AU2020102334A4 - Ellagic acid prepared from pomegranate rind by solid-state fermentation and its preparation method - Google Patents
Ellagic acid prepared from pomegranate rind by solid-state fermentation and its preparation method Download PDFInfo
- Publication number
- AU2020102334A4 AU2020102334A4 AU2020102334A AU2020102334A AU2020102334A4 AU 2020102334 A4 AU2020102334 A4 AU 2020102334A4 AU 2020102334 A AU2020102334 A AU 2020102334A AU 2020102334 A AU2020102334 A AU 2020102334A AU 2020102334 A4 AU2020102334 A4 AU 2020102334A4
- Authority
- AU
- Australia
- Prior art keywords
- fermentation
- pomegranate rind
- ellagic acid
- solid
- filtrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
- C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/66—Aspergillus
- C12R2001/685—Aspergillus niger
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Abstract
The invention involves a method of preparing ellagic acid from pomegranate rind by
solid-state fermentation, belonging to the technical field of cosmetics, medicine, and
health care products. In this invention, shallow plastic tanks are used to make
fermentation beds to facilitate loading and unloading and can be stacked in layers;
Aspergillus Niger and Escherichia. Coli are used for mixed fermentation to form
complementary effect so as to improve the transformation efficiency of ellagic acid;
after extraction with anhydrous methanol at decreasing dosage for three times, recover
methanol, wash residue with deionized water, and freeze-dry it to obtain the quality
product with high yield and purity of ellagic acid.
With simple operation and mild fermentation conditions, the process can greatly
reduce the consumption of chemical substances and cause less environmental
pollution, which is more suitable for large-scale production.
Description
PATENTS ACT 1990
The invention is described in the following statement:
The invention involves a kind of ellagic acid prepared from pomegranate rind by solid
state fermentation and its preparation method, belonging to the technical field of
cosmetics, medicine, and health care products.
Ellagic acid, with molecular formula C14H608, is a dimeric derivative of gallic acid and a
kind of polyphenol dilactone. It is a kind of natural polyphenol compound widely found
in various nuts and fruits, especially in pomegranate rind. However, it is less in free state
and more in condensed form (such as ellagitannin, glycoside, etc.). The pure ellagic acid
is a yellow needle crystal, which is slightly soluble in water and alcohol, and soluble in
hot methanol, alkali and pyridine.
In recent 20 years, it has been found that ellagic acid has strong ability to anti-lipid
peroxidation and capture free radicals. It has inhibitory effect on metabolic activity of
carcinogens to relieve their toxicity, and inhibitory effect on human immunodeficiency
virus. In addition, it has effects on gastrointestinal dynamics and urogenital dynamics, as
well as coagulation, hypotension and sedation. In view of these functions, people pay
more attention to ellagic acid.
Ellagic acid can be obtained by extraction, chemical synthesis and degradation of natural
products. The specific preparation methods are mainly as follows.
1. Extract ellagic acid from plants. There is less free ellagic acid (1%) in valonia,
raspberry, pomegranate and fern, and high purity ellagic acid can be prepared by this
method. But because of the high cost of raw materials and the low content of ellagic
acid, it has no practical significance in production.
2. Obtain ellagic acid through oxidation polymerization of gallic acid (or its ester).
In this method, the synthesized precursor-gallic acid (ester) produce ellagic acid
through oxidative polymerization in the presence of enzymes. The yield of ellagic
acid is 20% - 30%. However, the preparation process of gallic acid (ester) is
complicated, and the enzyme also needs to be refined and purified. Moreover, there
are unknown quinones in the synthetic products, with a ratio of 1:2.4 to ellagic acid,
which brings some difficulties to the separation and purification of products. At
present, this method is rarely used in the production of ellagic acid.
3. Prepare ellagic acid by acid-hydrolysis method (Patent No.200510002081.X). This
process has higher yield and purity, but it needs to consume strong acid with high
concentration and to be completed under the condition of long-time heating, which
requires high anti corrosion property of equipment and causes heavy environmental
pollution.
