CN107467674A - Compound based on duckbilled fish active peptides - Google Patents
Compound based on duckbilled fish active peptides Download PDFInfo
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- CN107467674A CN107467674A CN201710480975.2A CN201710480975A CN107467674A CN 107467674 A CN107467674 A CN 107467674A CN 201710480975 A CN201710480975 A CN 201710480975A CN 107467674 A CN107467674 A CN 107467674A
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Classifications
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- C—CHEMISTRY; METALLURGY
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- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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Abstract
The invention discloses the compound based on duckbilled fish active peptides, the composition and its parts by weight of compound are：24 parts of duckbilled fish active peptides, 0.2 0.4 parts of vitamin B1,0.1 0.3 parts of vitamin B2,18 24 parts of black sesame powder, 22 28 parts of black bean powder, 8 12 parts of soyabean protein powder, 59 parts of skimmed milk power, 23 parts of sea mustard powder, 48 parts of codonopsis pilosula powder, 0.5 0.8 parts of ginger powder, 5 10 parts of white granulated sugar.The preparation method of the compound includes pretreatment, fluid nutrient medium preparation, seed liquor preparation, fermentation medium preparation, fermentation, purifying, compound preparation.Have the beneficial effect that：Compound of the present invention is safe and effective, and the raw material used are all food common in daily life, cheap, and regulation condition can have both been reached by diet, has promoted hair follicle growth, new hair has been grown, improves the purpose of white hair.
Description
Technical field
The present invention relates to fish products deep process technology field, more particularly, to the compounding based on duckbilled fish active peptides
Thing.
Background technology
Duckbilled fish（Polyodonspathala）, scientific name " U.S. paddlefish ", U.S. Mississippi Valley is originated in, is one
The large-scale economic freshwater fish of kind, category Gadiformes Shi Wen paddlefishes section.Be before 300,000,000 years (i.e. dinosaur period) just give birth on earth
It is living, and a kind of rare sturgeons remained, it is one of only two kinds in Polyodontidae in the world.It and mandarin sturgeon
And the paddlefish in Water area of Changjiang River belongs to Gadiformes paddlefish section together.Duckbilled fish (paddlefish) is cartilaginous fish, and body surface is exposed, pool profit nothing
Squama, kiss is in soupspoon shape, likeness in form duckbilled, therefore also known as duckbilled sturgeon.Its kiss is especially long, in flat, such as pulpous state;Body surface smooth without squama,
The black pewter in back, often have between some spots wherein；There are point-like reddish brown, belly white in side;The big eye of mouth is small, and forehead is higher than mouth
Portion；Gill raker is close and elongated, and nucleus is big and is extended down to abdomeinal fin backward, and tail fin bifurcated, caudal peduncle drapes over one's shoulders the first squama of pedicel shape.Paddlefish
Individual is larger, and the life-span is more than 30 years.The sexual gland maturation of paddlefish is later, typically will be in the above ability sexal maturity of 8 ages.Paddlefish
Feeding habits are using the zooplankter that ingests as bait, are filter-feeding fishes similar to the bighead in China.Duckbilled fish whole body is all precious, duck
Mouth fish has medicine healthy sofa effect, to ensure the nutrition of duckbilled fish rich in having abundant glue protein, multivitamin and amino acid
Obtain best reservation and fully absorbed by human body, it is just very fastidious that it cooks meals requirement.Duckbilled fish cooks meals, exactly slow with warm fire
The method boiled, the most fresh vegetables of collocation, ensure the nutrition at utmost retaining duckbilled fish.Duckbilled fish is few into flesh of fish thorniness, duck
The fishbone of mouth fish can be swallowed after biting into pieces, be truly realized the peculiar phenomenon of " eat fish and do not tell bone ".Duckbilled fish matter is carefully delicious, and category is high
Albumen low fat meat.Fish-egg is processed into caviar and is referred to as black gold bullion, therefore, has very high edibility.In addition, spoon
The medical value for kissing sturgeon is high.Modern medicine study shows：The anticancer factor that its cartilage and ridge cartilage contain（Biologically effective activity
Composition-chondroitin sulfate）With certain anticancer reinforced immunological effect.Edible paddlefish fish, have promote longevity, enriching yin is strengthened
Sun, physical fitness, beneficial brain improving eyesight and the effect such as clearing heat and detoxicating, can also treat pain in the back, stomach trouble and alopecia etc..
