CN107581163B - Method for feeding cryptopterus gracilis by using tetranychus cinnabarinus - Google Patents
Method for feeding cryptopterus gracilis by using tetranychus cinnabarinus Download PDFInfo
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- CN107581163B CN107581163B CN201711072138.2A CN201711072138A CN107581163B CN 107581163 B CN107581163 B CN 107581163B CN 201711072138 A CN201711072138 A CN 201711072138A CN 107581163 B CN107581163 B CN 107581163B
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Abstract
The invention belongs to the technical field of indoor propagation and breeding of natural enemy insects, and particularly relates to a method for breeding cryptopteris luteoloides by utilizing tetranychus cinnabarinus. Feeding tetranychus cinnabarinus as prey under the conditions that the temperature is 25 +/-2 ℃, the relative humidity is 70-90% and the light-dark ratio is 16h:8h, and feeding tetranychus cinnabarinus to cryptopteris luteolor. The cryptopteris crocus littoralis with the advantages of strong continuous subculture capability, difficult degeneration of seed properties (preying capability on preys and the like) and the like is bred by specific technical measures such as preparation of host plants, transfer and breeding of tetranychus cinnabarinus, breeding of cryptopteris crocus littoralis and the like, and has very important significance for providing stable laboratory insect sources and carrying out biological research and application technical research of the natural enemy insects.
Description
Technical Field
The invention belongs to the technical field of indoor propagation and breeding of natural enemy insects, and particularly relates to a method for breeding cryptopteris luteoloides by utilizing tetranychus cinnabarinus.
Background
Tetranychus cinnabarinus belongs to the families of Arachnida, Eudermales and Tetranychidae, is an important pest mite of vegetables of the families of Solanaceae, Leguminosae and Cucurbitaceae and is also an important pest mite of a plurality of flowers. The application of chemical pesticides is a main means for controlling the harm of tetranychus cinnabarinus at present, but the problems of drug resistance, residue and the like of the tetranychus cinnabarinus are increasingly prominent along with the application of a large amount of pesticides.
Acarina luteo-acarus cryptopteris (A. luteo)Oligota flavicornis) Belongs to the families of coleoptera and cryptopteridae, and is an important local predatory natural enemy insect species on various crops such as oranges, roses, eggplants, tomatoes, corns, cassava and the like in southern China. The larvae and adults of the mites can prey on tetranychus cinnabarinus, tetranychus urticae koch and the like, are important natural enemies of tetranychus urticae koch, and play an important role in preventing and controlling tetranychus urticae koch. A method for artificially breeding a large amount of cryptopterus flavipes by using Tyrophagus putrescentiae, Glycyphylla teres and the like as alternative feeds is disclosed in China (patent number: CN 10773101B). However, basic research on the natural enemy insect is still insufficient, and in order to deeply develop research on biology, ethology, physiology and the like, the cryptoptera xylophaga which takes natural prey as feed needs to be provided as an experimental insect source all the year around so as to obtain research data according with the natural occurrence rule. The invention provides a method for feeding cryptopterus flavus by using natural hobby preys (tetranychus cinnabarinus) annually.
Disclosure of Invention
The invention aims to provide a method for feeding cryptopteris luteolor by using natural prey Tetranychus cinnabarinus annually.
In order to realize the purpose, the following technical scheme is adopted:
a method for feeding Tetranychus telarius with Tetranychus cinnabarinus comprises the following specific steps:
(1) preparation of host plants
Cutting the sponge into a square shape, paving the square shape at the bottom of the tetranychus cinnabarinus breeding box, and adding water into the tetranychus cinnabarinus breeding box to enable the tetranychus cinnabarinus breeding box to permeate into the sponge naturally; picking the planted host plant leaves, placing the leaves on the sponge with the back face upward, and wrapping the top ends of the main veins with wet cotton to keep the leaves fresh.
The tetranychus cinnabarinus breeding box is a square plastic box with the length of 17cm, the width of 10cm and the height of 7cm, and a plurality of round holes with the diameter of 0.5cm are punched on the box for ventilation.
(2) Tetranychus cinnabarinus transfer
Putting host plant leaves carrying tetranychus cinnabarinus as an inoculation carrier on fresh host plant leaves, and after 2d, removing the leaves of the inoculation carrier of tetranychus cinnabarinus after 100-200 adult mites settle on the fresh leaves, so that the tetranychus cinnabarinus naturally settles and breeds on the fresh host plant leaves;
(3) breeding of tetranychus cinnabarinus
Placing the inoculated host plant leaves in the step (2) into an artificial incubator with the temperature of 25 +/-2 ℃, the relative humidity of 70-90% and the light-dark ratio of 16h:8h for culture; and timely injecting water into the tetranychus cinnabarinus feeding box to keep the humidity, wherein the water level line is not too high so as to prevent the back of the leaf from being wetted and the tetranychus cinnabarinus from dying.
(4) Breeding of mite-eating cryptopterus volvatus imagoes
Tetranychus cinnabarinus is a main pest mite which is harmful to vegetables and fruit trees, and the mite-eating cryptopteris pteronymus is used as a natural enemy, can prey on farmland pest mites such as Tetranychus cinnabarinus and the like, and can collect insect sources from leaves of vegetables and fruit trees which are harmful to mites.
Putting the collected adults and the host plant leaves with tetranychus cinnabarinus into the cryptopterus flavipes feeding containers together, wherein 10 cryptopterus flavipes adults in each feeding container are placed; taking out the leaves (the eggs of the cryptopteris luteinii) every 4 days, picking out the eggs with a writing brush to fresh leaves with tetranychus cinnabarinus, wherein the ratio of the egg picking amount to prey is 1: 300-400; and then a new fresh leaf with tetranychus cinnabarinus growing thereon is selected and placed in the container for feeding the cryptopterus xanthipes to feed. The opening of the container needs to be perforated with a plurality of round holes with the diameter of 1.0cm for ventilation, and then the round holes are stuck by gauze to prevent adults from escaping.
The container for raising the adult cyenophagus xanthina larvae is a transparent circular plastic tank with the height of 20cm and the diameter of 10cm, a plurality of circular holes with the diameter of 1.0cm are punched on the tank for ventilation, and the circular holes are stuck by gauze to prevent the adult cyenophagus xanthina larvae from escaping.
(5) Pupation and eclosion
After 7-10 days, picking out the larvae to fresh leaves of host plants with preys (tetranychus cinnabarinus) when the larvae grow to 3 years old, putting the leaves into a container for feeding the cryptopterus xanthipes, placing a piece of wet filter paper at the bottom of the container, enabling the 3 years old larvae to climb down to the filter paper for pupation, adding water to the filter paper in time during the period, controlling the humidity to be 70-90%, and controlling the picking amount of the larvae in each container to be 20-30. After eclosion, picking out the adult cryptopterus crocus flavus to be bred in a cryptopterus crocus flavus breeding container.
The invention has the advantages that: the host plant adopted by the invention has no special requirements, is common and easy to obtain, and has relatively low cost. The cryptopteris luteolor bred by the method has strong continuous subculture capability, the seed properties (predation capability to prey on prey and the like) are not easy to degrade, and enough experimental insect sources can be stably provided, so that the requirement of further developing various entomology researches can be met.
Drawings
FIG. 1: is a block diagram of the steps of the present invention.
Detailed Description
The process of the present invention is described in further detail below by way of examples, but it should be understood that the invention is not limited thereto.
Example 1 selecting soybean leaves as host plants for breeding tetranychus cinnabarinus, tetranychus cinnabarinus as prey for feeding cryptopterus xanthineus,
(1) preparation of host plants
Cutting the sponge into a square shape, paving the square shape at the bottom of the tetranychus cinnabarinus breeding box, and adding water into the tetranychus cinnabarinus breeding box to enable the tetranychus cinnabarinus breeding box to permeate into the sponge naturally; picking the planted host plant leaves, placing the leaves on the sponge with the back face upward, and wrapping the top ends of the main veins with wet cotton to keep the leaves fresh.
The tetranychus cinnabarinus breeding box is a square plastic box with the length of 17cm, the width of 10cm and the height of 7cm, and a plurality of round holes with the diameter of 0.5cm are punched on the box for ventilation.
(2) Tetranychus cinnabarinus transfer
Putting host plant leaves carrying tetranychus cinnabarinus as an inoculation carrier on fresh host plant leaves, and after 2 days, removing the leaves of the inoculation carrier of tetranychus cinnabarinus after 200 adult mites settle on the fresh leaves, so that the tetranychus cinnabarinus naturally settles and breeds on the fresh host plant leaves;
(3) breeding of tetranychus cinnabarinus
Placing the inoculated host plant leaves in the step (2) into an artificial incubator with the temperature of 25 +/-2 ℃, the relative humidity of 80 percent and the light-dark ratio of 16h to 8h for culture; and timely injecting water into the tetranychus cinnabarinus feeding box to keep the humidity, wherein the water level line is not too high so as to prevent the back of the leaf from being wetted and the tetranychus cinnabarinus from dying.
(4) Breeding of mite-eating cryptopterus volvatus imagoes
Tetranychus cinnabarinus is a main pest mite which is harmful to vegetables and fruit trees, and the mite-eating cryptopteris pteronymus is used as a natural enemy, can prey on farmland pest mites such as Tetranychus cinnabarinus and the like, and can collect insect sources from leaves of vegetables and fruit trees which are harmful to mites.
Putting the collected adults and the host plant leaves with tetranychus cinnabarinus into the cryptopterus flavipes feeding containers together, wherein 10 cryptopterus flavipes adults in each feeding container are placed; taking out leaves (containing eggs of the yellow horned acarus fuscipes) at 4d per cadmium, picking out the eggs with a writing brush to fresh leaves with tetranychus cinnabarinus, wherein the ratio of the egg picking amount to prey is 1: 400; and then a new fresh leaf with tetranychus cinnabarinus growing thereon is selected and placed in the container for feeding the cryptopterus xanthipes to feed. The opening of the container needs to be perforated with round holes with the diameter of 1.0cm for ventilation, and then the round holes are stuck by gauze to prevent adults from escaping.
The container for raising the adult cyenophagus xanthina larvae is a transparent circular plastic tank with the height of 20cm and the diameter of 10cm, a plurality of circular holes with the diameter of 1.0cm are punched on the tank for ventilation, and the circular holes are stuck by gauze to prevent the adult cyenophagus xanthina larvae from escaping.
(5) Pupation and eclosion
After 7 days, picking out the 3 rd larvae to fresh leaves of host plants with preys (tetranychus cinnabarinus), putting the leaves into a container for rearing the cryptopterus xanthipes, placing a piece of wet filter paper at the bottom of the container, enabling the 3 rd larvae to climb down to the filter paper for pupation, adding water to the filter paper in time during the period, controlling the moisture retention degree to be 80%, and controlling the picking amount of the larvae in each container to be between 20 and 30. After eclosion, picking out the adult cryptopterus crocus flavus to be bred in a cryptopterus crocus flavus breeding container.
The feeding results are shown in table 1:
TABLE 1 results of feeding of Amyda flavipes Cryptoptera
The cryptopteris luteolor bred by the method has strong continuous subculture capability, the seed properties (predation capability to prey on prey and the like) are not easy to degrade, and enough experimental insect sources can be stably provided, so that the requirement of further developing various entomology researches can be met.
Specifically, the following description is provided: the invention has no special requirements for the selection of host plants, but the soybean leaves are more ideal from the aspects of cost and practicability. Therefore, the present invention is described by taking soybean leaves as an example.
Claims (1)
1. A method for feeding Tetranychus telarius with Tetranychus cinnabarinus is characterized by comprising the following steps: the method comprises the following steps:
(1) preparation of host plants
Cutting the sponge into a square shape, paving the square shape at the bottom of the tetranychus cinnabarinus breeding box, and adding water into the tetranychus cinnabarinus breeding box to enable the tetranychus cinnabarinus breeding box to permeate into the sponge naturally; picking planted host plant leaves, placing the leaves on a sponge with the back face upward, and wrapping main veins of the leaves with wet cotton to keep the leaves fresh;
(2) tetranychus cinnabarinus transfer
Putting host plant leaves carrying tetranychus cinnabarinus as an inoculation carrier on fresh host plant leaves, and after 2d, removing the leaves of the inoculation carrier of tetranychus cinnabarinus after 100-200 adult mites settle on the fresh leaves, so that the tetranychus cinnabarinus naturally settles and breeds on the fresh host plant leaves;
(3) breeding of tetranychus cinnabarinus
Placing the tetranychus cinnabarinus breeding box inoculated in the step (2) into an artificial incubator at the temperature of 25 +/-2 ℃, the relative humidity of 70-90% and the light-dark ratio of 16h to 8h for culturing; injecting water into the tetranychus cinnabarinus breeding box in time to keep the humidity, wherein the water level line is not too high to avoid wetting the back of the leaves to cause the death of the tetranychus cinnabarinus;
(4) breeding of natural enemy paederus
Selecting healthy and active individuals from the wild collected adult cryptopterus flavomarginatus as an insect source for indoor propagation; putting the collected adults into a cryptopterus crocus erosus feeding container for mating and oviposition, wherein host plant leaves with tetranychus cinnabarinus are put into the container for feeding and moving, and 10 cryptopterus crocus adults in each feeding container; taking out host plant leaves every 4 days, namely taking eggs of the cryptopteris luteolor on the leaves, picking the cryptopteris luteolor eggs on the leaves to the fresh host plant leaves with tetranychus cinnabarinus, and feeding the host plant leaves with the eggs in a ratio of 1: 300-400; after 7-10 days, putting the host plant leaves with the cryptopteris larvae into a cryptopteris flavedo acarus feeding container, putting a piece of wet filter paper at the bottom of the container, enabling the 3-year larvae to climb down to the filter paper to pupate, dripping water on the filter paper in time during the period, controlling the humidity to be 70-90%, and controlling the picking amount of the larvae in each container to be 20-30; when eclosion is adult, one part of the adult is used as an insect source to continue propagation, and the rest of the adult is used as test insects;
the tetranychus cinnabarinus breeding box in the step (1) is a square plastic box with the length of 17cm, the width of 10cm and the height of 7cm, and a plurality of round holes with the diameter of 0.5cm are punched for ventilation;
and (4) covering a transparent circular plastic tank with the height of 20cm and the diameter of 10cm with a plurality of circular holes with the diameter of 1.0cm for ventilation, and sticking the circular holes with gauze to prevent the adult cyenophagus xanthina mites from escaping.
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Patent Citations (7)
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| CN101331869A (en) * | 2008-08-07 | 2008-12-31 | 北京市农林科学院 | Chile phytoseiulus persimilis propagating method |
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