CN107475167A - A kind of biomass carbon base soil-repairing agent and preparation method thereof - Google Patents
A kind of biomass carbon base soil-repairing agent and preparation method thereof Download PDFInfo
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- CN107475167A CN107475167A CN201710980192.0A CN201710980192A CN107475167A CN 107475167 A CN107475167 A CN 107475167A CN 201710980192 A CN201710980192 A CN 201710980192A CN 107475167 A CN107475167 A CN 107475167A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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Abstract
The invention discloses a kind of biomass carbon base soil-repairing agent and preparation method thereof, the biomass carbon base soil-repairing agent, is made up of the composition of following parts by weight：Nutrition powder is by 100 parts of total weight parts, weight ratio has objective microbe bacillus licheniformis, Rhodopseudomonas palustris, thuringiensis, photosynthetic bacteria, bacillus subtilis by the analysis for soybean powder of 23: 23: 5 mixing, mealy potato and powder of straw composition, preservation.The present invention can integrate the various conventional acid soil environments of adaptive, applied widely, and can be bred in self-growth under sour environment, administer timeliness length, reach the purpose that Long term control and continuous expanded scope are administered, and effect of integrated pest management is good；Or be desinsection or be getting fat or be anti-poor antitoxin or be to promote growth, microorganism forms the comprehensive function of complete cover crop, getting fat soil.
Description
Technical field
The present invention relates to restoration of the ecosystem field, more particularly to a kind of biomass carbon base soil-repairing agent and preparation method thereof.
Background technology
Soil remediation is the technical measures for making to recover by contaminated soil normal function.It is existing in soil remediation industry
Soil restoring technology reach kind more than 100, common technology also has kind more than ten, is broadly divided into physics, chemistry and biological three kinds of sides
Method.Since the 1980s, in the world it is many country particularly developed countries formulate and carried out contaminated soil administer with
Rehabilitation plan, therefore also form an emerging soil remediation industry.
Acid soil is the soil general name that pH value is less than 7.Including soil such as laterite, red earth, red soil, yellow earth and dry red soil
Class.China tropical and subtropical region, widely distributed various red or yellow soil acid soils.When ground temperature is high, rainfall
Greatly, annual rainfall is more in more than 1500mm.The characteristics of this high temperature and rainy, damp and hot same season, make the weathering of soil and pedogenesis equal
Very strong, the circulation of biological substance is very rapid.Alkali height is unsaturated, and pH is typically in 4.5-6.Iron-aluminium oxide has bright simultaneously
Aobvious accumulation, soil acid are thin.
Urgently one kind can integrate the various conventional acid soil environments of adaptive in the market, applied widely, and can be in acidity
Self-growth is bred under environment, administers timeliness length, reaches the purpose that Long term control and continuous expanded scope are administered, comprehensive regulation effect
Fruit is good；Or be desinsection or be getting fat or be anti-poor antitoxin or be to promote growth, microorganism forms complete cover crop, increasing
The biomass carbon base soil-repairing agent preparation method of fertile soil.
The content of the invention
For drawbacks described above present in prior art, the present invention is intended to provide one kind can integrate the various conventional acids of adaptive
Property soil environment, it is applied widely, and can be bred in self-growth under sour environment, administer timeliness length, reach Long term control and
The purpose that continuous expanded scope is administered, effect of integrated pest management is good, or be desinsection or be getting fat or be to resist poor antitoxin or be promotion
Growth, microorganism form complete cover crop, the biomass carbon base soil-repairing agent preparation method of getting fat soil.
To achieve these goals, the present invention uses following technical scheme：A kind of biomass carbon base soil-repairing agent, by with
The composition composition of lower parts by weight：Nutrition powder presses 2-3 by 100 parts of total weight parts, weight ratio：2-3：The analysis for soybean powder of 5 mixing, potato
Powder and powder of straw composition, preservation have objective microbe bacillus licheniformis, Rhodopseudomonas palustris, thuringiensis, light
Close bacterium, bacillus subtilis.