Prior art such as Authorization Notice No. is the B of CN 101869172 Chinese invention patent, discloses a kind of active eel peptide
Production method, its production method is：The fresh eel or fresh jelly eel that selection has been slaughtered enter back into after meat grinder is rubbed, sterilized
First fermentation plant carries out degraded hydrolysis, and obtained hydrolysis clear liquid is passed through into enzyme deactivation by fish bony spur separation, and grease separates, ultrafiltration,
Active eel peptide is produced in UF membrane, vacuum concentration, raw fragrant processing, spray drying.Using biological enzymolysis and enzyme modification gentle
Under the conditions of carry out degraded hydrolysis, extraction concentration high activity eel polypeptide-eel peptide, but the enzyme solution can cause product to have raw meat
Bitter taste.
The content of the invention
It is an object of the invention to provide it is a kind of safely and effectively, toxic side effect is small, promotes hair follicle growth, make one to grow new
Hair, the compound based on duckbilled fish active peptides for improving white hair.
The present invention is directed to the problem of being mentioned in above-mentioned technology, and the technical scheme taken is：Based on duckbilled fish active peptides
Compound, composition and its parts by weight are：Duckbilled fish active peptides 2-4 parts, vitamin B1 0.2-0.4 parts, vitamin B2 0.1-
0.3 part, black sesame powder 18-24 parts, black bean powder 22-28 parts, soyabean protein powder 8-12 parts, skimmed milk power 5-9 parts, sea mustard powder 2-
3 parts, codonopsis pilosula powder 4-8 parts, ginger powder 0.5-0.8 parts, white granulated sugar 5-10 parts.The compound is safe and effective, and toxic side effect is small, uses
Raw material be all food common in our daily lifes, it is cheap, by diet both can reach regulation condition,
Promote hair follicle growth, grow new hair, improve the purpose of white hair.
The preparation method of compound based on duckbilled fish active peptides, including pretreatment, fluid nutrient medium are prepared, seed liquor
Preparation, fermentation medium are prepared, fermented, purifying, compound is prepared, and are concretely comprised the following steps：
The company of strain used and its purchase is：Melanomyces and brewer's yeast are purchased from Shanghai Fu Xiang bio tech ltd.
Pretreatment：By fillet boiling 40-50min, with water by weight being 1 after cooling:1.5-2.6 ratio homogenate, obtain
The flesh of fish is starched, standby；
Fluid nutrient medium is prepared：Tryptone 0.8-1.2 parts, yeast extract 0.4-0.6 parts, sodium chloride 0.8- are taken by weight
1.2 parts, distilled water 96-100 parts, it is well mixed, regulation pH is 7-8, standby；
It is prepared by seed liquor：The bacillus licheniformis activated is inoculated into aforesaid liquid culture medium, 11- is cultivated at 52-57 DEG C
13h, bacillus licheniformis seed liquor is produced, using high temperature resistant the lichen bacillus ferments, can suppress to cause under the high temperature conditions
The growth of germ, spoilage organisms and other miscellaneous bacterias, therefore culture medium, without sterilizing, this is also that existing use normal temperature strain is sent out
Ferment method prepares what active peptide can not be accomplished；
It is prepared by fermentation medium：The composition and its parts by weight of fermentation medium be：Flesh of fish slurry 92-96 parts, glucose 2-3 parts, chlorine
Change calcium 0.1-0.3 parts, frerrous chloride 0.2-0.4 parts, dipotassium hydrogen phosphate 0.2-0.5 parts, each dispensing taken by formula, be well mixed,
PH to 7-8 is adjusted, sealing, standby, the fermentation medium carbon, nitrogen enrich, and provide the energy for the growth and breeding of thalline, can make seed
It can be mushroomed out after inoculation, reach certain mycelial concentration, the thalline for enabling to have grown, which synthesizes rapidly, needs product, and non-sterilized step
Suddenly, energy consumption can be not only reduced, the activity of duckbilled flesh of fish endogenous proteinase can also be retained；
Fermentation：Seed liquor is inoculated into fermentation culture according to 8-11% inoculum concentration, 40-50h, nothing are cultivated at 52-57 DEG C
Bacterium air intake is 1-2.5vvm, is sterilized, centrifugation, the temperature of microbial fermentation and the peak enzymolysis-ability temperature of endogenous enzymes in the step
Degree is consistent so that endogenous enzymes are digested together with microbial fermentation energy perfect adaptation, hydrolysis is carried out simultaneously with fermentation, are carried
High preparation efficiency, reduces cost；
Purifying：Centrifugate is removed into macro-molecular protein, Ran Houtong by the ultrafiltration for the milipore filter that molecular cut off is 3-5KDa
Cross molecular cut off and obtain concentrate for the nanofiltration removal amino acid of 200-300Da NF membrane, freeze-drying obtains duck
Mouth fish active peptides, the amino acid sequence of the active peptides are
HSHACASYSRYYSVCGYHRGRCYCRCLRCRVLHPGKLCVCVNCSR, the active peptide can not only be thin by activating papilla
Born of the same parents' signal path regulates and controls hair follicle cycle so that the hair follicle being in hibernation enters growth period, induces the regeneration of hair follicle, and has
Increase vasopermeability, stimulate the function of angiogenesis, promote hair follicle growth, so as to reach trichogenous purpose；
Compound is prepared：Duckbilled fish active peptides, vitamin B1, vitamin B2, black sesame powder, black bean powder, big are measured by formula
Legumin powder, skimmed milk power, sea mustard powder, codonopsis pilosula powder, ginger powder, white granulated sugar, it is well mixed, produces more based on duckbilled fish activity
The compound of peptide, the compound is safe and effective, and toxic side effect is small, and the raw material used are all foods common in our daily lifes
Thing, it is cheap, regulation condition can have both been reached by diet, has promoted hair follicle growth, new hair has been grown, improves white hair
Purpose.
Compared with prior art, the advantage of the invention is that：1）Compound of the present invention is safe and effective, and toxic side effect is small, makes
Raw material are all food common in our daily lifes, cheap, and regulation body shape can have both been reached by diet
State, promote hair follicle growth, grow new hair, improve the purpose of white hair；2）Preparation method of the present invention has been sufficiently reserved duckbilled fish
The activity of endogenous proteinase so that endogenous enzymes are digested together with microbial fermentation energy perfect adaptation, make hydrolysis can with fermentation
Carry out simultaneously, improve preparation efficiency, reduce energy consumption, it is cost-effective；3）Preparation method action condition is gentle, more remains system
The biological activity of standby active peptides and aquatic product protein contained functional components in itself, and simplify the work of active peptide preparation
Sequence and the production cost for reducing active peptide, there is good application prospect.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
Based on the compound of duckbilled fish active peptides, composition and its parts by weight are：3 parts of duckbilled fish active peptides, vitamin B1
0.2 part, 0.3 part of vitamin B2,21 parts of black sesame powder, 23 parts of black bean powder, 9 parts of soyabean protein powder, 7 parts of skimmed milk power, undaria pinnitafida
2.5 parts of powder, 6 parts of codonopsis pilosula powder, 0.7 part of ginger powder, 6 parts of white granulated sugar.
The preparation method of compound based on duckbilled fish active peptides, including pretreatment, fluid nutrient medium are prepared, seed liquor
Preparation, fermentation medium are prepared, fermented, purifying, compound is prepared, and are concretely comprised the following steps：
1）Pretreatment：By fillet boiling 45min, with water by weight being 1 after cooling:1.8 ratio homogenate, flesh of fish slurry is obtained, it is standby
With；
2）Fluid nutrient medium is prepared：1 part of tryptone, 0.45 part of yeast extract, 1 part of sodium chloride, distilled water are taken by weight
100 parts, it is well mixed, regulation pH is 7.5, standby；
3）It is prepared by seed liquor：The bacillus licheniformis activated is inoculated into aforesaid liquid culture medium, 12h is cultivated at 54 DEG C,
Produce bacillus licheniformis seed liquor, using high temperature resistant the lichen bacillus ferments, can suppress under the high temperature conditions pathogenic bacteria,
Spoilage organisms and the growth of other miscellaneous bacterias, therefore culture medium, without sterilizing, this is also existing using normal temperature strain progress fermentation method
Prepare what active peptide can not be accomplished；
4）It is prepared by fermentation medium：The composition and its parts by weight of fermentation medium be：The flesh of fish starches 94 parts, 2 parts of glucose, calcium chloride
0.2 part, 0.2 part of frerrous chloride, 0.3 part of dipotassium hydrogen phosphate, each dispensing is taken by formula, is well mixed, adjust pH to 7.5, sealed,
Standby, the fermentation medium carbon, nitrogen are enriched, and the energy is provided for the growth and breeding of thalline, and seed can be made to be mushroomed out after being inoculated with,
Reach certain mycelial concentration, the thalline for enabling to have grown, which synthesizes rapidly, needs product, and non-sterilized step, can not only reduce energy
Consumption, the activity of duckbilled flesh of fish endogenous proteinase can also be retained；
5）Fermentation：Seed liquor is inoculated into fermentation culture according to 9% inoculum concentration, 44h, filtrated air are cultivated at 55 DEG C
Intake is 2vvm, is sterilized, centrifugation, and the temperature of microbial fermentation is consistent with the peak enzymolysis-ability temperature of endogenous enzymes in the step, makes
Endogenous enzymes enzymolysis is obtained together with microbial fermentation energy perfect adaptation, hydrolysis is carried out simultaneously with fermentation, improves to prepare and imitates
Rate, reduce cost；
6）Purifying：Centrifugate is removed into macro-molecular protein, Ran Houtong by the ultrafiltration for the milipore filter that molecular cut off is 4KDa
Cross molecular cut off and obtain concentrate for the nanofiltration removal amino acid of 200Da NF membrane, freeze-drying obtains duckbilled fish
Active peptides, the amino acid sequence of the active peptides are
HSHACASYSRYYSVCGYHRGRCYCRCLRCRVLHPGKLCVCVNCSR, the active peptide can not only be thin by activating papilla
Born of the same parents' signal path regulates and controls hair follicle cycle so that the hair follicle being in hibernation enters growth period, induces the regeneration of hair follicle, and has
Increase vasopermeability, stimulate the function of angiogenesis, promote hair follicle growth, so as to reach trichogenous purpose；
7）Compound is prepared：By formula measure duckbilled fish active peptides, vitamin B1, vitamin B2, black sesame powder, black bean powder,
Soyabean protein powder, skimmed milk power, sea mustard powder, codonopsis pilosula powder, ginger powder, white granulated sugar, it is well mixed, produces based on duckbilled fish activity
The compound of polypeptide, the compound is safe and effective, and toxic side effect is small, and the raw material used are all common in our daily lifes
Food, it is cheap, regulation condition can have both been reached by diet, has promoted hair follicle growth, new hair has been grown, improves in vain
The purpose of hair.
Embodiment 2：
Based on the compound of duckbilled fish active peptides, composition and its parts by weight are：2 parts of duckbilled fish active peptides, vitamin B1
0.4 part, 0.1 part of vitamin B2,22 parts of black sesame powder, 25 parts of black bean powder, 10 parts of soyabean protein powder, 7 parts of skimmed milk power, opotism
2 parts of vegetable powder, 5 parts of codonopsis pilosula powder, 0.6 part of ginger powder, 8 parts of white granulated sugar.
