
Altar Dianthus carryophyllus and florist's shop Dianthus carryophyllus two class；Dianthus carryophyllus breeding sowing, press strip and cuttage, and based on cuttage.Skewer
Insert in addition to the hot summer, the most all can carry out, especially best with late January to early Febuary cutting experiment.
Should be noted that Dianthus carryophyllus, in cut-flower postharvest handling, is vulnerable to Infected with Pathogenic Fungi and putrid and deteriorated, the annual world is each
The cut-flower that ground produces is at a relatively high to selling period loss after gathering, and the most all attaches great importance to the post-harvest fresh-keeping work of cut-flower.
Pathogen can be killed when utilizing chemical preservative to process, control the growth of latency pathogen, and can be to a certain extent
The physiological metabolism of suppression cut-flower, keeps the quality of cut-flower, extends shelf life.Along with the development of science and technology, people recognize chemical drugs
Agent has health risk, pollutes environment etc. defect.From natural resources, find active substance replace chemical substance, be subject in recent years
To extensive concern both domestic and external, the research and development of natural preservative seem particularly significant.
The fresh-keeping approach of Carnation Flower is a lot, mainly has cold storing and fresh-keeping, both at home and abroad about fresh-keeping the grinding of Carnation Flower
Study carefully report a lot, use technique of refrigeration, bagging moisturizing cold preservation at 4 DEG C, the at this moment fresh keeping time of Carnation Flower, vase life
The longest.Can only be inserted in water storage, transport team the short time, otherwise the alabastrum on inflorescence top is the most wilting.Adopt before cutting vanning, available
Silver thiosulfate solution dipping scape 20 minutes；Or the mixing of salt arabo-ascorbic acid early is played with sugar, 8-hydroxyquinoline citric acid
Liquid inserts guarantor's liquid to improve ornamental value and the economic benefit of Carnation Flower, Chinese scholars pair as the bottle of Carnation Flower
Its preservation method has carried out a large amount of in-depth study.But, great majority are about the reality of Carnation Flower Study on preservation technique at present
Test processing method the most single, be often confined to some aspect, between various dissimilar processing methods the most still
Having no report, this makes numerous achievement in research receive certain restriction during the production application of market.To this end, in recent years
Achievement in research about Carnation Flower preservation technique carries out the summary and review of system, it is intended to for Carnation Flower preservation technique
Market application and future development provide certain reference, and are looked forward to its developing prospect, it is believed that be suitable for widely
Property, the controlling of reliable safety and system be three great development directions of following preservation technique.Dianthus carryophyllus is excellent flower bed
With flower border material, become the principal item of business cut-flower cultivation.
Chinese medicine is fresh-keeping is just to have risen the preservation method of coming in recent years.Fresh-keeping some effective ingredient utilizing Chinese medicine exactly of Chinese medicine
Antibacterial or the bactericidal action being had, suppresses bottle to insert the growth and breeding of microorganism in solution, thus reduces blocking and absorb moisture
Conduit so that cut-flower can absorb water to greatest extent, extends the fresh keeping time of cut-flower.Edible Xiang Xin section plant the most all belongs to
In Chinese herbal medicine category.The antibacterial antisepsis of Xiang Xin section plant can trace back to the wood of three, 4,000 Nian Qian ancient Egypts still she.Nearly
Since oneth century, scientists has carried out bacteriostasis antibiosis test to multiple Xiang Xin section plant, find many Xiang Xin section plants and
Its quintessence oil all contains antimicrobial component, has antibacterial efficacy in various degree.Natural edible spice, also known as fumet, refers to containing each
Plant aroma volatile, a class natural materials of food flavor can be given.In addition to there is flavouring effect, they much possibly together with
Multiple nutritional components and medical value.Scientists with the conventional flavouring agent Rhizoma Zingiberis Recens of family, Bulbus Allii, Fructus Anisi Stellati, Fructus Piperis, Fructus Capsici,
The extracting solution of 13 kinds of natural perfume materials such as Cortex Cinnamomi, Pericarpium Zanthoxyli, Herba Menthae, Flos Caryophylli, Fructus Amomi, Fructus Foeniculi, Pericarpium Citri Reticulatae and Fructus Cumini Cymini to escherichia coli,
Bacillus proteus, Acetobacter pasteurianus, bacillus subtilis, staphylococcus aureus, streptococcus pneumoniae, shigella dysenteriae, mouse typhus
The several frequently seen microorganisms such as Salmonella, aspergillus niger, aspergillus oryzae, Aspergillus flavus have carried out extracorporeal bacteria inhibitor test, find theirs
Extracting solution all shows certain bacteriostasis to antibacterial, so have is used as fresh-keeping liquid.
The easy cooperating microorganisms of otch of cut-flower, affect membrane permeability and hinder the normal moisture of cut-flower and nutrition absorption and
Conduction, and containing sterilization or antibacterial substance in Chinese crude drug extracting solution, the growth and breeding of the microorganisms such as antibacterial can be suppressed, reduce it right
The destruction of cut-flower stem, simultaneously possibly together with saccharide homenergic material in extracting solution, can promote absorption and the conduction of moisture, thus prolong
Long cutting flower life, reaches fresh-keeping effect.
Summary of the invention
In view of this, the present invention is directed to above-mentioned problem, it is provided that a kind of improve the fresh-keeping of Carnation Flower Vase time
Agent and preparation method thereof, the present invention i.e., for the purpose of extending the cut-flowers in bottle life-span, accomplishing ecological fresh-keeping research, has filtered out phase
To inexpensive, the Novel ring antistaling agent for cut-flower of environmental protection.
In order to solve above-mentioned technical problem, the invention discloses a kind of antistaling agent improving Carnation Flower Vase time,
It is made up of following components according to mass percent: Radix et Caulis Opuntiae Dillenii lixiviating solution 12%-18%, Pericarpium Zanthoxyli 1%-5%, Radix Notoginseng 0.5%-
0.8%, Fructus Capsici 2%-6%, Rhizoma Gastrodiae 7%-15%, chitin 3%-7%, Radix Stemonae 0.5%-1.5%, Flos Caryophylli 0.5%-1.5%,
Rhizoma Alpiniae Officinarum 0.5%-1.5%, kieselguhr 2%-6%, surplus are water.
Further, Radix et Caulis Opuntiae Dillenii lixiviating solution includes Radix et Caulis Opuntiae Dillenii water extract and Radix et Caulis Opuntiae Dillenii alcohol extract.