4. Non-chemical preparation method of ellagic acid from pomegranate rind disclosed in
this invention. One is enzymatic method (Patent No.200810056007.X), and it can be
divided into two steps- enzymes production through liquid fermentation and enzyme
catalyzed synthesis. This method is cumbersome, and the enzyme-catalyzed synthesis conditions are strict and difficult to control. Another disclosed method is
Aspergillus Niger solid-state fermentation (Patent No.201010178430.4). In this
method, pomegranate rind powder is placed in a fermentation tank, and wet
pomegranate rind with initial wetting water solution accounting for 47%-60% of the
total weight, wherein, there is 0.1%0 NaCl, 0.1 % MgSO4and0.5%NH4NO3inthe
wetting solution, and NH4NO3 is used as N source with a pH value of 5-7. Sterilize
the solution at 115°C for 30 min, cool it and then inoculate Aspergillus Niger 3.316
spore suspension. The ratio of pomegranate rind to Aspergillus Niger is to add 1mL
Aspergillus Niger spore suspension with a concentration of 1x101 /mL to 5 g
pomegranate rind. Uniformly mix and stand for culture, then ferment at 30°C for 5-7
days to obtain a fermentation product containing ellagic acid. The method uses
fermentation tank for solid-state fermentation, which is troublesome in charging and
discharging, and ferment unevenly in upper and lower parts. Furthermore, the
method only uses a single strain for fermentation and has a low efficiency, which has
certain limitations on large-scale industrial production.
The invention aims to provide a more efficient method of preparing ellagic acid which
takes pomegranate rind as substrate and adopts solid-state fermentation. Taking
pomegranate rind powder as fermentation substrate, ellagic acid is produced by solid
state fermentation of Aspergillus Niger and Escherichia. Coli and high purity ellagic acid
can be obtained by methanol extraction. The method is simple, and the obtained ellagic
acid are with high purity and yield.
The invention takes Aspergillus Niger and Escherichia. Coli as fermentation strains and
pomegranate rind powder as substrate for solid-state fermentation. In the fermentation
process, both Aspergillus Niger and Escherichia. Coli synthesize tannase and secrete it to
extracellular. Tannase can catalyses the degradation of ellagitannin in pomegranate rind
to produce ellagic acid. Then extract and purify the fermentation product to obtain high
purity ellagic acid. The method specifically comprises following steps.
(1) Preparation of fermentation bacterial suspension
First use purified water to prepare nutrient solution, which comprises 0.5%-1% of
(NH4)2HP03, 0.1%-0.2% of MgCl2 and 0.1%-0.2% of Na2SO4. 10 ml of Escherichia.
Coli suspension and 10 ml of Aspergillus Niger spore suspension are added to each 1 L
nutrient solution to make the number of Escherichia. Coli more than 1 x 107 / mL and
Aspergillus Niger spores more than 1 x 108 / mL respectively.
(2) Preparation of pomegranate rind fermentation beds
Evenly spray fermentation bacteria suspension prepared by (1) on the pomegranate rind
powder, wherein, the amount of bacteria suspension accounts for 40% - 45% of the
pomegranate rind powder weight. Then put the inoculated pomegranate rind powder into
a square plastic tank with height of 10-15 cm, width of 30 cm, and length of 40 cm.
Besides, the length and width can also be adjusted according to needs. Load materials to
the upper edge of the plastic tanks to make pomegranate rind fermentation beds and put
them on the shelf in the fermentation room.
(3) Solid-state fermentation of pomegranate rind
Control the temperature of fermentation room in the range of 31-35C and keep the
humidity between 85% - 9 0 %. After standing for 4-5 days, the fermentation is stopped, and the fermentation products containing ellagic acid are obtained by drying in the sun or stoving.
(4) Extraction
Comminute the dried fermentation products for three times. For the first time, add 8-9
times volume of anhydrous methanol to conduct reflux extraction at 70 °C for 3 hours,
and then filtrate; for the second time, add 6-7 times volume of anhydrous methanol into
the filter residue for reflux extraction for 2 hours and then filtrate again; for the third
time, add 4-5 times of anhydrous methanol into the obtained filter residue for reflux
extraction for 1 hour and then filtrate again. Combine the filtrate of 3 times, recover
methanol by rotary evaporation, and wash the obtained solid with deionized water and
then freeze-dry it to obtain ellagic acid.
The essence of the invention is that taking pomegranate rind as solid-state fermentation
substrate, Aspergillus Niger and Escherichia. Coli can produce tannase with high
specificity to hydrolyse the ester bonds of ellagitannin to produce gallic acid.
After extraction and purification, high purity ellagic acid can be obtained.
The advantages of the invention are that shallow plastic tanks are used to make
fermentation beds to facilitate loading and unloading and can be stacked in layers;
Aspergillus Niger and Escherichia. Coli are used for mixed fermentation to form
complementary effect so as to improve the transformation efficiency of ellagic acid and
obtain the quality product with high yield and purity of ellagic acid. With simple
operation and mild fermentation conditions, the process can greatly reduce the
consumption of chemical substances and cause less environmental pollution, which is
more suitable for large-scale production. In China, there is a large planting area of pomegranate and rich pomegranate peel resources. Except a small part of pomegranate is used as Chinese medicinal material, most of them are abandoned, resulting in great waste.
The invention takes pomegranate rind as raw material, has low production cost and
improves product added value.
The invention is further described in combination with the embodiment below:
Embodiment 1
(1) Preparation of fermentation bacterial suspension
First use purified water to prepare nutrient solution, which comprises 0.5% of
(NH4)2HP03, 0.1% of MgCl2 and 0.1% of Na2SO4. 10 ml of Escherichia. Coli suspension
and 10 ml of Aspergillus Niger spore suspension are added to each 1 L nutrient solution
to make the number of Escherichia. Coli more than 1 x 107 / mL and Aspergillus Niger
spores more than 1 x 108 / mL respectively.
(2) Preparation of pomegranate rind fermentation beds
Evenly spray fermentation bacteria suspension prepared by (1) on the pomegranate rind
powder, wherein, the amount of bacteria suspension accounts for 40% of the
pomegranate rind powder weight. Then put the inoculated pomegranate rind powder into
a square plastic tank with height of 10 cm, width of 30 cm, and length of 40 cm. Besides,
the length and width can also be adjusted according to needs. Load materials to the upper
edge of the plastic tanks to make pomegranate rind fermentation beds and put them on the
shelf in the fermentation room.
(3) Solid-state fermentation of pomegranate rind
Control the temperature of fermentation room at 31°C and keep the humidity at 85%.
After standing for 5 days, the fermentation is stopped, and the fermentation products
containing ellagic acid are obtained by drying in the sun or stoving.
(4) Extraction
Comminute the dried fermentation products for three times. For the first time, add 8 times
volume of anhydrous methanol to conduct reflux extraction at 70 °C for 3 hours, and
then filtrate; for the second time, add 7 times volume of anhydrous methanol into the
filter residue for reflux extraction for 2 hours and then filtrate again; for the third time,
add 5 times of anhydrous methanol into the obtained filter residue for reflux extraction
for 1 hour and then filtrate again. Combine the filtrate of 3 times, recover methanol by
rotary evaporation, and wash the obtained solid with deionized water and then freeze-dry
it to obtain ellagic acid.
Embodiment 2
(1)Preparation of fermentation bacterial suspension
First use purified water to prepare nutrient solution, which comprises 1% of (NH4)2HP03,
0.1% of MgCl2 and 0.2% of Na2SO4. 10 ml of Escherichia. Coli suspension and 10 ml of
Aspergillus Niger spore suspension are added to each 1 L nutrient solution to make the
number of Escherichia. Coli more than 1 x 107 / mL and Aspergillus Niger spores more
than 1 x 108 / mL respectively.
(2)Preparation of pomegranate rind fermentation beds
Evenly spray fermentation bacteria suspension prepared by (1) on the pomegranate rind
powder, wherein, the amount of bacteria suspension accounts for 45% of the pomegranate rind powder weight. Then put the inoculated pomegranate rind powder into a square plastic tank with height of 15 cm, width of 30 cm, and length of 40 cm. Besides, the length and width can also be adjusted according to needs. Load materials to the upper edge of the plastic tanks to make pomegranate rind fermentation beds and put them on the shelf in the fermentation room.
(3)Solid-state fermentation of pomegranate rind
Control the temperature of fermentation room at 35C and keep the humidity at 90%.
After standing for 4 days, the fermentation is stopped, and the fermentation products
containing ellagic acid are obtained by drying in the sun or stoving.
(4)Extraction
Comminute the dried fermentation products for three times. For the first time, add 9 times
volume of anhydrous methanol to conduct reflux extraction at 70 °C for 3 hours, and
then filtrate; for the second time, add 6 times volume of anhydrous methanol into the
filter residue for reflux extraction for 2 hours and then filtrate again; for the third time,
add 4 times of anhydrous methanol into the obtained filter residue for reflux extraction
for 1 hour and then filtrate again. Combine the filtrate of 3 times, recover methanol by
rotary evaporation, and wash the obtained solid with deionized water and then freeze-dry
it to obtain ellagic acid.
Embodiment 3
(1)Preparation of fermentation bacterial suspension
First use purified water to prepare nutrient solution, which comprises 0.5% of
(NH4)2HP3, 0.2% of MgCl2 and 0.2% of Na2SO4. 10 ml of Escherichia. Coli suspension
and 10 ml of Aspergillus Niger spore suspension are added to each 1 L nutrient solution to make the number of Escherichia. Coli more than 1 x 107 / mL and Aspergillus Niger spores more than 1 x 108 / mL respectively.
(2)Preparation of pomegranate rind fermentation beds
Evenly spray fermentation bacteria suspension prepared by (1) on the pomegranate rind
powder, wherein, the amount of bacteria suspension accounts for 43% of the
pomegranate rind powder weight. Then put the inoculated pomegranate rind powder into
a square plastic tank with height of 13 cm, width of 30 cm, and length of 40 cm. Besides,
the length and width can also be adjusted according to needs. Load materials to the upper
edge of the plastic tanks to make pomegranate rind fermentation beds and put them on the
shelf in the fermentation room.
(3)Solid-state fermentation of pomegranate rind
Control the temperature of fermentation room at 33C and keep the humidity at 88%.
After standing for 5 days, the fermentation is stopped, and the fermentation products
containing ellagic acid are obtained by drying in the sun or stoving.
(4)Extraction
Comminute the dried fermentation products for three times. For the first time, add 8.5
times volume of anhydrous methanol to conduct reflux extraction at 70 °C for 3 hours,
and then filtrate; for the second time, add 6.5 times volume of anhydrous methanol into
the filter residue for reflux extraction for 2 hours and then filtrate again; for the third
time, add 4-4.5 times of anhydrous methanol into the obtained filter residue for reflux
extraction for 1 hour and then filtrate again. Combine the filtrate of 3 times, recover
methanol by rotary evaporation, and wash the obtained solid with deionized water and
then freeze-dry it to obtain ellagic acid.
Claims (4)
1. A method of preparing ellagic acid from pomegranate rind by solid-state
fermentation, characterized by comprising following steps:
(1) Preparation of fermentation bacterial suspension
First use purified water to prepare nutrient solution, which comprises 0.5%-l % of
(NH4)2HP03, 0.1%-0.2% of MgCl2 and 0.1%-0.2% of Na2SO4. 10 ml of Escherichia.
Coli suspension and 10 ml of Aspergillus Niger spore suspension are added to each 1 L
nutrient solution to make the number of Escherichia. Coli more than 1 x 107 / mL and
Aspergillus Niger spores more than 1 x 108 / mL respectively.
(2) Preparation of pomegranate rind fermentation beds
Evenly spray fermentation bacteria suspension prepared by (1) on the pomegranate rind
powder, wherein, the amount of bacteria suspension accounts for 40% - 45% of the
pomegranate rind powder weight. Then put the inoculated pomegranate rind powder into
square plastic tanks to make pomegranate rind fermentation beds and stack them on the
shelf in the fermentation room.
(3) Solid-state fermentation of pomegranate rind
Control the temperature of fermentation room in the range of 31-35C and keep the
humidity between 85% - 9 0 %. After standing for 4-5 days, the fermentation is stopped,
and the fermentation products containing ellagic acid are obtained by drying in the sun or
stoving.
(4) Extraction
Comminute the dried fermentation products for three times. For the first time, add 8-9
times volume of anhydrous methanol to conduct reflux extraction at 70 °C for 3 hours, and then filtrate; for the second time, add 6-7 times volume of anhydrous methanol into the filter residue for reflux extraction for 2 hours and then filtrate again; for the third time, add 4-5 times of anhydrous methanol into the obtained filter residue for reflux extraction for 1 hour and then filtrate again. Combine the filtrate of 3 times, recover methanol by rotary evaporation, and wash the obtained solid with deionized water and then freeze-dry it to obtain ellagic acid.
2. The method of preparing ellagic acid from pomegranate rind by solid-state
fermentation, according to claim 1, characterized in that each square plastic tank is in
height of 10-15 cm, width of 30 cm, and length of 40 cm. Besides, the length and width
can also be adjusted according to needs. Materials are needed to be loaded to the upper
edge of the plastic tanks.
3. A method of preparing the ellagic acid from pomegranate rind by solid-state
fermentation according to claim 1 and claim 2, characterized by including following
steps:
(1)Preparation of fermentation bacterial suspension
First use purified water to prepare nutrient solution, which comprises 0.5%-l0% of
(NH4)2HP03, 0.1%-0.2% of MgCl2 and 0.1%-0.2% of Na2SO4. 10 ml of Escherichia.
Coli suspension and 10 ml of Aspergillus Niger spore suspension are added to each 1 L
nutrient solution to make the number of Escherichia. Coli more than 1 x 107 / mL and
Aspergillus Niger spores more than 1 x 108 / mL respectively.
(2)Preparation of pomegranate rind fermentation beds
Evenly spray fermentation bacteria suspension prepared by (1) on the pomegranate rind
powder, wherein, the amount of bacteria suspension accounts for 40% - 45% of the pomegranate rind powder weight. Then put the inoculated pomegranate rind powder into square plastic tanks to make pomegranate rind fermentation beds and stack them on the shelf in the fermentation room.
(3)Solid-state fermentation of pomegranate rind
Control the temperature of fermentation room in the range of 31-35C and keep the
humidity between 85% -90%. After standing for 4-5 days, the fermentation is stopped,
and the fermentation products containing ellagic acid are obtained by drying in the sun or
stoving.
(4)Extraction
Comminute the dried fermentation products for three times. For the first time, add 8-9
times volume of anhydrous methanol to conduct reflux extraction at 70 °C for 3 hours,
and then filtrate; for the second time, add 6-7 times volume of anhydrous methanol into
the filter residue for reflux extraction for 2 hours and then filtrate again; for the third
time, add 4-5 times of anhydrous methanol into the obtained filter residue for reflux
extraction for 1 hour and then filtrate again. Combine the filtrate of 3 times, recover
methanol by rotary evaporation, and wash the obtained solid with deionized water and
then freeze-dry it to obtain ellagic acid.
4. The method of preparing the ellagic acid from pomegranate rind by solid-state
fermentation according to claim 3, characterized in that each square plastic tank is in
height of 10-15 cm, width of 30 cm, and length of 40 cm. Besides, the length and width
can also be adjusted according to needs. Materials are needed to be loaded to the upper
edge of the plastic tanks.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2020102334A AU2020102334A4 (en) | 2020-09-18 | 2020-09-18 | Ellagic acid prepared from pomegranate rind by solid-state fermentation and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2020102334A AU2020102334A4 (en) | 2020-09-18 | 2020-09-18 | Ellagic acid prepared from pomegranate rind by solid-state fermentation and its preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2020102334A4 true AU2020102334A4 (en) | 2020-10-29 |
Family
ID=72926615
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2020102334A Ceased AU2020102334A4 (en) | 2020-09-18 | 2020-09-18 | Ellagic acid prepared from pomegranate rind by solid-state fermentation and its preparation method |
Country Status (1)
| Country | Link |
|---|---|
| AU (1) | AU2020102334A4 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107403A (en) * | 2021-11-29 | 2022-03-01 | 郑州轻工业大学 | Method for co-production of ellagic acid and biological feed by fermenting pomegranate rind with microbial community |
| CN114561281A (en) * | 2022-02-21 | 2022-05-31 | 广州市博之越精细化工有限公司 | Synthesis process and synthesis device of micromolecular ellagic acid |
-
2020
- 2020-09-18 AU AU2020102334A patent/AU2020102334A4/en not_active Ceased
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107403A (en) * | 2021-11-29 | 2022-03-01 | 郑州轻工业大学 | Method for co-production of ellagic acid and biological feed by fermenting pomegranate rind with microbial community |
| CN114107403B (en) * | 2021-11-29 | 2023-08-01 | 郑州轻工业大学 | Method for co-producing ellagic acid and biological feed by fermenting pericarpium Granati with microbial community |
| CN114561281A (en) * | 2022-02-21 | 2022-05-31 | 广州市博之越精细化工有限公司 | Synthesis process and synthesis device of micromolecular ellagic acid |
| CN114561281B (en) * | 2022-02-21 | 2022-10-25 | 广州市博之越精细化工有限公司 | Synthesis process and synthesis device of micromolecular ellagic acid |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2020102334A4 (en) | Ellagic acid prepared from pomegranate rind by solid-state fermentation and its preparation method | |
| CN107418995B (en) | A kind of ellagic acid and preparation method thereof of granatanine liquid state fermentation preparation | |
| CN102250981B (en) | Method for preparing ellagic acid by solid fermentation with granatum as raw material | |
| CN101481714A (en) | Method for preparing ellagic acid from pomegranate bark by enzyme process | |
| WO2014012418A1 (en) | Method for preparing short-chain fatty acid having high propanoic acid content by continuous fermentation | |
| CN114107403B (en) | Method for co-producing ellagic acid and biological feed by fermenting pericarpium Granati with microbial community | |
| CN103805651A (en) | Production method for pullulan based on cell metabolism regulation strategies | |
| CN104805143A (en) | Method for preparing low molecular weight gamma-polyglutamic acid | |
| KR101740968B1 (en) | Method for production of GABA with lactic acid bacteria through L-glutamic acid feeding | |
| CN103864954A (en) | Method for extracting peanut meal polysaccharide | |
| CN100584953C (en) | A kind of is the method that raw material is produced γ-An Jidingsuan with rice | |
| CN105219804A (en) | A kind of molasses fermented method preparing microbial flocculant | |
| CN104800110B (en) | A kind of method that microbial fermentation prepares total saponin of sapindusmukerossi | |
| CN102703329B (en) | Strain for efficiently transforming saponin in turmeric to produce diosgenin, and application thereof | |
| CN104031109A (en) | Method for purifying tea saponin by microbial fermentation | |
| CN104531810A (en) | Method for preparing maltonic acid through efficient microbial conversion | |
| CN105211630A (en) | Humic acid biological feed additive and production method | |
| CN105331643A (en) | Method for preparing L-lactic acid through fermentation of sophora flower bud decoction dregs | |
| KR20200137409A (en) | Iodine GABA Salt and Preparing Method Thereof | |
| CN102605009B (en) | Method for improving strength and concentration of butane diacid generated from anaerobic fermentation | |
| CN107400686A (en) | Ellagic acid prepared by a kind of solid state fermentation granatum and preparation method thereof | |
| KR101750619B1 (en) | Preparation method of osmotic enzyme fermentation product using momordica charantia and houttuynia cordata, the fermentation product prepared thereby and cosmetic, food or pharmaceutical composition comprising the same | |
| CN104726515A (en) | Method for extracting bacterial exopolysaccharide rich in fucose | |
| CN104892554B (en) | The preparation method of a kind of gibberic acid GA3 | |
| KR101477229B1 (en) | A Method for Preparing Bacterial Cellulose Using Steamed Dregs of Citrus Fruits |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGI | Letters patent sealed or granted (innovation patent) | ||
| MK22 | Patent ceased section 143a(d), or expired - non payment of renewal fee or expiry |