CN107397146A - A kind of preparation method of egg yolk polypeptide product - Google Patents
A kind of preparation method of egg yolk polypeptide product Download PDFInfo
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- CN107397146A CN107397146A CN201710477780.2A CN201710477780A CN107397146A CN 107397146 A CN107397146 A CN 107397146A CN 201710477780 A CN201710477780 A CN 201710477780A CN 107397146 A CN107397146 A CN 107397146A
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- disperse materials
- polypeptide product
- egg yolk
- yolk
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- 210000002969 egg yolk Anatomy 0.000 title claims abstract description 54
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 49
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 49
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 47
- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 32
- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 32
- 235000013345 egg yolk Nutrition 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 62
- 239000000047 product Substances 0.000 claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 241000235395 Mucor Species 0.000 claims abstract description 15
- 235000013601 eggs Nutrition 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 13
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000012141 concentrate Substances 0.000 claims abstract description 10
- 238000001035 drying Methods 0.000 claims abstract description 10
- 238000000703 high-speed centrifugation Methods 0.000 claims abstract description 10
- 239000006228 supernatant Substances 0.000 claims abstract description 10
- 238000000605 extraction Methods 0.000 claims description 41
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 22
- 239000012530 fluid Substances 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 11
- 238000005119 centrifugation Methods 0.000 claims description 9
- 238000011081 inoculation Methods 0.000 claims description 9
- 239000004744 fabric Substances 0.000 claims description 8
- 239000004753 textile Substances 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 3
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000008468 bone growth Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000037180 bone health Effects 0.000 abstract description 2
- 238000000855 fermentation Methods 0.000 abstract description 2
- 230000004151 fermentation Effects 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 238000005194 fractionation Methods 0.000 abstract 1
- 239000004519 grease Substances 0.000 abstract 1
- 238000000194 supercritical-fluid extraction Methods 0.000 abstract 1
- 239000000843 powder Substances 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 3
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 235000010445 lecithin Nutrition 0.000 description 2
- 229940067606 lecithin Drugs 0.000 description 2
- 239000000787 lecithin Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 1
- 101000693916 Gallus gallus Albumin Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 229960004373 acetylcholine Drugs 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000004641 brain development Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000005961 cardioprotection Effects 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L15/00—Egg products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to polypeptide product to obtain field, more particularly to a kind of preparation method of egg yolk polypeptide product, egg is cooked, obtain yolk, crush supercritical fluid extraction and fractionation grease, after residue dealcoholysis, low temperature drying disperses, 600 700 times of liquid of glutamine are uniformly sprayed on to disperse materials, it is inoculated with mucor fermentation, until disperse materials surface grows one layer of fine and closely woven fine hair, disperse materials and water are mixed, high speed centrifugation processing, remove residue, concentrated supernatant, concentrate is spray-dried, obtain egg yolk polypeptide product, the present invention, which makes egg yolk polypeptide product, has manufacture craft simple, equipment requirement degree is not high, cost is low, polypeptide yield is high, the advantages of purity is high, the polypeptide product obtained is promoting bone growth, effect is notable in terms of keeping the function of bone health.
Description
Technical field
The present invention relates to polypeptide product to obtain field, and in particular to a kind of preparation method of egg yolk polypeptide product.
Background technology
Egg is considered as tonic by the common people of state, and often edible egg can strengthen memory, can also cardioprotection and artery
Blood vessel, pre- anti-cancer, anti-aging.Chicken Albumin and yolk are each advantageous, there is substantial amounts of phosphorus, also many iron in yolk.
Meanwhile all lecithin is all from yolk in egg, and lecithin can provide choline, and help synthesizes a kind of important nerve
Mediator --- acetylcholine.So the first diatery supplement of baby, is exactly often egg yolk.Yolk is beneficial to child's benefit iron, to child
The brain development of son is also beneficial.