Further, Radix et Caulis Opuntiae Dillenii water extract is prepared by the following method and obtains:
1) pretreatment of Radix et Caulis Opuntiae Dillenii: dry at Yin Liang, is adjusted to 35-40 DEG C by drying baker, dries 8-16 hour, will with powder beater
Material disintegrating, crosses 15-25 mesh sieve, packs respectively, and 4 DEG C save backup；
2) take the pulverizing sample of raw Radix et Caulis Opuntiae Dillenii, add water, put into and boil 20-again after 8-16h is soaked in 45-55 DEG C of tepidarium
40min, filter and remove residue, 1000ml volumetric flask constant volume, obtain plant lixiviating solution stock solution, cool down 4 DEG C and save backup, concentration is
0.02g/l。
Further, Radix et Caulis Opuntiae Dillenii alcohol extract prepares in the following manner:
1) pretreatment of Radix et Caulis Opuntiae Dillenii: dry at Yin Liang, is adjusted to 35-40 DEG C by drying baker, dries 8-16 hour, will with powder beater
Material disintegrating, crosses 15-25 mesh sieve, packs respectively, and 4 DEG C save backup；
2) taking the Radix et Caulis Opuntiae Dillenii plant sample of pulverizing, add the ethanol that quantitative volumn concentration is 95%, standard is root
According to dipped more than the sample 4cm of sample volume, soak, put into concussion 45-51h in shaking table；Decompression sucking filtration, obtains filtrate and residue；
3) adding volumn concentration in the residue carried for the first time is 95% ethanol, just submergence sample 1cm, leaching
Bubble, puts into concussion 20-28h between shaking table；Decompression sucking filtration, obtains filtrate and residue；
4) in above-mentioned residue, add 95% ethanol, just submergence sample, soak, put into concussion 8-16h between shaking table；Subtract
Pressure sucking filtration, obtains filtrate, and residue discards；
5) concentrate: merging three filtrates, carry out concentrating under reduced pressure, bath temperature is 20-30 DEG C；
6) constant volume: be 95% ethanol in concentrated solution constant volume to 20ml scale test tube with volumn concentration, mass concentration
For 1g/ml, i.e. 1ml extract contains the material of the dry plant sample of 1g, after sealing, put into 0～4 DEG C of preservation in refrigerator, standby.
Further, the addition of Radix et Caulis Opuntiae Dillenii water extract accounts for the 20%-40% of the volume content of total liquid for immersing in bottle, Radix et Caulis Opuntiae Dillenii
The addition of alcohol extract is 5.0ml/L-7.5ml/L.
The invention also discloses the preparation method of a kind of antistaling agent improving Carnation Flower Vase time, including following step
Rapid:
1) Radix et Caulis Opuntiae Dillenii water extract is prepared；
2) Radix et Caulis Opuntiae Dillenii alcohol extract is prepared；
3) weigh: according to mass percent weighing following components: two kinds of lixiviating solution 12%-18% of Radix et Caulis Opuntiae Dillenii, Pericarpium Zanthoxyli 1%-
5%, Radix Notoginseng 0.5%-0.8%, Fructus Capsici 2%-6%, Rhizoma Gastrodiae 7%-15%, chitin 3%-7%, Radix Stemonae 0.5%-1.5%, fourth
Fragrant 0.5%-1.5%, Rhizoma Alpiniae Officinarum 0.5%-1.5%, kieselguhr 2%-6%, surplus are water；
4) by step 3) in load weighted each component mixing, prepare antistaling agent.
Further, step 1) in prepare Radix et Caulis Opuntiae Dillenii water extract particularly as follows:
1.1) pretreatment of Radix et Caulis Opuntiae Dillenii: the pretreatment of Radix et Caulis Opuntiae Dillenii: dry at Yin Liang, is adjusted to 35-40 DEG C by drying baker, dries 8-
16 hours, with powder beater by material disintegrating, crossing 15-25 mesh sieve, pack respectively, 4 DEG C save backup；
1.2) take the pulverizing sample of raw Radix et Caulis Opuntiae Dillenii, add water, put into and boil 20-again after 8-16h is soaked in 45-55 DEG C of tepidarium
40min, filter and remove residue, 1000ml volumetric flask constant volume, obtain plant lixiviating solution stock solution, cool down 4 DEG C and save backup, concentration is
0.02g/l。
Further, step 2) in prepare Radix et Caulis Opuntiae Dillenii alcohol extract particularly as follows:
2.1) pretreatment of Radix et Caulis Opuntiae Dillenii: dry at Yin Liang, drying baker is adjusted to 35-40 DEG C, dry 8-16 hour, use powder beater
By material disintegrating, crossing 15-25 mesh sieve, pack respectively, 4 DEG C save backup；
2.2) taking the Radix et Caulis Opuntiae Dillenii plant sample of pulverizing, add the ethanol that quantitative volumn concentration is 95%, standard is
According to dipped more than the sample 4cm of sample volume, soak, put into concussion 45-51h in shaking table；Decompression sucking filtration, obtains filtrate and residue；
2.3) adding volumn concentration in the residue carried for the first time is 95% ethanol, just submergence sample 1cm, leaching
Bubble, puts into concussion 20-28h between shaking table；Decompression sucking filtration, obtains filtrate and residue；
2.4) in above-mentioned residue, add 95% ethanol, just submergence sample, soak, put into concussion 8-16h between shaking table；
Decompression sucking filtration, obtains filtrate, and residue discards；
2.5) concentrate: merging three filtrates, carry out concentrating under reduced pressure, bath temperature is 20-30 DEG C；
2.6) constant volume: with volumn concentration be 95% ethanol in concentrated solution constant volume to 20ml scale test tube, quality is dense
Degree, for containing the material of the dry plant sample of 1g in 1g/ml, i.e. 1ml extract, puts into 0～4 DEG C of preservation in refrigerator after sealing, standby
With.
Compared with prior art, the present invention can obtain and include techniques below effect:
1) two kinds of lixiviating solution of Radix et Caulis Opuntiae Dillenii can delay the aging of Carnation Flower, extends Vase time.Variable concentrations
Radix et Caulis Opuntiae Dillenii lixiviating solution different to the fresh-keeping effect of Carnation Flower, fresh-keeping effect that Radix et Caulis Opuntiae Dillenii 7.5ml/L alcohol extract processes
The most best.
