CN104312901A - Microalgae cultivation module and large-scale microalgae factory cultivation system - Google Patents
Microalgae cultivation module and large-scale microalgae factory cultivation system Download PDFInfo
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- CN104312901A CN104312901A CN201410557127.3A CN201410557127A CN104312901A CN 104312901 A CN104312901 A CN 104312901A CN 201410557127 A CN201410557127 A CN 201410557127A CN 104312901 A CN104312901 A CN 104312901A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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Abstract
The invention relates to a microalgae cultivation module and a large-scale microalgae factory cultivation system. The microalgae cultivation module comprises a box-type housing, wherein a culture solution leading-in opening is formed in the box-type housing, and at least one externally connected carbon dioxide vent tube with a plurality of vent holes is formed in the bottom of a cavity of the box-type housing; the side wall of the lower part of the box-type housing is connected with a collection port for collecting an algae liquid in the box-type housing, and at least one stirring device arranged in a culture solution is arranged in the box-type housing; a plurality of LED (Light Emitting Diode) lamps for lighting are also arranged in the box-type housing. The large-scale microalgae factory cultivation system mainly comprises at least two microalgae cultivation modules, a solution replenishment mechanism, a carbon dioxide supply mechanism, a temperature control device and a PH adjustment mechanism, and is characterized in that the microalgae cultivation modules are connected in a parallel, serial or combination manner. The microalgae cultivation module and the large-scale microalgae factory cultivation system provided by the invention have the characteristics of simple structure, convenience in operation and cleaning, controllable culture conditions, large culture volume per unit of land and the like.
Description
Technical field
The present invention relates to a kind of microdisk electrode module and the large-scale industrialized culture systems of micro-algae, belong to microdisk electrode bioreactor technical field.
Technical background
Micro-algae is the biology earth occurred the earliest, and their individualities are small, from several microns to tens microns not etc., generally will just can observe under the microscope.Micro-algae abundant species, reproduction speed be fast, be rich in nutritive substance and various biologically active substance, and it is short to be easy to artificial propagation, breeding cycle, therefore, they have broad application prospects in food, medicine, healthcare products, makeup, aquaculture bait, fodder additives, biological pesticide, chemical industry, environmental protection etc.
In recent years, microalgae industryization is cultivated and is risen gradually, and the particularly cultivation of the micro-algae of some high added values, as salt algae, spirulina, Haematocoocus Pluvialls etc., the micro-algae of some of them is applicable to outdoor open cultivation, and other are then more suitable for closed cultivation.At present, most of closed reactor belongs to flats or tubulose is laid on indoor or outdoors, and their floor spaces are large, and land utilization ratio is low, and handling cost is high, and substantial contribution is wasted in infrastructure construction, causes microdisk electrode cost significantly to promote.
Summary of the invention
The object of this is to overcome the deficiency that prior art exists, and provide that a kind of structure is simple, operation and easy to clean, culture condition can be controlled, microdisk electrode module that land area of one unit culture volume is large and the large-scale industrialized culture systems of micro-algae.
The object of the invention is to have come by following technical solution, a kind of microdisk electrode module, it comprises a box-type shell, this box-type shell offers nutrient solution introducing port, the cavity bottom of box-type shell be at least provided with one that outreach, with the carbonic acid gas ventpipe of multiple production well, the lower sides of box-type shell is connected at least one collection interface for collecting algae liquid in box-type shell; At least one whipping appts being placed in nutrient solution is installed in described box-type shell, separately multiple LED for illumination is also installed in box-type shell.
Described box-type shell is a rectangular parallelepiped, is provided with multiple ultraviolet lamp for sterilization in this rectangular parallelepiped, and the mounting height of this ultraviolet lamp be positioned at box-type shell inner chamber top, the most high liquid level (HLL) that adds higher than nutrient solution; Described LED is under being arranged in most high liquid level (HLL) that nutrient solution adds and the water-proof LED lamp be immersed in nutrient solution.
A kind of large-scale industrialized culture systems of micro-algae formed with above-mentioned microdisk electrode block combiner, it mainly comprises: at least two microdisk electrode modules, a set of fluid infusion mechanism, carbonic acid gas feed mechanism, temperature control unit, PH regulating mechanism, described microdisk electrode module by and connect, series winding or mixed connection mode combine; Pond is prepared by external nutrient solution by described fluid infusion mechanism or preparing tank, well heater, liquid supplementation channel, trophic analysis device and water pump form, and liquid supplementation channel is wherein connected to the nutrient solution introducing port on each box-type shell by valve;
Described fluid infusion mechanism is configured with temperature control unit, and described temperature control unit is made up of indoor refrigeration system, indoor heating system, well heater, heating rod, thermometer, and carries out temperature adjusting by described temperature control unit to the nutrient solution in described fluid infusion mechanism;
Described carbonic acid gas feed mechanism is by carbon dioxide air source, pipe connecting, breather line and flow director form, and the breather line in each box-type shell by being connected to a total pipe connecting respectively by air valve, and connects carbon dioxide air source under the control of flow director;
Described carbonic acid gas feed mechanism is configured with and is placed in pH meter in each box-type shell, and measured the pH value of nutrient solution in each box-type shell by respective pH meter, by strengthening carbonic acid gas intake or reducing the pH value that carbonic acid gas intake realizes nutrient solution.
The LED illumination intensity control switches installed in each box-type shell of the present invention controls the intensity of illumination of long light wave and short light wave LED, controls to use long light wave LED or short light wave LED, and form illumination controlling organization by illumination type of switch;
The whipping appts installed in described each box-type shell is made up of motor, rotational speed governor, support and stirring turbine, is placed in the nutrient solution of box-type shell inner chamber by the stirring turbine of driven by motor;
The nutrient solution preparation pond of described fluid infusion mechanism or batch tank, its shape is square, rectangular parallelepiped, right cylinder, cylindroid;
Indoor heating in described temperature control unit and refrigeration system, optionally hotwork be winter thermal source, underground water supplements as low-temperature receiver in summer.
Box-type shell of the present invention is the cuboid container of long 3-20m, wide 0.3-1.0m, high 1-5m; This cuboid container is made up of transparent or opaque PC, PVC, PE, PETG, glass, glass reinforced plastic, synthetic glass, steel plate, bamboo plank, stainless steel plate material.
Described box-type shell is made according to opaque material, posts one deck tapetum lucidum at this opaque material inwall.
The present invention has that structure is simple, operation and easy to clean, can control culture condition, the feature such as land area of one unit culture volume is large.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of microdisk electrode module of the present invention.
Fig. 2 be microdisk electrode module of the present invention and connect unitized construction schematic diagram.
Fig. 3 is the series combination structural representation of microdisk electrode module of the present invention.
Fig. 4 is the mixed connection unitized construction schematic diagram of microdisk electrode module of the present invention.
Label in figure is: 1-algae liquid collects mouth, 2-carbonic acid gas ventpipe, 3-nutrient solution introducing port, 4-LED lamp, 5-ultraviolet lamp, 6-whipping appts, 7-box-type shell.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail.Shown in Fig. 1, a kind of microdisk electrode module of the present invention, it comprises a box-type shell 7, this box-type shell 7 offers nutrient solution introducing port 3, the cavity bottom of box-type shell 7 be at least provided with one that outreach, with the carbonic acid gas ventpipe 2 of multiple production well, the lower sides of box-type shell 7 is connected at least one collection mouth 1 for collecting algae liquid in box-type shell; At least one whipping appts 6 being placed in nutrient solution is installed in described box-type shell 7, multiple LED 4 for illumination is separately also installed in box-type shell 7.
Shown in figure, described box-type shell 7 is a rectangular parallelepiped, is provided with multiple ultraviolet lamp 5 for sterilization inside and outside this rectangular parallelepiped, and the mounting height of this ultraviolet lamp 5 be positioned at box-type shell 7 inner chamber top, the most high liquid level (HLL) that adds higher than nutrient solution; Described LED 4 is under the most high liquid level (HLL) that is arranged in nutrient solution and adds and the water-proof LED lamp be immersed in nutrient solution.
