CN105254020B - Method for quickly separating algae liquid in wastewater of microalgae treatment - Google Patents
Method for quickly separating algae liquid in wastewater of microalgae treatment Download PDFInfo
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- CN105254020B CN105254020B CN201510790371.9A CN201510790371A CN105254020B CN 105254020 B CN105254020 B CN 105254020B CN 201510790371 A CN201510790371 A CN 201510790371A CN 105254020 B CN105254020 B CN 105254020B
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- chlorella
- microalgae
- algae
- wastewater
- waste water
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- 241000195493 Cryptophyta Species 0.000 title claims abstract description 32
- 239000002351 wastewater Substances 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 title claims abstract description 9
- 238000012545 processing Methods 0.000 claims abstract description 11
- 238000004062 sedimentation Methods 0.000 claims abstract description 8
- 239000010865 sewage Substances 0.000 claims abstract description 7
- 230000003311 flocculating effect Effects 0.000 claims abstract description 4
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 28
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 229910052757 nitrogen Inorganic materials 0.000 claims description 11
- 238000002474 experimental method Methods 0.000 claims description 10
- 238000012360 testing method Methods 0.000 claims description 9
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 8
- 229910052698 phosphorus Inorganic materials 0.000 claims description 8
- 239000011574 phosphorus Substances 0.000 claims description 8
- 230000003068 static effect Effects 0.000 claims description 8
- 238000002798 spectrophotometry method Methods 0.000 claims description 6
- 238000004458 analytical method Methods 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 5
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 claims description 5
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 239000003344 environmental pollutant Substances 0.000 claims description 4
- 230000029052 metamorphosis Effects 0.000 claims description 4
- 238000007254 oxidation reaction Methods 0.000 claims description 4
- 231100000719 pollutant Toxicity 0.000 claims description 4
- 229920005479 Lucite® Polymers 0.000 claims description 3
- WYWFMUBFNXLFJK-UHFFFAOYSA-N [Mo].[Sb] Chemical compound [Mo].[Sb] WYWFMUBFNXLFJK-UHFFFAOYSA-N 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 230000000050 nutritive effect Effects 0.000 claims description 3
- 230000003647 oxidation Effects 0.000 claims description 3
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 3
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 238000004065 wastewater treatment Methods 0.000 claims description 3
- 241000192710 Microcystis aeruginosa Species 0.000 claims 1
- 239000011324 bead Substances 0.000 claims 1
- 238000000926 separation method Methods 0.000 abstract description 11
- 238000005265 energy consumption Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 2
- 230000008859 change Effects 0.000 abstract description 2
- 238000004140 cleaning Methods 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 229910052760 oxygen Inorganic materials 0.000 abstract description 2
- 239000001301 oxygen Substances 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 239000000470 constituent Substances 0.000 abstract 2
- 239000008394 flocculating agent Substances 0.000 abstract 1
- 244000000010 microbial pathogen Species 0.000 abstract 1
- 241000192701 Microcystis Species 0.000 description 3
- 238000005189 flocculation Methods 0.000 description 3
- 230000016615 flocculation Effects 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005553 drilling Methods 0.000 description 1
- 239000002659 electrodeposit Substances 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a method for quickly separating algae liquid in wastewater treated by microalgae, which is characterized in that wastewater discharged from a sewage treatment plant is collected firstly and placed indoors for later use, and COD (chemical oxygen demand) of the wastewater is treatedCrThe method is carried out in a culture device capable of controlling disturbance intensity, is convenient for controlling temperature and light, improves the efficiency of microalgae for treating wastewater, does not need to add a flocculating agent under the disturbance condition, realizes the separation of algae and liquid by forming a flocculating constituent by the change of the surface property of microalgae cells, can fully utilize disturbance energy consumption, reduces energy consumption, improves efficiency cost, can inhibit pathogenic microorganisms in a culture medium by increasing the pH of the culture liquid in the treatment process, has better sedimentation rate of the generated flocculating constituent, improves the separation efficiency of the algae and liquid, is simple and convenient to operate, has easy control of culture conditions, simple structure of an experimental device, small size, light weight, strong practicability and strong repeatability, the process is simple, the processing is convenient, the manpower, material resources and financial resources are saved, the operation is flexible, and the cleaning is convenient.
Description
Technical field
The present invention relates to algae solution in a kind of method of algae solution separation, more particularly to a kind of processing waste water applied to microalgae is quick
The method of separation.
Background technology
With China's expanding economy, substantial amounts of sanitary sewage, trade effluent are discharged into river by sewage disposal device
In, National Chemical oxygen demand total emission volumn in 2014 is 22,950,000 tons, and ammonia nitrogen total emission volumn is 238.5 ten thousand tons.Discharged after processing
Even if the discharge standard that sewage reaches one-level A is still far above the class water standard of surface water V, to improve the ecological environment, improving water money
The recycling of source and waste need to carry out advanced treating to waste discharge.Substantial amounts of research shows that microalgae handles waste water and waste water
The resource of middle pollutant has broad prospects.
Algae solution separation is the bottleneck of microalgae processing waste water commercial application during microalgae processing waste water, studies have reported that algae
Liquid separation accounts for the 20-30% of algae processing waste water totle drilling cost[1].The current algae solution isolation technics of induction and conclusion mainly has[2-3]：Inorganic wadding
It is solidifying, composite electrolyte flocculation, composite flocculation, centrifugation technique, filtering, the methods of electrolytic floatage and electrodeposit；Some uses first
Pre-oxidation treatment, then flocculation sediment is carried out to microalgae.There is high energy consumption in these algae solution isolation technics, produce secondary pollution, be not easy
Operate and by extraneous factor（Such as：Nutrient solution pH）Influence, it is more difficult to the popularization and application in industrialization production, it is main in industry at present
Water is carried out to algae using centrifugation technique.It can be seen that algae solution separation is still to restrict the master of microalgae processing waste water technology popularization and application
Want difficult point.
[1] Molina G E, Belarbi E H, Acien Fernandez F G, et al. 2003.
Recovery of Microalgal Biomass and Metabolites: Process Options and Economics
[J]. Biotechnol. Adv., (20): 491−515。
[2] Uduman, N. and Y. Qi, et al. 2010. Dewatering of microalgal
cultures: a major bottleneck to algae-based fuels." Journal of renewable and
sustainable energy 2 (1): 012701-16。
[3] Hu Yuansheng, Liu Bin, Hao Xiao, wait flocculation separation/harvesting research in the microalgaes of 2015. processing sewage
Status and prospects [J] ACTA Scientiae Circumstantiaes, 35 (1): 12-29.
The content of the invention
It is an object of the invention to provide a kind of method of algae solution quick separating in processing waste water applied to microalgae, by right
The control of hydrodynamic intensity, realize that microalgae produces autoflocculation during waste water is handled, should to realize the method for algae solution separation
Method technique is simple, and energy consumption is low, and treatment effeciency is high, and easy to operate, land occupation area is few, and caused biomass is pollution-free, and
And it is practical, it is easy to utilize.
The present invention is realized by following steps：
Step 1：It is standby in first collection sewage treatment plant discharge waste water holding chamber, to the COD of waste waterCrUsing potassium bichromate
Method, TN use potassium persulfate oxidation ultraviolet spectrophotometry, and TP uses molybdenum-antimony anti-spectrophotometric method, and ammonia nitrogen is using receiving formula reagent light
Degree method, pH use glass electrode method, referring specifically to《Water and effluent monitoring analysis method（Fourth edition）》；
Step 2：Wastewater treatment is carried out in controllable hydrodynamic force lucite groove, controls different hydrodynamic gradients,
0cm/s（Static control group）, 5cm/s, 10cm/s, 15cm/s, 25cm/s and 35cm/s.Experiment temperature control 29 in illumination box
± 0.5 DEG C, control and carried out under 2500 ± 50lx of light aseptic condition, Light To Dark Ratio 14：10h, make in the chlorella for stablizing growth period
For algae kind, initial algae density 20 × 104cell/mL；
Step 3：Test cultures liquid pH value, it is in increased trend to obtain nutrient solution pH with the increase of incubation time, in 9-10
Between；After experiment operation 3 days, stop water body flow, be continuously shot using iPhone videos, record algae solution in each culture apparatus
Separation situation etc., pass through analysis chlorella floccule body size and the parameter such as sinking speed, algae solution separation rate of recording a video；
Step 4：Lower chlorella different in flow rate is mapped to the clearance of total nitrogen total phosphorus, referring to the drawings shown in 1.From accompanying drawing 1
As can be seen that between chlorella distinguishes 55 ~ 77% and 72 ~ 78% to the clearance of total nitrogen total phosphorus in hydrodynamic test group, it is high
The total nitrogen and the 42% of total tp removal rate and 59% in static control group, flow velocity remove nutritive salt to chlorella and a suitable stream be present
Speed.
Step 5：Experiment obtains chlorella floccule body between 100 μm ~ 2000 μm, and sinking speed is in 1.6-3.2 × 10-3m/
Between s, flow velocity has optimal removal pollutant and microalgae flocculating setting effect between 25cm/s-35cm/s.It is by flow velocity
Mapped during 25cm/s after microcystis metamorphosis and sedimentation, shown in reference picture 2.From accompanying drawing 2 it can be seen that chlorella is in water
Colony is formed under dynamic action；And sedimentation is produced after water body is static, culture apparatus bottom is deposited to, chlorella group is calculated
The average sinking rate of body is in 0.42cm/s or so, it is seen that chlorella can be utilized itself to form colony under hydrodynamism real
Existing algae solution separation.The experiment parameter can meet that microalgae handles waste water commercial Application.
