CN107505434A - anaerobic fermentation gas evaluation device and method - Google Patents

anaerobic fermentation gas evaluation device and method Download PDF

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Publication number
CN107505434A
CN107505434A CN201710851792.7A CN201710851792A CN107505434A CN 107505434 A CN107505434 A CN 107505434A CN 201710851792 A CN201710851792 A CN 201710851792A CN 107505434 A CN107505434 A CN 107505434A
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data
mcu
module
temperature
anaerobism
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蒲施桦
龙定彪
王浩
曾雅琼
李爽
刘作华
周婵
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Chongqing Academy of Animal Sciences
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Chongqing Academy of Animal Sciences
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0004Gaseous mixtures, e.g. polluted air
    • G01N33/0009General constructional details of gas analysers, e.g. portable test equipment
    • G01N33/0027General constructional details of gas analysers, e.g. portable test equipment concerning the detector
    • G01N33/0036General constructional details of gas analysers, e.g. portable test equipment concerning the detector specially adapted to detect a particular component
    • G01N33/0054Ammonia

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Combustion & Propulsion (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a kind of anaerobic fermentation gas evaluation device and method, belong to field of gas detection.The device includes anaerobic jar, data acquisition board, top cover, bleeding point, valve, aspiration pump, exhaust pipe, anaerobism end data module and PC end data modules;Anaerobic jar is used to place sample, and top is used for sealed sample provided with top cover;Data acquisition board is arranged inside of top cover, and for gathering the status data of sample, data acquisition board is provided with ammonia gas sensor, humiture and baroceptor and MCU;And sent by anaerobism end data module to PC end data modules.Gas probe is arranged on fermentation inside by the present invention, solves the problems, such as to influence anaerobic fermentation environmental condition in gas detection process, while continuous, the real-time detection gas concentration of energy, the change procedure of discharge capacity, it is ensured that the continuity and validity of experimental result.

Description

Anaerobic fermentation gas evaluation device and method
Technical field
The invention belongs to field of gas detection, is related to a kind of anaerobic fermentation gas evaluation device and method.
Background technology
Caused exhaust gas composition in the fermentation process of microorganism, represent the material situation of change in fermentation process.Especially It is the gas discharge characteristics such as caused ammonia, hydrogen sulfide in fermentation process, the composition regulation in agriculture excrement composting, anaerobism is sent out The technical combinations optimization of ferment and the efficient utilization and the depollution of environment of the fermentation energy have great importance.
The gas detection equipment of existing market, it is mainly used under the aerobic or micro- oxygen environment of open either semi open model Eudiometry, and the tail gas on-line checking research and development for anaerobic fermentation are fresh few, because anaerobic fermentation has necessarily for yeasting Requirement, therefore, design a kind of tail gas online detection instrument of suitable anaerobic fermentation, discharged for the gas of anaerobic fermentation process The research of feature has great importance.
The country mainly has for the assay method of anaerobic fermentation gas:Chu Lili etc., for research different ratio pig manure, wheat Stalk hybrid anaerobic fermentation term feature, it is autonomous be provided with can constant-temperature anaerobic fermentation device, by installation for fermenting, gas collecting apparatus and control The warm part of device 3 composition, eudiometry are collected using pumping, and supporting associated gas measure device is analyzed.
Liu Yi is isolated and purified with term effect disquisition in methane backeria, and methane backeria is divided in material anaerobic fermentation gas production measure In analysis, gas collection is carried out using hydrostatic(al) process, the measure that gas is carried out according to the chemical absorption method of national standard is analyzed.
The Exhaust measurement of present anaerobic fermentation, mainly by the way of being manually evacuated, the gas in fermentation is extracted out, carried out Chemical analysis determines, and this mode is insufficient in the presence of being evacuated, and gas gas production can lose during extraction, causes to determine number According to having differences property, simultaneously because air suction mode can influence the environmental index changes such as temperature in yeasting, humidity, destroy close Reaction environment condition is closed, so as to influence the operation of anaerobic fermentation later stage, therefore most of fermentation gas determination experiment needs setting more Individual parallel reaction sample, for the detection of stage, so not only experimental work amount is big, and the individual difference between each sample Property is big, and data analysis difficulty is big.
At present, the not no detection device on anaerobic fermentation tail gas, existing anaerobic fermentation tail gas technology, still using artificial The mode of pumping, experiments supporting room chemical analysis or gas apparatus detection are carried out, and this mode can influence the ring of fermenting experiment Border condition, it is unfavorable for the tail gas observation detection of chronicity fermentation.
The content of the invention
In view of this, it is an object of the invention to provide a kind of anaerobic fermentation gas evaluation device, anaerobism can strictly be controlled The ambient parameter of fermentation, while anaerobic fermentation environmental condition can not be influenceed, gas in real-time continuous detection anaerobic fermentation process Concentration, discharge capacity, gas detection are sensed using sensing probe, and detection accuracy reaches 0.1ppm, can precisely sensitive measure fermentation Middle minimum gas produces, while supporting online gas identification, data processing, is provided for anaerobic bacteria or fermenting experiment metabolic analysis Detailed gas data analysis result, reduces artificial calculation error.
To reach above-mentioned purpose, the present invention provides following technical scheme:
Anaerobic fermentation gas evaluation device, the device include anaerobic jar (1), data acquisition board (2), top cover (3), anaerobism end Data module (8) and PC end datas module (9);
The anaerobic jar (1) is used to place sample, and top is used for sealed sample provided with top cover (3);The data acquisition board (2) it is arranged on the inside of top cover (3), is sent for gathering the status data of sample, and by anaerobism end data module (8) to PC ends Data module (9).
Further, described device also includes bleeding point (4), valve (5), aspiration pump (6) and exhaust pipe (7);
The bleeding point (4) is arranged on top cover (3), is connected by exhaust pipe (7) with aspiration pump (6), the pumping Pump (6) is used for the negative pressure for extracting anaerobic jar (1) interior certain value, and the exhaust pipe (7) is provided with valve (5), controls exhaust tube The opening and closing on road (7).
Further, the data acquisition board (2) is provided with ammonia gas sensor, humiture and baroceptor and micro-control Unit (Microcontroller Unit, MCU) processed;
The data receiver pin RXD2 and MCU of the ammonia gas sensor data receiver pin RXD2 are connected, ammonia sensing The data of device send pin TXD2 and are connected with MCU Data Data transmission pin TXD2, while ammonia gas sensor is grounded;
The data wire SDA and MCU of the humiture and baroceptor data wire SDA are connected, humiture and air pressure transmission The clock cable SCL and MCU of sensor clock cable SCL are connected, while humiture and baroceptor ground connection;
Meanwhile the MCU connects power supply and ground connection.
Further, the anaerobism end data module (8) is in data acquisition board (2), the anaerobism end data module (8) It is wireless transport module or wire transmission module with PC end datas module (9);
The data receiver pin RXD1 and MCU of the anaerobism end data module (8) data receiver pin RXD1 are connected, and detest The data of oxygen end data module (8) send pin TXD1 and are connected with MCU Data Data transmission pin TXD1, while anaerobism end number It is grounded according to module (8).
Anaerobic fermentation gas evaluation method based on described device, comprises the following steps:
S1:MCU is initialized, obtains compensation coefficient inside humiture and baroceptor, and preheat;
S2:Whether detection anaerobism end data module (8) receives PC end datas module (9) instruction;If receiving, anaerobism End data module (8) obtains data and sends interval and preserve, and enters step S3;If being not received by, step is directly entered S3;
S3:MCU detects whether the new data of ammonia gas sensor, if so, then MCU parses the new data of ammonia gas sensor, Ammonia concentration is obtained, and is sampled by humiture and baroceptor, obtains temperature, pressure, the value of humidity, and combines correction system Number fits the analogue value, subsequently into step S4;If nothing:Then it is directly entered step S4;
S4:MCU detection data send whether interval reaches;If reaching, anaerobism end data module (8) is called to send sampling Data afterwards, into step S2;If not reaching, step S2 is directly entered.
Further, in the step S3, humiture and baroceptor obtain sound pressure sensitivity C1, expression formula SENST1; Pressure compensation C2, expression formula OFFT1;Presser sensor temperature coefficient C3 expression formulas are TCS;Pressure compensation temperature coefficient C4, expression Formula is TCO, reference temperature C5, expression formula TREF;The temperature coefficient C6 of temperature, expression formula TEMPSENS;Pressure value digital quantity D1, temperature value digital quantity D2;Humidity D3;
1) temperature is specially:
The difference dT=D2-T of actual temperature and reference temperatureREF=D2-C5*28,
Actual temperature (- 40~85 DEG C of resolution ratio are 0.01) TEMP=20+dT*TEMPSENS=2000+dT*C6/223
2) pressure is specially:
Actual temperature offsets OFF=OFFT1+ TCS*dT=C2*216+(C3*dT)/27,
Actual temperature sensitivity S ENS=SENST1+ TCS*dT=C1*216+(C3*dT)/27,
Temperature compensated pressure (10~1200mbar resolution ratio is 0.01mbar),
P-D1*SENS-OFF=(D1*SENS/221-OFF)/215
3) humidity is specially:
Actual relative humidity (- 6%RH~118% resolution ratio be RH0.01%RH) RH=100* (- 6+125*D3/216)。
The beneficial effects of the present invention are:Gas probe is arranged on fermentation inside by the present invention, is solved gas and was detected The problem of anaerobic fermentation environmental condition is influenceed in journey, while continuous, the real-time detection gas concentration of energy, the change procedure of discharge capacity, Ensure the continuity and validity of experimental result.Environmental control system and the gas detection point of the anaerobic fermentation conditions of coordinative composition of equipments Analysis system, while strictly control anaerobic fermentation environment, gas detection data is analyzed and processed in time, have effectively evaded artificial behaviour The resultant error of work.
Brief description of the drawings
In order that the purpose of the present invention, technical scheme and beneficial effect are clearer, the present invention provides drawings described below and carried out Explanation:
Fig. 1 is schematic structural view of the invention;
Fig. 2 is apparatus of the present invention circuit total figure;
Fig. 3 is MCU circuit diagrams.
Embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described in detail.
As shown in figure 1, anaerobic fermentation gas evaluation device, including anaerobic jar 1, data acquisition board 2, top cover 3, bleeding point 4, Valve 5, aspiration pump 6, exhaust pipe 7, anaerobism end data module 8 and PC end datas module 9;
Anaerobic jar 1 is used to place sample, and top is used for sealed sample provided with top cover 3;Data acquisition board 2 is arranged at top cover 3 Inner side, sent for gathering the status data of sample, and by anaerobism end data module 8 to PC end datas module 9.
Bleeding point 4 is arranged on top cover 3, is connected by exhaust pipe 7 with aspiration pump 6, and aspiration pump 6 is used to extract anaerobic jar The negative pressure of certain value in 1, exhaust pipe 7 are provided with valve 5, control the opening and closing of exhaust pipe 7.
Anaerobism end data module 8 and PC end datas module 9 are wireless transport module;Anaerobism end data module 8 be monolithic without Line transceiver chip NRF24L01, in data acquisition board 2;
As shown in Fig. 2 data acquisition board be provided with ammonia gas sensor, humiture and baroceptor (TPH sensors), with And micro-control unit (MicrocontrollerUnit, MCU);
The data receiver pin RXD2 and MCU of ammonia gas sensor data receiver pin RXD2 are connected, ammonia gas sensor Data send pin TXD2 and are connected with MCU Data Data transmission pin TXD2, while ammonia gas sensor is grounded;
The data wire SDA and MCU of humiture and baroceptor data wire SDA are connected, humiture and baroceptor Clock cable SCL be connected with MCU clock cable SCL, while humiture and baroceptor ground connection;
Meanwhile MCU connects power supply and ground connection.
The data receiver pin RXD1 and MCU of monolithic wireless transceiver chip data receiver pin RXD1 are connected, monolithic The data of wireless transceiver chip send pin TXD1 and are connected with MCU Data Data transmission pin TXD1, while monolithic is wireless Transponder chip is grounded.
Fig. 3 is MCU circuit diagrams, the anaerobic fermentation gas evaluation method based on described device, is comprised the following steps:
S1:MCU is initialized, obtains compensation coefficient inside humiture and baroceptor, and preheat;
S2:Whether detection anaerobism end data module 8, which receives PC end datas module 9, instructs;If receiving, anaerobism end number Data transmission interval is obtained according to module 8 and is preserved, and enters step S3;If being not received by, step S3 is directly entered;
S3:MCU detects whether the new data of ammonia gas sensor, if so, then MCU parses the new data of ammonia gas sensor, Ammonia concentration is obtained, and is sampled by humiture and baroceptor, obtains temperature, pressure, the value of humidity, and combines correction system Number fits the analogue value, subsequently into step S4;If nothing:Then it is directly entered step S4;
Humiture and baroceptor obtain sound pressure sensitivity C1, expression formula SENST1;Pressure compensation C2, expression formula are OFFT1;Presser sensor temperature coefficient C3 expression formulas are TCS;Pressure compensation temperature coefficient C4, expression formula TCO, reference temperature C5, Expression formula is TREF;The temperature coefficient C6 of temperature, expression formula TEMPSENS;It is as shown in the table:
Humiture atmospheric pressure sensor corrects value list
Variable Type Expression formula
C1 Sound pressure sensitivity SENST1
C2 Pressure compensation OFFT1
C3 Presser sensor temperature coefficient TCS
C4 Pressure compensation temperature coefficient TCO
C5 Reference temperature TREF
C6 The temperature coefficient of temperature TEMPSENS
Obtain pressure value digital quantity D1, temperature value digital quantity D2;It is as shown in the table:
D1 Pressure value digital quantity
D2 Temperature value digital quantity
D3 Humidity
1) temperature is specially:
The difference dT=D2-T of actual temperature and reference temperatureREF=D2-C5*28,
Actual temperature (- 40~85 DEG C of resolution ratio are 0.01) TEMP=20+dT*TEMPSENS=2000+dT*C6/223
2) combination temperature is corrected, and pressure is specially:
Actual temperature offsets OFF=OFFT1+ TCS*dT=C2*216+(C3*dT)/27,
Actual temperature sensitivity S ENS=SENST1+ TCS*dT=C1*216+(C3*dT)/27,
Temperature compensated pressure (10~1200mbar resolution ratio is 0.01mbar),
P-D1*SENS-OFF=(D1*SENS/221-OFF)/215
3) humidity is specially:
RH Actual relative humidity (- 6%RH~118% resolution ratio be RH0.01%RH) RH=100* (- 6+125*D3/216)
Actual relative humidity (- 6%RH~118% resolution ratio be RH0.01%RH) RH=100* (- 6+125*D3/216)。
S4:MCU detection data send whether interval reaches;If reaching, after calling anaerobism end data module 8 to send sampling Data, into step S2;If not reaching, step S2 is directly entered.
Finally illustrate, preferred embodiment above is merely illustrative of the technical solution of the present invention and unrestricted, although logical Cross above preferred embodiment the present invention is described in detail, it is to be understood by those skilled in the art that can be Various changes are made to it in form and in details, without departing from claims of the present invention limited range.

Claims (6)

1. anaerobic fermentation gas evaluation device, it is characterised in that:The device includes anaerobic jar (1), data acquisition board (2), top cover (3), anaerobism end data module (8) and PC end datas module (9);
The anaerobic jar (1) is used to place sample, and top is used for sealed sample provided with top cover (3);The data acquisition board (2) sets It is placed on the inside of top cover (3), is sent for gathering the status data of sample, and by anaerobism end data module (8) to PC end datas Module (9).
2. anaerobic fermentation gas evaluation device according to claim 1, it is characterised in that:Described device also includes bleeding point (4), valve (5), aspiration pump (6) and exhaust pipe (7);
The bleeding point (4) is arranged on top cover (3), is connected by exhaust pipe (7) with aspiration pump (6), the aspiration pump (6) For extracting the negative pressure of anaerobic jar (1) interior certain value, the exhaust pipe (7) is provided with valve (5), control exhaust pipe (7) Opening and closing.
3. anaerobic fermentation gas evaluation device according to claim 1 or 2, it is characterised in that:The data acquisition board (2) It is provided with ammonia gas sensor, humiture and baroceptor and micro-control unit (Microcontroller Unit, MCU);
The data receiver pin RXD2 and MCU of the ammonia gas sensor data receiver pin RXD2 are connected, ammonia gas sensor Data send pin TXD2 and are connected with MCU Data Data transmission pin TXD2, while ammonia gas sensor is grounded;
The data wire SDA and MCU of the humiture and baroceptor data wire SDA are connected, humiture and baroceptor Clock cable SCL be connected with MCU clock cable SCL, while humiture and baroceptor ground connection;
Meanwhile the MCU connects power supply and ground connection.
4. anaerobic fermentation gas evaluation device according to claim 3, it is characterised in that:The anaerobism end data module (8) in the data acquisition board (2), the anaerobism end data module (8) and PC end datas module (9) be wireless transport module or Wire transmission module;
The data receiver pin RXD1 and MCU of the anaerobism end data module (8) data receiver pin RXD1 are connected, anaerobism end The data of data module (8) send pin TXD1 and are connected with MCU Data Data transmission pin TXD1, while anaerobism end data mould Block (8) is grounded.
5. the anaerobic fermentation gas evaluation method based on the described device of claim 3 or 4, it is characterised in that:This method include with Lower step:
S1:MCU is initialized, obtains compensation coefficient inside humiture and baroceptor, and preheat;
S2:Whether detection anaerobism end data module (8) receives PC end datas module (9) instruction;If receiving, anaerobism end number Data transmission interval is obtained according to module (8) and is preserved, and enters step S3;If being not received by, step S3 is directly entered;
S3:MCU detects whether the new data of ammonia gas sensor, if so, then MCU parses the new data of ammonia gas sensor, obtains Ammonia concentration, and sampled by humiture and baroceptor, temperature, pressure, the value of humidity are obtained, and combine compensation coefficient and intend The analogue value is closed out, subsequently into step S4;If nothing:Then it is directly entered step S4;
S4:MCU detection data send whether interval reaches;If reaching, after calling anaerobism end data module (8) to send sampling Data, into step S2;If not reaching, step S2 is directly entered.
6. anaerobic fermentation gas evaluation method according to claim 5, it is characterised in that:In the step S3, humiture Sound pressure sensitivity C1, expression formula SENS are obtained with baroceptorT1;Pressure compensation C2, expression formula OFFT1;Presser sensor Temperature coefficient C3 expression formulas are TCS;Pressure compensation temperature coefficient C4, expression formula TCO, reference temperature C5, expression formula TREF; The temperature coefficient C6 of temperature, expression formula TEMPSENS;Pressure value digital quantity D1, temperature value digital quantity D2;Humidity D3;
1) temperature is specially:
The difference dT=D2-T of actual temperature and reference temperatureREF=D2-C5*28,
- 40~85 DEG C of resolution ratio are 0.01, actual temperature TEMP=20+dT*TEMPSENS=2000+dT*C6/223
2) pressure is specially:
Actual temperature offsets OFF=OFFT1+ TCS*dT=C2*216+(C3*dT)/27,
Actual temperature sensitivity S ENS=SENST1+ TCS*dT=C1*216+(C3*dT)/27,
Temperature compensated pressure, 10~1200mbar resolution ratio are 0.01mbar,
P-D1*SENS-OFF=(D1*SENS/221-OFF)/215
3) humidity is specially:
The resolution ratio of -6%RH~118% is RH0.01%RH, actual relative humidity RH=100* (- 6+125*D3/216)。
CN201710851792.7A 2017-09-19 2017-09-19 anaerobic fermentation gas evaluation device and method Pending CN107505434A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111454819A (en) * 2020-04-14 2020-07-28 坤德盛科(北京)科技有限公司 Organic membrane fermentation device and use method thereof
CN116577128A (en) * 2023-07-14 2023-08-11 合肥工业大学 Intelligent collection and analysis method for biodegradable plastic wastewater treatment data

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102353798A (en) * 2011-07-01 2012-02-15 华东理工大学 Biological fermentation process-oriented olfactory analog instrument and online analysis method
CN105585129A (en) * 2016-01-12 2016-05-18 南京大学 Device and method for simulating fate of nitrogen in in-situ river channel ecosystem
CN206074386U (en) * 2016-08-25 2017-04-05 中国农业科学院农产品加工研究所 Determine the device of dough fermentability
CN207215791U (en) * 2017-09-19 2018-04-10 重庆市畜牧科学院 anaerobic fermentation gas evaluation device

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102353798A (en) * 2011-07-01 2012-02-15 华东理工大学 Biological fermentation process-oriented olfactory analog instrument and online analysis method
CN105585129A (en) * 2016-01-12 2016-05-18 南京大学 Device and method for simulating fate of nitrogen in in-situ river channel ecosystem
CN206074386U (en) * 2016-08-25 2017-04-05 中国农业科学院农产品加工研究所 Determine the device of dough fermentability
CN207215791U (en) * 2017-09-19 2018-04-10 重庆市畜牧科学院 anaerobic fermentation gas evaluation device

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111454819A (en) * 2020-04-14 2020-07-28 坤德盛科(北京)科技有限公司 Organic membrane fermentation device and use method thereof
CN116577128A (en) * 2023-07-14 2023-08-11 合肥工业大学 Intelligent collection and analysis method for biodegradable plastic wastewater treatment data
CN116577128B (en) * 2023-07-14 2023-09-15 合肥工业大学 Intelligent collection and analysis method for biodegradable plastic wastewater treatment data

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