CN107496992B - Three-dimensional patterned Fe3O4Medical polymer material composite nanofiber and preparation method thereof - Google Patents
Three-dimensional patterned Fe3O4Medical polymer material composite nanofiber and preparation method thereof Download PDFInfo
- Publication number
- CN107496992B CN107496992B CN201710766396.4A CN201710766396A CN107496992B CN 107496992 B CN107496992 B CN 107496992B CN 201710766396 A CN201710766396 A CN 201710766396A CN 107496992 B CN107496992 B CN 107496992B
- Authority
- CN
- China
- Prior art keywords
- composite nanofiber
- polymer material
- dimensional
- nanofiber
- patterned
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/025—Other specific inorganic materials not covered by A61L27/04 - A61L27/12
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F1/00—General methods for the manufacture of artificial filaments or the like
- D01F1/02—Addition of substances to the spinning solution or to the melt
- D01F1/10—Other agents for modifying properties
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
- D01F6/00—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof
- D01F6/88—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof from mixtures of polycondensation products as major constituent with other polymers or low-molecular-weight compounds
- D01F6/92—Monocomponent artificial filaments or the like of synthetic polymers; Manufacture thereof from mixtures of polycondensation products as major constituent with other polymers or low-molecular-weight compounds of polyesters
Abstract
The invention discloses three-dimensional patterned Fe3O4Medical polymer material composite nanofiber and a preparation method thereof. The method comprises the steps of mixing Fe3O4Ultrasonically mixing the nano particles with a solvent, and adding a medical high polymer material to obtain an electrospinning solution; starting electrospinning by using the electrospinning solution to obtain the composite nanofiber; separating from a receiving device, drying in the air, cutting into small pieces, picking and wetting the small pieces by tweezers, and placing the small pieces into a culture dish with the bottom preset with rubidium, iron and boron permanent magnets; stacking the obtained small pieces in a culture dish by continuous tweezers to obtain the three-dimensional patterned Fe3O4Medical polymer material composite nanofiber. The three-dimensional patterned composite nanofiber provided by the invention can not only not damage the original microscopic bionic structure of the electrostatic spinning nanofiber, but also break the two-dimensional plane limitation of the electrostatic spinning technology applied to a tissue engineering scaffold, and provides a non-contact efficient bionic three-dimensional construction method, and the patterned porous structure is beneficial to cell adhesion, proliferation and the like.
Description
Technical Field
The invention relates to the field of three-dimensional nanofibers of multi-stage composite through pore structures, in particular to three-dimensional patterned Fe3O4Medical polymer material composite nanofiber and a preparation method thereof.
Background
In recent years, the continuous development of tissue engineering provides powerful guarantee for human health, wherein the scaffold material has been a road for bionic construction for decades as a ring for gathering human intelligence most in tissue engineering. Among various techniques for preparing scaffold materials, the electrospinning technique has wide application in the field of tissue engineering because the electrospinning technique can prepare nanofibers of a microstructure bionic extracellular matrix. However, the electrospinning technique has limited application due to the difficulty in constructing three-dimensional nanofiber materials.
Three-dimensional nanofiber materials cannot be obtained by simply increasing the sample preparation time, only the porosity of the sample is reduced, and over time, the coverage of the receiver by the fibers leads to a reduction in conductivity and thus reception efficiency, and ultimately to spinning failure. The current methods for preparing three-dimensional structures by electrospinning techniques can be broadly classified into the following four types. (1) Continuous electrospinning or multilayer electrospinning. Preparing thin-layer fibers in a mode similar to two-dimensional plane electrospinning, and then carrying out continuous electrospinning through parameter adjustment. Three-dimensional porous structures with a thickness of several hundred micrometers can be produced using this method. (2) The three-dimensional structure is built up by post-treatment of the prepared fibre material, for example folding, crimping or layer-by-layer stacking. (3) The nanofibers are prepared by designing a receptor having a three-dimensional structure as a template instead of a two-dimensional planar receptor to obtain a three-dimensional structure. (4) Rapid stacking or assembly of silk fibers to obtain a three-dimensional structure is achieved by varying parameters of electrospinning, such as electric field strength, solution concentration, and relative humidity.
But on the one hand, the use of the methods can cause relatively low efficiency due to great operation difficulty; on the other hand, the original microstructure bionic characteristics of the electrostatic spinning nanofiber can be destroyed to a certain extent in the treatment process, and a macroscopic-microscopic multistage composite through pore structure cannot be formed, so that a required three-dimensional microenvironment cannot be provided for cells. Therefore, how to effectively prepare the three-dimensional nanofiber with the multilevel composite through pore structure by utilizing the electrospinning technology is a problem to be solved urgently at present.