A kind of extract of the egg yolk polypeptide as yolk, have been shown to have and promote bone growth, keep bone health
Function, and be widely used in dairy products, beverage, functional food and health food, long-term egg yolk polypeptide of taking in can promote
Enter the growth of bone.
Egg yolk polypeptide acquisition process is primarily present cost of manufacture height at present, and polypeptide acquisition rate is low, it is low to obtain Purity
The problem of.
The content of the invention
It is an object of the invention to provide a kind of preparation method of egg yolk polypeptide product.
The technical solution adopted by the present invention is a kind of preparation method of egg yolk polypeptide product, and step is as follows：
（1）Egg is cooked, yolk is obtained, is crushed to 60-65 mesh, 1 is pressed with 80% ethanol:4-5 volume ratio mixing, loads super
Critical CO2 fluid extraction systems are extracted, and extraction temperature is 40-42 DEG C, extracting pressure 30-35MPa, extraction time
For 40-45 minutes；
（2）Extraction residue is reclaimed, after dealcoholysis, low temperature drying to water content decentralized processing in 10-12%, feeding high speed dispersor
28-30 minutes, obtain disperse materials；
（3）600-700 times of liquid of glutamine is uniformly sprayed on to disperse materials, regulation disperse materials humidity is in 90-92%, inoculation Mucor
Bacterium, set environment temperature are 16-19 DEG C, and one layer of ventilative textile cloth is covered on disperse materials upper strata, until disperse materials surface grows one
The fine and closely woven fine hair of layer；
（4）Disperse materials and water are pressed 1:6-7 volume ratio mixes, and is handled with 5000-6000r/min centrifugation rate high speed centrifugation
8-10 minutes, residue is removed, the 1/6-1/5 of concentrated supernatant to original volume, concentrate is spray-dried, it is more to obtain yolk
Peptides products.
Preferably, egg is cooked, obtains yolk, be crushed to 65 mesh, 1 is pressed with 80% ethanol:5 volume ratio mixing, loads
Supercritical CO 2 fluid extraction system is extracted, and extraction temperature is 40 DEG C, extracting pressure 35MPa, extraction time 42
Minute.
Preferably, to uniform sprinkling 620 times of liquid of glutamine on disperse materials.
Preferably, mucor inoculative proportion is 3-4 ‰.
Beneficial effect of the present invention is, the present invention, which makes egg yolk polypeptide product, has that manufacture craft is simple, equipment requirement degree
The advantages of not high, cost is low, and polypeptide yield is high, and purity is high, the polypeptide product obtained are promoting bone growth, are keeping bone to be good for
Effect is notable in terms of the function of health；
Specifically, the present invention is first through step（1）Processing, oily constituents in yolk are removed, and are obtained beneficial to subsequent handling processing
Obtain polypeptide product；
Step（1）Obtained residue is handled after high speed dispersor is scattered at a high speed, improves the dispersiveness of protein in residue, profit
When subsequent inoculations mucor is handled, effectively fully utilized by mucor, obtain polypeptide；
Disperse materials humidity is adjusted by the use of 600-700 times of liquid of glutamine as humidity regulation liquid, it is possible to increase mucor activity, promotes hair
Mould fast breeding, within a short period of time disperse materials surface can grow one layer of fine and closely woven fine hair, reduce remaining miscellaneous bacteria to disperse materials
Contaminative, improve the purity of final gained polypeptide product.
Embodiment
Embodiment 1：A kind of preparation method of egg yolk polypeptide product, specifically,
（1）Egg is cooked, yolk is obtained, is crushed to 60 mesh, 1 is pressed with 80% ethanol:4 volume ratio mixing, loads overcritical
CO2 fluid extraction systems are extracted, and extraction temperature is 40 DEG C, extracting pressure 30MPa, and extraction time is 40 minutes；
（2）Extraction residue is reclaimed, after dealcoholysis, low temperature drying to water content decentralized processing 28 in 10%, feeding high speed dispersor is divided
Clock, obtain disperse materials；
（3）Uniformly sprinkling 600 times of liquid of glutamine, regulation disperse materials humidity are inoculated with mucor, inoculation 90% on to disperse materials
Ratio is 3 ‰, and set environment temperature is 16 DEG C, and one layer of ventilative textile cloth is covered on disperse materials upper strata, until disperse materials surface is grown
Go out one layer of fine and closely woven fine hair；
（4）Disperse materials and water are pressed 1:6 volume ratio mixes, and is handled 8 minutes with 5000r/min centrifugation rate high speed centrifugation,
Removing residue, the 1/6 of concentrated supernatant to original volume, concentrate is spray-dried, obtains egg yolk polypeptide product.
