CN107624651A - A kind of method of batch production Plant Tissue Breeding and the incubator for realizing this method - Google Patents
A kind of method of batch production Plant Tissue Breeding and the incubator for realizing this method Download PDFInfo
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- CN107624651A CN107624651A CN201711108205.1A CN201711108205A CN107624651A CN 107624651 A CN107624651 A CN 107624651A CN 201711108205 A CN201711108205 A CN 201711108205A CN 107624651 A CN107624651 A CN 107624651A
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- casing
- incubator
- tissue culture
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- plant tissue
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Abstract
The present invention relates to the method for Plant Tissue Breeding and the incubator for realizing this method.The method of Plant Tissue Breeding comprises the following steps：Tissue culture bottle to be cultivated is put in incubator, then the incubator equipped with tissue culture bottle is stacked in culturing room.Realizing the incubator of this method includes casing, the LED on edge, reflecting assembly, power supply module and power line on box inside, casing is using the plastic crate for having more than 30% ventilative glazed area, casing is provided centrally with reflecting assembly, the tissue culture bottle for needing to be cultivated is put around casing interior edge reflecting assembly, the incubator equipped with tissue culture bottle is stacked in culturing room.The present invention is simple and compact for structure, and culturing room's space availability ratio is high, and suitable for factorial praluction, the tissue culture frame pattern used relative to current factory reduces factory's cost, saves working hour, reduces tissue-cultured seedling listing cost.
Description
Technical field
The present invention relates to the side of field of plant tissue culture, especially a kind of Plant Tissue Breeding for factorial praluction
Method.
The invention further relates to a kind of culture apparatus, particularly a kind of Plant Tissue Breeding case for factorial praluction.
Background technology
Plant Tissue Breeding is quick indoors and amount reproduction plant seedling method.Plant Tissue Breeding is also known as plant
Clone, refer to by sterile working any organ, tissue or the cell of plant be inoculated in human configuration containing nutriment and
On the culture medium of the compositions such as plant growth regulating substance, cultured in vitro is carried out under manual control environment, makes its growth, differentiation shape
Into the process of intact plant.
In the Plant Tissue Breeding operation of factorial praluction at present to use tissue culture frame, mainstay is mostly to use general-purpose angle more
The shelf of steel plastic-spraying, appearance and size are 1250 × 500 × 2000mm, and 4-6 layers, every layer is installed 2-3 tissue culture lamp.Cultivation stage
Tissue culture bottle is seated in each layer frame.This standardization fixed bedstead pattern comes from tissue culture laboratory, has many convenient and practical
Advantage, such as good heat dissipation effect, in uniform light, inspection observation are convenient.But with the popularization of LED technology, occur special
The LED tissue culture special lamps of LED cold light source plant tissue culture special lamps or specific wavelength, instead of conventional day-light lamp, tissue culture ring
Border radiating pressure is reduced, but due to still adopting past bedstead pattern, low space utilization, while in tissue-cultured seedling large-scale production
In the case of, this bedstead pattern also highlights the problems such as tissue culture factory overall cost is high.On the other hand, in order to utilize upper bed
Tissue culture bottle is put or removed to frame, it is necessary to operating personnel climb up climbs down one by one, very inconvenient, so that upper strata bedstead is often vacant.Training
Support room bedstead to fix, dead angle is a lot of, and also the clean environment to culturing room causes many inconvenience.
As application number CN200920147137.4, CN201520227384.0, CN201620267196.5,
Some Chinese patents such as CN201620680227.X are directed to a kind of dedicated incubator of Plant Tissue Breeding, this incubator
Miniaturization culture space with some standalone features, in the hope of the indoor environment independent of place, but its thinking is still real to meet
Room work requirements, rather than factorial praluction are tested, is helpless to solve the problems, such as that batch production bedstead pattern is highlighted.
The content of the invention
The technical problem to be solved in the present invention is：Overcome deficiency in the prior art, there is provided a kind of batch production plant tissue
The incubator and cultural method of culture, in the culturing room of routine, using tissue culture box mode, make culture space miniaturization and packaging
Change, reduce tissue culture factory cost, save working hour, reduce tissue-cultured seedling listing cost.
The method for plant tissue culture that the present invention solves above-mentioned technical problem comprises the following steps：
1) incubator and its all component sterilization used Plant Tissue Breeding, are then assembled；
2) tissue culture bottle to be cultivated is put in the incubator assembled；
3) incubator equipped with tissue culture bottle is stacked in culturing room, a passage is left between every two row, then connected every
Individual incubator power supply；
4) temperature and humidity in culturing room is adjusted to required scope；
5) when subculture is transferred, incubator power supply is cut off one by one, and incubator is moved into purification edge of table, completes switch over operation；
6) after the completion of transferring, tissue culture case that new tissue culture bottle reinstalls former case or changes the outfit new, above-mentioned 3-5 steps are repeated；
7) when emptying culturing room, incubator power supply is cut off one by one, incubator is moved into purge chamber, removes culture box assembly,
Conventional cleaning and sterilization are carried out to incubator, it is standby.
The present invention is that incubator includes casing and light supply apparatus used by solving above-mentioned technical problem, and the casing includes
Anti-skid structure, enable the casing laminated multi-layer.
Specifically, the casing selects long 40-50cm × wide 30-35cm × high 20-30cm plastic crate so that the casing
With placement standard tissue culture bottle 9-25, or the volumes of standard can 24-30 only.
Further, the casing open topped, four sides are provided with multiple through holes so that the casing with more than 30% it is saturating
Gas glazed area.
Further, the anti-skid structure includes the tread plate located at the chimeric side of casing upper end and casing bottom surface
Line, when upper and lower two tanks are stacking, the bottom surface of upper box and the chimeric side of lower box coordinate, for realizing the folded of casing
Put.
The anti-skid structure can also include the groove on plastic crate bottom plate and the projection of plastic crate upper end, up and down
Two plastic crates stacked stack function by groove and raised cooperation, realization.
Specifically, the anti-skid structure cause casing empty van stack 20 layers or load tissue culture bottle after stack 10 layers it is still steady
It is fixed.
Further, the center of the casing is provided with reflecting assembly, and the tissue culture bottle for tissue cultures is put along reflecting assembly
It is placed in casing.
Specifically, the reflecting assembly is cylindrical shape, and the outer wall of reflecting assembly has reflectorized material.
Specifically, the cylindric reflecting assembly has the anti-of reflection function from long 60-70cm × wide 18-20cm one side
Tabula rasa curves tubular, and the reflective of reflective tube faces out.
Specifically, the distance between the reflecting assembly and cabinet wall are 10-15cm.
Preferably, the light supply apparatus is LED, and the upper edge of the LED and casing is fixed by binding band.
Specifically, the length range of the LED is 1-3m, and specific length is according to tissue culture thing to the intensity of illumination limit
Requirement depending on.
Further, the LED is closed by silica gel so that LED has water proofing property.
Further, the casing also includes the driving power and power line with LED band connection.
Further, the relative two side of the casing, which is provided with, is easy to the handle that casing moves up.
Further, the incubator laminated multi-layer equipped with blake bottle manages in cellar culture room.
The beneficial effects of the invention are as follows：1) present invention uses the tissue culture case of laminated multi-layer in culturing room, is meeting plant
On the premise of the basic condition of tissue cultures, culture space is miniaturized, an equal amount of culturing room adds the training of plant tissue
Amount is supported, saves culture space and corresponding temperature control cost；2) using the tissue culture case of the present invention, relative to culturing rack mould before
Formula, reduce the input of factory's fixed assets；3) it can save blake bottle using the present invention tissue culture case, operator and be placed in training
The operation of time, particularly culturing rack upper strata that blake bottle is fetched on frame and from culturing rack are supported, can operate and be reduced to from three people
One people, so as to improve operating efficiency.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the required accompanying drawing used in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the structural representation of Plant Tissue Breeding case of the present invention.
Fig. 2 is the flow chart of method for plant tissue culture of the present invention.
1- casings in figure, 2-LED light bar, 3- reflective tubes, 4- driving powers, 5- power lines, 6- through holes, 7- are fitted together to side, 8-
Handle.
Embodiment
Presently in connection with accompanying drawing and preferred embodiment, the present invention is further illustrated.These accompanying drawings are simplified signal
Figure, only illustrate the basic structure of the present invention in a schematic way, therefore it only shows the composition relevant with the present invention.
Embodiment 1
The method of Plant Tissue Breeding of the present invention mainly comprises the following steps：1) incubator for using Plant Tissue Breeding
And its all component sterilization, then assembled, wherein the power supply module in component or plug connector use not in cleaning
The disinfectant of electric leakage can be caused；2) tissue culture bottle to be cultivated is put in the incubator assembled；3) by the training equipped with tissue culture bottle
Foster case is stacked in culturing room, and a passage is left between every two row, and the spacing of the interchannel is 60cm, then connects each training
Support case power supply；4) temperature and humidity in culturing room is adjusted to required scope；5) when subculture is transferred, incubator is cut off one by one
Power supply, incubator is moved into purification edge of table, completes switch over operation；6) after the completion of transferring, new tissue culture bottle reinstalls former case or changed the outfit new
Tissue culture case, repeat above-mentioned 3-5 steps.
Fig. 1 shows the incubator of Plant Tissue Breeding of the present invention.As shown in figure 1, the incubator include casing (1),
The LED (2) on edge, reflective tube (3), driving power (4) and power line (5) on box inside.
The casing (1) selects long by 44 × wide by 32 × high 25cm plastic crate, and the plastic crate is molded using flastic molding,
Casing (1) open topped, four sides are provided with multiple through holes (6) that casing (1) can be made to reach 40% ventilative glazed area.
The casing (1) includes anti-skid structure, and the anti-skid structure includes the chimeric side (7) of casing (1) upper end, described
Chimeric side (7) can coordinate with the bottom surface of casing (1) so that the casing (1) being capable of laminated multi-layer.
The relative two side of the casing (1), which is provided with, is easy to the handle (8) that casing (1) moves up, and is easy to turning for casing (1)
Fortune.
The length of the LED (2) is 2 meters, along arrangement, LED on inner side of the LED (2) along casing (1)
Band (2) is fixed with casing (1) by white binding band.
The LED (2) is closed by silica gel so that LED has water proofing property.
The reflective tube (3) is arranged on the center of casing (1), is placed with around casing (1) the interior edge reflective tube (3)
20 tissue culture bottles to be cultivated.
The reflective tube (3) is curved by 20 × 60cm rectangular reflective plate, and the reflector is that one side has reflection function
Lightweight thin sheet material, reflective the facing out of the reflective tube (3), distance is between the periphery of reflective tube (3) and casing (1) wall
15cm。
The driving power (4) and power line (5) are located at the outer wall of casing (1), driving power (4), power line (5) and case
Body (1) is fixed by white binding band.
When in use, the incubator equipped with tissue culture bottle is moved into culturing room, by incubator mounted on top, every two row stays one
60cm or so passage, often arrange incubator and include 10 casees incubators；Power line is connected to supply lines above the incubator stacked
Road.
Embodiment 2
The method of Plant Tissue Breeding of the present invention mainly comprises the following steps：1) incubator for using Plant Tissue Breeding
And its all component sterilization, then assembled, wherein the power supply module in component or plug connector use not in cleaning
The disinfectant of electric leakage can be caused；2) tissue culture bottle to be cultivated is put in the incubator assembled；3) by the training equipped with tissue culture bottle
Foster case is stacked in culturing room, and a passage is left between every two row, and the spacing of the interchannel is 70cm, then connects each training
Support case power supply；4) temperature and humidity in culturing room is adjusted to required scope；5) when subculture is transferred, incubator is cut off one by one
Power supply, incubator is moved into purification edge of table, completes switch over operation；6) when emptying culturing room, incubator power supply is cut off one by one, will be trained
Foster case moves to purge chamber, removes culture box assembly, and conventional cleaning and sterilization are carried out to incubator, standby.
The incubator can laminated multi-layer, including casing (1), the LED (2) on edge, reflective on box inside
Cylinder (3), driving power (4) and power line (5).
The casing (1) selects long by 44 × wide by 32 × high 25cm plastic crate, and the plastic crate is molded using flastic molding,
Casing (1) open topped, four sides are provided with multiple through holes (6) that casing (1) can be made to reach more than 30% ventilative glazed area.
The casing (1) includes anti-skid structure, and the anti-skid structure includes the groove and casing (1) of casing (1) bottom surface
The projection of upper end, when upper and lower two tanks stack, the groove of upper box can be with the male cooperation of lower box so that the casing (1)
Being capable of laminated multi-layer.
The relative two side of the casing (1), which is provided with, is easy to the handle (8) that casing (1) moves up, and is easy to turning for casing (1)
Fortune.
The length of the LED (2) is 3 meters, along arrangement, LED on inner side of the LED (2) along casing (1)
Band (2) is fixed with casing (1) by white binding band.
The LED (2) is closed by silica gel so that LED has water proofing property.
The reflective tube (3) is arranged on the center of casing (1), is placed with around casing (1) the interior edge reflective tube (3)
20 tissue culture bottles to be cultivated.
The reflective tube (3) is curved by 20 × 60cm rectangular reflective plate, and the reflector is that one side has reflection function
Lightweight thin sheet material, reflective the facing out of the reflective tube (3), distance is between the periphery of reflective tube (3) and casing (1) wall
15cm。
The driving power (4) and power line (5) are located at the outer wall of casing (1), driving power (4), power line (5) and case
Body (1) is fixed by white binding band.
When in use, the incubator equipped with tissue culture bottle is moved into culturing room, by incubator mounted on top, every two row stays one
70cm passage, often arrange incubator and include 10 casees incubators；Power line is connected to supply line above the incubator stacked.
The present invention is used for the tissue cultures of plant using the incubator of laminated multi-layer, makes the space packaging of Plant Tissue Breeding
Change, an equal amount of culturing room adds the culture amount of plant tissue, saves culture space and corresponding electric cost, be suitable to
Factorial praluction；Culturing rack is replaced using the incubator of the present invention, reduces the input of early stage tissue culture factory fixed assets, simultaneously
For operator, the time that blake bottle is placed on culturing rack and blake bottle is fetched from culturing rack can be saved, particularly
The operation on culturing rack upper strata, so as to improve operating efficiency.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art
Scholar can understand present disclosure and be carried out, and it is not intended to limit the scope of the present invention, all according to the present invention
The equivalent change or modification that Spirit Essence is made, it should all cover within the scope of the present invention.
Claims (10)
- A kind of 1. method of batch production Plant Tissue Breeding, it is characterised in that the method for plant tissue culture, be by following step It is rapid to complete：1) incubator and its all component sterilization used Plant Tissue Breeding, are then assembled；2) tissue culture bottle to be cultivated is put in the incubator assembled；3) incubator equipped with tissue culture bottle is stacked in culturing room, a passage is left between every two row, then connect each training Support case power supply；4) temperature and humidity in culturing room is adjusted to required scope；5) when subculture is transferred, incubator power supply is cut off one by one, and incubator is moved into transfer room purification edge of table, completes switch over operation.
- 2. a kind of incubator for being used to realize batch production method for plant tissue culture described in claim 1, including casing and light source Device, it is characterised in that：The casing can laminated multi-layer, the center of casing is provided with reflecting assembly, the group for tissue cultures Training bottle is placed in casing along reflecting assembly, and the light supply apparatus is arranged at edge on the inner side of casing.
- 3. incubator according to claim 2, it is characterised in that：The casing open topped, the side of casing four are provided with multiple Through hole.
- 4. incubator according to claim 2, it is characterised in that：The light supply apparatus is LED, the LED Fixed with the upper edge of casing by binding band.
- 5. incubator according to claim 4, it is characterised in that：The length of the LED can be adjusted, LED Length be adapted with requirement of the tissue culture crop to the intensity of illumination limit.
- 6. incubator according to claim 4, it is characterised in that：The LED is closed by silica gel.
- 7. the incubator according to claim 2 or 4, it is characterised in that：The casing also includes the drive with LED band connection Dynamic power supply and power line.
- 8. incubator according to claim 2, it is characterised in that：The reflecting assembly is cylindric, outside reflecting assembly Wall has reflectorized material.
- 9. incubator according to claim 2, it is characterised in that：The upper end of the casing is provided with chimeric side, casing Bottom surface is provided with skid resistance strip, and when upper and lower two tanks are stacking, the bottom surface of upper box and the chimeric side of lower box coordinate, and use In realizing stacking for casing.
- 10. incubator according to claim 2, it is characterised in that：The relative two side of the casing, which is provided with, is easy to casing The handle moved up.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711108205.1A CN107624651A (en) | 2017-11-08 | 2017-11-08 | A kind of method of batch production Plant Tissue Breeding and the incubator for realizing this method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711108205.1A CN107624651A (en) | 2017-11-08 | 2017-11-08 | A kind of method of batch production Plant Tissue Breeding and the incubator for realizing this method |
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| Publication Number | Publication Date |
|---|---|
| CN107624651A true CN107624651A (en) | 2018-01-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711108205.1A Withdrawn CN107624651A (en) | 2017-11-08 | 2017-11-08 | A kind of method of batch production Plant Tissue Breeding and the incubator for realizing this method |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201571391U (en) * | 2009-05-08 | 2010-09-08 | 陈吉宗 | Device for cultivating plant tissue |
| CN103907531A (en) * | 2013-01-06 | 2014-07-09 | 张征 | Plant tissue culture production process |
| CN204047473U (en) * | 2014-10-14 | 2014-12-31 | 淮北师范大学 | A kind of incubator for tissue culture of Plant Tissue Breeding |
| CN205113927U (en) * | 2015-11-10 | 2016-03-30 | 台州市黄岩迈斯特模塑有限公司 | Turnover basket |
| CN205946827U (en) * | 2016-07-01 | 2017-02-15 | 武汉伯远生物科技有限公司 | Energy -efficient multifunctional plant tissue culture case |
-
2017
- 2017-11-08 CN CN201711108205.1A patent/CN107624651A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201571391U (en) * | 2009-05-08 | 2010-09-08 | 陈吉宗 | Device for cultivating plant tissue |
| CN103907531A (en) * | 2013-01-06 | 2014-07-09 | 张征 | Plant tissue culture production process |
| CN204047473U (en) * | 2014-10-14 | 2014-12-31 | 淮北师范大学 | A kind of incubator for tissue culture of Plant Tissue Breeding |
| CN205113927U (en) * | 2015-11-10 | 2016-03-30 | 台州市黄岩迈斯特模塑有限公司 | Turnover basket |
| CN205946827U (en) * | 2016-07-01 | 2017-02-15 | 武汉伯远生物科技有限公司 | Energy -efficient multifunctional plant tissue culture case |
Non-Patent Citations (1)
| Title |
|---|
| 王三根: "《植物生理生化》", 31 January 2008 * |
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Application publication date: 20180126 |