CN107683335A - 用于核酸分离的自动化方法 - Google Patents
用于核酸分离的自动化方法 Download PDFInfo
- Publication number
- CN107683335A CN107683335A CN201680030749.8A CN201680030749A CN107683335A CN 107683335 A CN107683335 A CN 107683335A CN 201680030749 A CN201680030749 A CN 201680030749A CN 107683335 A CN107683335 A CN 107683335A
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- alcohol
- separation
- butanol
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims (15)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP15171261 | 2015-06-09 | ||
EP15171261.9 | 2015-06-09 | ||
PCT/EP2016/063150 WO2016198519A1 (en) | 2015-06-09 | 2016-06-09 | Automatable method for nucleic acid isolation |
Publications (1)
Publication Number | Publication Date |
---|---|
CN107683335A true CN107683335A (zh) | 2018-02-09 |
Family
ID=53373331
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201680030749.8A Pending CN107683335A (zh) | 2015-06-09 | 2016-06-09 | 用于核酸分离的自动化方法 |
Country Status (7)
Country | Link |
---|---|
US (1) | US10829758B2 (zh) |
EP (2) | EP4186980A1 (zh) |
JP (1) | JP6815334B2 (zh) |
CN (1) | CN107683335A (zh) |
CA (1) | CA2982706A1 (zh) |
ES (1) | ES2965460T3 (zh) |
WO (1) | WO2016198519A1 (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2900635T3 (es) | 2015-07-23 | 2022-03-17 | Biocartis Nv | Secuenciación optimizada de muestras clínicas |
ES2964100T3 (es) * | 2017-09-18 | 2024-04-04 | Phase Scient International Ltd | Método de utilización de un sistema acuoso bifásico para el aislamiento, purificación y/o concentración de fragmentos cortos de ácidos nucleicos |
CA3120216A1 (en) * | 2018-11-19 | 2020-05-28 | Biocartis Nv | Enhanced detection of low-copy-number nucleic acids in an integrated workflow |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004108925A1 (en) * | 2003-06-04 | 2004-12-16 | Qiagen As | Method for sequentially isolating dna and rna from the same nucleic acid-containing sample |
CN102046793A (zh) * | 2008-05-30 | 2011-05-04 | 恰根有限公司 | 用于分离和/或纯化核酸的裂解、结合和/或洗涤试剂 |
EP2345719A1 (en) * | 2010-01-18 | 2011-07-20 | Qiagen GmbH | Method for isolating small RNA |
WO2013024072A1 (en) * | 2011-08-12 | 2013-02-21 | Qiagen Gmbh | Method for isolating nucleic acids |
WO2014144174A1 (en) * | 2013-03-15 | 2014-09-18 | Abbott Molecular Inc. | Compositions and methods for nucleic acid extraction |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NL8900725A (nl) | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
DE19746874A1 (de) | 1997-10-23 | 1999-04-29 | Qiagen Gmbh | Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen |
GB2346615B (en) | 1998-11-17 | 2003-10-15 | Cambridge Molecular Tech | Isolating nucleic acid |
JP4907030B2 (ja) * | 2000-03-24 | 2012-03-28 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 分子の単離のための多孔性強磁性又はフェリ磁性ガラス粒子 |
JP2005137298A (ja) | 2003-11-07 | 2005-06-02 | Fuji Photo Film Co Ltd | 核酸の分離精製方法 |
EP1690938A1 (de) * | 2005-02-11 | 2006-08-16 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden |
ATE529186T1 (de) | 2005-06-30 | 2011-11-15 | Biocartis Sa | Kassette zur automatisierten medizinischen diagnose |
JP2007325562A (ja) | 2006-06-09 | 2007-12-20 | Fujifilm Corp | 核酸抽出法 |
EP2128169A1 (de) | 2008-05-30 | 2009-12-02 | Qiagen GmbH | Verfahren zur Isolierung von kurzkettigen Nukleinsäuren |
EP2264168B1 (de) * | 2009-06-18 | 2014-12-17 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren |
EP2770056B1 (en) * | 2012-07-18 | 2017-05-31 | Zymo Research Corporation | Nucleic acid purification |
-
2016
- 2016-06-09 CA CA2982706A patent/CA2982706A1/en active Pending
- 2016-06-09 WO PCT/EP2016/063150 patent/WO2016198519A1/en active Application Filing
- 2016-06-09 ES ES16732490T patent/ES2965460T3/es active Active
- 2016-06-09 EP EP22216565.6A patent/EP4186980A1/en not_active Withdrawn
- 2016-06-09 JP JP2017563581A patent/JP6815334B2/ja active Active
- 2016-06-09 EP EP16732490.4A patent/EP3307907B1/en active Active
- 2016-06-09 CN CN201680030749.8A patent/CN107683335A/zh active Pending
- 2016-06-09 US US15/570,947 patent/US10829758B2/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004108925A1 (en) * | 2003-06-04 | 2004-12-16 | Qiagen As | Method for sequentially isolating dna and rna from the same nucleic acid-containing sample |
CN102046793A (zh) * | 2008-05-30 | 2011-05-04 | 恰根有限公司 | 用于分离和/或纯化核酸的裂解、结合和/或洗涤试剂 |
EP2345719A1 (en) * | 2010-01-18 | 2011-07-20 | Qiagen GmbH | Method for isolating small RNA |
WO2013024072A1 (en) * | 2011-08-12 | 2013-02-21 | Qiagen Gmbh | Method for isolating nucleic acids |
WO2014144174A1 (en) * | 2013-03-15 | 2014-09-18 | Abbott Molecular Inc. | Compositions and methods for nucleic acid extraction |
Also Published As
Publication number | Publication date |
---|---|
US10829758B2 (en) | 2020-11-10 |
ES2965460T3 (es) | 2024-04-15 |
EP3307907A1 (en) | 2018-04-18 |
WO2016198519A1 (en) | 2016-12-15 |
CA2982706A1 (en) | 2016-12-15 |
EP3307907C0 (en) | 2023-11-15 |
JP2018518171A (ja) | 2018-07-12 |
JP6815334B2 (ja) | 2021-01-20 |
EP3307907B1 (en) | 2023-11-15 |
US20180291367A1 (en) | 2018-10-11 |
EP4186980A1 (en) | 2023-05-31 |
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Legal Events
Date | Code | Title | Description |
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PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
EE01 | Entry into force of recordation of patent licensing contract | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20180209 Assignee: Guangzhou Wanfu catis Biotechnology Co., Ltd Assignor: Wanfu Cadiz Co., Ltd Contract record no.: X2020990000658 Denomination of invention: Automated methods for nucleic acid separation License type: Common License Record date: 20201203 |
|
CB02 | Change of applicant information | ||
CB02 | Change of applicant information |
Address after: Mechelen Applicant after: Baiocatis biology Co.,Ltd. Address before: Mechelen Applicant before: BIOCARTIS N.V. |