CN107613162B

CN107613162B - Portable micro-fluidic chip imaging system

Info

Publication number: CN107613162B
Application number: CN201710683123.3A
Authority: CN
Inventors: 王敏; 吴非逸
Original assignee: Zhejiang University ZJU
Current assignee: Zhejiang University ZJU
Priority date: 2017-08-11
Filing date: 2017-08-11
Publication date: 2020-11-20
Anticipated expiration: 2037-08-11
Also published as: CN107613162A

Abstract

The invention discloses a portable micro-fluidic chip imaging system, which ensures the consistency of the height, angle and illumination condition of each shooting when a reaction result in a micro-fluidic chip is obtained by a mobile phone, has low requirement on the external environment, greatly improves the accuracy of a reading result, has good repeatability and satisfactory effect, is convenient to carry, and is very suitable for micro-fluidic chip imaging. The imaging system is small in size and convenient to carry, the imaging system cannot be in direct contact with a reaction area in the microfluidic chip, the interior of the imaging system cannot be polluted by chemical reagents, the cleaning trouble is solved, the potential safety hazard is reduced, and the equipment does not have obvious vibration as a scanner during imaging, so that the imaging quality is higher.

Description

Portable micro-fluidic chip imaging system

Technical Field

The invention relates to a micro-fluidic chip imaging system, and belongs to the field of micro-fluidic.

Technical Field

The micro-fluidic chip is an integratable technical platform, is one of research hotspots in the field of analytical chemistry at present, integrates chemical and biological analysis processes on one chip, has the characteristics of low cost and high sensitivity, and has great potential in the aspects of biosensing, cell screening, gene analysis, drug delivery, disease diagnosis, environmental monitoring and the like.

Reaction results in microfluidic chips often need to be captured by a mobile phone, a scanner, or the like. The scanner is relatively big, and is not convenient for carry to the inside inevitable reaction zone direct contact with among the micro-fluidic chip of scanner when formation of image, this will lead to inside being polluted by chemical reagent of scanner, both brought abluent puzzlement, also cause the potential safety hazard easily. In addition, the scanner vibrates significantly during imaging, thereby degrading the quality of the image. When the reaction result in the microfluidic chip is obtained through the mobile phone, the height, the angle and the illumination condition of each shooting are difficult to keep consistent, so that the repeatability of the captured result is poor. Therefore, there is a need for a microfluidic chip imaging system with good performance and portability.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a portable micro-fluidic chip imaging system. As shown in fig. 1, the imaging system ensures the consistency of the height, angle and illumination condition of each shooting when the reaction result in the microfluidic chip is obtained by the mobile phone, has low requirement on the external environment, greatly improves the accuracy of the reading result, has good repeatability and satisfactory effect, is convenient to carry, and is very suitable for imaging the microfluidic chip.

The invention is realized by the following technical scheme:

the invention discloses a portable microfluidic chip imaging system which comprises an illuminating device, an LED bulb arranged on the illuminating device and a microfluidic chip corresponding to the bottom of the illuminating device, wherein a window is formed in the upper part of the illuminating device, an opening is formed in the lower part of the illuminating device, the LED bulb is arranged along the periphery of the window, and the diameter of the microfluidic chip correspondingly arranged on the opening in the lower part is equal to the inner diameter of the opening.

As a further improvement, the LED bulb is arranged downwards, and the upper surface of the lighting device is a flat plane.

As a further improvement, the LED bulbs are arranged along the periphery of the window for 1-4 circles, the distance between each LED bulb in the same circle is equal, the distance between each LED bulb in the same circle and the circular window is equal, and the size and the power of all the LED bulbs are equal.

As a further improvement, the microfluidic chip comprises a sample inlet area, a linear channel and a detection area, wherein the LED bulb corresponds to the detection area.

