CN107581163A - It is a kind of that the method for eating mite rove beetle in yellow angle is raised using Tetranychus cinnabarinus - Google Patents
It is a kind of that the method for eating mite rove beetle in yellow angle is raised using Tetranychus cinnabarinus Download PDFInfo
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- CN107581163A CN107581163A CN201711072138.2A CN201711072138A CN107581163A CN 107581163 A CN107581163 A CN 107581163A CN 201711072138 A CN201711072138 A CN 201711072138A CN 107581163 A CN107581163 A CN 107581163A
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- tetranychus cinnabarinus
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- rove beetle
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Abstract
The invention belongs to natural enemy insect indoor propagation and breeding technology field, and in particular to a kind of that the method for eating mite rove beetle in yellow angle is raised using Tetranychus cinnabarinus.Temperature be 25 DEG C ± 2 DEG C, relative humidity 70% 90%, Light To Dark Ratio 16h:In the environment of 8h, raising Tetranychus cinnabarinus raises yellow angle using Tetranychus cinnabarinus and eats mite rove beetle as prey.Raised out by the particular technique measure such as the preparation of host plant, the switching of Tetranychus cinnabarinus and raising, the raising of yellow angle food mite rove beetle with continuous subculture ability is strong, kind property(To prey prey ability etc.)The yellow angle food mite rove beetle for the advantages that being not easy to degenerate, for providing stable laboratory worm sources, the biological study and application technical research tool of carrying out the natural enemy insect in a deep going way are of great significance.
Description
Technical field
The invention belongs to natural enemy insect indoor propagation and breeding technology field, and in particular to one kind is raised using Tetranychus cinnabarinus
The method of yellow angle food mite rove beetle.
Background technology
Tetranychus cinnabarinus belongs to Arachnoidea, Acariformes, Tetranychidae, is the important evil mite of the vegetables such as Solanaceae, pulse family and Curcurbitaceae,
And the important evil mite of many flowers.It is the Main Means of current control cowpea seedlings using chemical pesticide, it is still, adjoint
A large amount of administrations of agricultural chemicals, become increasingly conspicuous the problems such as the evil mite resistance to the action of a drug, residual.
Yellow angle food mite rove beetle (Oligota flavicornis) belong to coleoptera, Staphylinidae, it is southern area of China
Important native country predatory natural enemy insect species in the various crops such as citrus, rose, eggplant, tomato, corn and cassava.Its children
Worm, adult can prey on Tetranychus cinnabarinus, Tetranychus urticae etc., be the Important Natural Enemy of spider mite kind, play and focus in terms of tetranychid is prevented and treated
The effect wanted.The country has been disclosed for one kind and substitutes the artificial mass rearing Huang angle food mite of feed using tyrophagus putrescentiae, hesperidium mite etc.
The method of rove beetle(The patent No.:CN 10773101 B).But the basic research on the natural enemy insect is also short of very much, in order to
The research of its biology, behaviouristics, physiology etc. is carried out in a deep going way, it is necessary to provide in the anniversary hidden as the food mite of food using natural prey
Wing worm is as experiment worm sources, to obtain the data for meeting its natural occurrence patterns.The invention provides one kind to utilize day
So hobby prey(Tetranychus cinnabarinus)The method for raising yellow angle food mite rove beetle the anniversary.
The content of the invention
The yellow angle food hidden wing of mite of natural hobby prey Tetranychus cinnabarinus anniversary raising is utilized it is an object of the invention to provide a kind of
The method of worm.
To achieve the above object, using following technical scheme:
A kind of method that yellow angle food mite rove beetle is raised using Tetranychus cinnabarinus is concretely comprised the following steps:
(1)The preparation of host plant
Sponge cutting squarely is laid in the bottom of Tetranychus cinnabarinus raising box, adds water toward Tetranychus cinnabarinus raising box, makes its nature
Infiltration is in sponge;Pluck plantation host plant blades, be placed on sponge, the back side upward, and with moisten cotton
Its main lobe arteries and veins top is wrapped up, keeps the freshness of blade.
Described Tetranychus cinnabarinus raising box is long 17cm, wide 10cm, high 7cm square plastic magazine, covers that to beat several straight
Footpath is that 0.5cm circular hole is breathed freely.