2. the manufacture method of biomass carbon base soil-repairing agent according to claim 1, it is characterised in that including with
Lower step：
1. analysis for soybean powder, mealy potato and powder of straw nutrition powder progress high temperature are removed respectively using 150 DEG C -200 DEG C of temperature
Bacterium and baking remove water, and continue 2h-3h, make analysis for soybean powder, mealy potato and the charing of powder of straw part, carbonization quality is than accounting for gross mass
15%-20%, you can obtain stand-by charcoal base analysis for soybean powder, mealy potato and powder of straw respectively；
2. 1. stand-by charcoal base analysis for soybean powder, mealy potato and powder of straw that step is obtained are by weight 2-3： 2-3：5 ratio
Mechanical mixture is uniform, obtains stand-by nutrition powder；
3. by bacillus licheniformis, Rhodopseudomonas palustris, thuringiensis, photosynthetic bacteria, bacillus subtilis
Each 5 parts of the stand-by nutrition powder that respectively 2. implantation step obtains, and encapsulation preserves respectively；
4. the nutrition powder for being implanted with Rhodopseudomonas palustris is placed under 33 DEG C -38 DEG C of closed environment, 2 days -3 are incubated
My god；The nutrition powder for being implanted with photosynthetic bacteria is placed under 22 DEG C -25 DEG C of closed environment, is incubated -10 days 7 days；It will be implanted with
The nutrition powder of bacillus licheniformis is placed under 30 DEG C -32 DEG C of closed environment, is incubated -5 days 4 days；Withered grass gemma will be implanted with
The nutrition powder of bacillus is placed in the environment of 35 DEG C -38 DEG C, is incubated -4 days 3 days；The nutrition of thuringiensis will be implanted with
Powder is placed in the environment of 35 DEG C -38 DEG C, is incubated -3 days 2 days；
5. and then the nutrition powder that five kinds of preservations have objective microbe is all mixed simultaneously with remaining stand-by nutrition powder
Stir, obtain pending biomass carbon base soil-repairing agent；
6. 5. pending biomass carbon base soil-repairing agent that step is obtained is placed in lucifuge and divulged information, and 20 DEG C of environment temperature-
25 DEG C, -8 days 5 days in humidity 50%-70% environment, that is, obtain needed for biomass carbon base soil-repairing agent.
Compared with the prior art, the present invention has advantages below as a result of such scheme：Bacillus licheniformis is certainly
The microorganism that right boundary is widely present, source is wide, strong adaptability, is suitable for after the domestication of specific acidification culture medium
Dry soil environment, the bacterium spontaneous fermentation and breed in dry soil environment, constantly produce crystal toxin and Mtx toxoids,
Both toxin will not cause to poison to other life entities outside insect larva, and can natural decomposition, do not interfere with environment, and
Because the larvae protein content that it is killed is high, nutrient, getting fat soil can be decomposed by the microorganisms into again；Photosynthetic bacteria is optimal
It is about 5 (acidity) to breed pH value, therefore can be with long term survival simultaneously just with the pH value phase permeability curve of dry soil environment (4.5-6)
Quick breeding, photosynthetic bacteria quickly can decompose to crop extremely bad rhizome, at the same also can fast decoupled nutritional powder, simultaneously
Using sunlight and the photosynthesis of itself, produce a large amount of active inorganic trophoplasms and be supplied to crop nutrition, can be consumed reducing
While increase dry soil environment fertilizer degree, while its secretion can by way of extracellular precipitation curing heavy metal, reduce it
Harm to plant, and it is hardened to be not easy soil；Rhodopseudomonas palustris thalline is nutritious, and protein content is up to 65%,
And various bioactivators are rich in, and it is adaptable strong, it is resistant to the organic wastewater of high concentration and stronger decomposition and inversion
Ability, also there are certain tolerance and capacity of decomposition to poisonous substances such as phenol, cyanogen；Thuringiensis can produce two big toxoids, i.e.,
Endotoxin (parasporal crystal) and exotoxin, insect is set to stop feeding, last insect is because of starvation and is poisoned to death.Therefore the bacillus can
Microbial source low toxic pesticide is done, for preventing and treating a variety of evils of Orthoptera, coleoptera, Diptera, Hymenoptera, particularly Lepidoptera
Worm；Bacillus subtilis available protein, a variety of sugar and starch, decompose tryptophan and form indoles, and indoles is to promote plant life
Long important substance；From the foregoing, it will be observed that five kinds of effective microbes can integrate the various conventional acid soil environments of adaptive, the scope of application
Extensively, it and can be bred in self-growth under sour environment, administer timeliness length, reach the mesh that Long term control and continuous expanded scope are administered
, effect of integrated pest management is good；Or be desinsection or be getting fat or be anti-poor antitoxin or be to promote growth, five kinds of microorganisms are formed
The comprehensive function of complete cover crop, getting fat soil, therefore the present invention is a kind of outstanding biomass carbon base soil remediation
Agent.
Embodiment
Embodiment 1：
A kind of biomass carbon base soil-repairing agent, is made up of the composition of following parts by weight：Nutrition powder is by total weight parts
100kg, weight ratio have objective microbe lichens gemma bar by the analysis for soybean powder of 1: 1: 2 mixing, mealy potato and powder of straw composition, preservation
Bacterium, Rhodopseudomonas palustris, thuringiensis, the blue bacterium that quivers greatly (one kind of photosynthetic bacteria), bacillus subtilis.