Disclosure of Invention
The invention aims to overcome the defect that three-dimensional nanofibers cannot be quickly and effectively constructed by electrospinning at present, and provides a three-dimensional patternDissolving Fe3O4The method is based on an electrostatic spinning technology, and can efficiently and quickly prepare the three-dimensional scaffold with the multi-stage composite through pore structure under the condition of not introducing other complex technologies and post-treatment, namely, the electro-spinning composite magnetic nanofiber, and can realize rapid patterning and three-dimensional construction under the action of a magnetic field.
The purpose of the invention is realized by the following technical scheme.
Three-dimensional patterned Fe3O4The preparation method of the medical polymer material composite nanofiber comprises the following steps:
(1) mixing Fe3O4The nano particles and the solvent are ultrasonically mixed to obtain uniformly dispersed Fe3O4Suspending liquid;
(2) adding Fe obtained in step (1)3O4Adding medical high polymer materials into the suspension, and uniformly mixing to obtain an electrospinning solution;
(3) using the stainless steel grid as a receiving device, and starting electrospinning by using the electrospinning liquid in the step (2) to obtain patterned Fe3O4Medical polymer material composite nanofiber;
(4) patterning Fe obtained in the step (3)3O4Separating the medical polymer material composite nanofiber from a receiving device, airing, cutting into composite nanofiber chips, then tweezing and wetting the composite nanofiber chips, and putting the composite nanofiber chips into a culture dish with a rubidium iron boron permanent magnet preset at the bottom;
(5) stacking the obtained composite nanofiber chips in the culture dish in the step (4) by continuous tweezers to obtain the three-dimensional patterned Fe with the multi-stage composite through pore structure3O4Medical polymer material composite nanofiber.
Preferably, the solvent in step (1) is dichloromethane and N, N-dimethylformamide.
Preferably, the ultrasound in step (1) is performed twice, each time for 30min, and the heated water in the ultrasound pool is replaced by cold water during the ultrasound process.
Preferably, the polymer material in the step (2) is a medical polymer material such as Polycaprolactone (PCT).
Preferably, the electrospinning conditions in step (3) are as follows: the feeding speed is 1 ml/h, the rotating speed of the rotating shaft is 120 +/-10 r/min, the receiving height is 13 +/-2 cm, the temperature of the working bin is 43 +/-2 ℃, and the relative humidity is 40 +/-5%.
Preferably, the relative positions of the upper layer and the lower layer are adjusted to ensure that the grids correspond to each other when the composite nanofiber platelets are stacked in the step (5).
Three-dimensional patterned Fe with multi-level composite through pore structure prepared by the preparation method3O4Medical polymer material composite nanofiber.
Based on the electrostatic spinning technology, firstly, the composite nano-fiber grid material doped with the magnetic iron oxide nano-particles is rapidly prepared by taking a stainless steel grid as a template receiving device. Then inoculating the cells on the surface of the material, accumulating the lower layer under the action of an external magnetic field to quickly construct a three-dimensional structure, culturing in vitro for a certain time, and removing the magnetic field to form a three-dimensional cell-material complex which is bonded by extracellular matrix as a whole.
Compared with the prior art, the invention has the following advantages:
the invention firstly uses the stainless steel grid as a receiving device to carry out patterning treatment on the magnetic composite nanofiber, and can quickly obtain the composite nanofiber with a central through hole structure; then, the patterned composite nano-fiber is stacked, and the three-dimensional Fe with the macroscopic-microscopic composite through pore structure can be quickly constructed3O4Medical polymer material composite nanofiber. The invention provides efficient non-contact three-dimensional construction, is simple and convenient to operate, and does not destroy the original microstructure bionic characteristics of the electrostatic spinning nanofiber. In addition, the multilevel composite through pore structure of the composite nanofiber is more beneficial to cell behaviors such as adhesion, proliferation and the like of various seed cells in tissue engineering.
Drawings
FIGS. 1a and 1b show the patterned Fe obtained in example 13O4An electron microscope image of the microscopic morphology of the medical polymer composite nanofiber;
FIGS. 2a and 2b show the patterned Fe obtained in example 23O4An electron microscope image of the microscopic morphology of the medical polymer composite nanofiber;
FIG. 3 is the three-dimensional patterned Fe obtained in example 23O4A macroscopic view of the medical polymer material composite nanofiber;
FIGS. 4a and 4b show three-dimensional patterns of Fe obtained in example 23O4/electron microscope image of medical polymer composite nanofiber.
FIG. 5 shows three-dimensional patterned Fe obtained in examples 1 and 23O4Proliferation data diagram of medical polymer material composite nanofiber.
FIG. 6a is an adhesion electron microscope image of the medical polymer material polycaprolactone nanofiber.
FIG. 6b is the three-dimensional patterned Fe obtained in example 13O4Adhesion electron microscope picture of medical polymer material composite nanofiber.
FIG. 6c shows three-dimensionally patterned Fe obtained in example 23O4Adhesion electron microscope picture of medical polymer material composite nanofiber.
Detailed Description
Specific embodiments of the present invention will be further described below with reference to the following examples and drawings, but the present invention is not limited thereto.
Example 1
0.42g of Fe was weighed3O4Placing the nano particles in a penicillin bottle, wherein the volume ratio of the nano particles to the penicillin bottle is 1: 1, respectively measuring 3ml of dichloromethane and 3ml of N, N-dimethylformamide, adding into a penicillin bottle, transferring into an ultrasonic cleaning machine for ultrasonic dispersion twice for 30 min/time, and replacing heated water in the ultrasonic cleaning machine with cold water in the ultrasonic process. Then weighing 0.84g of polycaprolactone, adding the polycaprolactone into the penicillin bottle, quickly transferring the polycaprolactone to a high-speed (1500 rpm) oscillation blending instrument, and carrying out oscillation blending for 24 hours to obtain Fe3O4Medical polymer material electrospinning liquid. Using a hand-held small electric cutter as a receiverCutting a 304 stainless steel grid of the device into 25cm multiplied by 8cm grids, ultrasonically cleaning the grids by using an ultrasonic cleaning machine, and airing the grids for later use; electrospinning was started under the following conditions: the feeding speed is 1 ml/h, the rotating speed of the rotating shaft is 120 r/min, the receiving height is 13 cm, the temperature of the working chamber is 43 +/-2 ℃, and the relative humidity is 40% +/-5, so that the patterned Fe is obtained3O4Medical polymer material composite nanofiber, as shown in figure 1a and figure 1b, Fe3O4Uniformly dispersed in the polycaprolactone fiber.
Patterning the prepared Fe3O4The medical polymer material composite nano fiber is separated from the receiver and dried, and then is divided into small pieces of 1cm multiplied by 1 cm. Using tweezers to pick the wetted nanofiber chips and placing the nanofiber chips into a culture dish with the bottom preset with rubidium iron boron permanent magnets, continuously picking the magnetic fiber chips by tweezers and adjusting the relative positions of the upper layer and the lower layer to ensure that grids correspond to each other, and further quickly constructing the three-dimensional patterned Fe with the macroscopic-microscopic composite through pore structure3O4Medical polymer material composite nanofiber. The multilevel composite through pore structure of the composite nanofiber is more beneficial to cell behaviors such as adhesion, proliferation and the like of various seed cells in tissue engineering.
Example 2
0.63g of Fe was weighed3O4Placing the nano particles in a penicillin bottle, wherein the volume ratio of the nano particles to the penicillin bottle is 1: 1, respectively measuring 3ml of dichloromethane and 3ml of N, N-dimethylformamide, adding into a penicillin bottle, transferring into an ultrasonic cleaning machine for ultrasonic dispersion twice for 30 min/time, and replacing heated water in the ultrasonic cleaning machine with cold water in the ultrasonic process. Then weighing 0.84g of polycaprolactone, adding the polycaprolactone into the penicillin bottle, quickly transferring the polycaprolactone to a high-speed (1500 rpm) oscillation blending instrument, and carrying out oscillation blending for 24 hours to obtain Fe3O4Medical polymer material electrospinning liquid. Cutting a 304 stainless steel grid serving as a receiver into 25cm multiplied by 8cm grids by using a handheld small electric cutting machine, ultrasonically cleaning by using an ultrasonic cleaning machine, and airing for later use; electrospinning was started under the following conditions: the feeding speed is 1 ml/h, the rotating speed of the rotating shaft is 130 r/min, the receiving height is 15 cm, the temperature of the working chamber is 43 +/-2 ℃, and the relative humidity is 40% +/-5, so that the pattern is obtainedTransformed Fe3O4Medical polymer material composite nanofiber, as shown in fig. 2a and 2b, Fe3O4The polycaprolactone fiber is uniformly dispersed in the polycaprolactone fiber, the fiber surface is slightly rough, the material has an obvious grid-shaped structure as can be seen from figures 4a and 4b, and the silk fiber of the mesh part is sparse, so that the three-dimensional through hole is directly constructed after the three-dimensional through hole is superposed.
Patterning the prepared Fe3O4The medical polymer material composite nano fiber is separated from the receiver and dried, and then is divided into small pieces of 1cm multiplied by 1 cm. Using tweezers to pick the wetted nanofiber chips and placing the nanofiber chips into a culture dish with the bottom preset with rubidium iron boron permanent magnets, continuously picking the magnetic fiber chips by tweezers and adjusting the relative positions of the upper layer and the lower layer to ensure that grids correspond to each other, and further quickly constructing the three-dimensional patterned Fe with the macroscopic-microscopic composite through pore structure3O4The medical polymer material composite nanofiber has uniform and interconnected pores as shown in figure 3. The multilevel composite through pore structure of the composite nanofiber is more beneficial to cell behaviors such as adhesion, proliferation and the like of various seed cells in tissue engineering, and as can be seen in fig. 5, fig. 6a, fig. 6b and fig. 6c, the addition of the magnetic iron oxide nanoparticles enhances the surface biocompatibility of the material, so that the material is more suitable for the spreading of cells.
Claims (5)
1. Three-dimensional patterned Fe3O4The preparation method of the medical polymer material composite nanofiber is characterized by comprising the following steps:
(1) mixing Fe3O4The nano particles are ultrasonically mixed with a solvent to obtain uniformly dispersed Fe3O4Suspending liquid;
(2) adding Fe obtained in step (1)3O4Adding medical high polymer materials into the suspension, and uniformly mixing to obtain an electrospinning solution;
(3) using the stainless steel grid as a receiving device, and starting electrospinning by using the electrospinning liquid in the step (2) to obtain patterned Fe3O4Medical polymer material composite nanofiber;
(4) patterning Fe obtained in the step (3)3O4Separating the medical polymer material composite nanofiber from a receiving device, airing, cutting into composite nanofiber chips, then tweezing and wetting the composite nanofiber chips, and putting the composite nanofiber chips into a culture dish with a rubidium iron boron permanent magnet preset at the bottom;
(5) stacking the obtained composite nanofiber chips in the culture dish in the step (4) by continuous tweezers to obtain the three-dimensional patterned Fe with the multi-stage composite through pore structure3O4Medical polymer material composite nanofiber;
the solvent in the step (1) is dichloromethane and N, N-dimethylformamide;
and (3) the high polymer material in the step (2) is polycaprolactone.
2. The method of claim 1, wherein: the ultrasound in the step (1) is carried out twice, each time is 30min, and the heated water in the ultrasound pool is replaced by cold water in the ultrasound process.
3. The method of claim 1, wherein: the electrospinning conditions in the step (3) are as follows: the feeding speed is 1 ml/h, the rotating speed of the rotating shaft is 120 +/-10 r/min, the receiving height is 13 +/-2 cm, the temperature of the working bin is 43 +/-2 ℃, and the relative humidity is 40 +/-5%.
4. The method of claim 1, wherein: in the step (5), the relative positions of the upper layer and the lower layer are required to be adjusted to ensure that the grids correspond to each other when the composite nanofiber small pieces are stacked.
5. A three-dimensionally patterned Fe produced by the production method described in any one of claims 1 to 43O4Medical polymer material composite nanofiber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710766396.4A CN107496992B (en) | 2017-08-30 | 2017-08-30 | Three-dimensional patterned Fe3O4Medical polymer material composite nanofiber and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710766396.4A CN107496992B (en) | 2017-08-30 | 2017-08-30 | Three-dimensional patterned Fe3O4Medical polymer material composite nanofiber and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107496992A CN107496992A (en) | 2017-12-22 |
| CN107496992B true CN107496992B (en) | 2020-05-22 |
Family
ID=60693280
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710766396.4A Active CN107496992B (en) | 2017-08-30 | 2017-08-30 | Three-dimensional patterned Fe3O4Medical polymer material composite nanofiber and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107496992B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110004504A (en) * | 2019-05-24 | 2019-07-12 | 北京化工大学 | A kind of patterning electrostatic spinning apparatus |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1090928A1 (en) * | 1999-09-30 | 2001-04-11 | IsoTis N.V. | Polymers loaded with bioactive agents |
| CN102978151A (en) * | 2012-11-06 | 2013-03-20 | 中国科学院大连化学物理研究所 | Method for flexibly producing cell module of different morphology and application |
| CN103405809A (en) * | 2013-07-23 | 2013-11-27 | 东华大学 | Method used for preparing microcarrier/polymer composite scaffold by electro-deposition |
| CN104888278A (en) * | 2015-05-20 | 2015-09-09 | 东华大学 | Nanometer/micrometer fiber three-dimensional porous structure support material and preparation and application of support material |
| CN104963101A (en) * | 2015-06-12 | 2015-10-07 | 湖北立天生物工程有限公司 | Light and thin composite non-woven fabric high in performance and producing method thereof |
| WO2017079328A1 (en) * | 2015-11-02 | 2017-05-11 | Nanofiber Solutions, Inc. | Electrospun fibers having contrast agents and methods of making the same |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7790135B2 (en) * | 2003-07-02 | 2010-09-07 | Physical Sciences, Inc. | Carbon and electrospun nanostructures |
-
2017
- 2017-08-30 CN CN201710766396.4A patent/CN107496992B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1090928A1 (en) * | 1999-09-30 | 2001-04-11 | IsoTis N.V. | Polymers loaded with bioactive agents |
| CN102978151A (en) * | 2012-11-06 | 2013-03-20 | 中国科学院大连化学物理研究所 | Method for flexibly producing cell module of different morphology and application |
| CN103405809A (en) * | 2013-07-23 | 2013-11-27 | 东华大学 | Method used for preparing microcarrier/polymer composite scaffold by electro-deposition |
| CN104888278A (en) * | 2015-05-20 | 2015-09-09 | 东华大学 | Nanometer/micrometer fiber three-dimensional porous structure support material and preparation and application of support material |
| CN104963101A (en) * | 2015-06-12 | 2015-10-07 | 湖北立天生物工程有限公司 | Light and thin composite non-woven fabric high in performance and producing method thereof |
| WO2017079328A1 (en) * | 2015-11-02 | 2017-05-11 | Nanofiber Solutions, Inc. | Electrospun fibers having contrast agents and methods of making the same |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107496992A (en) | 2017-12-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105107022B (en) | A kind of preparation method of the nanofiber porous support with the elasticity of compression under hygrometric state | |
| Abel et al. | Combination of electrospinning with other techniques for the fabrication of 3D polymeric and composite nanofibrous scaffolds with improved cellular interactions | |
| CN106267339B (en) | A kind of super hydrophilic biological support preparation method of high-modulus | |
| CN101401955A (en) | Method for producing nano-fibre bracket material with levorotation polylactic acid as base material | |
| CN103820943B (en) | Macropore three-dimensional order orientation silk fibroin nano-fiber support and preparation method thereof | |
| CN103110979B (en) | High molecular porous material with surface deposited bone-like hydroxyapatite as well as preparation method and application thereof | |
| CN105926162A (en) | Method for preparing nanofibers adopting porous structure through electrostatic spinning | |
| Sheikh et al. | A simple approach for synthesis, characterization and bioactivity of bovine bones to fabricate the polyurethane nanofiber containing hydroxyapatite nanoparticles | |
| Bhattarai et al. | Electrospinning: how to produce nanofibers using most inexpensive technique? An insight into the real challenges of electrospinning such nanofibers and its application areas | |
| CN101831762A (en) | Composite fiber porous membrane composited by ornithoctonus huwena threads and polylactic acid and method for producing same | |
| Cheng et al. | Controllable preparation and formation mechanism of nanofiber membranes with large pore sizes using a modified electrospinning | |
| CN107496992B (en) | Three-dimensional patterned Fe3O4Medical polymer material composite nanofiber and preparation method thereof | |
| CN112774457A (en) | Polymer microfiltration membrane and preparation method and application thereof | |
| CN106087078A (en) | A kind of receptor for electrostatic spinning, electrostatic spinning apparatus, there is the preparation method of the three-dimensional manometer fibre structure of multi-stage porous | |
| CN106390773A (en) | Self-supported attapulgite nanofiber membrane, and preparation method thereof | |
| CN113215670A (en) | Dipping rotary spinning equipment for preparing gelatin/cellulose 3D bracket for cell culture meat production | |
| CN103638561B (en) | Preparation method of micro-nano bioactive porous material | |
| CN106012297B (en) | A kind of preparation method of medical composite fibre three-dimensional structure dressing | |
| CN105420927B (en) | A kind of efficiently adjustable mixing liquid separation tunica fibrosa and preparation method thereof | |
| Chen et al. | Advanced functional nanofibers: strategies to improve performance and expand functions | |
| CN107475793A (en) | A kind of preparation method of graphene oxide parcel polyacrylonitrile composite nano fiber | |
| CN106283399A (en) | A kind of arrange orderly modified nano fiber film and preparation thereof and application | |
| CN110863349A (en) | Preparation method of centrifugal spinning nanofiber body material | |
| CN102499799A (en) | Cardiac stent with three-dimensional structure and anisotropy and preparation method thereof | |
| Tang et al. | Preparation of fiber-microsphere scaffolds for loading bioactive substances in gradient amounts |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |