CN108083914A - A kind of more effect composite microbic bacterial fertilizers and preparation method thereof - Google Patents
A kind of more effect composite microbic bacterial fertilizers and preparation method thereof Download PDFInfo
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- CN108083914A CN108083914A CN201711440969.0A CN201711440969A CN108083914A CN 108083914 A CN108083914 A CN 108083914A CN 201711440969 A CN201711440969 A CN 201711440969A CN 108083914 A CN108083914 A CN 108083914A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
- C05D3/02—Calcareous fertilisers from limestone, calcium carbonate, calcium hydrate, slaked lime, calcium oxide, waste calcium products
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of more effect composite microbic bacterial fertilizers, are mainly made of the raw material of following proportion by weight：Composite flora tunning 50~60, rhamnolipid 2~3, offal 9~11, oyster shell whiting 20~24 and sodium humate 9~12, the composite flora tunning are obtained by the way that composite flora to be carried out to gradient temperature fermentation in fermentation substrate.Composite flora tunning in the bacterial manure, using multiple-microorganism strain and suitable fermentation substrate, carry out gradient temperature fermentation, obtaining has synergistic effect, the metabolite species composite flora tunning high mostly with content, the composite flora tunning combines other active ingredients, and the composite microbic bacterial fertilizer of acquisition has multiple efficacies.Also disclose the preparation method of above-mentioned more effect composite microbic bacterial fertilizers.
Description
Technical field
The invention belongs to microbial manure technical fields, and in particular to a kind of more effect composite microbic bacterial fertilizers and its preparation
Method.
Background technology
In these years, agriculture chemical dosage in China's is quite big, is global maximum agriculture chemical producing country and using state, mistake
Degree relies on chemical fertilizer, a large amount of applying pesticides make land deterioration serious, and soil nutrient is uneven, soluble salt too high levels, soil
Secondary salinization, geobiont quantity are reduced, and structure of community changes, and beneficial organism quantity is reduced, and harmful poisonous substance increases,
Directly influence growth and the agricultural product quality of crop.Therefore, prevention and cure of pollution and restoration of the ecosystem are carried out to pollution and degenerated soil
The problem of in the urgent need to address in China's agricultural development is become.
Composite microbiological fertilizer refers to that specified microorganisms are combined with nutriment, can provide, keeps or improve plant
Nutrition improves agricultural output or improves the living microorganism product of quality of agricultural product.Microbial-bacterial fertilizer can be solved preferably
For a long time, the excessive adverse consequences applied chemical fertilizer and brought to soil, can improve soil texture, and the microorganism in culture soil increases
Strong soil fertility improves quality of agricultural product.
Composite microbial bacteria mainly has following effect：
First, plant growth is promoted
The beneficial microbes such as bacillus megaterium, bacillusmusilaginosiengineering, nitrogen-fixing bacteria generate largely in metabolic process
Metabolite, these metabolites can effectively improve absorption of the crop to nutrient, so as to reduce fertilizer application amount.
2nd, increasing both production and income
The metabolite of bacillusmusilaginosiengineering, bacillus laterosporus, bacillus licheniformis etc. beneficial bacterium can promote to make
Object root system development is mainly shown as that fibrous root quantity increases.It is metabolized aborning plant growth regulator to enter in crop body, can promote
It into crop photosynthesis, adjusts nutrient and flows to fruit, in the case where equivalence is put into, than the volume increase of application chemical fertilizer
15%-30%.With climbing to a higher point successively for fertilizer price, this advantage becomes apparent performance.
3rd, quality is improved
The beneficial microbes such as bacillus laterosporus, bacillus subtilis, bacillus coagulans can reduce nitric acid in plant
Salt content is up to more than 20%；Content of beary metal can be reduced；Vitamin C content in fruit is made to improve more than 30%, soluble sugar carries
High 2-4 degree；The content of essential amino acid in fruit, B family vitamin, unrighted acid can be improved.Fruit mouthfeel significantly improves,
Storage endurance more to fertilize.
4th, continuous cropping is prevented
The beneficial microbes such as rice aspergillus, bacillus licheniformis, bacillus subtilis can accelerate the decomposition of organic substance, to make
Object provides nutrient, equally can also decompose the poisonous and harmful substance of continuous cropping generation, prevent continuous cropping.
