CN102499799A - Cardiac stent with three-dimensional structure and anisotropy and preparation method thereof - Google Patents
Cardiac stent with three-dimensional structure and anisotropy and preparation method thereof Download PDFInfo
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- CN102499799A CN102499799A CN2011103449150A CN201110344915A CN102499799A CN 102499799 A CN102499799 A CN 102499799A CN 2011103449150 A CN2011103449150 A CN 2011103449150A CN 201110344915 A CN201110344915 A CN 201110344915A CN 102499799 A CN102499799 A CN 102499799A
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Abstract
The invention relates to a cardiac stent with a three-dimensional structure and anisotropy and a preparation method thereof. According to the cardiac stent, a nanometer fiber stent is weaved by static spinning, a meshed structure is defined by adjusting the orientation, density and length of spinning fibers, suspended nanometer fibers without a substrate allow the attachment and growth of cells, and an excitable mesh is formed by the nanometer fiber stent in a hollow tissue form. The cardiac stent has the advantages that: fiber materials prepared by the method can be used for a system for constructing cardiac tissues in vitro, and the anisotropy of the cells is controlled by aggregating the cells on the nanometer fiber mesh for growing; the nanometer fiber mesh is covered on a support made from polydimethylsiloxane by electric spinning to be suspended in the air; and the three-dimensional structure and the anisotropy can be kept, so that the transmission of nutrient and metabolic products is facilitated by a polymethyl glutarimide fiber-based tissue structure, and an ideal system is provided for tissue engineering. A preparation process is simple and convenient to operate, and has a bright application prospect.
Description
Technical field:
The present invention relates to heart nano fiber scaffold and preparation method thereof, more specifically, is a kind of have three dimensional structure and anisotropic cardiac stent and preparation method thereof.
Background technology:
Organizational project is a field that develops rapidly, and it relies on the continuous progress of tissue culture and material science, and both are merged.The organizational project purpose is to make organ specific organizational structure to carry out regenerative medicine, like implantable tissue and organ.The important feature of heart tissue is the multiformity of its highly developed 26S Proteasome Structure and Function, and its perfect orthofunction can make the myocardial cell can be as a pump, but this has also increased the complexity of heart tissue engineering strategy.
Cultivating the monolayer myocardial cell is a kind of basic skills of regenerative medicine.Attempt the complexity of natural imitation cardiac muscle through cultivating orderly cell anisotropic monolayer.Guarantee that through the help of microetch or microflute or collagen protein cell aligns with substrate, accurately orderly fibronectin.In this way, orderly heart single layer structure is by the pattern decision of the solid substrate of sustenticular cell growth.Recover the contractility of damaged myocardium, the organizational structure of implantation should possess enough contractilitys and mechanical strength.Obviously, solid substrate can not satisfy these requirements fully for the myocardial cell monofilm on basis.
When being embedded in three-dimensional environment, we hope that cell forms the structure of high-sequential, have contact and extracellular matrix (ECM) in the cell.In organizational project, the effect of ECM is substituted by the mounting system that satisfies some requirement, and it must have biocompatibility, biodegradability, favorable mechanical performance.As the ECM of heart tissue, it must guarantee that the cell with contractile function does not have impedance.
The mounting system that is the basis with the electrostatic spinning nano fiber is carried out the cell arrangement and has been obtained good effect.He can support cell guiding through the change of multiple kinds of molds, comprises cardiac nerve, blood vessel and myocardial cell.Nano fiber scaffold is used with the shape of mat or cotton shape ball usually.Yet the structure that must control entire bracket is guaranteed being connected between cell and the cell, constitutes the complete function that stimulates network, and this is the prerequisite that heart tissue runs well.This is a very big challenge for thicker support, is this area problem demanding prompt solution.
Summary of the invention:
The object of the present invention is to provide a kind of have three dimensional structure and anisotropic cardiac stent and preparation method thereof.
To achieve these goals, the present invention proposes following technical scheme realization:
A kind of have three dimensional structure and an anisotropic cardiac stent, and it is characterized in that: nano fiber scaffold is made into through electrostatic spinning, and define RF through adjustment spinning fibre orientation, fibre density, fibre length; The nanofiber of the no substrate of suspension allows adhering to of cell and grows, and the type of organization of nano fiber scaffold hollow out constitutes can excited network.
The present invention prepares the method for preparing of above-mentioned heart nano fiber scaffold, comprises the steps:
(1) polydimethylsiloxane is processed an end in the culture dish of plane, after depositing 2-10 hour under 50-100 ℃, separates from culture dish, cleans with ethanol before using;
(2) will gather polydimethyl glutarimide is dissolved in and processes the solution that concentration is 10-20% in Ketocyclopentane and the tetrahydrofurfuryl alcohol; Again the ionic surface active agent sodium lauryl sulphate being dissolved in ethanol, to obtain concentration be that the solution blending of 0.1-2g/L advances in the polymer solution, is mixed with spinning liquid;
(3) with the spinning liquid 1-5ml syringe of packing into, be 1-10ml/h with flow speed control through syringe pump, voltage 5-15kv; The distance of gathering-device and syringe tip is 10-20cm, and gathering-device is circular, diameter 5-15cm; With the thick aluminium foil manufacturing of 50-200mm, through the arrangement of the intermediary rectangular opening control of catcher nanofiber, the width of adjustment hole is 2-20mm; Be 20-25mm highly, be used to control fibre length, control the density of nanofiber through the time 0.5-2min that changes fiber laydown; Behind the electrostatic spinning, gather the polydimethyl glutarimide fiber and transfer on the support of polydimethylsiloxane, and under vacuum state, preserve 12-48 hour to remove residual solvent from gathering-device.
Beneficial effect of the present invention: can be used for the system of external structure heart tissue according to the fibrous material of the present invention preparation, control the anisotropic of cell in the online growth of polymeric nanofiber through making cell; Nanometer fiber net spins the holder that covers by the dimethione manufacturing through electricity and is suspended in the air; Compared with prior art; Can keep three dimensional structure and anisotropy; Make and gather the polydimethyl glutarimide fiber and be beneficial to through nutrient and metabolite, thereby an ideal system is provided for organizational project for the organizational structure on basis; And preparation technology is simple, easy to operate, has broad application prospects.
The specific embodiment:
Below describe preferred implementation of the present invention, but be not in order to limit the present invention.
Embodiment 1:
Prepare nano fiber scaffold according to following method:
(1) a preparation end: polydimethylsiloxane (PDMS) on the plane culture dish mixed, after having deposited 5 hours under 80 ℃, separate from culture dish, ethanol cleans before using.
(2) preparing spinning solution: will gather polydimethyl glutarimide (PMGI) and be dissolved in that to process concentration in Ketocyclopentane and the tetrahydrofurfuryl alcohol be 13% solution; Again the ionic surface active agent sodium lauryl sulphate being dissolved in ethanol, is that 0.48g/L mixes in the polymer solution with the final concentration.
(3) electrostatic spinning and collection: with the spinning liquid 1ml syringe of packing into, be 2ml/h with flow speed control through syringe pump, voltage 8kv; The distance of gathering-device and syringe tip is 10cm, and gathering-device is circular, diameter 5cm; With the thick aluminium foil manufacturing of 100mm; Through the arrangement of the intermediary rectangular opening control of catcher nanofiber, the width control fibre length of adjustment hole is a density (fiber height that collect in every hole) of the spray webbing time controlling nanofiber through the time that changes fiber laydown; Behind the electrostatic spinning, the PMGI fiber is transferred on the support of PDMS from gathering-device, and under vacuum state, preserves 24 hours to remove residual solvent.
Claims (2)
1. one kind has three dimensional structure and anisotropic cardiac stent; It is characterized in that: nano fiber scaffold is made into through electrostatic spinning; And defining RF through adjustment spinning fibre orientation, fibre density, fibre length, the type of organization of nano fiber scaffold hollow out constitutes can excited network.
2. method for preparing with three dimensional structure and anisotropic cardiac stent may further comprise the steps:
(1) polydimethylsiloxane is processed an end in the culture dish of plane, after depositing 2-10 hour under 50-100 ℃, separates from culture dish, cleans with ethanol before using.
(2) will gather polydimethyl glutarimide is dissolved in and processes the solution that concentration is 10-20% in Ketocyclopentane and the tetrahydrofurfuryl alcohol; Again the ionic surface active agent sodium lauryl sulphate being dissolved in ethanol, to obtain concentration be that the solution blending of 0.1-2g/L advances in the polymer solution, is mixed with spinning liquid.
(3) with the spinning liquid 1-5ml syringe of packing into, be 1-10ml/h with flow speed control through syringe pump, voltage 5-15kv; The distance of gathering-device and syringe tip is 10-20cm, and gathering-device is circular, diameter 5-15cm; With the thick aluminium foil manufacturing of 50-200mm, through the arrangement of the intermediary rectangular opening control of catcher nanofiber, the width of adjustment hole is 2-20mm; Be 20-25mm highly, be used to control fibre length, control the density of nanofiber through the time 0.5-2min that changes fiber laydown; Behind the electrostatic spinning, gather the polydimethyl glutarimide fiber and transfer on the support of polydimethylsiloxane, and under vacuum state, preserve 12-48 hour to remove residual solvent from gathering-device.
Priority Applications (1)
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| CN2011103449150A CN102499799A (en) | 2011-11-04 | 2011-11-04 | Cardiac stent with three-dimensional structure and anisotropy and preparation method thereof |
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| CN2011103449150A CN102499799A (en) | 2011-11-04 | 2011-11-04 | Cardiac stent with three-dimensional structure and anisotropy and preparation method thereof |
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| CN102499799A true CN102499799A (en) | 2012-06-20 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103861147A (en) * | 2014-03-27 | 2014-06-18 | 杭州市第三人民医院 | Method for culturing human melanocyte based on nano-fiber scaffold |
| CN104694467A (en) * | 2013-12-04 | 2015-06-10 | 中国科学院大连化学物理研究所 | One-dimensional cell growth method based on nano electrospinning |
| CN104774762A (en) * | 2015-03-31 | 2015-07-15 | 苏州睿研纳米医学科技有限公司 | Oriented polymer nanometer fibrocyte culture plate and preparation method thereof |
| CN109983342A (en) * | 2016-08-11 | 2019-07-05 | 再心生物科技有限公司 | System and method for simulating disease and assessing its treatment side effect |
Citations (2)
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| CN1895687A (en) * | 2006-06-09 | 2007-01-17 | 浙江大学 | Netted tissue-engineering stand |
| CN101912318A (en) * | 2010-07-30 | 2010-12-15 | 东华大学 | Three-layer electrostatic spinning ordered fiber nerve conduit and preparation and application thereof |
-
2011
- 2011-11-04 CN CN2011103449150A patent/CN102499799A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1895687A (en) * | 2006-06-09 | 2007-01-17 | 浙江大学 | Netted tissue-engineering stand |
| CN101912318A (en) * | 2010-07-30 | 2010-12-15 | 东华大学 | Three-layer electrostatic spinning ordered fiber nerve conduit and preparation and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 陈锐: "静电纺制备胶原蛋白/聚氨酯心脏瓣膜组织工程支架材料的研究", 《中国博士学位论文全文数据库 医药卫生科技辑》, no. 8, 15 August 2011 (2011-08-15) * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104694467A (en) * | 2013-12-04 | 2015-06-10 | 中国科学院大连化学物理研究所 | One-dimensional cell growth method based on nano electrospinning |
| CN104694467B (en) * | 2013-12-04 | 2017-12-01 | 中国科学院大连化学物理研究所 | A kind of cell one-dimensional growth method based on nanometer Electrospun |
| CN103861147A (en) * | 2014-03-27 | 2014-06-18 | 杭州市第三人民医院 | Method for culturing human melanocyte based on nano-fiber scaffold |
| CN103861147B (en) * | 2014-03-27 | 2015-10-28 | 杭州市第三人民医院 | A kind of cultural method of the human melanocyte based on nano fiber scaffold |
| CN104774762A (en) * | 2015-03-31 | 2015-07-15 | 苏州睿研纳米医学科技有限公司 | Oriented polymer nanometer fibrocyte culture plate and preparation method thereof |
| CN109983342A (en) * | 2016-08-11 | 2019-07-05 | 再心生物科技有限公司 | System and method for simulating disease and assessing its treatment side effect |
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Application publication date: 20120620 |