The preparation method of compound based on duckbilled fish active peptides, is concretely comprised the following steps：
1）By fillet boiling 50min, with water by weight being 1 after cooling:2 ratio homogenate, obtains flesh of fish slurry；
2）1 part of tryptone is taken by weight, 0.5 part of yeast extract, 1 part of sodium chloride, 98 parts of distilled water, is well mixed, and is adjusted
It is 8 to save pH, produces fluid nutrient medium；
3）The bacillus licheniformis activated is inoculated into fluid nutrient medium, 13h is cultivated at 57 DEG C, produces bacillus licheniformis
Seed liquor, using high temperature resistant the lichen bacillus ferments, pathogenic bacteria, spoilage organisms and other miscellaneous bacterias can be suppressed under the high temperature conditions
Growth, therefore culture medium is without sterilizing, and this is also existing using normal temperature strain to carry out fermentation method and prepare active peptide doing
Arrive；
4）The flesh of fish is taken to starch 93 parts, 3 parts of glucose, 0.18 part of calcium chloride, 0.25 part of frerrous chloride, dipotassium hydrogen phosphate by weight
0.4 part, it is well mixed, adjusts pH to 7.6, sealing, produce fermentation medium, the fermentation medium carbon, nitrogen enriches, and are thalline
Growth and breeding provides the energy, and seed can be made to be mushroomed out after being inoculated with, reach certain mycelial concentration, enable the thalline grown fast
Speed synthesis needs product, and non-sterilized step, can not only reduce energy consumption, can also retain the work of duckbilled flesh of fish endogenous proteinase
Property；
5）Seed liquor is inoculated into fermentation culture according to 8% inoculum concentration, 46h, filtrated air intake are cultivated at 57 DEG C
For 2.5vvm, sterilize, centrifuge, the temperature of microbial fermentation is consistent with the peak enzymolysis-ability temperature of endogenous enzymes in the step so that interior
Source enzyme enzymolysis makes hydrolysis to be carried out simultaneously with fermentation together with microbial fermentation energy perfect adaptation, improves preparation efficiency, drop
Low cost；
6）Centrifugate is removed into macro-molecular protein by the ultrafiltration for the milipore filter that molecular cut off is 4KDa, then passes through retention
Molecular weight removes amino acid for the nanofiltration of 300Da NF membrane and obtains concentrate, and it is more that freeze-drying obtains duckbilled fish activity
Peptide, the amino acid sequences of the active peptides are HSHACASYSRYYSVCGYHRGRCYCRCLRCRVLHPGKLCVCVNCSR, the work
Property peptide can not only regulate and control hair follicle cycle by activating hair papilla cell signal path so that the hair follicle that is in hibernation enters growth
Phase, the regeneration of hair follicle is induced, and with vasopermeability is increased, stimulate the function of angiogenesis, promote hair follicle growth, so as to
Reach trichogenous purpose；
7）By formula measure duckbilled fish active peptides, vitamin B1, vitamin B2, black sesame powder, black bean powder, soyabean protein powder,
Skimmed milk power, sea mustard powder, codonopsis pilosula powder, ginger powder, white granulated sugar, it is well mixed, produces the compounding based on duckbilled fish active peptides
Thing, the compound is safe and effective, and toxic side effect is small, and the raw material used are all food common in our daily lifes, price
It is cheap, regulation condition can have both been reached by diet, has promoted hair follicle growth, new hair has been grown, improves the purpose of white hair.
Embodiment 3：
Based on the compound of duckbilled fish active peptides, its preparation method is：By fillet boiling 48min, after cooling with water by weight
Than for 1:2.3 ratio is homogenized to obtain flesh of fish slurry；1.2 parts of tryptone, 0.4 part of yeast extract, sodium chloride 1 are taken by weight
Part, 98 parts of distilled water, it is well mixed, regulation pH obtains fluid nutrient medium for 7.8；The bacillus licheniformis activated is inoculated into liquid
In body culture medium, cultivate 11h at 56 DEG C and obtain bacillus licheniformis seed liquor；Flesh of fish slurry 92-96 parts, glucose 2- are taken by weight
3 parts, calcium chloride 0.1-0.3, frerrous chloride 0.2-0.4 parts, dipotassium hydrogen phosphate 0.2-0.5 parts, each dispensing is taken by formula, mixing is
Even, regulation pH to 7.8 obtains fermentation culture；Seed liquor is inoculated into fermentation culture according to 10% inoculum concentration, at 56 DEG C
46h is cultivated, filtrated air intake is 1.5vvm, is sterilized, centrifugation；Milipore filter by centrifugate by molecular cut off for 5KDa
Ultrafiltration, duckbilled fish active peptides are then obtained by the NF membrane nanofiltration that molecular cut off is 300Da, freeze-drying；By weight
Part take 4 parts of duckbilled fish active peptides, 0.2 part of vitamin B1,0.2 part of vitamin B2,23 parts of black sesame powder, 25 parts of black bean powder,
11 parts of soyabean protein powder, 7 parts of skimmed milk power, 3 parts of sea mustard powder, 7 parts of codonopsis pilosula powder, 0.8 part of ginger powder, 8 parts of white granulated sugar, mixing are equal
It is even, produce the compound based on duckbilled fish active peptides.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Leqing Rui Yao food technologies Co., Ltd
<120>Compound based on duckbilled fish active peptides
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 45
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Ala Ser Tyr Ser Arg Tyr Tyr Ser Val Cys Gly
1 5 10 15
Tyr His Arg Gly Arg Cys Tyr Cys Arg Cys Leu Arg Cys Arg Val Leu
20 25 30
His Pro Gly Lys Leu Cys Val Cys Val Asn Cys Ser Arg
35 40 45
Claims (10)
1. the compound based on duckbilled fish active peptides, it is characterised in that：The composition and its parts by weight of described compound be：Duck
Mouth fish active peptides 2-4 parts, vitamin B1 0.2-0.4 parts, vitamin B2 0.1-0.3 parts, black sesame powder 18-24 parts, black soya bean
Powder 22-28 parts, soyabean protein powder 8-12 parts, skimmed milk power 5-9 parts, sea mustard powder 2-3 parts, codonopsis pilosula powder 4-8 parts, ginger powder
0.5-.8 parts, white granulated sugar 5-10 parts.
2. the compound according to claim 1 based on duckbilled fish active peptides, it is characterised in that：Described duckbilled fish lives
The amino acid sequence of property polypeptide is HSHACASYSRYYSVCGYHRGRCYCRCLRCRVLHPGKLCVCVNCSR.
3. the compound according to claim 1 based on duckbilled fish active peptides, it is characterised in that：Described compound
Preparation method comprises the following steps
1）Pretreatment：By fillet boiling, it is homogenized after cooling with water, obtains flesh of fish slurry, it is standby；
2）Fluid nutrient medium is prepared：Dispensing is taken by weight, is well mixed, and regulation pH is 7-8, sealing, standby；
3）It is prepared by seed liquor：The bacillus licheniformis activated is inoculated into fluid nutrient medium and cultivated, produces seed liquor；
4）It is prepared by fermentation medium：Each dispensing is taken by formula, is well mixed, adjusts pH to 7-8, sealing is standby；
5）Fermentation：Seed liquor is inoculated into fermentation culture and cultivated, is passed through filtrated air, is sterilized, centrifugation；
6）Purifying：By centrifugate ultrafiltration, nanofiltration, freeze-drying obtains duckbilled fish active peptides；
7）Compound is prepared：Each dispensing is taken by formula, is well mixed, produces the compound based on duckbilled fish active peptides.
4. the preparation method of the compound according to claim 3 based on duckbilled fish active peptides, it is characterised in that：It is described
Step 1 in boiling 40-50min, fillet and water are by weight being 1:1.5-2.6.
5. the preparation method of the compound according to claim 3 based on duckbilled fish active peptides, it is characterised in that：It is described
Step 2 in fluid nutrient medium composition and its parts by weight be：Tryptone 0.8-1.2 parts, yeast extract 0.4-0.6 parts,
Sodium chloride 0.8-1.2 parts, distilled water 96-100 parts.
6. the preparation method of the compound according to claim 3 based on duckbilled fish active peptides, it is characterised in that：It is described
Step 3 in cultivation temperature be 52-57 DEG C, time 11-13h.
7. the preparation method of the compound according to claim 3 based on duckbilled fish active peptides, it is characterised in that：It is described
Step 4 in fermentation medium composition and its parts by weight be：Flesh of fish slurry 92-96 parts, glucose 2-3 parts, calcium chloride 0.1-
0.3, frerrous chloride 0.2-0.4 parts, dipotassium hydrogen phosphate 0.2-0.5 parts.
8. the preparation method of the compound according to claim 3 based on duckbilled fish active peptides, it is characterised in that：It is described
Step 5 in the inoculum concentration of seed liquor be 8-11%, cultivation temperature is 52-57 DEG C, time 40-50h.
9. the preparation method of the compound according to claim 3 based on duckbilled fish active peptides, it is characterised in that：It is described
Step 5 in filtrated air intake be 1~2.5vvm.
10. the preparation method of the compound according to claim 3 based on duckbilled fish active peptides, it is characterised in that：Institute
The molecular cut off of milipore filter is 3-5KDa in the step 6 stated, and the molecular cut off of NF membrane is 200-300Da.
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