CN112760237B - Cladosporium cladosporioides strain with strong pathogenicity to diaphorina citri and application thereof - Google Patents
Cladosporium cladosporioides strain with strong pathogenicity to diaphorina citri and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of biological control, and particularly relates to a Cladosporium cladosporioides strain with strong pathogenicity to diaphorina citri and application thereof, wherein the Clad strain is obtained by separating abnormal dead diaphorina citri bodies of citrus plants, is identified as Cladosporium cladosporioides (Cladosporium cladosporioides), is named Cladosporium cladosporioides CLAD, is preserved in Guangdong province microorganism strain preservation center in 11-17 th 2020, has a preservation number of GDMCC No:61248, has large spore production amount, has strong pathogenicity to diaphorina citri and imagoes at normal temperature and humidity, is expected to be developed into biocontrol bacteria of diaphorina citri, and provides more choices for green biological control of diaphorina citri.
Description
Technical Field
The invention belongs to the technical field of biological control, and particularly relates to a cladosporium cladosporioides strain with strong pathogenicity to diaphorina citri and application thereof.
Background
Citrus Wood FENG (Diaphorinacitri Kuwayama) belongs to the Homoptera (Homoptera) and family Wood FENG (Chermidae), is a main pest in the young shoot stage of Rutaceae plants such as oranges, lemons, wampee, Murraya koenigii, medlar and is also the only natural insect vector for spreading citrus yellow shoot. The citrus yellow shoot is the primary problem of citrus planting industry, causes serious economic loss to fruit growers, and greatly hinders the development of citrus industry in China and even the world.
At present, chemical control measures are mostly adopted for controlling the diaphorina citri, although the purpose of killing pests is achieved by using a large amount of chemical pesticides, the frequent use of the chemical pesticides causes various problems of pesticide residue, environmental pollution, biodiversity damage, pesticide resistance of the diaphorina citri and the like. Therefore, at present, the country advocates the 'two-reduction' measure of farmland weight-reducing and drug-reducing, so that the industry is seeking a safer and pollution-free method for controlling the diaphorina citri.
The biological control method is considered as an ideal alternative scheme for future chemical control by the characteristics of high efficiency, no toxicity, no residue, no pollution, difficult generation of drug resistance, easy obtainment of raw materials and the like. Therefore, people are gradually paying attention to the biological method for controlling the diaphorina citri. The prevention and control of the diaphorina citri by the insect pathogenic bacteria can not only avoid the problem that chemical agents are easy to generate drug resistance, but also effectively solve the environmental problem caused by using a large amount of chemical agents, and is one of the biological prevention and control means with the most application prospect. At present, a wide variety of insect pathogens, mainly pathogenic fungi, bacteria and viruses, have been found. Among them, Beauveria bassiana and nucleopolyhedrosis virus are used in pest control, and the production technology is mature. However, the number of strains having insecticidal activity against diaphorina citri is relatively small, and the insecticidal activity is also relatively low, and reported main strains include paecilomyces lilacinus, paecilomyces variotii, corynebacterium fumosoroseum, lecanicillium, verticillium lecanii, and the like. But the effective utilization in the current production is still less, so that the development of more citrus psyllid biocontrol bacteria resources is particularly important for effectively preventing and controlling the citrus psyllids and the citrus greening diseases.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides the cladosporium cladosporioides strain, the cladosporium cladosporioides strain has stronger pathogenicity to the diaphorina citri under normal temperature and normal humidity, has the possibility of being developed as a biocontrol bacterium, and provides more choices for green biological control of the diaphorina citri.
In order to achieve the purpose, the invention adopts the technical scheme that:
the invention provides a Cladosporium cladosporioides CLAD which has strong pathogenicity to diaphorina citri, is preserved in Guangdong province microorganism strain preservation center at 11-17.2020, and has a preservation number of GDMCC No. 61248, wherein the rDNA-ITS2 of the CLAD strain has a nucleotide sequence shown in SEQ ID NO: 1.
The strain is obtained by separating abnormal dead diaphorina citri from citrus plants planted in a fruit tree research institute test base of Guangdong academy of agricultural sciences in Tianheu, Guangzhou, Guangdong province, and is identified as Cladostorium (Cladosporium) and Cladosporium cladosporioides (Cladosporium cladosporioides).
The invention also provides application of the cladosporium cladosporioides strain in controlling diaphorina citri.
The invention also provides application of the cladosporium cladosporioides strain in preparing biological pesticides for controlling diaphorina citri.
The invention also provides application of the cladosporium cladosporioides strain in preventing and treating plant diseases caused by diaphorina citri.
The invention also provides application of the cladosporium cladosporioides strain in preparing a biological pesticide for preventing and treating plant diseases caused by diaphorina citri.
Tests show that the Clad strain of Cladosporium cladosporium produces a large amount of spores, spore liquid has strong pathogenicity to both diaphorina citri and adults at normal temperature and humidity, and has a killing effect on diaphorina citri, and the corrected death rates of the diaphorina citri in all ages on day 7 are respectively as follows: 100% of 1 year old; 100% at 2 instars; 100% at 3 instars; 60% for 4 th instar and 30% for 5 th instar; on day 10, the corrected mortality rate for adult diaphorina citri was 83.3%. Therefore, the strain is expected to be developed into biocontrol bacteria of diaphorina citri, and more choices are provided for green biocontrol of diaphorina citri.
Preferably, the plant includes, but is not limited to, murraya jasminorage. Also includes common diaphorina citri plants, such as citrus, lemon, wampee, murraya paniculata and other rutaceae plants.
Preferably, the disease is citrus yellow dragon disease.
The invention also provides a medicament for controlling diaphorina citri, which is characterized by comprising the cladosporium cladosporioides strain.
The invention also provides a medicament for preventing and treating citrus greening disease, which is characterized by comprising the cladosporium cladosporioides strain.
Preferably, the above-mentioned pharmaceutical agent is prepared by a method comprising: inoculating the Cladosporium cladosporioides on a PDA (personal digital assistant) plate for culturing for at least 10 days, collecting hyphae and spores to prepare spore suspension, wherein the concentration of the spore suspension is (1.0 × 10)5-1.0×109) spores/mL.
Compared with the prior art, the invention has the beneficial effects that:
the CLAD strain is obtained by separating abnormal dead diaphorina citri from an orange plant, is identified as Cladosporium cladosporioides (Cladosporium cladosporioides), and is named as Cladosporium cladosporioides CLAD, the strain has high spore yield, spore liquid has strong pathogenicity to both diaphorina citri nymphs and imagoes at normal temperature and normal humidity, and the strain is expected to be developed into biocontrol bacteria of diaphorina citri and provides more choices for green biological control of the diaphorina citri.
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FIG. 1 is a graph of the growth of Clad strains of Cladosporium cladium on PDA medium;
FIG. 2 is an electron microscope image of hypha, conidiophores and conidia of ClaD strain of Clad cladosporium;
FIG. 3 is an electron microscope image of conidia of Clad strain of cladosporium cladosporioides;
FIG. 4 is an adult diaphorina citri resulting from the death of a ClaD strain of Cladosporium cladosporioides.
Detailed Description
The following further describes the embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
The experimental procedures in the following examples were carried out by conventional methods unless otherwise specified, and the test materials used in the following examples were commercially available by conventional methods unless otherwise specified.
EXAMPLE 1 isolation and purification of Strain CLAD
Collecting abnormal dead diaphorina citri kummer bodies from citrus plants planted in a fruit tree research institute test base of agricultural academy of sciences of Guangdong province, Guangzhou city, Guangdong province. The collected diaphorina citri cadavers are subjected to surface disinfection by using 75% alcohol solution, disinfected samples are washed in sterilized water for three times and are placed into a PDA (personal digital Assistant) plate, the PDA plate is placed in a thermostat at 25 ℃ upside down for culture, after bacterial colonies are formed, single bacterial colonies are selected for separation and purification, a pure bacterial strain is obtained after multiple transfer culture and is named as bacterial strain CLAD, and the pure bacterial strain is transferred into a refrigerator at 4 ℃ for storage.
Example 2 identification and preservation of Strain CLAD
Extracting total DNA of the strain CLAD by an Ezup kit, performing PCR amplification by using a fungus universal primer, and sequencing. Alignment analysis using NCBI Blast revealed that the sequence similarity between the sequences tested (ITS1-5.8S rDNA-ITS2 sequence) and multiple strains of Cladosporium cladosporioides (DCF1, daef25, GX36, FJAT-30992, etc.) in GenBank was as high as 99% to 100%. The strain CLAD was confirmed to belong to Cladosporium (Cladosporium), Cladosporium cladosporioides (Cladosporium cladosporioides), and was named as Cladosporium cladosporioides (Cladosporium cladosporioides) CLAD. Wherein the size of the rDNA-ITS2 sequence of the Clad strain of cladosporium cladosporioides is 525bp, and the nucleotide sequence is shown as SEQ ID NO: 1 is shown.
Subsequently, ClaD strain of Cladosporium cladium was taken to the Guangdong province culture Collection for registration and preservation at 17.11/2020, with the following information:
preservation time: year 2020, 11 months and 17 days;
the name of the depository: guangdong province culture Collection of microorganisms (GDMCC);
the preservation number is: GDMCC No. 61248;
the address of the depository: building No. 59, building No. 5 of the first-furious Zhonglu 100 yard in Guangzhou city;
and (3) classification and naming: cladosporium cladosporioides.
Example 3 morphological characteristics of Cladosporium cladosporioides CLAD Strain
Clad strain of Cladosporium cladosporioides grows well on potato glucose medium (PDA), as shown in figure 1, the diameter of colony can reach 6.5cm when cultured for 10 days at 27 ℃, the colony is circular, and the colony is dark green and velvet. The front surface of the colony cultured for 10 days is dark green flocculent, the middle part is light yellow and is raised, and the back surface of the colony is dark green. The hypha branch has transverse septa and branches shown in figure 2. The conidia are oval and have a size of 14.7-20.0 μm × 23.3-63.3 μm, as shown in FIG. 3.
Example 4 lethal concentration and time of lethal neutralization of Cladosporium cladonioides CLAD strains on Trialeurodes citrifolia
Inoculating Clad strain of Cladosporium cladosporioides on PDA plate, culturing for 10d, scraping hypha and spore of fungus with inoculating needle, washing with 0.03% Tween-80 sterile water, and collecting conidia. Pouring the bacterial liquid into a beaker, stirring with a magnetic stirrer, completely scattering spores, filtering with gauze to obtain relatively pure spore suspension, counting the spore concentration of the mother liquid with a blood counting chamber under a 40-fold objective lens, and respectively diluting to 1.0 × 109，1×108，1×107，1×106，1×105，1×104spore/mL for use.
Selecting healthy Murraya koenigii plants and checking to ensure that there are no diaphorina citri (including nymphs and adults), and then placing the Murraya koenigii plants in a 60cm by 60cm rearing cage. Randomly grabbing 30 citrus psylla adults to be fedFreezing in refrigerator at-20 deg.C for 30 s, soaking in the spore suspension with each concentration for 10 s, and placing the adult insects with recovered standing state in the breeding cage containing folium Et cacumen Murrayae plant. The number of deaths of the diaphorina citri adults was recorded by daily observation for 10 consecutive days. Performing regression analysis on the data obtained by the experiment through DPS software to obtain a regression equation and a correlation coefficient r, and calculating the lethal middle concentration (LD)50) And median time to death (LT)50)。
The experimental results show that the spore suspension has higher lethality to the diaphorina citri adults along with the increase of the concentration of the spore suspension. LD of Clad on Carniphylla 3 days after inoculation50Is 4.6X 108spore/mL, day 10 LD50Is 3.2X 104spores/mL. When the concentration of spore suspension is 1.0X 105LT of spores/mL against diaphorina citri508.32 days, and when the concentration is 1.0X 109In time of LT thereof50Only 2.43 days.
Example 5 pathogenicity of Clad Strain of Cladosporium cladosporioides for the nymphs of diaphorina citri at different ages
(1) The diaphorina citri used in the experiment is from the indoor net of murraya jasminorage planted by the research institute of fruit trees of agricultural academy of agriculture in Guangdong province, Tianhe area of Guangzhou city, Guangdong province.
(2) Selecting strong murraya paniculata plants, pruning the tops of the strong murraya paniculata plants, respectively putting the strong murraya paniculata plants into insect cages of 60cm multiplied by 60cm, putting 20 pairs of adult diaphorina citri when the murraya paniculata shoots to 3cm, transferring all the adults after the adults lay eggs for 24h, and carrying out tests when the nymphs of the diaphorina citri develop to different ages. Washing with 0.03% Tween-80 sterile water, collecting conidia of the strain, scattering, filtering, and diluting to 1 × 107spore/mL for use. Selecting and marking murraya jasminorage tender shoots with low-age nymphs of diaphorina citri, counting the number of the diaphorina citri nymphs at each age on each tender shoot, uniformly spraying a strain spore suspension prepared by 0.03 percent of tween-80 on the marked murraya jasminorage tender shoots, replacing a contrast with 0.03 percent of tween-80 sterile aqueous solution, and setting 3 times of repetition for each treatment. Observing and recording the death number of diaphorina citri nymphs at each age every day,the observation was continued for 7 days.
The experimental results show that the corrected mortality rates of diaphorina citri nymphs at all ages on day 3 are respectively as follows: 100% of 1 year old; 100% at 2 instars; 100% at 3 instars; 50% for 4 th instar and 20% for 5 th instar; the corrected mortality rates for diaphorina citri nymphs at each age on day 7 were: 100% of 1 year old; 100% at 2 instars; 100% at 3 instars; age 4, 60%, age 5 30%.