The manufacture method of above-mentioned biomass carbon base soil-repairing agent, comprises the following steps：
1. using 180 DEG C temperature respectively by analysis for soybean powder, mealy potato and powder of straw nutrition powder carry out high temperature it is degerming and dry
Roasting water removal, continues 2.5h, makes analysis for soybean powder, mealy potato and the charing of powder of straw part, carbonization quality is than accounting for the 18% of gross mass, you can
Stand-by charcoal base analysis for soybean powder, mealy potato and powder of straw are obtained respectively；
2. 1. stand-by charcoal base analysis for soybean powder, mealy potato and powder of straw that step is obtained are by weight 1：1：2 ratio machinery
It is well mixed, obtain stand-by nutrition powder；
3. by bacillus licheniformis, Rhodopseudomonas palustris, thuringiensis, photosynthetic bacteria, bacillus subtilis
Each 5 parts of the stand-by nutrition powder that respectively 2. implantation step obtains, and encapsulation preserves respectively；
4. the nutrition powder for being implanted with Rhodopseudomonas palustris is placed under 35 DEG C of closed environment, 2.5 days are incubated；Will
The nutrition powder for being implanted with photosynthetic bacteria is placed under 23 DEG C of closed environment, is incubated 8 days；The battalion of bacillus licheniformis will be implanted with
Foster powder is placed under 31 DEG C of closed environment, is incubated 4.5 days；The nutrition powder for being implanted with bacillus subtilis is placed in 36 DEG C
Under environment, 3.5 days are incubated；In the environment of the nutrition powder for being implanted with thuringiensis is placed in into 36 DEG C, 2.5 days are incubated；
5. and then the nutrition powder that five kinds of preservations have objective microbe is all mixed simultaneously with remaining stand-by nutrition powder
Stir, obtain pending biomass carbon base soil-repairing agent；
6. 5. pending biomass carbon base soil-repairing agent that step is obtained is placed in lucifuge and divulged information, 22 DEG C of environment temperature,
6 days in the environment of humidity 60%, that is, obtain needed for biomass carbon base soil-repairing agent.
Embodiment 2
It is overall same as Example 1, it is in place of difference：
A kind of biomass carbon base soil-repairing agent, is made up of the composition of following parts by weight：Nutrition powder is by total weight parts 100
Part, weight ratio by 2: 2: 5 mixing analysis for soybean powder, mealy potato and powder of straw composition, preservation have objective microbe bacillus licheniformis,
Rhodopseudomonas palustris, thuringiensis, red germ (one kind of photosynthetic bacteria, while have and remove part sulphur in soil
Effect), bacillus subtilis.
The manufacture method of above-mentioned biomass carbon base soil-repairing agent, comprises the following steps：
1. using 150 DEG C temperature respectively by analysis for soybean powder, mealy potato and powder of straw nutrition powder carry out high temperature it is degerming and dry
Roasting water removal, continues 2h, makes analysis for soybean powder, mealy potato and the charing of powder of straw part, carbonization quality is than accounting for the 15% of gross mass, you can point
Stand-by charcoal base analysis for soybean powder, mealy potato and powder of straw are not obtained；
2. 1. stand-by charcoal base analysis for soybean powder, mealy potato and powder of straw that step is obtained are by weight 2: 2: 5 ratio machinery
It is well mixed, obtain stand-by nutrition powder；
4. the nutrition powder for being implanted with Rhodopseudomonas palustris is placed under 33 DEG C of closed environment, 2 days are incubated；Will implantation
The nutrition powder for having red germ is placed under 22 DEG C of closed environment, is incubated 7 days；The nutrition powder of bacillus licheniformis will be implanted with
It is placed under 30 DEG C of closed environment, is incubated 4 days；In the environment of the nutrition powder for being implanted with bacillus subtilis is placed in into 35 DEG C,
Insulation 3 days；In the environment of the nutrition powder for being implanted with thuringiensis is placed in into 35 DEG C, 2 days are incubated；
6. 5. pending biomass carbon base soil-repairing agent that step is obtained is placed in lucifuge and divulged information, 20 DEG C of environment temperature,
5 days in the environment of humidity 50%, that is, obtain needed for biomass carbon base soil-repairing agent.
Embodiment 3
It is overall same as Example 1, it is in place of difference：
A kind of biomass carbon base soil-repairing agent, it is characterised in that：It is made up of the composition of following parts by weight：Nutrition powder by
100 parts of total weight parts, weight ratio is by the analysis for soybean powder of 3: 3: 5 mixing, mealy potato and powder of straw composition, and preservation is with having objective microbe
Clothing bacillus, Rhodopseudomonas palustris, thuringiensis, photosynthetic bacteria, bacillus subtilis.
The manufacture method of above-mentioned biomass carbon base soil-repairing agent, comprises the following steps：
1. using 200 DEG C temperature respectively by analysis for soybean powder, mealy potato and powder of straw nutrition powder carry out high temperature it is degerming and dry
Roasting water removal, continues 3h, makes analysis for soybean powder, mealy potato and the charing of powder of straw part, carbonization quality is than accounting for the 20% of gross mass, you can point
Stand-by charcoal base analysis for soybean powder, mealy potato and powder of straw are not obtained；
2. 1. stand-by charcoal base analysis for soybean powder, mealy potato and powder of straw that step is obtained are by weight 3: 3: 5 ratio machinery
It is well mixed, obtain stand-by nutrition powder；
4. the nutrition powder for being implanted with Rhodopseudomonas palustris is placed under 38 DEG C of closed environment, 3 days are incubated；Will implantation
The nutrition powder for having photosynthetic bacteria is placed under 25 DEG C of closed environment, is incubated 10 days；The nutrition of bacillus licheniformis will be implanted with
Powder is placed under 32 DEG C of closed environment, is incubated 5 days；The nutrition powder for being implanted with bacillus subtilis is placed in 38 DEG C of environment
Under, it is incubated 4 days；In the environment of the nutrition powder for being implanted with thuringiensis is placed in into 38 DEG C, 3 days are incubated；
6. 5. pending biomass carbon base soil-repairing agent that step is obtained is placed in lucifuge and divulged information, 25 DEG C of environment temperature,
8 days in the environment of humidity 70%, that is, obtain needed for biomass carbon base soil-repairing agent.
The foregoing description of the disclosed embodiments, only for enabling professional and technical personnel in the field to realize or using this
Invention.A variety of modifications to these embodiments will be apparent for those skilled in the art, institute herein
The General Principle of definition can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore,
The present invention is not intended to be limited to the embodiments shown herein, and is to fit to special with principles disclosed herein and novelty
The consistent most wide scope of point.
Claims (2)
- A kind of 1. biomass carbon base soil-repairing agent, it is characterised in that：It is made up of the composition of following parts by weight：Nutrition powder is by total 100 parts of parts by weight, weight ratio have objective microbe by the analysis for soybean powder of 2-3: 2-3: 5 mixing, mealy potato and powder of straw composition, preservation Bacillus licheniformis, Rhodopseudomonas palustris, thuringiensis, photosynthetic bacteria, bacillus subtilis.
- 2. the manufacture method of biomass carbon base soil-repairing agent according to claim 1, it is characterised in that including following step Suddenly：1. using 150 DEG C -200 DEG C temperature respectively by analysis for soybean powder, mealy potato and powder of straw nutrition powder carry out high temperature it is degerming and Water removal is bakeed, continues 2h-3h, makes analysis for soybean powder, mealy potato and the charing of powder of straw part, 15%- of the carbonization quality than accounting for gross mass 20%, you can obtain stand-by charcoal base analysis for soybean powder, mealy potato and powder of straw respectively；It is 2. 1. stand-by charcoal base analysis for soybean powder, mealy potato and powder of straw that step obtains is mechanical by weight 2-3: 2-3: 5 ratio It is well mixed, obtain stand-by nutrition powder；3. bacillus licheniformis, Rhodopseudomonas palustris, thuringiensis, photosynthetic bacteria, bacillus subtilis are distinguished 2. each 5 parts of stand-by nutrition powder that implantation step obtains, and encapsulation preserves respectively；4. the nutrition powder for being implanted with Rhodopseudomonas palustris is placed under 33 DEG C -38 DEG C of closed environment, -3 days 2 days are incubated； The nutrition powder for being implanted with photosynthetic bacteria is placed under 22 DEG C -25 DEG C of closed environment, is incubated -10 days 7 days；Lichens will be implanted with The nutrition powder of bacillus is placed under 30 DEG C -32 DEG C of closed environment, is incubated -5 days 4 days；Bacillus subtilis will be implanted with Nutrition powder be placed in the environment of 35 DEG C -38 DEG C, be incubated -4 days 3 days；The nutrition powder of thuringiensis will be implanted with It is placed in the environment of 35 DEG C -38 DEG C, is incubated -3 days 2 days；5. and then the nutrition powder that five kinds of preservations have objective microbe is all mixed and stirred for remaining stand-by nutrition powder Uniformly, pending biomass carbon base soil-repairing agent is obtained；6. 5. pending biomass carbon base soil-repairing agent that step is obtained is placed in lucifuge and divulged information, 20 DEG C -25 DEG C of environment temperature, - 8 days 5 days in humidity 50%-70% environment, that is, obtain needed for biomass carbon base soil-repairing agent.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108311533A (en) * | 2018-05-16 | 2018-07-24 | 辽宁大学 | Utilize the method for heavy metal in biomass carbon-plant-microorganism joint rehabilitating soil |
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