2) in the physical signs measured, the physical signs such as pH value, electrical conductivity, TDS and salinity can not directly reflect health
Being fragrant fresh-keeping effect, the Dianthus carryophyllus petal POD activity of different disposal has close contacting, peroxide with the fresh-keeping of Dianthus carryophyllus
Enzyme is as the aging a kind of physical signs of plant tissue, and its major function is to remove free radical, antioxidation, slow down aging.This says
Bright for cutflower preservation, in its plant, POD activity is the strongest, is more conducive to extending cutting flower life, the most fresh-keeping.
3) comparison of POD activity value, in different disposal, Radix et Caulis Opuntiae Dillenii 7.5ml/L alcohol extract is corresponding with control treatment
POD activity is all in mild state, and the POD activity that other process has the trend of rising.Therefore deducing that, POD activity is basic
Keep balance, beneficially cutflower preservation.The process therefore deducing that Radix et Caulis Opuntiae Dillenii 7.5ml/L alcohol extract is most beneficial for health
It it is fragrant cutflower preservation.
Certainly, the arbitrary product implementing the present invention it is not absolutely required to reach all the above technique effect simultaneously.
Detailed description of the invention
Embodiments of the present invention are described in detail, thereby to the present invention how application technology hands below in conjunction with embodiment
Section solves technical problem and reaches the process that realizes of technology effect and can fully understand and implement according to this.
The present invention provides a kind of antistaling agent improving Carnation Flower Vase time, according to mass percent by following components
Constitute: two kinds of lixiviating solution 12%-18% of Radix et Caulis Opuntiae Dillenii, Pericarpium Zanthoxyli 1%-5%, Radix Notoginseng 0.5%-0.8%, Fructus Capsici 2%-6%, Rhizoma Gastrodiae
7%-15%, chitin 3%-7%, Radix Stemonae 0.5%-1.5%, Flos Caryophylli 0.5%-1.5%, Rhizoma Alpiniae Officinarum 0.5%-1.5%, diatom
Soil 2%-6%, surplus are water.
Described Radix et Caulis Opuntiae Dillenii lixiviating solution includes Radix et Caulis Opuntiae Dillenii water extract and Radix et Caulis Opuntiae Dillenii alcohol extract, wherein,
Radix et Caulis Opuntiae Dillenii water extract is prepared by the following method and obtains:
1) pretreatment of Radix et Caulis Opuntiae Dillenii: dry at Yin Liang, is adjusted to 35-40 DEG C by drying baker, dries 12 hours, with powder beater by material
Material is pulverized, and crosses 20 mesh sieves, packs respectively, and 4 DEG C save backup；
2) take the pulverizing sample 20g of raw Radix et Caulis Opuntiae Dillenii, add 1000ml water, put into and boil again after 12h is soaked in 50 DEG C of tepidariums
30min, filter and remove residue, 1000ml volumetric flask constant volume, obtain plant lixiviating solution stock solution, cool down 4 DEG C and save backup, concentration is
0.02g/l。
Radix et Caulis Opuntiae Dillenii alcohol extract prepares in the following manner:
1) pretreatment of Radix et Caulis Opuntiae Dillenii: dry at Yin Liang, is adjusted to 35-40 DEG C by drying baker, dries 12 hours, with powder beater by material
Material is pulverized, and crosses 20 mesh sieves, packs respectively, and 4 DEG C save backup；
2) weighing the Radix et Caulis Opuntiae Dillenii plant sample 50g of pulverizing, add 95% quantitative ethanol, standard is to soak according to sample volume
Cross more than sample 4cm, soak, put into concussion 48h in shaking table.Decompression sucking filtration, obtains filtrate and residue；
3) in the residue carried for the first time, add appropriate 95% ethanol, just submergence sample 1cm, soak, put into shaking table
Between shake 24h.Decompression sucking filtration, obtains filtrate and residue；
4) in above-mentioned residue, appropriate 95% ethanol is added, just submergence sample, soaks, puts into concussion 12h between shaking table.
Decompression sucking filtration, obtains filtrate, and residue discards
5) concentrate: merging three filtrates, carry out concentrating under reduced pressure, bath temperature is about 25 DEG C；
6) constant volume: with 95% ethanol in concentrated solution constant volume to 20ml scale test tube, mass concentration is that (i.e. 1ml carries 1g/ml
Take the material containing the dry plant sample of 1g in thing), put into 0～4 DEG C of preservation in refrigerator after sealing, standby.
Embodiment 1
The preparation method of a kind of antistaling agent improving Carnation Flower Vase time, comprises the following steps: Radix et Caulis Opuntiae Dillenii is cloudy
Liang Chu dries, and drying baker is adjusted to 35-40 DEG C, dries 12 hours, with powder beater by material disintegrating, crosses 20 mesh sieves, packs respectively, 4
DEG C save backup；Weighing the Radix et Caulis Opuntiae Dillenii plant sample 50g of pulverizing, add 95% quantitative ethanol, standard is according to sample volume
Dipped more than sample 4cm, soaks, and puts into concussion 48h in shaking table.Decompression sucking filtration, obtains filtrate and residue；Residual carried for the first time
Slag adds appropriate 95% ethanol, just submergence sample 1cm, soaks, put into concussion 24h between shaking table.Decompression sucking filtration, obtain filtrate and
Residue；In above-mentioned residue, add appropriate 95% ethanol, just submergence sample, soak, put into concussion 12h between shaking table.Decompression
Sucking filtration, obtains filtrate, and residue discards；Merging three filtrates, carry out concentrating under reduced pressure, bath temperature is about 25 DEG C；Use 95% ethanol
In concentrated solution constant volume to 20ml scale test tube, mass concentration is that 1g/ml is (i.e. containing the dry plant sample of 1g in 1ml extract
Material), put into 0～4 DEG C of preservation in refrigerator after sealing, standby.According to mass percent weighing following components: Radix et Caulis Opuntiae Dillenii lixiviating solution
15%, Pericarpium Zanthoxyli 3%, Radix Notoginseng 0.6%, Fructus Capsici 4%, Rhizoma Gastrodiae 11%, chitin 5%, the Radix Stemonae 1.0%, Flos Caryophylli 1.0%, Rhizoma Alpiniae Officinarum
1.0%, kieselguhr 4%, surplus are water；Load weighted each component is mixed, prepares antistaling agent.Radix et Caulis Opuntiae Dillenii alcohol extract
Addition be 7.5ml/L.
