CN107712169A - A kind of tea Processing method - Google Patents
A kind of tea Processing method Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/18—Extraction of water soluble tea constituents
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Abstract
The present invention provides a kind of tea Processing method, comprises the following steps：Preextraction is carried out to tea raw material with 50~90% edible ethanols, obtains the first extract solution and tea grounds, tea grounds is then prepared into the high instant tea of content of tea polysaccharide using enzymolysis and water extraction mode；The macroporous absorbent resin and the mode that is combined of different eluents of different performance are recycled, collects the eluent in each stage respectively, the instant tea that processing and extracting obtains the high instant tea of the high instant tea of the high instant tea of ester catechin content, caffeine content, non-ester catechin content and theanine content is high.The present invention prepares the high functional form instant tea of multiple efficacies component content simultaneously by a technological process, effectively increases the utilization rate of tea raw material, while improve value-added content of product.
Description
Technical field
The present invention relates to field of deep tea processing technology, more particularly to a kind of tea Processing method.
Background technology
In recent years, tea comprehensive processing industry in China's develops rapidly, it is contemplated that there will be over 50% tealeaves as former to the year two thousand twenty
Material is applied to the industries such as food, medicine and health products by deep processing.It is but deep to add because China's tea comprehensive processing industry starts to walk evening
Work is backward in technique, and mainly extensive style increases.
At present, there is more deficiency in tea comprehensive processing, as tea comprehensive processing raw material is made a low multiple use, tea natural product
Added value is low, and functional components using acid-base method, organic solvent extractionprocess, precipitation by metallic ion method etc. carry in tealeaves
Take so that tealeaves extraction product is made and has that functional component purity is low, loss is big, organic solvent residual, functional component activity drop
Low deficiency, its application in fields such as food, medicine, health products is limited, and technique is not green enough, does not meet current
The development trend of tea industry.
Instant tea (Instant tea powder), maintains the original color of tealeaves, and have nutrition concurrently, defend substantially
It is raw, conveniently, it is graceful the features such as, more and more important role is play in deep processing field of tea.However, with society and economy
Development, people more focus on the security and health of diet, and instant tea industry development faces opportunities and challenges.It is but existing
Instant tea the effect of component content it is relatively low, limit its application in fields such as medicine, health products, cosmetics, this is to instant
Tea industry development proposes new challenge, and " feature " turns into further innovation target.
Therefore, it is necessary to invent a kind of tea that can be improved tea raw material utilization rate and improve functional component content in instant tea
Leaf processing method.
The content of the invention
It is an object of the invention to overcome drawbacks described above, there is provided one kind can improve tea raw material utilization rate and improve instant
The tea Processing method of functional component content in tea.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention is：
The present invention provides a kind of tea Processing method, comprises the following steps：
Step 1：Into tea raw material add 5~20 times volumetric concentration for 50~90% edible ethanols carry out extraction 20~
60min, repeat to extract, tea grounds is collected by filtration, merge filtered fluid and obtain the first extract solution；
Step 2：The enzyme and tea raw material 5 that weight is 0.5~5.0wt% of tea raw material are added into step 1 gained tea grounds
~20 times of water extracts 20~60min at a temperature of 30~60 DEG C, repeats to extract, filtering, merges filtered fluid and obtains the second extraction
Liquid, then membrane filtration is carried out to the second extract solution and obtains trapped fluid, trapped fluid is concentrated successively, dried, obtaining content of tea polysaccharide is
More than 20wt% instant tea, wherein, the enzyme is one or both of pectase and cellulase；
Step 3：First extract solution obtained by step 1 is subjected to the supernatant after centrifugation removal of impurities and carries out concentration and recovery ethanol, is obtained
Aqueous phase concentrate, aqueous phase concentrate is obtained into clarified solution using the ceramic membrane filter that aperture is 0.2~0.5 μm, by the clarified solution
The first macroporous absorbent resin for being added to energy selective absorption ester catechin is adsorbed, then dense with 1.0~5.0BV volumes
The edible ethanol for 5~20% is spent as the first eluent, by 1.0~3.5BV/h elution flow rate to the first macroporous absorption tree
Fat is eluted, and is collected the first eluent and first and is crossed post liquid；Again with 1.0~6.0BV volumetric concentrations be 30~75% it is edible
Ethanol is eluted, Ran Houshou as the second eluent by 1.0~3.5BV/h elution flow rate to the first macroporous absorbent resin
Collect the second eluent, the aqueous phase substance after the second eluent concentration and recovery ethanol is dried, obtaining ester catechin content is
More than 50wt% instant tea；
Step 4：The first eluent in step 3 and first are crossed into post liquid and merge to obtain mixed liquor, the mixed liquor is added
The second macroporous absorbent resin to energy selective absorption caffeine is adsorbed, and the second macroporous absorbent resin is washed with water
It is de-, collect second and cross post liquid and water lotion, be then used as the by the use of the edible ethanol that 1.0~6.0BV volumetric concentrations are 10~50%
Three eluents, the second macroporous absorbent resin is eluted by 1.0~3.5BV/h elution flow rate, collect the 3rd eluent, will
Aqueous phase substance after 3rd eluent concentration and recovery ethanol is dried, and obtains the instant tea that caffeine content is more than 20wt%；
Post liquid is crossed by second and water lotion mixes, and is then filtered, is then carried out obtained filtered fluid instead with reverse osmosis membrane
Infiltration is handled, and collects reverse osmosis concentrated liquid and counter-infiltration permeate, by the reverse osmosis concentrated liquid it is dry non-ester catechin
Content is more than 40wt% instant tea；
Step 5：Counter-infiltration permeate in step 4 is added to the ion exchange resin for being capable of selective absorption theanine
Adsorbed, then by the use of the sodium hydroxide solution that 0.5~5.0BV molar concentrations are 0.01~0.2mol/L as the 4th eluent,
Ion exchange resin is eluted by 1.0~3.5BV/h elution flow rate, then collects the 4th eluent, by the 4th elution
Aqueous phase substance after liquid concentration and recovery ethanol is dried, and obtains the instant tea that theanine content is more than 15wt%.
The beneficial effects of the present invention are：(1) present invention is prepared content of tea polysaccharide and is simultaneously by a technological process
Instant tea that more than 20wt% instant tea, ester catechin content are more than 50wt%, caffeine content are more than 20wt%
Instant tea, non-ester catechin content be more than 40wt% instant tea and theanine content be the instant of more than 15wt%
Tea, effectively increases the utilization rate of tea raw material, while improves value-added content of product；(2) in the method that the present invention uses not
Using poisonous and harmful reagent, a kind of organic solvent of edible ethanol is related generally to, method is more green, safe operation, also protects
The safety of product has been demonstrate,proved, has all been had broad application prospects in functional food, cakes and sweetmeats product, Medicines and Health Product and cosmetic field.
Embodiment
To describe the technology contents of the present invention, construction feature, the objects and the effects in detail, below in conjunction with embodiment
It is explained in detail.
The design of most critical of the present invention is：The extracting mode combined by using water extraction with alcohol extracting, be re-introduced into zymotechnic,
UF membrane and macroporous absorbent resin technology, comprehensive process is carried out to tealeaves, to improve the utilization rate of tea raw material, prepared simultaneously
The high instant tea of multiple efficacies component content.
A kind of tea Processing method, comprises the following steps：
Step 1：Into tea raw material add 5~20 times volumetric concentration for 50~90% edible ethanols carry out extraction 20~
60min, repeat to extract, tea grounds is collected by filtration, merge filtered fluid and obtain the first extract solution；
Step 2：The enzyme and tea raw material 5 that weight is 0.5~5.0wt% of tea raw material are added into step 1 gained tea grounds
~20 times of water extracts 20~60min at a temperature of 30~60 DEG C, repeats to extract, filtering, merges filtered fluid and obtains the second extraction
Liquid, then membrane filtration is carried out to the second extract solution and obtains trapped fluid, trapped fluid is concentrated successively, dried, obtaining content of tea polysaccharide is
More than 20wt% instant tea, wherein, the enzyme is one or both of pectase and cellulase；
Step 3：First extract solution obtained by step 1 is subjected to the supernatant after centrifugation removal of impurities and carries out concentration and recovery ethanol, is obtained
Aqueous phase concentrate, aqueous phase concentrate is obtained into clarified solution using the ceramic membrane filter that aperture is 0.2~0.5 μm, by the clarified solution
The first macroporous absorbent resin for being added to energy selective absorption ester catechin is adsorbed, then dense with 1.0~5.0BV volumes
The edible ethanol for 5~20% is spent as the first eluent, by 1.0~3.5BV/h elution flow rate to the first macroporous absorption tree
Fat is eluted, and is collected the first eluent and first and is crossed post liquid；Again with 1.0~6.0BV volumetric concentrations be 30~75% it is edible
Ethanol is eluted, Ran Houshou as the second eluent by 1.0~3.5BV/h elution flow rate to the first macroporous absorbent resin
Collect the second eluent, the aqueous phase substance after the second eluent concentration and recovery ethanol is dried, obtaining ester catechin content is
More than 50wt% instant tea；
Step 4：The first eluent in step 3 and first are crossed into post liquid and merge to obtain mixed liquor, the mixed liquor is added
The second macroporous absorbent resin to energy selective absorption caffeine is adsorbed, and the second macroporous absorbent resin is washed with water
It is de-, collect second and cross post liquid and water lotion, be then used as the by the use of the edible ethanol that 1.0~6.0BV volumetric concentrations are 10~50%
Three eluents, the second macroporous absorbent resin is eluted by 1.0~3.5BV/h elution flow rate, collect the 3rd eluent, will
Aqueous phase substance after 3rd eluent concentration and recovery ethanol is dried, and obtains the instant tea that caffeine content is more than 20wt%；
Post liquid is crossed by second and water lotion mixes, and is then filtered, is then carried out obtained filtered fluid instead with reverse osmosis membrane
Infiltration is handled, and collects reverse osmosis concentrated liquid and counter-infiltration permeate, by the reverse osmosis concentrated liquid it is dry non-ester catechin
Content is more than 40wt% instant tea；
Step 5：Counter-infiltration permeate in step 4 is added to the ion exchange resin for being capable of selective absorption theanine
Adsorbed, then by the use of the sodium hydroxide solution that 0.5~5.0BV molar concentrations are 0.01~0.2mol/L as the 4th eluent,
Ion exchange resin is eluted by 1.0~3.5BV/h elution flow rate, then collects the 4th eluent, by the 4th elution
Aqueous phase substance after liquid concentration and recovery ethanol is dried, and obtains the instant tea that theanine content is more than 15wt%.
