CN107460140B - Preparation method of legionella active substance in bacillus HZ16 - Google Patents
Preparation method of legionella active substance in bacillus HZ16 Download PDFInfo
- Publication number
- CN107460140B CN107460140B CN201610388229.6A CN201610388229A CN107460140B CN 107460140 B CN107460140 B CN 107460140B CN 201610388229 A CN201610388229 A CN 201610388229A CN 107460140 B CN107460140 B CN 107460140B
- Authority
- CN
- China
- Prior art keywords
- bacillus
- legionella
- culture medium
- inoculating
- active substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K2035/11—Medicinal preparations comprising living procariotic cells
- A61K2035/115—Probiotics
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a bacillus capable of producing an active substance for inhibiting legionella and a method for preparing the active substance for inhibiting legionella by using the bacillus. The invention proves that the metabolite of the strain inhibits the activity of legionella for the first time, and the activity is taken as an evaluation standard to research the fermentation condition of the strain with stronger activity. Aims to provide a new method for producing an active substance for inhibiting legionella by using bacillus and provide scientific basis for further searching and developing effective medicaments for treating pneumonia.
Description
Technical Field
The invention relates to the field of development and utilization of microbial active substances, in particular to the activity of a bacillus strain, a process method for preparing bioactive components by fermentation and application of products of the bacillus strain in the aspect of new drug development.
Background
One strain of bacillus HZ16 showed a unique high selective inhibitory activity against legionella. Unlike the typical bacillus which produces broad-spectrum antibiotics, the strain has too weak or undetected inhibitory activity against gram-positive and gram-negative bacteria other than legionella. The broad-spectrum antibiotics have the disadvantages that the broad-spectrum antibiotics can kill other beneficial or harmless microorganisms while acting on one microorganism, and can generate general drug resistance when forming drug resistance, thereby bringing difficulties for treating other diseases in the future.
Bacillus is the largest genus of the family bacillaceae of the order bacillales and is also one of the three major sources for the production of medical antibiotics. The external environment has great influence on the generation of secondary metabolites of microorganisms, and different fermentation culture conditions can generate compounds with different structures and different biological activities. In order to obtain more valuable actives, the strains and fermentation conditions must be explored and studied.
Disclosure of Invention
The invention proves that the legionella activity of the bacillus strain metabolite is inhibited for the first time, and the fermentation condition for generating stronger activity is researched. The research and development of the biological pharmacy are developed by utilizing the characteristics of renewable biomass resources, easy obtainment, low cost, no pollution and the like. In order to achieve the purpose, the invention adopts the following scheme:
the invention relates to a bacillus capable of producing an anti-legionella active substance, which is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No. 12406. In another aspect, the invention also relates to the use of the bacillus species described above for the preparation of an active agent against legionella, preferably one that can be used for the preparation of a medicament for the treatment of pneumonia.
In another aspect, the invention relates to a method for preparing an active against legionella from bacillus HZ16, said method comprising the steps of:
inoculating the purified bacillus HZ16 to a culture medium, fermenting to obtain a solid culture colony, inoculating the solid culture colony to a shake flask culture medium, fermenting to obtain a fermentation liquid, freeze-drying a product obtained by fermentation to remove water, and extracting with an organic solvent to obtain a crude extract.
In a preferred embodiment of the invention, the pH of the medium is 4 to 8 or 4, 5, 6, 7, 8.
In bookIn a preferred embodiment of the invention, the culture medium is LB, complex medium, PDA, Chashi, M2、M250% SW, Gao's, etc.
The formula of the culture medium is as follows:
complex medium (g/L): calcium chloride 0.5, potassium dihydrogen phosphate (KH)2PO4)0.1, potassium chloride (KCl)0.05, magnesium sulfate (MgSO)4)0.1, glucose 20.0, peptone 15.
PDA medium (g/L): potato 200, glucose 200, 1.1% agar.
Chase medium (g/L) sucrose 30, NaNO3 3，K2HPO4 1，FeSO4 0.01，KCl 0.5，MgSO4·7H20 0.5。
M2Culture medium: malt extract 10, glucose 4, yeast extract 4.
M250% SW Medium: malt extract 10, glucose 4, yeast extract 4, ferric citrate 0.05, NaCl 9.725, MgCl2·6H2O 4.4，Na2SO4 1.72，CaCl20.9, sodium hydrogen phosphate 0.01, SiO20.0075。
Gao's medium (g/L): starch 20, KNO3 1，K2HPO4 0.5，MgSO4·7H20 0.5，NaCl0.5，FeSO4·7H20 0.01。
LB medium (g/L): tryptone 10, yeast extract 5, NaCl 10.
In a preferred embodiment of the invention, the fermentation temperature is 25, 28, 30, 32 ℃.
In a preferred embodiment of the present invention, the fermentation time is 1 to 5 days.
In a preferred embodiment of the invention, the shaking flask culture is kept still at a rotating speed of 100, 180 and 230 r/min.
In a preferred embodiment of the present invention, the organic solvent is dichloromethane, ethyl acetate, or n-butanol.
The invention proves that the metabolite of the strain inhibits the activity of legionella for the first time, and the activity is taken as an evaluation standard to research the fermentation condition of the strain with stronger activity. Aims to provide a new method for producing an active substance for inhibiting legionella by using bacillus and provide scientific basis for further searching and developing effective medicaments for treating pneumonia.
Detailed Description
For a further understanding of the present invention, preferred embodiments of the present invention will be described in detail below with reference to examples. It is to be understood that such description is merely illustrative of the features and advantages of the present invention, and is not intended to limit the scope of the claims.
Microbiological information
Bacillus HZ16 was isolated and purified from laboratory contaminated Legionella plates and showed unique high selective activity against Legionella, and showed inhibitory activity against all tested Legionella (L.pneumophila Phoil. JR32; L.pneumophila Corby; L.gormanii; L.dumofii; L.microdadei; L.israelensis; L.jordania; L.hackeliae; L.longbeaconiae) while gram-positive bacteria other than Legionella (Listeria monocytogenes; Bacillus megaterium; Streptomyces pneconibacteriae; Staphylococcus aureus; Pseudomonas aurea; Mycobacterium) and negative bacteria (Klebsiella aeaee; Escherichia coli HB 101; Pseudomonas aeruginosa. 1; Pseudomonas aeruginosa. subunit P. III; Pseudomonas aeruginosa-21. Bacillus pyogenes; Pseudomonas aeruginosa III; Pseudomonas aeruginosa-3; Pseudomonas aeruginosa-2. Bacillus pyogenes; Pseudomonas aeruginosa-P.
The Bacillus HZ16 is preserved in China general microbiological culture Collection center (CGMCC), the preservation address is No. 3 of West Lu No.1 of North Cheng in the morning area of Beijing, the preservation number is CGMCC No.12406, the preservation date is 2016, 4 and 28 days, and the Bacillus HZ16 is classified and named as Bacillus sp.
Example 1:
the preparation method of the legionella inhibiting active substance in the bacillus HZ16 comprises the following steps:
step one, inoculating the purified bacillus HZ16 to an LB solid culture medium with the pH value of 5 under the aseptic condition, and culturing for 1 day at the temperature of 28 ℃ to obtain a white solid colony;
step two, inoculating the solid strain into a 300 ml conical flask filled with 150 ml of LB culture medium with pH of 5;
placing the inoculated shake flask in a biological incubator, and culturing for 1 day at 28 ℃ under the condition of 180r/min to obtain fermentation liquor;
step four, removing water by utilizing a freeze drying technology, and comparing the dry weight of the obtained solid;
step five, extracting by using an organic solvent ethyl acetate to obtain a crude extract, and concentrating for later use;
and step six, verifying that the bioactive peptide obtained in the example 1 has high selectivity and inhibits the biological activity of legionella by using a Kirby-Bauer (K-B) paper diffusion method.