5th, rhizospheric environment is protected
After beneficial microbe is manured into soil, it is multiplied into rapidly
6th, it is dominant microflora to enhance resistance, controls rhizosphere nutrition and resource, and pathogen is made to lose living space and item
Part, a large amount of profitable strains, which are bred, to be formed dominant population and can effectively inhibit the breeding of harmful bacteria.Beneficial microbe can make crop root
The cell wall thickening of cell, fibrosis, lignifying, the double silicon layers of generation cutin effectively prevent the intrusion of pathogen.
The beneficial microbes such as bacillus licheniformis, bacillus megaterium, bacillus laterosporus can enhance buffering power of soil,
Water conservation moisturizing, enhancing crop drought resistance, cold-resistant, waterlogging-resistant ability；Bacillus laterosporus can also strengthen blade protection film simultaneously, resist disease
Opportunistic pathogen infects, disease-resistant, pest-resistant.
Composite microbial bacteria has the advantages that as above more in agricultural production, and many scholars develop for its advantage at present
Diversified composite microbic bacterial fertilizer.But various composite bacterial fertilizer technologies have shortcoming, are mainly shown as the following：
(1) strain is single.Mainly with to bacillus subtilis, bacillusmusilaginosiengineering, bacillus amyloliquefaciens, lactic acid bacteria,
Based on saccharomycete, carried out on the basis of more than strain compound.
(2) fermentation condition is fixed.Fermentation condition uses spontaneous fermentation, and such composite bacteria is difficult to act synergistically, microorganism
Metabolite content and species are less.
(3) fermentation substrate is based on farm manure, stalk.Using farm manure, stalk as fermentation substrate fertilizer efficiency relatively low, nutrient
Content is relatively low.
More than deficiency causes products obtained therefrom function weaker, although with certain diseases prevention, improving agricultural output and quality, thorn
Swash growth, the function of rehabilitating soil environment, but it acts on unobvious, it is limited to the effect of the current Agriculture Present Situation in China.
The content of the invention
It is an object of the invention to provide a kind of more effect composite microbic bacterial fertilizers, the composite flora fermentation in the bacterial manure is produced
Object using multiple-microorganism strain and suitable fermentation substrate, carries out gradient temperature fermentation, and obtaining has synergistic effect, metabolism
The product species composite flora tunning high mostly with content, the composite flora tunning combine other active ingredients, obtain
The composite microbic bacterial fertilizer obtained has multiple efficacies.
The present invention also aims to provide the preparation method of above-mentioned more effect composite microbic bacterial fertilizers.
Above-mentioned first purpose of the present invention is achieved through the following technical solutions：A kind of more effect complex microorganisms
Bacterial manure is mainly made of the raw material of following proportion by weight：Composite flora tunning 50~60, rhamnolipid 2~3, offal 9
~11, oyster shell whiting 20~24 and sodium humate 9~12, the composite flora tunning by by composite flora in fermentation substrate
Middle progress gradient temperature fermentation obtains.
The composite flora includes at least bacillus subtilis, photosynthetic bacteria, denitrifying bacteria, splits born of the same parents bacterium and side spore gemma
Bacillus, the composite flora are added in the form of microbial inoculum in the fermentation substrate, and dosage is the fermentation substrate gross mass
0.5~1%.
In more effect composite microbic bacterial fertilizers, the concentration of the bacillus subtilis is 7~1,000,000,000/g, described
The concentration of photosynthetic bacteria is 1~300,000,000/g, and the denitrifying bacteria concentration is 1~200,000,000/g, and described splits born of the same parents' bacteria concentration
For 1~200,000,000/g, the concentration of the bacillus laterosporus is 4~700,000,000/g.
The effect of these floras includes improvement soil microorganism species structure, improves soil environment, increases beneficial bacterium, reduces
Harmful bacteria is finally reached soil improvement, the effect of disease resistance.
In addition these probiotics flora amount reproductions can limit the breeding of soil harmful bacteria, and the secretion of these beneficial bacteriums
Metabolism has sterilization, inhibits the effect of harmful bacteria growth, can also secret out of the adjusting and controlling growth substance for stimulating plant growth, stimulates and plants
Object growth and development.
Fermentation substrate of the present invention is made of the raw material of following proportion by weight：Aloe 20~30, dregs of beans 140~160,
Rice bran 180~220, corn 180~220.
These fermentation substrates can provide nutrient and power for composite flora.
When gradient temperature ferments, initial temperature is 3~5 DEG C, and temperature raises gradient as 3~5 DEG C/day, fermentation time 10
~20 days, fermentation temperature scope was 5~70 DEG C.