Wherein, the first macroporous absorbent resin described in step 3 produces for Xi'an Sunresin New Materials Co., Ltd.
LX-8 resins, the AB-8 resins and the limited public affairs of Cangzhou treasured grace sorbing material science and technology of the production of Tianjin Nankai Compositech Inc.
Any one resin taken charge of in the HPD100 resins of production；
The second macroporous absorbent resin described in step 4 is the LS-303 trees of Shaanxi Lan Shen Special Resins Co., Ltd production
The D4020 resins of fat, the DM-2 resins of Tianjin sea light Chemical Co., Ltd. production and the production of Shandong Shandong limited company
In any one；
Ion exchange resin described in step 5 for the production of Tianjin Nankai Compositech Inc. D113 faintly acids sun from
Sub-exchange resin, 732 cationic ion-exchange resins of Shanghai Huazhen Science and Technology Co., Ltd.'s production and Shanghai Huazhen Science and Technology Co., Ltd.
Any one in the D301 weak anion exchange resins of production.
Functional form instant tea is defined as, and using tealeaves, tea fresh leaves or instant tea as raw material, is extracted through water, ethanol extracts, tea is fresh
Leaf is squeezed the juice or instant tea weight is molten, then a certain function of tealeaves being processed into through the purifying of alcohol precipitation, enzymolysis, UF membrane or adsorption resin method
Composition or the solid product of the high enrichment of a kind of function ingredients, can be used as food, functional beverage, medicine, health products, daily cosmetic
The supplementary material of the field such as product product.
The present invention principle be：The present invention first passes through 50~90% edible ethanols and tea raw material is slightly carried, and is contained
There are the first extract solution and tea of multiple efficacies composition (containing ester catechin, non-ester catechin, caffeine and theanine)
Slag (contains tea polysaccharide), and tea grounds is digested followed by pectase and/or cellulase, while utilizes water extraction, so as to profit
The film for meeting retention tea polysaccharide molecule with pore size carries out membrane filtration, and trapped fluid is concentrated, dries obtained content of tea polysaccharide height
Instant tea；Then different efficacies composition otherness existing for solubility in different concentration ethanol in the first extract solution is utilized
And the macroporous absorbent resin with different adsorption functions, divided successively by certain elution flow rate using the edible ethanol of various concentrations
From and handle and prepare the high instant tea of ester catechin content and high instant tea (5~20% edible second of caffeine content
Alcohol is as the first eluent to remove the caffeine and non-ester catechin in the first polymeric adsorbent)；Due to non-ester catechin
Had differences with the molecular size range of theanine, recycle reverse osmosis membrane to carry out counter-infiltration to the post liquid of crossing after washing away caffeine
Processing, non-ester catechin can be intercepted in reverse osmosis concentrated liquid, and the small molecule such as theanine can pass through reverse osmosis membrane and occur
In counter-infiltration permeate, the high instant tea of non-ester catechin content is dried to obtain to reverse osmosis concentrated liquid；Recycle ion
Theanine in polymeric adsorbent absorption counter-infiltration permeate, and eluted using the suitable sodium hydroxide solution of molar concentration,
Processing obtains the high instant tea of theanine content.
