CN107418995B - A kind of ellagic acid and preparation method thereof of granatanine liquid state fermentation preparation - Google Patents
A kind of ellagic acid and preparation method thereof of granatanine liquid state fermentation preparation Download PDFInfo
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Abstract
A kind of ellagic acid and preparation method thereof of granatanine liquid state fermentation preparation, belongs to cosmetics, drug, health product technology field.The present invention directly extracts concentrated aqueous solution as fermenting substrate tank liquid state fermentation using granatanine；Using black-koji mould (Aspergillus niger), Penicillium notatum (PencicillinG) and Escherichia coli (Escherichia.Coli) 3 kinds of bacterium mix according to a certain percentage, and prepare immobilization micelle using sodium carboxymethylcellulose, sodium alginate and diatomite, it is used multiple times, improves the utilization efficiency of strain；Complementary effect can be formed using 3 kinds of bacterium mixed fermentations, keeps ellagic acid transformation efficiency higher；The technique fermentation condition is mild, greatly reduces the consumption of chemical substance, and environmental pollution is small, is more suitable for producing on a large scale.
Description
Technical field
Prepare ellagic acid and preparation method thereof the present invention relates to a kind of granatanine liquid state fermentation, belong to cosmetics, drug,
The technical fields such as health care product.
Background technique
Ellagic acid (ellagic acid), molecular formula C14H6O8, it is the dimerization derivative of gallic acid, is that one kind is more
Phenol dilactone.Ellagic acid sterling is a kind of yellow needle-like crystals, is slightly soluble in water, alcohol, is dissolved in hot methanol, alkali and pyridine.Its molecule
Structure is as follows:
Ellagic acid is to be widely present in various nuts, the natural polyphenol class compound in fruit, especially with content in granatum
It is abundant.But free state is less, is existed with condensed forms (such as Ellagitannins, glycosides).The plantation of China pomegranate
Area is big, and granatum is resourceful, most of all to be discarded in addition to small part is used for Chinese medicine, causes very big wave
Take.Ellagic acid is produced by raw material of granatum, production cost is low, and added value of product is high, moreover it is possible to turn waste into wealth.
The ability that nearly 20 years research discovery ellagic acids have stronger anti peroxidation of lipid, capture free radical；It can press down
The metabolic activity of carcinogen processed releases carcinogenic toxicity；It is inhibited to human immunodeficiency virus；Also there are stomach and intestine
Dynamics and urogenital kinetics function, and there is blood coagulation, decompression, sedation.Therefore, it is paid close attention to by people.
Ellagic acid can be obtained by extraction, chemical synthesis and natural products edman degradation Edman, and specific preparation method mainly has
It is several below:
The first is that ellagic acid is extracted from plants.In vegetable materials such as chestnut spiny involucre, raspberry, pomegranate and sorghum kind shells
In containing ellagic acid (about 1%) free on a small quantity, the ellagic acid of high-purity can be prepared with this method.But due to cost of material is higher,
And ellagic acid content is low, is producing upper no practical significance.
It is for second the oxidation polymerization by gallic acid (or its ester).Synthesizing precursor substance --- gallic acid (ester) needs
Oxidation polymerization ellagic acid is produced under the action of enzyme.The yield of this method ellagic acid is 20%~30%.But gallic acid (ester)
Preparation process very complicated, enzyme is also required to polishing purification, and there are unknown quinones substances in synthetic product, with tan flower
The ratio of acid is 1: 2.4, brings certain difficulty to isolating and purifying for product, less at present to carry out ellagic acid with this method
Production.
The third is the technique (patent No.: 200510002081.X) that acid-hydrolysis method prepares ellagic acid, this method has higher
Yield and purity, but high concentration strong acid need to be consumed, and complete under the conditions of long-time heating, to the requirement of equipment resistance to corrosion
Very high, environmental pollution is heavier.
4th kind is presently disclosed using granatum as the non-chemical method for preparing ellagic acid of raw material.One is enzyme catalysis method
(patent No.: 200810056007.X), main process are divided into two steps: liquid state fermentation producing enzyme stage and enzymatic transformation stage, this
Method process is cumbersome, and enzymatic conversion condition requires strictly, to be difficult to control.Disclosed another method is aspergillus niger solid state fermentation
(patent No.: 201010178430.4), process is that Pomegranate Rind is placed in fermentor, and granatum is with accounting for total weight 47%
~60% initial wetting aqueous solution soaks, added with 0.1% NaCl, 0.1% MgSO in fountain solution used4With 0.5%
NH4NO3, wherein NH4NO3As the source N, 5~7,115 DEG C of 30 min of sterilizing of pH, accessed after cooling aspergillus niger (Aspergillus niger3.316) usage ratio of spore suspension, granatum and aspergillus niger be 5 g granatums add 1 mL concentration be 1 ×
1010The aspergillus niger spore suspension of a/mL.Simultaneously stationary culture is mixed, 30 DEG C ferment 5~7 days, obtain the fermentation containing ellagic acid
Product.This method uses fermentor solid state fermentation, and charging and discharging are more troublesome, and top and the bottom fermentation is uneven；It is this again
Method is fermented using only single culture, and efficiency is to be improved, these aspects have certain limitation to large-scale industrial production.
Summary of the invention
The more efficient liquid state fermentation side of ellagic acid is prepared by substrate of granatanine the object of the present invention is to provide a kind of
Method.By using granatanine extracting solution as fermentation substrate, through black-koji mould(Aspergillus niger), Penicillium notatum
(PencicillinG) with Escherichia coli (Escherichia. Coli) co-immobilization liquid fermentation method production ellagic acid, through first
High-purity ellagic acid is obtained by extraction in alcohol.This method is easy to operate, and the purity and yield of ellagic acid are higher.
The present invention uses sodium carboxymethylcellulose, seaweed using black-koji mould, Penicillium notatum and Escherichia coli as fermenting microbe
Sour sodium is fixed altogether, using granatanine extracting solution as fermentation substrate, carries out liquid state fermentation.In fermentation process, aspergillus niger, mould and
The equal syntannin enzyme of Escherichia coli is simultaneously secreted into extracellular, the Ellagitannins degradation generation tan flower in tannin enzymatic granatum
Acid, tunning are extracted and are washed purifying and obtain high-purity ellagic acid, comprising the following steps:
(1) prepared by granatanine extracting solution
Dry granatum is crushed to 40 mesh, is mentioned using 60-80 DEG C of ethanol solution reflux of 61%~70% volume fraction
It takes 3 times, the 1st addition ethanol solution is 10~15 times of Pomegranate Rind quality, extracts 2 h, is centrifugated filtrate and filter residue；
2nd 8~10 times of ethanol solution of the addition into filter residue, extracts 1.5 h, centrifuge separation；Ethanol solution is added into filter residue 3rd time
6~8 times, extract 1 h, centrifuge separation；Merge 3 filtrates, be distilled to recover ethyl alcohol, it is water-soluble to obtain granatanine extraction concentration
Liquid can be directly used for fermenting and producing.Tannin content 1%~1.5% in concentrated aqueous solution is extracted after measured.
(2) culture of fermenting microbe is fixed together
By black-koji mould, Penicillium notatum and Escherichia coli respectively in liquid complete medium in 33 DEG C of 12 h of shaken cultivation
After (80r/min), 3 kinds of culture solutions are centrifuged 12~10 min respectively at 5200~6000 r/min, spore is obtained and thallus is outstanding
Liquid dilutes bacteria suspension with sterile water, makes spore or thallus number up to 106~107A/mL, then by 3 kinds of bacteria suspensions with 3: 2: 2 body
Product ratio after mixing, then in 1: 1 ratio is mixed with the colloid aqueous solution of sodium carboxymethylcellulose and sodium alginate, colloid
The mass percent of sodium carboxymethylcellulose is 1% in aqueous solution, and the mass percent of sodium alginate is 4%, then by 1%~2%
The diatomite of 60 mesh is added in mass ratio, stirs evenly, and what then instillation was stirred continuously contains 4%CaCl2And 2%MgSO4Mixing it is water-soluble
In liquid, 2 h are impregnated, the spheric granules of 2~3 mm of diameter is made, it is spare after being washed twice with sterile water.
