CN107723265A - A kind of Alteromonad and the biological flocculant prepared using the bacterium and preparation method - Google Patents
A kind of Alteromonad and the biological flocculant prepared using the bacterium and preparation method Download PDFInfo
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- CN107723265A CN107723265A CN201711135488.9A CN201711135488A CN107723265A CN 107723265 A CN107723265 A CN 107723265A CN 201711135488 A CN201711135488 A CN 201711135488A CN 107723265 A CN107723265 A CN 107723265A
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- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/5236—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using inorganic agents
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Abstract
The biological flocculant and preparation method prepared the present invention relates to a kind of Alteromonad and using the bacterium, the preservation date of Alteromonad is on July 25th, 2017, deposit number is CGMCC No.14457, the flocculence extracellular products obtained after the directly centrifugation of the tunning of the Alteromonad, as biological flocculant, the biological flocculant is mixed with the solution of copper ions, produces complex biological flocculant.The Alteromonad that the present invention filters out produces a kind of microbial flocculant by fermenting, and the microbial flocculant is easily isolated extraction, preparation method is simple, can effectively mitigate the workload of biological downstream engineering, reduce its cost, greatly improve the production efficiency of microbial flocculant.
Description
Technical field
The invention belongs to technical field of sewage, is related to a kind of Alteromonad and the bioflocculation prepared using the bacterium
Agent and preparation method.
Background technology
Current social, densely-populated, China's water resource is more and more in short supply, and with industrial technology high speed development,
Water quality also drastically reduces therewith, and water pollution is serious, and the processing for waste water is always popular research direction.Including biochemistry
The methods of method, absorption, ion exchange, chemical oxidation.Flocculation technique is wherein more universal, and cost is relatively low, and treatment effeciency compared with
One of high method.Its main function is that flocculant can pollute with large particulate matter in water, and pigment, heavy metal ion etc.
Thing combines, and then forms larger floccule body, is settled down to the bottom, so as to purifying water body.
Instantly, the conventional flocculant of water process mainly includes inorganic flocculating agent（Such as aluminium salt, molysite and its polymer）；Have
Machine high polymer coagulant（Such as polyacrylamide and its derivative）；Biological flocculant（Such as microbial flocculant）.Traditional flocculation
Although agent has preferable flocculation activity, but it is in use, can produce more noxious products, easily cause the secondary of environment
Pollution, and it is highly detrimental to the health of human body.On the other hand, the external application to part organic polymer coargulator has strict limit
System, this also cause microbial flocculant by its it is safe, environmentally friendly and degradable the characteristics of turn into developmental research instantly
Emphasis.
Microbial flocculant can be divided into three classes according to source, can be directly thin by the use of microorganism as flocculant, including part
Bacterium, actinomyces, saccharomycete etc.；It is glucan such as filamentous fungal cell wall, sweet using microorganism wall extract as flocculant
Reveal glycan, chitin etc.；Using microbial cell metabolite as flocculant, main component is polysaccharide, protein and its compound
Thing.
Microbial flocculant can gather the particulate matters such as solid suspended particle, colloidal particle and the somatic cells in system
Collection, sedimentation.But at present, the flocculation mechanism of microbial flocculant not yet understands, according to hypothesis, can be roughly divided into charge neutrality work
With the volume effect of sweeping, viral hypothesis and cement hypothesis etc..
Microbial flocculant has the advantages of other flocculants can not compare for water process, but scarce there is also part
Point.Currently, the domestic research level for microbial flocculant is relatively low, and its cost of manufacture is high, and assay method is single and micro- life
The specific aim of thing flocculant is not strong etc., and these shortcomings all affect microbial flocculant development.
The content of the invention
The technical problems to be solved by the invention are in view of the shortcomings of the prior art, there is provided a kind of Alteromonad and utilization
Biological flocculant and preparation method prepared by the bacterium.Alteromonad (Alteromonadales provided by the invention
bacterium）DN3-1, the Alteromonad produces a kind of microbial flocculant by fermenting, and the microbial flocculant is easy to
Separation and Extraction, preparation method is simple, can effectively mitigate the workload of biological downstream engineering, reduce its cost, greatly improve micro- life
The production efficiency of thing flocculant.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of Alteromonad, Alteromonad (Alteromonas sp.) G1-3, is deposited in China General Microbiological bacterium
Kind preservation administrative center, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and preservation date is July 25 in 2017
Day, deposit number is CGMCC No.14457.
