CN107410389A - A kind of biological pesticide of effectively preventing and treating oil tea charcoal myiasis - Google Patents
A kind of biological pesticide of effectively preventing and treating oil tea charcoal myiasis Download PDFInfo
- Publication number
- CN107410389A CN107410389A CN201710471448.5A CN201710471448A CN107410389A CN 107410389 A CN107410389 A CN 107410389A CN 201710471448 A CN201710471448 A CN 201710471448A CN 107410389 A CN107410389 A CN 107410389A
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- China
- Prior art keywords
- oil tea
- seed meal
- camellia seed
- biological pesticide
- effectively preventing
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G2013/004—Liquid mulch
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
A kind of biological pesticide of effectively preventing and treating oil tea charcoal myiasis disclosed by the invention, it is related to biopharmaceutical technology, by camellia seed meal, Chinese anise, psoralea corylifolia, giant knotweed, reed, arbor-vitae is through everfermentation, extraction, mixing, allotment forms, Tea Saponin in prepared biological pesticide, flavone compound, organic acid, the antibacterial substance rich content such as alkaloid, it is active high, fungistatic effect is excellent, to oil tea leaf blight, Oil Tea Anthracnose, oil tea root rot, a variety of oil tea diseases such as oil tea soft rot have good antagonistic effect, antimicrobial spectrum is relatively broad, cost accounting can effectively be reduced.
Description
Technical field
The present invention relates to biomedicine technical field, particularly a kind of biological pesticide of effectively preventing and treating oil tea charcoal myiasis.
Background technology
Oil tea has good application and development prospect as fully natural green health food, but because China is to oil tea disease
Harmful research is started late, therefore oil tea disease turns into the bottleneck for restricting camellia oleiferaindustryization development at present.Oil tea leaf blight is oil
One of tea Major Diseases, in each main oil tea producing region in the whole nation, there is different degrees of generation.The disease can cause oil tea largely to be fallen leaves
Shedding, extreme influence is caused to the yield and quality of oil tea.The disease main cause harm Camellia Leaves, bud and fruit, once morbidity, disease
Strain rate can reach 15%-25%, and serious is even up to more than 90%；The incidence of disease of fruit also can reach 25%, and serious can
Reach more than 40%.Oil tea leaf blight is more to fall ill since blade tip or leaf margin, and sick leaf will come off after catching an illness 2-3 days, and fruit or
Leaf bud, which is caught an illness, occurs withered and yellow or dehiscent fruit, shedding phenomenon or so the latter moon, until corruption softening.The country is directed to oil tea charcoal at present
The preventing and treating of myiasis is substantially using chemical pesticide, in the case where chemical pesticide drawback increasingly highlights, some microbial source agricultures
Medicine gradually receives the highest attention of domestic and foreign scholars.The exploitation and application of microbial pesticide have been increasingly becoming pesticide industry
New trend, its main advantage are：1) species is various, and the choice of research and development is big；2) selectivity is strong, and non-target organism is pacified
Entirely, it is not likely to produce the resistance to the action of a drug；3) activity is high, and production cost is low, and zymotechnique is simple；4) it is environmentally safe, it is nuisanceless, without residual
Stay.
From the perspective of biological control, biological source agriculture safely, effectively, less toxic is researched and developed from plant extracts
Medicine, to promoting oil tea non-environmental pollution control that there is certain realistic meaning.
The content of the invention
The purpose of the present invention be for the oil tea charcoal myiasis of prior art prevention effect it is general, chemical residual is high etc. asks
A kind of topic, there is provided biological pesticide of effectively preventing and treating oil tea charcoal myiasis.It is a further object of the invention to provide the biological pesticide
Preparation method.
The biological pesticide of effectively preventing and treating oil tea charcoal myiasis of the invention, is prepared by the raw material of following mass ratio：
Camellia seed meal 30-50, Chinese anise 10-15, psoralea corylifolia 5-8, giant knotweed 3-6, reed 4-9, arbor-vitae 3-5.
