CN100368528C - Bacterium for degrading pyrethroid pesticide residue and produced bacterium formulation - Google Patents
Bacterium for degrading pyrethroid pesticide residue and produced bacterium formulation Download PDFInfo
- Publication number
- CN100368528C CN100368528C CNB2005100225487A CN200510022548A CN100368528C CN 100368528 C CN100368528 C CN 100368528C CN B2005100225487 A CNB2005100225487 A CN B2005100225487A CN 200510022548 A CN200510022548 A CN 200510022548A CN 100368528 C CN100368528 C CN 100368528C
- Authority
- CN
- China
- Prior art keywords
- test
- bacterium
- jar
- culture
- produce
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides a degradation bacterium for eliminating pyrethroid pesticide residues, and a bactericidal agent produced by the degradation bacterium, which belongs to the high-technical field of biology. A bacterial strain which needs to be used is LFJS3-9, which belongs to pseudomonas sp. The present invention has a main biological characteristic of G<->, and the size of a thallus which forms a rod shape is 1.96 mu m * 0.62 mu m, wherein single flagella are on poles of the thallu; a nitric reduction test, a phenylalanine deaminase test, etc. are positive; a citric acid test, a gelatin liquefaction test, etc. are negative; the present invention can utilize amylaceum and diabetin and can not hydrolyze starch, and the bacterial strain can generate alkali during the process of growth. Products of the degradation bacterium through direct application enable the amount of the pesticide residues in crops to reduce by over 80 percent, so the problem that the pesticide residues are overproof in agricultural production is solved, and non-poisonous nuisance-free green farm products can be produced.
Description
One, technical field
The microbial inoculum of a kind of degrading pyrethroid pesticide residue bacterium of the present invention and production thereof belongs to biological high-tech field, is to utilize method of microorganism degraded chemical pesticide residual, is applicable to the production and the processing of green non-polluted farm product in the modern agriculture production.
Two, technical background
The use of chemical pesticide has promoted modern agricultural development widely, for the loss that reasons such as a large amount of due to illness insect pests cause has been retrieved in agriculture production, and greatly improved the efficiency and the mechanization degree of agriculture production, in the modernization of agriculture production, played immeasurable effect.But agricultural chemicals all is some toxic and harmful substances simultaneously, after they enter ecotope, has not only killed the deleterious biology to the mankind, also the ecosystem has been caused serious harm simultaneously.A large amount of uses of agricultural chemicals cause that pesticide residue exceed standard in the agricultural-food, have had a strong impact on the foreign exchange earning of people health and agricultural-food.Ministry of Agriculture's investigation statistics shows, this class agricultural chemicals is one of three main in the vegetables, fruit of the outlet of present China pesticide residues, European Union rose in the pesticide residue new standard of enforcement on July 1st, 2000, the change of pyrethroid insecticides maximum residue limit standard is maximum, standard has improved tens to hundreds of times, cause this pesticide residue rank front two in Chinese exports tealeaves that exceeds standard, the tea export of China is brought seriously influence.Along with the raising of people's living standard and the enhancing of environmental protection consciousness, the whole society also more and more pays close attention to direct harm and potential impact that pesticide residue are brought, and people press for the green agricultural product of the cleaning of non agricultural chemical residuum pollution.But agricultural form and demographic situation from present China, use to stop agricultural chemicals that to reduce farm output be impracticable as the nuisanceless production model of cost, agricultural chemicals also will continue to bring into play enormous function as the effective means of control disease and pest in agriculture production.How solving this double-barreled question in the agriculture production just becomes the research focus of each subject.Laboratory and real application research all show pesticide residue " storehouse " capacity that utilizes microorganism to degrade to eliminate in the soil, thereby reduce the pesticide residue in the agricultural-food such as grain, vegetables, tealeaves, and the quality and the economic worth that improve agricultural-food are feasible.
Three, summary of the invention
Technical problem the objective of the invention is at practical problems in the production practice and demand, develop out a kind of novel bacterium for degradating residual agricultural chemical, use this microbial inoculum can make the residual quantity of pyrethroid pesticides such as bifenthrin, Deltamethrin, Cypermethrin, fenvalerate, Fenvalerate, effective cypermethrin reduce more than 80%.