Present invention also offers biological flocculant prepared by Alteromonad, the biological flocculant is by the Alteromonas
The flocculence extracellular products obtained after the directly centrifugation of the tunning of bacterium.
Present invention also offers complex biological flocculant prepared by Alteromonad, the complex biological flocculant includes life
The solution of thing flocculant and copper ions；The biological flocculant obtains after directly being centrifuged by the tunning of the Alteromonad
The flocculence extracellular products arrived；The copper ion solution that mass concentration before the solution compounding of the copper ions is 1%.
Present invention also offers the preparation method of biological flocculant, comprise the following steps：The Alteromonad is inoculated with
Into fermentation medium, shaking table culture, shaking speed 220rpm/min, cultivation temperature is 37 DEG C, after culture 16 to 20h, will be sent out
After zymotic fluid directly centrifuges, flocculence extracellular products is obtained, produces biological flocculant.Micro- life caused by the Alteromonad
Thing flocculant, the compound extracellular products of polysaccharide and albumen for the bacterium, its available direct visual perception, white cotton-shaped, sedimentation
To liquid bottom, it can directly centrifuge and obtain described microbial flocculant.
Preferably, the composition of the fermentation medium is：5-10g/L of peptone, 3-5g/L of beef extract, sodium chloride 2-
4g/L, surplus are water, and pH is 7.0-8.0.
Present invention also offers the preparation method of described complex biological flocculant, comprise the following steps：
（1）The Alteromonad is seeded in fermentation medium, shaking table culture, shaking speed 220rpm/min, culture temperature
Spend for 37 DEG C, after culture 16 to 20h, after zymotic fluid is directly centrifuged, obtain flocculence extracellular products, produce bioflocculation
Agent；
（2）The preparation of complex biological flocculant：Flocculence extracellular products samples 0.5-1mL, and the copper ion that mass concentration is 1% is molten
Liquid dosage is 1-3mL, the processing for 1L water samples.
Preferably, the composition of the fermentation medium is：5-10g/L of peptone, 3-5g/L of beef extract, sodium chloride 2-
4g/L, surplus are water, and pH is 7.0-8.0.
Beneficial effect
Compared with prior art, its microbial flocculant incubation time is short, and dosage is few by the present invention, and because its naked eyes is visible, precipitation
To bottom, extraction process is extremely simple quick, can greatly weaken production cost.
The microbial flocculant of the present invention is the extracellular products of Alteromonad, and main constituents are polysaccharide and albumen
Matter, its is easily decomposes, to environment non-secondary pollution.Copper category ion can strengthen the bridge linking effect of biological flocculant and neutralize to make
With, it is significant to the flocculation activity of biological flocculant, floccule body, and rapid subsidence can be quickly generated, flocculating effect is preferable.
Brief description of the drawings
Fig. 1 is the Alteromonad of the present invention（Alteromonadales bacterium DN3-1）The born of the same parents that G1-3 is produced
Outer product；
Fig. 2 is the morphological feature of the Alteromonad of the present invention.
Fig. 3 is the flocculating effect of the microbial flocculant of the invention of the present invention（A left side is control group, and the right side is experimental group）.
Fig. 4 is the flocculating rate contrast knot of the single microorganism flocculate of the present invention, composite flocculation agent and single copper ion
Fruit.
Fig. 5 is that the microbial flocculant of the present invention and high-efficiency sewage handle microbial inoculum and be jointly processed by design sketch of the sewage after 3 days
（A left side is control group, and the right side is experimental group）.
Embodiment
The invention will be further elucidated with reference to specific embodiments.It should be understood that these embodiments are merely to illustrate this hair
Bright rather than limitation the scope of the present invention.In addition, it is to be understood that after the content of the invention lectured has been read, art technology
Personnel can make various changes or modifications to the present invention, and these equivalent form of values equally fall within the application appended claims and limited
Fixed scope.