When gradient temperature ferments, fermentation humidity is 50~70%.
Further, composite flora is carried out in fermentation substrate after gradient temperature fermentation again through naturally dry, crush and
It crosses the processing of 80 mesh sieves and obtains composite flora tunning.
Rhamnolipid in the present invention has rehabilitating soil crumb structure, improves soil fixed nitrogen, molybdenum, phosphorus, potassium capability, and
It can be enriched with, remove, the noxious material in degradation soil, the heavy metal ion that can be passivated or remove in sludge handles polycyclic aromatic hydrocarbon
With the toxicity for refining oily product etc., elimination or decrease environmental contaminants, deteriorating pesticide residue adjusts ecological environment of soil.
Offal in the present invention is common natural biological expelling parasite, insecticide, contains nicotine in offal, to various pests
With stomach toxicity, tag and fumigation action, poisoning mechanism is paralysis.Offal to subterranean pest-insect (grub, wireworm, maggot etc.) tool
There is preferable control effect.
Oyster shell whiting in the present invention, 95% main component is calcium carbonate, also has a small amount of amino acid and polysaccharide material, main
It is used to adjust soil pH, calcium source is provided for crop.
Sodium humate in the present invention, nickname natrium humate, rotten sodium, sodium humate, can be used as fertilizer and plant growth thorn
Hormone can stimulate crop growth, help the absorption of nutrient, improve soil texture, improve crop drought resistance ability, promote
Nitrogen-fixing bacteria activation.
Above-mentioned second purpose of the present invention is achieved through the following technical solutions：Above-mentioned more effect complex microorganisms
The preparation method of bacterial manure, comprises the following steps：
(1) composite flora is chosen, is placed in fermentation substrate, after gradient temperature ferments, obtains composite flora tunning；
(2) press and choose composite flora tunning, rhamnolipid, offal, oyster shell whiting and sodium humate with magnitude relation, mix
It is even to get more effect composite microbic bacterial fertilizers.
Compared with prior art, the invention has the advantages that：
(1) the composite bacteria agent species that composite flora tunning of the present invention uses are more, including at least withered grass gemma
Bacillus about, photosynthetic bacteria, denitrifying bacteria, split 5 kinds of born of the same parents bacterium and bacillus laterosporus etc.；
(2) mixing that fermentation substrate of the present invention is arranged in pairs or groups according to a certain percentage with aloe, dregs of beans, corn and rice bran, by multiple
The fermentation of flora temperature gradient is closed, gained tunning fertilizer efficiency is high, and nutritive element content is high, ferments under temperature gradient conditions, more
Kind microorganism species can finally reach synergistic effect, and metabolite species is mostly high with content, and fermenting substrate is thorough；
(3) offal is with the addition of in the raw material of the more effect composite microbic bacterial fertilizers of the present invention, the effect with expelling parasite or desinsection
Fruit, the rhanolipid as biosurfactant of addition can remove the poisonous and pernicious species in soil to soil phosphorus decomposing, potassium decomposing, blunt
Change heavy metal etc., its 95% ingredient of the oyster shell whiting of addition is calcium carbonate, can adjust soil pH, solve the problems, such as soil acidification, add
The sodium humate added can stimulate crop growth, help the absorption of nutrient, improve soil texture, improve crop drought resistance energy
Power, promotes nitrogen-fixing bacteria activation, and the present invention obtains these ingredients with difference in functionality with more work(by reasonably combined
The product of energy；
(4) more effect composite microbic bacterial fertilizers in the present invention have increasing both production and income, improve quality, insect-repelling and disease-proof, improvement
Soil pH removes soil poisonous and harmful substance, heavy metal-passivated, degrading pesticide residues, improves crop anti-adversity and other effects；
(5) more effect composite microbic bacterial fertilizers in the present invention are nontoxic, harmless, degradable, and products obtained therefrom is environmentally protective, ring
Border is friendly, and strain quantity is more, viable bacteria Particle density is high, and upgrading synergistic effect is apparent, and expelling parasite, protection effect are good, can be obviously improved soil
Earth pH, can effectively remove soil poisonous and harmful substance, the heavy metal that can be effectively passivated in soil, can effectively degrade soil and farming
The pesticide residue on object surface effectively improves crop anti-adversity；
(6) more effect composite microbic bacterial fertilizers increasing that subtracts one to China's agricultural two in the present invention, agriculture green production, reduce
Agricultural non-point source pollution plays an important roll, and significant contribution is made for the sustainable development of China's agricultural.
Specific embodiment
Embodiment 1
More effect composite microbic bacterial fertilizers provided in this embodiment, are mainly made of the raw material of following proportion by weight：It is multiple
It closes microflora fermentation product 50, rhamnolipid 2, offal 9, oyster shell whiting 20 and sodium humate 9, composite flora tunning and passes through compound
Flora carries out gradient temperature fermentation in fermentation substrate and obtains.
Composite flora includes at least bacillus subtilis, photosynthetic bacteria, denitrifying bacteria, splits born of the same parents bacterium and side spore gemma bar
Bacterium, the composite flora are added in the form of microbial inoculum in the fermentation substrate, and dosage is the 0.5% of fermentation substrate gross mass.
In more effect composite microbic bacterial fertilizers, the concentration of bacillus subtilis is 7~1,000,000,000/g, photosynthetic bacteria it is dense
It spends for 1~300,000,000/g, denitrifying bacteria concentration is 1~200,000,000/g, splits born of the same parents' bacteria concentration for 1~200,000,000/g, the side spore bud
The concentration of spore bacillus is 4~700,000,000/g.
Fermentation substrate is made of the raw material of following proportion by weight：Aloe 20, dregs of beans 160, rice bran 180, corn 220.
When gradient temperature ferments, initial temperature is 3 DEG C, and temperature rise gradient is 3 DEG C/day, and fermentation time is 20 days, fermentation
Temperature range is 3~63 DEG C.
When gradient temperature ferments, fermentation humidity is 50~70%.
Composite flora is handled after gradient temperature fermentation is carried out in fermentation substrate through naturally dry, crushing and 80 mesh sieves of mistake again
Obtain composite flora tunning.
The preparation method of above-mentioned more effect composite microbic bacterial fertilizers, comprises the following steps：
(1) composite flora is chosen, is placed in fermentation substrate, after gradient temperature ferments, obtains composite flora tunning；
(2) press and choose composite flora tunning, rhamnolipid, offal, oyster shell whiting and sodium humate with magnitude relation, mix
It is even to get more effect composite microbic bacterial fertilizers.
The present embodiment obtain more effect composite microbic bacterial fertilizers can carry out metering packing, after processing by different weight into
Row packaging, can be respectively 50g/ bags, 100g/ bags, 250g/ bags, 500g/ bags, 1000g/ bags, 2500g/ bags.
Embodiment 2
More effect composite microbic bacterial fertilizers provided in this embodiment, are mainly made of the raw material of following proportion by weight：It is multiple
Microflora fermentation product 55, rhamnolipid 2.5, offal 10, oyster shell whiting 22 and sodium humate 11, composite flora tunning is closed to pass through
Composite flora carries out gradient temperature fermentation in fermentation substrate and obtains.
Composite flora includes at least bacillus subtilis, photosynthetic bacteria, denitrifying bacteria, splits born of the same parents bacterium and bacillus laterosporus
This 5 floras, the composite flora are added in the form of microbial inoculum in the fermentation substrate, and dosage is the total matter of fermentation substrate
The 0.8% of amount.
In more effect composite microbic bacterial fertilizers, the concentration of bacillus subtilis is 7~1,000,000,000/g, photosynthetic bacteria it is dense
It spends for 1~300,000,000/g, denitrifying bacteria concentration is 1~200,000,000/g, splits born of the same parents' bacteria concentration for 1~200,000,000/g, the side spore bud
The concentration of spore bacillus is 4~700,000,000/g.
Fermentation substrate is made of the raw material of following proportion by weight：Aloe 25, dregs of beans 150, rice bran 200, corn 200.
When gradient temperature ferments, initial temperature is 5 DEG C, and temperature rise gradient is 5 DEG C/day, and fermentation time is 13 days, fermentation
Temperature range is 5~70 DEG C.
When gradient temperature ferments, fermentation humidity is 50~70%.
Composite flora is handled after gradient temperature fermentation is carried out in fermentation substrate through naturally dry, crushing and 80 mesh sieves of mistake again
Obtain composite flora tunning.
The preparation method of above-mentioned more effect composite microbic bacterial fertilizers is the same as embodiment 1.
Embodiment 3
More effect composite microbic bacterial fertilizers provided in this embodiment, are mainly made of the raw material of following proportion by weight：
Composite flora tunning 60, rhamnolipid 3, offal 11, oyster shell whiting 24 and sodium humate 12, composite flora fermentation
Product carries out gradient temperature fermentation in fermentation substrate by composite flora and obtains.
Composite flora includes at least bacillus subtilis, photosynthetic bacteria, denitrifying bacteria, splits born of the same parents bacterium and side spore gemma bar
Bacterium, the composite flora are added in the form of microbial inoculum in the fermentation substrate, and dosage is the 1.0% of fermentation substrate gross mass.
In more effect composite microbic bacterial fertilizers, the concentration of bacillus subtilis is 7~1,000,000,000/g, photosynthetic bacteria it is dense
It spends for 1~300,000,000/g, denitrifying bacteria concentration is 1~200,000,000/g, splits born of the same parents' bacteria concentration for 1~200,000,000/g, the side spore bud
The concentration of spore bacillus is 4~700,000,000/g.
Fermentation substrate is made of the raw material of following proportion by weight：Aloe 30, dregs of beans 140, rice bran 220, corn 180.
When gradient temperature ferments, initial temperature is 4 DEG C, and temperature rise gradient is 4 DEG C/day, and fermentation time is 16 days, fermentation
Temperature range is 4~68 DEG C.
When gradient temperature ferments, fermentation humidity is 50~70%.
Composite flora is handled after gradient temperature fermentation is carried out in fermentation substrate through naturally dry, crushing and 80 mesh sieves of mistake again
Obtain composite flora tunning.
The preparation method of above-mentioned more effect composite microbic bacterial fertilizers is the same as embodiment 1.
Embodiment 4
Test site：Haikou City, Hainan Province Longhua District Gao Po villages
Test material：Roll up core white
Experiment kind design：Totally two pieces of ground, every piece of ground are divided into 12 growing areas, altogether three batches of core white of plantation volume, such as the following table 1：
It more effect composite microbic bacterial fertilizers and its is used simultaneously with chemical fertilizer and blank pair in 1 common fertilizer of table, embodiment 2
According to situation
Conclusion (of pressure testing)：Product of the present invention is applied in trial zone 4,5,6, through measuring its Weight per plant, plant height, root weight, root long, aqueous
Rate, Chlorophyll, downy mildew, knee are above other experimental plots, wherein 4 yield highest of trial zone, while pest and disease damage quantity
It reduces, soil beneficial bacterium quantity increases, and harmful bacteria quantity is reduced.10 can be reduced using fertilizer and pesticide amount of application after product of the present invention
~30%.Likewise, embodiment 1 also has similar effect with the product in 3, no longer repeat one by one herein.
2 different situations last volume core white growing state of table
Embodiment 5
Test site：Hainan Province Danzhou City
Test material：Rice
Experimental design as different from Example 4, using more effect composite microbic bacterial fertilizers in embodiment 3, tie by experiment
Fruit is as shown in table 3 below：Substantially increase production compared with contrast ratio using product of the present invention, per mu yield, mass of 1000 kernel, tiller number, spike length, grain number per spike
It is above control group.Fertilizer and pesticide amount of application can reduce 10~30%.Likewise, the product in Examples 1 and 2 also have it is similar
Effect no longer repeats one by one herein.
Paddy growth situation under 3 different situations of table
| Experimental plot | Per mu yield (g) | Mass of 1000 kernel | Spike length | Grain number per spike | Tiller number |
| 1 | 354611.7 | 17.20 | 23.1 | 154 | 21 |
| 2 | 351921.1 | 17.72 | 22.9 | 148 | 23 |
| 3 | 334871.5 | 17.67 | 22.7 | 149 | 21 |
| 4 | 446959.3 | 18.29 | 24.5 | 155 | 25 |
| 5 | 432793.6 | 18.94 | 23.7 | 151 | 24 |
| 6 | 421674.7 | 18.67 | 23.4 | 150 | 24 |
| 7 | 339067.3 | 17.10 | 23.9 | 132 | 23 |
| 8 | 334278.8 | 17.86 | 22.6 | 132 | 25 |
| 9 | 320915.5 | 17.36 | 22.1 | 133 | 23 |
| 11 | 295770.5 | 17.15 | 20.4 | 128 | 18 |
| 12 | 264108.9 | 17.8 | 21.6 | 102 | 19 |
| 13 | 263343.2 | 16.59 | 20.8 | 128 | 17 |
Two above case illustrates that the microbial-bacterial fertilizer in the present invention is respectively provided with raising crop yield, reduces fertilizer and pesticide
Dosage reduces the multifunctional comprehensive effect of pest and disease damage.
It will be apparent to those skilled in the art that technical solution that can be as described above and design, make other various
Corresponding change and deformation, and all these changes and deformation should all belong to the protection domain of the claims in the present invention
Within.
Claims (8)
1. a kind of more effect composite microbic bacterial fertilizers, it is characterized in that being mainly made of the raw material of following proportion by weight：Compound bacteria
Group's tunning 50~60, rhamnolipid 2~3, offal 9~11, oyster shell whiting 20~24 and sodium humate 9~12, it is described compound
Microflora fermentation product is obtained by the way that composite flora to be carried out to gradient temperature fermentation in fermentation substrate.
2. more effect composite microbic bacterial fertilizers according to claim 1, it is characterized in that：The composite flora includes at least
Bacillus subtilis, photosynthetic bacteria, split born of the same parents bacterium and bacillus laterosporus at denitrifying bacteria, and the composite flora is with the shape of microbial inoculum
Formula is added in the fermentation substrate, and dosage is the 0.5~1% of the fermentation substrate gross mass.
3. more effect composite microbic bacterial fertilizers according to claim 2, it is characterized in that：In more effect composite microbic bacterial fertilizers
In, the concentration of the bacillus subtilis is 7~1,000,000,000/g, and the concentration of the photosynthetic bacteria is 1~300,000,000/g, institute
The denitrifying bacteria concentration stated is 1~200,000,000/g, and described splits born of the same parents' bacteria concentration for 1~200,000,000/g, the side spore gemma bar
The concentration of bacterium is 4~700,000,000/g.
4. more effect composite microbic bacterial fertilizers according to claim 1, it is characterized in that：The fermentation substrate is by following matter
Measure the raw material composition of part proportioning：Aloe 20~30, dregs of beans 140~160, rice bran 180~220, corn 180~220.
5. more effect composite microbic bacterial fertilizers according to claim 1, it is characterized in that：When gradient temperature ferments, initial temperature
It spends for 3~5 DEG C, temperature rise gradient is 3~5 DEG C/day, and fermentation time is 10~20 days, and fermentation temperature scope is 5~70 DEG C.
6. more effect composite microbic bacterial fertilizers according to claim 1, it is characterized in that：When gradient temperature ferments, fermentation is wet
It spends for 50~70%.
7. more effect composite microbic bacterial fertilizers according to claim 1, it is characterized in that：By composite flora in fermentation substrate
Carry out gradient temperature fermentation after again through naturally dry, crushing and cross 80 mesh sieves processing i.e. obtain composite flora tunning.
8. the preparation method of the more effect composite microbic bacterial fertilizers of claim 1-7 any one of them, it is characterized in that including following
Step：
(1) composite flora is chosen, is placed in fermentation substrate, after gradient temperature ferments, obtains composite flora tunning；
(2) press and choose composite flora tunning, rhamnolipid, offal, oyster shell whiting and sodium humate with magnitude relation, mixing, i.e.,
Much effect composite microbic bacterial fertilizers.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108794185A (en) * | 2018-07-20 | 2018-11-13 | 陈昱齐 | Application of the rhamnolipid as nitrification inhibitor |
| CN114317381A (en) * | 2022-03-04 | 2022-04-12 | 东北农业大学 | T-level microbial composite inoculant and preparation method and application thereof |
| CN115044495A (en) * | 2022-04-25 | 2022-09-13 | 西北工业大学深圳研究院 | Composite microbial remediation microbial inoculum for antibiotic-contaminated soil as well as preparation method and application thereof |
| CN116855430A (en) * | 2023-09-05 | 2023-10-10 | 中国农业科学院农业环境与可持续发展研究所 | Composite microbial agent for promoting corrosion and increasing yield of rice field straw and application thereof |
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| CN103483027A (en) * | 2013-09-23 | 2014-01-01 | 山东佐田氏生物科技有限公司 | Production method for composite bacterial fertilizer |
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| CN105601347A (en) * | 2015-08-19 | 2016-05-25 | 范明月 | Preparation method for crop organic fertilizer |
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| CN116855430A (en) * | 2023-09-05 | 2023-10-10 | 中国农业科学院农业环境与可持续发展研究所 | Composite microbial agent for promoting corrosion and increasing yield of rice field straw and application thereof |
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