Example 6 pathogenicity of Cladosporium cladosporium Clad strains on adult diaphorina citri
Selecting healthy Murraya koenigii plants and checking to ensure that there are no diaphorina citri (including nymphs and adults), and then placing the Murraya koenigii plants in a 60cm by 60cm rearing cage. Randomly picking 30 adult diaphorina citri, freezing at-20 deg.C for 30 s, freezing to obtain powder, and adding into 1 × 108spores/mL of the strain were infiltrated for 10 seconds in a spore suspension, and the adults recovered from standing were then placed in the cages containing Murraya koenigii plants as described above. The number of deaths of the diaphorina citri adults was recorded by daily observation for 10 consecutive days.
The experimental results showed that the corrected mortality rate for the diaphorina citri adults was 33.3% at day 3, 70% at day 7 and 83.3% at day 10. FIG. 4 shows the adult diaphorina citri caused by the infection of Clad strain of Cladosporium cladosporioides.
The embodiments of the present invention have been described in detail, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.
Sequence listing
<110> research institute of fruit trees of Guangdong province academy of agricultural sciences
<120> cladosporium cladosporioides strain with strong pathogenicity on diaphorina citri and application thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 525
<212> DNA
<213> rDNA-ITS2 sequence (Cladosporium cladosporioides)
<400> 1
tgcggaggga tcattacaag tgaccccggt ctaaccaccg ggatgttcat aaccctttgt 60
tgtccgactc tgttgcctcc ggggcgaccc tgccttcggg cgggggctcc gggtggacac 120
ttcaaactct tgcgtaactt tgcagtctga gtaaatttaa ttaataaatt aaaactttta 180
acaacggatc tcttggttct ggcatcgatg aagaacgcag cgaaatgcga taagtaatgt 240
gaattgcaga attcagtgaa tcatcgaatc tttgaacgca cattgcgccc cctggtattc 300
cggggggcat gcctgttcga gcgtcatttc accactcaag cctcgcttgg tattgggcaa 360
cgcggtccgc cgcgtgcctc aaatcgaccg gctgggtctt ctgtccccta agcgttgtgg 420
aaactattcg ctaaagggtg ctcgggaggc tacgccgtaa aacaaaccca tttctaaggt 480
tgacctcgga tcaggtaggg atacccgctg aacttaagca tatca 525
Claims (10)
1. A Cladosporium cladosporioides strain is characterized in that the strain is Cladosporium cladosporioides (C.) (Cladosporium cladosporioides) CLAD has strong pathogenicity to diaphorina citri, is preserved in Guangdong province microorganism culture collection center in 11 months and 17 days in 2020, and has a preservation number of GDMCC No. 61248, and rDNA-ITS2 of the CLAD strain has a nucleotide sequence shown in SEQ ID NO: 1.
2. Use of a cladosporium cladosporioides strain according to claim 1 for controlling diaphorina citri.
3. Use of a cladosporium cladosporioides strain according to claim 1 for the preparation of a biopesticide for the control of diaphorina citri.
4. Use of a cladosporium cladosporioides strain according to claim 1 for controlling plant diseases caused by diaphorina citri.
5. Use of a cladosporium cladosporioides strain according to claim 1 for the preparation of a biopesticide for controlling plant diseases caused by diaphorina citri.
6. Use according to claim 4 or 5, wherein the plant is murraya paniculata.
7. Use according to claim 4 or 5, wherein the disease is citrus yellow dragon.
8. An agent for controlling diaphorina citri comprising the cladosporium cladosporioides strain of claim 1.
9. An agent for controlling citrus greening disease, comprising the cladosporium cladosporioides strain of claim 1.
10. The medicament according to claim 8 or 9, wherein the preparation method comprises: inoculating Cladosporium cladosporioides of claim 1 on PDA plate, culturing for no less than 10 days, collecting hypha and spore to obtain spore suspension with concentration of 1.0 × 105-1.0×109spores/mL.
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| CN106065392A (en) * | 2016-03-14 | 2016-11-02 | 华南农业大学 | A kind of diaphorina citri highly pathogenicity fumosorosea bacterial strain and application thereof |
| CN107667735A (en) * | 2017-09-07 | 2018-02-09 | 广东省农业科学院果树研究所 | A kind of method of effectively preventing and treating diaphorina citri |
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| CN106065392A (en) * | 2016-03-14 | 2016-11-02 | 华南农业大学 | A kind of diaphorina citri highly pathogenicity fumosorosea bacterial strain and application thereof |
| CN107667735A (en) * | 2017-09-07 | 2018-02-09 | 广东省农业科学院果树研究所 | A kind of method of effectively preventing and treating diaphorina citri |
Non-Patent Citations (2)
| Title |
|---|
| 柑橘木虱的发生及防治;廖勇军;《农村经济与科技》;20200930;第31卷(第18期);第40-41页 * |
| 柑橘黄龙病菌对柑橘木虱及其天敌的影响机制;邱海燕等;《中国热带农业科学院环境与植物保护研究所》;20180808;第1-2页 * |
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