CN107712870A - Nutritious food and preparation method thereof - Google Patents

Nutritious food and preparation method thereof Download PDF

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Publication number
CN107712870A
CN107712870A CN201710903233.6A CN201710903233A CN107712870A CN 107712870 A CN107712870 A CN 107712870A CN 201710903233 A CN201710903233 A CN 201710903233A CN 107712870 A CN107712870 A CN 107712870A
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nutritious food
rice
powder
food
semen
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王俊文
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/05—Mashed or comminuted pulses or legumes; Products made therefrom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/01—Instant products; Powders; Flakes; Granules
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/10—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/10—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
    • A23L19/115—Konjak; Konntaku
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L25/00—Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof
    • A23L25/30—Mashed or comminuted products, e.g. pulp, pastes, meal, powders; Products made therefrom, e.g. blocks, flakes, snacks; Liquid or semi-liquid products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products
    • A23L7/152—Cereal germ products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The present invention relates to a kind of nutritious food and preparation method thereof, belong to technical field of health care food.The nutritious food provided by the invention is to utilize the biased characteristic in terms of food (paddy flesh fruit dish) nature and flavor, by 50 kinds of raw material innovative combinations of " five cereals " and " integration of drinking and medicinal herbs " Chinese medicine, it is manufactured that there is natural, health, low in calories, the low glycemic nutritious food of safety, treatment or auxiliary treatment for disease, adjustment negative and positive are allowed to tend to balance, contain various nutriments needed by human, make up the continuous consumption of yang blood and qi, there is health care, auxiliary treatment, without any side effects, conditioning and treating effect slowly persistently, can be long-term use of.Present invention also offers the preparation method of nutritious food, and it is integrated with traditional technique and modern Chinese herbal medicine cell wall breaking technology, squeezing, maturing manufacturing technology, and obtained nutritious food is efficiently used for diseases prevention, cured the disease, the effect of health care.

Description

Nutritious food and preparation method thereof
Technical field
The invention belongs to technical field of health care food, and in particular to one kind is applied to healthy population or sub-health population, special It is not that middle-aged and old deficiency of vital energy negative and positive are gradually weak, each portion's underactivity of body, long-term chronic disease, nurses one's health biased constitution and health Nutritious food of health care and preparation method thereof.
Background technology
Announced according to World Health Organization 2007 one global, and the survey showed that, and mankind's sub-health population accounts for always The 75% of number, patient account for 20%, and healthy population accounts for 5%.Sub-health population is most of caused by intake nutrition is unbalanced, gives people Class body brings huge injury.Inferior health is ill omen, and the incubation period of pathogen.Inferior health is brought perhaps to the mankind More negative impacts, poor life quality, fatiguability, operating efficiency is low, and decrease of memory, appearance is bad, no creativity, easily transformation For chronic.Such as modern white collar clan, work, life multiple pressure are born, have ignored necessary maintenance, suffers from such as high blood Pressure, high fat of blood, hyperglycaemia etc. " modern trend disease ".
Name doctor's Zhang Xichun exists《Records of Tradition Chinese and Western Medicine in Combination》In described " dietotherapy clothes for patients is not only treated disease and can simultaneously allayed one's hunger, not only Allay one's hunger and agreeable to the taste can more be suited the medicine to the illness with it, disease is not suited the medicine to the illness from being gradually cured, also suffer from without him ".Therefore, regimen accommodation is wide, Mainly for sub-health population, next is only patient, as medicine or the supplementary means of other treatment measure, with diet Life is received naturally.So if can develop a kind of normal person take can provide grow and health existence needed for Various nutrients, physical fitness, clothes for patients it, not only treat disease, and the nutritious food that can be allayed one's hunger, in sub-health state Just seem for crowd most important.
The content of the invention
It is an object of the invention to provide one kind to be applied to healthy population or sub-health population, and particularly middle-aged and old deficiency of vital energys are cloudy Sun is gradually weak, each portion's underactivity of body, long-term chronic disease, nurses one's health the nutritious food and its system of biased constitution and health care Preparation Method, the nutritious food are made up of " five cereals " and the Chinese medicine of " integration of drinking and medicinal herbs ", and the nutritious food has natural, health, safety Low in calories, low glycemic, treatment or auxiliary treatment for some diseases, adjustment negative and positive be allowed to tend to balance, must containing human body The various nutriments needed, make up the continuous consumption of yang blood and qi.
To achieve these goals, technical scheme is specific as follows：
A kind of nutritious food, its raw material matched by following masses form：
Ginseng 5.0g, Cordyceps militaris 3.0g, sealwort 10.0g, fructus alpiniae oxyphyllae 5.0g, Chinese cassia tree 1.0g, Poria cocos 20.0g, Gorgon fruit 270.0g, Semen sesami nigrum 450.0g, Chinese yam rhizome 200.0g, black soya bean 200.0g, coix seed 50.0g, juglandis,semen 70.0g, matrimony vine 150.0g, bar denier reach wooden benevolence 50.0g, jujube 100.0g, hawthorn 2.0g, konjaku 35.0g, peanut 5.0g, mulberries 30.0g, lotus rhizome 1.0g, raspberry 50.0g, lily 1.0g, radix polygonati officinalis 1.0g, maca 1.0g, brown rice 5.0g, selenium-rich millet 30.0g, rice 5.0g, Glutinous rice 5.0g, purple rice 5.0g, oat 10.0g, whole wheat 5.0g, corn 20.0g, cowpea 30.0g, Semen Lablab Album 1.0g, lotus seeds 3.0g, Broad bean 1.0g, pawpaw 50.0g, wheat embryo 10.0g, mulberry leaf 1.0g, balsam pear 1.0g, celery 1.0g, peach powder 1.0g, rhizoma zingiberis 1.0g, black fungus powder 5.0g, white fungus 4.0g, banana 5.0g, blueberry powder 50.0g, Kiwi berry 10.0g, chrysanthemum 1.0g, salt 30g.
A kind of preparation method of nutritious food, comprises the following steps：
Step 1, according to quality proportioning weigh each raw material；
Step 2, by the ginseng weighed, Cordyceps militaris, sealwort, fructus alpiniae oxyphyllae, Chinese cassia tree, Poria cocos, yam and hawthorn, lily and jade Bamboo is cleared up, and 300 mesh fine powders are ground into broken wall disintegrating machine after drying；
Step 3, by the Gorgon fruit weighed, Semen sesami nigrum, black soya bean, coix seed, juglandis,semen, matrimony vine, bar denier up to wooden benevolence, jujube, evil spirit Taro, peanut, mulberries, lotus rhizome, raspberry, maca, brown rice, selenium-rich millet, rice, glutinous rice, purple rice, oat, whole wheat, corn, cowpea Beans, Semen Lablab Album, lotus seeds, broad bean, pawpaw, wheat embryo, mulberry leaf, balsam pear, celery, peach powder, rhizoma zingiberis, black fungus powder, white fungus, perfume (or spice) Any of several broadleaf plants, blueberry powder, Kiwi berry and chrysanthemum；Cleared up, mixed after drying, squeezing, maturing, smashed；
Step 4, the mixing fine powders that step 2 is obtained, the mixed powder that step 3 obtains and the salt that weighs are allocated, mixed It is even, pelleting, sterilizing, it is distributed into bag and can obtain the nutritious food.
The nutritious food shape of gained：It is powdered；Mouthfeel：Delicate fragrance slightly point is salty.Color：Light gray or somber grey；Specification： 20g/ bags.
Eating method：Sooner or later respectively take after mixing it with water once, 1 bag every time, continuous edible tri month, patient can treat illness, normally People can be with physical fitness.
The beneficial effects of the invention are as follows：
1st, the nutritious food provided by the invention is using the biased characteristic in terms of food (paddy flesh fruit dish) nature and flavor, by " five cereals " " integration of drinking and medicinal herbs " traditional Chinese medicinal material raw materials innovative combination, low in calories, the low glycemic nutritious food with natural, health, safety is made, Treatment or auxiliary treatment for disease, adjustment negative and positive are allowed to tend to balance, containing various nutriments needed by human, made up The continuous consumption of yang blood and qi, has the function that health care, auxiliary treatment, and without any side effects, conditioning and treating effect is slowly held Long, can be long-term use of.
2nd, nutritious food provided by the invention is applied to healthy population or sub-health population, particularly middle-aged and old deficiency of vital energy negative and positive It is gradually weak, each portion's underactivity of body, long-term chronic disease, nurse one's health biased constitution and health care.Institute in the nutritious food " integration of drinking and medicinal herbs " traditional Chinese medicinal material raw materials make the more preferable of its respective efficacy exertion by mutually acting synergistically, then with five cereal Matter combines, the various cardiotrophin proteins matter that make to want containing needed by human body in nutritious food, fat, carbohydrate, vitamin, mineral matter, water With all kinds of materials of cellulose (referring to table 1), you can cure the disease, equally can also serve as diet, life battalion is provided to body Foster replenishers, can be long-term use of.The conditioning such as this nutritious food dietotherapy kidney tonifying, invigorating the spleen, soothing the liver nourishment maintenance, dietotherapy reach rigidity of the body It is strong, early ageing is prevented and treated, has the function that foster skin, beauty, antifatigue, increasing intelligence, premature senescence resistance, blood-activating kidney-tonifying salubrity.
3rd, the inner wrapping conveniently taken is made in nutritious food provided by the invention, improves the powder preparation formulation of allusion side, Dan Pin Packaging, breach the limitation of herbal cuisine culinary art.The nutritious food is instant food, is easy to carry, convenient and instant, not by condition, environment Limitation, be adapted to modern fast pace life, for adhere to for a long time dietotherapy provide it is feasible take method, be more convenient to incorporate life habit It is used.
4th, the preparation method of nutritious food provided by the invention, traditional technique and modern Chinese herbal medicine breaking-wall cell skill are integrated with Art, squeezing, maturing manufacturing technology, obtained nutritious food are efficiently used for diseases prevention, cured the disease, the effect of health care.
Embodiment
Nutritious food provided by the invention, its raw material matched by following masses form：
Ginseng 5.0g, Cordyceps militaris 3.0g, sealwort 10.0g, fructus alpiniae oxyphyllae 5.0g, Chinese cassia tree 1.0g, Poria cocos 20.0g, Gorgon fruit 270.0g, Semen sesami nigrum 450.0g, Chinese yam rhizome 200.0g, black soya bean 200.0g, coix seed 50.0g, juglandis,semen 70.0g, matrimony vine 150.0g, bar denier reach wooden benevolence 50.0g, jujube 100.0g, hawthorn 2.0g, konjaku 35.0g, peanut 5.0g, mulberries 30.0g, lotus rhizome 1.0g, raspberry 50.0g, lily 1.0g, radix polygonati officinalis 1.0g, maca 1.0g, brown rice 5.0g, selenium-rich millet 30.0g, rice 5.0g, Glutinous rice 5.0g, purple rice 5.0g, oat 10.0g, whole wheat 5.0g, corn 20.0g, cowpea 30.0g, Semen Lablab Album 1.0g, lotus seeds 3.0g, Broad bean 1.0g, pawpaw 50.0g, wheat embryo 10.0g, mulberry leaf 1.0g, balsam pear 1.0g, celery 1.0g, peach powder 1.0g, rhizoma zingiberis 1.0g, black fungus powder 5.0g, white fungus 4.0g, banana 5.0g, blueberry powder 50.0g, Kiwi berry 10.0g, chrysanthemum 1.0g, salt 30g.
Nutritious food provided by the invention passes through the mouse to hypoimmunity by changing material composition and each raw material dosage Tested, what is finally just determined can significantly improve the body function of mouse, promote mouse health, battalion without any side effects Support meal raw material prescription and its each raw material dosage.
The preparation method of the nutritious food comprises the following steps：
Step 1, according to quality proportioning weigh each raw material；
Step 2, by the ginseng weighed, Cordyceps militaris, sealwort, fructus alpiniae oxyphyllae, Chinese cassia tree, Poria cocos, yam and hawthorn, lily and jade Bamboo is cleared up, and 300 mesh fine powders are ground into broken wall disintegrating machine after drying；
Step 3, by the Gorgon fruit weighed, Semen sesami nigrum, black soya bean, coix seed, juglandis,semen, matrimony vine, bar denier up to wooden benevolence, jujube, evil spirit Taro, peanut, mulberries, lotus rhizome, raspberry, maca, brown rice, selenium-rich millet, rice, glutinous rice, purple rice, oat, whole wheat, corn, cowpea Beans, Semen Lablab Album, lotus seeds, broad bean, pawpaw, wheat embryo, mulberry leaf, balsam pear, celery, peach powder, rhizoma zingiberis, black fungus powder, white fungus, perfume (or spice) Any of several broadleaf plants, blueberry powder, Kiwi berry and chrysanthemum；Cleared up, mixed after drying, squeezing, maturing, smashed；
Step 4, the mixing fine powders that step 2 is obtained, the mixed powder that step 3 obtains and the salt that weighs are allocated, mixed It is even, pelleting, sterilizing, it is distributed into bag and can obtain the nutritious food.
Nutritious food provided by the invention, its nutritional ingredient see the table below 1：
Table 1
Project Per 100g NRV%
Energy 1617KJ 19%
Protein 13.9g 23%
Fat 18.5g 31%
Carbohydrate 35.3g 12%
Sodium 625mg 31%
Vitamin A 127μg RE 16%
Vitamin E 17.81mgα-TE 127%
Vitamin B1 0.28mg 20%
Vitamin B2 0.21mg 15%
Nicotinic acid 2.28mg 16%
Phosphorus 232mg 33%
Potassium 349mg 17%
Magnesium 124mg 41%
Calcium 234mg 29%
Iron 8.3mg 55%
Zinc 2.47mg 16%
Selenium 10.0μg 20%
Copper 0.84mg 56%
Manganese 4.87mg 162%
Embodiment 1
Prepare the nutritious food of following quality proportionings：
Ginseng 5.0g, Cordyceps militaris 3.0g, sealwort 10.0g, fructus alpiniae oxyphyllae 5.0g, Chinese cassia tree 1.0g, Poria cocos 20.0g, Gorgon fruit 270.0g, Semen sesami nigrum 450.0g, Chinese yam rhizome 200.0g, black soya bean 200.0g, coix seed 50.0g, juglandis,semen 70.0g, matrimony vine 150.0g, bar denier reach wooden benevolence 50.0g, jujube 100.0g, hawthorn 2.0g, konjaku 35.0g, peanut 5.0g, mulberries 30.0g, lotus rhizome 1.0g, raspberry 50.0g, lily 1.0g, radix polygonati officinalis 1.0g, maca 1.0g, brown rice 5.0g, selenium-rich millet 30.0g, rice 5.0g, Glutinous rice 5.0g, purple rice 5.0g, oat 10.0g, whole wheat 5.0g, corn 20.0g, cowpea 30.0g, Semen Lablab Album 1.0g, lotus seeds 3.0g, Broad bean 1.0g, pawpaw 50.0g, wheat embryo 10.0g, mulberry leaf 1.0g, balsam pear 1.0g, celery 1.0g, peach powder 1.0g, rhizoma zingiberis 1.0g, black fungus powder 5.0g, white fungus 4.0g, banana 5.0g, blueberry powder 50.0g, Kiwi berry 10.0g, chrysanthemum 1.0g, salt 30g.
Step 1, according to quality proportioning weigh each raw material；
Step 2, by the ginseng weighed, Cordyceps militaris, sealwort, fructus alpiniae oxyphyllae, Chinese cassia tree, Poria cocos, yam and hawthorn, lily and jade Bamboo is cleared up, and 300 mesh fine powders are ground into broken wall disintegrating machine after drying；
Step 3, by the Gorgon fruit weighed, Semen sesami nigrum, black soya bean, coix seed, juglandis,semen, matrimony vine, bar denier up to wooden benevolence, jujube, evil spirit Taro, peanut, mulberries, lotus rhizome, raspberry, maca, brown rice, selenium-rich millet, rice, glutinous rice, purple rice, oat, whole wheat, corn, cowpea Beans, Semen Lablab Album, lotus seeds, broad bean, pawpaw, wheat embryo, mulberry leaf, balsam pear, celery, peach powder, rhizoma zingiberis, black fungus powder, white fungus, perfume (or spice) Any of several broadleaf plants, blueberry powder, Kiwi berry and chrysanthemum；Cleared up, mixed after drying, squeezing, maturing, smashed；
Step 4, the mixing fine powders that step 2 is obtained, the mixed powder that step 3 obtains and the salt that weighs are allocated, mixed It is even, pelleting, sterilizing, it is distributed into bag, every bag of 20g, you can obtain the nutritious food.
Immunity test is as follows：
BALB/c mouse 20, intraperitoneal injection of cyclophosphamide solution, inject within first day, press by 120mg/kg dosage within the 3rd day 100mg/kg dosage is injected, and prepares immunosuppression mouse model.
Mouse is randomly divided into 2 groups, every group 10, is followed successively by blank control group, 1 group of experiment.Every group of mouse stomach phase is equal For 28 days, the daily gavage amount of every mouse was 300 μ L/ days.The sterile physiological salt of the mouse stomach 0.85% of blank control group Water, test the nutritious food that 1 group of mouse prepares according to 5g/kg dosage gavage embodiment 1.
1st, the measure of humoral immune function-serum hemolysin experiment
Reagent：20% hematocrit sheep red blood cell (SRBC) (SRBC) physiological saline cell suspension, 10%SRB suspensions, physiology salt Water, with physiological saline 1：The guinea pig serum of 10 dilutions, Dou Shi reagents (sodium acid carbonate 1.0g, potassium cyanide 0.05g, high-potassium ferricyanide 0.2g, distilled water 1000mL)；
In gavage the 28th day, per mouse, intraperitoneal injection 0.2mL 20% SRBC, successive administration 5 days, gave in for the last time 1h after medicine, blood is taken from mouse orbit vein, centrifuge serum, take 500 times of serum normal saline dilution, take dilute serum 1mL is put in test tube, is added 10%SRBC suspension 0.5mL, is put ice bath, adds with physiological saline 1：The guinea pig serum of 10 dilutions 1mL is mixed, and separately sets the blank control of not increase serum.After 37 DEG C of water bath with thermostatic control insulation 10min of dislocation, ice bath is moved immediately and is terminated instead Should.10min is centrifuged with 2000r/min, takes supernatant 1mL, adds Dou Shi reagent 3mL, mixes and places 10 minutes, take supernatant with 96 holes Plate colorimetric at scanning enzyme mark spectrophotometer 540nm, surveys absorbance (OD).Separately 10%SRBC suspensions 0.25mL is taken to add all Family name reagent 3.75mL, OD values are ibid determined, as half hemolytic value.
Serum hemolysis cellulose content is with half hemolytic value (HC50) represent.It is calculated as follows：HC50OD values/SRBC of=sample OD values × extension rate during half hemolysis.Experimental result is shown in Table 2.
Table 2
Group Dosage Serum hemolysin
Blank control group 0.85% physiological saline 160.59±7.86
Test 1 group 5g/kg 178.21±2.31a
A expressions are compared with blank control group, p ＜ 0.05；
The result of table 2 shows：In terms of mice serum hemolysin generation, compared with physiological saline blank control group, 1 group is tested With significant difference, it can more significantly increase the generation of serum hemolysin.
2nd, measure-lactic dehydrogenase (LDH) determination method of NK cytoactives
In 96 porocyte culture plates, effector cell (2 × 10 is added in experimental port7Individual/mL) and target cell (4 × 105Individual/ ML) (effect target ratio is 50 to each 100 μ L:1) target cell and each 100 μ L of nutrient solution, target cell maximum, are added in target cell Spontaneous release hole Target cell and each 100 μ L of 2.5%Triton, above-mentioned items are added to be all provided with three parallel holes in release aperture.In 37 DEG C, 5%CO2Culture 4h is cultivated in case；96 well culture plates are centrifuged into 10min with 1500r/min, the μ L of supernatant 100 are drawn per hole, while add LDH matrix The μ L of liquid 100, the HCl (1M) of 30 μ L of every hole addition after 10min is reacted, OD values are determined at ELIASA 570nm and are recorded.NK cells Activity calculates according to the following formula：
NK cytoactives (%)=[(test group OD values-Spontaneous release control group OD values)/(maximum release control group OD values- Spontaneous release control group OD values)] × 100%；Experimental result is shown in Table 3.
Table 3
Group Dosage NK cytoactives (%)
Blank control group 0.85% physiological saline 17.59±3.21
Test 1 group 5g/kg 37.04±1.10a
A expressions are compared with blank control group, p ＜ 0.05；
The result of table 3 shows：Compared with physiological saline blank control group, 1 group of experiment has to NK cells in mice killing activity Significantly humidification (p ＜ 0.05).