(3) inoculation and fermentation
Granatanine made from (1) is extracted concentrated aqueous solution and is packed into fermentor, (the NH of mass ratio 0.2% is added4)2HPO4, then immobilized bacteria micelle access fermentor, inoculum concentration is the 10%~15% of quality of fermentation broth ratio, fermentation temperature control
At 33~35 DEG C, be pumped into filtrated air from fermenter base, air mass flow be 0.4~0.6 times of fermentating liquid volume per hour, hair
Ferment 4~5 days.It is separated by filtration fermentation liquid and immobilized bacteria micelle, strain micelle can be used continuously 20 times or more, and fermentation liquid is used for
Extract ellagic acid.
(4) it extracts
Fermentation liquid containing ellagic acid obtains the precipitating containing ellagic acid by centrifuge separation, 4~6 times of volumes of the 1st addition
70 DEG C of 4 h of reflux extraction of anhydrous methanol, filtering；The anhydrous methanol reflux extraction 3 of 2nd 2~3 times of volume of addition into filter residue
H, filtering；Merge time filtrate, to being saturated, the concentrate of saturation is stood still for crystals revolving recycling methanol at room temperature, and filtering gained is solid
Body crystallization is freeze-dried up to ellagic acid product after deionized water is washed.
Essence of the invention is black-koji mould, Penicillium notatum and Escherichia coli hair using granatanine as liquid state fermentation substrate
Ferment generates tannase, and specificity is high, and the ester bond in the degradation Ellagitannins of specificity generates the degradation of granatum Ellagitannins
Ellagic acid obtains the ellagic acid of high-purity after abstraction purification.
The invention has the advantages that the present invention can form complementary effect using 3 kinds of bacterium mixed fermentations, make ellagic acid transformation efficiency
It is higher；3 kinds of bacterium mix according to a certain percentage, and prepare immobilization glue using sodium carboxymethylcellulose, sodium alginate and diatomite
Grain, increase immobilization micelle utilizes number, improves the utilization efficiency of strain.The technique fermentation condition is mild, subtracts significantly
The consumption of few chemical substance, environmental pollution is small, is more suitable for producing on a large scale.
Specific embodiment
Embodiment 1:
(1) prepared by granatanine extracting solution
Dry granatum is crushed to 40 mesh, using 72 DEG C of the ethanol solution refluxing extraction 3 times of 62% volume fraction, the 1st
Secondary ethanol solution is added and is Pomegranate Rind quality 12 times, extract 2 h, are centrifugated filtrate and filter residue；2nd time into filter residue
10 times of ethanol solution are added, 1.5 h, centrifuge separation are extracted；3rd 8 times of ethanol solution of the addition into filter residue, extracts 1 h, centrifugation
Separation；Merge 3 filtrates, be distilled to recover ethyl alcohol, obtain granatanine and extract concentrated aqueous solution, can be directly used for fermentation life
It produces.Tannin content 1%~1.5% in concentrated aqueous solution is extracted after measured.
(2) culture of fermenting microbe is fixed together
By black-koji mould, Penicillium notatum and Escherichia coli respectively in liquid complete medium in 33 DEG C of 12 h of shaken cultivation
After (80r/min), 3 kinds of culture solutions are centrifuged 12 min respectively at 5200 r/min, spore and thallus suspension are obtained, with sterile water
Bacteria suspension is diluted, makes spore or thallus number up to 106~107Then a/mL is mixed 3 kinds of bacteria suspensions with 3: 2: 2 volume ratio
It after uniformly, then is mixed with the colloid aqueous solution of sodium carboxymethylcellulose and sodium alginate in 1: 1 ratio, carboxylic in colloid aqueous solution
The mass percent of sodium carboxymethylcellulose pyce is 1%, and the mass percent of sodium alginate is 4%, then 60 mesh are added by 1%% mass ratio
Diatomite, stir evenly, then instill be stirred continuously containing 4%CaCl2And 2%MgSO4Mixed aqueous solution in, impregnate 2 h,
The spheric granules of 2~3 mm of diameter is made, it is spare after being washed twice with sterile water.
(3) inoculation and fermentation
Granatanine made from (1) is extracted concentrated aqueous solution and is packed into fermentor, (the NH of mass ratio 0.2% is added4)2HPO4, then immobilized bacteria micelle access fermentor, inoculum concentration is the 10% of quality of fermentation broth ratio, and fermentation temperature is controlled 33
DEG C, be pumped into filtrated air from fermenter base, air mass flow be 0.4 times of fermentating liquid volume per hour, ferment 5 days.Filtering point
From fermentation liquid and immobilized bacteria micelle, strain micelle can be used continuously 20 times or more, and fermentation liquid is for extracting ellagic acid.
(4) it extracts
Fermentation liquid containing ellagic acid obtains the precipitating containing ellagic acid, the nothing of 4 times of volumes of the 1st addition by centrifuge separation
70 DEG C of 4 h of reflux extraction of water methanol, filtering；3 h of anhydrous methanol reflux extraction of 2nd 3 times of volume of addition into filter residue, mistake
Filter；Merge time filtrate, to being saturated, the concentrate of saturation is stood still for crystals revolving recycling methanol at room temperature, filters obtained solid knot
Crystalline substance is freeze-dried up to ellagic acid product after deionized water is washed.
Embodiment 2:
(1) prepared by granatanine extracting solution
Dry granatum is crushed to 40 mesh, is used: 73 DEG C of the ethanol solution refluxing extraction 3 times of 65% volume fraction, the
1 addition ethanol solution is 14 times of Pomegranate Rind quality, extracts 2 h, is centrifugated filtrate and filter residue；2nd time to filter residue
Middle 9 times of addition ethanol solution, extract 1.5 h, centrifuge separation；3rd 7 times of ethanol solution of the addition into filter residue, extracts 1 h, from
Heart separation；Merge 3 filtrates, be distilled to recover ethyl alcohol, obtain granatanine and extract concentrated aqueous solution, can be directly used for fermentation life
It produces.Tannin content 1%~1.5% in concentrated aqueous solution is extracted after measured.
(2) culture of fermenting microbe is fixed together
By black-koji mould, Penicillium notatum and Escherichia coli respectively in liquid complete medium in 33 DEG C of 12 h of shaken cultivation
After (80r/min), 3 kinds of culture solutions are centrifuged 11 min respectively at 5500 r/min, spore and thallus suspension are obtained, with sterile water
Bacteria suspension is diluted, makes spore or thallus number up to 106~107Then a/mL is mixed 3 kinds of bacteria suspensions with 3: 2: 2 volume ratio
It after uniformly, then is mixed with the colloid aqueous solution of sodium carboxymethylcellulose and sodium alginate in 1: 1 ratio, carboxylic in colloid aqueous solution
The mass percent of sodium carboxymethylcellulose pyce is 1%, and the mass percent of sodium alginate is 4%, then is added 60 by 1.5% mass ratio
Purpose diatomite, stirs evenly, and what then instillation was stirred continuously contains 4%CaCl2And 2%MgSO4Mixed aqueous solution in, impregnate 2
The spheric granules of 2~3 mm of diameter is made in h, spare after being washed twice with sterile water.
(3) inoculation and fermentation
Granatanine made from (1) is extracted concentrated aqueous solution and is packed into fermentor, (the NH of mass ratio 0.2% is added4)2HPO4, then immobilized bacteria micelle access fermentor, inoculum concentration is the 12% of quality of fermentation broth ratio, and fermentation temperature is controlled 34
DEG C, be pumped into filtrated air from fermenter base, air mass flow be 0.5 times of fermentating liquid volume per hour, ferment 4.5 days.Filtering
Separation and fermentation liquid and immobilized bacteria micelle, strain micelle can be used continuously 20 times or more, and fermentation liquid is for extracting ellagic acid.
(4) it extracts
Fermentation liquid containing ellagic acid obtains the precipitating containing ellagic acid, the nothing of 5 times of volumes of the 1st addition by centrifuge separation
70 DEG C of 4 h of reflux extraction of water methanol, filtering；3 h of anhydrous methanol reflux extraction of 2nd 3 times of volume of addition into filter residue, mistake
Filter；Merge time filtrate, to being saturated, the concentrate of saturation is stood still for crystals revolving recycling methanol at room temperature, filters obtained solid knot
Crystalline substance is freeze-dried up to ellagic acid product after deionized water is washed.
Embodiment 3:
(1) prepared by granatanine extracting solution
Dry granatum is crushed to 40 mesh, using 75 DEG C of the ethanol solution refluxing extraction 3 times of 70% volume fraction,
1 addition ethanol solution is 15 times of Pomegranate Rind quality, extracts 2 h, is centrifugated filtrate and filter residue；2nd time to filter residue
Middle 8 times of addition ethanol solution, extract 1.5 h, centrifuge separation；3rd 6 times of ethanol solution of the addition into filter residue, extracts 1 h, from
Heart separation；Merge 3 filtrates, be distilled to recover ethyl alcohol, obtain granatanine and extract concentrated aqueous solution, can be directly used for fermentation life
It produces.Tannin content 1%~1.5% in concentrated aqueous solution is extracted after measured.
(2) culture of fermenting microbe is fixed together
By black-koji mould, Penicillium notatum and Escherichia coli respectively in liquid complete medium in 33 DEG C of 12 h of shaken cultivation
After (80r/min), 3 kinds of culture solutions are centrifuged 10 min respectively at 6000 r/min, spore and thallus suspension are obtained, with sterile water
Bacteria suspension is diluted, makes spore or thallus number up to 106~107Then a/mL is mixed 3 kinds of bacteria suspensions with 3: 2: 2 volume ratio
It after uniformly, then is mixed with the colloid aqueous solution of sodium carboxymethylcellulose and sodium alginate in 1: 1 ratio, carboxylic in colloid aqueous solution
The mass percent of sodium carboxymethylcellulose pyce is 1%, and the mass percent of sodium alginate is 4%, then 60 mesh are added by 2% mass ratio
Diatomite, stir evenly, then instill be stirred continuously containing 4%CaCl2And 2%MgSO4Mixed aqueous solution in, impregnate 2 h,
The spheric granules of 2~3 mm of diameter is made, it is spare after being washed twice with sterile water.
(3) inoculation and fermentation
Granatanine made from (1) is extracted concentrated aqueous solution and is packed into fermentor, (the NH of mass ratio 0.2% is added4)2HPO4, then immobilized bacteria micelle access fermentor, inoculum concentration is the 15% of quality of fermentation broth ratio, and fermentation temperature is controlled 35
DEG C, be pumped into filtrated air from fermenter base, air mass flow be 0.6 times of fermentating liquid volume per hour, ferment 4 days.Filtering point
From fermentation liquid and immobilized bacteria micelle, strain micelle can be used continuously 20 times or more, and fermentation liquid is for extracting ellagic acid.
(4) it extracts
Fermentation liquid containing ellagic acid obtains the precipitating containing ellagic acid, the nothing of 6 times of volumes of the 1st addition by centrifuge separation
70 DEG C of 4 h of reflux extraction of water methanol, filtering；3 h of anhydrous methanol reflux extraction of 2nd 2 times of volume of addition into filter residue, mistake
Filter；Merge time filtrate, to being saturated, the concentrate of saturation is stood still for crystals revolving recycling methanol at room temperature, filters obtained solid knot
Crystalline substance is freeze-dried up to ellagic acid product after deionized water is washed.
Claims (1)
1. a kind of method that granatanine liquid state fermentation prepares ellagic acid, which comprises the following steps:
(1) prepared by granatanine extracting solution
Dry granatum is crushed to 40 mesh, uses 60-80 DEG C of refluxing extraction of ethanol solution 3 of 61%~70% volume fraction
Secondary, the 1st addition ethanol solution is 10~15 times of Pomegranate Rind quality, extracts 2 h, is centrifugated filtrate and filter residue；2nd
It is secondary that 8~10 times of ethanol solution are added into filter residue, extract 1.5 h, centrifuge separation；The 3rd addition ethanol solution 6 into filter residue~
8 times, extract 1 h, centrifuge separation；Merge 3 filtrates, be distilled to recover ethyl alcohol, obtains granatanine and extract concentrated aqueous solution, it can
It is directly used in fermenting and producing, extracts tannin content 1%~1.5% in concentrated aqueous solution after measured；
(2) culture of fermenting microbe is fixed together
By black-koji mould, Penicillium notatum and Escherichia coli respectively in liquid complete medium in 33 DEG C of 12 h (80r/ of shaken cultivation
Min after), 3 kinds of culture solutions is centrifuged 12~10 min respectively at 5200~6000 r/min, obtain spore and thallus suspension, with
Sterile water dilutes bacteria suspension, makes spore or thallus number up to 106~107A/mL, then by 3 kinds of bacteria suspensions with 3: 2: 2 volume ratio
Example after mixing, then in 1: 1 ratio is mixed with the colloid aqueous solution of sodium carboxymethylcellulose and sodium alginate, and colloid is water-soluble
The mass percent of sodium carboxymethylcellulose is 1% in liquid, and the mass percent of sodium alginate is 4%, then presses 1%~2% quality
It than the diatomite that 60 mesh are added, stirs evenly, what then instillation was stirred continuously contains 4%CaCl2And 2%MgSO4Mixed aqueous solution
In, 2 h are impregnated, the spheric granules of 2~3 mm of diameter is made, it is spare after being washed twice with sterile water；
(3) inoculation and fermentation
Granatanine made from (1) is extracted concentrated aqueous solution and is packed into fermentor, (the NH of mass ratio 0.2% is added4)2HPO4,
Immobilized bacteria micelle is accessed fermentor again, inoculum concentration is the 10%~15% of quality of fermentation broth ratio, and fermentation temperature is controlled 33
~35 DEG C, be pumped into filtrated air from fermenter base, air mass flow be 0.4~0.6 times of fermentating liquid volume per hour, fermentation 4
~5 days, it is separated by filtration fermentation liquid and immobilized bacteria micelle, strain micelle can be used continuously 20 times or more, and fermentation liquid is for mentioning
Take ellagic acid；
(4) it extracts
Fermentation liquid containing ellagic acid obtains the precipitating containing ellagic acid by centrifuge separation, the anhydrous of 4~6 times of volumes is added the 1st time
70 DEG C of 4 h of reflux extraction of methanol, filtering；3 h of anhydrous methanol reflux extraction of 2nd 2~3 times of volume of addition into filter residue, mistake
Filter；Merge time filtrate, to being saturated, the concentrate of saturation is stood still for crystals revolving recycling methanol at room temperature, filters obtained solid knot
Crystalline substance is freeze-dried up to ellagic acid product after deionized water is washed.
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| KR20210114929A (en) * | 2018-11-05 | 2021-09-24 | 마블바이옴 인코포레이티드 | Microbial compositions comprising ellagitannins and methods of use |
| CN110357900A (en) * | 2019-08-02 | 2019-10-22 | 中国科学院新疆理化技术研究所 | A kind of intermediate processing obtaining ellagic acid from pomegranate rind extract |
| CN110668453A (en) * | 2019-11-12 | 2020-01-10 | 东海县博汇新材料科技有限公司 | Method for purifying superfine silica powder by using microorganism mixed strain |
| CN111544355B (en) * | 2020-06-04 | 2021-04-27 | 爱西美(珠海)生物技术有限公司 | Ellagic acid enzyme and preparation method thereof |
| CN112772789B (en) * | 2020-12-31 | 2023-05-09 | 内江师范学院 | Functional additive for pomegranate rind feed, preparation method and application |
| CN114107161B (en) * | 2021-11-15 | 2023-06-20 | 泸州老窖股份有限公司 | Method for producing ellagic acid by degrading pomegranate rind by mixed domesticated strain |
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| CN101481714A (en) * | 2008-01-11 | 2009-07-15 | 北京化工大学 | Method for preparing ellagic acid from pomegranate bark by enzyme process |
| CN102864184A (en) * | 2012-08-29 | 2013-01-09 | 太仓市茂通化建有限公司 | Method for producing citric acid by utilizing immobilized aspergillus niger |
| CN104341431A (en) * | 2014-09-30 | 2015-02-11 | 桂林三宝生物科技有限公司 | Method for extracting ellagic acid from pomegranate peel |
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2017
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101481714A (en) * | 2008-01-11 | 2009-07-15 | 北京化工大学 | Method for preparing ellagic acid from pomegranate bark by enzyme process |
| CN102864184A (en) * | 2012-08-29 | 2013-01-09 | 太仓市茂通化建有限公司 | Method for producing citric acid by utilizing immobilized aspergillus niger |
| CN104341431A (en) * | 2014-09-30 | 2015-02-11 | 桂林三宝生物科技有限公司 | Method for extracting ellagic acid from pomegranate peel |
Non-Patent Citations (2)
| Title |
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| 鞣花酸和三萜类物质在石榴不同器官中的分布;张立华;《食品科学》;20151231;第36卷(第6期);全文 * |
| 鞣花酸和三萜类物质在石榴不同器官中的分布;张立华;《食品科学》;20151231;第36卷(第6期);摘要 * |
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