As a further improvement, the number of the LED bulbs in the lighting device of the present invention is 40, wherein 16 bulbs are arranged in the inner ring, 24 bulbs are arranged in the outer ring, 1 group of every 2 bulbs in the inner ring and every 3 bulbs in the outer ring correspond to each other, 1 group of LED bulbs corresponds to 1 detection area in the circular microfluidic chip, and 8 groups of LED bulbs exactly correspond to 8 detection areas in the circular microfluidic chip.

The invention also discloses application of the portable micro-fluidic chip imaging system in quantitative analysis of any substance. When imaging is carried out, the microfluidic chip is just horizontally embedded into the circular opening at the lower part of the lighting device, then the equipment bearing the lens is tightly attached to the upper surface of the lighting device, the lens is arranged above the window of the lighting device, the microfluidic chip, the lighting device and the equipment bearing the lens form a dark room which cannot be entered by external light, then the power supply of the lighting device is switched on, the LED bulb emits uniform light downwards, and finally the equipment bearing the lens captures the image of the microfluidic chip.

As a further improvement, the device for bearing the lens is a mobile phone.

The invention has the following technical effects:

1) during imaging, the micro-fluidic chip, the lighting device and the mobile phone form a darkroom which can not be entered by external light, and the power of the LED bulb and the relative position of the LED bulb and the detection area of the micro-fluidic chip are fixed, so that the illumination conditions of each imaging are consistent. During imaging, the lens of the mobile phone captures images through the circular window above the lighting device, so that the height and the angle of each imaging are consistent. Meanwhile, the mobile phone can be supported by the lighting device during imaging, so that the problem of shaking during shooting is solved. In conclusion, the imaging system ensures that the height, angle and illumination condition of each shooting are consistent when the reaction result in the microfluidic chip is obtained through the mobile phone, the requirement on the external environment is low, the accuracy of the reading result is greatly improved, the repeatability is good, and the effect is satisfactory.

2) Compared with the scanner for imaging, the imaging system is small in size and convenient to carry, the imaging system cannot be in direct contact with a reaction area in the microfluidic chip, the interior of the imaging system cannot be polluted by chemical reagents, the cleaning trouble is solved, the potential safety hazard is reduced, and the equipment does not have obvious vibration as the scanner during imaging, so that the imaging quality is higher. In addition, almost people have one or more mobile phones at present, and the cost of the device is greatly reduced from the perspective of hardware devices.

Drawings

Fig. 1 is a portable microfluidic chip imaging system provided by the present invention.

In the figure, 1 is an LED bulb, 2 is an illuminating device, 3 is a microfluidic chip, 4 is a sample injection area, 5 is a linear channel, and 6 is a detection area.

The lighting device 2 is a completely opaque dark box environment, and its upper surface is shown as transparent in fig. 1 for the purpose of showing its internal structure.

Detailed Description

The invention provides a portable micro-fluidic chip imaging system, which consists of a micro-fluidic chip 3 and an illuminating device 2, and takes a micro-fluidic channel with a snowflake shape in the center of the circular micro-fluidic chip 3 as an example, the system comprises 1 sample injection area 4, 8 linear channels 5, 8 detection areas 6 and the like.

Fig. 1 is a portable microfluidic chip imaging system provided by the invention, and the specific implementation manner is as follows:

(1) the diameter of circular micro-fluidic chip 3 is 50mm, thickness is 5mm, its center has the micro-fluidic channel of snowflake shape, 3 parts such as injection zone 4, linear type channel 5 and detection zone 6 have been included, micro-fluidic channel's degree of depth is 1mm, wherein, the diameter of 1 injection zone 4 in center is 10mm, the diameter of 8 detection zones 6 on every side is 8mm, the centre of a circle of central injection zone 4 and detection zone 6 on every side is 16mm apart, the width of linear type channel 5 is 2mm, the micro-fluidic channel is filled with the cellulose powder.

(2) The upper portion of lighting device 2 is opened has 1 circular window that the diameter is 2cm, 2 rings of LED bulbs 1 that face down are evenly installed along circular window at the top (ceiling) of lighting device 2 inside, wherein the inner circle has 16 LED bulbs 1, there are 24 LED bulbs 1 in the outer lane, every 2 LED bulbs 1 of inner circle are 1 group rather than every 3 LED bulbs 1 that the outer lane corresponds, every 1 LED bulb 1 of group corresponds with 1 detection zone 6 in the circular micro-fluidic chip 3, 8 LED bulbs 1 of group just correspond 8 detection zones 6 in the circular micro-fluidic chip 3. All the LED bulbs 1 are equal in size and power, the distance between each LED bulb 1 in the same circle is equal, the distance between each LED bulb 1 in the same circle and the circular window is also equal, and the LED bulbs 1 are powered through the external wires in the graph 1. The lighting device 2 is 8cm high and opens downwards with an internal diameter of 5 cm.

(3) Adding various reagents required by reaction into a detection area 6 and a sample injection area 4 in a micro-fluidic chip 3 by using a pipette, after the color reaction is finished, placing the micro-fluidic chip 3 on a plane, placing an illuminating device 2 above the chip, enabling the chip to be just horizontally embedded into a circular opening at the lower part of the illuminating device 2, then tightly attaching a mobile phone to the upper surface of the illuminating device 2, placing a lens of the mobile phone above a circular window of the illuminating device 2, enabling the micro-fluidic chip 3, the illuminating device 2 and the mobile phone to jointly form a dark room in which external light cannot enter, then switching on a power supply of the illuminating device 2 to enable the LED bulb 1 to downwards emit uniform light, and finally capturing an image of the micro-fluidic chip 3 by using the mobile phone.

It is obvious that the above is not a limitation of the embodiments, and it is obvious for a person skilled in the art that various modifications can be made on the above description, and these modified embodiments are still within the protection scope of the present invention.

Claims

1. A portable microfluidic chip imaging system is characterized by comprising an illuminating device (2), LED bulbs (1) arranged on the illuminating device (2) and microfluidic chips (3) corresponding to the bottoms of the illuminating device (2), wherein the upper part of the illuminating device (2) is provided with a window, the lower part of the illuminating device is provided with an opening, the LED bulbs (1) are arranged along the periphery of the window, the diameter of the microfluidic chip (3) correspondingly arranged on the lower opening is equal to the inner diameter of the opening, the LED bulbs (1) are arranged downwards, the surface of the upper part of the illuminating device (2) is a flat plane, the LED bulbs (1) are arranged in 1-4 circles along the periphery of the window, the distance between each LED bulb (1) in the same circle is equal, the distance between each LED bulb (1) in the same circle and the circular window is equal, and the size and the power of all the LED bulbs (1) are equal, micro-fluidic chip (3) including advancing appearance district (4), linear type passageway (5) and detection zone (6), LED bulb (1) corresponding with detection zone (6), inside LED bulb (1) of lighting device (2) is total 40, wherein the inner circle has 16, the outer lane has 24, every 2 bulbs of inner circle are 1 group rather than every 3 bulbs that the outer lane corresponds, every 1 LED bulb (1) of group corresponds with 1 detection zone (6) in the circular micro-fluidic chip (3), 8 LED bulbs of group (1) correspond 8 detection zones (6) in the circular micro-fluidic chip (3) just.

2. A method for applying the portable microfluidic chip imaging system according to claim 1 to any substance quantitative analysis, wherein during imaging, the microfluidic chip (3) is just horizontally embedded into the circular opening at the lower part of the illumination device (2), then the device bearing the lens is tightly attached to the upper surface of the illumination device (2), the lens is placed above the window of the illumination device (2), so that the microfluidic chip (3), the illumination device (2) and the device bearing the lens form a dark room which can not be entered by external light, then the power supply of the illumination device (2) is switched on to make the LED bulb (1) downwards emit uniform light, and finally the device bearing the lens is used for capturing the image of the microfluidic chip (3).

3. The method of claim 2, wherein the lens-bearing device is a cellular phone.