It was found from foregoing description, the beneficial effects of the present invention are：(1) present invention is prepared simultaneously by a technological process
Go out instant tea, caffeine that instant tea, ester catechin content that content of tea polysaccharide is more than 20wt% are more than 50wt% to contain
Measuring the instant tea for more than 20wt%, the instant tea that non-ester catechin content is more than 40wt% and theanine content is
More than 15wt% instant tea, effectively increases the utilization rate of tea raw material, while improves value-added content of product；(2) this hair
Without using poisonous and harmful reagent in the method for bright use, a kind of organic solvent of edible ethanol, the more green ring of method are related generally to
Protect, safe operation, also ensure that the safety of product, all have in functional food, cakes and sweetmeats product, Medicines and Health Product and cosmetic field
Wide application prospect.
Further, the Extracting temperature in step 1 is 30~90 DEG C.
Seen from the above description, step 1 Extracting temperature control at 30~90 DEG C, can accelerate functional component in edible second
Dissolving in alcoholic solution, to improve extraction efficiency.
Further, first extract solution is to repeat extraction 2~3 times according to step 1, and tea grounds is collected by filtration, merged
Filtrate, obtain the first extract solution；Second extract solution is to repeat extraction 2~3 times according to step 2, filtering, merges filtered fluid,
Obtain the second extract solution.
Seen from the above description, first and second extract solution uses the mode for repeating to extract to improve carrying for both effectiveness composition
Take rate.
Further, the concrete operations of the membrane filtration in step 2 are：It it is first 0.2~0.5 μm with aperture by the second extract solution
Ceramic membrane filter, filtered fluid again with molecular cut off be 10~70KD milipore filter filtered, obtain trapped fluid.
Seen from the above description, preliminary filtering and impurity removing, clarification are carried out first with above-mentioned ceramic membrane, then carries out milipore filter mistake
Filter, with the purity accelerated the speed of milipore filter and improve the tea polysaccharide in instant tea.
Further, the adsorption flow rate adsorbed in step 3 is controlled in 0.5~2.0BV/h；The absorption stream adsorbed in step 4
Speed control is in 0.5~3.0BV/h；Absorption flow control in step 5 is in 1.0~3.5BV/h speed.
Seen from the above description, the adsorption flow rate in each step is controlled in above range respectively, is ensuring resin adsorption
The efficiency of adsorption treatment is taken into account while completely.
Further, the mode of the filtering in step 1 and step 2 is centrifugation, filter-cloth filtering, plate-frame filtering or vibration
It is sieved through any one in filter.
Further, the tea raw material is green tea, white tea or oolong tea.
Embodiment 1
A kind of tea Processing method, comprises the following steps：
Step 1：5 kilograms of large green tea (tea raw material) are weighed, are with deionized water or pure water dose volume concentration
65% edible ethanol, the volumetric concentration that 75 kilograms are added into 5 kilograms of large green tea is temperature of 65% edible ethanol at 55 DEG C
Under carry out extraction 30min, repeat extraction 2 times, tea grounds be collected by filtration, merge filtered fluid, obtain the first extract solution；
Step 2：7.5 grams of pectase (enzyme activity 10000u/g) and 25 kilograms of water is added into step 1 gained tea grounds
35min is extracted at a temperature of 45 DEG C, extraction 2 times is repeated, filtering, merges filtered fluid and obtain the second extract solution, be then with aperture
0.2~0.5 μm of ceramic membrane is filtered to the second extract solution, and the milipore filter that filtered fluid is again 30KD with molecular cut off is carried out
Filtering, trapped fluid is collected, trapped fluid is concentrated successively, is spray-dried, obtain the instant tea that content of tea polysaccharide is 35.62wt%
Powder；
Step 3：First extract solution obtained by step 1 is subjected to the supernatant after centrifugation removal of impurities and carries out concentration and recovery ethanol, is obtained
Aqueous phase concentrate, aqueous phase concentrate is obtained into clarified solution using the ceramic membrane filter that aperture is 0.2~0.5 μm, by the clarified solution
It is added to first macroporous absorbent resin (Xi'an Sunresin New Materials Co., Ltd. of energy selective absorption ester catechin
The LX-8 resins of production) adsorbed (adsorption flow rate 2.0BV/h), then with the edible second that 4.5BV volumetric concentrations are 10%
Alcohol is eluted to the first macroporous absorbent resin by 2.5BV/h elution flow rate as the first eluent, collects the first eluent
Post liquid is crossed with first；Again by the use of the edible ethanol that 4.0BV volumetric concentrations are 40% as the second eluent, by 2.0BV/h elution
Flow velocity elutes to the first macroporous absorbent resin, then collects the second eluent, after the second eluent concentration and recovery ethanol,
The aqueous phase substance that solid content is 15% is obtained, aqueous phase substance is spray-dried, obtaining ester catechin content is
72.63wt% instant tea powder；
Step 4：The first eluent in step 3 and first are crossed into post liquid and merge to obtain mixed liquor, the mixed liquor is added
To the second macroporous absorbent resin (LS-303 trees of Shaanxi Lan Shen Special Resins Co., Ltd production of energy selective absorption caffeine