(2)The switching of Tetranychus cinnabarinus
It is placed on the host plant blades for carrying Tetranychus cinnabarinus as inoculation carrier in fresh host's blade, after 2d, treats piece
100~200 are settled down on fresh blade into after mite, the inoculation carrier blade of Tetranychus cinnabarinus is removed, makes Tetranychus cinnabarinus fresh
Naturally breeding is settled down in host plant blades;
(3)The raising of Tetranychus cinnabarinus
By step(2)It is 25 DEG C ± 2 DEG C, relative humidity 70%~90%, brightness that the middle host plant blades being inoculated with, which are placed in temperature,
Compare 16h:Cultivated in 8h artificial incubator;And box water filling is raised to Tetranychus cinnabarinus in time, humidity is kept, but waterline should not be too
Height causes Tetranychus cinnabarinus dead in order to avoid adhesional wetting vacuum side of blade.
(4)Eat the raising of mite rove beetle adult
Tetranychus cinnabarinus is the main evil mite of a kind of vegetables of causing harm, fruit tree, and food mite rove beetle can prey on Zhu as a kind of natural enemy
The farmland such as sand tetranychid evil mite, worm sources can be collected from the vegetables done harm to by mite, on fruit tree leaves.
The adult collected is together put into yellow angle food mite rove beetle with the host plant blades with Tetranychus cinnabarinus to raise
In container, each yellow angle food mite rove beetle adult 10 raised in container;Per 4d, blade is taken out(I.e. containing Huang on blade
Eat the ovum of mite rove beetle in angle), choose ovum to the fresh blade with Tetranychus cinnabarinus with writing brush, the ratio chosen into amount and prey of ovum
Example is with 1:300~400;And select a fresh blade with Tetranychus cinnabarinus else again and be placed on yellow angle food mite rove beetle raising appearance
In device, for eating mite rove beetle feeding.The circular hole that the tank mouth of container needs to beat some diameter 1.0cm is breathed freely, then is clung with gauze
Circular hole, to prevent adult from escaping.
Described yellow angle food mite rove beetle imago breeding container is high 20cm, diameter 10cm transparent circular plastic tank, is covered
The upper circular hole for beating several a diameter of 1.0cm is breathed freely, and circular hole is clung with grenadine prevents yellow angle food mite rove beetle adult from escaping.
(5)Pupate and sprout wings
After 7 ~ 10d, choose when its development is 3 instar larvae to fresh and carry prey(Tetranychus cinnabarinus)Host plant blades
On, the blade is put into yellow angle and eaten in mite rove beetle raising container, and the filter paper of a moistening, 3 ages children are placed in container bottom
Worm, which can be down climbed on filter paper, to pupate, in the meantime will in time toward adding water on filter paper, humid control 70%~90%, Mei Gerong
The amount of choosing of larva should be controlled between 20~30 in device.After emergence, choose yellow angle food mite rove beetle adult and eaten in yellow angle
Raised in mite rove beetle raising container.
The advantage of the invention is that:The host plant that the present invention uses, does not require particularly, common to be easy to get, cost phase
To relatively low.Strong, the kind property using the yellow angle food continuous subculture ability of mite rove beetle of this method raising(To prey prey ability etc.)No
Easily degenerate, and can stablize and enough experiment worm sources are provided, disclosure satisfy that the needs for carrying out every entomology research in a deep going way.
Brief description of the drawings
Fig. 1:For step block diagram of the present invention.
Embodiment
The inventive method is described in further detail by the following examples, but it is understood that the present invention is not by this
A little contents are limited.
Embodiment 1 is eaten from host plant of the Folium Glycines as breeding Tetranychus cinnabarinus using Tetranychus cinnabarinus as yellow angle is raised
The prey of mite rove beetle,
(1)The preparation of host plant
Sponge cutting squarely is laid in the bottom of Tetranychus cinnabarinus raising box, adds water toward Tetranychus cinnabarinus raising box, makes its nature
Infiltration is in sponge;Pluck plantation host plant blades, be placed on sponge, the back side upward, and with moisten cotton
Its main lobe arteries and veins top is wrapped up, keeps the freshness of blade.
Described Tetranychus cinnabarinus raising box is long 17cm, wide 10cm, high 7cm square plastic magazine, covers that to beat several straight
Footpath is that 0.5cm circular hole is breathed freely.
(2)The switching of Tetranychus cinnabarinus
It is placed on the host plant blades for carrying Tetranychus cinnabarinus as inoculation carrier in fresh host's blade, after 2 days, treats piece
200 are settled down on fresh blade into after mite, the inoculation carrier blade of Tetranychus cinnabarinus is removed, makes Tetranychus cinnabarinus in fresh host plant
Naturally breeding is settled down on blade;
(3)The raising of Tetranychus cinnabarinus
By step(2)The middle host plant blades being inoculated be placed in temperature for 25 DEG C ± 2 DEG C, relative humidity 80%, Light To Dark Ratio 16h:
Cultivated in 8h artificial incubator;And in time give Tetranychus cinnabarinus raising box water filling, keep humidity, but waterline should not it is too high in order to avoid
Vacuum side of blade is bedewed, causes Tetranychus cinnabarinus dead.
(4)Eat the raising of mite rove beetle adult
Tetranychus cinnabarinus is the main evil mite of a kind of vegetables of causing harm, fruit tree, and food mite rove beetle can prey on Zhu as a kind of natural enemy
The farmland such as sand tetranychid evil mite, worm sources can be collected from the vegetables done harm to by mite, on fruit tree leaves.
The adult collected is together put into yellow angle food mite rove beetle with the host plant blades with Tetranychus cinnabarinus to raise
In container, each yellow angle food mite rove beetle adult 10 raised in container;Blade is taken out per cadmium 4d(I.e. containing Huang on blade
Eat the ovum of mite rove beetle in angle), choose ovum to the fresh blade with Tetranychus cinnabarinus with writing brush, the ratio chosen into amount and prey of ovum
Example is with 1: 400;And select a fresh blade with Tetranychus cinnabarinus else again and be placed on yellow angle food mite rove beetle raising container
In, for eating mite rove beetle feeding.The circular hole that the tank mouth of container needs to beat some diameters 1.0cm is breathed freely, then clings circle with gauze
Hole, to prevent adult from escaping.
Described yellow angle food mite rove beetle imago breeding container is high 20cm, diameter 10cm transparent circular plastic tank, is covered
The upper circular hole for beating several a diameter of 1.0cm is breathed freely, and circular hole is clung with grenadine prevents yellow angle food mite rove beetle adult from escaping.
(5)Pupate and sprout wings
After 7d, choose when its development is 3 instar larvae to fresh and carry prey(Tetranychus cinnabarinus)Host plant blades on, will
The blade is put into yellow angle food mite rove beetle raising container, and places the filter paper of a moistening in container bottom, and 3 instar larvaes can be past
Under climb on filter paper and pupate, in the meantime will be in time toward on filter paper plus water, moisturizing degree control the larva in 80%, each container
The amount of choosing is controlled between 20~30.After emergence, choose yellow angle food mite rove beetle adult in the food mite rove beetle raising of yellow angle
Raised in container.
Raising the results are shown in Table 1:
Eat the raising result of mite rove beetle in 1 yellow angle of table
Strong, the kind property using the yellow angle food continuous subculture ability of mite rove beetle of this method raising(To prey prey ability etc.)It is not easy to move back
Change, and can stablize and enough experiment worm sources are provided, disclosure satisfy that the needs for carrying out every entomology research in a deep going way.
Special instruction:Selection of the present invention to host plant does not specially require, but considers from cost and point of view of practicability,
It is ideal from soya bean blade.So the present invention is only explained by taking Folium Glycines as an example.