3rd, ConA inducing mouses Splenic vein hemodynamics are tested
It is sterile to each group mouse to take spleen, cell suspension is prepared, it is 3 to adjust splenocyte concentration with RPMI1640 complete culture solutions ×109Individual/L, lymphproliferation response is carried out according to MTr methods in program, is finally determined with ultraviolet-uisible spectrophotometer Extinction OD values at 570nm wavelength, carry out variance analysis.Experimental result is shown in Table 4.
Table 4
A expressions are compared with blank control group, p ＜ 0.05；
Table 4 test result indicates that：Compared with physiological saline blank control group, the Splenic vein hemodynamics of 1 group of mouse are tested Rate is significantly higher than the conversion ratio of the splenic lymphocytes of blank control group mouse.
Above-mentioned immunity test shows：Nutritious food provided by the invention is by by " five cereals " and " integration of drinking and medicinal herbs " traditional Chinese medicinal material raw materials Reasonably combined, Synergistic between each raw material, what is embodied is not simple Overlay in theory, but One function effect Qualitative leap.Nutritious food provided by the invention can significantly improve the body function of mouse, mouse is gradually recovered one's health, entirely In experimentation, without any adverse reaction.
Obviously, above-described embodiment is only intended to clearly illustrate example, and is not the restriction to embodiment.It is right For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or Among changing still in the protection domain of the invention.

Claims (2)

1. a kind of nutritious food, it is characterised in that its raw material matched by following masses forms：
It is ginseng 5.0g, Cordyceps militaris 3.0g, sealwort 10.0g, fructus alpiniae oxyphyllae 5.0g, Chinese cassia tree 1.0g, Poria cocos 20.0g, Gorgon fruit 270.0g, black Sesame 450.0g, Chinese yam rhizome 200.0g, black soya bean 200.0g, coix seed 50.0g, juglandis,semen 70.0g, matrimony vine 150.0g, Ba Danda Wooden benevolence 50.0g, jujube 100.0g, hawthorn 2.0g, konjaku 35.0g, peanut 5.0g, mulberries 30.0g, lotus rhizome 1.0g, raspberry 50.0g, lily 1.0g, radix polygonati officinalis 1.0g, maca 1.0g, brown rice 5.0g, selenium-rich millet 30.0g, rice 5.0g, glutinous rice 5.0g, purple Rice 5.0g, oat 10.0g, whole wheat 5.0g, corn 20.0g, cowpea 30.0g, Semen Lablab Album 1.0g, lotus seeds 3.0g, broad bean 1.0g, wood Melon 50.0g, wheat embryo 10.0g, mulberry leaf 1.0g, balsam pear 1.0g, celery 1.0g, peach powder 1.0g, rhizoma zingiberis 1.0g, black fungus powder 5.0g, white fungus 4.0g, banana 5.0g, blueberry powder 50.0g, Kiwi berry 10.0g, chrysanthemum 1.0g, salt 30g.
2. the preparation method of nutritious food according to claim 1, it is characterised in that comprise the following steps：
Step 1, according to quality proportioning weigh each raw material；
Step 2, the ginseng weighed, Cordyceps militaris, sealwort, fructus alpiniae oxyphyllae, Chinese cassia tree, Poria cocos, yam and hawthorn, lily and radix polygonati officinalis entered Row cleaning, 300 mesh fine powders are ground into after drying with broken wall disintegrating machine；
Step 3, by the Gorgon fruit weighed, Semen sesami nigrum, black soya bean, coix seed, juglandis,semen, matrimony vine, bar denier up to wooden benevolence, jujube, konjaku, flower It is life, mulberries, lotus rhizome, raspberry, maca, brown rice, selenium-rich millet, rice, glutinous rice, purple rice, oat, whole wheat, corn, cowpea, white French beans, lotus seeds, broad bean, pawpaw, wheat embryo, mulberry leaf, balsam pear, celery, peach powder, rhizoma zingiberis, black fungus powder, white fungus, banana, blueberry Powder, Kiwi berry and chrysanthemum；Cleared up, mixed after drying, squeezing, maturing, smashed；
Step 4, the mixing fine powders that step 2 is obtained, the mixed powder that step 3 obtains and the salt that weighs are allocated, mixed, and are made Particle, sterilizing, it is distributed into bag and can obtain the nutritious food.
CN201710903233.6A 2017-09-29 2017-09-29 Nutritious food and preparation method thereof Pending CN107712870A (en)

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