Dissolving the crude extract to be detected obtained in the fifth step in DMSO to prepare a solution of 50 mu g/ml. 20 μ l of the solution of the sample to be tested was added dropwise to the sterilized paper sheets under sterile conditions. DMSO was used as a blank control and pure erythromycin as a positive control. The DMSO blank has no inhibition effect, and the erythromycin inhibition zone is 20 mm. Adding 1mL of bacteria solution into BCYE agar culture medium (yeast powder 10.0g/L, ACES buffer solution 10.0g/L, activated carbon 2.0g/L, alpha-ketoglutaric acid 1.0g/L, agar 15.0g/L, pH 6.85), coating with sterile coating rod, attaching paper sheet with sample solution, and heating at 37 deg.C with 5% CO2The culture box is cultured for 48h, and the inhibition circle of 20mm is observed.
Example 2:
step one, inoculating the purified bacillus HZ16 to an LB culture medium with the pH value of 8 under the aseptic condition, and culturing for 1 day at the temperature of 28 ℃ to obtain a white solid colony;
step two, inoculating the solid hyphae into M containing 150 ml of pH 8250% SW medium in 300 ml Erlenmeyer flasks;
placing the inoculated shake flask in a biological incubator, and culturing for 4 days at 28 ℃ at 200r/min to obtain fermentation liquor;
step four, removing water by utilizing a freeze drying technology, and comparing the dry weight of the obtained solid;
step five, extracting by using an organic solvent ethyl acetate to obtain a crude extract, and concentrating for later use;
step six, dissolving the crude extract by DMSO to prepare a solution of 50 mu g/mL, and performing the legionella inhibition activity test same as the step six in the example 1, wherein the inhibition cycle size of the solution is 22mm in 48 hours.
Example 3:
step one, inoculating the purified bacillus HZ16 to a Gauss culture medium with the pH value of 8 under the aseptic condition, and culturing for 4 days at the temperature of 28 ℃ to obtain a white solid colony;
step two, inoculating the solid hyphae into a 300 ml conical flask filled with 150 ml of a Gao's medium with the pH value of 8;
placing the inoculated shake flask in a biological incubator, and culturing for 4 days at 28 ℃ at 200r/min to obtain fermentation liquor;
step four, removing water by utilizing a freeze drying technology, and comparing the dry weight of the obtained solid;
step five, extracting by using an organic solvent ethyl acetate to obtain a crude extract, and concentrating for later use;
step six, dissolving the crude extract by DMSO to prepare a solution of 50 mu g/mL, and performing the legionella inhibition activity test same as the step six in the example 1, wherein the inhibition cycle size is 18mm in 48 hours.
Example 4:
step one, inoculating the purified bacillus HZ16 to a Gauss culture medium with the pH value of 8 under the aseptic condition, and culturing for 4 days at the temperature of 28 ℃ to obtain a white solid colony;
step two, inoculating the solid hyphae into a 300 ml conical flask filled with 150 ml of a Gao's medium with the pH value of 8;
placing the inoculated shake flask in a biological incubator, and culturing for 4 days at 28 ℃ at 200r/min to obtain fermentation liquor;
step four, removing water by utilizing a freeze drying technology, and comparing the dry weight of the obtained solid;
step five, extracting by using an organic solvent ethyl acetate to obtain a crude extract, and concentrating for later use;
step six, dissolving the crude extract by DMSO to prepare a solution of 50 mu g/mL, and performing the legionella inhibition activity test same as the step six in the example 1, wherein the inhibition ring size is 25mm in 48 hours.
Example 5:
step one, inoculating the purified bacillus HZ16 to a Chashi culture medium with the pH value of 8 under the aseptic condition, and culturing for 4 days at the temperature of 28 ℃ to obtain a white solid colony;
inoculating the solid hyphae into a 300 ml conical flask filled with 150 ml of Chashi culture medium with the pH value of 8;
placing the inoculated shake flask in a biological incubator, and culturing for 4 days at 28 ℃ at 200r/min to obtain fermentation liquor;
step four, removing water by utilizing a freeze drying technology, and comparing the dry weight of the obtained solid;
step five, extracting by using an organic solvent ethyl acetate to obtain a crude extract, and concentrating for later use;
step six, dissolving the crude extract by DMSO to prepare a solution of 50 mu g/mL, and performing the legionella inhibition activity test same as the step six in the example 1, wherein the inhibition cycle size of the solution is 17mm in 48 hours.