Shown in Fig. 2,3,4, a kind of large-scale industrialized culture systems of micro-algae formed with described microdisk electrode block combiner, it mainly comprises: at least two microdisk electrode modules, a set of fluid infusion mechanism, carbonic acid gas feed mechanism, temperature control unit, PH regulating mechanism, described microdisk electrode module by and connect, series winding or mixed connection mode combine; Pond is prepared by external nutrient solution by described fluid infusion mechanism or preparing tank, well heater, liquid supplementation channel, trophic analysis device and water pump form, and liquid supplementation channel is wherein connected to the nutrient solution introducing port on each box-type shell by valve;
Described fluid infusion mechanism is configured with temperature control unit, and described temperature control unit is made up of indoor refrigeration system, indoor heating system, well heater, heating rod, thermometer, and carries out temperature adjusting by described temperature control unit to the nutrient solution in described fluid infusion mechanism;
Described carbonic acid gas feed mechanism is by carbon dioxide air source, pipe connecting, ventpipe and flow director form, and the ventpipe in each box-type shell by being connected to a total pipe connecting respectively by air valve, and connects carbon dioxide air source under the control of flow director;
Described carbonic acid gas feed mechanism is configured with and is placed in pH meter in each box-type shell, and measured the pH value of nutrient solution in each box-type shell by respective pH meter, by strengthening carbonic acid gas intake or reducing the pH value that carbonic acid gas intake realizes nutrient solution.
The LED illumination intensity control switches installed in described each box-type shell controls the intensity of illumination of long light wave and short light wave LED, controls to use long light wave LED or short light wave LED, and form illumination controlling organization by illumination type of switch;
The whipping appts installed in described each box-type shell is made up of motor, rotational speed governor, support and stirring turbine, is placed in the nutrient solution of box-type shell inner chamber by the stirring turbine of driven by motor;
The nutrient solution preparation pond of described fluid infusion mechanism or batch tank, its shape is square, rectangular parallelepiped, right cylinder, cylindroid;
Indoor heating in described temperature control unit and refrigeration system, optionally hotwork be winter thermal source, underground water supplements as low-temperature receiver in summer.
The preferred embodiment of the present invention is: described box-type shell is the cuboid container of long 3-20m, wide 0.3-1.0m, high 1-5m; This cuboid container is made up of transparent or opaque PC, PVC, PE, PETG, glass, glass reinforced plastic, synthetic glass, steel plate, bamboo plank, stainless steel plate material.
Box-type shell of the present invention is made according to opaque material, posts one deck tapetum lucidum at this opaque material inwall.
Embodiment: a kind of microdisk electrode module provided by the invention, it mainly comprises:
One microdisk electrode module, its long 3-20m, wide 0.3-1m, high 1-5m.
One fluid infusion mechanism, prepares pond by nutrient solution or preparing tank, well heater, liquid supplementation channel, trophic analysis device and water pump form, and liquid supplementation channel is connected with nutrient solution introducing port.
One carbonic acid gas feed mechanism, is made up of carbon dioxide air source, breather line and flow director, and breather line is arranged on cultivation module bottom, and it has production well, and carbonic acid gas enters nutrient solution through source of the gas, flow director, breather line and production well.
One illumination controlling organization, by the LED of different wave length, intensity of illumination trip switch and illumination type trip switch composition.
One temperature control unit, is made up of indoor refrigeration system, indoor heating system, well heater, heating rod, thermometer etc.
One PH regulating mechanism, is counted by PH and CO2 flow controller forms, by the pH value regulating the amount adding carbonic acid gas to control micro algae culturing liquid.
One stirring mechanism, is made up of motor, rotational speed governor, support, stirring turbine, and stir turbine and stir algae liquid under driven by motor, a stirring system can install multiple stirring turbine, and the width stirring turbine is 0.2-0.8m.
Described microdisk electrode module housing can be made with transparent or opaque material, as PC, PVC, PE, PETG, glass, glass reinforced plastic, synthetic glass, steel plate, bamboo plank, stainless steel plate etc., if make of opaque material, one deck tapetum lucidum can be pasted, as aluminium foil etc. at cultivation module inwall.
The light source of described illumination system directly contacts with nutrient solution, is the LED of waterproof.
Heating rod in described temperature control system, available bamboo carbon fibre is as heat-conduction medium, and indoor heating and refrigeration system, can optionally hotwork be thermal source (winter), underground water supplements as low-temperature receiver (summer).
Described microdisk electrode factory by several microdisk electrode modules through and connect, contact or the mode of mixed connection combines, each cultivates the fluid infusion mouth of module, carbonic acid gas introducing port, algae liquid collection mouth are connected respectively.
The factory building of described microdisk electrode factory, fitted up formed by insulation, lagging material, its size is determined as required.Factory building can with steel construction, bamboo-wood structure, concrete structure, mould timber structure etc. and build.
In said structure, the quantity of microdisk electrode module and large I are determined according to the design of microdisk electrode factory, and carbonic acid gas air flow is adjusting size needed for microdisk electrode; The optical wavelength of LED needed for microdisk electrode process and light intensity are installed, basic index longwave optical in vegetative period, exponential growth later stage short wavelength light.
The specific works process of microdisk electrode module of the present invention is: the size and number determining microdisk electrode module according to actual needs, installs microdisk electrode module, sets up microdisk electrode factory.Close algae liquid and collect mouth 1, open ultraviolet lamp 5, sterilization 20min; Close ultraviolet lamp 5, the nutrient solution prepared is imported microdisk electrode module through nutrient solution introducing port 3; Algae kind is accessed microdisk electrode module by nutrient solution introducing port 3; Open longwave optical (about 640nm) LED 4, the light intensity needed for different algae kind opens the LED 4 of different quantities; Connect carbonic acid gas ventpipe 2, pass into carbonic acid gas; Open motor and stir algae liquid, start to cultivate; In culturing process, can according to practical situation by nutrient solution introducing port 3 Additional nutrient solution, by the temperature of thermometer measure nutrient solution, algae liquid temp is controlled by refrigeration system in factory when temperature is higher, nutrient solution can be preheated when temperature is lower then nutrient solution is poured into cultivation module or heating rod raising culture temperature is installed, with the pH value of PH measurement amount nutrient solution, when pH value is higher, strengthen carbonic acid gas intake, otherwise, reduce carbonic acid gas intake; After cultivation completes, open algae liquid and collect interface 1, collect algae liquid.
Claims (6)
1. a microdisk electrode module, it comprises a box-type shell, it is characterized in that: on this box-type shell, offer nutrient solution introducing port, the cavity bottom of box-type shell be at least provided with one that outreach, with the carbonic acid gas ventpipe of multiple production well, the lower sides of box-type shell is connected at least one collection interface for collecting algae liquid in box-type shell; At least one whipping appts being placed in nutrient solution is installed in described box-type shell, separately multiple LED for illumination is also installed in box-type shell.
2. microdisk electrode module according to claim 1, it is characterized in that: described box-type shell is a rectangular parallelepiped, multiple ultraviolet lamp for sterilization is installed in this rectangular parallelepiped, and the mounting height of this ultraviolet lamp be positioned at box-type shell inner chamber top, the most high liquid level (HLL) that adds higher than nutrient solution; Described LED is under being arranged in most high liquid level (HLL) that nutrient solution adds and the water-proof LED lamp be immersed in nutrient solution.
3. the large-scale industrialized culture systems of micro-algae formed with microdisk electrode block combiner described in claim 1 or 2, it mainly comprises: at least two microdisk electrode modules, a set of fluid infusion mechanism, carbonic acid gas feed mechanism, temperature control unit, PH regulating mechanism, it is characterized in that described microdisk electrode module by and connect, series winding or mixed connection mode combine; Pond is prepared by external nutrient solution by described fluid infusion mechanism or preparing tank, well heater, liquid supplementation channel, trophic analysis device and water pump form, and liquid supplementation channel is wherein connected to the nutrient solution introducing port on each box-type shell by valve;
Described fluid infusion mechanism is configured with temperature control unit, and described temperature control unit is made up of indoor refrigeration system, indoor heating system, well heater, heating rod, thermometer, and carries out temperature adjusting by described temperature control unit to the nutrient solution in described fluid infusion mechanism;
Described carbonic acid gas feed mechanism is by carbon dioxide air source, pipe connecting, breather line and flow director form, and the breather line in each box-type shell by being connected to a total pipe connecting respectively by air valve, and connects carbon dioxide air source under the control of flow director;
Described carbonic acid gas feed mechanism is configured with and is placed in pH meter in each box-type shell, and measured the pH value of nutrient solution in each box-type shell by respective pH meter, by strengthening carbonic acid gas intake or reducing the pH value that carbonic acid gas intake realizes nutrient solution.
4. the large-scale industrialized culture systems of micro-algae according to claim 3, it is characterized in that the LED illumination intensity control switches installed in described each box-type shell controls the intensity of illumination of long light wave and short light wave LED, control to use long light wave LED or short light wave LED by illumination type of switch, and form illumination controlling organization;
The whipping appts installed in described each box-type shell is made up of motor, rotational speed governor, support and stirring turbine, is placed in the nutrient solution of box-type shell inner chamber by the stirring turbine of driven by motor;
The nutrient solution preparation pond of described fluid infusion mechanism or batch tank, its shape is square, rectangular parallelepiped, right cylinder, cylindroid;
Indoor heating in described temperature control unit and refrigeration system, optionally hotwork be winter thermal source, underground water supplements as low-temperature receiver in summer.
