CN1075067A - The preparation method of north cordyceps sinensis beverage - Google Patents
The preparation method of north cordyceps sinensis beverage Download PDFInfo
- Publication number
- CN1075067A CN1075067A CN 92100754 CN92100754A CN1075067A CN 1075067 A CN1075067 A CN 1075067A CN 92100754 CN92100754 CN 92100754 CN 92100754 A CN92100754 A CN 92100754A CN 1075067 A CN1075067 A CN 1075067A
- Authority
- CN
- China
- Prior art keywords
- beverage
- water
- extract
- raw material
- mycelium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention is that northern Chinese caterpillar Fungus stroma, mycelium and the culture medium thereof of employing artificial culture is raw material, and effective component extracting is prepared into the method for beverage.It is characterized in that adopting above-mentioned raw materials to obtain nutritional labeling, blend into the beverage of colourless or salmon pink again through processing such as pulverizing, leach, remove slag; Or make instant crystal, thereby become a kind of new beverage through freeze drying or spray-drying.This product is all formed with natural materials, does not contain artificial color, and is nutritious, and tangible antioxidation and antibacterial action are arranged.
Description
The invention belongs to the food and drink production field.
Along with scientific and technological progress, the development of society, people increase the beverage demand, for example: 1980 to nineteen ninety China's quantity of consuming beverage per capita increased 3-8 doubly, and the trend that increases is year by year arranged.The drink variety of selling on the China market has external, as, Coca-Cola, Pepsi Cola; Joint and homemade kind are arranged, happy etc. as: Sprite, Jilibao, Mount Huang cola, self-sufficient and strategically located region mouth.Although kind is very various, lack the beverage of antioxidation aspect.The present invention utilizes modern high technology to work out a kind of new beverage.It is of high nutritive value, and is nontoxic, does not contain artificial color; Anti-oxidant, antidotal effect is arranged, the effect that suppresses free radical damage is arranged, can protect cell membrane stability; Have antiviral and antibacterial action, antitumor action is arranged, can improve immunity of organisms.It is a kind of state-of-the art science beverage.
Northern Chinese caterpillar Fungus (Cordgceps militaris) also claims northern Chinese caterpillar fungus (down together), pupa grass, Cordyceps militaris; It is the kind that rare traditional Chinese medicine Chinese caterpillar fungus (Cordyceps sinensis) belongs to.The north Chinese caterpillar fungus is for a long time medicinal, can replace the effect of Chinese caterpillar fungus to be used as medicine (national Chinese herbal medicine compilation People's Health Publisher 273-274 page or leaf in 1976).The sweet beneficial lung kidney of the flat flavor of north Chinese caterpillar fungus property is mended marrow, hemostasis and phlegm.The northern Chinese caterpillar fungus of artificial culture has same nature, does not still have report with it as the method that the raw material effective component extracting prepares beverage at present.
The objective of the invention is to create a kind of northern Chinese caterpillar Fungus stroma, mycelium and culture medium with artificial culture is raw material, makes a kind of technology of colourless or salmon pink beverage.
Technical characterictic of the present invention is: obtain active ingredient through pulverizing, immersion, homogenate, filtration (or squeezing, centrifugal) after removing slag with above-mentioned raw materials, blend into water white transparency or salmon pink beverage again.Or with beverage after ultrafiltration, reverse osmosis concentration, take freeze drying or spray-drying to make instant crystal.
Narrate technical process of the present invention below:
The beverage of manufacturing of the present invention is that northern Chinese caterpillar Fungus stroma, mycelium and the culture medium with artificial culture is raw material.With after the raw material pulverizing with water or water alcohol mixed liquid dipping effective component extracting.Stroma, mycelium and culture medium adopt dry product 25-70 gram/per hundred milliliters of leaching liquids, optium concentration is at 40-60 gram film solid media mycelium/dl, 30-50 gram/dl, 40-60 restrains mycelium/dl, when extracting, use the water of PH3.0-7.0 and be advisable, fermenting raw materials souring when preventing that temperature is high with water, optimal pH 4.0 soaked about 12-72 hour.Acid water can prevent varied bacteria growing, helps extracting.The pure soluble substance that contains in the raw material is as extracting with water-alcohol mixture or extract separately with alcohol, common water: ethanol (5: 5 or 4: 6) mixture soaks the raw material optium concentration at 40-60%, and soak time is 12-72 hour.For shortened process, 70 ℃ of heating commonly used about 1-2 hour, 100 ℃ of heating are more than 30 minutes, or pressurization 1.0-1.25 kilogram/cm
2, about 10 minutes, in order to extraction.After blending into beverage again, the preliminary extract that obtains has many common traits.One of its feature is: during amino acid analysis, compares more than 14 kinds with natural cordyceps, and its ASSOCIATE STATISTICS, coefficient correlation (r value) is greater than 0.85.When with ultraviolet spectroscopy said extracted liquid or beverage, trace absorption honeybee curve, found that at the 240nm place to form trough, form crest at the 260nm place.When analyzing with infrared spectrum, the beverage of finding this manufactured is at 3400cm
-1, 1625cm
-1And 1050cm
-1The place forms trough; At 3000cm
-1, 1500cm
-1, 1200cm
-1And 850cm
-1Near have crest to form.When analyzing with visible spectrum, it is relevant with concentration that the beverage that the present invention makes forms the absworption peak absorbance at 420nm and 440-445nm place, A
1% 1cm=1.0-1.8.445cm place absorbance and raw material color and luster relevant (above-mentioned measuring technology is assisted to finish by test center of Military Medical Science Institute) in direct ratio.
The raw material that the present invention adopts is a kind of fungal cell with edible and medical value, in order to promote the release of active ingredient, makes more than the extraction rate reached to 85%, it is characterized in that: adopt following method to carry out.(1) after the collection raw material soaking, wear into Other Instruments with colloid and make it homogenate, the method for using homogenate makes cell wall rupture discharge nutritional labeling.(2) soak is warmed up to about 50 ° and keeps promoting in 2-4 hour self enzymolysis, or the adding cellulase makes it enzymolysis.
With the method for filtering (or plate and frame squeeze and filter, or centrifugal) scum juice is separated with the northern Chinese caterpillar fungus extract of the inventive method preparation, obtain active ingredient.Edible compositions such as blending and tasting agent, sugar part do not add any artificial color.
Beverage with the inventive method production, in order to carry and to preserve, adopt concentration technique or ultrafiltration, reverse osmosis concentration, the true chamber of low temperature to concentrate, methods such as the jacketed pan heating concentrates make it concentrated, and then adopt the method for spray-drying, freeze drying or baking to make it to become salmon pink crystalline solid or white powder crystalline solid.Thereby make it to have instant characteristics.
The north cordyceps sinensis beverage that the present invention makes is characterized in that: when the mycelium that obtains with solid mycelium (comprising culture medium) or submerged fermentation is raw material, utilize the light irradiation to make the white hypha body become the salmon pink mycelium.Make beverage possess distinctive salmon pink, and the special fragrance flavor is arranged, use the white hypha body, then have the beverage of aromatic odor for water white transparency as not adopting the light irradiation.
Can prepare 1 liter of beverage with the fresh stroma of per 40 grams of said method, northern Chinese caterpillar fungus effective component extraction rate can reach more than 80%.Use above-mentioned technology and can also be prepared into serial carbonated drink or beverage, with former top grade, middle-grade with low-grade different product, can also make different flavouring additives.Chinese caterpillar fungus property flat flavor in north is sweet, and beneficial lung kidney is mended marrow, hemostasis and phlegm; In Chinese medicine, belong to top grade, similar to Chinese caterpillar fungus edible, also can be medicinal.The north cordyceps sinensis beverage of producing with the method for the invention has multiple function: (1) nutritious smell fragrance is bright in colour; (2) have antibiotic and antivirus action; (3) effect anti-oxidant and the protection cell is arranged; (4) antifatigue and cardiotonic are arranged; (5) tranquilizing and allaying excitement is arranged, strengthen the effect of immunity of organisms; (6) antitumor action is arranged; (7) effect of anti-mycobacterium tuberculosis is arranged.
