CN107418899A - One plant of beauveria bassiana BbL25 bacterial strain for preventing and treating red fire ant and its application - Google Patents
One plant of beauveria bassiana BbL25 bacterial strain for preventing and treating red fire ant and its application Download PDFInfo
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Abstract
The invention discloses the beauveria bassiana BbL25 bacterial strains of one plant of preventing and treating red fire ant.The bacterial strain was preserved in China typical culture collection center on 2 16th, 2017, and culture presevation number is CCTCC NO：M2017045.Beauveria bassiana BbL25 bacterial strains of the present invention highly cause a disease to red fire ant, the 14th day half lethal concentration to red fire ant after it is inoculated with（LC50）For 8.15 × 105Conidium/mL, and the bacterial strain has preferable field control effect to red fire ant, 1 × 107/ mL spore suspension reaches 82.52% in 14 days after administration to the prevention effect of red fire ant, suitable with the effect of the sulfluramid bait formulation of chemical insecticide 0.25%.The beauveria bassiana BbL25 bacterial strains can be used as a kind of living body biological insecticide, environmentally safe, non-toxic to people and animals, produced suitable for organic farm products, have larger application prospect.
Description
Technical field
The invention belongs to technical field of biological control.More particularly, to the beauveria bassiana of one plant of preventing and treating red fire ant
BbL25 bacterial strains and its application.
Background technology
Red fire ant Solenopsis invicta Buren belong to Hymenoptera Hymenoptera Formicidaes Formicidae and cut leaf
Formicinae Myrmicinae Solenopsis Solenopsis, red fire ant are a kind of allusion quotations as generally acknowledged harmfulness Invasive Alien Species
The agriculture and forestry insect of type, causes to seriously endanger to invaded area of institute.In China, red fire ant invaded Taiwan, Guangdong, Hong Kong,
11 provinces and regions such as Macao, Fujian, Guangxi, Hunan, Hainan, Sichuan, Jiangxi, Yunnan.Red fire ant possesses powerful destructive power and attacked
Hitting property, the seed of feeding plant, invertebrate is preyed on, venom bites humans and wild animal are discharged by anal spine, sting food electricity
Sub- electric equipment insulation damage layer Civil Infrastructure Systems etc..To human health, bio-diversity, agriculture and forestry production and infrastructure
Safety causes serious threat.
The preventing and treating of red fire ant mainly has the methods of physical control, chemical prevention, biological control, wherein using chemical insecticide
It is main method.But long-term use of chemical prevention can cause red fire ant to develop immunity to drugs problem, needed during preventing and treating constantly
Chemical agent usage amount is increased, so that cost accounting improves constantly.Other chemical pesticide it is excessive using easily to soil and its
The non-target organism of surrounding damages and polluted, and influences Environmental security and bio-diversity.The security of chemical pesticide control
The key factor of limitation Solenopsis invicta is turned into, and has carried out and become using biological control method preventing and treating red fire ant as one kind
Gesture.
The recent biological control to red fire ant both at home and abroad has carried out numerous studies, studies using natural enemy insect, predation
Property mite class, parasitic nematode and pathogenic microorganism are prevented and treated.Fungus insecticide has host range wide, environmentally safe, anti-
The advantages that insect does not develop immunity to drugs is controlled, is the promising approach for preventing and treating red fire ant.There are a variety of entomogenous fungis to infect red fire ant, such as
Mould Isaria fumosorosea of rose dark brown rod spore, yellowish green green muscardine fungus Metarhizium flavoviride, Metarhizium anisopliae
Metarhizium anisoplia and beauveria bassiana Beauveria bassiana, Paecilomyces lilacinus Paecilomyces
Lilacinus etc., and some existing related invention patents disclose：Number of patent application 201610656107.0 discloses one plant of anti-purple
(deposit number is CCTCC NO to the high virulence Metarhizium anisopliae mutagenic strain MaUV 1 of outside line：M 2016250) and its application,
The insects such as bacterial strain confrontation property of medicine diamondback moth, red fire ant infect insecticidal effect with stronger, and anti-uv-ray is strong；Specially
Sharp application number 201510103305.X discloses a kind of microbial insecticide composition for preventing and treating red fire ant, muscardine and thiacloprid
Compounding has significant synergistic effect, and prevention effect is very good, has the advantages of efficient, quick to red fire ant；Number of patent application
201310721119.3 disclosing a kind of fumosorosea and AVM oil-suspending agent and its preparation method and application, this is outstanding
Floating agent is mainly used in the preventing and treating of the insects such as diamondback moth and red fire ant；Number of patent application 201120139109.5 discloses one kind and trapped
Red fire ant infects the device of entomogenous fungi, and preventing and treating red fire ant effect is fine, the conidium of its device implants oil meter face adhesion
For the conidium of Java Isaria bacterium P028 bacterial strains；Number of patent application 200810029158.6 discloses a kind of Paecilomyces javanicus
Bacterial strain and its application, Paecilomyces javanicus have very strong infect to insects such as aleyrodid, red fire ant, prodenia litura, aphid, diamondback moths
Insecticidal effect；Number of patent application 200810029745.5 discloses a kind of compounded pesticides of Paecilomyces javanicus and nimbin, right
The insects such as red fire ant, prodenia litura, aphid, aleyrodid and diamondback moth have good prevention effect.
More also caused a disease on red fire ant entomogenous fungi research report, Lv Lihua etc. (2011) by determine 6 plants not
Insect pathogenic fungus with source is to the pathogenecity of red fire ant, it was found that beauveria bassiana Bb02, Bb04 bacterial strain tool are stronger
Pathogenicity and lethal speed, it is the strain excellent of red fire ant microbial control；Liu Xiaoyan etc. (2010) to pick up from Guangzhou Fanyu District,
The red fire ant polypide on the ground such as Nansha District, Huizhou and Shenzhen carries out the separation and identification of pathogen, and measure finds PL pairs of isolate
Red fire ant has certain pathogenic effects；Liu Xiaoyan etc. (2014) is used by setting 17,21,25,29 and 33 DEG C of 5 temperature levels
Spray-on process, it is 1 × 10 by concentration5With 1 × 108Individual mL-1Beauveria bassiana spore suspension infect red fire ant worker ant respectively, with test
Pathogenicity of the muscardine bacterial strain to red fire ant worker ant under different temperatures；(2009) are waited to screen indoors after Yang Jia quick to red fire ant
4 plants of Strain of Beauveria bassiana of sense, as a result find that this 4 plants of bacterium have certain potentiality in the biological control of red fire ant；Wang Lei
Have studied virulence of the various dose Metarhizium anisopliae M09 to red fire ant Deng (2014), the results showed that the death rate of red fire ant with
The dosage of Metarhizium anisopliae is proportionate；Xu Qiai etc. (2013) has screened the excellent biological and ecological methods to prevent plant disease, pests, and erosion of one plant of prevention and control red fire ant harm
Bacterial strain-yellowish green Metarhizium Strains SM076.
But the effect of above-mentioned pathogenic entomogenous fungi prevention and control red fire ant is not satisfactory, and study all simply bars indoors
Carried out under part, because indoor measurement condition is different from practical application, the pathogenicity of the envirment factor dialogue deadlock such as humiture has very
Big influence (Liang Zongqi and Ye Yuchang, 1994；Pan Zhiping, 2008), thus these entomogenous fungis in field conditions to flourishing
The prevention effect of ant is unofficial.
The content of the invention
The technical problems to be solved by the invention are to overcome the defects of pathogenic entomogenous fungi of red fire ant is present in the prior art
And deficiency, there is provided the beauveria bassiana BbL25 bacterial strains of one plant of preventing and treating red fire ant.The beauveria bassiana BbL25 bacterial strains are to flourishing
Ant is highly caused a disease, and field control effect is preferable, can be used as a kind of living body biological insecticide, environmentally safe, nontoxic to people and animals
Property, produced suitable for organic farm products, there is larger application prospect.
It is an object of the invention to provide the beauveria bassiana BbL25 bacterial strains of one plant of preventing and treating red fire ant.
It is a further object of the present invention to provide the application of above-mentioned beauveria bassiana BbL25 bacterial strains.
The above-mentioned purpose of the present invention is to give realization by the following technical programs：
One plant preventing and treating red fire ant beauveria bassiana (Beauveria bassiana) BbL25 bacterial strains, the bacterial strain in
China typical culture collection center (CCTCC) is preserved within 2 16th, 2017, culture presevation numbering is CCTCC NO：
M2017045, Classification And Nomenclature number are Beauveria bassiana BbL25；Preservation address is Wuhan, China Wuhan University.
The bacterial strain is washed one's hair by Chifeng Balin Right Banner hila and acquisition is isolated and purified in the soil to fall, living by biology
Property measure, biological characteristic research strain idenfication, it is the bacterium to red fire ant efficient entomogenous fungi bacterial strain to specify the bacterial strain
Strain classification position belongs to Deuteromycotina (Deuteromycotina), Hyphomycetes (Hyphomycetes), Moniliales
(Moniliales), Moniliaceae (Moniliaceae), Beauveria (Beauveria).
Morphological feature of the bacterial strain on potato dextrose medium is as follows：
Bacterium colony initial stage is white, surface villiform；By the 12nd day, bacterium colony was circular for bacterium colony culture, center and the micro- protuberance in edge,
Middle part mostly general plan micro-pits, surface is white, villiform, and the back side is faint yellow, a diameter of (68.16 ± 1.96) mm,
It is changed into powdered in the middle part of later stage bacterium colony.Under an optical microscope, mycelia have every, have branch, transparent, width is (1.08 ± 0.14)
μm；Conidiogenous cell is verticillate or Dan Sheng, and branch, conidiophore ultimate swelling, conidium do not grow densely conidiophore
On conidiophore；Conidium is spherical or subsphaeroidal, unit cell, colourless, diameter (2.05 ± 0.13) μm.
Further, the ITS1-5.8S-ITS4rDNA sequences of the beauveria bassiana BbL25 bacterial strains such as SEQ ID NO：1
It is shown.
Further, the 'beta '-tubulin gene order of the beauveria bassiana BbL25 bacterial strains such as SEQ ID NO：2 institutes
Show.
The 14th day semilethal to red fire ant is dense after the beauveria bassiana BbL25 bacterial strains of the present invention are inoculated with red fire ant indoors
Spend (LC50) it is 8.15 × 105Conidium/mL and the bacterial strain have preferable field control effect to red fire ant, and 1 × 107/ mL's
Spore suspension reaches 82.52% in 14 days after administration to the prevention effect of red fire ant, with the sulfluramid of chemical insecticide 0.25%
The effect of bait formulation is suitable.
The 2nd~3 day after the conidial suspension dipping red fire ant of the beauveria bassiana BbL25 bacterial strains, red fire ant goes out
Existing slow in action, flagging phenomenon, then occur dead.Under an optical microscope, can be observed white hypha first from
The podarthrum of red fire ant corpse, mouthpart, stretch out at body segment external, elapse over time, a large amount of mitogenetic spores of white are produced on mycelia
Son.
Therefore, application of the beauveria bassiana BbL25 bacterial strains in red fire ant is prevented and treated and red fire ant medicine is prevented and treated preparing
Application in agent is also in the scope of the present invention.
Preferably, the method for the application is：The conidium of beauveria bassiana BbL25 bacterial strains is harvested, with 0.01~
0.03% (preferably 0.02%) Tween 80 solution is configured to concentration as 5 × 106~1 × 107/ mL (preferably 1 × 107/ mL) spore
Suspension, spore suspension is sprayed on red fire ant using spray-on process and carries out Solenopsis invicta.It can specifically gather in red fire ant
It is sprayed on when combining in formicary surface with red fire ant so as to be prevented and treated.
Meanwhile the present invention also provides a kind of biological agent for preventing and treating red fire ant, includes above-mentioned ball in the biological agent
Beauveria bassiana BbL25 bacterial strains.
Preferably, 5 × 10 are included in the biological agent6~1 × 107Spore/mL beauveria bassiana BbL25 bacterial strains
Spore suspension.
It is highly preferred that include 1 × 10 in the biological agent7Spore/mL beauveria bassiana BbL25 bacterial strain spores hang
Supernatant liquid.
Meanwhile application of the above-mentioned biological agent in red fire ant is prevented and treated is also in the scope of the present invention.
Compared with prior art, the invention has the advantages that：
(1) beauveria bassiana BbL25 bacterial strains of the present invention to preventing and treating red fire ant it is efficient, after dispenser 14 days to red fire ant
Half lethal concentration (LC50) it is 8.15 × 105/mL。
(2) beauveria bassiana BbL25 bacterial strains of the present invention have preferable field control effect to red fire ant, and 1 × 107/
ML spore suspension reaches 82.52% in 14 days after administration to the prevention effect of red fire ant, with the fluorine of chemical insecticide 0.25%
The effect of worm amine bait formulation is suitable.
(3) beauveria bassiana BbL25 bacterial strains of the present invention are a kind of insect pathogenic fungus, can be used as biological living agriculture
Medicine, have it is environmentally safe, to the avirulent feature of people and animals, suitable for organic farm products production requirement, have it is larger should
Use prospect.
Brief description of the drawings
Fig. 1 be beauveria bassiana BbL25 bacterial strains on potato dextrose medium, 25 DEG C, culture 12 days when bacterial strain
Colonial morphology；A is front, and B is reverse side.
Fig. 2 is beauveria bassiana BbL25 bacterial strains conidial fructification and conidium micrograph；A is conidial fructification, and B is mitogenetic
Spore.
Fig. 3 is morphological feature when beauveria bassiana BbL25 bacterial strains infect red fire ant.
Fig. 4 is the ITS1-5.8S-ITS4rDNA genes and 'beta '-tubulin gene specific of beauveria bassiana BbL25 bacterial strains
Property amplification electrophoretogram.
Fig. 5 is the rDNA-ITS gene orders branch development tree of beauveria bassiana BbL25 bacterial strains.
Fig. 6 is the 'beta '-tubulin gene order branch development tree of beauveria bassiana BbL25 bacterial strains.
Embodiment
The present invention is made with reference to Figure of description and specific embodiment and further being elaborated, the embodiment
It is served only for explaining the present invention, is not intended to limit the scope of the present invention.Test method used in following embodiments is such as without spy
Different explanation, is conventional method；Used material, reagent etc., unless otherwise specified, for the reagent commercially obtained
And material.
The separation and identification of the beauveria bassiana BbL25 bacterial strains of embodiment 1
1st, strain isolation
The bacterial strain is washed one's hair by Chifeng Balin Right Banner hila and acquisition is isolated and purified in the soil to fall.
2nd, the Morphological Identification of bacterial strain
The bacterial strain of acquisition is cultivated on potato dextrose medium, its morphological feature is：Bacterium colony initial stage is white,
Surface villiform；By the 12nd day, bacterium colony was circular for bacterium colony culture, center and the micro- protuberance in edge, middle part mostly general plan micro-pits,
Surface is white, villiform, and the back side is faint yellow, a diameter of (68.16 ± 1.96) mm, is changed into powdered in the middle part of later stage bacterium colony
(as shown in Figure 1).Under an optical microscope, mycelia have every, have branch, transparent, width is (1.08 ± 0.14) μm；Conidiogenous cell
Verticillate or Dan Sheng, branch, conidiophore ultimate swelling, conidium are not grown in conidiophore densely to conidiophore
On；Conidium is spherical or subsphaeroidal, monospore, colourless, diameter (2.05 ± 0.13) μm (as shown in Figure 2).
3rd, the Molecular Identification of bacterial strain
The bacterial strain of acquisition is subjected to further Molecular Identification.The ITS1-5.8S-ITS4rDNA gene specifics of the bacterial strain
The electrophoretogram of property amplified matter and 'beta '-tubulin gene-specific amplification thing is as shown in Figure 4；Its corresponding ITS1-5.8S-ITS4
RDNA gene orders and 'beta '-tubulin gene order are successively such as SEQ ID NO：Shown in 1~2.
ITS1-5.8S-ITS4rDNA gene orders are compared in ncbi database, comparing result shows, the strain sequence
It is highly similar to multiple beauveria bassianas (Beauveria bassiana) strain sequence.By the bacterial strain and several beauveria bassianas
Bacterial strain, and using mould (Isaria fumosorosea) bacterial strain of rose dark brown rod spore as outer group, build its phylogenetic evolution tree；As a result
As shown in figure 5, the bacterial strain that the present invention is separated to is gathered in same branch with all beauveria bassianas, its affiliation is height
Spend homologous.
'beta '-tubulin gene order is compared in ncbi database, comparison result shows, the strain sequence and multiple balls
Beauveria bassiana (Beauveria bassiana) bacterial strain and one plant of muscardine (Beauveria brongniartii) bacterial strain
Sequence is highly similar.It is mould by the bacterial strain and beauveria bassiana, muscardine, some approximate bacterial strains, and with rose dark brown rod spore
(I.fumosorosea) it is outer group, builds its phylogenetic evolution tree；As a result as shown in fig. 6, the bacterial strain that is separated to of the present invention with
In same branch, its affiliation is very high homology for beauveria bassiana, one plant of muscardine.
Therefore, comprehensive ITS1-5.8S-ITS4rDNA sequences branch development tree and the development of 'beta '-tubulin gene order branch
Tree identifies that the bacterial strain is beauveria bassiana (Beauveria bassiana), and its classification position belongs to Deuteromycotina
(Deuteromycotina), Hyphomycetes (Hyphomycetes), Moniliales (Moniliales), Moniliaceae
(Moniliaceae), Beauveria (Beauveria).
By the bacterial strain and beauveria bassiana (Beauveria bassiana) BbL25 bacterial strains are named as, and in 2 months 2017
China typical culture collection center (CCTCC) is preserved within 16th, culture presevation numbering is CCTCC NO：M2017045, classification
Name number is Beauveria bassiana；Preservation address is Wuhan, China Wuhan University.
Toxicity test of the beauveria bassiana BbL25 bacterial strains of embodiment 2 to red fire ant
1st, prepared by spore suspension
(1) conidium produces
The slant strains for the beauveria bassiana BbL25 bacterial strains that Example 1 preserves, scrape a small amount of conidium and are seeded to
On PDA plate, culture to bacterium colony soon covers with culture dish in 25 DEG C of incubators, and scraping bacterium colony top layer conidium is standby.
(2) conidium suspension preparation
The conidium that step (1) scrapes is placed in centrifuge tube, adds 0.02% Tween-80 solution, vibration shakes up,
Spore concentration is measured with blood counting chamber, is configured to the conidial suspension of required various concentrations, it is standby.
It is formulated as follows the conidial suspension of the beauveria bassiana BbL25 bacterial strains of concentration：
(1)1×106Individual/mL；
(2)5×105Individual/mL；
(3)1×105Individual/mL；
(4)5×104Individual/mL；
Under the conditions of in laboratory, red fire ant is subjected to impregnation in above-mentioned 4 different concentration spore suspensions,
The death rate of continuous 14 days investigation red fire ants after processing, fatal rate, the lethal concentration of 50 of each concentration are calculated with SPSS software analysis
(LC50)；Calculation formula is as follows：
The death rate (%)=dead borer population/before processing borer population × 100%
Corrected mortality (%)=(the processing death rate-control death rate)/(1- compares the death rate) × 100%
2nd, result
As a result show, after impregnation 14 days, beauveria bassiana BbL25 bacterial strains conidium is to red fire ant virulence effect
For：1×106Individual/mL, 5 × 105Individual/mL, 1 × 105Individual/mL, 5 × 104Individual/concentration of mL 4 corresponds to corrected mortality respectively
64.77%th, 38.10%, 9.52%, 19.05% (as shown in table 1).With the softwares of SPSS 17.0 to conidium concentration with it is flourishing
Ant corrected mortality carries out regression analysis, the half lethal concentration (LC for going out beauveria bassiana after processing50) it is 8.15 × 105It is mitogenetic
Spore/mL (as shown in table 2), the results showed that, beauveria bassiana BbL25 bacterial strains are fine to the treatment effect of red fire ant.
