CN107540100B - OUR-based activated sludge bulking early warning analysis system and using method thereof - Google Patents

OUR-based activated sludge bulking early warning analysis system and using method thereof Download PDF

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CN107540100B
CN107540100B CN201710970270.9A CN201710970270A CN107540100B CN 107540100 B CN107540100 B CN 107540100B CN 201710970270 A CN201710970270 A CN 201710970270A CN 107540100 B CN107540100 B CN 107540100B
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sewage
biogel
low
activated sludge
bacteria
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CN107540100A (en
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张文龙
顾金飞
李轶
张弛
赵瑞琪
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Hohai University HHU
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Abstract

The invention discloses an OUR-based activated sludge bulking early warning analysis system, which comprises a sewage filter, a sewage pump, an aeration tank, an aeration device, a closed reaction tank, a separation clapboard, a plurality of biogels, DO electrode probes corresponding to the biogels in number, a PLC control early warning system and a water outlet, the water inlet of the sewage filter is connected with the water intake on the connecting pipeline of the pretreatment system and the biochemical tank, the sewage pump is respectively connected with the sewage filter and the aeration tank, the water outlet of the aeration tank is connected with the closed reaction tank, the biological gel comprises filamentous fungi, zoogloea, low temperature, low pH, low N, low P, high H2S and biological gel of multiple soluble organic matters, the biological gel is respectively placed in different partitioned areas, the DO electrode probe is adopted for detection, and a water outlet of the closed reaction tank is connected with the pretreatment system. The invention has the functions of real-time and multi-stage early warning for sewage and real-time analysis for sludge bulking cause.

Description

OUR-based activated sludge bulking early warning analysis system and using method thereof
Technical Field
The invention relates to an OUR-based activated sludge bulking early warning analysis system and a using method thereof, belonging to the technical field of sewage treatment activated sludge imbalance real-time early warning.
Background
With the development of economic society and particularly industry in China, a large amount of industrial wastewater which is not properly treated and domestic sewage are mixed and discharged into an urban sewage treatment plant, so that the fluctuation of the quality of inlet water is caused, and the impact is brought to the stable operation of the treatment process. Especially, the existing urban sewage treatment plants in China widely adopt an activated sludge method, and the activated sludge system can cause the problems of unbalance failure, sludge expansion, sludge disintegration and the like due to the impact of toxic and harmful substances contained in sewage, so that the real-time early warning of the fluctuation of the quality of inlet water is very necessary. The conventional detection of the physicochemical indexes of water quality and sludge is time-consuming and labor-consuming, the early warning is not timely and accurate, and the online biotoxicity detection technology can quickly monitor and analyze the water inlet toxicity and degree of a sewage plant and has good research and application values.
On-line biotoxicity detection techniques are used to monitor the level of toxic organisms as individual (fish, fleas, algae), microbial (luminescent bacteria, nitrifying bacteria), sub-cellular (enzymes, DNA) and microbiota shedding. The traditional biological toxicity monitoring period of fish, flea and algae is long, the sensitivity is not high, the detection precision of enzyme and DNA sensors is high, but the preparation and the storage are difficult and the cost is high, the toxicity is easy to store and culture by utilizing the microbial detection, the enzyme action process is combined, the cost is low, the practicability is high, and the toxicity monitoring of luminous bacteria and nitrobacteria which are most widely researched and applied can reflect the toxicity of water quality to a certain extent, but the poisoning condition of the luminous bacteria and nitrobacteria to active sludge in a sewage treatment plant can not be well reflected. Therefore, research on an online biological toxicity detection technology based on the microbial community water level becomes a hotspot.
The current research and application is widely characterized in that the respiration rate (OUR) of an activated sludge system is used for representing the activity of an activated sludge microbial community, and the biotoxicity of the sewage can be known through measuring and analyzing the change of the OUR of the activated sludge. However, the existing OUR-based real-time sewage toxicity early warning system has the following defects and shortcomings:
(1) the existing OUR biological toxicity monitoring system has incomplete early warning, and only can early warn the condition that the respiration rate of an activated sludge system is reduced due to sewage, but the influence of the fluctuation of the water quality on the sludge not only inhibits all microbial communities of the sludge, but also can cause the unbalance of activated sludge microorganisms. For example, the water quality of the sewage may change to promote excessive growth of filamentous fungi, the respiration rate of the whole activated sludge system may not change or even increase, but the activated sludge system has already expanded to cause the water quality to be poor.
(2) The existing OUR biological toxicity monitoring system takes water from a biochemical pool, and sewage enters the biochemical pool, so that early warning is not timely. The extraction of sludge from biochemical tanks also has a certain effect on the morphology of the activated sludge system, the activity of microorganisms.