Embodiment 1
1st, the separation and culture of bacterial strain
Sampling：From sewage plant, 500mL activated sludge is sampled, after logical oxygen is tamed 7 days, static 30min, takes its supernatant.
0.1mL water samples are drawn from supernatant water sample with Sterile pipette, are accessed on meat soup solid medium, flat board coating,
With 37 DEG C in incubator, cultivate 2 days.
The uniform flat board of strains'distribution, the different bacterium colony of observation single bacterium colony form are chosen, then picking single bacterium colony turns respectively
To meat soup slant medium, purifying culture, incubation time 18h are carried out in 37 DEG C in incubator.
By separating obtained inoculation into fermentation medium, in 37 DEG C, 220rpm/min cultivates 18h, fermentation medium
Composition be：Peptone 6g/L, beef extract 4g/L, sodium chloride 3g/L, surplus are water, pH 7.5；Taken after nutrient solution fermented and cultured
Go out, stand, observation whether there is flocculent deposit generation, takes supernatant and flocculent deposit 1mL with dropper respectively, it is 4g/L to be added to concentration
50mL aqueous suspension ofkaolins in, using the solution of 1% calcium chloride as flocculation aid, add 2mL, stir 3min after, static 30min,
Observe flocculating effect.Its absorbance is determined under the 550nm wavelength of spectrophotometer；Added simultaneously with being not added with fermented liquid supernatant liquid
Concentration is 4g/L aqueous suspension ofkaolin as comparison liquid.
Flocculating rate calculation formula is
Flocculating rate=（A-B）/A*100%
Wherein, A is absorbance of the control group at 550nm, and B is absorbance of the experimental group at 550nm.
Wherein flocculating rate highest bacterial strain is selected, and the flocculating rate for contrasting its fermented liquid supernatant liquid and the extracellular products that flocculates is high
Low, the flocculence extracellular products that the Alteromonad is produced is specifically as shown in Figure 1；The morphological feature of the Alteromonad such as Fig. 2
It is shown, shown in the bacterial strain electron-microscope scanning figure, long 10 to 15 μm, wide 5 to 7.5 μm, colony shape is circle, and surface is smooth, flat
Plano-convex rises, neat in edge, surface wettability, white, opaque.For Gram-negative bacteria.It is 7.5 to 8.5 to grow pH, and temperature 30 arrives
37 DEG C of method for preserving：The 4 DEG C of preservations of meat soup solid slope culture medium.
By experiment, the flocculence extracellular products that the Alteromonad that the present invention chooses is produced has higher flocculation
Rate, flocculating rate can reach more than 96%, and specific experimental result is as shown in Figure 3（The left side is the control of 4g/L aqueous suspension ofkaolin
Liquid；Flocculating effect when the right uses for addition microbial flocculant）.
Single microorganism flocculate, composite flocculation agent and single copper caused by the Alteromonad that the present invention is chosen
The flocculating rate comparing result table of ion is as shown in Figure 4.
2nd, the method that microbial flocculant is prepared by Alteromonad：The Alteromonad is accessed in fermentation medium,
Taken out after 37 DEG C, 220rpm/min culture 18h nutrient solution fermented and cultureds, stand, centrifugation, take its cotton-shaped extracellular products, be made
Microbial flocculant.
The 3rd, obtained microbial flocculant is pressed to 2% access amount, access to institute of Tianjin Peiyang Biotrans Biotech Co., Ltd
In the high-efficiency sewage processing microbial inoculum of offer, sewage is jointly processed by 3 days.Through result index determining, the microbial flocculant is not only not
Can processing of other bacterial strains to sewage in influence system, moreover it is possible to improve the degraded situation of part index number, wherein COD degradation rate improves
9-14%, ammonia nitrogen degradation rate improves 12-16%.Solid suspended particle after its sewage disposal is determined by dry weight method, added with this
The control group that the experimental group of microbial flocculant does not add, its solid suspended particle content reduce more than 60%.Its sense organ is wadded a quilt with cotton
Solidifying effect is as shown in Figure 5.
Embodiment 2
A kind of preparation method of microbial flocculant, comprises the following steps：By Alteromonad（The screening technique of Alteromonad
It is same as Example 1）It is seeded in fermentation medium, shaking table culture, shaking speed 220rpm/min, cultivation temperature is 37 DEG C,
After cultivating 16h, after zymotic fluid is directly centrifuged, flocculence extracellular products is obtained, produces biological flocculant.The Alteromonas
Microbial flocculant caused by bacterium, the compound extracellular products of polysaccharide and albumen for the bacterium, its available direct visual perception, it is in
White flock, liquid bottom is settled down to, can directly centrifuge and obtain described microbial flocculant.
The composition of fermentation medium is：Peptone 5g/L, beef extract 5g/L, sodium chloride 2g/L, surplus are water, pH 7.0.
Embodiment 3
A kind of preparation method of microbial flocculant, comprises the following steps：By Alteromonad（The screening technique of Alteromonad
It is same as Example 1）It is seeded in fermentation medium, shaking table culture, shaking speed 220rpm/min, cultivation temperature is 37 DEG C,
After cultivating 20h, after zymotic fluid is directly centrifuged, flocculence extracellular products is obtained, produces biological flocculant.The Alteromonas
Microbial flocculant caused by bacterium, the compound extracellular products of polysaccharide and albumen for the bacterium, its available direct visual perception, it is in
White flock, liquid bottom is settled down to, can directly centrifuge and obtain described microbial flocculant.
The composition of fermentation medium is：Peptone 10g/L, beef extract 3g/L, sodium chloride 4g/L, surplus are water, and pH is
8.0。
Embodiment 4
A kind of preparation method of microbial flocculant, comprises the following steps：By Alteromonad（The screening technique of Alteromonad
It is same as Example 1）It is seeded in fermentation medium, shaking table culture, shaking speed 220rpm/min, cultivation temperature is 37 DEG C,
After culture 16 to 20h, after zymotic fluid is directly centrifuged, flocculence extracellular products is obtained, produces biological flocculant.The alternating
Microbial flocculant caused by monad, the compound extracellular products of polysaccharide and albumen for the bacterium, it with the naked eye can directly see
Examine, it is white cotton-shaped, liquid bottom is settled down to, can directly centrifuge and obtain described microbial flocculant.
Preferably, the composition of the fermentation medium is：Peptone 8g/L, beef extract 3.5g/L, sodium chloride 2.4g/L are remaining
Measure as water, pH 7.4.
Embodiment 5
Present invention also offers the preparation method of described complex biological flocculant, comprise the following steps：
（1）By Alteromonad（The screening technique of Alteromonad is same as Example 1）It is seeded in fermentation medium, shaking table
Culture, shaking speed 220rpm/min, cultivation temperature are 37 DEG C, after cultivating 16h, after zymotic fluid is directly centrifuged, are obtained
Flocculence extracellular products, produces biological flocculant；
（2）The preparation of complex biological flocculant：Flocculence extracellular products samples 0.5mL, and mass concentration is 1% copper ion solution
Dosage is 3mL, the processing for 1L water samples.
The composition of fermentation medium is：Peptone 10g/L, beef extract 3g/L, sodium chloride 2g/L, surplus are water, and pH is
7.3。
Embodiment 6
Present invention also offers the preparation method of described complex biological flocculant, comprise the following steps：
（1）By Alteromonad（The screening technique of Alteromonad is same as Example 1）It is seeded in fermentation medium, shaking table
Culture, shaking speed 220rpm/min, cultivation temperature are 37 DEG C, after cultivating 18h, after zymotic fluid is directly centrifuged, are obtained
Flocculence extracellular products, produces biological flocculant；
（2）The preparation of complex biological flocculant：Flocculence extracellular products samples 0.8mL, and mass concentration is 1% copper ion solution
Dosage is 2mL, the processing for 1L water samples.
The composition of fermentation medium is：Peptone 7g/L, beef extract 4g/L, sodium chloride 3.5g/L, surplus are water, and pH is
7.6。
Embodiment 7
Present invention also offers the preparation method of described complex biological flocculant, comprise the following steps：
（1）By Alteromonad（The screening technique of Alteromonad is same as Example 1）It is seeded in fermentation medium, shaking table
Culture, shaking speed 220rpm/min, cultivation temperature are 37 DEG C, after cultivating 17h, after zymotic fluid is directly centrifuged, are obtained
Flocculence extracellular products, produces biological flocculant；
（2）The preparation of complex biological flocculant：Flocculence extracellular products samples 0.8mL, and mass concentration is 1% copper ion solution
Dosage is 1.8mL, the processing for 1L water samples.
The composition of fermentation medium is：Peptone 8g/L, beef extract 4g/L, sodium chloride 2.6g/L, surplus are water, and pH is
7.7。
Claims (7)
1. a kind of Alteromonad, it is characterized in that：Alteromonad (Alteromonas sp.) G1-3, is deposited in China
General Microbiological Culture preservation administrative center, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, and preservation date is
On July 25th, 2017, deposit number are CGMCC No.14457.
2. biological flocculant prepared by Alteromonad according to claim 1, it is characterised in that the biological flocculant
The flocculence extracellular products obtained after directly being centrifuged by the tunning of the Alteromonad.
3. complex biological flocculant prepared by Alteromonad according to claim 1, it is characterised in that the compounding life
Thing flocculant includes biological flocculant and the solution of copper ions；The biological flocculant is produced by the fermentation of the Alteromonad
The flocculence extracellular products obtained after thing directly centrifugation；The copper ions solution compounding before mass concentration be 1% copper from
Sub- solution.
4. the preparation method of biological flocculant according to claim 2, it is characterized in that, comprise the following steps：By the friendship
It is seeded to for monad in fermentation medium, shaking table culture, shaking speed 220rpm/min, cultivation temperature is 37 DEG C, culture 16
To 20h, after zymotic fluid is directly centrifuged, flocculence extracellular products is obtained, produces biological flocculant.
5. the preparation method of biological flocculant according to claim 4, it is characterized in that, the composition of the fermentation medium
For：5-10g/L of peptone, 3-5g/L of beef extract, 2-4g/L of sodium chloride, surplus are water, and pH is 7.0-8.0.
6. the preparation method of complex biological flocculant according to claim 3, it is characterized in that, comprise the following steps：
（1）The Alteromonad is seeded in fermentation medium, shaking table culture, shaking speed 220rpm/min, culture temperature
Spend for 37 DEG C, after culture 16 to 20h, after zymotic fluid is directly centrifuged, obtain flocculence extracellular products, produce bioflocculation
Agent；
（2）The preparation of complex biological flocculant：Flocculence extracellular products samples 0.5-1mL, and the copper ion that mass concentration is 1% is molten
Liquid dosage is 1-3mL, the processing for 1L water samples.
7. the preparation method of complex biological flocculant according to claim 6, it is characterized in that, the group of the fermentation medium
Turn into：5-10g/L of peptone, 3-5g/L of beef extract, 2-4g/L of sodium chloride, surplus are water, and pH is 7.0-8.0.
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| CN111057667A (en) * | 2019-12-24 | 2020-04-24 | 天津科技大学 | Complex microbial inoculant and preparation method and application thereof |
| CN112795516A (en) * | 2021-02-07 | 2021-05-14 | 天津科技大学 | Complex microbial inoculant for decomposing and fermenting chicken manure compost |
| CN117446886A (en) * | 2023-12-26 | 2024-01-26 | 天津市好曰子动物药业有限公司 | Water quality improver for aquatic products and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111057667A (en) * | 2019-12-24 | 2020-04-24 | 天津科技大学 | Complex microbial inoculant and preparation method and application thereof |
| CN112795516A (en) * | 2021-02-07 | 2021-05-14 | 天津科技大学 | Complex microbial inoculant for decomposing and fermenting chicken manure compost |
| CN117446886A (en) * | 2023-12-26 | 2024-01-26 | 天津市好曰子动物药业有限公司 | Water quality improver for aquatic products and preparation method thereof |
| CN117446886B (en) * | 2023-12-26 | 2024-03-19 | 天津市好曰子动物药业有限公司 | Water quality improver for aquatic products and preparation method thereof |
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Application publication date: 20180223 |