Fat) (adsorption flow rate 2.0BV/h) is adsorbed, the second macroporous absorbent resin is eluted with water, second is collected and crosses post liquid
And water lotion, then by the use of the edible ethanol that 3.0BV volumetric concentrations are 20% as the 3rd eluent, by 2.5BV/h elution stream
Speed elutes to the second macroporous absorbent resin, the 3rd eluent is collected, by the aqueous phase after the 3rd eluent concentration and recovery ethanol
Material is spray-dried, and obtains the instant tea powder that caffeine content is 27.55wt%；
Post liquid is crossed by second and water lotion mixes, and is then filtered, is then carried out obtained filtered fluid instead with reverse osmosis membrane
Infiltration is handled, and collects reverse osmosis concentrated liquid and counter-infiltration permeate, the reverse osmosis concentrated liquid is carried out to be spray-dried non-ester
Type catechin content is 46.78wt% instant tea powder；
Step 5：Counter-infiltration permeate in step 4 is added to the ion exchange resin for being capable of selective absorption theanine
The D113 weak-acid cation-exchange resins of production (Tianjin Nankai Compositech Inc.) is adsorbed, and (adsorption flow rate is
3.5BV/h), then by the use of the sodium hydroxide solution that 3.0BV molar concentrations are 0.1mol/L as the 4th eluent, by 2.0BV/h's
Elution flow rate elutes to ion exchange resin, the 4th eluent is then collected, after the 4th eluent concentration and recovery ethanol
Aqueous phase substance be dried, obtain theanine content be 17.43wt% instant tea powder.
Embodiment 2
A kind of tea Processing method, comprises the following steps：
Step 1：5 kilograms of large oolong tea (tea raw material) are weighed, are with deionized water or pure water dose volume concentration
70% edible ethanol, the volumetric concentration that 75 kilograms are added into 5 kilograms of large green tea is temperature of 70% edible ethanol at 65 DEG C
Under carry out extraction 40min, repeat extraction 2 times, tea grounds be collected by filtration, merge filtered fluid, obtain the first extract solution；
Step 2：Add into step 1 gained tea grounds 10 grams cellulase (enzyme activity 100000u/g) and 50 kilograms
Water extracts 35min at a temperature of 40 DEG C, repeats extraction 2 times, filtering, merges filtered fluid and obtain the second extract solution, then use aperture
The second extract solution is filtered for 0.2~0.5 μm of ceramic membrane, the milipore filter that filtered fluid is again 50KD with molecular cut off enters
Row filtering, collects trapped fluid, trapped fluid is concentrated successively, is spray-dried, and it is the instant of 47.39wt% to obtain content of tea polysaccharide
Tea powder；
Step 3：First extract solution obtained by step 1 is subjected to the supernatant after centrifugation removal of impurities and carries out concentration and recovery ethanol, is obtained
Aqueous phase concentrate, aqueous phase concentrate is obtained into clarified solution using the ceramic membrane filter that aperture is 0.2~0.5 μm, by the clarified solution
Be added to can selective absorption ester catechin the first macroporous absorbent resin (Tianjin Nankai Compositech Inc. production
AB-8 resins) adsorbed (adsorption flow rate 2.0BV/h), then by the use of 4.0BV volumetric concentrations be 15% edible ethanol as
First eluent, the first macroporous absorbent resin is eluted by 2.0BV/h elution flow rate, collect the first eluent and first
Cross post liquid；Again by the use of the edible ethanol that 3.5BV volumetric concentrations are 40% as the second eluent, by 2.5BV/h elution flow rate pair
First macroporous absorbent resin is eluted, and then collects the second eluent, after the second eluent concentration and recovery ethanol, obtains solid
Thing content is 15% aqueous phase substance, and aqueous phase substance is spray-dried, and obtains the speed that ester catechin content is 65.33wt%
Molten tea powder；
Step 4：The first eluent in step 3 and first are crossed into post liquid and merge to obtain mixed liquor, the mixed liquor is added
To the second macroporous absorbent resin (the DM-2 resins of Tianjin sea light Chemical Co., Ltd. production) of energy selective absorption caffeine
(adsorption flow rate 2.0BV/h) is adsorbed, the second macroporous absorbent resin is eluted with water, second is collected and crosses post liquid and water
Washing lotion, then by the use of the edible ethanol that 3.5BV volumetric concentrations are 25% as the 3rd eluent, by 2.0BV/h elution flow rate pair
Second macroporous absorbent resin is eluted, and the 3rd eluent is collected, by the aqueous phase substance after the 3rd eluent concentration and recovery ethanol
It is spray-dried, obtains the instant tea powder that caffeine content is 24.72wt%；
Post liquid is crossed by second and water lotion mixes, and is then filtered, is then carried out obtained filtered fluid instead with reverse osmosis membrane
Infiltration is handled, and collects reverse osmosis concentrated liquid and counter-infiltration permeate, the reverse osmosis concentrated liquid is carried out to be spray-dried non-ester
Type catechin content is 42.43wt% instant tea powder；
Step 5：Counter-infiltration permeate in step 4 is added to the ion exchange resin for being capable of selective absorption theanine
(732 cationic ion-exchange resins of Shanghai Huazhen Science and Technology Co., Ltd.'s production) are adsorbed (adsorption flow rate 4.5BV/h), then are used
4.5BV molar concentrations be 0.1mol/L sodium hydroxide solution as the 4th eluent, by 3.0BV/h elution flow rate to ion
Exchanger resin is eluted, and then collects the 4th eluent, and the aqueous phase substance after the 4th eluent concentration and recovery ethanol is carried out
Dry, obtain the instant tea powder that theanine content is 16.90wt%.