Disclosure of Invention
The invention aims to solve the technical problem of providing an activated sludge bulking early warning and analyzing device for a sewage plant, which can perform real-time and multi-stage early warning on sewage and perform real-time analysis on a sludge bulking cause, and a using method thereof.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
an activated sludge bulking early warning analysis system based on OUR comprises a sewage filter, a sewage pump, an aeration tank, an aeration device, a closed reaction tank, a separation partition plate, a plurality of biogels, DO electrode probes corresponding to the biogels in quantity, a PLC control early warning system and a water outlet, wherein a water inlet of the sewage filter is connected with water inlets on connecting pipelines of a pretreatment system and a biochemical tank, one end of the sewage pump is connected with the sewage filter, the other end of the sewage pump is connected with a water inlet of the aeration tank, the aeration device is arranged in the aeration tank, a water outlet of the aeration tank is connected with the closed reaction tank, the separation partition plate is used for separating the closed reaction tank into at least 8 separation areas, and the biogels comprise filamentous fungus biogels, zooglean biogel, low-temperature biogels, low-pH biogels, low-N biogels, The biological gel comprises low-P biological gel, high-H2S biological gel and multi-soluble organic matter biological gel, wherein different biological gels are used for being placed in different partitioned areas partitioned by the closed reaction tank, one DO electrode probe is respectively placed in each partitioned area, all the DO electrode probes are connected with the PLC control early warning system, and a water outlet of the closed reaction tank is connected with the pretreatment system.
The biological gel is prepared by embedding microorganisms in gel formed by mixing organic carriers by adopting an embedding method, and the prepared biological gel is respectively placed at the bottoms of different closed reaction tanks after being wetted by buffer solution.
The preparation method of the biological gel comprises the following steps:
carrying out enrichment culture, separation and purification and identification on bacteria required in activated sludge in a sewage treatment plant;
centrifuging the cultured 1600-2000 mL bacterial suspension at the rotating speed of 5000-7500 r/min for 5-10min, pouring out the supernatant, and then adding a buffer solution with the pH value of 6-10 mLpH7.0;
weighing 8-10 g of polyvinyl alcohol, 0.6-0.8 g of sodium alginate and 40-50 g of CaCl2And 1.0-1.4 g NaNO3Adding 50-60 mL of ammonia-free water, heating and stirring until the ammonia-free water is completely dissolved;
mixing the cooled mixed solution and the required bacterial concentrated solution according to the volume of 1:1, pouring the mixed solution into a mould, standing the mixture for 1 to 1.5 hours in a dark place, taking out the gel, and washing the gel twice by using non-ammonia water.
The bacteria in the filamentous bacteria biogel comprise zoogloea cladinosa, bacillus cereus, flavobacterium, nocardia actinomycetemcomitans, escherichia intermedia, pseudomonas and yeast; the bacteria in the zoogloea biogel comprise Type microfilaria, sphaerotheca, thiomyces sericata, Type0041, 0092 and 021N; the bacteria in the low-temperature biogel comprise microfilaria and Type 0041; the bacteria in the low pH biogel comprise fungal filamentous bacteria; the bacteria in the low N biogel comprise Type021N and Thiothrix II; the bacteria in the low P biogel include n.limicola iii; said high H2The bacteria in the S biological gel comprise the thiobacillus serinus and the Type 021N; the bacteria in the multi-soluble organic matter biogel comprise microfilaria and Type 0092; the bacteria in the low DO biogel include chlamydomonas.
An application method of an activated sludge bulking early warning analysis system based on OUR comprises the following steps:
starting the sewage pump, pumping sewage which is about to enter the biochemical pool after pretreatment from a water intake on a connecting pipeline of the pretreatment system and the biochemical pool, wherein the sewage firstly passes through the sewage filter for removing impurities contained in the sewage and then enters the aeration pool for pre-aeration, and the sewage is used for eliminating the influence of the change of dissolved oxygen in the sewage on the respiration rate of filamentous bacteria and zoogloea;
the sewage after pre-aeration enters the closed reaction tank from the outlet of the aeration tank, when the preset sewage amount is reached, the inlet of the closed reaction tank is closed, the separation partition plate is arranged in the closed reaction tank, the prepared filamentous fungus biogel and the zoogloea biogel are respectively arranged at the bottoms of different separation areas, and the filamentous fungus biogel and the zoogloea biogel carry out metabolic respiration by utilizing DO and nutrients in the sewage;
the DO electrode probes arranged in different divided areas measure the DO values D of the filamentous fungi and the zoogloea at the beginning and the end of the reaction0And D1The data is transmitted to the PLC control early warning system, the PLC control early warning system calculates the respiration rates of the filamentous fungi and the zoogloea according to a preset control program to carry out real-time comparison and analysis, and judges whether to give an alarm or not and the level of the alarm;
when the filamentous bacterium activated sludge bulking alarm is sent out, the PLC control system sends out a control instruction to enable the low-temperature biogel, the low-pH biogel, the low-N biogel, the low-P biogel and the high-H biogel to be in contact with the low-temperature biogel2S biogel, the multiple soluble organic matter biogel and the low DO biogel are respectively arranged in different partitioned areas, corresponding DO electrode probes are immersed below the sewage liquid level, and the DO electrode probes transmit measured real-time data to the PLC control early warning system;
the PLC control early warning system calculates the breathing rates of the filamentous bacteria which are dominant in different environments according to a preset expansion cause analysis control program to perform real-time comparative analysis, obtains factors leading activated sludge expansion and implements corresponding control measures;
and opening a water outlet of the closed reaction tank, and refluxing the sewage into the pretreatment system.
The specific steps of a control program preset by the PLC control early warning system are as follows:
(1) OUR = (D) according to a preset formula based on DO data measured by the DO electrode probe0- D1) The OUR is the respiration rate of the microorganism in the time T, and the respiration rates of the filamentous bacteria and the zoogloea are respectively calculated1And OUR2；
(2) According to the previous experiment, the normal respiration rate of the filamentous fungi entering the normal sewage is set to OURs, and the normal respiration rate of the zoogloea is set to OURJCalculating the real-time respiratory rate inhibition rate Ps and P of the filamentous bacteria/zoogloea according to a formulaJWherein the negative number represents the promotion rate, and the formula is Ps = (OURs-OUR)1）/ OURs，PJ=（OURJ-OUR2）/OURJ；
(3) According to the comparison and analysis of the respiratory rate and the inhibition rate of the filamentous fungi/zoogloea, the following early warning levels are set:
a. when the absolute Ps absolute is less than 10 percent and the absolute PJ absolute is less than 10 percent, no alarm is given, which indicates that the inlet water of the sewage plant does not fluctuate at the moment;
b. when Ps is more than 35 percent and PJ is more than 35 percent, an alarm I is sent out, which indicates that the biological toxicity in the sewage is very strong and destructive impact is brought to the activated sludge, and the sewage is stopped to enter the biochemical reaction tank immediately, so that the activated sludge system is collapsed;
c. when Ps is less than-10 percent and PJ is less than-10 percent, an alarm II is sent out, which indicates that the sewage load is too high and the aeration quantity needs to be increased or the retention time of the sewage in the biochemical tank needs to be prolonged;
d. under the other conditions except a, b and c, when Ps-PJ is more than 25%, a third alarm is sent out, which indicates that the sewage has stronger inhibition on filamentous bacteria, zoogloea in the activated sludge can take advantage, so that the activated sludge is loose and fragile, flocs are easy to break to form needle-shaped floc sludge or non-filamentous bacteria swell, the treated water quality does not reach the standard, the relevant operation process needs to be changed by analyzing reasons, and medicaments are added to solve the problem;
e. under the other conditions except a, b and c, when PJ-Ps is more than 25%, an alarm IV is sent out, which shows that the sewage has stronger inhibition on zoogloea, filamentous fungi in the activated sludge can take advantage, so that the activated sludge expands, the floc settling performance is poor, the treated water quality does not reach the standard, the relevant operation process needs to be changed by analyzing reasons, and a flocculating agent and a medicament are added to solve the problem;
f. when the absolute value Ps-PJ is less than 25%, an alarm is given, sewage has certain influence on the respiration rate of filamentous bacteria and zoogloea, has certain influence on the balance of the filamentous bacteria and the zoogloea, and needs to pay close attention to prevent the situation from deteriorating;
when an alarm is given out, the PLC control system runs an expansion cause analysis control program, and the method comprises the following specific steps:
subjecting said cryobiogel, said low pH biogel, said low N biogel, said low P biogel, said high H biogel2S biogel, the multiple soluble organic matter biogel and the low DO biogel are respectively arranged in different partitioned areas, corresponding DO electrode probes are immersed below the sewage liquid level, and the DO electrode probes transmit measured real-time data to the PLC control early warning system;
OUR = (D) according to a preset formula based on DO data measured by a DO electrode probe0- D1) The OUR is the respiration rate of the microorganism in the time T, and the respiration rates OUR of different types of filamentous bacteria are respectively calculatednN is 1-6 and represents low temperature, low pH, low N, low P and high H respectively2S, filamentous bacteria which are dominant in the environment with multiple soluble organic matters;
according to the previous experiment, the normal respiration rate of filamentous bacteria which are dominant in different environments is set to OUR0nN is 1-6, and the promotion rate Y of the real-time respiration rate of the filamentous bacteria which are dominant in different environments is calculated according to a formulanWherein negative numbers represent inhibition rates, and the formula is Yn=（OURn- OUR0n）/ OUR0n；
Based on a comparison and analysis of the respiratory rate and its inhibition rate of the different environmentally dominant filamentous bacteria, the following warnings are set:
a. rate of promotion of real-time respiration rate Y of filamentous fungus when only one or more environments are dominantnIf the water quality fluctuation is more than 20 percent, the corresponding environmental condition or conditions in the sewage promote the occurrence of activated sludge bulking, and the PLC controls the early warning system to send out an instruction to implement corresponding control measures, so that the influence of the water quality fluctuation of the sewage on an activated sludge system is reduced;
b. promotion rate Y of real-time respiration rate of filamentous bacteria when various environments are dominantnPositive, real-time respiration rate Y of one or more environmentally dominant filamentous funginY than the othersnMore than 15 percent of the total amount of the active sludge is larger than the total amount of the active sludge, which indicates that the environmental condition or the environmental conditions of the sewage are the main reason for the occurrence of the activated sludge bulking, and the PLC control early warning system sends out instructions to preferentially take measures to control the environmental conditions, so that the influence on an activated sludge system is reduced.
The invention achieves the following beneficial effects:
1. monitoring the respiration rate changes of different types of filamentous fungi and zoogloea in real time by utilizing a DO electrode and a PLC system, analyzing and researching whether the alarm is given out to cause sludge bulking or not, analyzing and obtaining main reasons causing the sludge bulking and giving control measures, and the method has the advantages of visual effect, quick early warning, stable operation, convenient operation, high efficiency and economy;
2. the sewage pump for the equipment is used for pumping sewage which is about to enter the biochemical tank after pretreatment from a water intake of a connecting pipeline of the pretreatment system and the biochemical tank, and timely early warning can be carried out to prevent toxic sewage from entering the biochemical tank to damage an activated sludge system.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the bio-gel and DO electrode probe installation of the present invention.
Detailed Description
The invention is further described below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
As shown in figures 1 and 2, the OUR-based activated sludge bulking early warning analysis system comprises a sewage filter 1, a sewage pump 2, an aeration tank 3, an aeration device, a closed reaction tank 6, a separation partition plate 7, 8 biological gels 8, DO electrode probes 9 corresponding to the biological gels 8 in quantity, a PLC (programmable logic controller) control early warning system 10 and a water outlet 11, wherein a water inlet of the sewage filter 1 is connected with a water intake on a connecting pipeline of the pretreatment system and the biochemical tank, one end of the sewage pump 2 is connected with the sewage filter 1, the other end of the sewage pump is connected with a water inlet of the aeration tank 3, an aeration pipe 4 is arranged in the aeration tank 3, the aeration pipe 4 is connected with an aerator 5, a water outlet of the aeration tank 3 is connected with the closed reaction tank 10, the separation partition plate 7 is used for separating the closed reaction tank 6 into 8 separation areas, and the biological gels 8 comprise filamentous bacterium biological gels, zoogleae, The biological gel comprises low-pH biological gel, low-N biological gel, low-P biological gel, high-H2S biological gel and multi-soluble organic matter biological gel, wherein different biological gels 8 are used for being placed in different partitioned areas partitioned by a closed reaction tank 6, a DO electrode probe 9 is respectively placed in each partitioned area, all the DO electrode probes 9 are connected with a PLC control early warning system 10, and a water outlet 11 of the closed reaction tank 10 is connected with a pretreatment system.
The biological gel is prepared by embedding microorganisms in gel formed by mixing organic carriers by adopting an embedding method, and the prepared biological gel is respectively placed at the bottoms of different closed reaction tanks 6 after being wetted by buffer solution.
The bacteria in the filamentous bacteria biogel include Acinetobacter, Bacillus cereus, Flavobacterium, Nocardia actinomycete, Escherichia intermedia, Pseudomonas, and yeast; the bacteria in the zoogloea biogel comprise Type microfilaria, sphaerotheca, thiomyces sericata, Type0041, 0092 and 021N; the bacteria in the cryobiogel include microfilaria and Type 0041; the bacteria in the low pH biogel include fungal filamentous bacteria; the bacteria in the low N biogel include Type021N and Thiothrix II; bacteria in the low P biogel include n.limicola iii; high H2The bacteria in the S biological gel comprise the thiobacillus serinus and the Type 021N; the bacteria in the multi-soluble organic matter biogel comprise microfilaria and Type 0092; the bacteria in the low DO biogel include chlamydomonas.
The preparation method of the biological gel comprises the following steps:
carrying out enrichment culture, separation and purification and identification on bacteria required in activated sludge in a sewage treatment plant;
centrifuging the cultured 1600-2000 mL required bacterial suspension for 5-10min at the rotating speed of 5000-7500 r/min, pouring out the supernatant, and then adding 6-10 mL buffer solution with pH7.0;
8-10 g of polyvinyl alcohol, 0.6-0.8g of sodium alginate and 40-50 g of CaCl2And 1.0-1.4 g NaNO3Adding 50-60 mL of ammonia-free water, heating and stirring until the ammonia-free water is completely dissolved;
mixing the cooled mixed solution and the required bacterial concentrated solution according to the volume of 1:1, pouring the mixed solution into a mould, keeping the mould away from light, standing the mould for 1 to 1.5 hours, taking out the membrane, and washing the membrane twice by using non-ammonia water.
An application method of an activated sludge bulking early warning analysis system based on OUR comprises the following steps:
starting a sewage pump 2, pumping sewage which is about to enter a biochemical tank after pretreatment from a water intake on a connecting pipeline of a pretreatment system and the biochemical tank, enabling the sewage to pass through a sewage filter 1 for removing impurities contained in the sewage, then entering an aeration tank 3 for pre-aerating the sewage, eliminating the influence of dissolved oxygen change in the sewage on the respiration rate of filamentous bacteria and zoogloea, aerating the sewage by an aerator 5 through an aeration pipe 4 to enable the DO concentration to reach 2.5-2.8 mg/L, and then stopping aeration;
the sewage after pre-aeration enters a closed reaction tank 6 from an outlet of an aeration tank 3, when the preset sewage amount is reached, an inlet of the closed reaction tank 6 is closed, a separation baffle 7 is arranged in the closed reaction tank 6, the prepared filamentous fungus biogel and zoogloea biogel are respectively arranged at the bottoms of different separation areas, the filamentous fungus biogel and the zoogloea biogel carry out metabolic respiration by utilizing DO and nutrients in the sewage, and the DO concentration is gradually reduced along with the prolonging of the reaction time. According to related experiments, the DO concentration is found to be reduced most obviously within 5-10min, so that the reaction time of the closed reaction tank is 6 min;
the DO electrode probes 9 arranged in different partitioned areas measure the DO values D of the filamentous fungi and the zoogloea at the beginning and the end of the reaction0And D1The data is transmitted to the PLC control early warning system 10, the PLC control early warning system 10 calculates the respiration rates of the filamentous fungi and the zoogloea according to a preset control program to carry out real-time comparison and analysis, and judges whether to give an alarm or not and the level of the alarm;
when the filamentous bacterium activated sludge bulking alarm is sent out, the PLC controlsThe system 10 issues control commands to apply cryobiogel, low pH biogel, low N biogel, low P biogel, high H biogel2Respectively placing S biogel, multi-soluble organic matter biogel and low-DO biogel in different partitioned areas, immersing the corresponding DO electrode probe 9 below the sewage level, and transmitting the measured real-time data to the PLC control early warning system 10 by the DO electrode probe 9;
the PLC control early warning system 10 calculates the breathing rates of the filamentous bacteria which are dominant in different environments according to a preset expansion cause analysis control program to perform real-time comparative analysis, obtains factors leading activated sludge expansion and implements corresponding control measures;
and opening a water outlet 11 of the closed reaction tank 6, and refluxing the sewage into the pretreatment system.
The specific steps of a control program preset by the PLC control early warning system 10 are as follows:
(1) the DO data measured by a DO electrode probe is OUR = D according to a preset formula0- D1The OUR is the respiration rate of the microorganism in the time T, and the respiration rates of the filamentous bacteria and the zoogloea are respectively calculated1And OUR2；
(2) According to the previous experiment, the normal respiration rate of the filamentous fungi entering the normal sewage is set to OURs, and the normal respiration rate of the zoogloea is set to OURJCalculating the real-time respiratory rate inhibition rate Ps and P of the filamentous bacteria/zoogloea according to a formulaJWherein the negative number represents the promotion rate, and the formula is Ps = OURs-OUR1/ OURs，PJ=OURJ-OUR2/OURJ；
(3) According to the comparison and analysis of the respiratory rate and the inhibition rate of the filamentous fungi/zoogloea, the following early warning levels are set:
a. when the absolute Ps absolute is less than 10 percent and the absolute PJ absolute is less than 10 percent, no alarm is given, which indicates that the inlet water of the sewage plant does not fluctuate at the moment;
b. when Ps is more than 35 percent and PJ is more than 35 percent, an alarm I is sent out, which indicates that the biological toxicity in the sewage is very strong and destructive impact is brought to the activated sludge, and the sewage is stopped to enter the biochemical reaction tank immediately, so that the activated sludge system is collapsed;
c. when Ps is less than-10 percent and PJ is less than-10 percent, an alarm II is sent out, which indicates that the sewage load is too high and the aeration quantity needs to be increased or the retention time of the sewage in the biochemical tank needs to be prolonged;
d. under the other conditions except a, b and c, when Ps-PJ is more than 25%, a third alarm is sent out, which indicates that the sewage has stronger inhibition on filamentous bacteria, zoogloea in the activated sludge can take advantage, so that the activated sludge is loose and fragile, flocs are easy to break to form needle-shaped floc sludge or non-filamentous bacteria swell, the treated water quality does not reach the standard, the relevant operation process needs to be changed by analyzing reasons, and medicaments are added to solve the problem;
e. under the other conditions except a, b and c, when PJ-Ps is more than 25%, an alarm IV is sent out, which shows that the sewage has stronger inhibition on zoogloea, filamentous fungi in the activated sludge can take advantage, so that the activated sludge expands, the floc settling performance is poor, the treated water quality does not reach the standard, the relevant operation process needs to be changed by analyzing reasons, and a flocculating agent and a medicament are added to solve the problem;
f. when the absolute value Ps-PJ is less than 25%, an alarm is given, sewage has certain influence on the respiration rate of filamentous bacteria and zoogloea, has certain influence on the balance of the filamentous bacteria and the zoogloea, and needs to pay close attention to prevent the situation from deteriorating;
(4) when the third alarm is given, the PLC control system 10 runs the expansion cause analysis control program, which includes the following specific steps:
mixing low temperature biogel, low pH biogel, low N biogel, low P biogel, high H biogel2Respectively placing S biogel, multi-soluble organic matter biogel and low-DO biogel in different partitioned areas, immersing the corresponding DO electrode probe 9 below the sewage level, and transmitting the measured real-time data to the PLC control early warning system 10 by the DO electrode probe 9;
the DO data measured by a DO electrode probe is OUR = D according to a preset formula0- D1The OUR is the respiration rate of the microorganism in the time T, and the respiration rates OUR of different types of filamentous bacteria are respectively calculatednN is 1-6 and represents low temperature, low pH, low N, low P and high H respectively2S, can be dissolved inA predominant filamentous fungus in an organic environment;
according to the previous experiment, the normal respiration rate of filamentous bacteria which are dominant in different environments is set to OUR0nN is 1-6, and the promotion rate Y of the real-time respiration rate of the filamentous bacteria which are dominant in different environments is calculated according to a formulanWherein negative numbers represent inhibition rates, and the formula is Yn=OURn- OUR0n/ OUR0n；
Based on a comparison and analysis of the respiratory rate and its inhibition rate of the different environmentally dominant filamentous bacteria, the following warnings are set:
a. rate of promotion of real-time respiration rate Y of filamentous fungus when only one or more environments are dominantnIf the water quality fluctuation is more than 20 percent, the corresponding environmental condition or conditions in the sewage promote the occurrence of activated sludge bulking, and the PLC control early warning system 10 sends out an instruction to implement corresponding control measures, so that the influence of the water quality fluctuation of the sewage on an activated sludge system is reduced;
b. promotion rate Y of real-time respiration rate of filamentous bacteria when various environments are dominantnPositive, real-time respiration rate Y of one or more environmentally dominant filamentous funginY than the othersnMore than 15 percent of the total amount of the activated sludge is larger than the total amount of the activated sludge, which indicates that the environmental condition or the environmental conditions of the sewage are the main reason for the occurrence of the activated sludge bulking, and the PLC control early warning system 10 sends out instructions to preferentially take measures to control the environmental conditions, thereby reducing the influence on the activated sludge system.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.

Claims (6)

1. An OUR-based activated sludge bulking early warning analysis system is characterized by comprising a sewage filter (1), a sewage pump (2), an aeration tank (3), an aeration device, a closed reaction tank (6), a separation partition plate (7), a plurality of biogels (8), DO electrode probes (9) corresponding to the biogels (8), a PLC control early warning system (10) and a water outlet (11), wherein a water inlet of the sewage filter (1) is connected with water inlets on connecting pipelines of a pretreatment system and a biochemical tank, one end of the sewage pump (2) is connected with the sewage filter (1), the other end of the sewage pump is connected with a water inlet of the aeration tank (3), the aeration device is arranged in the aeration tank (3), a water outlet of the aeration tank (3) is connected with the closed reaction tank (6), the separation partition plate (7) is used for separating the closed reaction tank (6) into at least 8 separation areas, the biological gel (8) comprises filamentous fungus biological gel, zoogloea biological gel, low-temperature biological gel, low-pH biological gel, low-N biological gel, low-P biological gel, high-H2S biological gel and multi-soluble organic matter biological gel, different biological gels (8) are used for being placed in different separation areas separated by the closed reaction tank (6), one DO electrode probe (9) is respectively placed in each separation area, all the DO electrode probes (9) are connected with the PLC control early warning system (10), and a water outlet (11) of the closed reaction tank (10) is connected with the pretreatment system.
2. The OUR-based activated sludge bulking pre-warning and analyzing system as claimed in claim 1 wherein the bio-gel is embedded with microbes in a gel formed by mixing organic carriers by an embedding method, and the prepared bio-gel is wetted with a buffer solution and then placed at the bottom of different closed reaction cells (6) respectively.
3. The OUR-based activated sludge bulking pre-warning and analyzing system of claim 2 wherein the bio-gel is prepared by the following steps:
carrying out enrichment culture, separation and purification and identification on bacteria required in activated sludge in a sewage treatment plant;
centrifuging the cultured 1600-2000 mL bacterial suspension for 5-10min at the rotating speed of 5000-7500 r/min, pouring out the supernatant, and then adding 6-10 mL buffer solution with pH7.0;
weighing 8-10 g of polyvinyl alcohol, 0.6-0.8 g of sodium alginate and 40-50 g of CaCl2And 1.0-1.4 g NaNO3Adding 50-60 mL of ammonia-free water, heating and stirring until the ammonia-free water is completely dissolved;
mixing the cooled mixed solution and the required bacterial concentrated solution according to the volume of 1:1, pouring the mixed solution into a mould, standing the mixture for 1 to 1.5 hours in a dark place, taking out the gel, and washing the gel twice by using non-ammonia water.
4. The OUR-based activated sludge bulking pre-warning analysis system of claim 1 wherein the bacteria in the filamentous bacterial biogel include zoogloea, Bacillus cereus, Flavobacterium, Nocardia actinomycetemcomitans, Escherichia intermedia, Pseudomonas, and yeast; the bacteria in the zoogloea biogel comprise Type microfilaria, sphaerotheca, thiomyces sericata, Type0041, 0092 and 021N; the bacteria in the low-temperature biogel comprise microfilaria and Type 0041; the bacteria in the low pH biogel comprise fungal filamentous bacteria; the bacteria in the low N biogel include Type021N and Thiothrix ii; the bacteria in the low P biogel include n.limicola iii; said high H2The bacteria in the S biological gel comprise the thiobacillus serinus and the Type 021N; the bacteria in the multi-soluble organic matter biogel comprise Microserium and Type 0092.
5. The use method of the OUR-based activated sludge bulking pre-warning analysis system of claim 1, comprising the steps of:
starting the sewage pump (2), pumping sewage which is about to enter the biochemical tank after pretreatment from a water intake on a connecting pipeline of the pretreatment system and the biochemical tank, wherein the sewage firstly passes through the sewage filter (1) for removing impurities contained in the sewage and then enters the aeration tank (3) for pre-aeration of the sewage, and is used for eliminating the influence of the change of dissolved oxygen in the sewage on the respiration rate of filamentous bacteria and zoogloea;
the sewage after pre-aeration enters the closed reaction tank (6) from the outlet of the aeration tank (3), when the preset sewage amount is reached, the inlet of the closed reaction tank (6) is closed, the separation partition plate (7) is arranged in the closed reaction tank (6), the prepared filamentous fungus biogel and the zoogloea biogel are respectively arranged at the bottoms of different separation areas, and the filamentous fungus biogel and the zoogloea biogel carry out metabolic respiration by utilizing DO and nutrients in the sewage;
the DO electrode probes (9) arranged in different separation areas measure the DO values D of the filamentous fungi and the zoogloea at the beginning and the end of the reaction0And D1The data are transmitted to the PLC control early warning system (10), the PLC control early warning system (10) calculates the respiration rates of the filamentous fungi and the zoogloea according to a preset control program to carry out real-time comparison and analysis, and judges whether to give an alarm or not and the level of the alarm;
when filamentous activated sludge bulking alarm is given out, the PLC control system (10) sends out a control instruction to enable the low-temperature biogel, the low-pH biogel, the low-N biogel, the low-P biogel and the high-H biogel to be in contact with the low-temperature biogel2S biogel and the multiple soluble organic matter biogel are respectively arranged in different partitioned areas, the corresponding DO electrode probe (9) is immersed below the sewage level, and the DO electrode probe (9) transmits measured real-time data to the PLC control early warning system (10);
the PLC control early warning system (10) calculates the breathing rates of the filamentous bacteria which are dominant in different environments according to a preset expansion cause analysis control program to perform real-time comparative analysis, obtains factors leading activated sludge expansion and implements corresponding control measures;
and opening a water outlet (11) of the closed reaction tank (6), and refluxing the sewage into the pretreatment system.
6. The use method of the OUR-based activated sludge bulking pre-warning analysis system according to claim 5, wherein the PLC control pre-warning system (10) is preset with a control program comprising the following steps:
OUR = (D) according to a preset formula based on DO data measured by the DO electrode probe0- D1) The OUR is the respiration rate of the microorganism in the time T, and the respiration rates of the filamentous bacteria and the zoogloea are respectively calculated1And OUR2；
According to the previous experiment, the normal respiration rate of the filamentous fungi entering the normal sewage is set to OURs, and the normal respiration rate of the zoogloea is set to OURJCalculating the real-time respiratory rate inhibition rate Ps and P of the filamentous bacteria/zoogloea according to a formulaJWherein the negative number represents the promotion rate, and the formula is Ps = (OURs-OUR)1）/ OURs，PJ=（OURJ-OUR2）/OURJ；
According to the comparison and analysis of the respiratory rate and the inhibition rate of the filamentous fungi/zoogloea, the following early warning levels are set:
a. when the absolute Ps absolute is less than 10 percent and the absolute PJ absolute is less than 10 percent, no alarm is given, which indicates that the inlet water of the sewage plant does not fluctuate at the moment;
b. when Ps is more than 35 percent and PJ is more than 35 percent, an alarm I is sent out, which indicates that the biological toxicity in the sewage is very strong and destructive impact is brought to the activated sludge, and the sewage is stopped to enter the biochemical reaction tank immediately, so that the activated sludge system is collapsed;
c. when Ps is less than-10 percent and PJ is less than-10 percent, an alarm II is sent out, which indicates that the sewage load is too high and the aeration quantity needs to be increased or the retention time of the sewage in the biochemical tank needs to be prolonged;
d. under the other conditions except a, b and c, when Ps-PJ is more than 25%, a third alarm is sent out, which indicates that the sewage has stronger inhibition on filamentous bacteria, zoogloea in the activated sludge can take advantage, so that the activated sludge is loose and fragile, flocs are easy to break to form needle-shaped floc sludge or non-filamentous bacteria swell, the treated water quality does not reach the standard, the relevant operation process needs to be changed by analyzing reasons, and medicaments are added to solve the problem;
e. under the other conditions except a, b and c, when PJ-Ps is more than 25%, an alarm IV is sent out, which shows that the sewage has stronger inhibition on zoogloea, filamentous fungi in the activated sludge can take advantage, so that the activated sludge expands, the floc settling performance is poor, the treated water quality does not reach the standard, the relevant operation process needs to be changed by analyzing reasons, and a flocculating agent and a medicament are added to solve the problem;
f. when the absolute value Ps-PJ is less than 25%, an alarm is given, sewage has certain influence on the respiration rate of filamentous bacteria and zoogloea, has certain influence on the balance of the filamentous bacteria and the zoogloea, and needs to pay close attention to prevent the situation from deteriorating;
when the alarm III is given out, the PLC control system (10) runs an expansion cause analysis control program, and the method comprises the following specific steps:
subjecting said cryobiogel, said low pH biogel, said low N biogel, said low P biogel, said high H biogel2S biogel, the multi-soluble organic matter biogel and the low-DO biogel are respectively arranged in different partitioned areas, corresponding DO electrode probes (9) are immersed below the sewage liquid level, and the DO electrode probes (9) transmit measured real-time data to the PLC control early warning system (10);
OUR = (D) according to a preset formula based on DO data measured by a DO electrode probe0- D1) The OUR is the respiration rate of the microorganism in the time T, and the respiration rates OUR of different types of filamentous bacteria are respectively calculatednN is 1-6 and represents low temperature, low pH, low N, low P and high H respectively2S, filamentous bacteria which are dominant in the environment with multiple soluble organic matters;
according to the previous experiment, the normal respiration rate of filamentous bacteria which are dominant in different environments is set to OUR0nN is 1-6, and the promotion rate Y of the real-time respiration rate of the filamentous bacteria which are dominant in different environments is calculated according to a formulanWherein negative numbers represent inhibition rates, and the formula is Yn=（OURn- OUR0n）/ OUR0n；
Based on a comparison and analysis of the respiratory rate and its inhibition rate of the different environmentally dominant filamentous bacteria, the following warnings are set:
a. rate of promotion of real-time respiration rate Y of filamentous fungus when only one or more environments are dominantnMore than 20 percent, indicates the corresponding environmental condition or conditions in the sewage and promotes the activated sludge to expandWhen the expansion occurs, the PLC controls the early warning system (10) to send out an instruction to implement a corresponding control measure, so that the influence of the water quality fluctuation of the sewage on the activated sludge system is reduced;
b. promotion rate Y of real-time respiration rate of filamentous bacteria when various environments are dominantnPositive, real-time respiration rate Y of one or more environmentally dominant filamentous funginY than the othersnMore than 15 percent of the total amount of the active sludge is larger than the total amount of the active sludge, which indicates that the environmental condition or the environmental conditions of the sewage are the main reason for the occurrence of the activated sludge bulking, and the PLC control early warning system (10) sends an instruction to preferentially take measures to control the environmental conditions, so that the influence on an activated sludge system is reduced.
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JPH0471686A (en) * 1990-07-11 1992-03-06 Nippon Health Kogyo Kk Method for killing actinomyces and filamentous fungus in treated waste water produced by secondary treatment
CN101759333A (en) * 2010-02-10 2010-06-30 彭永臻 Apparatus for stably maintaining sludge micro-bulking for saving energy in biological denitrification technology and method thereof
CN101907596A (en) * 2010-08-30 2010-12-08 重庆大学 Method for monitoring and estimating inlet water toxicity of urban wastewater treatment plant on line
CN104914213A (en) * 2015-05-13 2015-09-16 西安建筑科技大学 Method for early warning of activated sludge filamentous bacterium expansion

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JPH0471686A (en) * 1990-07-11 1992-03-06 Nippon Health Kogyo Kk Method for killing actinomyces and filamentous fungus in treated waste water produced by secondary treatment
CN101759333A (en) * 2010-02-10 2010-06-30 彭永臻 Apparatus for stably maintaining sludge micro-bulking for saving energy in biological denitrification technology and method thereof
CN101907596A (en) * 2010-08-30 2010-12-08 重庆大学 Method for monitoring and estimating inlet water toxicity of urban wastewater treatment plant on line
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