Claims (3)
1. a kind of raise the method for eating mite rove beetle in yellow angle using Tetranychus cinnabarinus, it is characterised in that:Comprise the following steps:
(1)The preparation of host plant
Sponge cutting squarely is laid in the bottom of Tetranychus cinnabarinus raising box, adds water toward Tetranychus cinnabarinus raising box, makes its nature
Infiltration is in sponge;Pluck plantation host plant blades, be placed on sponge, the back side upward, and with moisten cotton
Its main lobe arteries and veins is wrapped up, keeps the freshness of blade;
(2)The switching of Tetranychus cinnabarinus
It is placed on the host plant blades for carrying Tetranychus cinnabarinus as inoculation carrier in fresh host's blade, after 2d, treats piece
100~200 are settled down on fresh blade into after mite, the inoculation carrier blade of Tetranychus cinnabarinus is removed, makes Tetranychus cinnabarinus fresh
Naturally breeding is settled down in host plant blades;
(3)The raising of Tetranychus cinnabarinus
By step(2)It is 25 DEG C ± 2 DEG C, relative humidity 70%~90%, light that the middle Tetranychus cinnabarinus raising box being inoculated with, which is placed in temperature,
Secretly compare 16h:Cultivated in 8h artificial incubator;And box water filling is raised to keep humidity to Tetranychus cinnabarinus in time, but waterline is not
It is preferably too high in order to avoid adhesional wetting vacuum side of blade, causes Tetranychus cinnabarinus dead;
(4)The raising of natural enemy rove beetle
The individual of bouncing is selected as the worm sources of indoor propagation using in the yellow angle food mite rove beetle adult of field acquisition;It will adopt
The adult of collection is put into mate and oviposit in yellow angle food mite rove beetle raising container, and should be put into the host with Tetranychus cinnabarinus in container plants
Thing blade supplies its feeding and activity, each yellow angle food mite rove beetle adult 10 raised in container;Every 4d, take out host and plant
Thing blade, the ovum on blade i.e. containing yellow angle food mite rove beetle, the rove beetle ovum chosen on the blade carry cinnabar leaf to fresh
Raised in the host plant blades of mite, ovum is chosen into amount and the ratio of prey with 1:300~400;Rove beetle will be carried after 7 ~ 10d
The host plant blades of larva are put into yellow angle food mite rove beetle raising container, and place the filter of a moistening in container bottom
Paper, 3 instar larvaes, which can be climbed to down on filter paper, pupates, in the meantime will in time toward dripping on filter paper, humid control 70%~90%,
The amount of choosing of larva is controlled between 20~30 in each container;When turning into adult, a part continues numerous as worm sources
Grow, remaining adult is used as experiment with worm.
A kind of 2. method that yellow angle food mite rove beetle is raised using Tetranychus cinnabarinus according to claim 1, it is characterised in that:
Step(1)Described Tetranychus cinnabarinus raising box is long 17cm, wide 10cm, high 7cm square plastic magazine, covers and beats several diameters
Breathed freely for 0.5cm circular hole.
A kind of 3. method that yellow angle food mite rove beetle is raised using Tetranychus cinnabarinus according to claim 1, it is characterised in that:
Step(4)Described yellow angle food mite rove beetle imago breeding container is high 20cm, diameter 10cm transparent circular plastic tank, is covered
The circular hole for beating several a diameter of 1.0cm is breathed freely, and circular hole is clung with grenadine prevents yellow angle food mite rove beetle adult from escaping.
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Cited By (1)
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CN108713531A (en) * | 2018-05-25 | 2018-10-30 | 福建省农业科学院植物保护研究所 | A kind of method that Highly Effective collects natural enemy food mite Staphylinus insect pupa |
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CN104222019A (en) * | 2013-06-21 | 2014-12-24 | 中国农业科学院植物保护研究所 | Method for artificial mass production of spider mite natural enemies |
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Patent Citations (7)
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CN101331869A (en) * | 2008-08-07 | 2008-12-31 | 北京市农林科学院 | Chile phytoseiulus persimilis propagating method |
CN101406167A (en) * | 2008-09-25 | 2009-04-15 | 福建农林大学 | Method for considerably feeding and using ascidae |
CN101773101A (en) * | 2010-03-03 | 2010-07-14 | 中国农业科学院植物保护研究所 | Method for artificially and massively feeding natural enemy staphylinid |
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CN108713531A (en) * | 2018-05-25 | 2018-10-30 | 福建省农业科学院植物保护研究所 | A kind of method that Highly Effective collects natural enemy food mite Staphylinus insect pupa |
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