CN107435054A - Erythrite and intracellular grease are prepared by primary raw material coproduction of gutter oil - Google Patents
Erythrite and intracellular grease are prepared by primary raw material coproduction of gutter oil Download PDFInfo
- Publication number
- CN107435054A CN107435054A CN201710531895.5A CN201710531895A CN107435054A CN 107435054 A CN107435054 A CN 107435054A CN 201710531895 A CN201710531895 A CN 201710531895A CN 107435054 A CN107435054 A CN 107435054A
- Authority
- CN
- China
- Prior art keywords
- erythrite
- gutter oil
- grease
- addition
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to prepare erythrite and intracellular grease by primary raw material coproduction of gutter oil, sole carbon source in using gutter oil as culture medium, it is fermented bacterium using Yarrowia lipolytica Yarrowia lipolytica, by microbial method, realizes the efficient output of erythrite and intracellular grease.For gutter oil as sole carbon source, addition in the medium is 10 ~ 100 g/L, preferably 30 g/L, the present invention realizes product from clastotype, by centrifugation can two kinds of products of initial gross separation, simplify the separation and extraction of product, be adapted to large-scale industrial treatment pattern.Meaning of the present invention is：The Efficient Conversion of gutter oil is realized, erythrite yield significantly improves, while obtains microbial grease, and the gutter oil conversion output value significantly improves.
Description
Technical field
The present invention relates to gutter oil to utilize technical field, more particularly to prepares erythrite by primary raw material coproduction of gutter oil
With intracellular grease.
Background technology
In recent years, Chinese " gutter oil " event emerges in an endless stream, and the situation that dining table is flowed back on gutter oil repeatedly occurs, order
People talk gutter oil discoloration, it has also become prominent food-safety problem.The Taiwan gutter oil event of 2014 is even more by gutter oil
Problem has pushed the teeth of the storm to, and many enterprises are involved.And the underground industrial chain that gutter oil is maximum as one, it is to us
The influence of life just grows in intensity.As the development of science and technology and the correct guiding of national policy are with encouraging, the utilization of gutter oil is asked
Topic has been brought into schedule and developed rapidly.
The gutter oil of ordinary meaning refers to the cooking Residual oil of catering trade, leftovers（It is generally called swill）Flowed into etc. grease-contained waste water
Sewer, the grease that can not be eaten again caused by after oil water separator or oil interceptor separation.Advised with the catering trade in China
Mould expands day by day, and the waste oil discharged in food and drink waste water is increasing, not only causes the serious pollution of urban environment, and produce
Waste oil illegally reclaims the illegal phenomenon for refining backflow food products market.Gutter oil contains a variety of poisonous and harmful elements, heavy metal
Material and trans-fatty acid, there is very strong carcinogenicity.But due to cheap, gutter oil is used for the phenomenon of illegal approach repeatly
Prohibit more than.In recent years, the extensive concern of researcher how has rationally efficiently been caused using gutter oil, wherein utilizing gutter oil
Prepare biodiesel and glycerine has turned into the Main Means of processing gutter oil.The U.S., European Union and Japan and other countries have passed through country
The means of support improve the unit take-back cost of gutter oil, it is flowed to the production of bio-fuel, value, which exceeds well over, flows back to food
Market.China produces big country as gutter oil, and its annual production is about 10,000,000 tons, and total amount is huge.
The utilization of gutter oil at present, which focuses primarily upon, is converted into biodiesel, but gutter oil is to the conversion face of biodiesel
Face very big problem, as accessory substance is excessive, the higher grade of cost by government finance, it is necessary to subsidize to maintain, therefore gutter oil converts
Problem is not yet solved at all.The dominant catalyst of gutter oil conversion process has two kinds of chemical method and biological enzyme.Currentization
It is more to learn catalysis method research, such as in Fuel Processing Technology（2011,92（1）：83-91）The Fredy of publication
" the Continulous transesteritication of biodiesel in a helicoidal that A and Joan S write
In the texts of reactor using recycled oil " one, using NaOH as catalyst, Preparation of biodiesel from waste oils is catalyzed；Liu Zheng
Et al. in New Chemical Materials（2015,39（8）：52-56）" gutter oil recycling key skill in alcohol-based fuel of publication
In the text of art research " one, gutter oil recycling key technology in alcohol-based fuel is have studied, using KOH as catalyst, utilizes ester
Exchange reaction prepares biodiesel.Chemical transformation cost is more cheap, but easily causes environmental pollution.Simultaneously because gutter oil comes
Source is differed, complicated component, would generally be brought during biodiesel synthesis along with the generation of by-product glycerin to product separation
It is difficult；Biological enzyme mild condition, adaptability to raw material is good, and accessory substance is easily separated.Such as Tan is in Biotechnology
Advances（2010,28（5）：628-634）The Biodiesel production with immobilized delivered
lipase：Mentioned in A review with lipase-catalyzed Preparation of biodiesel from waste oils, but this method catalytic efficiency is low, and price is held high
It is expensive, it is unsuitable for mass producing.
Erythrite is a kind of natural healthy sugar alcohol, white crystalline body.Erythrite heat and glycemic index are extremely low, suitable for sugar
Urine disease and cardiovascular patient are eaten.It is safe sweetener that erythrite is proved in animal toxicology and clinical research.Base
In above attribute, erythrite is widely used in specific function food processing, and demand is growing.By 2011, the world was red
Moss alcohol annual requirement is more than 46000 tons.Cause its fermentation costs higher using glucose as raw material because erythrite fermentation is more,
Costly, the market price per ton reaches 4000 dollars to price.Intracellular grease is the degree of unsaturation of accumulation in extracellular microbial
Higher grease, it is the favorable substitutes of traditional edible oil, so as to alleviate animal and plant fat present situation in short supply.In addition, intracellular is oily
Fat is alternatively arranged as biodiesel raw material.
The content of the invention
It is an object of the invention to provide a kind of new processing method to prepare red moss by primary raw material coproduction of gutter oil
Alcohol and intracellular grease, gutter oil processing cost is reduced, while increase the conversion output value of gutter oil.By medium component optimization and
Trace metal ion regulation and control Product formation and coproduction efficiency are added, the gutter oil conversion output value is further improved.Meanwhile with trench
Oil is fermenting raw materials erythrite, can significantly reduce erythrite fermentation costs.
The invention provides erythrite and intracellular grease is prepared by primary raw material coproduction of gutter oil, training is used as using gutter oil
The sole carbon source in base is supported, utilizes Yarrowia lipolyticaYarrowia lipolyticaFor fermented bacterium,By microbial method,
Realize the efficient output of erythrite and intracellular grease.
For the gutter oil as sole carbon source, addition in the medium is 10 ~ 100 g/L, preferably 30 g/L；
It is described that the strain utilized in erythrite and intracellular grease is prepared to solve fat Ye Shi ferment by primary raw material coproduction using gutter oil
It is femaleYarrowia lipolytica, purchased from China General Microbiological culture presevation administrative center, bacterium numbering is
CGMCC2.1207。
Regulate and control fermentation by adding copper ion with manganese ion in the culture medium, increase Product yields；
The copper ion is provided by copper nitrate, and the amount of addition is 1 ~ 10 mg/L, preferably 4 mg/L, is added in culture medium appropriate
After copper ion, erythrite yield is improved to 0.63 g/g carbon sources by 0.54 original g/g carbon sources, and intracellular grease yield is by 0.47
G/g dry cell weights are improved to 0.51g/g dry cell weights；
The manganese ion is provided by manganese sulfate, and addition concentration is 1 ~ 15 mg/L, preferably 10 mg/L, and erythrite yield is by 0.63
G/g carbon sources improve to 0.75 g/g carbon sources, intracellular grease yield and are improved to 0.54 g/g cells and done by 0.51 g/g dry cell weights
Weight；
It is described medium component used is prepared in erythrite and intracellular grease by primary raw material coproduction of gutter oil to be：Gutter oil
As sole carbon source, addition in the medium is 10 ~ 100 g/L, preferably 30 g/L；Osmotic pressure regulator is chlorination
Sodium, addition concentration are 0 ~ 100 g/L, preferably 60 g/L；Fermentation nitrogen source is corn steep liquor, and addition is 1 ~ 5 g/L, preferably 2
g/L；Phosphate is potassium dihydrogen phosphate, and addition is 1 ~ 5 g/L, preferably 1 g/L；Magnesium ion is provided by magnesium sulfate, addition
For 1 ~ 5 g/L, preferably 2 g/L；The g/L of dusty yeast addition 0.25；Copper ion is provided by copper nitrate, and the amount of addition is 1 ~ 10
Mg/L, preferably 4 mg/L；Manganese ion is provided by manganese sulfate, and addition concentration is 1 ~ 15 mg/L, preferably 10 mg/L.
The present invention is for the problem of traditional gutter oil conversion process is complicated, cost is higher, from microbial fermentation angle, structure
Gutter oil is the fermentation system of raw material coproduction erythrite and intracellular grease, reduces gutter oil processing cost, while the warp of product
The Ji output value significantly improves；By medium component optimization and metal ion addition, make product generation and transport regulation and control more
Accurately effectively, to improve Product yields；Using corn steep liquor as the source of fermentation nitrogen source and other nutriments, further reduce
Fermentation costs；Obtained in the present invention tunning erythrite and intracellular grease can natural separation, simplify follow-up production
Thing extraction process.
Embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example only part of the embodiment of the present invention, rather than whole embodiments.It is common based on the embodiment in the present invention, this area
The every other embodiment that technical staff is obtained under the premise of creative work is not made, belong to the model that the present invention protects
Enclose.
In order to become apparent from introducing in detail the embodiment of the present invention provided prepared using gutter oil as primary raw material coproduction it is red
Moss alcohol and intracellular grease, will specifically it illustrate below.
Embodiment 1
A kind of method for preparing erythrite and intracellular grease using gutter oil coproduction, including：Bacterial strain activation, seed culture, fermentation
Four coproduction, product separation and purifying steps；
1）By strain by line in the glycerol tube that freezes to YPD flat boards, cultivation temperature is 28 DEG C, activation culture 48h, for seed
Inoculation and fungi preservation, strain preserve in YPD inclined-planes；
2）Conditions of flask fermentation is：Shaking culture volume is 50 mL, and inoculum concentration is 1 × 106Individual cell/L culture mediums, culture temperature
26 DEG C of degree, cultivates the rpm of rotating speed 150, and incubation time is 3 days；
3）Ferment tank condition is：Fermentation system 5 L, fermentation temperature 28oC, rotating speed are 250 rpm, throughput 1.0
L/min/L volume of culture.Thalline access amount is 1.0 ' 108Individual yeast cells/L culture mediums.Fermentation time is 7 days；
4）In the method for gutter oil coproduction erythrite and microbial grease of fermenting, two kinds of products of erythrite and intracellular grease it is preliminary
Separation uses centrifugal process；
5）In the method for fermentation gutter oil coproduction erythrite and microbial grease, conventionally extract respectively in zymotic fluid
Erythrite and intracellular grease.
The strain utilized in the method for erythrite and intracellular grease that prepared using gutter oil coproduction is solution fat Ye Shi
YeastYarrowia lipolytica。
Culture medium basis used is in the method that erythrite and intracellular grease are prepared using gutter oil coproduction：
Gutter oil is as sole carbon source, and addition in the medium is 10 g/L, and osmotic pressure regulator is sodium chloride, and addition concentration is
1 g/L, fermentation nitrogen source are corn steep liquor, and addition is 1 g/L, and phosphate is potassium dihydrogen phosphate, and addition is 1 g/L, magnesium ion
There is provided by magnesium sulfate, addition is 1 g/L, the g/L of dusty yeast addition 0.25, and copper ion is provided by copper nitrate, and the amount of addition is
1 mg/L, manganese ion are provided by manganese sulfate, and addition concentration is 1 mg/L.
Embodiment 2
A kind of method for preparing erythrite and intracellular grease using gutter oil coproduction, including：Bacterial strain activation, seed culture, fermentation
Four coproduction, product separation and purifying steps；
1）By strain by line in the glycerol tube that freezes to YPD flat boards, cultivation temperature is 29 DEG C, the h of activation culture 52, for planting
Son inoculation and fungi preservation, strain preserve in YPD inclined-planes；
2）Conditions of flask fermentation is：Shaking culture volume is 50 mL, and inoculum concentration is 1 × 108Individual cell/L culture mediums, culture temperature
30 DEG C of degree, cultivates the rpm of rotating speed 200, and incubation time is 7 days；
3）Ferment tank condition is：Fermentation system 5 L, fermentation temperature 28oC, rotating speed are 250 rpm, throughput 1.0
L/min/L volume of culture.Thalline access amount is 1.0 ' 108Individual yeast cells/L culture mediums.Fermentation time is 7 days；
4）In the method for gutter oil coproduction erythrite and microbial grease of fermenting, two kinds of products of erythrite and intracellular grease it is preliminary
Separation uses centrifugal process；
5）In the method for fermentation gutter oil coproduction erythrite and microbial grease, conventionally extract respectively in zymotic fluid
Erythrite and intracellular grease.
The strain utilized in the method for erythrite and intracellular grease that prepared using gutter oil coproduction is solution fat
Family name's yeastYarrowia lipolytica。
Culture medium basis used is in the method that erythrite and intracellular grease are prepared using gutter oil coproduction：
Gutter oil is as sole carbon source, and addition in the medium is 50 g/L, and osmotic pressure regulator is sodium chloride, and addition concentration is
50 g/L, fermentation nitrogen source are corn steep liquor, and addition is 2.5 g/L, and phosphate is potassium dihydrogen phosphate, and addition is 2.5 g/L, magnesium
Ion is provided by magnesium sulfate, and addition is 2.5 g/L, the g/L of dusty yeast addition 0.25, and copper ion is provided by copper nitrate, is added
Amount be 5 mg/L, manganese ion is provided by manganese sulfate, and addition concentration is 10 mg/L.
Embodiment 3
A kind of method for preparing erythrite and intracellular grease using gutter oil coproduction, including：Bacterial strain activation, seed culture, fermentation
Four coproduction, product separation and purifying steps；
1）By strain by line in the glycerol tube that freezes to YPD flat boards, cultivation temperature is 30 DEG C, the h of activation culture 72, for planting
Son inoculation and fungi preservation, strain preserve in YPD inclined-planes；
2）Conditions of flask fermentation is：Shaking culture volume is 50 mL, and inoculum concentration is 1 × 109Individual cell/L culture mediums, culture temperature
36 DEG C of degree, cultivates the rpm of rotating speed 220, and incubation time is 10 days；
3）Ferment tank condition is：Fermentation system 5 L, fermentation temperature 28oC, rotating speed are 250 rpm, throughput 1.0
L/min/L volume of culture.Thalline access amount is 1.0 ' 108Individual yeast cells/L culture mediums.Fermentation time is 7 days；
4）In the method for gutter oil coproduction erythrite and microbial grease of fermenting, two kinds of products of erythrite and intracellular grease it is preliminary
Separation uses centrifugal process；
5）In the method for fermentation gutter oil coproduction erythrite and microbial grease, conventionally extract respectively in zymotic fluid
Erythrite and intracellular grease.
The strain utilized in the method for erythrite and intracellular grease that prepared using gutter oil coproduction is solution fat
Family name's yeastYarrowia lipolytica。
Culture medium basis used is in the method that erythrite and intracellular grease are prepared using gutter oil coproduction：
For gutter oil as sole carbon source, addition in the medium is 100 g/L, and osmotic pressure regulator is sodium chloride, adds concentration
For 100 g/L, fermentation nitrogen source is corn steep liquor, and addition is 5 g/L, and phosphate is potassium dihydrogen phosphate, and addition is 5 g/L, magnesium
Ion is provided by magnesium sulfate, and addition is 5 g/L, the g/L of dusty yeast addition 0.25, and copper ion is provided by copper nitrate, addition
Measure and provided for 10 mg/L, manganese ion by manganese sulfate, addition concentration is 15 mg/L.
Claims (8)
1. prepare erythrite and intracellular grease by primary raw material coproduction of gutter oil, using gutter oil as culture medium in it is unique
Carbon source, it is fermented bacterium using Yarrowia lipolytica Yarrowia lipolytica, by microbial method, realizes red moss
The efficient output of alcohol and intracellular grease.
2. erythrite and intracellular grease are prepared by primary raw material coproduction of gutter oil according to claim 1, its feature
It is, for the gutter oil as sole carbon source, addition in the medium is 10 ~ 100g/L, preferably 30 g/L.
3. erythrite and intracellular grease are prepared by primary raw material coproduction of gutter oil according to claim 1, its feature
It is, it is described that the strain utilized in erythrite and intracellular grease is prepared to solve fat Ye Shi by primary raw material coproduction using gutter oil
Yeast Yarrowia lipolytica, purchased from China General Microbiological culture presevation administrative center, bacterium numbering is
CGMCC2.1207。
4. erythrite and intracellular grease are prepared by primary raw material coproduction of gutter oil according to claim 1, its feature
It is, regulates and controls fermentation by adding copper ion with manganese ion in the culture medium, increase Product yields.
5. regulating and controlling fermentation by adding copper ion with manganese ion according in the culture medium described in claim 4, its feature exists
In the copper ion is provided by copper nitrate, and the amount of addition is 1 ~ 10 mg/L, preferably 4 mg/L, is added in culture medium appropriate
After copper ion, erythrite yield is improved to 0.63 g/g carbon sources by 0.54 original g/g carbon sources, intracellular grease yield by
0.47 g/g dry cell weights are improved to 0.51g/g dry cell weights.
6. according to described in claim 4 to regulate and control fermentation, its feature by adding copper ion and manganese ion in culture medium
Be, the manganese ion is provided by manganese sulfate, and addition concentration is 1 ~ 15 mg/L, preferably 10 mg/L, erythrite yield by
0.63 g/g carbon sources are improved to 0.75 g/g carbon sources, intracellular grease yield and improved by 0.51 g/g dry cell weights to 0.54
G/g dry cell weights.
7. erythrite and intracellular grease are prepared by primary raw material coproduction of gutter oil according to claim 1, its feature
It is, medium component is：For gutter oil as sole carbon source, addition in the medium is 10 ~ 100 g/L, is preferably
30 g/L；Osmotic pressure regulator is sodium chloride, and addition concentration is 0 ~ 100 g/L, preferably 60 g/L；Fermentation nitrogen source is corn
Slurry, addition is 1 ~ 5 g/L, preferably 2 g/L；Phosphate is potassium dihydrogen phosphate, and addition is 1 ~ 5 g/L, preferably 1
g/L；Magnesium ion is provided by magnesium sulfate, and addition is 1 ~ 5 g/L, preferably 2 g/L；The g/L of dusty yeast addition 0.25；Copper
Ion is provided by copper nitrate, and the amount of addition is 1 ~ 10 mg/L, preferably 4 mg/L；Manganese ion is provided by manganese sulfate, and addition is dense
Spend for 1 ~ 15mg/L, preferably 10 mg/L.
A kind of 8. side that erythrite and intracellular grease are prepared using gutter oil as primary raw material coproduction using described in claim 1
Method, it is characterised in that including：
Four bacterial strain activation, seed culture, fermentation coproduction, product separation and purifying steps；
1）By strain by line in the glycerol tube that freezes to YPD flat boards, cultivation temperature is 28 ~ 30 DEG C, activation culture 48 ~ 72
H, for seed inoculation and fungi preservation, strain preserves in YPD inclined-planes；
2）Conditions of flask fermentation is：Shaking culture volume is 50 mL, and inoculum concentration is 1 × 106 ~ 1 × 109 cells/L cultures
Base, 26 ~ 36 DEG C of cultivation temperature, cultivate the rpm of rotating speed 150 ~ 220, preferably 200 rpm；Incubation time is 3 ~ 10 days；
3）Ferment tank condition is：The L of fermentation system 5, fermentation temperature 28oC, rotating speed is 250 rpm, and throughput is
1.0 l/min/l volume of culture, thalline access amount are 1.0 × 108 yeast cells/L culture mediums, fermentation time 7
My god；
4）In the method for gutter oil coproduction erythrite and microbial grease of fermenting, two kinds of products of erythrite and intracellular grease it is preliminary
Separation uses centrifugal process；
5）In the method for fermentation gutter oil coproduction erythrite and microbial grease, conventionally extract respectively in zymotic fluid
Erythrite and intracellular grease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710531895.5A CN107435054A (en) | 2017-07-03 | 2017-07-03 | Erythrite and intracellular grease are prepared by primary raw material coproduction of gutter oil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710531895.5A CN107435054A (en) | 2017-07-03 | 2017-07-03 | Erythrite and intracellular grease are prepared by primary raw material coproduction of gutter oil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107435054A true CN107435054A (en) | 2017-12-05 |
Family
ID=60459798
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710531895.5A Pending CN107435054A (en) | 2017-07-03 | 2017-07-03 | Erythrite and intracellular grease are prepared by primary raw material coproduction of gutter oil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107435054A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108456700A (en) * | 2018-04-16 | 2018-08-28 | 昆明理工大学 | A method of improving grease in microalgae using give up mash and magnesium ion of molasses |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL410179A1 (en) * | 2014-11-19 | 2015-10-12 | Uniwersytet Przyrodniczy we Wrocławiu | Method for obtaining erythritol |
-
2017
- 2017-07-03 CN CN201710531895.5A patent/CN107435054A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL410179A1 (en) * | 2014-11-19 | 2015-10-12 | Uniwersytet Przyrodniczy we Wrocławiu | Method for obtaining erythritol |
Non-Patent Citations (4)
| Title |
|---|
| LIU XIAOYAN ET AL.: "A cost-effective process for the coproduction of erythritol and lipase with Yarrowia lipolytica M53 M53from waste cooking oil", 《FOOD AND BIOPRODUCTS PROCESSING》 * |
| LUDWIKA TOMASZEWSKA ET AL.: "Mineral Supplementation Increases Erythrose Reductase Activity in Erythritol Biosynthesis from Glycerol by Yarrowia lipolytica", 《APPL BIOCHEM BIOTECHNOL》 * |
| 李永红等: "圆红冬孢酵母菌发酵产油脂培养基及发酵条件的优化研究", 《生物工程学报》 * |
| 柯智健等: "解脂耶罗维亚酵母菌利用地沟油联产柠檬酸和胞内油脂", 《食品与发酵工业》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108456700A (en) * | 2018-04-16 | 2018-08-28 | 昆明理工大学 | A method of improving grease in microalgae using give up mash and magnesium ion of molasses |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wu et al. | Bioethanol production from taro waste using thermo-tolerant yeast Kluyveromyces marxianus K21 | |
| Liu et al. | Refining bioethanol from stalk juice of sweet sorghum by immobilized yeast fermentation | |
| CN103923948B (en) | It is a kind of to prepare ethyl alcohol, the co-production of biogas and biodiesel using organic waste | |
| WO2009058471A1 (en) | Systems and processes for cellulosic ethanol production | |
| CN101638673B (en) | Method for manufacturing alcohol by utilizing fermentation of plant straws | |
| CN101864362A (en) | Compound microbial bacterial preparation and application thereof | |
| Smachetti et al. | Microalgal biomass as an alternative source of sugars for the production of bioethanol | |
| Lamb et al. | Fermentative bioethanol production using enzymatically hydrolysed Saccharina latissima | |
| de Vasconcelos | Ethanol fermentation | |
| CN105274178B (en) | A kind of composite bacteria agent that lignite ex situ is produced the method for methane coproduction humic acid and wherein applied | |
| CN104955956A (en) | A fermentation process | |
| CN103103128A (en) | Method for high efficiency enrichment culture of microalgae | |
| Sudhakar et al. | Algae as a sustainable and renewable bioresource for bio-fuel production | |
| CN101608192A (en) | A kind of method of utilizing corn cob to produce Succinic Acid | |
| CN104388484B (en) | A kind of method that microbial grease is produced using volatile fatty acid as fermenting raw materials | |
| CN105349588B (en) | The method for producing docosahexaenoic acid using schizochytrium limacinum | |
| CN102061262A (en) | Oleaginous microorganism culturing method | |
| CN101445810A (en) | Method for preparing hydrogen by fermenting biologically pretreated straw | |
| CN106893682B (en) | Method for expanding culture of saccharomycetes by using liquefied mash and application of saccharomycetes and method for fermenting ethanol | |
| CN101851614A (en) | Process for improving fermentation conversion rate of enzyme preparation | |
| CN107435054A (en) | Erythrite and intracellular grease are prepared by primary raw material coproduction of gutter oil | |
| CN103966273B (en) | A kind of dino flagellate fermenting and producing DHA method | |
| CN105624213B (en) | A method of 2,3- butanediol is produced using microalgae for raw material | |
| CN105624212B (en) | A method of 2,3- butanediol is produced by raw material of microalgae | |
| CN106755148A (en) | A kind of method that mixed fungus fermentation " one kettle way " based on the orientation regulation and control of carbon nitrogen stream produces microbial grease |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171205 |