CN107409528A - A kind of germination accelerating method of shepherd's purse seed - Google Patents
A kind of germination accelerating method of shepherd's purse seed Download PDFInfo
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- CN107409528A CN107409528A CN201710305280.0A CN201710305280A CN107409528A CN 107409528 A CN107409528 A CN 107409528A CN 201710305280 A CN201710305280 A CN 201710305280A CN 107409528 A CN107409528 A CN 107409528A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention mainly relates to planting technology field, discloses a kind of germination accelerating method of shepherd's purse seed, including：Seed immersion, seed fermentation, seed enzymolysis, presprouting of seeds, seed sowing；Method is simple, easy to operation, the germination time of shepherd's purse seed is shorten to 35 ~ 40 hours, the time for germination of being after planting unearthed is 3 ~ 4 days, and germination percentage reaches 92.4%, hence it is evident that improves plantation efficiency, saves planting cost；Shepherd's purse seed is placed in progress low temperature immersion in edible alkali solution, improves seed pH, weakens dormancy species activity, gradual breaking dormancy, improves the germinating capacity for displaying seed, accelerate germination；White granulated sugar is added into immersion seed, access lactic acid bacteria, first high temperature promotes growing microorganism, fermented when low temperature is long again, promote kind of soft and soggyization, neutralize the dietary alkali remained in seed, increase the nutritional ingredient of small molecule, caused nisin can suppress varied bacteria growing, avoid the mold of seed from rotting, and improve plantation efficiency.
Description
Technical field
The invention mainly relates to planting technology field, more particularly to a kind of germination accelerating method of shepherd's purse seed.
Background technology
Shepherd's purse, also known as probationer nurse grass, dish, rice dish, water caltrop dish etc., nutritive value is very high, rich in protein, vitamin,
Mineral element and Flavonoid substances, it can build up resistance, hemostasis and pain-relieving, protection cardiovascular and cerebrovascular, promotion gastrointestinal function, toxin expelling are supported
Face, anti-aging.
With favor more and more higher of the people to shepherd's purse, the bitter cress in field and roadside can not meet the need of people
Ask, it is necessary to carry out large-scale planting to shepherd's purse, shepherd's purse seed needs to carry out vernalization before planting, and to break seed dormancy, but passes
The shepherd's purse seed of system needs the just meeting rudiment of 8 ~ 9 days seeds through dirt bed area method or in 2~7 DEG C of low temperature refrigerator during vernalization, broadcast
It can be emerged within 4 ~ 5 days after kind, and germination percentage can only achieve 80% or so, hence it is evident that reduce plantation efficiency, therefore, it is necessary to a kind of add
The germination accelerating method of fast shepherd's purse germination.
The content of the invention
The defects of in order to make up prior art, it is an object of the invention to provide a kind of germination accelerating method of shepherd's purse seed.
A kind of germination accelerating method of shepherd's purse seed, comprises the following steps：
（1）Seed soaks：Ripe, full shepherd's purse seed is selected, is placed in the edible alkali solution that mass concentration is 1.6 ~ 1.8%,
5 ~ 6 hours are stood in 1 ~ 4 DEG C, improves seed pH, weakens dormancy species activity, gradual breaking dormancy, accelerates germination, mistake
Filter, clean, obtain immersion seed；
（2）Seed fermentation：The white granulated sugar of immersion seed weight 20 ~ 25% is added into immersion seed, adds biogas water, stirring
Dissolving, the mass concentration for making white granulated sugar are 7 ~ 9%, access lactic acid bacteria, are well mixed, fermented 4 ~ 5 hours in 42 ~ 44 DEG C, promote bacterium
Body is bred, then cools the temperature to 26 ~ 28 DEG C, ferment at constant temperature 15 ~ 17 hours, promotes kind of soft and soggyization, neutralizes the food remained in seed
With alkali, increase the nutritional ingredient of small molecule, be easy to neutron absorption, accelerate germination, nisin caused by lactobacillus-fermented
Rhzomorph can suppress varied bacteria growing, avoid the mold of seed from rotting, and improve percentage of seedgermination, and quickening is after planting unearthed, and improves plantation
Efficiency, obtain fermentation seed；
（3）Seed digests：Fermentation seed is gradually heating to 38 ~ 40 DEG C, adds complex enzyme, is well mixed, insulation enzymolysis 40 ~ 50
Minute, promote kind of a skin further to soften, enter beneficial to nutrition and moisture, and high temperature, short time processing is carried out after low temperature, accelerate to stop
Dormancy material inactivates, and breaks seed dormancy, accelerates vernalization, filters, and cleans, obtains enzymolysis seed；
（4）Presprouting of seeds：Enzymolysis seed is placed in germinating box, 1 ~ 8 hour, temperature was 5 ~ 7 DEG C, ambient humidity is 81 ~ 83%, light
According to for 60 ~ 80lx, 9 ~ 21 hours, temperature was 16 ~ 18 DEG C, ambient humidity is that 76 ~ 78%, illumination is 110 ~ 130lx, 22 hours and
After, temperature is 19 ~ 20 DEG C, ambient humidity is that 73 ~ 75%, illumination is 160 ~ 180lx, first low temperature, high humidity, dim light, promotes seed
Fully water suction, break seed dormancy, accelerate germination, then temperature and illumination are improved, improve seed resistance, stimulate intraseminal
Plumule activity, accelerate germination, then temperature and illumination are improved, strengthen the photosynthesis of seed, promote plumule growth, shorten
Germination time, obtain vernalization seed；
（5）Seed is sowed：Soil deep tillage rake is thin, base manure is used sufficient, shepherd's purse seed bar is sowed in soil, obtains sowing seed.
The step（2）Biogas water, be by former biogas water release after, filter cleaner, add former methane-generating pit water body
Product 1/4 ~ 1/3, the acetum that volume fraction is 1.4 ~ 1.6%, are heated to 60 ~ 70 DEG C, are incubated 10 ~ 15 minutes, are cooled to room
Temperature, obtain biogas water.
The step（2）Lactic acid bacteria, be made up of the raw material of following parts by weight：Lactobacillus acidophilus 16 ~ 18, Lactobacillus delbrueckii
12 ~ 14, streptococcus thermophilus 12 ~ 14, lactobacillus bulgaricus 5 ~ 7.
The step（3）Complex enzyme, after addition mass concentration be 0.04 ~ 0.06%, by the raw material group of following parts by weight
Into：Cellulase 17 ~ 19, pectase 14 ~ 16, hemicellulase 11 ~ 13, lignoenzyme 7 ~ 9, lipase 4 ~ 6.
It is an advantage of the invention that：A kind of germination accelerating method of shepherd's purse seed provided by the invention, method is simple, easy to operation,
The germination time of shepherd's purse seed is set to shorten to 35 ~ 40 hours, the time for germination of being after planting unearthed is 3 ~ 4 days, and germination percentage reaches
92.4%, hence it is evident that improve plantation efficiency, save planting cost；Shepherd's purse seed is placed in progress low temperature immersion in edible alkali solution, carried
High seed pH, weaken dormancy species activity, gradual breaking dormancy, improve the germinating capacity for displaying seed, accelerate germination；To
White granulated sugar is added in immersion seed, accesses lactic acid bacteria, and first high temperature promotes growing microorganism, then fermentation when low temperature carries out long, promotes kind
Soft and soggyization, the dietary alkali remained in seed is neutralized, increases the nutritional ingredient of small molecule, be easy to neutron absorption, accelerate germination,
Nisin caused by lactobacillus-fermented can suppress varied bacteria growing, avoid the mold of seed from rotting, and improve percentage of seedgermination,
Quickening is after planting unearthed, and improves plantation efficiency；Seed is heated and digested after fermentation, promotes kind of a skin further to soften, profit
Enter in nutrition and moisture, and high temperature, short time processing is carried out after low temperature, accelerate dormancy material inactivation, break seed dormancy, add
Fast vernalization；Constantly regulate vernalization condition during vernalization, first low temperature, high humidity, dim light, promote seed fully to absorb water, break seed dormancy,
Accelerate germination, then temperature and illumination improved, improve seed resistance, stimulate intraseminal plumule activity, accelerate germination,
Temperature and illumination are improved again, strengthen the photosynthesis of seed, promotes plumule growth, shortens germination time.
Embodiment
Illustrate the present invention with specific embodiment below.
Embodiment 1
A kind of germination accelerating method of shepherd's purse seed, comprises the following steps：
（1）Seed soaks：Ripe, full shepherd's purse seed is selected, is placed in the edible alkali solution that mass concentration is 1.6%, in 1
DEG C stand 5 hours, improve seed pH, weaken dormancy species activity, gradual breaking dormancy, accelerate germination, filter, clean,
Seed must be soaked；
（2）Seed fermentation：The white granulated sugar of immersion seed weight 20% is added into immersion seed, adds biogas water, stirring is molten
Solution, the mass concentration for making white granulated sugar are 7%, access lactic acid bacteria, are well mixed, are fermented 4 hours in 42 DEG C, promote growing microorganism, then
26 DEG C are cooled the temperature to, ferment at constant temperature 15 hours, promotes kind of soft and soggyization, neutralizes the dietary alkali remained in seed, increases small molecule
Nutritional ingredient, be easy to neutron absorption, accelerate germination, nisin can suppress miscellaneous bacteria caused by lactobacillus-fermented
Growth, avoids the mold of seed from rotting, and improves percentage of seedgermination, and quickening is after planting unearthed, and is improved plantation efficiency, is obtained fermentation seed；
（3）Seed digests：Fermentation seed is gradually heating to 38 DEG C, adds complex enzyme, is well mixed, insulation enzymolysis 40 minutes,
Promote kind of a skin further to soften, enter beneficial to nutrition and moisture, and high temperature, short time processing is carried out after low temperature, accelerate dormancy material
Inactivation, break seed dormancy, accelerate vernalization, filter, clean, obtain enzymolysis seed；
（4）Presprouting of seeds：Enzymolysis seed is placed in germinating box, 1 ~ 8 hour, temperature was 5 DEG C, ambient humidity 81%, illumination are
60lx, 9 ~ 21 hours, temperature was 16 DEG C, ambient humidity 76%, illumination 110lx, 22 hours and after, temperature is 19 DEG C, ring
Border humidity is 73%, illumination 160lx, first low temperature, high humidity, dim light, promotes seed fully to absorb water, and breaks seed dormancy, accelerates hair
Bud, then temperature and illumination are improved, improves seed resistance, stimulates intraseminal plumule activity, accelerates germination, then by temperature
Degree and illumination improve, and strengthen the photosynthesis of seed, promote plumule growth, shorten germination time, obtain vernalization seed；
（5）Seed is sowed：Soil deep tillage rake is thin, base manure is used sufficient, shepherd's purse seed bar is sowed in soil, obtains sowing seed.
The step（2）Biogas water, be by former biogas water release after, filter cleaner, add former methane-generating pit water body
Product 1/4 ~ 1/3, the acetum that volume fraction is 1.4%, are heated to 60 DEG C, are incubated 10 minutes, are cooled to room temperature, obtain methane-generating pit
Water.
The step（2）Lactic acid bacteria, be made up of the raw material of following parts by weight：Lactobacillus acidophilus 16, Lactobacillus delbrueckii 12,
Streptococcus thermophilus 12, lactobacillus bulgaricus 5.
The step（3）Complex enzyme, after addition mass concentration be 0.04%, be made up of the raw material of following parts by weight：Fiber
Plain enzyme 17, pectase 14, hemicellulase 11, lignoenzyme 7, lipase 4.
Embodiment 2
A kind of germination accelerating method of shepherd's purse seed, comprises the following steps：
（1）Seed soaks：Ripe, full shepherd's purse seed is selected, is placed in the edible alkali solution that mass concentration is 1.7%, in 2
DEG C stand 5 hours, improve seed pH, weaken dormancy species activity, gradual breaking dormancy, accelerate germination, filter, clean,
Seed must be soaked；
（2）Seed fermentation：The white granulated sugar of immersion seed weight 22% is added into immersion seed, adds biogas water, stirring is molten
Solution, the mass concentration for making white granulated sugar are 8%, access lactic acid bacteria, are well mixed, are fermented 4 hours in 43 DEG C, promote growing microorganism, then
27 DEG C are cooled the temperature to, ferment at constant temperature 16 hours, promotes kind of soft and soggyization, neutralizes the dietary alkali remained in seed, increases small molecule
Nutritional ingredient, be easy to neutron absorption, accelerate germination, nisin can suppress miscellaneous bacteria caused by lactobacillus-fermented
Growth, avoids the mold of seed from rotting, and improves percentage of seedgermination, and quickening is after planting unearthed, and is improved plantation efficiency, is obtained fermentation seed；
（3）Seed digests：Fermentation seed is gradually heating to 39 DEG C, adds complex enzyme, is well mixed, insulation enzymolysis 45 minutes,
Promote kind of a skin further to soften, enter beneficial to nutrition and moisture, and high temperature, short time processing is carried out after low temperature, accelerate dormancy material
Inactivation, break seed dormancy, accelerate vernalization, filter, clean, obtain enzymolysis seed；
（4）Presprouting of seeds：Enzymolysis seed is placed in germinating box, 1 ~ 8 hour, temperature was 6 DEG C, ambient humidity 82%, illumination are
70lx, 9 ~ 21 hours, temperature was 17 DEG C, ambient humidity 77%, illumination 120lx, 22 hours and after, temperature is 19.5 DEG C,
Ambient humidity is 74%, illumination 170lx, first low temperature, high humidity, dim light, promotes seed fully to absorb water, breaks seed dormancy, is accelerated
Germination, then temperature and illumination are improved, seed resistance is improved, stimulates intraseminal plumule activity, accelerates germination, then will
Temperature and illumination improve, and strengthen the photosynthesis of seed, promote plumule growth, shorten germination time, obtain vernalization seed；
（5）Seed is sowed：Soil deep tillage rake is thin, base manure is used sufficient, shepherd's purse seed bar is sowed in soil, obtains sowing seed.
The step（2）Biogas water, be by former biogas water release after, filter cleaner, add former methane-generating pit water body
Product 1/4 ~ 1/3, the acetum that volume fraction is 1.5%, are heated to 65 DEG C, are incubated 13 minutes, are cooled to room temperature, obtain methane-generating pit
Water.
The step（2）Lactic acid bacteria, be made up of the raw material of following parts by weight：Lactobacillus acidophilus 17, Lactobacillus delbrueckii 13,
Streptococcus thermophilus 13, lactobacillus bulgaricus 6.
The step（3）Complex enzyme, after addition mass concentration be 0.05%, be made up of the raw material of following parts by weight：Fiber
Plain enzyme 18, pectase 15, hemicellulase 12, lignoenzyme 8, lipase 5.
Embodiment 3
A kind of germination accelerating method of shepherd's purse seed, comprises the following steps：
（1）Seed soaks：Ripe, full shepherd's purse seed is selected, is placed in the edible alkali solution that mass concentration is 1.8%, in 4
DEG C stand 6 hours, improve seed pH, weaken dormancy species activity, gradual breaking dormancy, accelerate germination, filter, clean,
Seed must be soaked；
（2）Seed fermentation：The white granulated sugar of immersion seed weight 25% is added into immersion seed, adds biogas water, stirring is molten
Solution, the mass concentration for making white granulated sugar are 9%, access lactic acid bacteria, are well mixed, are fermented 5 hours in 44 DEG C, promote growing microorganism, then
28 DEG C are cooled the temperature to, ferment at constant temperature 17 hours, promotes kind of soft and soggyization, neutralizes the dietary alkali remained in seed, increases small molecule
Nutritional ingredient, be easy to neutron absorption, accelerate germination, nisin can suppress miscellaneous bacteria caused by lactobacillus-fermented
Growth, avoids the mold of seed from rotting, and improves percentage of seedgermination, and quickening is after planting unearthed, and is improved plantation efficiency, is obtained fermentation seed；
（3）Seed digests：Fermentation seed is gradually heating to 40 DEG C, adds complex enzyme, is well mixed, insulation enzymolysis 50 minutes,
Promote kind of a skin further to soften, enter beneficial to nutrition and moisture, and high temperature, short time processing is carried out after low temperature, accelerate dormancy material
Inactivation, break seed dormancy, accelerate vernalization, filter, clean, obtain enzymolysis seed；
（4）Presprouting of seeds：Enzymolysis seed is placed in germinating box, 1 ~ 8 hour, temperature was 7 DEG C, ambient humidity 83%, illumination are
80lx, 9 ~ 21 hours, temperature was 18 DEG C, ambient humidity 78%, illumination 130lx, 22 hours and after, temperature is 20 DEG C, ring
Border humidity is 75%, illumination 180lx, first low temperature, high humidity, dim light, promotes seed fully to absorb water, and breaks seed dormancy, accelerates hair
Bud, then temperature and illumination are improved, improves seed resistance, stimulates intraseminal plumule activity, accelerates germination, then by temperature
Degree and illumination improve, and strengthen the photosynthesis of seed, promote plumule growth, shorten germination time, obtain vernalization seed；
（5）Seed is sowed：Soil deep tillage rake is thin, base manure is used sufficient, shepherd's purse seed bar is sowed in soil, obtains sowing seed.
The step（2）Biogas water, be by former biogas water release after, filter cleaner, add former methane-generating pit water body
Product 1/4 ~ 1/3, the acetum that volume fraction is 1.6%, are heated to 70 DEG C, are incubated 15 minutes, are cooled to room temperature, obtain methane-generating pit
Water.
The step（2）Lactic acid bacteria, be made up of the raw material of following parts by weight：Lactobacillus acidophilus 18, Lactobacillus delbrueckii 14,
Streptococcus thermophilus 14, lactobacillus bulgaricus 7.
The step（3）Complex enzyme, after addition mass concentration be 0.06%, be made up of the raw material of following parts by weight：Fiber
Plain enzyme 19, pectase 16, hemicellulase 13, lignoenzyme 9, lipase 6.
Comparative example 1
Removal step（1）, remaining method, with embodiment 1.
Comparative example 2
Removal step（2）, remaining method, with embodiment 1.
Comparative example 3
Removal step（3）, remaining method, with embodiment 1.
Comparative example 4
Step（4）In vernalization condition be always maintained at temperature be 5 DEG C, ambient humidity 81%, illumination 60lx, remaining method, together
Embodiment 1.
Comparative example 5
The low-temperature germination method of existing traditional shepherd's purse seed.
The Germination effect of embodiment and comparative example shepherd's purse seed accelerating germination method：
In Anhui Province Bengbu Region, 8000, the shepherd's purse seed for displaying 2 years of mature and plump is selected, is randomly divided into 8 groups, every group
1000, respectively embodiment group and comparative example group, each group carry out shepherd's purse presprouting of seeds, vernalization 36 according to the method for the group respectively
The seed that shows money or valuables one carries unintentionally is counted during hour, vernalization rate is calculated, is planted after vernalization, other management method all sames, is calculated within after planting 4 days
Be unearthed germination percentage, and the Germination effect of embodiment and comparative example shepherd's purse seed accelerating germination method is shown in Table 1.
Table 1：The Germination effect of embodiment and comparative example shepherd's purse seed accelerating germination method
| Project | Seed number（） | Vernalization number（） | Germinative number（Strain） | Germination percentage（%） |
| Embodiment 1 | 1000 | 546 | 913 | 91.3 |
| Embodiment 2 | 1000 | 524 | 924 | 92.4 |
| Embodiment 3 | 1000 | 538 | 918 | 91.8 |
| Comparative example 1 | 1000 | 451 | 736 | 73.6 |
| Comparative example 2 | 1000 | 463 | 753 | 75.3 |
| Comparative example 3 | 1000 | 425 | 795 | 79.5 |
| Comparative example 4 | 1000 | 368 | 757 | 75.7 |
| Comparative example 5 | 1000 | 249 | 513 | 51.3 |
Show from the result of table 1, the germination accelerating method of the shepherd's purse seed of embodiment, germination time is substantially short compared with comparative example, vernalization rate
Height, unearthed germination percentage after planting is substantially compared with comparative example height, it is possible to increase displays the germination percentage of shepherd's purse seed, saves plantation money
Source, illustrating the germination accelerating method of shepherd's purse seed provided by the invention has good Germination effect.
Claims (4)
1. a kind of germination accelerating method of shepherd's purse seed, it is characterised in that comprise the following steps：
（1）Seed soaks：Ripe, full shepherd's purse seed is selected, is placed in the edible alkali solution that mass concentration is 1.6 ~ 1.8%,
5 ~ 6 hours are stood in 1 ~ 4 DEG C, is filtered, cleans, obtains immersion seed；
（2）Seed fermentation：The white granulated sugar of immersion seed weight 20 ~ 25% is added into immersion seed, adds biogas water, stirring
Dissolving, the mass concentration for making white granulated sugar are 7 ~ 9%, access lactic acid bacteria, are well mixed, are fermented 4 ~ 5 hours in 42 ~ 44 DEG C, then by temperature
Degree is down to 26 ~ 28 DEG C, ferment at constant temperature 15 ~ 17 hours, obtains fermentation seed；
（3）Seed digests：Fermentation seed is gradually heating to 38 ~ 40 DEG C, adds complex enzyme, is well mixed, insulation enzymolysis 40 ~ 50
Minute, filter, clean, obtain enzymolysis seed；
（4）Presprouting of seeds：Enzymolysis seed is placed in germinating box, 1 ~ 8 hour, temperature was 5 ~ 7 DEG C, ambient humidity is 81 ~ 83%, light
According to for 60 ~ 80lx, 9 ~ 21 hours, temperature was 16 ~ 18 DEG C, ambient humidity is that 76 ~ 78%, illumination is 110 ~ 130lx, 22 hours and
After, temperature is 19 ~ 20 DEG C, ambient humidity is that 73 ~ 75%, illumination is 160 ~ 180lx, obtains vernalization seed；
（5）Seed is sowed：Soil deep tillage rake is thin, base manure is used sufficient, shepherd's purse seed bar is sowed in soil, obtains sowing seed.
2. the germination accelerating method of shepherd's purse seed according to claim 1, it is characterised in that the step（2）Biogas water, be
After former biogas water is released, filter cleaner, former methane-generating pit water volume 1/4 ~ 1/3 is added, the vinegar that volume fraction is 1.4 ~ 1.6%
Acid solution, 60 ~ 70 DEG C are heated to, are incubated 10 ~ 15 minutes, are cooled to room temperature, obtain biogas water.
3. the germination accelerating method of shepherd's purse seed according to claim 1, it is characterised in that the step（2）Lactic acid bacteria, by with
The raw material composition of lower parts by weight：Lactobacillus acidophilus 16 ~ 18, Lactobacillus delbrueckii 12 ~ 14, streptococcus thermophilus 12 ~ 14, bulgarian milk
Bacillus 5 ~ 7.
4. the germination accelerating method of shepherd's purse seed according to claim 1, it is characterised in that the step（3）Complex enzyme, addition
Mass concentration is 0.04 ~ 0.06% afterwards, is made up of the raw material of following parts by weight：Cellulase 17 ~ 19, pectase 14 ~ 16, half fibre
Tie up plain enzyme 11 ~ 13, lignoenzyme 7 ~ 9, lipase 4 ~ 6.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101898915A (en) * | 2009-05-26 | 2010-12-01 | 北京嘉博文生物科技有限公司 | Microbial agent suitable for crops in seedling stage and preparation method thereof |
-
2017
- 2017-05-03 CN CN201710305280.0A patent/CN107409528A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101898915A (en) * | 2009-05-26 | 2010-12-01 | 北京嘉博文生物科技有限公司 | Microbial agent suitable for crops in seedling stage and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 孙洪波，张海清，赵梁军: "《园林植物繁殖原理与技术》", 30 June 2009, 中国农业科学技术出版社 * |
| 张春庆，王建华: "《种子检验学》", 31 January 2006, 高等教育出版社 * |
| 梁称福: "《4种野菜种子的发芽特性及无菌播种培养研究》", 《广东农业科学》 * |
| 蔡冬元: "《苗木生产技术》", 31 August 2012, 机械工业出版社 * |
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Application publication date: 20171201 |