CN107494964A - A kind of probiotic composition and its application - Google Patents
A kind of probiotic composition and its application Download PDFInfo
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- CN107494964A CN107494964A CN201710905031.5A CN201710905031A CN107494964A CN 107494964 A CN107494964 A CN 107494964A CN 201710905031 A CN201710905031 A CN 201710905031A CN 107494964 A CN107494964 A CN 107494964A
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- 239000000203 mixture Substances 0.000 title claims abstract description 59
- 239000006041 probiotic Substances 0.000 title claims abstract description 39
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 39
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 37
- 206010012735 Diarrhoea Diseases 0.000 claims abstract description 12
- 230000036528 appetite Effects 0.000 claims abstract description 6
- 235000019789 appetite Nutrition 0.000 claims abstract description 6
- 239000000654 additive Substances 0.000 claims description 2
- 230000000996 additive effect Effects 0.000 claims description 2
- 230000033228 biological regulation Effects 0.000 claims description 2
- 241000218588 Lactobacillus rhamnosus Species 0.000 abstract description 10
- 240000001046 Lactobacillus acidophilus Species 0.000 abstract description 7
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 abstract description 7
- 229940039695 lactobacillus acidophilus Drugs 0.000 abstract description 7
- 210000000936 intestine Anatomy 0.000 abstract description 4
- 101100207371 Curvularia clavata TR08 gene Proteins 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 2
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 abstract 1
- 241001608472 Bifidobacterium longum Species 0.000 abstract 1
- 229920001202 Inulin Polymers 0.000 abstract 1
- 241000186605 Lactobacillus paracasei Species 0.000 abstract 1
- 240000006024 Lactobacillus plantarum Species 0.000 abstract 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 abstract 1
- 229940009289 bifidobacterium lactis Drugs 0.000 abstract 1
- 229940009291 bifidobacterium longum Drugs 0.000 abstract 1
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 abstract 1
- 235000013325 dietary fiber Nutrition 0.000 abstract 1
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 abstract 1
- 229940029339 inulin Drugs 0.000 abstract 1
- 229940072205 lactobacillus plantarum Drugs 0.000 abstract 1
- 230000035611 feeding Effects 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 9
- 239000003814 drug Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
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- 230000000968 intestinal effect Effects 0.000 description 4
- 229940079593 drug Drugs 0.000 description 3
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- 239000008267 milk Substances 0.000 description 2
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- 206010047700 Vomiting Diseases 0.000 description 1
- PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
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- 238000010171 animal model Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229940054340 bacillus coagulans Drugs 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
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- 239000003795 chemical substances by application Substances 0.000 description 1
- 208000021735 chronic enteritis Diseases 0.000 description 1
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
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Classifications
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- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/702—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/733—Fructosans, e.g. inulin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K35/74—Bacteria
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- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
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-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/165—Paracasei
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/531—Lactis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/533—Longum
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a kind of probiotic composition, contains per 100g：Dietary fiber 35~80%；Inulin 15~60%；Xylo-oligosaccharide 5~15%；The CFU of lactobacillus acidophilus 10~5,000,000,000；The CFU of Lactobacillus rhamnosus TR08 10~3,000,000,000；The CFU of Lactobacillus plantarum 20~4,000,000,000；The CFU of Lactobacillus paracasei 10~3,000,000,000；The CFU of bifidobacterium longum 10~2,000,000,000；The CFU of bifidobacterium lactis 10~3,000,000,000.Composition preparation technology provided by the invention is simple, cost is cheap, easy to use, can improve pet appetite, improve pet intestine microenvironment, preventing and treating pet diarrhoea, improves pet immunocompetence.
Description
Technical field
The invention belongs to biological technical field, more particularly to a kind of probiotic composition, the application of said composition is further related to.