Embodiment 2
The preparation method of a kind of antistaling agent improving Carnation Flower Vase time, comprises the following steps: Radix et Caulis Opuntiae Dillenii is cloudy
Liang Chu dries, and drying baker is adjusted to 35 DEG C, dries 16 hours, with powder beater by material disintegrating, crosses 15 mesh sieves, packs respectively, 4 DEG C of guarantors
Deposit standby；Taking the Radix et Caulis Opuntiae Dillenii plant sample of pulverizing, add the ethanol that quantitative volumn concentration is 95%, standard is according to sample
Dipped more than the sample 4cm of product volume, soaks, and puts into concussion 51h in shaking table；Decompression sucking filtration, obtains filtrate and residue；Carry for the first time
Adding volumn concentration in the residue crossed is 95% ethanol, and just submergence sample 1cm soaks, and puts into concussion 20h between shaking table；
Decompression sucking filtration, obtains filtrate and residue；In above-mentioned residue, add 95% ethanol, just submergence sample, soak, put between shaking table
Concussion 16h；Decompression sucking filtration, obtains filtrate, and residue discards；Merging three filtrates, carry out concentrating under reduced pressure, bath temperature is 20 DEG C；With
Volumn concentration be 95% ethanol in concentrated solution constant volume to 20ml scale test tube, mass concentration is 1g/ml, i.e. 1ml extract
Thing contains the material of the dry plant sample of 1g, after sealing, puts into 0～4 DEG C of preservation in refrigerator, standby.Weigh according to mass percent
Following components: Radix et Caulis Opuntiae Dillenii lixiviating solution 12%, Pericarpium Zanthoxyli 5%, Radix Notoginseng 0.5%, Fructus Capsici 6%, Rhizoma Gastrodiae 7%, chitin 7%, the Radix Stemonae
0.5%, Flos Caryophylli 1.5%, Rhizoma Alpiniae Officinarum 0.5%, kieselguhr 6%, surplus are water；Load weighted each component is mixed, prepares
Antistaling agent.The addition of Radix et Caulis Opuntiae Dillenii alcohol extract is 5.0ml/L.
Embodiment 3
The preparation method of a kind of antistaling agent improving Carnation Flower Vase time, comprises the following steps: Radix et Caulis Opuntiae Dillenii is cloudy
Liang Chu dries, and drying baker is adjusted to 40 DEG C, dries 8 hours, with powder beater by material disintegrating, crosses 25 mesh sieves, packs respectively, 4 DEG C of guarantors
Deposit standby；Taking the Radix et Caulis Opuntiae Dillenii plant sample of pulverizing, add the ethanol that quantitative volumn concentration is 95%, standard is according to sample
Dipped more than the sample 4cm of product volume, soaks, and puts into concussion 45h in shaking table；Decompression sucking filtration, obtains filtrate and residue；Carry for the first time
Adding volumn concentration in the residue crossed is 95% ethanol, and just submergence sample 1cm soaks, and puts into concussion 28h between shaking table；
Decompression sucking filtration, obtains filtrate and residue；In above-mentioned residue, add 95% ethanol, just submergence sample, soak, put between shaking table
Concussion 8h；Decompression sucking filtration, obtains filtrate, and residue discards；Merging three filtrates, carry out concentrating under reduced pressure, bath temperature is 30 DEG C；Use body
Long-pending percentage composition be 95% ethanol in concentrated solution constant volume to 20ml scale test tube, mass concentration is 1g/ml, i.e. 1ml extract
In containing the material of the dry plant sample of 1g, put into 0～4 DEG C of preservation in refrigerator after sealing, standby.According to mass percent weigh with
Lower component: Radix et Caulis Opuntiae Dillenii lixiviating solution 18%, Pericarpium Zanthoxyli 1%, Radix Notoginseng 0.8%, Fructus Capsici 2%, Rhizoma Gastrodiae 15%, chitin 3%, the Radix Stemonae
1.5%, Flos Caryophylli 0.5%, Rhizoma Alpiniae Officinarum 1.5%, kieselguhr 2%, surplus are water；Load weighted each component is mixed, prepares
Antistaling agent.The addition of Radix et Caulis Opuntiae Dillenii alcohol extract is 6.0ml/L.
Embodiment 4
The preparation method of a kind of antistaling agent improving Carnation Flower Vase time, comprises the following steps: Radix et Caulis Opuntiae Dillenii is cloudy
Liang Chu dries, and drying baker is adjusted to 35 DEG C, dries 12 hours, with powder beater by material disintegrating, crosses 20 mesh sieves, packs respectively, 4 DEG C of guarantors
Deposit standby；Take the pulverizing sample 20g of raw Radix et Caulis Opuntiae Dillenii, add 1000ml water, put into and boil again after 12h is soaked in 50 DEG C of tepidariums
30min, filter and remove residue, 1000ml volumetric flask constant volume, obtain plant lixiviating solution stock solution, cool down 4 DEG C and save backup, concentration is
0.02g/l；According to mass percent weighing following components: Radix et Caulis Opuntiae Dillenii lixiviating solution 13%, Pericarpium Zanthoxyli 4%, Radix Notoginseng 0.6%, Fructus Capsici
5%, Rhizoma Gastrodiae 9%, chitin 6%, the Radix Stemonae 0.8%, Flos Caryophylli 1.2%, Rhizoma Alpiniae Officinarum 0.8%, kieselguhr 3%, surplus are water；To claim
Measured each component mixes, and prepares antistaling agent.The addition of described Radix et Caulis Opuntiae Dillenii water extract accounts for the volume of total liquid for immersing in bottle and contains
The 40% of amount.
Embodiment 5
The preparation method of a kind of antistaling agent improving Carnation Flower Vase time, comprises the following steps: Radix et Caulis Opuntiae Dillenii is cloudy
Liang Chu dries, and drying baker is adjusted to 40 DEG C, dries 8 hours, with powder beater by material disintegrating, crosses 25 mesh sieves, packs respectively, 4 DEG C of guarantors
Deposit standby；Take the pulverizing sample of raw Radix et Caulis Opuntiae Dillenii, add water, put into and after 12h is soaked in 45 DEG C of tepidariums, boil 30min again, cross and filter off
Slag, 1000ml volumetric flask constant volume, obtain plant lixiviating solution stock solution, cool down 4 DEG C and save backup, concentration is 0.02g/l；According to quality
Percentage ratio weighing following components: Radix et Caulis Opuntiae Dillenii lixiviating solution 17%, Pericarpium Zanthoxyli 2%, Radix Notoginseng 0.7%, Fructus Capsici 3%, Rhizoma Gastrodiae 12%, chitin
4%, the Radix Stemonae 1.2%, Flos Caryophylli 0.8%, Rhizoma Alpiniae Officinarum 1.2%, kieselguhr 5%, surplus are water；Load weighted each component is mixed,
Prepare antistaling agent.The addition of described Radix et Caulis Opuntiae Dillenii water extract accounts for the 20% of the volume content of total liquid for immersing in bottle.
The technique effect of the present invention is described below in conjunction with Radix et Caulis Opuntiae Dillenii lixiviating solution:
1, materials and methods
1.1 experiment flower materials
This experiment, selects the red Dianthus carryophyllus (Dianthus caryophyllus) on the occasion of bud stage to purchase as experiment flower material
From Dou Nan flowers market, blade is without the basic neat and consistent of pest and disease damage and character, totally 81 strain.
1.2 experiment Chinese crude drugs
This experiment, with Radix et Caulis Opuntiae Dillenii as material, picks up from park, Heilongtang.
2 experimental apparatus and reagent
2.1 experimental apparatus
The key instrument that this experiment is used: plug bottle, shaking table, triangular flask, water bath, refrigerator, electronic balance, filter paper, amount
Cup, beaker, Glass rod, funnel, volumetric flask, microscope slide, blood counting chamber, optical microscope, centrifuge, conductivity meter, liquid-transfering gun,
Pulverizer, drying baker, 0.05mol/L PH7.8 phosphate buffer etc..
2.2 experiment reagent
0.1MpH7.8 sodium phosphate (NaH2PO4-NaH2PO4) buffer
A(0.1M Na2HPO4Solution): Na2HPO·412H2O (MW=358.14) 17.9g is in 500mldH2O (distilled water)
Fully mixing.
B(0.1M NaH2PO4Solution): NaH2PO4·2H2O (MW=156.01) 7.8g is in 500mldH2O (distilled water) fills
Divide mixing.
A liquid and B liquid 91.5:8.5 are the PBS of 0.1MpH7.8 after fully mixing.Put in 4 DEG C of refrigerators and preserve,
Standby.
A liquid and B liquid are the mother solution of differently configured pH value buffer.
POD reaction mixture (adds the heating of 112ul guaiacol, cooling in the phosphate buffer of 250ml0.1MpH6.0
Rear addition 76ul30%H2O2)
3 experimental designs
3.1 water extract designs
Total liquid for immersing in bottle is 300ml, takes former extracting solution respectively and mixes with water and be made into 20% and (extract stock solution and account for total liquid for immersing in bottle and be
20%, lower with), 40%, 60% and 80%4 gradients, each gradient sets 3 repetitions, each repeat to process 3 strain Dianthus carryophyllus and cuts
Flower.
3.2 organic solvent lixiviating solution
Total liquid for immersing in bottle is 300ml, and (1L liquid for immersing in bottle kind is organic containing 2.5ml with ethanol configuration 2.5ml/L to take former extracting solution respectively
Extraction stock solution, lower with), 3 gradients of 5.0ml/L and 7.5ml/L, each gradient sets 3 repetitions, and each repetition processes 3 strains
Carnation Flower.
4 experimental procedures
4.1 preparations improve the antistaling agent of Carnation Flower Vase time
4.2 Dianthus carryophyllus pretreatment
Choosing the Dianthus carryophyllus bud stage cut-flower just opened, its degree of opening is basically identical, and long about the 30cm of spray, scape is sturdy
Straight and upright cut-flower requires that flower pattern is neat, Hua Jing 7-12cm, and pattern is bright-coloured, causes harm without disease pest, and leaf is dark green, and stem is logical straight, high more than 80cm,
When cutting spray with sharp cutter, otch is preferably whittled into the inclined-plane of 45 degree, so can expand water suction area, weigh every flower
Initial weight.
4.3 Carnation Flowers enter bottle
Inserting 3 processed cut-flowers in each plug bottle, under noting during inserting cut-flower, clip does not have bubble to go out
Existing, keep the distance between each vase, it is impossible to allow it mutually extrude, bottleneck preservative film seals.Become 120 ° between each ZHUHUA branch, put
Under room temperature environment, ventilate without direct sunlight, periodically adjust the position of vase.
4.4 bottles of slotting Dianthus carryophyllus mode of appearance are observed
Observed once every 2 days, main detection blade and the growth of flower, developmental state with, according to all previous observed knot
Fruit carries out corresponding record.Bottled sustaining time is wilted with petal internal layer, curling, and outer layer is shrivelled or rolls over stem, and curved stem angle is more than 90
Degree, losing ornamental value is standard.Bottled sustaining time all drops with petal, blade all turns yellow withered till.
The mensuration of 4.5 Dianthus carryophyllus liquid for immersing in bottle pH value
Measure every three days once, dip a little liquid for immersing in bottle with Glass rod and drip on PH reagent paper, compared with colorimetric card relatively after
Carry out reading.Glass rod will be cleaned with distilled water and dry with clean paper, to ensure data dipping reagent before every time
Accuracy.Compare with colorimetric card after stable and carry out reading.Glass rod will be cleaned with distilled water before dipping liquid for immersing in bottle every time,
Dry, it is ensured that the accuracy of data.
4.6 Dianthus carryophyllus liquid for immersing in bottle electrical conductivity, salinity and the mensuration of TDS
TDS is the aggregative indicator of a test agent cleanliness, and the size of TDS numerical value illustrates the size of solution concentration, and it can
It is considered to combine a total value of electrical conductivity, micro organism quantity and solution impurity with approximate.This experiment is to Radix et Caulis Opuntiae Dillenii water logging
The liquid for immersing in bottle of extract alcohol extract variable concentrations is TDS and measures.
Concrete measuring process is as follows: is first connected by instrument electricity, then plugs in, probe pen is inserted tested Radix et Caulis Opuntiae Dillenii water logging
In the liquid for immersing in bottle of extract and alcohol extract variable concentrations, then open instrument switch, in case probe pen energising oxidation by air, press
Mode button switches and is successively read three desired values record.It should be noted that probe pen in test, do not contact bottle inwall and
Go to the bottom, test keeps fixing.Often survey one, probe pen has been put in distilled water and cleans up, then after paper using is dried,
Can measure next time.
The mensuration of 4.7 bottles of slotting Dianthus carryophyllus petal POD enzymatic activitys
Surveyed once every 2 days, win each concentration difference and repeat bottle and insert 1～2 relatively good petal on cut-flower and put into
In corresponding sack, put in cold closet standby.After treating that conventional index measures, it is drawn off, weighs 0.5 blade in grinding
In alms bowl, add liquid nitrogen, grind rapidly, load centrifugal after adding the PBS extracting solution mix homogeneously of 5mlpH=7.8 after grinding completely
Guan Zhong, takes out after centrifugal 15 minutes in being placed on centrifuge, centrifugal clear liquid loads cold preservation in corresponding centrifuge tube,
Standby.
The mensuration of petal POD activity: first extract POD reaction mixture 3.9ml with liquid-transfering gun and inject in cuvette, then will
Centrifugal liquid after cold preservation is taken out, and takes its supernatant about 0.1ml with liquid-transfering gun and injects in same cuvette, cover after it melts
Lid puts into the mensuration carrying out enzyme reaction speed in spectrophotometer after being shaken up, each concentration is each to be repeated three times,
Its meansigma methods is sought after record.
POD enzymatic activity result calculates: peroxidase activity=(△ A470*VT)*(WF*VS*t)-1
In above formula: △ A470Represent the change of light absorption value in the response time；
WFRepresent petal weight (g)；
T represents the response time (min)；
VTRepresent and extract enzyme liquid cumulative volume (ml)；
VSEnzyme liquid is taken long-pending (ml) when representing mensuration.
5 results and analysis
5.1 different disposal Dianthus carryophyllus mode of appearance are analyzed
The change of table 1 Dianthus carryophyllus liquid for immersing in bottle mode of appearance is compared
Every 2 days record upright degree of spica with degree of opening tabulation as above, as can be seen from Table 1, at Radix et Caulis Opuntiae Dillenii cut-flower
Bottle is inserted period, has 5 effects processed to be better than comparison, it was demonstrated that two kinds of lixiviating solution are all sent out Carnation Flower is fresh-keeping in 7 process
The effect of having given birth to.The Dianthus carryophyllus that the various variable concentrations of Radix et Caulis Opuntiae Dillenii water extract and alcohol extract process May 25 when
Here cut-flower major part withers the most, and Radix et Caulis Opuntiae Dillenii 7.5ml/L has here 35% the most do not wither, Radix et Caulis Opuntiae Dillenii 2.5ml/L May 22 time
Time is withered the most serious with spray bending.By the open hour of relatively flower, spray degree of crook situation, petal withers here journey
Spending, the last death time of flower can be seen that two kinds of different Radix et Caulis Opuntiae Dillenii lixiviating solution total fresh-keeping effect alcohol extracts are better than water logging
Extract.The fresh-keeping effect analyzing further two kinds of lixiviating solution variable concentrations finds, the fresh-keeping effect of alcohol extract 7.5ml/L is
Good, alcohol extract 2.5ml/L and 60% water extract fresh-keeping effect are worst.
5.2 Radix et Caulis Opuntiae Dillenii water extracts and alcohol extract variable concentrations pH value compare
PH value reflection is solution acid alkalinity, and peracidity and high alkalinity solution are all unfavorable for the water suction of cut-flower, and pH value becomes
Changing and do not have certain rule, have falls after rising, and rises after the first fall having, but change is the most inconspicuous.Radix et Caulis Opuntiae Dillenii 7.5ml/L bottle is inserted
Changing between the pH value 6 to 7 of liquid, change minimum among several process, fresh-keeping effect is best；Radix et Caulis Opuntiae Dillenii 80% water extract its
PH value prolongation over time, amplitude of variation ranges up to more greatly 8.0 minimum about 5.6, and fresh-keeping effect is worst.
5.3 liquid for immersing in bottle conductivity variations
The size of electrical conductivity reflects the size of effects of ion concentration, and the size of ion concentration indirectly illustrates cell
The destructiveness of film.The ion concentration contained in view of the fresh-keeping liquid of different disposal itself is different, thus we compare conductance
The dynamic change of rate.Prolongation over time, conductivity variations, show that prolongation cell membrane disruption degree over time is gradually
Change.The computer rate of various process, with the presence of chemical ion in Radix et Caulis Opuntiae Dillenii lixiviating solution, thus compares merely electrical conductivity and does not has reality
Meaning.Therefore we analyze the size that electrical conductivity rises, the measurement film destructiveness approximated.By relatively several different places
Reason we have found that the change curve ratio of Radix et Caulis Opuntiae Dillenii 7.5ml/L fresh-keeping liquid is shallower, illustrates that this process can reduce cell membrane
Impaired, the Vase time of cut-flower can be increased.2.5ml/L alcohol extract and 80% water extract amplitude of variation are unfavorable for more greatly
Carnation Flower is fresh-keeping.
5.4 liquid for immersing in bottle TDS changes
TDS is the aggregative indicator of a test cleanliness, and TDS is the biggest, illustrates that solution concentration is the biggest, and what it can approximate thinks
It it is the total value combining electrical conductivity, micro organism quantity and solution impurity.The TDS of the various process of Radix et Caulis Opuntiae Dillenii has becoming of rising
Gesture, illustrates the prolongation along with fresh keeping time, and TDS is increasing.The TDS value of various process changes in addition to Radix et Caulis Opuntiae Dillenii (7.5ml/L)
The most obvious, various concentration process because its variation tendency of difference of solution concentration is the most different, and ascendant trend can be seen that
Minimum (the 80mg/L 85mg/L 90mg/L 100mg/L) fresh-keeping effect of Radix et Caulis Opuntiae Dillenii 7.5ml/L ascendant trend is best.Radix et Caulis Opuntiae Dillenii
Water extract 20% (60mg/L 85mg/L 100mg/L 120mg/L) and Radix et Caulis Opuntiae Dillenii 2.5ml/L (50mg/L 60mg/
L 90mg/L 120mg/L) variation tendency of alcohol extract is maximum, and fresh-keeping effect is worst.
5.5 liquid for immersing in bottle salinity altercation
A lot of inorganic salt such as inorganic salt such as potassium salt, calcium salt, aluminium salt, mantoquita, nickel salt and zinc salt etc. can increase the infiltration of solution
Gesture and the turgescence of petal cell, the activity of microorganism, the beneficially holding of spray water balance in suppression aqueous solution, extend bottle and insert
Life-span.Salinity is inversely proportional to fresh-keeping effect, i.e. the biggest fresh-keeping effect of salinity is the poorest, and concrete reason need to grind further
Study carefully.
5.6 peroxidase (POD) expression activitiy
Peroxidase is as the aging a kind of physical signs of plant tissue, and its major function is to remove free radical, antioxygen
Change, slow down aging.This explanation is for cutflower preservation, and in its plant, POD activity is the strongest, is more conducive to extending the cut-flower longevity
Life, the most fresh-keeping.
In first three sky of preservation process, each POD activity processed is the most in rising trend, and enzymatic activity is in the ripple of big rise and big fall
Dynamic state is obviously unfavorable for fresh-keeping, and compares the POD activity corresponding with Radix et Caulis Opuntiae Dillenii 7.5ml/L in addition to first three sky is changed significantly
(5.2-5.5-6.0), later variation tendency, generally in plateau (5.8-5.9-6.1), illustrates that POD activity is basic and keeps
Balance, beneficially cutflower preservation.The rising that later variation tendency has, some decline is started as from the 6th day.
6 conclusions and discussion
Cut-flower is the live body that a class cuts off parent, still provides for the metabolic activities such as transpiration, breathing after adopting.Cut-flower departs from parent
After, its nutrient source is cut off, and adds envirment factor and the harmful effect of microorganism and the internal a series of Physiology and biochemistries occurred thereof
Change, ultimately results in and cuts flower withering and wither.The Vase time of cut-flower and bottle are inserted into point in close relations, and suitable liquid for immersing in bottle can change
The character such as kind cut-flower fresh weight, pattern, bennet flexibility, can delay the aging of cut-flower, keep viewing and admiring of long period, improve cut-flower
Ornamental quality and economic worth.The fewest to the research report of cutflower preservation with extract of Chinese traditional medicine, and the most do not act on machine
Reason is analyzed.The present invention has inquired into the Radix et Caulis Opuntiae Dillenii difference water extract in antistaling agent and the alcohol extract shadow to Carnation Flower
Ring, and the relevant physical signs that analyzes and cut flower withering.From the point of view of result of the test, Radix et Caulis Opuntiae Dillenii water extract and alcohol extract
Variable concentrations process and all delayed the aging of Carnation Flower, extend Vase time.Find the guarantor of alcohol extract simultaneously
Fresh effect is better than water extract.The most best with ethanol 7.5ml/L effect.But find in process of the test, have concentration higher
Suspicion, other concentration of water extract and alcohol extract processes the difficulty that may absorb water because of excessive concentration, should concentration be dropped
Low.In order to analyze the reason producing these results, do again POD value, pH value, salinity and electrical conductivity analysis, find Radix et Caulis Opuntiae Dillenii
Two kinds of lixiviating solution can suppress the growth of microorganism, reduce film damage, increase salinity.This is that two kinds of lixiviating solution of Radix et Caulis Opuntiae Dillenii are sent out
The mechanism of raw effect.
Described above illustrate and describes some preferred embodiments of invention, but as previously mentioned, it should be understood that invention is not
It is confined to form disclosed herein, is not to be taken as the eliminating to other embodiments, and can be used for other combinations various, amendment
And environment, and can be carried out by above-mentioned teaching or the technology of association area or knowledge in invention contemplated scope described herein
Change.And the change that those skilled in the art are carried out and change are without departing from the spirit and scope of invention, the most all should weigh appended by invention
In the protection domain that profit requires.
Claims (8)
1. the antistaling agent improving Carnation Flower Vase time, it is characterised in that according to mass percent by following components
Constitute: Radix et Caulis Opuntiae Dillenii lixiviating solution 12%-18%, Pericarpium Zanthoxyli 1%-5%, Radix Notoginseng 0.5%-0.8%, Fructus Capsici 2%-6%, Rhizoma Gastrodiae 7%-
15%, chitin 3%-7%, Radix Stemonae 0.5%-1.5%, Flos Caryophylli 0.5%-1.5%, Rhizoma Alpiniae Officinarum 0.5%-1.5%, kieselguhr
2%-6%, surplus are water.
The antistaling agent of raising Carnation Flower Vase time the most according to claim 1, it is characterised in that described Radix et Caulis Opuntiae Dillenii
Lixiviating solution includes Radix et Caulis Opuntiae Dillenii water extract and Radix et Caulis Opuntiae Dillenii alcohol extract.
The antistaling agent of raising Carnation Flower Vase time the most according to claim 2, it is characterised in that described Radix et Caulis Opuntiae Dillenii
Water extract is prepared by the following method and obtains:
1) pretreatment of Radix et Caulis Opuntiae Dillenii: dry at Yin Liang, is adjusted to 35-40 DEG C by drying baker, dries 8-16 hour, with powder beater by material
Pulverizing, cross 15-25 mesh sieve, pack respectively, 4 DEG C save backup；
2) take the pulverizing sample of raw Radix et Caulis Opuntiae Dillenii, add water, put into and boil 20-40min again after 8-16h is soaked in 45-55 DEG C of tepidarium,
Filter and remove residue, 1000ml volumetric flask constant volume, obtain plant lixiviating solution stock solution, cool down 4 DEG C and save backup, concentration is 0.02g/l.
The antistaling agent of raising Carnation Flower Vase time the most according to claim 2, it is characterised in that described Radix et Caulis Opuntiae Dillenii
Alcohol extract prepares in the following manner:
1) pretreatment of Radix et Caulis Opuntiae Dillenii: dry at Yin Liang, is adjusted to 35-40 DEG C by drying baker, dries 8-16 hour, with powder beater by material
Pulverizing, cross 15-25 mesh sieve, pack respectively, 4 DEG C save backup；
2) taking the Radix et Caulis Opuntiae Dillenii plant sample of pulverizing, add the ethanol that quantitative volumn concentration is 95%, standard is according to sample
Dipped more than the sample 4cm of product volume, soaks, and puts into concussion 45-51h in shaking table；Decompression sucking filtration, obtains filtrate and residue；
3) adding volumn concentration in the residue carried for the first time is 95% ethanol, and just submergence sample 1cm soaks, puts
Enter concussion 20-28h between shaking table；Decompression sucking filtration, obtains filtrate and residue；
4) in above-mentioned residue, add 95% ethanol, just submergence sample, soak, put into concussion 8-16h between shaking table；Decompression is taken out
Filter, obtains filtrate, and residue discards；
5) concentrate: merging three filtrates, carry out concentrating under reduced pressure, bath temperature is 20-30 DEG C；
6) constant volume: with volumn concentration be 95% ethanol in concentrated solution constant volume to 20ml scale test tube, mass concentration is 1g/
Ml, i.e. 1ml extract contains the material of the dry plant sample of 1g, after sealing, puts into 0～4 DEG C of preservation in refrigerator, standby.
The antistaling agent of raising Carnation Flower Vase time the most according to claim 2, it is characterised in that described Radix et Caulis Opuntiae Dillenii
The addition of water extract accounts for the 20%-40% of the volume content of total liquid for immersing in bottle, and the addition of Radix et Caulis Opuntiae Dillenii alcohol extract accounts for always
The 5.0ml/L-7.5ml/L of liquid for immersing in bottle.
6. the preparation method of the antistaling agent improving Carnation Flower Vase time, it is characterised in that comprise the following steps:
1) Radix et Caulis Opuntiae Dillenii water extract is prepared；
2) Radix et Caulis Opuntiae Dillenii alcohol extract is prepared；
3) weigh: according to mass percent weigh following components: two kinds of lixiviating solution 12%-18% of Radix et Caulis Opuntiae Dillenii, Pericarpium Zanthoxyli 1%-5%,
Radix Notoginseng 0.5%-0.8%, Fructus Capsici 2%-6%, Rhizoma Gastrodiae 7%-15%, chitin 3%-7%, Radix Stemonae 0.5%-1.5%, Flos Caryophylli
0.5%-1.5%, Rhizoma Alpiniae Officinarum 0.5%-1.5%, kieselguhr 2%-6%, surplus are water；
4) by step 3) in load weighted each component mixing, prepare antistaling agent.
The preparation method of the antistaling agent of raising Carnation Flower Vase time the most according to claim 6, it is characterised in that
Described step 1) in prepare Radix et Caulis Opuntiae Dillenii water extract particularly as follows:
1.1) pretreatment of Radix et Caulis Opuntiae Dillenii: the pretreatment of Radix et Caulis Opuntiae Dillenii: dry at Yin Liang, is adjusted to 35-40 DEG C by drying baker, dries 8-16 little
Time, with powder beater by material disintegrating, cross 15-25 mesh sieve, pack respectively, 4 DEG C save backup；
1.2) take the pulverizing sample of raw Radix et Caulis Opuntiae Dillenii, add water, put into and boil 20-again after 8-16h is soaked in 45-55 DEG C of tepidarium
40min, filter and remove residue, 1000ml volumetric flask constant volume, obtain plant lixiviating solution stock solution, cool down 4 DEG C and save backup, concentration is
0.02g/l。
The preparation method of the antistaling agent of raising Carnation Flower Vase time the most according to claim 6, it is characterised in that
Described step 2) in prepare Radix et Caulis Opuntiae Dillenii alcohol extract particularly as follows:
2.1) pretreatment of Radix et Caulis Opuntiae Dillenii: dry at Yin Liang, is adjusted to 35-40 DEG C by drying baker, dries 8-16 hour, with powder beater by material
Material is pulverized, and crosses 15-25 mesh sieve, packs respectively, and 4 DEG C save backup；
2.2) take the Radix et Caulis Opuntiae Dillenii plant sample of pulverizing, add the ethanol that quantitative volumn concentration is 95%, standard is basis
Dipped more than the sample 4cm of sample volume, soaks, and puts into concussion 45-51h in shaking table；Decompression sucking filtration, obtains filtrate and residue；
2.3) adding volumn concentration in the residue carried for the first time is 95% ethanol, and just submergence sample 1cm soaks,
Put into concussion 20-28h between shaking table；Decompression sucking filtration, obtains filtrate and residue；
2.4) in above-mentioned residue, add 95% ethanol, just submergence sample, soak, put into concussion 8-16h between shaking table；Decompression
Sucking filtration, obtains filtrate, and residue discards；
2.5) concentrate: merging three filtrates, carry out concentrating under reduced pressure, bath temperature is 20-30 DEG C；
2.6) constant volume: with volumn concentration be 95% ethanol in concentrated solution constant volume to 20ml scale test tube, mass concentration is
1g/ml, i.e. 1ml extract contains the material of the dry plant sample of 1g, after sealing, puts into 0～4 DEG C of preservation in refrigerator, standby.
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Cited By (6)
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| CN107711826A (en) * | 2017-12-01 | 2018-02-23 | 西南林业大学 | A kind of manufacture craft of the fresh-keeping reagent of the Carnation Flower of ginkgo extract |
| CN107743958A (en) * | 2017-12-01 | 2018-03-02 | 西南林业大学 | A kind of manufacture craft of the fresh-keeping reagent of the Cut Carnation Flower of garlic extract |
| CN107751191A (en) * | 2017-12-01 | 2018-03-06 | 西南林业大学 | A kind of manufacture craft of the fresh-keeping reagent of the Cut Carnation Flower of onions extracting liquid |
| CN107787962A (en) * | 2017-12-01 | 2018-03-13 | 西南林业大学 | A kind of manufacture craft of the fresh-keeping reagent of the Cut Carnation Flower of ginger extract |
| CN107926939A (en) * | 2017-12-01 | 2018-04-20 | 西南林业大学 | A kind of manufacture craft of the fresh-keeping reagent of Cut Carnation Flower of Chinese ilex extracting solution |
| CN111713489A (en) * | 2020-07-08 | 2020-09-29 | 郑州师范学院 | Cut flower preservative and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107711826A (en) * | 2017-12-01 | 2018-02-23 | 西南林业大学 | A kind of manufacture craft of the fresh-keeping reagent of the Carnation Flower of ginkgo extract |
| CN107743958A (en) * | 2017-12-01 | 2018-03-02 | 西南林业大学 | A kind of manufacture craft of the fresh-keeping reagent of the Cut Carnation Flower of garlic extract |
| CN107751191A (en) * | 2017-12-01 | 2018-03-06 | 西南林业大学 | A kind of manufacture craft of the fresh-keeping reagent of the Cut Carnation Flower of onions extracting liquid |
| CN107787962A (en) * | 2017-12-01 | 2018-03-13 | 西南林业大学 | A kind of manufacture craft of the fresh-keeping reagent of the Cut Carnation Flower of ginger extract |
| CN107926939A (en) * | 2017-12-01 | 2018-04-20 | 西南林业大学 | A kind of manufacture craft of the fresh-keeping reagent of Cut Carnation Flower of Chinese ilex extracting solution |
| CN111713489A (en) * | 2020-07-08 | 2020-09-29 | 郑州师范学院 | Cut flower preservative and application thereof |
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Application publication date: 20161123 |