Embodiment 2：A kind of preparation method of egg yolk polypeptide product, specifically,
（1）Egg is cooked, yolk is obtained, is crushed to 62 mesh, 1 is pressed with 80% ethanol:4.6 volume ratio mixing, loads overcritical
CO2 fluid extraction systems are extracted, and extraction temperature is 41 DEG C, extracting pressure 32MPa, and extraction time is 43 minutes；
（2）Extraction residue is reclaimed, after dealcoholysis, low temperature drying to water content decentralized processing 29 in 11%, feeding high speed dispersor is divided
Clock, obtain disperse materials；
（3）Uniformly sprinkling 650 times of liquid of glutamine, regulation disperse materials humidity are inoculated with mucor, inoculation 91% on to disperse materials
Ratio is 3.5 ‰, and environment temperature is 17 DEG C, and one layer of ventilative textile cloth is covered on disperse materials upper strata, until disperse materials surface is grown
One layer of fine and closely woven fine hair；
（4）Disperse materials and water are pressed 1:6.5 volume ratio is mixed, and 9 points are handled with 5200r/min centrifugation rate high speed centrifugation
Clock, removing residue, the 1/6 of concentrated supernatant to original volume, concentrate is spray-dried, obtains egg yolk polypeptide product.
Embodiment 3：A kind of preparation method of egg yolk polypeptide product, specifically,
（1）Egg is cooked, yolk is obtained, is crushed to 65 mesh, 1 is pressed with 80% ethanol:5 volume ratio mixing, loads overcritical
CO2 fluid extraction systems are extracted, and extraction temperature is 42 DEG C, extracting pressure 35MPa, and extraction time is 45 minutes；
（2）Extraction residue is reclaimed, after dealcoholysis, low temperature drying to water content decentralized processing 30 in 12%, feeding high speed dispersor is divided
Clock, obtain disperse materials；
（3）Uniformly sprinkling 700 times of liquid of glutamine, regulation disperse materials humidity are inoculated with mucor, inoculation 92% on to disperse materials
Ratio is 4 ‰, and set environment temperature is 19 DEG C, and one layer of ventilative textile cloth is covered on disperse materials upper strata, until disperse materials surface is grown
Go out one layer of fine and closely woven fine hair；
（4）Disperse materials and water are pressed 1:7 volume ratio mixes, and is handled 10 minutes with 6000r/min centrifugation rate high speed centrifugation,
Removing residue, the 1/5 of concentrated supernatant to original volume, concentrate is spray-dried, obtains egg yolk polypeptide product.
Below in conjunction with specific contrast test, the present invention is further described：
Egg is produced from a collection of same kind chicken of plant buying, after egg is cooked, yolk is taken, is crushed to 65 mesh, it is random etc.
It is divided into experimental group, control group 1, control group 2, control group 3 and amounts to 4 groups；
Wherein, experimental group obtains egg yolk polypeptide product according to following steps：
（1）Yolk powder and 80% ethanol are pressed 1:5 volume ratio mixing, loads supercritical CO 2 fluid extraction system and is extracted
Take, extraction temperature is 42 DEG C, extracting pressure 35MPa, and extraction time is 45 minutes；
（2）Extraction residue is reclaimed, after dealcoholysis, low temperature drying to water content decentralized processing 30 in 12%, feeding high speed dispersor is divided
Clock, obtain disperse materials；
（3）Uniformly sprinkling 700 times of liquid of glutamine, regulation disperse materials humidity are inoculated with mucor, inoculation 92% on to disperse materials
Ratio is 4 ‰, and set environment temperature is 19 DEG C, and one layer of ventilative textile cloth is covered on disperse materials upper strata, until disperse materials surface is grown
Go out one layer of fine and closely woven fine hair；
（4）Disperse materials and water are pressed 1:7 volume ratio mixes, and is handled 10 minutes with 6000r/min centrifugation rate high speed centrifugation,
Removing residue, the 1/5 of concentrated supernatant to original volume, concentrate is spray-dried, obtains egg yolk polypeptide product；
Wherein, control group 1 obtains egg yolk polypeptide product according to following steps：
（1）By yolk powder low temperature drying to water content 12%, decentralized processing 30 minutes in high speed dispersor are sent into, are disperseed
Material；
（2）Uniformly sprinkling 700 times of liquid of glutamine, regulation disperse materials humidity are inoculated with mucor, inoculation 92% on to disperse materials
Ratio is 4 ‰, and set environment temperature is 19 DEG C, and one layer of ventilative textile cloth is covered on disperse materials upper strata, until disperse materials surface is grown
Go out one layer of fine and closely woven fine hair；
（3）Disperse materials and water are pressed 1:7 volume ratio mixes, and is handled 10 minutes with 6000r/min centrifugation rate high speed centrifugation,
Removing residue, the 1/5 of concentrated supernatant to original volume, concentrate is spray-dried, obtains egg yolk polypeptide product；
Wherein, control group 2 obtains egg yolk polypeptide product according to following steps：
（1）Yolk powder and 80% ethanol are pressed 1:5 volume ratio mixing, loads supercritical CO 2 fluid extraction system and is extracted
Take, extraction temperature is 42 DEG C, extracting pressure 35MPa, and extraction time is 45 minutes；
（2）Extraction residue is reclaimed, after dealcoholysis, low temperature drying to water content decentralized processing 30 in 12%, feeding high speed dispersor is divided
Clock, obtain disperse materials；
（3）Uniform spray water on to disperse materials, regulation disperse materials humidity are inoculated with mucor 92%, and inoculative proportion is 4 ‰, setting
Environment temperature is 19 DEG C, and one layer of ventilative textile cloth is covered on disperse materials upper strata, until disperse materials surface grows one layer of fine and closely woven fine hair；
（4）Disperse materials and water are pressed 1:7 volume ratio mixes, and is handled 10 minutes with 6000r/min centrifugation rate high speed centrifugation,
Removing residue, the 1/5 of concentrated supernatant to original volume, concentrate is spray-dried, obtains egg yolk polypeptide product；
Wherein, control group 3 obtains egg yolk polypeptide product according to following steps：
（1）Yolk powder and 80% ethanol are pressed 1:5 volume ratio mixing, loads supercritical CO 2 fluid extraction system and is extracted
Take, extraction temperature is 42 DEG C, extracting pressure 35MPa, and extraction time is 45 minutes；
（2）Extraction residue is reclaimed, after dealcoholysis, low temperature drying to water content decentralized processing 30 in 12%, feeding high speed dispersor is divided
Clock, obtain disperse materials；
（3）Protease is added after disperse materials are dissolved in the usual way fully to digest；
（4）Zymolyte is handled 10 minutes with 6000r/min centrifugation rate high speed centrifugation, removes residue, concentrated supernatant to original
The 1/5 of volume, is spray-dried to concentrate, obtains egg yolk polypeptide product；
The final polypeptide product pick-up rate of above each group is counted, obtains the purity of polypeptide product and experimental group, control group 1, right
According to group 2 in processing procedure, the time required to disperse materials surface grows one layer of identical highly fine and closely woven fine hair, 1 as a result see the table below：
| Group | Experimental group | Control group 1 | Control group 2 | Control group 3 |
| Polypeptide product yield（mg/g） | 1.22 | 1.04 | 1.20 | 1.08 |
| Polypeptide product purity（%） | 90.5 | 75.7 | 80.3 | 83.7 |
| The time required to disperse materials surface grows one layer of identical highly fine and closely woven fine hair（h） | 18.2 | 18.5 | 55 | - |
As shown in Table 1, yolk powder and 80% ethanol are pressed 1:5 volume ratio mixing, loads supercritical CO 2 fluid extraction system
Extracted, extraction temperature be 42 DEG C, extracting pressure 35MPa, extraction time be 45 minutes to improve egg yolk polypeptide yield with
And purity has certain help, to disperse materials on uniformly sprinkling 700 times of liquid of glutamine grow one layer of phase to shortening disperse materials surfaces
There is notable help the time required to highly fine and closely woven fine hair, inoculation mucor fermentation obtains polypeptide and is significantly better than in terms of polypeptide yield
Protease hydrolyzed yield is directly inoculated with, is also no less than in terms of Purity and is directly inoculated with protease enzyme purity.
Claims (4)
1. a kind of preparation method of egg yolk polypeptide product, it is characterised in that step is as follows：
（1）Egg is cooked, yolk is obtained, is crushed to 60-65 mesh, 1 is pressed with 80% ethanol:4-5 volume ratio mixing, loads super
Critical CO2 fluid extraction systems are extracted, and extraction temperature is 40-42 DEG C, extracting pressure 30-35MPa, extraction time
For 40-45 minutes；
（2）Extraction residue is reclaimed, after dealcoholysis, low temperature drying to water content decentralized processing in 10-12%, feeding high speed dispersor
28-30 minutes, obtain disperse materials；
（3）600-700 times of liquid of glutamine is uniformly sprayed on to disperse materials, regulation disperse materials humidity is in 90-92%, inoculation Mucor
Bacterium, set environment temperature are 16-19 DEG C, and one layer of ventilative textile cloth is covered on disperse materials upper strata, until disperse materials surface grows one
The fine and closely woven fine hair of layer；
（4）Disperse materials and water are pressed 1:6-7 volume ratio mixes, and is handled with 5000-6000r/min centrifugation rate high speed centrifugation
8-10 minutes, residue is removed, the 1/6-1/5 of concentrated supernatant to original volume, concentrate is spray-dried, it is more to obtain yolk
Peptides products.
2. the preparation method of a kind of egg yolk polypeptide product according to claim 1, it is characterised in that step is as follows：
Egg is cooked, yolk is obtained, is crushed to 65 mesh, 1 is pressed with 80% ethanol:5 volume ratio mixing, loads supercritical CO 2
Fluid extraction system is extracted, and extraction temperature is 40 DEG C, extracting pressure 35MPa, and extraction time is 42 minutes.
3. the preparation method of a kind of egg yolk polypeptide product according to claim 1, it is characterised in that step is as follows：
620 times of liquid of glutamine are uniformly sprayed on to disperse materials.
4. the preparation method of a kind of egg yolk polypeptide product according to claim 1, it is characterised in that step is as follows：
Mucor inoculative proportion is 3-4 ‰.
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2017
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| JP6045678B1 (en) * | 2015-10-19 | 2016-12-14 | 大韓民国 国立水産科学院Ｒｅｐｕｂｌｉｃ Ｏｆ Ｋｏｒｅａ（Ｎａｔｉｏｎａｌ Ｆｉｓｈｅｒｉｅｓ Ｒｅｓｅａｒｃｈ Ａｎｄ Ｄｅｖｅｌｏｐｍｅｎｔ Ｉｎｓｔｉｔｕｔｅ） | Antibacterial peptides derived from abalone β-glucan binding protein, nucleic acids encoding the same, and uses thereof |
| CN106244658A (en) * | 2016-09-19 | 2016-12-21 | 陕西科技大学 | A kind of preparation method of sweet potato polypeptide |
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