It is raw material that the beverage of manufacturing of the present invention adopts the northern Chinese caterpillar fungus of artificial culture.For this reason, we at first study in great detail raw material, and the sample of cultivation is identified through Institute of Micro-biology of the Chinese Academy of Sciences and is strictly northern Chinese caterpillar fungus.Adopt high-tech to analyze.For example: the stroma that DNA RNA the analysis showed that artificial culture and natural cs, northern Chinese caterpillar fungus are similar, and artificial silkworm chrysalis Cordyceps sinensis is slightly high; One of active principle in the Chinese caterpillar fungus is a cordycepin, be 3 '-desoxyadenossine, it is that content reaches 10-400mg/L in a kind of composition beverage among the DNA.Rna content is then relevant because of culture medium or mycelial growth state difference.Near infrared spectrum 51A type infrared characteristic analyzer (6265-4241cm
-1) measure and to show that the raw material that the present invention adopts is identical with the spectral signature of wild Chinese caterpillar fungus.Infrared spectrum Fourier transform infrared spectrophotometer (4000-400cm
-1) the analysis showed that the fingerprint region Chinese caterpillar fungus similar to the infrared absorption spectroscopy of artificial cordyceps.The 557 type ultraviolet specrophotometers scanning of ultraviolet spectra Hitachi has absworption peak at 260nm; Visible light part 420 and 445nm near an absworption peak is arranged, be the absorption feature of distinctive salmon pink pigment, its color and luster is in full accord with the color and luster of wild northern Chinese caterpillar fungus maturation stroma.We study sclerotium, have done enzyme spectrum analysis, and the regrowth hair stroma that also right-20 ℃ of refrigerations formed after five months has carried out microexamination, confirms that from morphosis artificial raw material is consistent with wild raw material.Above-mentioned work there is no report through information institute of Chinese medical courses in general institute international online retrieval.In addition, through northern Chinese caterpillar fungus acute and chronic toxicity test; That teratogenesis, carcinogenic test all confirm is nontoxic, harmless, do not have carcinogenic teratogenesis.Therefore, the present invention passes through the result that the tight research of a series of science just obtains.Show that through the Green Tea Extract test its effect is identical with the red sage root, so be a kind of extremely promising raw materials for production.The technology of producing with the inventive method has kept nutritive value and the biological effect of northern Chinese caterpillar fungus, will be a kind of significant new beverage.
Production method below by a kind of north cordyceps sinensis beverage illustrates the present invention:
Example one adds 500 milliliters in water with stroma of Cordyceps militaris 50 gram of artificial culture, and pressurization was boiled 10 minutes, obtained salmon pink liquid, and inclining supernatant, adds 200 milliliters in water and repeats to extract once.Filter and remove residue merges extract.This liquid is salmon pink, and flavor fragrance is slightly pleasantly sweet, as traditional Chinese medicine introduction " the flat flavor of its property is sweet ".Blend into 5 liters of beverages with flavor enhancement and sugar.The salmon pink beverage.With the visible spectrum analysis, the absorbance at the 445nm place is about 1.2. be A
1% 1cm=1.2.
Example two is inoculated northern cordyceps species with rice medium, adopts method for tissue culture under aseptic condition, makes it to become the salmon pink mycelium with the light irradiation, and can form with the stroma oven dry back of wild northern Chinese caterpillar fungus homomorphosis standby.Take by weighing above-mentioned dry product 1000 grams, (prevent to go bad) under cryogenic conditions with light water and soaked 48 hours or soaked 72 hours with the acid water of pH4.0, water consumption is 4000 to restrain.Put into beater or colloid mill homogenate, filter, remove slag, obtain light salmon pink (or orange colour) liquid, repeat extracting and once merge extract, add flavor enhancement and sugar etc. and blend into 10 liters of beverages.
Claims (15)
1, a kind of northern Chinese caterpillar Fungus stroma, mycelium and culture medium thereof with artificial culture is the method for raw material preparation of drinks.The manufacture craft that it is characterized in that it, this method is raw materials used to obtain active ingredient through pulverizing, immersion, homogenate, filtration (or squeezing, centrifugal) after removing slag, and blends into water white transparency salmon pink beverage again.Or after ultrafiltration, reverse osmosis concentration, take freeze drying or spray-drying to make instant crystal the beverage.
2, method according to claim 1, it is characterized in that with after the raw material pulverizing with water or water alcohol mixed liquid dipping, effective component extracting.
3, method according to claim 1, it is characterized in that soaking raw material dry product consumption is 25-70 gram/per hundred milliliters of leaching liquids, solid medium soak optium concentration about the 40-60 gram/per hundred milliliters of leaching liquids.
4, according to claim 1 and 2, it is characterized in that extracting with the water of PH3.0-PH7.0, optimal pH about 4.0 is through soaking 12-72 hour acquisition extract.
5, according to claim 1 and 2, it is characterized in that water: alcohol mixture soaks 12-72 hour acquisition extract of raw material, and mixture is for containing ethanol 40-60%.