Embodiment 3
A kind of tea Processing method, comprises the following steps：
Step 1：5 kilograms of large white teas (tea raw material) are weighed, are with deionized water or pure water dose volume concentration
65% edible ethanol, the volumetric concentration that 75 kilograms are added into 5 kilograms of large green tea is temperature of 65% edible ethanol at 50 DEG C
Under carry out extraction 30min, repeat extraction 2 times, tea grounds be collected by filtration, merge filtered fluid, obtain the first extract solution；
Step 2：The pectase (enzyme activity 10000u/g) of 5 grams of addition, 5 grams of cellulase (enzymes into step 1 gained tea grounds
Work is 100000u/g) and 75 kilograms of water 50min is extracted at a temperature of 50 DEG C, repeat extraction 2 times, filtering, merge filtered fluid
The second extract solution is obtained, then the second extract solution is filtered with the ceramic membrane that aperture is 0.2~0.5 μm, filtered fluid is used cut again
The milipore filter for staying molecular weight to be 30KD is filtered, and collects trapped fluid, trapped fluid is concentrated successively, is spray-dried, obtains tea
Polyoses content is 40.47wt% instant tea powder；
Step 3：First extract solution obtained by step 1 is subjected to the supernatant after centrifugation removal of impurities and carries out concentration and recovery ethanol, is obtained
Aqueous phase concentrate, aqueous phase concentrate is obtained into clarified solution using the ceramic membrane filter that aperture is 0.2~0.5 μm, by the clarified solution
It is added to the first macroporous absorbent resin (Cangzhou Bon Adsorption Material Science and Technology Co., Ltd's life of energy selective absorption ester catechin
The HPD100 resins of production) adsorbed (adsorption flow rate 2.0BV/h), then with the edible second that 4.0BV volumetric concentrations are 15%
Alcohol is eluted to the first macroporous absorbent resin by 2.5BV/h elution flow rate as the first eluent, collects the first eluent
Post liquid is crossed with first；Again by the use of the edible ethanol that 5.0BV volumetric concentrations are 50% as the second eluent, by 2.0BV/h elution
Flow velocity elutes to the first macroporous absorbent resin, then collects the second eluent, after the second eluent concentration and recovery ethanol,
The aqueous phase substance that solid content is 15% is obtained, aqueous phase substance is spray-dried, obtaining ester catechin content is
70.45wt% instant tea powder；
Step 4：The first eluent in step 3 and first are crossed into post liquid and merge to obtain mixed liquor, the mixed liquor is added
The second macroporous absorbent resin (the D4020 resins of Shandong Shandong limited company production) to energy selective absorption caffeine enters
Row absorption (adsorption flow rate 2.0BV/h), is eluted with water to the second macroporous absorbent resin, is collected second and is crossed post liquid and washing
Liquid, then by the use of the edible ethanol that 4.0BV volumetric concentrations are 15% as the 3rd eluent, by 2.0BV/h elution flow rate to the
Two macroporous absorbent resins are eluted, and collect the 3rd eluent, the aqueous phase substance after the 3rd eluent concentration and recovery ethanol is entered
Row spray drying, obtain the instant tea powder that caffeine content is 23.87wt%；
Post liquid is crossed by second and water lotion mixes, and is then filtered, is then carried out obtained filtered fluid instead with reverse osmosis membrane
Infiltration is handled, and collects reverse osmosis concentrated liquid and counter-infiltration permeate, the reverse osmosis concentrated liquid is carried out to be spray-dried non-ester
Type catechin content is 42.99wt% instant tea powder；
Step 5：Counter-infiltration permeate in step 4 is added to the ion exchange resin for being capable of selective absorption theanine
(the D301 weak anion exchange resins of Shanghai Huazhen Science and Technology Co., Ltd.'s production) is adsorbed (adsorption flow rate 2.5BV/h),
Again by the use of the sodium hydroxide solution that 3.0BV molar concentrations are 0.1mol/L as the 4th eluent, by 3.0BV/h elution flow rate pair
Ion exchange resin is eluted, and the 4th eluent is then collected, by the aqueous phase substance after the 4th eluent concentration and recovery ethanol
It is dried, obtains the instant tea powder that theanine content is 18.75wt%.
In summary, tea Processing method provided by the invention, the present invention prepare tea simultaneously by a technological process
Instant tea that instant tea that polyoses content is more than 20wt%, ester catechin content are more than 50wt%, caffeine content are
The instant tea and theanine content that more than 20wt% instant tea, non-ester catechin content are more than 40wt% be 15wt% with
On instant tea, effectively increase the utilization rate of tea raw material, while improve value-added content of product；The method that the present invention uses
In without using poisonous and harmful reagent, relate generally to a kind of organic solvent of edible ethanol, method is more green, safe operation,
The safety of product is also ensure that, before functional food, cakes and sweetmeats product, Medicines and Health Product and cosmetic field all have wide application
Scape.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair
The equivalent structure or equivalent flow conversion that bright description is made, or directly or indirectly it is used in other related technology necks
Domain, it is included within the scope of the present invention.
Claims (7)
1. a kind of tea Processing method, it is characterised in that comprise the following steps：
Step 1：Into tea raw material add 5~20 times volumetric concentration for 50~90% edible ethanols carry out extraction 20~
60min, repeat to extract, tea grounds is collected by filtration, merge filtered fluid and obtain the first extract solution；
Step 2：The enzyme and tea raw material 5~20 that weight is 0.5~5.0wt% of tea raw material are added into step 1 gained tea grounds
Water again extracts 20~60min at a temperature of 30~60 DEG C, repeats to extract, filtering, merges filtered fluid and obtains the second extract solution, then
Membrane filtration is carried out to the second extract solution and obtains trapped fluid, trapped fluid is concentrated successively, dried, it is 20wt% to obtain content of tea polysaccharide
Instant tea above, wherein, the enzyme is one or both of pectase and cellulase；
Step 3：First extract solution obtained by step 1 is subjected to the supernatant after centrifugation removal of impurities and carries out concentration and recovery ethanol, obtains aqueous phase
Concentrate, aqueous phase concentrate is obtained into clarified solution using the ceramic membrane filter that aperture is 0.2~0.5 μm, the clarified solution is added
The first macroporous absorbent resin to energy selective absorption ester catechin is adsorbed, and is then with 1.0~5.0BV volumetric concentrations
5~20% edible ethanol is entered as the first eluent by 1.0~3.5BV/h elution flow rate to the first macroporous absorbent resin
Row elution, collect the first eluent and first and cross post liquid；Again with the edible ethanol that 1.0~6.0BV volumetric concentrations are 30~75%
As the second eluent, the first macroporous absorbent resin is eluted by 1.0~3.5BV/h elution flow rate, then collects
Two eluents, the aqueous phase substance after the second eluent concentration and recovery ethanol is dried, obtaining ester catechin content is
More than 50wt% instant tea；
Step 4：The first eluent in step 3 and first are crossed into post liquid and merge to obtain mixed liquor, the mixed liquor is added to energy
Second macroporous absorbent resin of selective absorption caffeine is adsorbed, and the second macroporous absorbent resin is eluted with water, is received
Collection second crosses post liquid and water lotion, is then used as the 3rd elution by the use of the edible ethanol that 1.0~6.0BV volumetric concentrations are 10~50%
Liquid, the second macroporous absorbent resin is eluted by 1.0~3.5BV/h elution flow rate, collect the 3rd eluent, the 3rd is washed
Aqueous phase substance after de- liquid concentration and recovery ethanol is dried, and obtains the instant tea that caffeine content is more than 20wt%；
Post liquid is crossed by second and water lotion mixes, and then filters, obtained filtered fluid is then subjected to counter-infiltration with reverse osmosis membrane
Processing, collect reverse osmosis concentrated liquid and counter-infiltration permeate, by the reverse osmosis concentrated liquid it is dry non-ester catechin content
For more than 40wt% instant tea；
Step 5：Counter-infiltration permeate in step 4 is added to the ion exchange resin progress for being capable of selective absorption theanine
Absorption, then by the use of the sodium hydroxide solution that 0.5~5.0BV molar concentrations are 0.01~0.2mol/L as the 4th eluent, by 1.0
~3.5BV/h elution flow rate is eluted to ion exchange resin, then collects the 4th eluent, and the 4th eluent is concentrated
Aqueous phase substance after recovery ethanol is dried, and obtains the instant tea that theanine content is more than 15wt%.
2. tea Processing method according to claim 1, it is characterised in that the Extracting temperature in step 1 is 30~90 DEG C.
3. tea Processing method according to claim 1, it is characterised in that first extract solution is according to step 1 weight
Extract 2~3 times again, tea grounds is collected by filtration, merge filtered fluid, obtain the first extract solution；Second extract solution is according to step 2
Extraction 2~3 times is repeated, filtering, merges filtered fluid, obtains the second extract solution.
4. tea Processing method according to claim 1, it is characterised in that the concrete operations of the membrane filtration in step 2 are：
It is again first 10~70KD's with molecular cut off with the ceramic membrane filter that aperture is 0.2~0.5 μm, filtered fluid by the second extract solution
Milipore filter is filtered, and obtains trapped fluid.
5. tea Processing method according to claim 1, it is characterised in that the adsorption flow rate control adsorbed in step 3 exists
0.5~2.0BV/h；The adsorption flow rate adsorbed in step 4 is controlled in 0.5~3.0BV/h；Absorption flow control in step 5 is 1.0
~3.5BV/h speed.
6. tea Processing method according to claim 1, it is characterised in that the mode of the filtering in step 1 and step 2 is
Centrifugation, filter-cloth filtering, plate-frame filtering or vibratory sieve filtering in any one.
7. tea Processing method according to claim 1, it is characterised in that the tea raw material is green tea, white tea or crow
Imperial tea.
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| CN109251252A (en) * | 2018-07-25 | 2019-01-22 | 云南茶农生物产业有限责任公司 | A kind of technique that membrane separation technique combines cooling precipitation method to prepare tea polysaccharide, tea polyphenols, caffeine and theanine |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699586A (en) * | 2005-04-29 | 2005-11-23 | 西北大学 | Process for extracting tea polyphenol, caffeine as a byproduct thereof and tea polysaccharide from tea |
| CN101016344A (en) * | 2007-02-13 | 2007-08-15 | 上海师范大学 | Process of preparing high purity tea polysaccharide |
| CN101508690A (en) * | 2009-03-17 | 2009-08-19 | 林峰 | Novel methods for simultaneously extracting tea polyphenol, tea polysaccharide and caffeinum from tea |
| CN101986855A (en) * | 2009-07-30 | 2011-03-23 | 温尧林 | Method for preparing low-caffeine high-tea polyphenol tea powder |
| CN102675317A (en) * | 2012-05-29 | 2012-09-19 | 福建省安溪县南洋茶厂 | Method for extracting a plurality of kinds of active ingredients from tea leaves simultaneously |
| CN105712897A (en) * | 2015-01-25 | 2016-06-29 | 宜昌绿源生物技术有限公司 | Tea polyphenol extracting and recycling method |
| CN105968146A (en) * | 2016-05-11 | 2016-09-28 | 张铰铣 | Tea polyphenol production process for producing plurality of products by one time of feeding |
-
2017
- 2017-11-20 CN CN201711154830.XA patent/CN107712169B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699586A (en) * | 2005-04-29 | 2005-11-23 | 西北大学 | Process for extracting tea polyphenol, caffeine as a byproduct thereof and tea polysaccharide from tea |
| CN101016344A (en) * | 2007-02-13 | 2007-08-15 | 上海师范大学 | Process of preparing high purity tea polysaccharide |
| CN101508690A (en) * | 2009-03-17 | 2009-08-19 | 林峰 | Novel methods for simultaneously extracting tea polyphenol, tea polysaccharide and caffeinum from tea |
| CN101986855A (en) * | 2009-07-30 | 2011-03-23 | 温尧林 | Method for preparing low-caffeine high-tea polyphenol tea powder |
| CN102675317A (en) * | 2012-05-29 | 2012-09-19 | 福建省安溪县南洋茶厂 | Method for extracting a plurality of kinds of active ingredients from tea leaves simultaneously |
| CN105712897A (en) * | 2015-01-25 | 2016-06-29 | 宜昌绿源生物技术有限公司 | Tea polyphenol extracting and recycling method |
| CN105968146A (en) * | 2016-05-11 | 2016-09-28 | 张铰铣 | Tea polyphenol production process for producing plurality of products by one time of feeding |
Non-Patent Citations (1)
| Title |
|---|
| 曹博等: "茶多酚、茶多糖和茶氨酸的联合提取工艺研究", 《现代化工》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109251252A (en) * | 2018-07-25 | 2019-01-22 | 云南茶农生物产业有限责任公司 | A kind of technique that membrane separation technique combines cooling precipitation method to prepare tea polysaccharide, tea polyphenols, caffeine and theanine |
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