Technical scheme
The invention provides a kind of pyrethroid pesticide remained degradation bacteria of eliminating, its bacterial strain is that a strain gramstaining reaction negative bacterium LFJS3-9 (was deposited at Chinese microorganism strain and preserves management committee common micro-organisms center on October 12nd, 2005, culture presevation number is CGMCC NO.1477), belong to Rhodopseudomonas (Pseudomonas sp.) through identifying, thalline is shaft-like, about 1.96 * 0.62 μ m of size extremely give birth to single flagellum; Nitrate reduction test, phenylalanine deaminase test, oxidase test, catalase test, 3-ketone group lactose are measured, lipase (Tween80) is tested, denitrification is tested, the two hydrolysis experiment positives of arginine; It is negative that citrate test, gelatin liquification test, methyl red test, V.P. test, pyocyanin produce test, fluorochrome produces test; Can utilize glucose and fructose, can not hydrolyzed starch, produce alkali in the strain growth process, the Genbank number of landing of this bacterial strain 16S rDNA is DQ164217.
The technology of using above-mentioned degrading pyrethroid pesticide residue bacterium to produce microbial inoculum is: inclined-plane kind-shake bottle a kinds-seeding tank-production jar-product (pack formulation and be liquid bacterial agent or solid absorption microbial inoculum).
The degradation bacterium preparation that above-mentioned elimination is pyrethroid pesticide remained, its production method is:
1) pesticide residual degrading bacteria LFJS3-9 test tube kind is inoculated in broth culture and shakes in the bottle, shaking culture is to logarithmic phase;
2) above-mentioned cultured bacterial classification is inoculated into 500 liters of seeding tanks by 10% inoculum size of seed tank culture base, be cultured to logarithmic phase, the used culture medium prescription of seeding tank is: glucose 0.8%, (NH
4)
2SO
41%, K
2HPO
40.2%, MgSO
40.05%, NaCl 0.01%, CaCO
30.3%, yeast extract paste 0.02%, pH value 7.2-7.5;
3) seed liquor is inserted to produce by 10% the inoculum size of producing jar substratum jar cultivate, it is identical with the seed tank culture base to produce jar used substratum;
The air flow of sterile air is 1 in the culturing process of seeding tank and production jar: 0.6-1.2, stirring velocity is 180-240 rev/min, culture temperature is 30-35 ℃, the whole process incubation time is 48-60 hour, after the fermentation ends thalline quantity reach 1,000,000,000/more than the ml, fermentation is finished the back nutrient solution and is gone out jar and directly be distributed into liquid dosage form with plastic barrel or packing bottle or adopt peat to adsorb and be distributed into the solid fungicide formulation with packing bag.。
It is low, easy to use that the bacterium for degradating residual agricultural chemical that beneficial effect uses this invention to produce has the production use cost, and the advantage that removal effect is good is adapted at national grain oil ﹠ vegetable production export base or has the local big area of green food brand mark to promote the use of.
The present invention protects people health for preserving the ecological environment, and near the value that improves agricultural-food has great importance.Degradation bacteria LFJS3-9 has wider degraded spectrum, can make the residual quantity of pyrethroid pesticides such as bifenthrin, Deltamethrin, Cypermethrin, fenvalerate, Fenvalerate, effective cypermethrin reduce more than 80%, greatly reduce the workload in production and the use, reduced production and use cost.
Use this degradation bacterial agent in the normal growth process of farm crop, normally to use chemical pesticide to carry out pest control and guarantee that the agricultural chemicals residual content meets the green food requirement in the agricultural-food.And this bacterium is a Gram-negative bacteria, the effect that agricultural chemicals uses after the survival time in field suitably can not influence.
Use the degradation bacteria plot experiment in the tea place and show, use this degradation bacteria that the pesticide residue of pyrethroid are reduced more than 80%, make Tea Production reach the safety in production standard.The present invention has successfully solved the problem that exceeds standard of some pesticide residue in the agriculture production, has both given full play to the efficiently fast effect of chemical pesticide in plant pest management, can produce non-toxic and non-pollution green agricultural product again.
Four, embodiment
Below narrate embodiments of the invention.
The original seed of degrading pyrethroid pesticide residue bacterium LFJS3-9 of the present invention deposited on the culture dish activate, and measure degradation property, be inoculated on the test tube slant standby.The test tube kind of degrading pyrethroid pesticide residue bacterium LFJS3-9 is inoculated in contains 200ml broth culture 1000ml and shake in the bottle, constant-temperature shaking culture is prepared the inoculation seeding tank to logarithmic phase.500 liters of seeding tanks, 400 liters of charging capacitys, culture medium prescription is: glucose 0.8%, (NH
4)
2SO
41%, K
2HPO
40.2%, MgSO
40.05%, NaCl 0.01%, CaCO
30.3%, yeast extract paste 0.02%, pH value 7.2-7.5.The back 121 ℃ of high pressure moist heat sterilizations that finish that feed intake, be cooled to 33 ℃ after, the above-mentioned cultured bottle bacterial classification that shakes is inoculated into 500 liters of seeding tanks by 10% inoculum size, be cultured to logarithmic phase, stirring velocity is 220 rev/mins, sterile air feeding amount is 1: 0.8.The seed liquor that arrives logarithmic phase is produced jar by 10% inoculum size access cultivate, it is identical with the seed tank culture base to produce the used medium component of jar.Produce 5 tons of tankages, 4.5 tons of charging capacitys.Production jar 1.1kg/cm after feeding intake
2Pressure under, 121 ℃ of high pressure moist heat sterilizations, below the sterilization postcooling to 35 ℃, logical sterile air keeps sterile state standby.A postvaccinal production jar temperature is controlled at 30-35 ℃, and the air flow of sterile air is 1 in the culturing process of production jar: 0.8-1.0, and stirring velocity is 240 rev/mins, the whole process flow incubation time is 48-60 hour.After the fermentation ends thalline quantity reach 1,000,000,000/more than the ml.
Fermentation is finished the back nutrient solution and is gone out jar and directly be distributed into liquid dosage form with plastic barrel or packing bottle or adopt peat to adsorb and be distributed into the solid fungicide formulation with packing bag.
Product with the aforesaid method gained carries out field pesticides residue degrading experiment and demonstration.
(component is: NH at minimal medium
4NO
31.00g, MgSO
47H
2O 0.10g, (NH
4)
2SO
40.5g, KH
2PO
40.50g, NaCl 0.50g, K
2HPO
41.50g, water 1000mL, pH7.0.) in add the following agricultural chemicals that final concentration is 20mg/L respectively: Cypermethrin, bifenthrin, fenvalerate, effective cypermethrin, Fenvalerate, Deltamethrin, inoculation degradation bacteria LFJS3-9, and establish the substratum that does not connect bacterium and be contrast.After the shaking culture 3 days, extract agricultural chemicals and use gas Chromatographic Determination pesticide residue (measuring) by Ministry of Agriculture food quality supervision verification test center (Jinan).From the result, find out, more than various agricultural chemicals residual quantity after degradation bacteria is handled reduced by 94.72%, 83.44%, 90.89%, 87.58%, 97.01%, 84.65% (table 1) respectively.
Table 1 degradation bacteria LFJS3-9 is to the degradation effect (deriving from Ministry of Agriculture food quality supervision verification test center (Jinan) survey report) of Cypermethrin, bifenthrin, fenvalerate, effective cypermethrin, Fenvalerate, Deltamethrin
| Cypermethrin (mgL -1) | Bifenthrin (mgL -1) | Fenvalerate (mgL -1) | Effective cypermethrin (mgL -1) | Fenvalerate (mgL -1) | Deltamethrin (mgL -1) | |
| Contrast | 10.6 | 9.18 | 5.60 | 22.7 | 9.02 | 41.5 |
| Bacterium liquid | 0.56 | 1.52 | 0.51 | 2.88 | 0.27 | 6.37 |
| Degradation rate | 94.72％ | 83.44％ | 90.89％ | 87.58％ | 97.01％ | 84.65％ |
Annotate:
Claims (2)
1. degrading pyrethroid pesticide residue bacterium, it is characterized in that, this microorganism is the bacterial strain LFJS3-9 of the Rhodopseudomonas (Pseudomonas sp.) of Gram-negative, be deposited at Chinese microorganism strain on October 12nd, 2005 and preserve management committee common micro-organisms center, culture presevation number is CGMCC NO.1477, and main biological characteristics is G
-, thalline is shaft-like, about 1.96 * 0.62 μ m of size extremely give birth to single flagellum; Nitrate reduction test, phenylalanine deaminase test, oxidase test, catalase test, 3-ketone group lactose mensuration, lipase test, denitrification test, the two hydrolysis experiment positives of arginine; It is negative that citrate test, gelatin liquification test, methyl red test, V.P. test, pyocyanin produce test, fluorochrome produces test; Can utilize glucose and fructose, can not hydrolyzed starch, produce alkali in the strain growth process, the Genbank number of landing of this bacterial strain 16S rDNA is DQ164217.
2. bacterium for degradating residual agricultural chemical of producing with the described degrading pyrethroid pesticide residue bacterium of claim 1 is to produce by the following method:
1) degrading pyrethroid pesticide residue bacterium LFJS3-9 test tube kind is inoculated in broth culture and shakes in the bottle, shaking culture is to logarithmic phase;
2) above-mentioned cultured bacterial classification is inoculated into 500 liters of seeding tanks by the inoculum size of the volume ratio 10% of seed tank culture base, be cultured to logarithmic phase, the used culture medium prescription of seeding tank is: glucose 0.8%, (NH
4)
2SO
41%, K
2HPO
40.2%, MgSO
40.05%, NaCl 0.01%, CaCO
30.3%, yeast extract paste 0.02%, pH value 7.2-7.5;
3) seed liquor is inserted to produce by 10% the inoculum size of producing jar substratum jar cultivate, it is identical with the seed tank culture base to produce jar used substratum;
4) air flow of sterile air is 1 in the culturing process of seeding tank and production jar: 0.6-1.2, stirring velocity is 180-240 rev/min, culture temperature is 30-35 ℃, the whole process incubation time is 48-60 hour, after the fermentation ends thalline quantity reach 1,000,000,000/more than the ml, fermentation is finished the back nutrient solution and is gone out jar and directly be distributed into liquid dosage form with plastic barrel or packing bottle or adopt peat to adsorb and be distributed into the solid fungicide formulation with packing bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100225487A CN100368528C (en) | 2005-12-23 | 2005-12-23 | Bacterium for degrading pyrethroid pesticide residue and produced bacterium formulation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100225487A CN100368528C (en) | 2005-12-23 | 2005-12-23 | Bacterium for degrading pyrethroid pesticide residue and produced bacterium formulation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1800354A CN1800354A (en) | 2006-07-12 |
| CN100368528C true CN100368528C (en) | 2008-02-13 |
Family
ID=36810568
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100225487A Expired - Fee Related CN100368528C (en) | 2005-12-23 | 2005-12-23 | Bacterium for degrading pyrethroid pesticide residue and produced bacterium formulation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100368528C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101724576B (en) * | 2008-10-21 | 2012-01-04 | 中国科学院生态环境研究中心 | Pyrethroid insecticide degradation bacteria and method for preparing fungicide thereof |
| CN102732450B (en) * | 2012-05-09 | 2013-05-01 | 浙江农林大学 | Phthalthrin degrading bacterium and application thereof |
| CN105903148B (en) * | 2016-05-24 | 2019-02-19 | 四川大学 | Can efficient degradation cypermethrin and 3- phenoxy benzoic acid wettable bacteria powder and preparation method |
| CN107541479B (en) * | 2017-09-20 | 2020-04-14 | 华南农业大学 | Insecticide-esfenpropathrin degrading strain, microbial inoculum and degrading process thereof |
| CN110591981B (en) * | 2019-10-24 | 2021-01-15 | 南京师范大学 | Paracoccus P-2 for degrading deltamethrin and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1302865A (en) * | 2001-01-17 | 2001-07-11 | 南京农业大学 | Bacterium for degradating residual agricultural organophosphorus chemical and its bacterial preparation |
-
2005
- 2005-12-23 CN CNB2005100225487A patent/CN100368528C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1302865A (en) * | 2001-01-17 | 2001-07-11 | 南京农业大学 | Bacterium for degradating residual agricultural organophosphorus chemical and its bacterial preparation |
Non-Patent Citations (2)
| Title |
|---|
| TRANSFORMATION OF SYNTHETIC PYRETHROIDINS ECTICIDES BY A THERMOPHILIC BACILLUS SP. MALONEY S E.ARCH MICROBIOL,Vol.15 No.8. 1992 * |
| 一株农药降解菌的分离与鉴定. 虞云龙等.华南理工大学学报（自然科学版），第24卷. 1996 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1800354A (en) | 2006-07-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102604859B (en) | Pyrethroid pesticide residue degradation bacteria as well as bacteria agent and application | |