The solution have the advantages that：The present invention is carried out in the culture apparatus of controllable strength of turbulence, is easy to temperature control, control
Light, the efficiency of microalgae processing waste water is improved, is carried out under disturbed conditions, without adding flocculant, by microalgae cell superficiality
The change of matter forms floccule body and realizes that algae solution separates, and can make full use of disturbance energy consumption, reduces energy expenditure, improves efficiency cost,
The nutrient solution pH increases during processing, can suppress pathogenic microbes in culture medium, and caused floccule body has preferable
The rate of settling, algae solution separative efficiency is improved, easy to operate, condition of culture is easily controlled, and its experimental provision is simple in construction, small-sized
Light, practical, repeatability is strong, and technique is simple, easy to process, saves human and material resources and financial resources, and operate flexibly, just
In cleaning.
Brief description of the drawings
Fig. 1 is the chlorella of the invention under hydrodynamism to Determination of Total Nitrogen in Waste Water and the clearance of total phosphorus.
Fig. 2 is present invention microcystis metamorphosis and pictorial diagram after sedimentation when flow velocity is 25cm/s.
Fig. 3 is culture apparatus structural representation of the present invention.
In figure, 1, speed control 2, connecting line 3, motor 4, overhead arm 5, culture tank 6, rotary shaft 7, rotation direction 8,
Light source 9, culture metal line.
Embodiment
Beneficial effect possessed by the present invention is described in detail below in conjunction with accompanying drawing 1,2,3, it is intended to helps reader more
The essence of the present invention is understood well, but implementation that can not be to the present invention and protection domain form any restriction.
1st, it is standby in first collection sewage treatment plant discharge waste water holding chamber, to the COD of waste waterCrUsing potassium dichromate method, TN
Using potassium persulfate oxidation ultraviolet spectrophotometry, TP uses molybdenum-antimony anti-spectrophotometric method, ammonia nitrogen using receiving formula reagent photometry,
PH uses glass electrode method, referring specifically to《Water and effluent monitoring analysis method（Fourth edition）》；
2nd, wastewater treatment is carried out in controllable hydrodynamic force lucite groove, controls different hydrodynamic gradients, 0cm/s
（Static control group）, 5cm/s, 10cm/s, 15cm/s, 25cm/s and 35cm/s.Experiment temperature control 29 ± 0.5 in illumination box
DEG C, carried out under 2500 ± 50lx of control light aseptic condition, Light To Dark Ratio 14：10h, in stablizing the chlorella in growth period as algae
Kind, initial algae density 20 × 104cell/mL；
3rd, test cultures liquid pH value, it is in increased trend to obtain nutrient solution pH with the increase of incubation time, between 9-10；
After experiment operation 3 days, stop water body flow, be continuously shot using iPhone videos, record algae solution in each culture apparatus and separate
Situation etc., pass through analysis chlorella floccule body size and the parameter such as sinking speed, algae solution separation rate of recording a video；
4th, lower chlorella different in flow rate is mapped to the clearance of total nitrogen total phosphorus, referring to the drawings shown in 1.Can be with from accompanying drawing 1
Find out, chlorella is between the clearance difference 55 ~ 77% and 72 ~ 78% of total nitrogen total phosphorus, being above quiet in hydrodynamic test group
Only total nitrogen and the 42% of total tp removal rate and 59% in control group, flow velocity remove nutritive salt to chlorella and a suitable flow rate be present.
5th, experiment obtains chlorella floccule body between 100 μm ~ 2000 μm, and sinking speed exists
1.6-3.2×10-3Between m/s, flow velocity has optimal removal pollutant and microalgae wadding between 25cm/s-35cm/s
Solidifying effect of settling.Mapped when by flow velocity being 25cm/s after microcystis metamorphosis and sedimentation, shown in reference picture 2.From accompanying drawing 2
It can be seen that chlorella forms colony under hydrodynamism；And sedimentation is produced after water body is static, it is deposited to culture apparatus bottom
Portion, the average sinking rate of chlorella colony is calculated in 0.42cm/s or so, it is seen that chlorella can be utilized in hydrodynamic force
The lower colony that itself formed of effect realizes that algae solution separates.The experiment parameter can meet that microalgae handles waste water commercial Application.
Embodiment described above is only that the preferred embodiment of the present invention is described, not to the model of the present invention
Enclose and be defined, on the premise of design spirit of the present invention is not departed from, technical side of the those of ordinary skill in the art to the present invention
The various modifications and improvement that case is made, it all should fall into the protection domain of claims of the present invention determination.