Background technology
With the improvement of people's living standards, the people of raising pet is more and more, the feed request that pet needs is also more next
More become more meticulous.But, on the one hand, present feed for pet raw material are essentially identical, and main component is similar, long-term consumption, can cause
Poor appetite, gasteremphraxis, pet dull-looking, hair clouding, coarse, off color, often sleep, causes sick；On the other hand,
The dog food of domestic pet long-term consumption, cat food, snacks etc. are processed food, and the intake of various food additives can sustaining breakdown
Pet intestines and stomach colony balance, easily produce constipation, have loose bowels, the gastrointestinal disease such as indigestion, enterogastritis.Sick typically upper pet
Hospital, but prolonged administration of drugs can cause dependence, while medicine is chemical synthesis, there is more or less pair to make
With, and pet typically compares resistance and taken medicine, and has an injection.In addition, there is not the feed addictive for being available for pet to be eaten for a long time at present yet
To solve problem of the prior art.
The content of the invention
Technical problem：In order to solve the defects of prior art, the invention provides a kind of pet probiotic composition.
Technical scheme：A kind of probiotic composition provided by the invention, contain per 100g：
Preferably, the probiotic composition, contains per 100g：
As another kind preferably, the probiotic composition, contain per 100g：
Present invention also offers application of the above-mentioned probiotic composition in feed for pet additive is prepared.
Present invention also offers above-mentioned probiotic composition to prepare the feed addictive of regulation pet gut flora balance
In application.
Present invention also offers application of the above-mentioned probiotic composition in the feed addictive for preparing preventing and treating pet diarrhoea.
Present invention also offers application of the above-mentioned probiotic composition in the feed addictive for improving pet appetite is prepared.
Present invention also offers above-mentioned probiotic composition in the feed addictive for improving pet immunocompetence is prepared
Using.
Present invention also offers above-mentioned probiotic composition in the feed addictive for improving pet immunocompetence is prepared
Using.
Beneficial effect：Composition preparation technology provided by the invention is simple, cost is cheap, easy to use, can improve pet
Appetite, improve pet intestine microenvironment, preventing and treating pet diarrhoea, improve pet immunocompetence.
Specifically, probiotic composition of the present invention has advantage following prominent relative to prior art：
The probiotic composition improves pet intestine microenvironment by following principle：
(1) supplement：Said composition is combined by quickly supplementing more effective activity of probiotic first, including is come from enteron aisle
Lactic acid bacteria, Bifidobacterium and in enteron aisle breeding create environment bacillus coagulans, the probiotic combinations are high work
Property, high field planting rate beneficial bacteria of intestinal tract vigor combination, can quickly be supplemented in enteron aisle, enteral probiotics quantity is recovered to health
It is horizontal.
(2) suppress：Probiotics in said composition can effectively suppress to damage function of intestinal canal, induce the harmful of various diseases
Bacterium；Specific activity of probiotic combines the oxygen by capturing harmful bacteria living necessities, produces the lactic acid that can suppress harmful bacteria growing,
Capture adhesion locations of the harmful bacteria in enteron aisle, the multi-faceted growth for effectively suppressing harmful bacteria, restorative intestinal microecology ring
Border.
(3) rebuild：Said composition can reconstruction enteron aisle biological barrier, enhancing is immune, promotes nutrient absorption.Said composition
It can be colonized in gut mucosal surface, repair and rebuild destroyed enteron aisle biological barrier, prevent the invasion of pathogenic bacteria and toxin, lure
Lead intestinal mucosa and produce various antibody, enhancing is immune, really makes enteron aisle long-term stability.
(4) cooperate with：Said composition possesses outstanding cooperative ability, and can arrange in pairs or groups clothes for a long time with a variety of medicines, nutriment, food
With promoting absorption of the body to nutrition, mitigate the toxic side effect of multi-medicament, allow enteron aisle to add vigor doubly.
The probiotic composition purposes is widely used：
(1) said composition can be used for adjusting gut flora, protect stomach, suppress harmful bacteria growing, to pet diarrhoea, just
Secret, acute chronic enteritis have good efficacy；
(2) convalescence that said composition can be used for after pet operation and antibiosis extract for treating, intestinal microecology can be improved rapidly
Environment, improve health conditions；
(3) said composition can promote digestion and absorb, and eliminating pet halitosis caused by gastrointestinal disorder reduces excretion foul smell,
Excreta is molded, improves feeding environment；
(4) said composition can significantly improve pet partial eclipse particular about food, promote a variety of digestive ferment vitamins and nutriment in vivo
Generation, strengthen immunity；
(5) said composition can reduce dog cat because wean reloads, immune expelling parasite, diarrhoea etc. is various caused by environmental change stress
Reaction.
Brief description of the drawings
Fig. 1 is to detect that lactobacillus acidophilus carries out counting knot to the lactobacillus acidophilus in excrement according to strain specific PCR
Fruit is schemed.
Embodiment
Probiotic composition of the present invention is further illustrated below.
Lactobacillus rhamnosus is Lactobacillus rhamnosus (L.rhamnosus) TR08, and its Classification And Nomenclature is Lactobacillus rhamnosus
(Lactobacillus rhamnosus) TR08, in China typical culture collection center (abbreviation CCTCC) preservation, preservation list
Bit address：Wuhan City, Hubei Province Wuchang District Wuhan University, postcode 430072, its deposit number are：CCTCC NO.M
2016548, preservation date is on October 10th, 2016.
Embodiment 1
Probiotic composition, contain per 100g：
Embodiment 2
Probiotic composition, contain per 100g：
Embodiment 3
Probiotic composition, contain per 100g：
Embodiment 4
Probiotic composition, contain per 100g：
Embodiment 5
Probiotic composition, contain per 100g：
Embodiment 6
Probiotic composition, contain per 100g：
The pharmacodynamic evaluation of the present composition.
Experimental agents：The composition of embodiment 1 to 6；
Experimental animal：20 age in days pups 210, it is divided into 7 groups (experimental groups 1 to experimental group 6, control group), gives reality respectively
Test medicine and control drug.
Feeding method：
(1) experimental group：20~30 ages in days feed the milk powder for being mixed with the present composition；Feeding is mixed with multiple after the ablactation of 30 ages in days
The feed of lactic acid bacteria is closed, with 2 times/day of feedings；
(2) control group：20~30 ages in days feed milk powder；Feeding feed stuff after the ablactation of 30 ages in days, with 2 times/day of feedings.
It is alternative to select 5 pups, detect that lactobacillus acidophilus enters to the lactobacillus acidophilus in excrement according to strain specific PCR
Row counts, the 1st day to the 3rd day, gives the Yoghourt of dog feeding (1.7 hundred million CFU/ days) containing lactobacillus acidophilus, collects fecal sample respectively,
Detected, as a result, see Fig. 1, show：Lactobacillus acidophilus can be effectively in the existence of dog enteron aisle.
The pup blood parameters of test experience group and control group, are shown in Table 1.
The pup blood parameters of the experimental group of table 1 and control group
The test experience group and pup contents of mda of control group, SOD and GSH-PX activity, are shown in Table 2.
The experimental group of the table 2 and pup contents of mda of control group, SOD and GSH-PX activity
| Project | MDA/umol.ml-1 | SOD/U.ml-1 | GSH-PX/U.ml-1 |
| Experimental group 1 | 5.66±0.83 | 19.80±9.00 | 2266.33±281.53 |
| Experimental group 2 | 5.71±0.61 | 19.18±12.60 | 2200.38±187.77 |
| Experimental group 3 | 5.65±0.81 | 19.68±10.46 | 2246.28±195.82 |
| Experimental group 4 | 5.70±0.76 | 19.57±11.51 | 2249.64±218.6 |
| Experimental group 5 | 5.67±0.79 | 19.91±10.89 | 2219.78±268.4 |
| Experimental group 6 | 5.28±0.56 | 20.76±10.82 | 2408.92±192.58 |
| Control group | 6.98±0.81 | 16.05±6.64 | 1904.95±128.28 |
Result above shows：
(1) after using present composition feeding pup, the blood total protein of pup, the increase of globulin content, and Archon
Than reducing, show：The present composition can improve the immunocompetence of pup body.
(2) after using present composition feeding pup, Plasma Urea nitrogen content reduces, and shows：The present composition can
Pup is improved to the anabolism of protein, adds deposition of the nitrogen in pup body.
(3) present composition can promote intestinal movement, strengthen digestive function, improve hormonal readiness in blood, promote machine
The anabolism of body, stimulating animal immunity function, trophism is played by immunization route.
(4) after using present composition feeding pup, pup blood SOD and GSH-PX activity improve, and MDA contents are notable
Decline, show：The present composition can improve the immunocompetence and oxidation resistance of pup.
Pup diarrhoea time and degree are counted, is shown in Table 3.
The pup of table 3 diarrhoea time and degree
| Group | Severe | Slightly | Amount to |
| Experimental group 1 | 0 | 3 | 3 |
| Experimental group 2 | 0 | 2 | 2 |
| Experimental group 3 | 0 | 3 | 3 |
| Experimental group 4 | 0 | 3 | 3 |
| Experimental group 5 | 0 | 2 | 2 |
| Experimental group 6 | 0 | 0 | 0 |
| Control group | 3 | 12 | 15 |
Wherein, the time is suffered from diarrhoea to be less than 1 day (containing) slight, and the diarrhoea time is that more than 1 day (being free of) is severe.
Each period pup body weight is counted, is shown in Table 4.
Each period pup body weight of table 4
The pup death rate is counted, is shown in Table 5.
The pup death rate of table 5
| Group | 20 | 30 | 40 | 50 | 60 | Death toll | The death rate/% |
| Experimental group 1 | 0 | 1 | 1 | 0 | 0 | 2 | 6.67 |
| Experimental group 2 | 1 | 1 | 0 | 0 | 0 | 2 | 6.67 |
| Experimental group 3 | 1 | 0 | 1 | 0 | 0 | 2 | 6.67 |
| Experimental group 4 | 1 | 0 | 0 | 0 | 0 | 1 | 3.33 |
| Experimental group 5 | 0 | 1 | 1 | 0 | 0 | 2 | 6.67 |
| Experimental group 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0.00 |
| Control group | 1 | 3 | 5 | 0 | 0 | 9 | 30.0 |
Result above shows：After present composition feeding pup, the young dog diarrhoea time substantially reduces, increased weight
Comparatively fast, survival rate is higher.
Meanwhile found in experimentation：After present composition feeding pup, the appetite of pet can be improved, is subtracted
Few vomiting reaction.
Sequence table
<110>Tian Yi bio tech ltd of Zhengjiang City
<120>A kind of probiotic composition and its application
<130> SC20161008002
<150> 2017104459778
<151> 2017-06-14
<160> 2
<170> SIPOSequenceListing 1.0
<210> 2
<211> 1474
<212> DNA
<213> Lactobacillus rhamnosus
<220>
<221> misc_feature
<222> (1)..(1474)
<400> 2
cgctggcggc gtgcctaata catgcaagtc gaacgagttc tgattattga aaggtgcttg 60
catcttgatt taattttgaa cgaatggcgg aagggtgaat aacccgtggg taacctggcc 120
ttaaatgggg gataaccttt ggaaaccgaa gctaataccg cataaatccc aaaaccgcat 180
ggttcttggc tgaaagatgg ggtaaactat cccttttgga tggacccccg gcgtattaac 240
ttgttggtga ggtaacggct ccaccaggga atgataccta acccaactga aaggttgatc 300
ggccaccttg ggactgagac ccggcccaaa ctcctaccgg agggagcaat agggaatctt 360
ccccaatgga cgccagtctg aaggaacaac cccccgtggg tgaaaaaggg tttccggtcc 420
taaaactctg ttgttggaaa aaaatggtcc gcaaaataac tggtggccgc gtggccgtat 480
ccaacccgaa agccacggct aactacctgc ccagcaccgc ggtaataccg aggtggcaag 540
cgttatccgg atttattggg cgtaaagcga gcgcaagcgg gttttttagt ctgatgggaa 600
agccctcggc ttaaccgaag aagtgcatcg gaaactggga aacttgaatg cagaagaaga 660
cagtggaact ccatgtgtag cggtgaaatg cctagatata tggaagaaca ccagtggcga 720
aagccgctgt ctggtctgta actgacgctg aagctccaaa gcctgggtag cgaacaggat 780
tagataccct gggagtccat gccgtaacga tgaatgctag tggtggaagg tttccgccct 840
tcaatgccga gcttacgcaa taagcaatcc gcctggggga atacgaccgg caggttgaaa 900
cttcaaagaa tttgaccggg gccgcacaag ccggtggagc attgtggttt aattcgaagc 960
acgcgaagaa cttaccaggt cttgacatct tttgatcact tgaagatcaa gtttcccttc 1020
gggcaaatga cagtggtgca tggttgtcgt cagctccgtg tcgtgagatg ttgggttaag 1080
tcccgcaacg agcgcaaccc ttatgactag ttgccagcat ttagttgggc actctagtaa 1140
gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg 1200
acctgggcta cacacgtgct acaatggatg gtacaacgag ttgcgagacc gcgaggtcaa 1260
gctaatctct taaagccatt ctcagttcgg actgtaggct gcaactcgcc tacacgaagt 1320
cggaatcgct agtaatcgcg gatcagcacg ccgcggtgaa tacgttcccg ggccttgtac 1380
acaccgcccg tcacaccatg agagtttgta acacccgaag ccggtggcgt aaccctttta 1440
gggagcgagc cgtctaaggt ggacaaatga tact 1474
<210> 3
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<212> DNA
<213>Lactobacillus rhamnosus is Lactobacillus rhamnosus (L.rhamnosus TR08Lactobacillus reuteri)
<220>
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<222> (1)..(1490)
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gggtacgcgg cggtgtgcta tacatgcaag tcgtacgcac tggcccaact gattgatggt 60
gcttgcacct gattgacgat ggattaccag tgagtggcgg acgggtgagt aacacgtagg 120
taacctgccc cggagcgggg gataacattt ggaaacagat gctaataccg cataacaaca 180
aaagccacat ggcttttgtt tgaaagatgg ctttggctat cactctggga tggacctgcg 240
gtgcattagc tagttggtaa ggtaacggct taccaaggcg atgatgcata gccgagttga 300
gagactgatc ggccacaatg gaactgagac acggtccata ctcctacggg aggcagcagt 360
agggaatctt ccacaatggg cgcaagcctg atggagcaac accgcgtgag tgaagaaggg 420
tttcggctcg taaagctctg ttgttggaga agaacgtgcg tgagagtaac tgttcacgca 480
gtgacggtat ccaaccagaa agtcacggct aactacgtgc cagcagccgc ggtaatacgt 540
aggtggcaag cgttatccgg atttattggg cgtaaagcga gcgcaggcgg ttgcttaggt 600
ctgatgtgaa agccttcggc ttaaccgaag aagtgcatcg gaaaccgggc gacttgagtg 660
cagaagagga cagtggaact ccatgtgtag cggtggaatg cgtagatata tggaagaaca 720
ccagtggcga aggcggctgt ctggtctgca actgacgctg aggctcgaaa gcatgggtag 780
cgaacaggat tagataccct ggtagtccat gccgtaaacg atgagtgcta ggtgttggag 840
ggtttccgcc cttcagtgcc ggagctaacg cattaagcac tccgcctggg gagtacgacc 900
gcaaggttga aactcaaagg aattgacggg ggcccgcaca agcggtggag catgtggttt 960
aattcgaagc tacgcgaaga accttaccag gtcttgacat cttgcgctaa ccttagagat 1020
aaggcgttcc cttcggggac gcaatgacag gtggtgcatg gtcgtcgtca gctcgtgtcg 1080
tgagatgttg ggttaagtcc cgcaacgagc gcaacccttg ttactagttg ccagcattaa 1140
gttgggcact ctagtgagac tgccggtgac aaaccggagg aaggtgggga cgacgtcaga 1200
tcatcatgcc ccttatgacc tgggctacac acgtgctaca atggacggta caacgagtcg 1260
caagctcgcg agagtaagct aatctcttaa agccgttctc agttcggact gtaggctgca 1320
actcgcctac acgaagtcgg aatcgctagt aatcgcggat cagcatgccg cggtgaatac 1380
gttcccgggc cttgtacaca ccgcccgtca caccatggga gtttgtaacg cccaaagtcg 1440
gtggcctaac ctttatggag ggagccgcct aaggcggaca gagactgggt 1490
Claims (9)
- A kind of 1. probiotic composition, it is characterised in that：Contain per 100g：
- A kind of 2. probiotic composition according to claim 1, it is characterised in that：Contain per 100g：
- A kind of 3. probiotic composition according to claim 1, it is characterised in that：Contain per 100g：
- 4. application of the probiotic composition in feed for pet additive is prepared described in claim 1.
- 5. probiotic composition the answering in the feed addictive for preparing regulation pet gut flora balance described in claim 1 With.
- 6. application of the probiotic composition in the feed addictive for preparing preventing and treating pet diarrhoea described in claim 1.
- 7. application of the probiotic composition in the feed addictive for improving pet appetite is prepared described in claim 1.
- 8. application of the probiotic composition in the feed addictive for improving pet immunocompetence is prepared described in claim 1.
- 9. application of the probiotic composition in the feed addictive for improving pet immunocompetence is prepared described in claim 1.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108323641A (en) * | 2018-02-07 | 2018-07-27 | 上海宠幸宠物用品有限公司 | A kind of health food and preparation method thereof for improving pet dog cat function of intestinal canal |
| CN109043258A (en) * | 2018-07-25 | 2018-12-21 | 重庆正通生物发展有限公司 | A kind of dietary fiber plant beverage and preparation method thereof and eating method |
| CN109363183A (en) * | 2018-12-11 | 2019-02-22 | 大连民族大学 | A kind of inulin probiotics companion and preparation method thereof |
| CN109549957A (en) * | 2018-11-29 | 2019-04-02 | 吉林禾未来功能产品有限公司 | The compound probiotic of energy bidirectional modulation constipation |
| CN117958363A (en) * | 2024-03-28 | 2024-05-03 | 北京和益源生物技术有限公司 | Probiotic cat food for improving digestion function of pet cats and preparation method thereof |
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| GB201719480D0 (en) * | 2017-11-23 | 2018-01-10 | Optibiotix Ltd | Compositions |
| CN107868764A (en) * | 2017-11-27 | 2018-04-03 | 内蒙古普泽生物制品有限责任公司 | A kind of compound probiotic powder preparation for strengthening immunity of pets and preparation method thereof |
| CN108077935A (en) * | 2018-01-22 | 2018-05-29 | 广东格物生物科技有限公司 | Nurse one's health middle-aged and elderly people's function of intestinal canal microbial inoculum and its preparation method and application |
| CN108902517A (en) * | 2018-05-16 | 2018-11-30 | 浙江省中医药研究院 | A kind of probiotic composition and its application |
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| CN108323641A (en) * | 2018-02-07 | 2018-07-27 | 上海宠幸宠物用品有限公司 | A kind of health food and preparation method thereof for improving pet dog cat function of intestinal canal |
| CN109043258A (en) * | 2018-07-25 | 2018-12-21 | 重庆正通生物发展有限公司 | A kind of dietary fiber plant beverage and preparation method thereof and eating method |
| CN109549957A (en) * | 2018-11-29 | 2019-04-02 | 吉林禾未来功能产品有限公司 | The compound probiotic of energy bidirectional modulation constipation |
| CN109363183A (en) * | 2018-12-11 | 2019-02-22 | 大连民族大学 | A kind of inulin probiotics companion and preparation method thereof |
| CN117958363A (en) * | 2024-03-28 | 2024-05-03 | 北京和益源生物技术有限公司 | Probiotic cat food for improving digestion function of pet cats and preparation method thereof |
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