CN102675317A - Method for extracting a plurality of kinds of active ingredients from tea leaves simultaneously - Google Patents
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Abstract
The invention discloses a method for extracting a plurality of kinds of active ingredients from tea leaves simultaneously. Steps include subjecting tea leaves to light wave digestion and filteration; absorbing caffeine and theanine by cationic resins, obtaining caffeine and theanine through elution; absorbing tea polyphenols by adsorbent resins and obtaining tea polyphenols through elution; and collecting effluent liquids passing through cationic resins and adsorbent resins, and concentrating, deproteinizing and precipitating effluent liquids with ethanol to obtain tea polysaccharide. The method has the advantages that water serves as solvents, light wave extracting is used, the time is short, extraction rate is high, loss of active substances is little, and the cost is low; four kinds of active ingredients of caffeine, theanine, tea polyphenols and tea polysaccharide can be extracted simultaneously; adsorbent resins are used, the process is simple, energy consumption is low, pollution is small, and resins are easy to regenerate and can be recycled; and extraction rate is high, separating effect is good, and the purity of obtained active ingredients is high.
Description
Technical field
The invention belongs to field of deep tea processing technology, especially relate to a kind of method of from tealeaves, extracting plurality of active ingredients simultaneously.
Background technology
In China, it is historical of long standing and well established to drink tea, large contingent.China's tea resources is abundant, but utilization ratio is extremely low, except that the tealeaves that is processed to high-grade goods tea on a small quantity, and a large amount of be low and middle-grade tea (all existence in various degree unsalable).There are some researches show that the effective constituent of low and middle-grade tea is generally poor unlike high-grade tea, some composition also exceeds high-grade tea on the contrary.Therefore, greatly develop deep tea processing technology, therefrom extract in the low-grade tea and reclaim the multiple meaning that effective constituent has the unsalable situation of the low and middle-grade tea of change, economizes on resources and increase farmers' income.
Along with tealeaves research deepen continuously and with the intersection and the infiltration of medical research, it is found that materials such as being rich in theine, theanine, tea-polyphenol, TPS and VITAMINs in the tealeaves.Theine has cardiac stimulant, diuresis and effect such as anti-oxidant, is the important medical material and the additive of F&B; Medical functions such as that theanine has is antitumor, hypertensive tranquilizing, promotion nerve growth; Tea-polyphenol has different physiological roles; It is not only good natural antioxidants; Also have enhancing body resistibility, anti-cancer, antitumor, radioprotective, antibacterial, antiviral, hypoglycemic, reducing blood-fat, preventing cardiovascular disease, anti-ageing, prevent different physiological roles such as skin ultraviolet damage, have broad prospect of application in fields such as grease, food, medicine, daily-use chemical industry, light industries; TPS has multiple physiological action; Like radioprotective, anticoagulation and antithrombotic, hypoglycemic etc.; It can also the enhancing body immunologic function; Effects such as that experiment proof TPS has is hypotensive, reducing heart rate, anoxia, increase coronary flow can also reducing blood-fat, arteriosclerosis, anti-inflammatory and anticancer etc.Therefore, from tealeaves, extract safely and effectively and separate compositions such as theine, theanine, tea-polyphenol and TPS, have important and practical meanings.
Domestic process patent about functional ingredient such as extraction tea-polyphenol from tealeaves has a lot; Such as one Chinese patent application: 93105511.3, ZL98111572.1,200510042629.3 and Chinese patent ZL200810107066.5, ZL200910111284.0 etc. just process for extracting is reported; But they all exist the active component content that extracts low, extract rate variance or have drawbacks such as dissolvent residual, and also can't from tealeaves, extract various active compositions such as theine, theanine, tea-polyphenol and TPS simultaneously.At present; The relevant patent of from tealeaves, extracting plurality of active ingredients simultaneously is also fewer; Wherein one Chinese patent application 200410015905.2 discloses a kind of microwave extract method that from tealeaves, comprehensively extracts TPS, tea-polyphenol, theanine and trimethyl-xanthine, but resulting tea-polyphenol purity of this method and yield are all undesirable; In addition, one Chinese patent application 201010141764.4 also discloses the solvent-extraction process of TPS, theanine, tea-polyphenol and theine in a kind of comprehensive separation and Extraction tealeaves that adopts inorganic zeolite molecular sieve, but whole extraction yield still is relatively poor.Other also has some process for extracting, do not enumerate one by one at this, but they also all exists shortcoming separately.Therefore, the effective constituent of how fully extracting efficiently in the tealeaves is still problem demanding prompt solution.
Light-wave cooker is to utilize the light wave producer to produce luminous energy and heat energy rapidly, and food is heated from outside to inside, and light wave has that rate of heating is fast, homogeneous heating, can keep the nutritive ingredient of food plurality of advantages such as not lose to greatest extent.The appearance of light wave has thoroughly solved uneven, the living ripe inhomogenous phenomenon of microwave heating, and thermo-efficiency is higher, and can reduce the forfeiture of moisture as far as possible.
Summary of the invention
To the problems referred to above, the present invention is based on the light wave intensifying technology, a kind of method of from tealeaves, extracting plurality of active ingredients such as theine, theanine, tea-polyphenol, TPS etc. simultaneously is provided.
For achieving the above object, technical scheme proposed by the invention is: a kind of method of from tealeaves, extracting plurality of active ingredients simultaneously is characterized in that: comprise the steps:
Step 1: with tealeaves and water by weight being 1:10-15, under the light wave effect, 40-60 ℃ soaking and extracting 10-30 minute, cross and filter tea grounds and soaked extract, and concentrated soaked extract;
Step 2: with resin cation(R.C.) with distilled water immersion after, after bronsted lowry acids and bases bronsted lowry re-treatment 2 times, with acid transition, be washed till neutrality with zero(ppm) water at last again;
Step 3: with polymeric adsorbent with distilled water immersion after, with washing with alcohol to colourless;
Step 4: the infusion of tea extracting solution in the step 1 is passed through cationic resin column with the speed of 25-40mL/min, and effluent passes through adsorption resin column with the speed of 20-30mL/min again.Earlier remove the theine on the cationic resin column with chloroform, concentrate and reclaim chloroform, drying obtains theine; Use the pH value to be the theanine on the citrate buffer solution wash-out cationic resin column of 6.5-7.5 again, concentrate, low temperature crystallization gets theanine;
Step 5: adsorption column uses the ethanol elution of volume ratio as 60-80%, concentrates to reclaim ethanol, the dry tea-polyphenol that gets;
Step 6: will be effluent through cationic resin column and adsorption resin column concentrate, remove the Partial Protein in the TPS with the Sevag method after, add ethanol TPS precipitated, the dry TPS that gets.
Preferably, said tealeaves is the tankage in low-grade tea and the Tea Production, and described tealeaves is through being crushed between 60 orders-80 order.
Further, water of the present invention is deionized water.
Further, step 1 also comprises tea grounds added water again, tea grounds and water by weight 1:5-10 once more under the light wave effect in 40-60 ℃ of soaking and extracting 10-30 minute, remove by filter filter residue, merge 2 times soaked extract.
Preferably, the described soaked extract of step 1 is concentrated to 1/5 of original volume.
Further, acid is 5% hydrochloric acid in the step 2, and alkali is 5% sodium hydroxide.
Further, in the step 2, described resin cation(R.C.) is preferably a kind of among WA-2, the D135,003 * 7,001 * 4 or 001 * 7.
Further, the polymeric adsorbent described in the step 3 is preferably a kind of among 92-2,92-3, NK-S3, XAD-7, D4020 or the HP-21.
Further, described theanine liquid concentrator carries out the low temperature crystallization method and can separate out theanine for placing to spend the night under-10 ℃.
Further, describedly be filtered into filter press, filter bag filtration, membrane sepn filtration etc.; Described simmer down to distillation concentrates, vacuum concentration; Described drying is lyophilize, vacuum-drying or spraying drying.
Adopt technique scheme, beneficial effect of the present invention is:
1) with water is solvent, adopts light wave to extract the effective constituent in the tealeaves, have short, advantage such as efficient is high, the active substance loss is few of extraction time;
2) disposable extraction theine, theanine, tea-polyphenol and four kinds of effective constituents of TPS;
3) adopt resin adsorption method to separate the effective constituent in the tealeaves, technology is simple, less energy consumption, pollution are little, and resin regeneration is easy, can recycle;
4) extraction yield is high, and good separating effect, and each active constituent content is high, theine purity >=99%, extraction yield >=1.8%; Theanine purity >=82%, extraction yield >=0.8%; Tea-polyphenol purity >=99%, extraction yield >=15.1%; The total sugar content of TPS >=70%, extraction yield are 1.6%.
Description of drawings
Fig. 1 is the basic flow sheet of tealeaves process for extracting of the present invention.
Embodiment
Below in conjunction with accompanying drawing and specific embodiment, the present invention is further specified.
As shown in Figure 1, the idiographic flow of the method for from tealeaves, extracting plurality of active ingredients simultaneously of the present invention is following:
1) with tealeaves and water by weight being 1:10-15, under the light wave effect, 40-60 ℃ soaking and extracting 10-30 minute; Filter, tea grounds adds water again, tea grounds and water by weight 1:5-10 once more under the light wave effect in 40-60 ℃ of soaking and extracting 10-30 minute; Remove by filter filter residue; Merge 2 times soaked extract, and concentrated soaked extract, concentrated volume preferably is concentrated to 1/5 of original volume;
2) with resin cation(R.C.) with distilled water immersion after, after bronsted lowry acids and bases bronsted lowry re-treatment 2 times, with acid transition, be washed till neutrality with zero(ppm) water at last, preferred 5% hydrochloric acid and 5% sodium hydroxide again;
3) with polymeric adsorbent with distilled water immersion after, with washing with alcohol to colourless;
4) above-mentioned infusion of tea extracting solution is passed through cationic resin column with the speed of 25-40mL/min, effluent passes through adsorption resin column with the speed of 20-30mL/min again.Earlier remove the theine on the cationic resin column with chloroform, concentrate and reclaim chloroform, drying obtains theine; Use the pH value to be the theanine on the citrate buffer solution wash-out cationic resin column of 6.5-7.5 again, concentrate, low temperature crystallization gets theanine;
5) adsorption column concentrates and reclaims ethanol with 60-80% ethanol (volume ratio) wash-out, the dry tea-polyphenol that gets;
6) will be effluent through cationic resin column and adsorption resin column concentrate, remove the Partial Protein in the TPS with the Sevag method after, add ethanol TPS precipitated, the dry TPS that gets.
In the present invention, described tealeaves is preferably the tankage in low-grade tea and the Tea Production, and tealeaves is through being crushed between 60 orders-80 order.
In the present invention, described water is preferably deionized water.
In the present invention, described resin cation(R.C.) is preferably a kind of among WA-2, the D135,003 * 7,001 * 4 or 001 * 7.
In the present invention, described polymeric adsorbent is preferably a kind of among 92-2,92-3, NK-S3, XAD-7, D4020 or the HP-21.
In the present invention, the theanine liquid concentrator is carried out low temperature crystallization, generally placing spends the night under-10 ℃ can separate out theanine.
In the present invention, described filtration can be membrane sepn filtration, filter bag separation, filter press etc.,, to concentrate can be distillation, vacuum concentration, drying can be lyophilize, vacuum-drying and spraying drying.
Embodiment 1
1) tea dust 500g adds water 5Kg, under the light wave effect; 40-60 ℃ soaking and extracting 10-30 minute; Filter, tea grounds add water 5Kg again and under the light wave effect in 40-60 ℃ of soaking and extracting 10-30 minute, remove by filter filter residue; Merge 2 times soaked extract, and concentrated soaked extract is to 1/5 of original volume;
2) with resin cation(R.C.) with distilled water immersion after, after bronsted lowry acids and bases bronsted lowry re-treatment 2 times, with acid transition, be washed till neutrality with zero(ppm) water at last again, described resin cation(R.C.) is WA-2;
3) with polymeric adsorbent with distilled water immersion after, to colourless, described polymeric adsorbent is 92-2 with washing with alcohol;
4) above-mentioned infusion of tea extracting solution is passed through cationic resin column with the speed of 25mL/min, effluent passes through adsorption resin column with the speed of 20mL/min again.Earlier remove the theine on the cation seperation column with chloroform, concentrate and reclaim chloroform, drying obtains the 9.5g theine, and extraction yield is 1.9%, and purity is 99.2%.Using the pH value again is the theanine on 7.2 the citrate buffer solution wash-out cationic resin column, and elutriant concentrates, and is cooled to-10 ℃, obtains the 4.2g theanine after the crystallization, and extraction yield is 0.84%, and purity is 82.7%;
5) adsorption column concentrates and reclaims ethanol with 80% ethanol (volume ratio) wash-out, and drying obtains the 77.2g tea-polyphenol, and extraction yield is 15.44%, and purity is 99%;
6) will be effluent through cation seperation column and adsorption column concentrate, remove the Partial Protein in the TPS with the Sevag method after, add ethanol TPS precipitated, drying obtains the 8.35g TPS, extraction yield is 1.67%, total sugar content is 72%.
Embodiment 2
1) tea dust 500g adds water 6Kg, under the light wave effect; 40-60 ℃ soaking and extracting 10-30 minute; Filter, tea grounds add water 4Kg again and under the light wave effect in 40-60 ℃ of soaking and extracting 10-30 minute, remove by filter filter residue; Merge 2 times soaked extract, and concentrated soaked extract is to 1/5 of original volume;
2) with resin cation(R.C.) with distilled water immersion after, after hydrochloric acid and sodium hydroxide re-treatment 2 times, with hydrochloric acid transition, be washed till neutrality with zero(ppm) water at last again, described resin cation(R.C.) is D135;
3) with polymeric adsorbent with distilled water immersion after, to colourless, described polymeric adsorbent is 92-3 with washing with alcohol;
4) above-mentioned infusion of tea extracting solution is passed through cationic resin column with the speed of 35mL/min, effluent passes through adsorption resin column with the speed of 25mL/min again.Earlier remove the theine on the cation seperation column with chloroform, concentrate and reclaim chloroform, drying obtains the 9.0g theine, and extraction yield is 1.8%, and purity is 99.3%.Using the pH value again is the theanine on 6.5 the citrate buffer solution wash-out cationic resin column, and elutriant concentrates, and is cooled to-10 ℃, obtains the 4g theanine after the crystallization, and extraction yield is 0.8%, and purity is 83.1%;
5) adsorption column concentrates and reclaims ethanol with 60% ethanol (volume ratio) wash-out, and drying obtains the 75.5g tea-polyphenol, and extraction yield is 15.1%, and purity is 99.2%;
6) will be effluent through cation seperation column and adsorption column concentrate, remove the Partial Protein in the TPS with the Sevag method after, add ethanol TPS precipitated, drying obtains the 8.25g TPS, extraction yield is 1.65%, total sugar content is 71.6%.
Embodiment 3
1) tea dust 500g adds water 7.5Kg, under the light wave effect; 40-60 ℃ soaking and extracting 10-30 minute; Filter, tea grounds add water 2.5Kg again and under the light wave effect in 40-60 ℃ of soaking and extracting 10-30 minute, remove by filter filter residue; Merge 2 times soaked extract, and concentrated soaked extract is to 1/5 of original volume;
2) with resin cation(R.C.) with distilled water immersion after, after 5% hydrochloric acid and 5% sodium hydroxide re-treatment 2 times, with 5% hydrochloric acid transition, be washed till neutrality with zero(ppm) water at last again, described resin cation(R.C.) is preferably 003 * 7;
3) with polymeric adsorbent with distilled water immersion after, to colourless, described polymeric adsorbent is NK-S3 with washing with alcohol;
4) above-mentioned infusion of tea extracting solution is passed through cationic resin column with the speed of 40mL/min, effluent passes through adsorption resin column with the speed of 30mL/min again.Earlier remove the theine on the cation seperation column with chloroform, concentrate and reclaim chloroform, drying obtains the 9.77g theine, and extraction yield is 1.95%, and purity is 99%.Using the pH value again is the theanine on 6.5 the citrate buffer solution wash-out cationic resin column, and elutriant concentrates, and is cooled to-10 ℃, obtains the 4.3g theanine after the crystallization, and extraction yield is 0.86%, and purity is 82.4%;
5) adsorption column concentrates and reclaims ethanol with 70% ethanol (volume ratio) wash-out, and drying obtains the 76.3g tea-polyphenol, and extraction yield is 15.26%, and purity is 99.1%;
6) will be effluent through cation seperation column and adsorption column concentrate, remove the Partial Protein in the TPS with the Sevag method after, add ethanol TPS precipitated, drying obtains the 8.0g TPS, extraction yield is 1.6%, total sugar content is 72.8%.
Embodiment 4
1) tea dust 500g adds water 7Kg, under the light wave effect; 40-60 ℃ soaking and extracting 10-30 minute; Filter, tea grounds add water 3Kg again and under the light wave effect in 40-60 ℃ of soaking and extracting 10-30 minute, remove by filter filter residue; Merge 2 times soaked extract, and concentrated soaked extract is to 1/5 of original volume;
2) with resin cation(R.C.) with distilled water immersion after, after 5% hydrochloric acid and 5% sodium hydroxide re-treatment 2 times, with 5% hydrochloric acid transition, be washed till neutrality with zero(ppm) water at last again, described resin cation(R.C.) is 001 * 4;
3) with polymeric adsorbent with distilled water immersion after, to colourless, described polymeric adsorbent is preferably XAD-7 with washing with alcohol;
4) above-mentioned infusion of tea extracting solution is passed through cationic resin column with the speed of 30mL/min, effluent passes through adsorption resin column with the speed of 25mL/min again.Earlier remove the theine on the cation seperation column with chloroform, concentrate and reclaim chloroform, drying obtains the 9.8g theine, and extraction yield is 1.96%, and purity is 99.3%.Using the pH value again is the theanine on 6.8 the citrate buffer solution wash-out cationic resin column, and elutriant concentrates, and is cooled to-10 ℃, obtains the 4.55g theanine after the crystallization, and extraction yield is 0.91%, and purity is 82%;
5) adsorption column concentrates and reclaims ethanol with 75% ethanol (volume ratio) wash-out, and drying obtains the 76.5g tea-polyphenol, and extraction yield is 15.3%, and purity is 99.4%;
6) will be effluent through cation seperation column and adsorption column concentrate, remove the Partial Protein in the TPS with the Sevag method after, add ethanol TPS precipitated, drying obtains the 8.55g TPS, extraction yield is 1.71%, total sugar content is 70%.
Embodiment 5
1) tea dust 500g adds water 6Kg, under the light wave effect; 40-60 ℃ soaking and extracting 10-30 minute; Filter, tea grounds add water 5Kg again and under the light wave effect in 40-60 ℃ of soaking and extracting 10-30 minute, remove by filter filter residue; Merge 2 times soaked extract, and concentrated soaked extract is to 1/5 of original volume;
2) with resin cation(R.C.) with distilled water immersion after, after 5% hydrochloric acid and 5% sodium hydroxide re-treatment 2 times, with 5% hydrochloric acid transition, be washed till neutrality with zero(ppm) water at last again, described resin cation(R.C.) is 001 * 7;
3) with polymeric adsorbent with distilled water immersion after, to colourless, described polymeric adsorbent is D4020 with washing with alcohol;
4) above-mentioned infusion of tea extracting solution is passed through cationic resin column with the speed of 30mL/min, effluent passes through adsorption resin column with the speed of 20mL/min again.Earlier remove the theine on the cation seperation column with chloroform, concentrate and reclaim chloroform, drying obtains the 10.1g theine, and extraction yield is 2.02%, and purity is 99.1%.Using the pH value again is the theanine on 7.0 the citrate buffer solution wash-out cationic resin column, and elutriant concentrates, and is cooled to-10 ℃, obtains the 4.17g theanine after the crystallization, and extraction yield is 0.83%, and purity is 83.7%;
5) adsorption column concentrates and reclaims ethanol with 65% ethanol (volume ratio) wash-out, and drying obtains the 77.3g tea-polyphenol, and extraction yield is 15.5%, and purity is 99.1%;
6) will be effluent through cation seperation column and adsorption column concentrate, remove the Partial Protein in the TPS with the Sevag method after, add ethanol TPS precipitated, drying obtains the 8.14g TPS, extraction yield is 1.63%, total sugar content is 70.7%.
Although specifically show and introduced the present invention in conjunction with preferred embodiment; But the those skilled in the art should be understood that; In the spirit and scope of the present invention that do not break away from appended claims and limited; In form with on the details the present invention is made various variations, be protection scope of the present invention.
Claims (9)
1. a method of from tealeaves, extracting plurality of active ingredients simultaneously is characterized in that: comprise the steps:
Step 1: with tealeaves and water by weight being 1:10-15, under the light wave effect, 40-60 ℃ soaking and extracting 10-30 minute, cross and filter tea grounds and soaked extract, and concentrated soaked extract;
Step 2: with resin cation(R.C.) with distilled water immersion after, after bronsted lowry acids and bases bronsted lowry re-treatment 2 times, with acid transition, be washed till neutrality with zero(ppm) water at last again;
Step 3: with polymeric adsorbent with distilled water immersion after, with washing with alcohol to colourless;
Step 4: the infusion of tea extracting solution in the step 1 is passed through cationic resin column with the speed of 25-40mL/min, and effluent passes through adsorption resin column with the speed of 20-30mL/min again;
Earlier remove the theine on the cationic resin column with chloroform, concentrate and reclaim chloroform, drying obtains theine; Use the pH value to be the theanine on the citrate buffer solution wash-out cationic resin column of 6.5-7.5 again, concentrate, low temperature crystallization gets theanine;
Step 5: adsorption column uses the ethanol elution of volume ratio as 60-80%, concentrates to reclaim ethanol, the dry tea-polyphenol that gets;
Step 6: will be effluent through cationic resin column and adsorption resin column concentrate, remove the Partial Protein in the TPS with the Sevag method after, add ethanol TPS precipitated, the dry TPS that gets.
2. a kind of method of from tealeaves, extracting plurality of active ingredients simultaneously according to claim 1 is characterized in that: said tealeaves is the tankage in low-grade tea and the Tea Production, and described tealeaves is through being crushed between 60 orders-80 order.
3. a kind of method of from tealeaves, extracting plurality of active ingredients simultaneously according to claim 1 is characterized in that: described water is deionized water.
4. a kind of method of from tealeaves, extracting plurality of active ingredients simultaneously according to claim 1; It is characterized in that: step 1 also comprises tea grounds is added water again; Tea grounds and water by weight 1:5-10 once more under the light wave effect in 40-60 ℃ of soaking and extracting 10-30 minute; Remove by filter filter residue, merge 2 times soaked extract.
5. according to the described a kind of method of from tealeaves, extracting plurality of active ingredients simultaneously of the arbitrary claim of claim 1-4, it is characterized in that: the concentrated soaked extract described in the step 1 is to 1/5 of original volume.
6. according to the described a kind of method of from tealeaves, extracting plurality of active ingredients simultaneously of the arbitrary claim of claim 1-4, it is characterized in that: acid is 5% hydrochloric acid in the step 2, and alkali is 5% sodium hydroxide.
7. according to the described a kind of method of from tealeaves, extracting plurality of active ingredients simultaneously of the arbitrary claim of claim 1-4, it is characterized in that: in the step 2, described resin cation(R.C.) is a kind of among WA-2, the D135,003 * 7,001 * 4 or 001 * 7.
8. according to the described a kind of method of from tealeaves, extracting plurality of active ingredients simultaneously of the arbitrary claim of claim 1-4, it is characterized in that: the polymeric adsorbent described in the step 3 is a kind of among 92-2,92-3, NK-S3, XAD-7, D4020 or the HP-21.
9. according to the described a kind of method of from tealeaves, extracting plurality of active ingredients simultaneously of the arbitrary claim of claim 1-4, it is characterized in that: describedly be filtered into that filter press, filter bag filter, membrane sepn filters; Described simmer down to distillation concentrates, vacuum concentration; Described drying is lyophilize, vacuum-drying or spraying drying.
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| CN104130260A (en) * | 2014-07-07 | 2014-11-05 | 李华 | Caffeine extraction method |
| CN105866342A (en) * | 2016-05-13 | 2016-08-17 | 中国农业科学院茶叶研究所 | Method for quickly detecting biochemical components of fresh tea leaf sample |
| CN106543023A (en) * | 2016-11-04 | 2017-03-29 | 湖北宇隆茶业有限责任公司 | The processing method of the extracting method and tealeaf residue of tea polyphenols and theanine in tea old leaf |
| CN106937689A (en) * | 2017-04-21 | 2017-07-11 | 云南集彩民族文化创意有限公司 | A kind of super fine tea powder preparation method |
| CN106967128A (en) * | 2017-03-24 | 2017-07-21 | 合肥云都棉花有限公司 | A kind of extracting method of raffinose |
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| CN109251252A (en) * | 2018-07-25 | 2019-01-22 | 云南茶农生物产业有限责任公司 | A kind of technique that membrane separation technique combines cooling precipitation method to prepare tea polysaccharide, tea polyphenols, caffeine and theanine |
| CN110129169A (en) * | 2019-05-22 | 2019-08-16 | 遵义医科大学 | The method for reducing content of caffeine in distillation type tea wine |
| CN112806456A (en) * | 2020-12-29 | 2021-05-18 | 满山歌茶业(西双版纳)有限公司 | Preparation method of directly drinking theaflavin with natural tea fragrance |
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| CN107235854B (en) * | 2017-07-28 | 2019-11-15 | 贵州芳瑞堂生物科技有限公司 | A kind of extracting method of Theanine in Tea |
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| CN109251252A (en) * | 2018-07-25 | 2019-01-22 | 云南茶农生物产业有限责任公司 | A kind of technique that membrane separation technique combines cooling precipitation method to prepare tea polysaccharide, tea polyphenols, caffeine and theanine |
| CN110129169A (en) * | 2019-05-22 | 2019-08-16 | 遵义医科大学 | The method for reducing content of caffeine in distillation type tea wine |
| CN112806456A (en) * | 2020-12-29 | 2021-05-18 | 满山歌茶业(西双版纳)有限公司 | Preparation method of directly drinking theaflavin with natural tea fragrance |
| CN116019223A (en) * | 2022-12-07 | 2023-04-28 | 山东奥博森生物药业股份有限公司 | Probiotic composition |
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