The preparation method of the biological pesticide of described effective preventing and treating oil tea charcoal myiasis, comprises the following steps：
（1）The preparation of camellia seed meal extract：
A, potassium phosphate, urea are added in camellia seed meal, regulation water content is 55%-60%, and inoculated aspergillus niger, trichoderma, mixing is
After even, more than 30 DEG C of keeping temperature, it is sealed by fermentation 15-20 days, obtains fermentate；
Wherein, the addition of the potassium phosphate is the 1%-3% of camellia seed meal quality；The addition of urea is camellia seed meal quality
2%-4%；The inoculum concentration of the aspergillus niger is the 0.4%-0.8% of camellia seed meal quality；The inoculum concentration of the trichoderma is camellia seed meal
The 0.4%-0.8% of quality；
B, in fermentate, the water of 8-10 times of camellia seed meal quality is added, 20-30min is soaked, boils 10-20min, filter
To camellia seed meal leaching liquor；
（2）The preparation of Chinese medical extract：
Chinese anise, psoralea corylifolia, giant knotweed, reed, arbor-vitae are dried under reduced pressure at a temperature of 10-15 DEG C, make its moisture for 8% with
Under, crush as granularity 2mm powder, then supercritical extract is carried out, 50-60 DEG C of extraction temperature, extracting pressure 20-30MPa, extraction
Time 2-4h is taken, isolates Chinese medical extract；
（3）By step（1）Obtained camellia seed meal leaching liquor and step（2）Gained Chinese medical extract is mixed into solution A, by solution
A and water in mass ratio 1:（200-300）Mix and produce the biological pesticide.
Beneficial effects of the present invention：
Biological pesticide of the present invention is prepared simply, and rich in a variety of effective components and microbial activity composition, its fungistatic effect is good, right
The prevention effect of oil tea charcoal myiasis protrudes, and cost is cheap, and environmental pollution is small, has nuisanceless and the characteristics of without residual hazard, effectively keeps away
Exempt from the harm of chemical pesticide, and had good facilitation in the growth for oil tea, improve the yield of oil tea.
Embodiment
With reference to specific embodiment, the present invention is described in further detail.
Embodiment 1
A kind of biological pesticide of effectively preventing and treating oil tea charcoal myiasis, is prepared from the following materials：
Camellia seed meal 50kg, Chinese anise 15kg, psoralea corylifolia 8kg, giant knotweed 6kg, reed 9kg, arbor-vitae kg.
Its preparation method, comprise the following steps：
（1）The preparation of camellia seed meal extract：
A, 1.5kg potassium phosphates, 2kg urea are added in camellia seed meal, regulation water content is 60%, inoculation 0.4kg aspergillus nigers,
0.4kg trichodermas, after being well mixed, more than 30 DEG C of keeping temperature, it is sealed by fermentation 20 days, obtains fermentate；
B, in fermentate, camellia seed meal 500kg water is added, 30min is soaked, boils 20min, is filtrated to get camellia seed meal extraction
Liquid；
（2）The preparation of Chinese medical extract：
Chinese anise, psoralea corylifolia, giant knotweed, reed, arbor-vitae are dried under reduced pressure at a temperature of 15 DEG C, it is less than 8% to make its moisture,
Crush as granularity 2mm powder, then carry out supercritical extract, 60 DEG C, extracting pressure 30MPa, extraction time 4h of extraction temperature,
Isolate Chinese medical extract；
（3）By step（1）Obtained camellia seed meal leaching liquor and step（2）Gained Chinese medical extract is mixed into solution A, by solution
A and water in mass ratio 1:（200-300）Mix and produce the biological pesticide.
The controlling experiment of the present invention of test example 2
In Tongren oil tea planting base, the serious plantation of oil tea charcoal myiasis, and landform, the oil tea standing forest height of tree, hat width are selected
Camellia oleifera lam etc. condition no significant difference is tested.Camellia oleifera lam no good kind is tested, susceptible oil tea charcoal myiasis, is adopted after afforestation
With normal Tending measures.Experiment uses RANDOMIZED BLOCK DESIGN, sets 2 district's groups, and control group uses commercially available 50% wettable
500-800 times of liquid of thiophanate；Test group is sprayed using biological pesticide of the present invention.18 oil tea seedling of each fertilizer treatment.In April
The last ten-days period are sprayed, and are then sprayed every two months once, and other management are carried out in the usual way.(the April 15 in 2012 before spraying
Day) and trees when stopping growing (on October 25th, 2012), situation, calculating preventive effect are checked in each test process cell, and survey
Fresh pod yield.
Result of the test
| Test group | Control group | |
| Prevention effect | 96.58% | 87.32% |
| Fresh pod yield（kg/hm2） | 17945.6 | 14308.3 |
As can be seen that compared to the control group using commercially available agricultural chemicals, the preventing and treating of oil tea charcoal myiasis after biological pesticide of the present invention is used
Effect has had more 9.26 percentage points, and fresh pod yield improves 25.42%, its significant effect.
Claims (2)
1. a kind of biological pesticide of effectively preventing and treating oil tea charcoal myiasis, it is characterised in that be prepared by the raw material of following mass ratio：
Camellia seed meal 30-50, Chinese anise 10-15, psoralea corylifolia 5-8, giant knotweed 3-6, reed 4-9, arbor-vitae 3-5.
2. the preparation method of the biological pesticide of effectively preventing and treating oil tea charcoal myiasis as claimed in claim 1, it is characterised in that including
Following steps：
（1）The preparation of camellia seed meal extract：
A, potassium phosphate, urea are added in camellia seed meal, regulation water content is 55%-60%, and inoculated aspergillus niger, trichoderma, mixing is
After even, more than 30 DEG C of keeping temperature, it is sealed by fermentation 15-20 days, obtains fermentate；
Wherein, the addition of the potassium phosphate is the 1%-3% of camellia seed meal quality；The addition of urea is camellia seed meal quality
2%-4%；The inoculum concentration of the aspergillus niger is the 0.4%-0.8% of camellia seed meal quality；The inoculum concentration of the trichoderma is camellia seed meal
The 0.4%-0.8% of quality；
B, in fermentate, the water of 8-10 times of camellia seed meal quality is added, 20-30min is soaked, boils 10-20min, filter
To camellia seed meal leaching liquor；
（2）The preparation of Chinese medical extract：
Chinese anise, psoralea corylifolia, giant knotweed, reed, arbor-vitae are dried under reduced pressure at a temperature of 10-15 DEG C, make its moisture for 8% with
Under, crush as granularity 2mm powder, then supercritical extract is carried out, 50-60 DEG C of extraction temperature, extracting pressure 20-30MPa, extraction
Time 2-4h is taken, isolates Chinese medical extract；
（3）By step（1）Obtained camellia seed meal leaching liquor and step（2）Gained Chinese medical extract is mixed into solution A, by solution
A and water in mass ratio 1:（200-300）Mix and produce the biological pesticide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710471448.5A CN107410389A (en) | 2017-06-20 | 2017-06-20 | A kind of biological pesticide of effectively preventing and treating oil tea charcoal myiasis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710471448.5A CN107410389A (en) | 2017-06-20 | 2017-06-20 | A kind of biological pesticide of effectively preventing and treating oil tea charcoal myiasis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107410389A true CN107410389A (en) | 2017-12-01 |
Family
ID=60427332
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710471448.5A Pending CN107410389A (en) | 2017-06-20 | 2017-06-20 | A kind of biological pesticide of effectively preventing and treating oil tea charcoal myiasis |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108077330A (en) * | 2017-12-20 | 2018-05-29 | 罗山县灵山茶业有限责任公司 | Prevent biological pesticide of oil tea pest and disease damage and preparation method thereof |
Citations (5)
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|---|---|---|---|---|
| CN1618293A (en) * | 2003-11-20 | 2005-05-25 | 郭伟光 | Non-pollution agricultural chemicals, and prepn. method therefor |
| CN101485338A (en) * | 2008-01-18 | 2009-07-22 | 邹如 | Green food pesticide composition and uses thereof |
| CN103168803A (en) * | 2013-03-28 | 2013-06-26 | 陈士进 | Plant source bacteriostasis and detoxification deodorant and preparation method and application of plant source bacteriostasis and detoxification deodorant |
| CN105053082A (en) * | 2015-07-15 | 2015-11-18 | 刘晓 | Drug for preventing and treating michelia figo leaf blight |
| CN106490028A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of agricultural chemicals of preventing and treating elimination of underground destructive pests and preparation method thereof |
-
2017
- 2017-06-20 CN CN201710471448.5A patent/CN107410389A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1618293A (en) * | 2003-11-20 | 2005-05-25 | 郭伟光 | Non-pollution agricultural chemicals, and prepn. method therefor |
| CN101485338A (en) * | 2008-01-18 | 2009-07-22 | 邹如 | Green food pesticide composition and uses thereof |
| CN103168803A (en) * | 2013-03-28 | 2013-06-26 | 陈士进 | Plant source bacteriostasis and detoxification deodorant and preparation method and application of plant source bacteriostasis and detoxification deodorant |
| CN105053082A (en) * | 2015-07-15 | 2015-11-18 | 刘晓 | Drug for preventing and treating michelia figo leaf blight |
| CN106490028A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of agricultural chemicals of preventing and treating elimination of underground destructive pests and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108077330A (en) * | 2017-12-20 | 2018-05-29 | 罗山县灵山茶业有限责任公司 | Prevent biological pesticide of oil tea pest and disease damage and preparation method thereof |
| CN108077330B (en) * | 2017-12-20 | 2020-06-19 | 罗山县灵山茶业有限责任公司 | Biological pesticide for preventing and treating diseases and insect pests of camellia oleifera and preparation method thereof |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171201 |
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| WD01 | Invention patent application deemed withdrawn after publication |