Toxicity test result of the BbL25 bacterial strains spore suspension of the various concentrations of table 1 to red fire ant
| Concentration/ml-1 | 1×106 | 5×105 | 1×105 | 5×104 |
| Corrected mortality | 64.77% | 38.10% | 9.52% | 19.05% |
The BbL25 bacterial strains of table 2 are to red fire ant virulence probit models and its half lethal concentration (LC50)
| Model equation | Chi-square Test pb | LC50And 95% confidential interval (spore/mL) |
| Y=1.233X-7.286a | 0.695 | 8.15×105(3.04×105-2.31×106) |
a：Concentration X is changed using the logarithm that the truth of a matter is 10
b：In test of fitness of fot result, the p of pearson Chi-square Tests>0.05, illustrate that degree of fitting has no problem
The present embodiment simultaneously, also infects red fire ant to beauveria bassiana BbL25 bacterial strains and observed in biologicall test.See
It is respectively the 2nd~4 day after red fire ant death to examine the time, and in dissection Microscopic observation, it infects the morphological feature of red fire ant, and it is tied
Fruit is as shown in Figure 3：Beauveria bassiana conidial suspension dipping red fire ant after the 2nd~4 day, red fire ant occur it is slow in action,
Flagging phenomenon, then occur dead.Under an optical microscope, white hypha can be observed first from red fire ant corpse
Podarthrum, mouthpart, stretch out at body segment external, elapse over time, a large amount of white conidiums are produced on mycelia.
3 several entomogenous fungis of embodiment are to red fire ant Pathogenic Tests
1st, conidial method is produced according to step (1) in above-described embodiment 2, Inner Mongol different location soil will be come from
Middle code name is for MS-01, MS-02, MS-03, MS-05, MS-06, MS-08, MS-13, MS-22, L18, BbL25 bacterial strain and from wide
Java bacterial strain, the rose dark brown rod spore of In Guangzhou Area Bemisia tabaci bombys batryticatus separation of state area prodenia litura bombys batryticatus separation are mould (IfB01)
1.0 × 10 are configured to respectively7Individual spore/mL, in laboratory under the conditions of, with spore suspension impregnation red fire ant, processing
The death rate of continuous 14 days investigation red fire ants afterwards.
The death rate (%)=dead borer population/before processing borer population × 100%
Corrected mortality (%)=(the processing death rate-control death rate)/(1- compares the death rate) × 100%
2nd, different strains are as shown in table 3 to the corrected mortality of Bemisia tabaci；As a result show, beauveria bassiana BbL25 bacterial strains
To the corrected mortality highest of red fire ant, it is 87.29%, shows the pathogenicity very strong to red fire ant.
Measurement result (1 × 10 of the different entomogenous fungi bacterial strains of table 3 to red fire ant pathogenicity7mL)
Field control effectiveness test of the beauveria bassiana BbL25 bacterial strains of embodiment 4 to red fire ant
1st, conidial method is produced according to step (1) in above-described embodiment 2, beauveria bassiana conidium is added
In 0.02% Tween-80 solution, experimental group and control group are set by following concentration or method：
(1)1×106/ mL spore suspension；
(2)5×106/ mL spore suspension；
(3)1×107/ mL spore suspension；
(4) positive control：0.25% sulfluramid bait formulation (trade name：Termiticide emperor, Zhaoqing Guangdong city face Dinghu District rich biology system
Agent Co., Ltd)；
(5) blank control：Clear water.
Experiment is carried out in Agricultural University Of South China rather western teaching and scientific research base, if 3 processing (1 × 106, 5 × 106With 1 × 107
Spore/mL suspension), 4 ant nests are each handled, are repeated 3 times, if 0.25% sulfluramid bait formulation compares with clear water.It is first during dispenser
Ant nest is stirred with thin stick, lures a large amount of red fire ants to climb out of ant nest surface, sprays the beauveria bassiana BbL25 of various concentrations respectively
Bacterial strain conidial suspension is completely drenched up to ant nest, carries out mark after processing to ant nest.Before dispenser, and 7 after dispenser
My god, 14 days, use near the ant nest built in the bottle that traps of ham sausage trap red fire ant 30min, then take back countings, calculating red fire ant
Revision insect recluced rate and prevention effect.
Revision insect recluced rate (%)=(post processing of 1- medicines, which traps bottle and trapped, to be trapped bottle before red fire ant sum/medicine and traps red fire ant
Sum) × 100
Prevention effect (%)=100 × (processing decline rate-CK decline rates)/(100-CK decline rates)
2nd, result of the test shows, beauveria bassiana BbL25 bacterial strains have preferable prevention effect to red fire ant in the wild, with
Using the increase of spore suspension concentration, the prevention effect of this bacterial strain accordingly increases.When 1 × 107/ mL spores 7 days after administration
In 14 days, prevention effect respectively reaches 67.14% and 82.52%, (table 4) suitable with the effect of 0.25% sulfluramid bait formulation.
The beauveria bassiana BbL25 bacterial strains of table 4 are to red fire ant field control effect
Note：Letter after numeral represents the significant difference after duncan's new multiple range method (DMRT) analysis, and letter is different to be represented
With significant difference (p<0.05).
The present invention has carried out the experiment of field drug effect using spray-on process to beauveria bassiana, the chemical drugs with preventing and treating red fire ant
Agent is made comparisons, and the prevention effect of beauveria bassiana is of a relatively high, and continuation is longer.
SEQUENCE LISTING
<110>Agricultural University Of South China
<120>One plant of beauveria bassiana BbL25 bacterial strain for preventing and treating red fire ant and its application
<130> 2017
<160> 2
<170> PatentIn version 3.3
<210> 1
<211> 540
<212> DNA
<213> Beauveria bassiana BbL25
<400> 1
gtggcatcga ctcactccct acccttctgt gacctaccta tcgttgcttc ggcggactcg 60
ccccagcccg gacgcggact ggaccagcgg cccgccgggg acctcaaact cttgtattcc 120
agcatcttct gaatacgccg caaggcaaaa caaatgaatc aaaactttca acaacggatc 180
tcttggctct ggcatcgatg aagaacgcag cgaaacgcga taagtaatgt gaattgcaga 240
atccagtgaa tcatcgaatc tttgaacgca cattgcgccc gccagcattc tggcgggcat 300
gcctgttcga gcgtcatttc aaccctcgac ctccccttgg gggggtcggc gttggggacc 360
ggcagcacac cgccggccct gaaatggagt ggcggcccgt ccgcggcgac ctctgcgcag 420
taatacagct cgcaccggaa ccccgacgcg gccacgccgt aaaacaccca acttctgaac 480
gttgacctcg aatcaggtag gactacccgc tgaacttaag catatcaaaa ggcggaggaa 540
<210> 2
<211> 506
<212> DNA
<213> Beauveria bassiana BbL25
<400> 2
gggctcctgc tcctgacgag cgtggtgccc actccttccg cgccgtctct gttcccgagc 60
tcactcagca gatgttcgac cctaagaaca tgatggctgc ttctgacttc cgcaacggtc 120
gctacctgac ctgctctgcc attttgtaag ttgattcaaa catgtactga ttgacgcatc 180
aactaacaac cctttagccg tggtaaggtt gccatgaagg aggttgagga ccagatgcgt 240
aatgtgcaga ccaagaactc cagctacttc gtcgagtgga tccccaacaa catccagaac 300
gctctctgcg ccgttccccc ccgcggactt aagatgtcgt ctaccttcat tggtaactcg 360
acctctatcc aggatctctt caagcgtgtc ggtgagcagt tctccgccat gttccgtcgc 420
aaggctttcc ttcattggta cactggtgaa ggtatggacg agatggagtt cactgaggct 480
gagttcaaca tgaagaatct cgtcaa 506
Claims (9)
1. the beauveria bassiana BbL25 bacterial strains of one plant of preventing and treating red fire ant, it is characterised in that the bacterial strain was on 2 16th, 2017
China typical culture collection center is preserved in, culture presevation number is CCTCC NO：M2017045.
2. beauveria bassiana BbL25 bacterial strains according to claim 1, it is characterised in that the beauveria bassiana BbL25 bacterium
The ITS1-5.8S-ITS4 rDNA sequences such as SEQ ID NO of strain：Shown in 1.
3. beauveria bassiana BbL25 bacterial strains according to claim 1, it is characterised in that the beauveria bassiana BbL25 bacterium
The 'beta '-tubulin gene order such as SEQ ID NO of strain：Shown in 2.
4. application of the beauveria bassiana BbL25 bacterial strains in red fire ant is prevented and treated described in claim 1.
5. application according to claim 4, it is characterised in that the method for the application is：Harvest beauveria bassiana BbL25
The conidium of bacterial strain, concentration is configured to as 5 × 10 with 0.01~0.03% Tween 80 solution6~1 × 107/ ml spore suspension
Liquid, spraying suspension is carried out by Solenopsis invicta with red fire ant using spray-on process.
6. the beauveria bassiana BbL25 bacterial strains described in claim 1 are preparing the application in preventing and treating red fire ant medicament.
7. a kind of biological agent for preventing and treating red fire ant, it is characterised in that include the beauveria bassiana described in claim 1
BbL25 bacterial strains.
8. biological agent according to claim 5, it is characterised in that include 5 × 10 in the biological agent6~1 ×
107Spore/ml beauveria bassiana BbL25 bacterial strain spore suspensions.
9. application of the biological agent in red fire ant is prevented and treated described in claim 7 or 8.
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| CN113684136A (en) * | 2021-07-21 | 2021-11-23 | 华南农业大学 | Broad-spectrum insecticidal beauveria bassiana strain and application thereof |
| CN114480138A (en) * | 2022-01-11 | 2022-05-13 | 长江师范学院 | Beauveria bassiana LZ2-1a and application thereof |
| CN115927006A (en) * | 2022-10-24 | 2023-04-07 | 华南农业大学 | Beauveria bassiana and application thereof in preventing and controlling red imported fire ants |
| CN117384768A (en) * | 2023-11-30 | 2024-01-12 | 云南农业大学 | Application of sorangium elegans, conidium and metabolite thereof in prevention and treatment of solenopsis invicta |
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| CN110129209A (en) * | 2019-05-14 | 2019-08-16 | 西南林业大学 | The mould category fungal bacterial strain of the pale purple purple of one kind and its application |
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| CN113150999A (en) * | 2020-12-17 | 2021-07-23 | 华南农业大学 | Entomogenous fungus strain with high pathogenicity to prodenia litura and application thereof |
| CN113684136A (en) * | 2021-07-21 | 2021-11-23 | 华南农业大学 | Broad-spectrum insecticidal beauveria bassiana strain and application thereof |
| CN113684136B (en) * | 2021-07-21 | 2023-08-22 | 华南农业大学 | Broad-spectrum insecticidal beauveria bassiana strain and application thereof |
| CN114480138A (en) * | 2022-01-11 | 2022-05-13 | 长江师范学院 | Beauveria bassiana LZ2-1a and application thereof |
| CN114480138B (en) * | 2022-01-11 | 2023-08-18 | 长江师范学院 | Beauveria bassiana LZ2-1a and application thereof |
| CN115927006A (en) * | 2022-10-24 | 2023-04-07 | 华南农业大学 | Beauveria bassiana and application thereof in preventing and controlling red imported fire ants |
| CN117384768A (en) * | 2023-11-30 | 2024-01-12 | 云南农业大学 | Application of sorangium elegans, conidium and metabolite thereof in prevention and treatment of solenopsis invicta |
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