| CN101338286B (en) | Chlorotoluron pesticide residue degradation strain and strain agent prepared by the strain | |
| CN100415872C (en) | DDT pesticide residue degradation bacterium and produced bacterium agent | |
| CN100368528C (en) | Bacterium for degrading pyrethroid pesticide residue and produced bacterium formulation | |
| CN101935627B (en) | Bromoxynil octanoate degrading bacteria and bacterial agent prepared from same | |
| CN100339473C (en) | Degradation bacteria for carbendazim pesticide residue and bacterial agent produced thereby | |
| CN105420172B (en) | Microbial inoculum and the application of a kind of metribuzin pesticide residual degrading bacteria and its production | |
| CN100371436C (en) | Bacterium for degrading chlorpyrifos pesticide residue and produced bacterium formulation | |
| CN1800353A (en) | Bacterium for degrading phoxim pesticide residue and produced bacterium formulation | |
| CN101974453B (en) | Pyrethroid pesticide degrading bacteria and bactericide thereof | |
| CN100596305C (en) | Atrazine herbicide degradation bacterium preparation making method | |
| CN103409336B (en) | Bulkholderia cepasea LH-ZY01 and the application in suppression dry thread Pyrenomycetes thereof | |
| CN100396772C (en) | Bacterium for degrading monocron pesticide residue and produced bacterium formulation | |
| CN101525584B (en) | Degradation bacteria for removing fomesafen pesticide residue and microbial inoculum prepared by same | |
| CN101560482B (en) | Buprofezin pesticide residual degrading bacteria and produced microbial inoculum thereof | |
| CN1302865A (en) | Bacterium for degradating residual agricultural organophosphorus chemical and its bacterial preparation | |
| CN1563356A (en) | Bacterium of degrading residual of organophorus pespared agent of bacterium | |
| CN1257265C (en) | Triazine herbicide residual degrading strain and strain therefrom | |
| CN102676439A (en) | Serratia strain and method for disposing methidathion pesticide residues on surfaces of soil and fruits/vegetables | |
| CN101486985B (en) | Nicosulfuron pesticide residue degrading bacterium and inocula produced therefrom | |
| CN101928687B (en) | Fluoroglycofen degrading bacteria and bacterial agent prepared from same | |
| CN101168731A (en) | Method for preparing methyl parathion degradation bacterium and enzyme preparation thereof | |
| CN100368529C (en) | Bacterium for degrading metsulfuron-methyl pesticide residue and produced bacterium formulation | |
| CN101348771B (en) | Imazethapyr pesticide residue degrading bacterial and inocula produced therefrom | |
| CN101402927B (en) | 2,4-dichlorobenzene oxygen butyl acetate pesticide residue degradation bacterium and produced bacterium agent thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Yixing Qiancheng Biological Co., Ltd. Assignor: Nanjing Agricultural University Contract record no.: 2010320000457 Denomination of invention: Bacterium for degrading pyrethroid pesticide residue and produced bacterium formulation Granted publication date: 20080213 License type: Exclusive License Open date: 20060712 Record date: 20100426 |
|
| EC01 | Cancellation of recordation of patent licensing contract |
Assignee: Yixing Qiancheng Biological Co., Ltd. Assignor: Nanjing Agricultural University Contract record no.: 2010320000457 Date of cancellation: 20110310 |
|
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Xuzhou Batian Ecology Co., Ltd. Assignor: Nanjing Agricultural University Contract record no.: 2011320000418 Denomination of invention: Bacterium for degrading pyrethroid pesticide residue and produced bacterium formulation Granted publication date: 20080213 License type: Exclusive License Open date: 20060712 Record date: 20110328 |
|
| EC01 | Cancellation of recordation of patent licensing contract |
Assignee: Xuzhou Batian Ecology Co., Ltd. Assignor: Nanjing Agricultural University Contract record no.: 2011320000418 Date of cancellation: 20120110 |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080213 Termination date: 20171223 |