CN107475161B - Process for removing hydrogen sulfide pollutants in household garbage by using microbial preparation - Google Patents
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
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Abstract
The invention belongs to the technical field of microorganisms, and discloses a process for removing hydrogen sulfide pollutants in household garbage by using a microbial preparation, which comprises the following steps: adding the microbial preparation into water with the weight of 10 times, and uniformly stirring to obtain a diluent; spreading the garbage into 50cm thick, inserting holes in the garbage, wherein the hole diameter is 1-5mm, and the hole density is 100-2Then in terms of per m2The diluent is uniformly sprayed by using 3-5kg of diluent, and the treatment time is more than 24 hours. The invention has simple and feasible process, environmental protection and no pollution.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a process for removing hydrogen sulfide pollutants in household garbage by using a microbial preparation.
Background
With the development of economy and the increase in the living standard of people, malodors have been receiving increasing attention as one of environmental hazards. In sewage treatment plants and in many industrial, agricultural and domestic waste dumps, a large amount of foul-smelling waste gases are produced, the main odoriferous component of which is sulphur-containing compounds. These substances have wide sources and extremely high toxicity, and among them, hydrogen sulfide is the most common and most widely affected.
The hydrogen sulfide removal methods are numerous and can be simply divided into wet desulfurization, dry desulfurization and biological desulfurization. The wet desulfurization is to remove the sulfur by the reaction of specific solvents such as sodium hydroxide, ammonia water and the like with hydrogen sulfideA method of hydrogen recycle is achieved by the action of oxygen on the solvent. Because of the influence of the flow velocity and the flow quantity of the sodium hydroxide, the hydrogen sulfide can not be completely dissolved in the sodium hydroxide, and thiosulfate can be generated in the dissolving process, which can influence the desulfurization effect and also has the problems of high investment, complex operation management, high desulfurization cost, replacement of absorption liquid and the like. Dry desulfurization is a desulfurization method in which oxygen is used and iron oxide is used as an oxidant to oxidize hydrogen sulfide into elemental sulfur or sulfide. Elemental sulfur plays a catalytic role in the absorption process. However, the dry desulfurization technology has the problems of large occupied area of devices, discontinuous operation, difficult regeneration and replacement of the desulfurizer, low desulfurization efficiency and the like. The biological desulfurization technique is to remove H through a microbial metabolic pathway2The removal technology for converting S into sulfate or elemental sulfur has the advantages of low operation cost, less secondary pollution and the like, and becomes a mainstream method for the research and application of malodorous prevention at home and abroad. However, in the prior art, microbial inoculum with reasonable compatibility and good effect is difficult to find, and most of microbial inoculum can not be symbiotically cooperated, so that the deodorization effect is poor and the requirements of people can not be met.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide the process for removing the hydrogen sulfide pollutants in the household garbage by using the microbial preparation, and the process has good treatment effect, is environment-friendly and has no pollution.
The above purpose of the invention is realized by the following technical scheme:
the process for removing the hydrogen sulfide pollutants in the household garbage by utilizing the microbial preparation comprises the following steps: adding the microbial preparation into water with the weight of 10 times, and uniformly stirring to obtain a diluent; spreading the garbage into 50cm thick, inserting holes in the garbage, wherein the hole diameter is 1-5mm, and the hole density is 100-2Then in terms of per m2The diluent is uniformly sprayed by using 3-5kg of diluent, and the treatment time is more than 24 hours.
In particular, the amount of the solvent to be used,
the preparation method of the microbial preparation comprises the following steps:
step 1) preparing a domestication culture medium, step 2) performing strain domestication, step 3) preparing a carrier, and step 4) preparing a microbial preparation.