6, according to claim 1 and 2, when it is characterized in that water or water-alcohol mixture extract, for the shortening time adopts heating means to extract, optimum condition is 70 ℃ of heating about 1-2 hour, 100 ℃ more than 30 minutes or the pressurization method of boiling more than 10 minutes extract working substance.
7, according to claim 1 and 3, after it is characterized in that blending into beverage with extract, when 14 seed amino acid analyses and natural cs aminoacid ingredient ASSOCIATE STATISTICS, its correlation coefficient r＞0.85.
8, according to claim 1 and 2, the beverage that adopts the inventive method to make contains natural colored, measures with spectroscopic analysis methods, it is characterized in that the ultraviolet light part forms trough at 240nm, forms crest at 260nm.
9, according to claim 1,2 and 8 described, it is characterized in that finished beverage 3400cm in infrared spectrum analysis
-1, 1625cm
-1And 1050cm
-1The place forms trough: at 3000cm
-1, 1500cm
-1, 1200cm
-1, 850cm
-1Near have crest to form.
10,, it is characterized in that beverage in visible spectrum is analyzed, in 420nm, 440-445nm place formation absworption peak according to claim 1,2 and 8 described.
11, according to claim 1 and 2, it is characterized in that soaking the back and make cell wall rupture discharge nutrition with the homogenate method.
12, according to claim 1 and 2, it is characterized in that insulation promoted enzymolysis in 2-4 hour about 50 ℃ with being warmed up to, destroy cell membrane and discharge nutritional labeling.
13, according to claim 1, it is characterized in that not adding artificial color with the extract of said method acquisition, the blending and tasting agent, sugar is made the method for beverage.
14,, it is characterized in that making instant crystalline solid with freeze drying or spray-dired method according to claim 1 and 13 described methods.
15, according to claim 1, utilize the light irradiation to make the white hypha body become the mycelial method of salmon pink when it is characterized in that adopting mycelium that solid mycelium or submerged fermentation obtain for raw material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92100754 CN1075067A (en) | 1992-02-02 | 1992-02-02 | The preparation method of north cordyceps sinensis beverage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92100754 CN1075067A (en) | 1992-02-02 | 1992-02-02 | The preparation method of north cordyceps sinensis beverage |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1075067A true CN1075067A (en) | 1993-08-11 |
Family
ID=4938776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 92100754 Pending CN1075067A (en) | 1992-02-02 | 1992-02-02 | The preparation method of north cordyceps sinensis beverage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1075067A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0768089A1 (en) * | 1994-06-30 | 1997-04-16 | Meiji Milk Products Company Limited | Health composition |
| CN1099836C (en) * | 2000-03-23 | 2003-01-29 | 周国君 | Health-care cordyseps sweets and preparing process thereof |
| CN102168108A (en) * | 2010-12-17 | 2011-08-31 | 广东省微生物研究所 | Cordyceps cardinalis, method for preparing oosporin by using same and application of Cordyceps cardinalis |
| CN102266054A (en) * | 2011-07-26 | 2011-12-07 | 西藏月王生物技术有限公司 | Aweto ferment and preparation method thereof |
| CN102805401A (en) * | 2012-07-20 | 2012-12-05 | 黄晓青 | Chinese caterpillar fungus health-care drink prepared by liquid deep submerged fermentation and preparation method thereof |
| CN103355727A (en) * | 2013-08-06 | 2013-10-23 | 郑剑峰 | Preparation method for solid Cordyceps militaris beverage |
| CN104473977A (en) * | 2014-11-18 | 2015-04-01 | 广东省微生物研究所 | Cordyceps militaris water extract as well as preparation method and application thereof |
| CN105661240A (en) * | 2016-03-10 | 2016-06-15 | 浙江博士园生物技术有限公司 | Cordyceps militaris beverage and making method thereof |
| CN108056307A (en) * | 2017-11-30 | 2018-05-22 | 安徽壮大饲料有限公司 | A kind of laying duck seedling feed and preparation method thereof |
| CN108576504A (en) * | 2018-03-29 | 2018-09-28 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of wax gourd high microsteping beverage |
-
1992