5. the large-scale industrialized culture systems of micro-algae according to claim 3 or 4, is characterized in that described box-type shell is the cuboid container of long 3-20m, wide 0.3-1.0m, high 1-5m; This cuboid container is made up of transparent or opaque PC, PVC, PE, PETG, glass, glass reinforced plastic, synthetic glass, steel plate, bamboo plank, stainless steel plate material.
6. the large-scale industrialized culture systems of micro-algae according to claim 5, is characterized in that described box-type shell adopts opaque material to make, posts one deck tapetum lucidum at this opaque material inwall.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105754843A (en) * | 2016-05-10 | 2016-07-13 | 中国科学院青岛生物能源与过程研究所 | Relatively enclosed multi-thin layer adherent culture device for production of microalgae |
| CN105950441A (en) * | 2016-03-28 | 2016-09-21 | 青岛农业大学 | Microalgae large-scale culture device suitable for improving carbon fixation efficiency |
| CN106367313A (en) * | 2016-08-26 | 2017-02-01 | 浙江天地环保科技有限公司 | Flat plate photobioreactor |
| CN106754342A (en) * | 2017-01-17 | 2017-05-31 | 杭州元点生物科技有限公司 | A kind of equipment of the culture spirulina of high-purity indoors of closed |
| CN107083322A (en) * | 2017-06-23 | 2017-08-22 | 四川省天惠能源科技有限公司 | A kind of multilayer micro algae growth equipment for breeding |
| CN110387315A (en) * | 2019-08-27 | 2019-10-29 | 济宁学院 | Microalgae adsorbs the device and method of carbon dioxide preparation biodiesel |
| CN111411044A (en) * | 2020-03-31 | 2020-07-14 | 广东海兴农集团有限公司 | Industrial bait microalgae culture method |
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| CN103283582A (en) * | 2012-02-23 | 2013-09-11 | 朴胜敏 | Photo biological algae cultivator for maximizing carbon dioxide reduction rate, and method for producing algae by using the same |
| CN103525686A (en) * | 2013-10-24 | 2014-01-22 | 重庆大学 | Composite reinforced microalgae photobioreactor based on hollow light pipes |
| CN104073435A (en) * | 2014-07-02 | 2014-10-01 | 杭州鑫伟低碳技术研发有限公司 | Movable all-weather microalgae light complement bioreaction culture system and culture method |
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| CN102803463A (en) * | 2009-05-06 | 2012-11-28 | 生物进程H2O有限责任公司 | Systems, apparatuses and methods for cultivating microorganisms and mitigation of gases |
| CN103283582A (en) * | 2012-02-23 | 2013-09-11 | 朴胜敏 | Photo biological algae cultivator for maximizing carbon dioxide reduction rate, and method for producing algae by using the same |
| CN103525686A (en) * | 2013-10-24 | 2014-01-22 | 重庆大学 | Composite reinforced microalgae photobioreactor based on hollow light pipes |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105950441A (en) * | 2016-03-28 | 2016-09-21 | 青岛农业大学 | Microalgae large-scale culture device suitable for improving carbon fixation efficiency |
| CN105754843A (en) * | 2016-05-10 | 2016-07-13 | 中国科学院青岛生物能源与过程研究所 | Relatively enclosed multi-thin layer adherent culture device for production of microalgae |
| CN106367313A (en) * | 2016-08-26 | 2017-02-01 | 浙江天地环保科技有限公司 | Flat plate photobioreactor |
| CN106754342A (en) * | 2017-01-17 | 2017-05-31 | 杭州元点生物科技有限公司 | A kind of equipment of the culture spirulina of high-purity indoors of closed |
| CN107083322A (en) * | 2017-06-23 | 2017-08-22 | 四川省天惠能源科技有限公司 | A kind of multilayer micro algae growth equipment for breeding |
| CN110387315A (en) * | 2019-08-27 | 2019-10-29 | 济宁学院 | Microalgae adsorbs the device and method of carbon dioxide preparation biodiesel |
| CN111411044A (en) * | 2020-03-31 | 2020-07-14 | 广东海兴农集团有限公司 | Industrial bait microalgae culture method |
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Application publication date: 20150128 |