Further, the air conditioner is provided with a fan,
the microbial preparation is prepared according to the following steps:
step 1) preparing an acclimatization culture medium: adding 50g of glucose, 20g of soybean meal, 10g of wheat bran, 5g of ammonium sulfate, 5g of sodium sulfide, 1g of dipotassium hydrogen phosphate, 1g of monopotassium phosphate and 1g of ferrous sulfate into water, and fixing the volume to 1L to prepare a domestication culture medium;
step 2) strain domestication: respectively culturing Burkholderia cepacia, enterococcus faecalis, Acidithiobacillus ferrooxidans, Candida albicans, Pseudomonas fluorescens and moist Cellulomonas cellulosae to obtain 1 × 108Mixing the CFU/ml seed solution according to the volume ratio of 2:3:3:5:5:7 to obtain a mixed seed solution, transferring the mixed seed solution into an acclimatization culture medium according to the inoculation amount of 8%, and performing acclimatization culture at 28 ℃ for 12 hours to obtain an acclimatization bacterial solution;
step 3) preparing a microbial carrier: uniformly mixing sawdust, manioc waste and turfy soil according to the mass ratio of 5:5:3 to obtain the fertilizer;
step 4) preparing a microbial preparation: adding the domesticated bacterial liquid into a microbial carrier with the mass 2 times that of the domesticated bacterial liquid, stirring at 200rpm for 3min, then drying at a low temperature of 20 ℃, and packaging after drying, wherein the water content is 6 wt%.
Preferably, the first and second electrodes are formed of a metal,
the Burkholderia cepacia is ATCC 25416; the enterococcus faecalis is ATCC 29212; said Acidithiobacillus ferrooxidans is ATCC 53993; the candida albicans is ATCC 10231; the pseudomonas fluorescens is ATCC 13525; the moist Cellulomonas was ATCC 491.
Further, the air conditioner is provided with a fan,
the foregoing are only preferred embodiments of the present invention. As a less preferred technical solution, the present invention also has no particular limitation on the number of strains in the seed liquid, which may be determined according to the environmental requirements.
The strains of the present invention belong to known strains and can be purchased from ATCC and other commercial sources. The seed culture and fermentation culture of each strain of the invention are conventional culture methods in the field, are not innovative points of the invention and are not detailed here. The starting materials or reagents used in the present invention are commercially available unless otherwise specified.
Compared with the prior art, the invention has the advantages that the following aspects are mainly included but not limited:
the process of the invention uses microbial preparation, is environment-friendly, pollution-free and low in cost, can effectively treat sulfur-containing malodorous substances such as hydrogen sulfide and the like, has good effect, and can be applied to garbage deodorization, methane and removal of hydrogen sulfide in oil wells;
the microbial preparation has the advantages that the strains are reasonably compatible, symbiotic and harmonious, and are not antagonistic, and the activity is high; the bacteria liquid is firstly domesticated and cultured, so that the bacteria liquid can adapt to the environment more quickly, and the desulfurization efficiency is improved;
according to the invention, a large amount of waste is used during preparation of the carrier, so that the cost is saved, and the survival time and the planting capacity of the strain can be enhanced; wherein, the sawdust and the cassava residue can adjust the water content and the air permeability of the soil, and can also provide nutrients and the turfy soil for water retention and ventilation; the composite microbial inoculum is prepared from a carrier and a mixed bacterial liquid, has good thallus attachment effect, large loading capacity and large specific surface area, and can improve the degradation speed;
the microbial preparation has the advantages of simple preparation process, low cost, good deodorization effect, ecology, environmental protection, convenient use, low production cost, no secondary pollution to the environment and wide application prospect.
Drawings
FIG. 1: the removal rate of hydrogen sulfide and odor after 24-hour treatment by the microbial preparation;
FIG. 2: and (3) treating the microorganism preparation for 48 hours to remove hydrogen sulfide and odor.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present application, the technical solutions in the present application will be clearly and completely described below with reference to specific embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The process for removing the hydrogen sulfide pollutants in the household garbage by utilizing the microbial preparation comprises the following steps: adding the microbial preparation into water with the weight of 10 times, and uniformly stirring to obtain a diluent; spreading the garbage into 50cm thick, inserting holes in the garbage, wherein the hole diameter is 2mm, and the hole density is 200/m2Then in terms of per m2The dilutions were uniformly sprayed with an amount of 3kg of dilution and the treatment time was 24 hours.