- 1992-02-02 CN CN 92100754 patent/CN1075067A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0768089A1 (en) * | 1994-06-30 | 1997-04-16 | Meiji Milk Products Company Limited | Health composition |
| EP0768089A4 (en) * | 1994-06-30 | 1997-08-20 | Meiji Milk Prod Co Ltd | Health composition |
| CN1099836C (en) * | 2000-03-23 | 2003-01-29 | 周国君 | Health-care cordyseps sweets and preparing process thereof |
| CN102168108A (en) * | 2010-12-17 | 2011-08-31 | 广东省微生物研究所 | Cordyceps cardinalis, method for preparing oosporin by using same and application of Cordyceps cardinalis |
| CN102168108B (en) * | 2010-12-17 | 2013-04-24 | 广东省微生物研究所 | Cordyceps cardinalis, method for preparing oosporin by using same and application of Cordyceps cardinalis |
| CN102266054B (en) * | 2011-07-26 | 2013-01-30 | 西藏月王生物技术有限公司 | Fermentation functionality aweto beverage and preparation method thereof |
| CN102266054A (en) * | 2011-07-26 | 2011-12-07 | 西藏月王生物技术有限公司 | Aweto ferment and preparation method thereof |
| CN102805401A (en) * | 2012-07-20 | 2012-12-05 | 黄晓青 | Chinese caterpillar fungus health-care drink prepared by liquid deep submerged fermentation and preparation method thereof |
| CN103355727A (en) * | 2013-08-06 | 2013-10-23 | 郑剑峰 | Preparation method for solid Cordyceps militaris beverage |
| CN103355727B (en) * | 2013-08-06 | 2014-10-29 | 郑剑峰 | Preparation method for solid Cordyceps militaris beverage |
| CN104473977A (en) * | 2014-11-18 | 2015-04-01 | 广东省微生物研究所 | Cordyceps militaris water extract as well as preparation method and application thereof |
| CN105661240A (en) * | 2016-03-10 | 2016-06-15 | 浙江博士园生物技术有限公司 | Cordyceps militaris beverage and making method thereof |
| CN108056307A (en) * | 2017-11-30 | 2018-05-22 | 安徽壮大饲料有限公司 | A kind of laying duck seedling feed and preparation method thereof |
| CN108576504A (en) * | 2018-03-29 | 2018-09-28 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of wax gourd high microsteping beverage |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101066459B1 (en) | An Enhanced Health Functional Schizandra extract Makgeolli and Process for Preparation thereof | |
| KR101034285B1 (en) | Takju containing sap and preparing method thereof | |
| CN101492638A (en) | Process for producing pure-juice full-fermentation watermelon wine | |
| CN108660039A (en) | The preparation process of matrimony vine alcoholic drink mixed with fruit juice and matrimony vine alcoholic drink mixed with fruit juice | |
| CN112877165A (en) | Fermented privet cider and preparation method thereof | |
| CN1075067A (en) | The preparation method of north cordyceps sinensis beverage | |
| CN112430515A (en) | Production method of low-sugar low-alcohol mulberry wine | |
| CN1772868A (en) | Prepn process of longan wine | |
| CN104498253B (en) | Brewing method of roselle honey wine | |
| CN106222011A (en) | The brewing method of Herba Dendrobii fructus momordicae wine | |
| CN113881539B (en) | Lycium barbarum juice wine and preparation method thereof | |
| CN1944619A (en) | Method for producing bamboo juice wine | |
| CN113337358A (en) | Preparation method of mulberry yellow wine | |
| CN1978626B (en) | Method for brewing papermulberry fruit health red wine | |
| CN110093239B (en) | Noni compound wine and preparation method thereof | |
| CN112195080A (en) | Production method of mulberry brandy | |
| CN108220070B (en) | High-chroma dry hawthorn wine and preparation method thereof | |
| KR100643735B1 (en) | Fermented Wine made from Fruit of Araliaceous Shrubs, and Method for producing Thereof | |
| CN110564575A (en) | Dendrobium officinale wine capable of protecting intestines and stomach and beautifying skin and preparation method thereof | |
| CN1091145C (en) | Preparation process and product of tasty and refreshing hill grape wine | |
| CN107557222A (en) | A kind of compound brandy brewage process | |
| CN1165609C (en) | Method for preparing health care herbal liquor | |
| CN107164186A (en) | A kind of okra health preserving wine and preparation method thereof | |
| CN1083099A (en) | Process for producing spirit of north Chinese cater pillar fungus | |
| CN113621464A (en) | Fermentation process of russianolive flower-flavored fruit wine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |