CN109897869B - Biological activated carbon cultured by high-sugar wastewater and using ficus microcarpa leaves as carbon source carrier - Google Patents
Biological activated carbon cultured by high-sugar wastewater and using ficus microcarpa leaves as carbon source carrier Download PDFInfo
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Abstract
The invention belongs to the technical field of biological activated carbon, and particularly relates to biological activated carbon which is cultured by high-sugar wastewater and takes banyan leaves as a carbon source carrier. Placing a porous carbon carrier rich in surface defects and using ficus microcarpa leaves as a carbon source in a culture medium, inoculating the mixed strain into high-sugar wastewater in two steps, and performing shaking co-culture to obtain the biological activated carbon. The two-step culture is favorable for the microbial strains added in the early stage to hydrolyze oligosaccharide or cellulose in the high-sugar wastewater into micromolecular saccharides, and the generated intermediate metabolite can be used as a carbon source for the growth and the propagation of the microbial strains added in the later stage. Organic matters and pollutants adsorbed on carrier carbon are respectively and efficiently utilized and synergistically degraded by microorganisms, so that the water purifying agent has double purifying effects of carbon physical adsorption and biological enzyme degradation, is hydrophilic but easy to precipitate, and is very suitable for large-scale treatment of high-sugar wastewater.
Description
Technical Field
The invention belongs to the technical field of biological activated carbon, and particularly relates to biological activated carbon which is cultured by high-sugar wastewater and takes banyan leaves as a carbon source carrier.
Technical Field
At present, dairy products such as yoghourt, fresh milk, pure milk, milk powder and the like enter every family at home and abroad to become indispensable nutriments for a plurality of people, but a certain amount of waste water is discharged in the processing process of the dairy products, and the dairy products mainly come from bottle washing water, brushing and filling water, flushing water and the like. The wastewater mainly contains a large amount of soluble organic matters (saccharides, fatty acids, proteins, starch and the like), and the organic matters are very easily degradable substances although the content of the organic matters is low, and can be degraded very quickly after being discharged into a water body, so that the environmental pollution is caused.
In the test and application process, the activated carbon is usually used as an adsorbent for sewage pollutants, can improve the chromaticity and turbidity of sewage, and simultaneously has a macroporous, mesoporous and microporous structure, so that the activated carbon can also perform adsorption on small molecules and heavy metal ions. And the activated carbon can be used as a carrier of the microbial water purifying agent for sewage purification treatment, and the bacterial carbon water purifying agent formed by fixing the composite bacterial strain on the carbon carrier has ideal sewage purification effect.
Publication No. CN 107473404A, publication No. CN 107434305A and publication No. CN 107512780A all disclose a bacterial carbon water purifying agent, a carbon carrier containing a hydrophilic group is placed in a culture medium, and simultaneously, a composite strain is inoculated to the culture medium, and the bacterial carbon water purifying agent is prepared by shaking co-culture. However, when the culture medium is shake co-cultured, the culture medium is beef extract peptone medium or Luria-Bertani medium. Compared with the culture method adopting high-sugar wastewater, the bacterial carbon water purifying agent obtained by culturing the beef extract peptone culture medium or the Luria-Bertani culture medium needs an adaptation period of 2-3d in practical application, and the service life is about 24d-28 d.
The ficus microcarpa leaf is a leaf of ficus microcarpa of the family Moraceae, the main components of the ficus microcarpa leaf are brass compounds (containing oxygen-containing substituent groups such as hydroxyl, methoxy, oxyl, isopentenyloxyl and the like), tannin (polyphenol compounds containing rich hydroxyl oxygen-containing functional groups) and triterpenoid saponin (polyhydroxy glucose, galactose and rhamnose generated through hydrolysis), the two plant leaves with rich oxygen-containing groups are activated and etched in anhydrous molten solid alkali at the temperature of 166 plus 200 ℃ to obtain surface defects and pore structures, and partial plant oxygen-containing groups remained after water extraction and calcination treatment can enhance the hydrophilicity of the carbon surface and the adsorption effect of the carbon surface with organic matter groups of high-sugar wastewater.
Disclosure of Invention
The invention aims to provide biological activated carbon which is cultured by high-sugar wastewater and takes ficus microcarpa leaves as a carbon source carrier, aiming at the defects of the prior art. The water purifying agent prepared by the invention has the double-circulation purification effect of carbon physical adsorption and enzyme catalytic degradation, realizes the resource utilization of high-sugar wastewater by microorganisms, simultaneously takes natural agricultural and forestry wastes as a carbon source, and applies the synthesized material to pollutant treatment, thereby realizing the resource utilization of the agricultural and forestry wastes and the double pollution treatment of the wastes and sewage.
In order to achieve the purpose, the invention adopts the following technical scheme:
preparation of biological activated carbon cultured by high-sugar wastewater and using ficus microcarpa leaves as carbon source carrierThe method comprises the following steps: placing the porous carbon carrier rich in surface defects into high-sugar wastewater to prepare a culture solution; then Candida utilis (A), (B) andCandida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Saccharopolyspora fuliginosa (A), (B), (C)Saccharopolyspora hirsuta) And Acinetobacter arborescens (Zoogloea ramigera) Inoculating the strain into culture solution, shake co-culturing, and inoculating Bacillus coagulans (Bacillus coagulans)Paenibacillus curdlanolyticus) Bacillus brevis (B), (B)Brevibacillus parabrevis) And Phytoplankton: (A. plankton: (B.))Sphaerotilus natans) Inoculating the strain into a culture solution, and performing shaking co-culture and drying to obtain biological activated carbon; the porous carbon carrier rich in surface defects is prepared by taking ficus microcarpa leaves as a raw material, placing the ficus microcarpa leaves in NaOH and KOH molten alkali liquor, and carrying out melting, ethanol washing and nitrogen atmosphere calcination.
The preparation method of the porous carbon carrier rich in surface defects comprises the following steps:
(1) placing 90g of mixed alkali of NaOH and KOH in an inner container of a reaction kettle of 200 ml, and melting for 2h at the temperature of 165-200 ℃ to obtain clear molten alkali liquor;
(2) adding 100g of ficus microcarpa leaves as a raw material into clear molten alkali liquor to react for 4-12 hours to generate a carbon precursor material;
(3) washing the carbon precursor material with water and absolute ethyl alcohol in sequence, and drying at 60 ℃ for 12h to obtain a powdered carbon precursor;
(4) and calcining the powder carbon precursor in a nitrogen atmosphere at 500 ℃ for 2h to obtain the porous surface defect-rich carbon powder carrier material.
In the mixed alkali in the step (1), the mass ratio of NaOH to KOH is 46.5: 43.5.
The preparation method of the biological activated carbon cultured by the high-sugar wastewater and taking ficus microcarpa leaves as a carbon source carrier comprises the following specific steps:
1) adding 10g of porous carbon carrier rich in surface defects into 50-150 ml of high-sugar wastewater to prepare a culture solution; candida utilis (A), (B) and (C)Candida utilis) Bacillus subtilis (B.subtilis) (B.subtilis)Bacillus subtilis) Saccharopolyspora fuliginosa (A), (B), (C)Saccharopolyspora hirsuta) Andacinetobacter arborescens (A), (B), (C)Zoogloea ramigera) The activated bacteria liquid is mixed according to the mass ratio of 4:3:2:1, then the mixed bacteria liquid is inoculated into the culture solution according to the inoculation amount of 3wt% -5 wt%, and the culture is carried out for 12h under the conditions of 30 ℃ and 150 rpm;
2) then the bacillus coagulans (B) is addedPaenibacillus curdlanolyticus) Bacillus brevis (B), (B)Brevibacillus parabrevis) And Phytoplankton: (Sphaerotilus natans) The activated bacteria liquid is mixed according to the mass ratio of 3:3:4, then the mixed bacteria liquid is inoculated into the culture liquid according to the inoculation amount of 1wt% -2 wt%, the culture is carried out for 20-22h under the conditions of 30 ℃ and 150 rpm, finally the carbon carrier is centrifugally separated out and is placed in a constant temperature box at 20-30 ℃ for drying, and the water purifying agent is prepared.
A biological activated carbon prepared by the preparation method as described above: the effective viable count on the porous carbon carrier rich in surface defects is 5.4 multiplied by 108～7.8×108CFU/g。
Application of the biological activated carbon in treatment of high-sugar wastewater.
The biological activated carbon which is cultured by the high-sugar wastewater and takes the ficus microcarpa leaves as the carbon source carrier is stored at 4 ℃, and is placed at normal temperature for 8-12 hours before use to recover the biological activity, and then is applied to the aspect of treating the high-sugar wastewater.
The species used in the above raw materials may be those which are conveniently isolated from nature by conventional species identification and isolation methods by those skilled in the art, or those which are publicly available through commercial channels. The strain adopted by the invention can be purchased from bacterial seed libraries such as the China general microbiological collection center, the Guangdong province microbiological collection center and the like.
The strains are cultured according to a slant strain preservation culture medium:
i, a candida utilis culture medium: 1.0g of glucose, 1.8g of KCl, 2.5g of yeast extract, 8.2g of sodium acetate, 15-20g of agar and 1000mL of distilled water, and the pH value is natural; packaging into test tubes after dissolving, and sterilizing for 20min at 115 deg.C by wet heat;
II, culture media of bacillus subtilis, leptospirillum, bacillus coagulans, bacillus brevis and olivil: 10.0g of tryptone, 5.0g of yeast extract, 10.0g of NaCl, 15-20g of agar and 1000mL of distilled water, wherein the pH value is 7.2;
III culture medium of Saccharopolyspora fuliginosa and Volvocamphrena sp: sucrose 30.0g, NaNO3 2.0g、K2HPO4·3H2O 1.0g、KCl 0.5g、MgSO4·7H2O 0.5g、FeSO4·7H20.01g of O, 15-20g of agar and 1000mL of distilled water, and the pH value is 7.0-7.2.
The invention has the beneficial effects that:
1) the invention takes the agricultural and forestry waste-ficus microcarpa leaves as the raw material, reacts in low-temperature molten alkali and is calcined to prepare the porous defect-rich carbon carrier, thereby avoiding the defect that toxic polluting gas is generated when the carbon is etched by using concentrated acid in the conventional method, and the charged groups on the surface of the carbon carrier and H in water+/OH-The electrostatic interaction is generated, so that the carbon carrier has better dispersibility in water, the problem of hydrophobicity of a common carbon carrier is solved, the contact probability with pollutants is increased, and meanwhile, the charged groups on the surface of the carrier carbon can generate electrostatic interaction with lone-pair electrons and hydrogen protons of pollutants, so that the adsorption efficiency of the pollutants is improved; the fused solid alkali environment enables the carbon material to obtain surface defects and secondary pore structures, provides more active sites for adsorption reaction, and enhances the adsorption effect on pollutants. The material synthesized by taking the agricultural and forestry wastes as the carbon source is applied to pollutant treatment, so that the resource utilization of the agricultural and forestry wastes and the double pollution treatment of the wastes and sewage are realized;
2) the biological activated carbon cultured by the high-sugar wastewater can take pollutants in the high-sugar wastewater as a nutrient substrate for self growth and propagation, so that the resource utilization of microorganisms on the high-sugar wastewater is realized; the products of microbial metabolism can be used as nutrient substrates for mutual utilization, and the biological activity of the microbes is improved to promote the synergistic degradation effect of the microbes on pollutants;
3) the high-sugar wastewater is used as the culture solution, so that the microbial activity in the water purifying agent is higher, the adaptability in the high-sugar wastewater is stronger, and the service life is longer in actual use;
4) the biological activated carbon has double purification effects of carbon physical adsorption and microbial enzyme degradation, has stable decontamination effect, is easy to treat, and is suitable for large-scale treatment of high-sugar wastewater.
Detailed Description
For further disclosure, but not limitation, the present invention is described in further detail below with reference to examples.
A biological activated carbon which is cultured by high-sugar wastewater and takes banyan leaves as a carbon source carrier comprises the following specific contents:
1. preparation of porous defect-rich carbon carrier with ficus microcarpa leaves as carbon source
1) Placing 90g of mixed alkali of NaOH and KOH in an inner container of a reaction kettle of 200 ml, and placing the inner container in a reactor at 165-200 ℃ for melting for 2h to obtain clear molten alkali liquor;
2) adding 100g of ficus microcarpa leaves as a raw material into clear molten alkali liquor to react for 4-12 hours to generate a carbon precursor material;
3) washing the carbon precursor material with water and absolute ethyl alcohol in sequence, and drying at 60 ℃ for 12h to obtain a powdered carbon precursor;
4) calcining the powdered carbon precursor in a nitrogen atmosphere at 500 ℃ for 2h to obtain a porous defect-rich carbon powder carrier material;
2. culturing high-sugar wastewater:
1) adding 10g of porous defect-rich carbon carrier into 50-150 ml of high-sugar wastewater to prepare a culture solution; candida utilis (A), (B) and (C)Candida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Saccharopolyspora fuliginosa (A), (B), (C)Saccharopolyspora hirsuta) And Acinetobacter genus Brandychium: (A)Zoogloea ramigera) The activated bacteria liquid is mixed according to the mass ratio of 4:3:2:1, then the mixed bacteria liquid is inoculated into the culture solution according to the inoculation amount of 3wt% -5 wt%, and the culture is carried out for 12h under the conditions of 30 ℃ and 150 rpm;
2) then the bacillus coagulans (B) is addedPaenibacillus curdlanolyticus) Bacillus brevis (B), (B)Brevibacillus parabrevis) And Phytoplankton: (A. plankton: (B.))Sphaerotilus natans) The activated bacteria liquid is mixed according to the mass ratio of 3:3:4, then the mixed bacteria liquid is inoculated into the culture solution according to the proportion of 1wt% -2 wt% of the inoculum size,culturing at 30 deg.C and 150 rpm for 20-22h, centrifuging to separate carbon carrier, and oven drying at 20-30 deg.C in a constant temperature oven to obtain water purifying agent.
The effective viable count on the carbon carrier is 5.4 multiplied by 108～7.8×108CFU/g; the biological active carbon preserved at the temperature of 4 ℃ is placed at normal temperature for 8-12 hours before use to recover the biological activity, and then the biological active carbon can be directly put into sewage.
Example 1
A biological activated carbon using ficus microcarpa leaves cultured by high-sugar wastewater as a carbon source carrier comprises the following specific contents:
(1) preparation of porous defect-rich carbon carrier with ficus microcarpa leaves as carbon source
1) Placing 90g of mixed alkali of NaOH and KOH in a liner of a 200 ml reaction kettle, and melting at 166-DEG C for 2h to obtain clear molten alkali liquor;
2) adding 100g of agricultural and forestry waste-ficus microcarpa leaves as a raw material into clear molten alkali liquor to react for 8 hours to generate a carbon precursor material;
3) washing the carbon precursor material with water and absolute ethyl alcohol in sequence, and drying at 60 ℃ for 12h to obtain a powdered carbon precursor;
4) calcining the powdered carbon precursor in a nitrogen atmosphere at 500 ℃ for 2h to obtain a porous defect-rich carbon powder carrier material;
2. adding 10g of porous defect-rich carbon carrier into 100 ml of high-sugar wastewater to prepare a culture solution; candida utilis (A), (B) and (C)Candida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Saccharopolyspora fuliginosa (A), (B), (C)Saccharopolyspora hirsuta) Acidocella arborescens, Acidocella spZoogloea ramigera) The activated bacteria liquid is mixed according to the mass ratio of 4:3:2:1, the mixed bacteria liquid is inoculated into the culture liquid according to the inoculation amount of 4wt percent, after the mixed bacteria liquid is cultured for 12 hours under the conditions of 30 ℃ and 150 rpm, the bacillus coagulans (C) and (C) are added into the culture liquidPaenibacillus curdlanolyticus) Bacillus brevis (B), (B)Brevibacillus parabrevis) Planktonic coccobacillus (A) and (B)Sphaerotilus natans) The activated bacterium solution is mixed according to the mass ratio of 3:3:4, the mixed bacterium solution is inoculated into the culture solution according to the inoculation amount of 1.5wt%, and the culture solution is cultured for 21 hours under the conditions of 30 ℃ and 150 rpm to formMixing the bacteria liquid, finally centrifugally separating out the carbon carrier, and drying in a constant temperature box at 20 ℃ to obtain the water purifying agent.
The effective viable count on the carbon carrier is 6.7 multiplied by 108～7.8×108CFU/g; the biological activated carbon preserved at the temperature of 4 ℃ is placed at normal temperature for 10 hours before use to recover the biological activity, and then the biological activated carbon can be directly put into sewage.
Example 2
A biological activated carbon using ficus microcarpa leaves cultured by high-sugar wastewater as a carbon source carrier comprises the following specific contents:
(1) preparation of porous defect-rich carbon carrier with ficus microcarpa leaves as carbon source
1) Putting 90g of mixed alkali of NaOH and KOH into a 200 ml reaction kettle inner container, and melting for 2 hours at 165 ℃ to obtain clear molten alkali liquor;
2) adding 100g of agricultural and forestry waste-ficus microcarpa leaves as a raw material into clear molten alkali liquor for reacting for 4 hours to generate a carbon precursor material;
3) washing the carbon precursor material with water and absolute ethyl alcohol in sequence, and drying at 60 ℃ for 12h to obtain a powdered carbon precursor;
4) calcining the powdered carbon precursor in a nitrogen atmosphere at 500 ℃ for 2h to obtain a porous defect-rich carbon powder carrier material;
2. adding 10g of porous defect-rich carbon carrier into 50ml of high-sugar wastewater to prepare a culture solution; candida utilis (A), (B) and (C)Candida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Saccharopolyspora fuliginosa (A), (B), (C)Saccharopolyspora hirsuta) Acidocella arborescens, Acidocella spZoogloea ramigera) The activated bacteria liquid is mixed according to the mass ratio of 4:3:2:1, the mixed bacteria liquid is inoculated into the culture liquid according to the inoculation amount of 3wt percent, after the mixed bacteria liquid is cultured for 12 hours under the conditions of 30 ℃ and 150 rpm, the bacillus coagulans (C) and (C) are added into the culture liquidPaenibacillus curdlanolyticus) Bacillus brevis (B), (B)Brevibacillus parabrevis) Planktonic coccobacillus (A) and (B)Sphaerotilus natans) The activated bacteria liquid is mixed according to the mass ratio of 3:3:4, the mixed bacteria liquid is inoculated into the culture liquid according to the proportion of 1wt percent of the inoculation amount, the mixed bacteria liquid is cultured for 20 hours under the conditions of 30 ℃ and 150 rpm, and finally, the mixed bacteria liquid is centrifugally separatedAnd (3) drying the carbon carrier in a constant temperature box at 25 ℃ to obtain the water purifying agent.
The effective viable count on the carbon carrier is 6.4 multiplied by 108～7.3×108CFU/g; the biological active carbon preserved at the temperature of 4 ℃ is placed at the normal temperature for 8 hours before use to recover the biological activity, and then the biological active carbon can be directly put into sewage.
Example 3
A biological activated carbon using ficus microcarpa leaves cultured by high-sugar wastewater as a carbon source carrier comprises the following specific contents:
(1) preparation of porous defect-rich carbon carrier with ficus microcarpa leaves as carbon source
1) Putting 90g of mixed alkali of NaOH and KOH into a 200 ml inner container of a reaction kettle, and melting for 2 hours at 200 ℃ to obtain clear molten alkali liquor;
2) adding 100g of agricultural and forestry waste-ficus microcarpa leaves as a raw material into clear molten alkali liquor to react for 12 hours to generate a carbon precursor material;
3) washing the carbon precursor material with water and absolute ethyl alcohol in sequence, and drying at 60 ℃ for 12h to obtain a powdered carbon precursor;
4) calcining the powdered carbon precursor in a nitrogen atmosphere at 500 ℃ for 2h to obtain a porous defect-rich carbon powder carrier material;
2. adding 10g of porous defect-rich carbon carrier into 150 ml of high-sugar wastewater to prepare a culture solution; candida utilis (A), (B) and (C)Candida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Saccharopolyspora fuliginosa (A), (B), (C)Saccharopolyspora hirsuta) Acidocella arborescens, Acidocella spZoogloea ramigera) The activated bacterium solution of (2) is mixed according to the mass ratio of 4:3:2:1, the mixed bacterium solution is inoculated into the culture solution according to the inoculation amount of 5wt%, the culture solution is cultured for 12 hours under the conditions of 30 ℃ and 150 rpm, and then the bacillus coagulans (B) is addedPaenibacillus curdlanolyticus) Bacillus brevis (B), (B)Brevibacillus parabrevis) Planktonic coccobacillus (A) and (B)Sphaerotilus natans) The activated bacteria liquid is mixed according to the mass ratio of 3:3:4, the mixed bacteria liquid is inoculated into the culture liquid according to the inoculation amount of 2wt%, the mixed bacteria liquid is cultured for 22 hours under the conditions of 30 ℃ and 150 rpm to form mixed bacteria liquid, and finally, the carbon carrier is centrifugally separated out and placed in a constant temperature box at 30 DEG CDrying to obtain the water purifying agent.
The effective viable count on the carbon carrier is 5.4 multiplied by 108～6.8×108CFU/g; the biological activated carbon preserved at the temperature of 4 ℃ is placed at normal temperature for 12 hours before use to recover the biological activity, and then the biological activated carbon can be directly put in sewage.
Comparative example 1
Basically the same as the examples 1-3, the biological activated carbon using ficus microcarpa leaves cultured by high-sugar wastewater as carbon source carrier is prepared, the difference is only that the carbon carrier prepared in the first three steps (1) is different, and the comparative example 1 is the granular coal activated carbon carrier (the specific surface is about 1100 m) sold on the market2Per g, pore volume of 0.75 cm3/g）。
And (4) performance testing:
firstly, adjusting the pH value of the high-sugar wastewater to 7.0, and adding activated carbon carriers of different materials into the high-sugar wastewater to compare the adsorption effect. Respectively weighing 100g of active carbon carriers (granular coal active carbon A, granular coconut shell active carbon B, granular bamboo charcoal C and porous defect-rich powdered carbon D) of different materials, adding into 10L of high-sugar wastewater, and determining CODcr and BOD of untreated high-sugar wastewater after 72h5And calculating the adsorption rate of the activated carbon to each index in the high-sugar wastewater. The results are shown in Table 1-1. The experimental results show that the coal-based activated carbon in the three materials has better adsorption capacity on the high-sugar wastewater, and COD and BOD of the coal-based activated carbon5The adsorption rates of ammonia nitrogen, total phosphorus and the like are higher than those of the former two; the adsorption effect of the porous defect-rich powdered carbon in the patent is superior to A, B, C, and COD and BOD5The adsorption rates of ammonia nitrogen and total phosphorus are obviously improved, the adsorption of pollutants is promoted by the cooperation of oxygen-containing functional groups on the surface and surface defects, and the decontamination effect is superior to that of the active carbon which is sold in the market and is made of different materials.
TABLE 1-1 treatment effect of carbon carriers with different shapes on high-sugar wastewater
Taking five high-sugar wastewater tanks (10L) with the same size and water quality for testing, and adding the biological activated carbon of the embodiment 1 in the first step; secondly, the microbial water purifying agent of the embodiment 2 is added; thirdly, the microbial water purifying agent of the embodiment 3 is added; fourthly, the bacterial carbon water purifying agent in the comparative example 1 is added; the fifth group was used as a blank control without any water purifying agent.
100g of water purifying agent is respectively added into a high-sugar wastewater tank (the water quality indexes of the high-sugar wastewater are that COD is 3980mg/L, BOD 1767mg/L, ammonia nitrogen is 85mg/L, total phosphorus is 24mg/L, and the pH value is adjusted to be about 7.0 by NaOH). After 72h, water quality measurements were performed on all the high-sugar wastewater tanks. The water quality detection standard is characterized by being determined according to the discharge standard of water pollutants for sugar industry (GB 21909-.
The effect of different purifiers on the index concentration of sewage after the time of Table 1-272 h
The removal effect of different purifiers on pollutants after the time of tables 1-372 h
As can be seen from the data in the table, the water quality indexes of the examples 1 to 3 are all superior to those of the comparative example 1, and are more obviously superior to those of the control group.
Comparative example 2
The service life of the carbon material of the biological activated carbon cultured by the beef extract peptone culture medium is about 28d, while the service life of the biological activated carbon cultured by the high-sugar wastewater is about 38d, which is improved by about 35.7%.
Comparative example 3
The microbial activity of the biological activated carbon, carbon material, obtained by one-step culture was compared with that of examples 1 to 3.
The adoption of the step-by-step method is favorable for increasing the effective viable count of the microorganisms and improving the biological activity thereofThe effective viable count of the microorganism loaded on the active carbon after one-step culture is 9.6 multiplied by 107～1.4×108CFU/g, effective viable count of microorganism loaded on activated carbon after two-step culture is 5.4 × 108～8.6×108CFU/g. 100g of water purifying agent after the one-step culture and the two-step culture are respectively put into the mixed wastewater, the water quality is measured after 72 hours of treatment, and the measurement indexes and data are shown in the following tables 1-4.
TABLE 1-4 treatment effect of biological activated carbon obtained by stepwise culture on sewage
The above description is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be made by those skilled in the art without inventive work within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope defined by the claims.
Claims (6)
1. A preparation method of biological activated carbon cultured by high-sugar wastewater and using ficus microcarpa leaves as a carbon source carrier is characterized by comprising the following steps: placing the porous carbon carrier rich in surface defects into high-sugar wastewater to prepare a culture solution; then Candida utilis (A), (B) andCandida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Saccharopolyspora fuliginosa (A), (B), (C)Saccharopolyspora hirsuta) And Acinetobacter arborescens (Zoogloea ramigera) Inoculating the strain into culture solution, shake co-culturing, and inoculating Bacillus coagulans (Bacillus coagulans)Paenibacillus curdlanolyticus) Bacillus brevis (B), (B)Brevibacillus parabrevis) And Phytoplankton: (Sphaerotilus natans) Inoculating the strain into a culture solution, and performing shaking co-culture and drying to obtain biological activated carbon; the porous carbon carrier rich in surface defects is prepared by taking ficus microcarpa leaves as a raw material, placing the ficus microcarpa leaves in NaOH and KOH molten alkali liquor, and melting and ethanolWashing and calcining in nitrogen atmosphere;
the preparation method of the porous carbon carrier rich in surface defects comprises the following steps:
(1) placing 90g of mixed alkali of NaOH and KOH in an inner container of a reaction kettle of 200 ml, and placing the inner container in a reactor at 165-200 ℃ for melting for 2h to obtain clear molten alkali liquor;
(2) adding 100g of ficus microcarpa leaves serving as a raw material into clear molten alkali liquor to react for 4-12 hours to generate a carbon precursor material;
(3) washing the carbon precursor material with water and absolute ethyl alcohol in sequence, and drying at 60 ℃ for 12h to obtain a powdered carbon precursor;
(4) and calcining the powder carbon precursor in a nitrogen atmosphere at 500 ℃ for 2h to obtain the porous surface defect-rich carbon powder carrier material.
2. The method for preparing biological activated carbon cultured by high-sugar wastewater and using ficus microcarpa leaves as carbon source carriers according to claim 1, is characterized by comprising the following steps: in the mixed alkali in the step (1), the mass ratio of NaOH to KOH is 46.5: 43.5.
3. The method for preparing biological activated carbon cultured by high-sugar wastewater and using ficus microcarpa leaves as carbon source carriers according to claim 1, is characterized by comprising the following steps: the method comprises the following specific steps:
1) adding 10g of porous carbon carrier rich in surface defects into 50-150 ml of high-sugar wastewater to prepare a culture solution; candida utilis (A. utilis: (A. utilis))Candida utilis) Bacillus subtilis preparation (B)Bacillus subtilis) Saccharopolyspora fuliginosa (A), (B), (C)Saccharopolyspora hirsuta) And Acinetobacter arborescens (Zoogloea ramigera) The activated bacteria liquid is mixed according to the mass ratio of 4:3:2:1, then the mixed bacteria liquid is inoculated into the culture solution according to the inoculation amount of 3wt% -5 wt%, and the culture is carried out for 12h under the conditions of 30 ℃ and 150 rpm;
2) then the bacillus coagulans (B) is addedPaenibacillus curdlanolyticus) Bacillus brevis (B), (B)Brevibacillus parabrevis) And Phytoplankton: (Sphaerotilus natans) Mixing the activated bacteria liquid according to the mass ratio of 3:3:4, and then mixingInoculating the bacterial mixture into the culture solution according to the inoculation amount of 1-2 wt%, culturing at 30 ℃ and 150 rpm for 20-22h, and finally centrifugally separating out the carbon carrier and placing in a thermostat at 20-30 ℃ for drying to obtain the water purifying agent.
4. The method for preparing biological activated carbon by using ficus microcarpa leaves as a carbon source carrier according to claim 1 is characterized in that: the indexes of the high-sugar wastewater are as follows: COD 3000-5000mg/L, BOD 2000-3000 mg/L, SS 600-800 mg/L, ammonia nitrogen 100-150 mg/L, total phosphorus 8-15 mg/L pH 4.0-6.0; before the high-sugar wastewater is used, the pH value of the high-sugar wastewater needs to be adjusted to 7.2 +/-0.2 and then 0.56kg/cm2And sterilizing at 112.6 deg.C for 20 min.
5. A biological activated carbon produced by the production method according to claim 1, characterized in that: the effective viable count on the porous carbon carrier rich in surface defects is 5.4 multiplied by 108～7.8×108CFU/g。
6. Use of the biological activated carbon of claim 5 in the treatment of high-sugar wastewater.
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