The preparation method of the microbial preparation comprises the following steps:
step 1) preparing an acclimatization culture medium: adding 50g of glucose, 20g of soybean meal, 10g of wheat bran, 5g of ammonium sulfate, 5g of sodium sulfide, 1g of dipotassium hydrogen phosphate, 1g of monopotassium phosphate and 1g of ferrous sulfate into water, and fixing the volume to 1L to prepare a domestication culture medium;
step 2) strain domestication: respectively culturing Burkholderia cepacia, enterococcus faecalis, Acidithiobacillus ferrooxidans, Candida albicans, Pseudomonas fluorescens and moist Cellulomonas cellulosae to obtain 1 × 108Mixing the CFU/ml seed solution according to the volume ratio of 2:3:3:5:5:7 to obtain a mixed seed solution, transferring the mixed seed solution into an acclimatization culture medium according to the inoculation amount of 8%, and performing acclimatization culture at 28 ℃ for 12 hours to obtain an acclimatization bacterial solution;
step 3) preparing a microbial carrier: uniformly mixing sawdust, manioc waste and turfy soil according to the mass ratio of 5:5:3 to obtain the fertilizer;
step 4) preparing a microbial preparation: adding the domesticated bacterial liquid into a microbial carrier with the mass 2 times that of the domesticated bacterial liquid, stirring at 200rpm for 3min, then drying at a low temperature of 20 ℃, and packaging after drying, wherein the water content is 6 wt%.
The Burkholderia cepacia is ATCC 25416; the enterococcus faecalis is ATCC 29212; said Acidithiobacillus ferrooxidans is ATCC 53993; the candida albicans is ATCC 10231; the pseudomonas fluorescens is ATCC 13525; the moist Cellulomonas was ATCC 491.
Example 2
The process for removing the hydrogen sulfide pollutants in the household garbage by utilizing the microbial preparation comprises the following steps: adding the microbial preparation into water with the weight of 10 times, and uniformly stirring to obtain a diluent; spreading the garbage into 50cm thick, inserting holes in the garbage, wherein the hole diameter is 4mm, and the hole density is 100/m2Then in terms of per m2The dilutions were uniformly sprayed with an amount of 4kg of dilution and the treatment time was 48 hours.
The preparation method of the microbial preparation comprises the following steps:
step 1) preparing an acclimatization culture medium: adding 50g of glucose, 20g of soybean meal, 10g of wheat bran, 5g of ammonium sulfate, 5g of sodium sulfide, 1g of dipotassium hydrogen phosphate, 1g of monopotassium phosphate and 1g of ferrous sulfate into water, and fixing the volume to 1L to prepare a domestication culture medium;
step 2) strain domestication: respectively culturing Burkholderia cepacia, enterococcus faecalis, Acidithiobacillus ferrooxidans, Candida albicans, Pseudomonas fluorescens and moist Cellulomonas cellulosae to obtain 1 × 108Mixing the CFU/ml seed solution according to the volume ratio of 2:3:3:5:5:7 to obtain a mixed seed solution, transferring the mixed seed solution into an acclimatization culture medium according to the inoculation amount of 8%, and performing acclimatization culture at 28 ℃ for 12 hours to obtain an acclimatization bacterial solution;
step 3) preparing a microbial carrier: uniformly mixing sawdust, manioc waste and turfy soil according to the mass ratio of 5:5:3 to obtain the fertilizer;
step 4) preparing a microbial preparation: adding the domesticated bacterial liquid into a microbial carrier with the mass 2 times that of the domesticated bacterial liquid, stirring at 200rpm for 3min, then drying at a low temperature of 20 ℃, and packaging after drying, wherein the water content is 6 wt%.
The Burkholderia cepacia is ATCC 25416; the enterococcus faecalis is ATCC 29212; said Acidithiobacillus ferrooxidans is ATCC 53993; the candida albicans is ATCC 10231; the pseudomonas fluorescens is ATCC 13525; the moist Cellulomonas was ATCC 491.
Example 3
The removal effect of hydrogen sulfide is detected in a laboratory stage:
firstly, 50ml of liquid culture medium (the formula is the same as the domestication culture medium, but sodium sulfide is not added) is placed in a 250ml culture bottle, the microbial preparation in the example 1 is diluted by adding 10 times of water by weight, then the microbial preparation is inoculated into the liquid culture medium according to the inoculation amount of 10%, 25ml of pure hydrogen sulfide gas is introduced, and the concentration of the hydrogen sulfide is detected in 24h and 48h respectively; a blank control (no microbial preparation added) is set at the same time; determination of the concentration of hydrogen sulfide (g/m) by methylene blue spectrophotometry3) The specific detection results are shown in table 1:
TABLE 1
| Group of | Initial concentration | 24h | 48h |
| Example 1 | 3.982 | 0.763 | 0.018 |
| Blank control | 4.017 | 3.914 | 3.875 |
And (4) conclusion: laboratory experiments show that the microbial preparation can effectively absorb and remove hydrogen sulfide gas, and the removal rate in 48 hours reaches more than 99%.
Example 4
The domestic garbage treatment effect test:
averagely dividing 8 tons of rotten household garbage into 8 parts, wherein each part is 1 ton, and the 8 parts are respectively placed in 8 closed rooms with the same size, namely an experimental group (an example 1 group) and a carrier control group (only a carrier is adopted and no microbial inoculum is added), and the rest is the same as the example 1; control group 1: the procedure of example 1 was repeated without adding Burkholderia cepacia; control group 2: the procedure of example 1 was repeated except that enterococcus faecalis was not added; control group 3: the same procedure as in example 1 was repeated except that Acidithiobacillus ferrooxidans was not added; control group 4: the procedure of example 1 was repeated except that Candida albicans was not added; control group 5: the procedure of example 1 was repeated except that Pseudomonas fluorescens was not added; control group 6: the same procedure as in example 1 was repeated except that moist Cellulomonas was not added;
the treatment method comprises the following steps: with reference to the method of example 1, H was determined by sampling at different collection points around the waste for 24H, 48H, respectively2S removal rate and odor removal rate. H2And S adopts an ammonium polyvinyl alcohol phosphate absorption-methylene blue colorimetric method. The odor concentration was measured using an XP-329IIIR odor concentration detector.
And (4) analyzing results: as shown in FIG. 1, the treatment time was 24 hours, test group H2The removal rate of S and odor is about 80%, while the removal rate of the carrier control group is only about 10%, and the treatment effect of the test group is obviously better than that of the carrier control group and is also better than that of the control groups 1-6; as shown in FIG. 2, the treatment time was 48H, test group H2The removal rate of S and odor is more than 90%, the treatment effect is obviously better than that of a control group, and the microbial preparation provided by the invention is reasonable in compatibility and good in synergistic effect.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (1)
1. ForThe process for removing the hydrogen sulfide pollutants in the household garbage by utilizing the microbial preparation comprises the following steps: adding the microbial preparation into water with the weight of 10 times, and uniformly stirring to obtain a diluent; spreading the garbage into 50cm thick, inserting holes in the garbage, wherein the hole diameter is 1-5mm, and the hole density is 100-2Then in terms of per m2Uniformly spraying the diluent with the amount of 3-5kg of diluent, wherein the treatment time is more than 24 hours;
the preparation method of the microbial preparation comprises the following steps: step 1) preparing a domestication culture medium, step 2) performing strain domestication, step 3) preparing a carrier, and step 4) preparing a microbial preparation;
the step 1) of preparing the domestication culture medium comprises the following steps: adding 50g of glucose, 20g of soybean meal, 10g of wheat bran, 5g of ammonium sulfate, 5g of sodium sulfide, 1g of dipotassium hydrogen phosphate, 1g of monopotassium phosphate and 1g of ferrous sulfate into water, and fixing the volume to 1L to prepare a domestication culture medium;
the step 2) of strain domestication comprises the following steps: respectively culturing Burkholderia cepacia, enterococcus faecalis, Acidithiobacillus ferrooxidans, Candida albicans, Pseudomonas fluorescens and moist Cellulomonas cellulosae to obtain 1 × 108Mixing the CFU/ml seed solution according to the volume ratio of 2:3:3:5:5:7 to obtain a mixed seed solution, transferring the mixed seed solution into an acclimatization culture medium according to the inoculation amount of 8%, and performing acclimatization culture at 28 ℃ for 12 hours to obtain an acclimatization bacterial solution; the step 3) for preparing the microbial carrier comprises the following steps: uniformly mixing sawdust, manioc waste and turfy soil according to the mass ratio of 5:5:3 to obtain the fertilizer;
the step 4) of preparing the microbial preparation comprises the following steps: adding the domesticated bacterial liquid into a microbial carrier with the mass 2 times that of the domesticated bacterial liquid, stirring at 200rpm for 3min, then drying at a low temperature of 20 ℃, and packaging after drying, wherein the water content is 6 wt%;
the Burkholderia cepacia is ATCC 25416; the enterococcus faecalis is ATCC 29212; said Acidithiobacillus ferrooxidans is ATCC 53993; the candida albicans is ATCC 10231; the pseudomonas fluorescens is ATCC 13525; the moist Cellulomonas was ATCC 491.
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