Claims (1)
1. a kind of method of algae solution quick separating in processing waste water applied to microalgae, described microalgae is chlorella, is specifically included
Following steps：
（1）It is standby in the waste water holding chamber of first collection sewage treatment plant discharge, COD is surveyed using potassium dichromate method to waste waterCr, use
Potassium persulfate oxidation ultraviolet spectrophotometry surveyed total nitrogen, total phosphorus surveyed using molybdenum-antimony anti-spectrophotometric method, using receiving formula reagent luminosity
Method surveys ammonia nitrogen, surveys pH using glass electrode method；
（2）Wastewater treatment is carried out in controllable hydrodynamic force lucite groove, controls different hydrodynamic gradients, and 0cm/s is
Static control group, 5cm/s, 10cm/s, 15cm/s, 25cm/s and 35cm/s are test group, test the temperature control in illumination box
29 ± 0.5 DEG C, control and carried out under 2500 ± 50lx of light aseptic condition, Light To Dark Ratio 14:10h, in the chlorella for stablizing growth period
As algae kind, initial algae density 20 × 104cell/mL；
（3）Test cultures liquid pH value, it is in increased trend to obtain nutrient solution pH with the increase of incubation time, between 9 ~ 10；It is real
After testing operation 3 days, stop water body flow, be continuously shot using iPhone videos, record algae solution in each culture apparatus and separate feelings
Condition, pass through analysis chlorella floccule body size, settling height and the floccule body distributed constant of recording a video；
（4）Lower chlorella different in flow rate is studied the clearance of total nitrogen total phosphorus, draws the bead in hydrodynamic test group
Algae is above total nitrogen and total tp removal rate in static control group to the clearance of total nitrogen and total phosphorus difference 55 ~ 77% and 72 ~ 78%
42% and 59%, flow velocity removes nutritive salt to chlorella and a suitable flow rate be present；
（5）Experiment obtains chlorella floccule body in 100 μm ~ 2000 μm, sinking speed 1.6 ~ 3.2 × 10-3M/s, flow velocity exist
There is optimal removal pollutant and microalgae flocculating setting effect during 25cm/s ~ 35cm/s；Microcystis aeruginosa is thin when by flow velocity being 25cm/s
Studied after born of the same parents' metamorphosis and sedimentation, show that chlorella forms colony under hydrodynamism；And produced after water body is static
Raw sedimentation, is deposited to culture apparatus bottom, the average sinking rate of chlorella colony is calculated in 0.42cm/s；It can be seen that can
Colony itself being formed under hydrodynamism using chlorella and realizing that algae solution separates, the experiment parameter can meet that microalgae handles waste water
Commercial Application.
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| CN108640373B (en) * | 2018-05-03 | 2021-10-01 | 江西师范大学 | Method for promoting rapid separation of algae liquid in microalgae wastewater treatment by using calamus water extract |
| CN110008632B (en) * | 2019-04-18 | 2023-04-18 | 江西师范大学 | Method for rapidly calculating emergency pollution event of river channel type drinking water source |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101555455A (en) * | 2009-05-22 | 2009-10-14 | 新奥科技发展有限公司 | Culture/harvesting method of microalgae and system |
| CN101643700A (en) * | 2009-08-28 | 2010-02-10 | 刘永平 | Algae growth system device with two-step photobiologic reactors |
| CN103184158A (en) * | 2011-12-30 | 2013-07-03 | 新奥科技发展有限公司 | Microalgae collection method and application |
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2015
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101555455A (en) * | 2009-05-22 | 2009-10-14 | 新奥科技发展有限公司 | Culture/harvesting method of microalgae and system |
| CN101643700A (en) * | 2009-08-28 | 2010-02-10 | 刘永平 | Algae growth system device with two-step photobiologic reactors |
| CN103184158A (en) * | 2011-12-30 | 2013-07-03 | 新奥科技发展有限公司 | Microalgae collection method and application |
Non-Patent Citations (4)
| Title |
|---|
| pH值对淡水藻类生长和种类变化的影响;刘春光 等;《农业环境科学学报》;20051231;第24卷(第2期);第294-298页 * |
| 水动力条件下藻类动态模拟;丁玲 等;《生态学报》;20050830;第25卷(第8期);第1864-1868页 * |
| 流动条件下四尾栅藻对铜绿微囊藻生长的影响;李林 等;《重庆大学学报》;20130731;第36卷(第7期);第121-126页 * |
| 用流体动力技术治理蓝藻水华的研究;吴张永 等;《机床与液压》;20031231(第2期);第186页第1段-第187页最后一段及图1-3、表格 * |
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