Example 6:
step one, inoculating the purified bacillus HZ16 to an LB culture medium with the pH value of 8 under the aseptic condition, and culturing for 4 days at the temperature of 28 ℃ to obtain a white solid colony;
step two, inoculating the solid hyphae into a 300 ml conical flask filled with 150 ml of LB culture medium with the pH value of 8;
placing the inoculated shake flask in a biological incubator, and culturing for 4 days at 28 ℃ at 200r/min to obtain fermentation liquor;
step four, removing water by utilizing a freeze drying technology, and comparing the dry weight of the obtained solid;
step five, extracting by using an organic solvent ethyl acetate to obtain a crude extract, and concentrating for later use;
step six, dissolving the crude extract by DMSO to prepare a solution of 50 mu g/mL, and performing the legionella inhibition activity test same as the step six in the example 1, wherein the inhibition cycle size is 24mm in 48 hours.
Example 7:
step one, inoculating the purified bacillus HZ16 to M with pH of 8 under aseptic condition2Culturing on a culture medium at 28 ℃ for 4 days to obtain white solid colonies;
step two, inoculating the solid hyphae into a culture medium containing 7 liters of M with the pH value of 82In a fermenter of the culture medium;
step three, culturing the medium for 6 days at 28 ℃ and under the condition of 200r/min, and fermenting to obtain fermentation liquor;
step four, sampling once every 24 hours, removing water by utilizing a freeze drying technology, and comparing the dry weight of the obtained solid;
step five, extracting by using an organic solvent ethyl acetate to obtain a crude extract, and concentrating for later use;
step six, dissolving the crude extract by DMSO to prepare a solution of 50 mu g/mL, and carrying out the legionella activity inhibition test same as the step six in the example 1. The size of the inhibition zone of the fermentation product is 20mm in 24 hours; the size of a product inhibition zone is 23mm in 48 hours; the size of a product inhibition zone is 20mm in 72 hours; the size of the inhibition ring of the product is 21mm in 96 hours; the size of the inhibition zone of the product is 18mm after 117 hours; the product inhibition zone size was 15mm at 137 hours.
Example 8:
step one, inoculating the purified bacillus HZ16 to an LB culture medium with the pH value of 6 under the aseptic condition, and culturing for 2 days at the temperature of 28 ℃ to obtain a white solid colony;
inoculating the solid hyphae into a fermentation tank filled with 7 liters of LB culture medium with the pH value of 6;
step three, culturing the mixture for 5 days at the temperature of 28 ℃ and under the condition of 200r/min, and fermenting to obtain fermentation liquor;
step four, sampling once every 24 hours, removing water by utilizing a freeze drying technology, and comparing the dry weight of the obtained solid;
step five, extracting by using an organic solvent ethyl acetate to obtain a crude extract, and concentrating for later use;
step six, dissolving the crude extract by DMSO to prepare a solution of 50 mu g/mL, and carrying out the legionella activity inhibition test same as the step six in the example 1. The size of the inhibition zone of the fermentation product is 16mm in 24 hours; the size of a product inhibition ring is 17mm in 48 hours; the size of a product inhibition zone is 16mm in 72 hours; the size of the inhibition ring of the product is 21mm in 96 hours; the product inhibition zone size was 19mm at 117 hours; the 137 hour product inhibition zone size is 13 mm.
Example 9:
step one, inoculating the purified bacillus HZ16 to M with pH of 5 under aseptic condition2Culturing on a culture medium at 28 ℃ for 1 day to obtain white solid colonies;
step two, inoculating the solid hyphae into a culture medium containing 7 liters of M with the pH value of 82In a fermenter of the culture medium;
step three, culturing for 1 day at 28 ℃, 100r/min, 200r/min and 300r/min, and fermenting to obtain fermentation liquor;
step four, removing water by utilizing a freeze drying technology, and comparing the dry weight of the obtained solid;
step five, extracting by using an organic solvent ethyl acetate to obtain a crude extract, and concentrating for later use;
step six, dissolving the crude extract by DMSO to prepare a solution of 50 mu g/mL, and performing the same legionella inhibition activity test as the step six in the example 1, wherein the inhibition ring size of the fermentation product is 18mm at 24 hours and 100 r/min; the size of the inhibition ring is 24mm at 200 r/min; the size of the inhibition ring is 20mm at 300 r/min.
Example 10:
step one, inoculating the purified bacillus HZ16 to M with pH of 5 under aseptic condition2Culturing on a culture medium at 28 ℃ for 1 day to obtain white solid colonies;
step two, inoculating the solid hyphae into a culture medium containing 7 liters of M with the pH value of 82In a fermenter of the culture medium;
step three, culturing for 5 days at 28 ℃, 100r/min, 200r/min and 300r/min to obtain fermentation liquor;
step four, removing water by utilizing a freeze drying technology, and comparing the dry weight of the obtained solid;
step five, extracting by using an organic solvent ethyl acetate to obtain a crude extract, and concentrating for later use;
step six, dissolving the crude extract by DMSO to prepare a solution of 50 mu g/mL, and performing the same legionella inhibition activity test as the step six in the example 1, wherein the inhibition ring size of the fermentation product is 20mm at 16 hours and 100 r/min; the size of the inhibition ring is 23mm at 200 r/min; the size of the inhibition ring is 21mm at 300 r/min.
The above description of the embodiments is only intended to facilitate the understanding of the method of the invention and its core idea. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
Claims (7)
1. Bacillus HZ16 (for producing anti-legionella active substance)Bacillussp.) in the preparation of the active substance against legionella, wherein the bacillus HZ16 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC number 12406.
2. The method of producing an active against legionella bacteria of the bacillus HZ16 of claim 1, said method of production comprising the steps of:
inoculating the purified bacillus HZ16 to a culture medium, fermenting to obtain a solid culture colony, inoculating the solid culture colony to a shake flask culture medium, fermenting to obtain a fermentation liquid, freeze-drying a product obtained by fermentation to remove water, and extracting with an organic solvent to obtain a crude extract;
the pH value of the culture medium is 4-8.
3. According to claim2, the culture medium is LB, PDA, Chashi, M2、M250% seawater, Gao's.
4. The method of claim 2, wherein the fermentation temperature is 25-32 ℃.
5. The method according to claim 2, wherein the fermentation time is 1 to 5 days.
6. The method as claimed in claim 2, wherein the shaking flask culture rotation speed is 100-230 r/min.
7. The method of claim 2, wherein the organic solvent is one or more selected from dichloromethane, ethyl acetate and n-butanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610388229.6A CN107460140B (en) | 2016-06-02 | 2016-06-02 | Preparation method of legionella active substance in bacillus HZ16 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610388229.6A CN107460140B (en) | 2016-06-02 | 2016-06-02 | Preparation method of legionella active substance in bacillus HZ16 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107460140A CN107460140A (en) | 2017-12-12 |
| CN107460140B true CN107460140B (en) | 2020-11-13 |
Family
ID=60545653
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610388229.6A Active CN107460140B (en) | 2016-06-02 | 2016-06-02 | Preparation method of legionella active substance in bacillus HZ16 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107460140B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102655745A (en) * | 2009-11-12 | 2012-09-05 | 西格纳姆生物科学公司 | Use of anti-bacterial agents for the treatment of epithelial-related conditions |
| CN103108638A (en) * | 2010-04-15 | 2013-05-15 | 海洋聚合物技术公司 | Anti-bacterial applications of poly -n-acetylglucosamine nanofibers |
| CN104955466A (en) * | 2012-11-23 | 2015-09-30 | 赛里斯治疗公司 | Synergistic bacterial compositions and methods of production and use thereof |
-
2016
- 2016-06-02 CN CN201610388229.6A patent/CN107460140B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102655745A (en) * | 2009-11-12 | 2012-09-05 | 西格纳姆生物科学公司 | Use of anti-bacterial agents for the treatment of epithelial-related conditions |
| CN103108638A (en) * | 2010-04-15 | 2013-05-15 | 海洋聚合物技术公司 | Anti-bacterial applications of poly -n-acetylglucosamine nanofibers |
| CN104955466A (en) * | 2012-11-23 | 2015-09-30 | 赛里斯治疗公司 | Synergistic bacterial compositions and methods of production and use thereof |
Non-Patent Citations (2)
| Title |
|---|
| Antimicrobial Activities of Bacillus Strains containing biosynthetic genes against Legionella pneumophila;Nihal et al;《The Battle Against Microbial Pathogens: Basic Science，Technological Advances and Educational Programs (A.Méndez-Vilas,Ed.)》;20151231;摘要及表2 * |
| ISOLATION AND CHARACTERIZATION OF Bacillus thuringiensis ISOLATED FROM HAZELNUT FIELDS IN TURKEY;Nalcacioglu et al;《Fresenius Environmental Bulletin》;20021231;全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107460140A (en) | 2017-12-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106957805B (en) | Bacillus GBacilus-9 strain with bacteriostatic effect and separation method and application thereof | |
| CN102282248A (en) | Methods for isolating bacteria | |
| CN110982734B (en) | Marine-derived bacillus subtilis 2713, antibacterial substance and preparation method and application thereof | |
| WO2022111287A1 (en) | Strain for degrading deoxynivalenol and use thereof | |
| CN106434783A (en) | Benzopyrone compound, benzopyrone compound preparation method and application of benzopyrone compound to preparation of antibacterial medicines | |
| WO2018133399A1 (en) | Method for producing p-hydroxybenzaldehyde using microorganism | |
| JP2018524000A (en) | Use of microbacterial strains for the production of antibacterial agents | |
| CN105130963A (en) | Separation and extraction and structure identification of novel quorum sensing inhibitor and application thereof | |
| Frikha Dammak et al. | Antagonistic properties of some halophilic thermoactinomycetes isolated from superficial sediment of a solar saltern and production of cyclic antimicrobial peptides by the novel isolate Paludifilum halophilum | |
| CN104928220B (en) | The water Rhein sea of one plant of ocean sludge source is write from memory Salmonella bacterial strain and its application | |
| CN106754497A (en) | A kind of Ke Leishi pneumobacilluses and its microbial inoculum and preparation method and application | |
| CN113862199A (en) | Degrading strain of benzonitrile herbicide and microbial inoculum produced by degrading strain | |
| CN111778178B (en) | Application of marine streptomyces griseoflavus HN60 in antibacterial aspect | |
| CN103232957B (en) | High-efficiency antibacterial rhamnolipid high-yield strain and application thereof | |
| CN107460140B (en) | Preparation method of legionella active substance in bacillus HZ16 | |
| CN101921250A (en) | Bisabolane sesquiterpene derivative and preparation method and application thereof | |
| CN100424168C (en) | Active extract of a strictly anaerobic marine bacterium, and its preparing method and use | |
| CN109897869B (en) | Biological activated carbon cultured by high-sugar wastewater and using ficus microcarpa leaves as carbon source carrier | |
| CN102344895B (en) | Method for screening sodium chloride-resistant bacillus licheniformis | |
| CN107574130B (en) | Application of coral coccus in predation of drug-resistant bacteria and preparation of drug for inhibiting drug-resistant bacteria | |
| CN110317764B (en) | Compound microbial agent and application thereof | |
| CN103849589B (en) | One strain quinclorac degradation bacteria and uses thereof and using method | |
| CN107460139B (en) | Preparation method of cytotoxic active compound in bacillus HZ16 | |
| CN101880302B (en) | Polyhydroxylated sterol derivative as well as preparation and application thereof | |
| Falzone et al. | Development of a chemically defined medium for the production of the antibiotic platensimycin by S treptomyces platensis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |